Cp4.1LG12g09230.1 (mRNA) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG12g09230.1 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 981.9 bits (2537), Expect = 4.2e-285
Identity = 492/772 (63.73%), Postives = 594/772 (76.94%), Query Frame = 0

Query: 8   SSVFFFF--FLCFLPVIFSRSSDKQETFIVHVSRSEKPSLFSSHYRWHSSILDSLSPSSR 67
           SS+FF F   LCF     S SSD  E++IVHV RS KPSLFSSH  WH S+L SL  S +
Sbjct: 7   SSIFFVFPLLLCFFSP-SSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQ 66

Query: 68  PRKLLYNYERAANGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFLGLADNLG 127
           P  LLY+Y RA +GF+AR++  Q A LRR P V+SVIPD+ R++HTT TP FLG + N G
Sbjct: 67  PATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSG 126

Query: 128 LWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATTCNRKIIG 187
           LW ++NY +DVI+GVLDTGIWPE PSFSD GL P+P++WKG CE G  F A++CNRK+IG
Sbjct: 127 LWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIG 186

Query: 188 ARAFFHGYESNIR-RPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARGEARG 247
           ARAF+ GY +       H   + +S RD++GHGTHTASTAAGS V NAS +QYARG A G
Sbjct: 187 ARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATG 246

Query: 248 MASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGA 307
           MAS+ARIAAYKICW  GCYDSDILAAMDQA+ADGVH+ISLSVG+SG AP Y+ DSIAIGA
Sbjct: 247 MASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGA 306

Query: 308 FGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSL 367
           FGA +HGIVVSCSAGNSGP P TA NIAPWILTVGAST+DREF A+ I GDG+VF+G SL
Sbjct: 307 FGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSL 366

Query: 368 YSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAVKSAGG 427
           Y+G+ L DS+L LVY GDCG+R CY G L+ S V GKIV+CDRGGNARV KGSAVK AGG
Sbjct: 367 YAGESLPDSQLSLVYSGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKLAGG 426

Query: 428 LGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTVISDSP 487
            G+ILANT E+GEEL ADSHL+P TM+G  AG+++RDYI +  +P+A I F GT+I  SP
Sbjct: 427 AGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSP 486

Query: 488 PSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFNIISGT 547
           PSPRVAAFSSRGPN+ T  ILKPDVIAPGVNILAGW+G   P+ L ID RRV+FNIISGT
Sbjct: 487 PSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIISGT 546

Query: 548 SMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSNPFVHGG 607
           SMSCPHVSG+AALLRKA P W+PAAIKSAL+TT+Y ++ SG PI+DL+T   SN F+HG 
Sbjct: 547 SMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGA 606

Query: 608 GHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCE-HKLSNPGNL 667
           GH+DPNKALNPGL+YD+  ++YV+FLC++GY+   I VF++D +  + CE  KL   G+L
Sbjct: 607 GHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDL 666

Query: 668 NYPSFAVVFTSNGEEVVKYTRTVTNVGDKADAVYEVKVEAPQGIEISVLPNKLEFSAKKT 727
           NYPSF+VVF S G EVVKY R V NVG   DAVYEV V++P  +EI V P+KL FS +K+
Sbjct: 667 NYPSFSVVFASTG-EVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKS 726

Query: 728 TQSYEITFTKI-----SGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIAS 771
              YE+TF  +      G      FGSI+W+DG H+V+SP+AV +   S+ S
Sbjct: 727 VLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQWGQGSVQS 776

BLAST of Cp4.1LG12g09230.1 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 777.7 bits (2007), Expect = 1.2e-223
Identity = 415/764 (54.32%), Postives = 530/764 (69.37%), Query Frame = 0

Query: 8   SSVFFFFFLCFLPVIFSRSSDKQETFIVHVSRSEKPSLFSSHYRWHSSILDSLSPSSRPR 67
           SS  FF  LC      S SS  Q T+IVH+++S+ PS F  H  W+ S L S+S S+   
Sbjct: 7   SSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSA--- 66

Query: 68  KLLYNYERAANGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFLGLADNLG-L 127
           +LLY YE A +GF+ R+T  +A  L   PGV+SV+P+   +LHTTRTP FLGL ++   L
Sbjct: 67  ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL 126

Query: 128 WPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATTCNRKIIGA 187
           +P+     DV++GVLDTG+WPE  S+SDEG  P+P+SWKG CEAG  F+A+ CNRK+IGA
Sbjct: 127 FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGA 186

Query: 188 RAFFHGYESNIRRPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARGEARGMA 247
           R F  GYES +  P+    + +S RD DGHGTHT+STAAGS V+ AS   YA G ARGMA
Sbjct: 187 RFFARGYESTM-GPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMA 246

Query: 248 SRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFG 307
            RAR+A YK+CW  GC+ SDILAA+D+AIAD V+++S+S+G  G    YYRD +AIGAF 
Sbjct: 247 PRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLG--GGMSDYYRDGVAIGAFA 306

Query: 308 AMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYS 367
           AM+ GI+VSCSAGN+GP   +  N+APWI TVGA T+DR+F A  ILG+G+ F+GVSL+ 
Sbjct: 307 AMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFK 366

Query: 368 GDPLGDSKLPLVYGGDCGN----RYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAVKSA 427
           G+ L D  LP +Y G+  N      C +G+L P KV GKIV+CDRG NARV KG  VK+A
Sbjct: 367 GEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAA 426

Query: 428 GGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTVISD 487
           GG+G+ILANT  NGEEL+AD+HL+P T +GE AG+ +R Y+ +DPNP+A+I   GTV+  
Sbjct: 427 GGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVG- 486

Query: 488 SPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFNIIS 547
             PSP VAAFSSRGPN  T  ILKPD+IAPGVNILA W+G   P+ L+ D+RRVEFNIIS
Sbjct: 487 VKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIIS 546

Query: 548 GTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSNPFVH 607
           GTSMSCPHVSG+AALL+   P W+PAAI+SAL+TT+Y     G+P+ D++T   S PF H
Sbjct: 547 GTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDH 606

Query: 608 GGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCE-HKLSNPG 667
           G GH+ P  A NPGLIYDL  +DY+ FLC++ Y S QI   V   +Y+  C+  K  +  
Sbjct: 607 GAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIR-SVSRRNYT--CDPSKSYSVA 666

Query: 668 NLNYPSFAVVFTSNGEEVVKYTRTVTNVGDKADAVYEVKVEA-PQGIEISVLPNKLEFSA 727
           +LNYPSFAV    +G    KYTRTVT+VG      Y VKV +   G++ISV P  L F  
Sbjct: 667 DLNYPSFAV--NVDGVGAYKYTRTVTSVG--GAGTYSVKVTSETTGVKISVEPAVLNFKE 726

Query: 728 KKTTQSYEITFTKISGF-KNSASFGSIQWSDGSHIVRSPIAVSF 764
               +SY +TFT  S     S SFGSI+WSDG H+V SP+A+S+
Sbjct: 727 ANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of Cp4.1LG12g09230.1 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 743.4 bits (1918), Expect = 2.5e-213
Identity = 388/740 (52.43%), Postives = 508/740 (68.65%), Query Frame = 0

Query: 30  QETFIVHVSRSEKPSLFSSHYRWHSSILDSLSPSSRPRKLLYNYERAANGFAARITDAQA 89
           ++T+I+ V+ S+KP  F +H+ W++S L+S S       LLY Y  + +GF+A +   +A
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES------SLLYTYTTSFHGFSAYLDSTEA 86

Query: 90  -AELRRVPGVVSVIPDRMRQLHTTRTPHFLGLADNLGLWPDTNYADDVIIGVLDTGIWPE 149
            + L     ++ +  D +  LHTTRTP FLGL    G+    + ++ VIIGVLDTG+WPE
Sbjct: 87  DSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPE 146

Query: 150 RPSFSDEGLSPVPASWKGTCEAGEGFSATTCNRKIIGARAFFHGYESNIRRPLHGLSDFK 209
             SF D  +  +P+ WKG CE+G  F +  CN+K+IGAR+F  G++           +  
Sbjct: 147 SRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESV 206

Query: 210 SARDSDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRARIAAYKICWEFGCYDSDIL 269
           S RD DGHGTHT++TAAGS V+NASF  YA G ARGMA+RAR+A YK+CW  GC+ SDIL
Sbjct: 207 SPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDIL 266

Query: 270 AAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGAMQHGIVVSCSAGNSGPGPYTA 329
           AAMD+AI DGV ++SLS+G  G AP YYRD+IAIGAF AM+ G+ VSCSAGNSGP   + 
Sbjct: 267 AAMDRAILDGVDVLSLSLG-GGSAP-YYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASV 326

Query: 330 VNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSGDPLGDSKLPLVY--GGDCGNR 389
            N+APW++TVGA T+DR+F A   LG+G+  +GVSLYSG  +G   L LVY  G    + 
Sbjct: 327 ANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGNSSSSN 386

Query: 390 YCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAVKSAGGLGVILANTEENGEELLADSHLI 449
            C  GSLD S V GKIVVCDRG NARV KG+ V+ AGGLG+I+ANT  +GEEL+ADSHL+
Sbjct: 387 LCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLL 446

Query: 450 PGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTVISDSPPSPRVAAFSSRGPNYRTAEILK 509
           P   +G+  G+ LR+Y+ SD  P+A +VF+GTV+ D  PSP VAAFSSRGPN  T EILK
Sbjct: 447 PAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVL-DVKPSPVVAAFSSRGPNTVTPEILK 506

Query: 510 PDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFNIISGTSMSCPHVSGVAALLRKAFPTWT 569
           PDVI PGVNILAGWS    P+ L  D+RR +FNI+SGTSMSCPH+SG+A LL+ A P W+
Sbjct: 507 PDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWS 566

Query: 570 PAAIKSALITTSYSLDTSGRPIKDLSTSNDSNPFVHGGGHIDPNKALNPGLIYDLNPQDY 629
           P+AIKSAL+TT+Y LD +  P+ D + ++ SNP+ HG GH+DP KAL+PGL+YD++ ++Y
Sbjct: 567 PSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEY 626

Query: 630 VSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSNPGNLNYPSFAVVFTSNGEEVVKYTRTV 689
           + FLCS+ Y    I   VK  S +  C  K S+PG LNYPSF+V+F   G+ VV+YTR V
Sbjct: 627 IRFLCSLDYTVDHIVAIVKRPSVN--CSKKFSDPGQLNYPSFSVLF--GGKRVVRYTREV 686

Query: 690 TNVGDKADAVYEVKVEAPQGIEISVLPNKLEFSAKKTTQSYEITFTKISG--FKNSASFG 749
           TNVG  A +VY+V V     + ISV P+KL F +    + Y +TF    G    N A FG
Sbjct: 687 TNVG-AASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFG 746

Query: 750 SIQWSDGSHIVRSPIAVSFN 765
           SI WS+  H VRSP+A S+N
Sbjct: 747 SITWSNPQHEVRSPVAFSWN 752

BLAST of Cp4.1LG12g09230.1 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 3.8e-209
Identity = 390/773 (50.45%), Postives = 515/773 (66.62%), Query Frame = 0

Query: 11  FFFFFLCFLPVIFSRSSDKQETFIVHVSRSEKPSLFSSHYRWHSSILDSLSPSSRPRKLL 70
           +FFF L       S SS    T+IVHV    KPS+F +H+ W++S L SL  +S P  ++
Sbjct: 6   YFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASL--TSSPPSII 65

Query: 71  YNYERAANGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFLGL--ADNLGLWP 130
           + Y+   +GF+AR+T   A++L   P V+SVIP+++R LHTTR+P FLGL   D  GL  
Sbjct: 66  HTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLE 125

Query: 131 DTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATTCNRKIIGARA 190
           ++++  D++IGV+DTG+WPERPSF D GL PVP  WKG C A + F  + CNRK++GAR 
Sbjct: 126 ESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARF 185

Query: 191 FFHGYESNIRRPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARGEARGMASR 250
           F  GYE+     ++  ++F+S RDSDGHGTHTAS +AG +V  AS   YA G A GMA +
Sbjct: 186 FCGGYEAT-NGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPK 245

Query: 251 ARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGAM 310
           AR+AAYK+CW  GCYDSDILAA D A+ADGV +ISLSVG  G    YY D+IAIGAFGA+
Sbjct: 246 ARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVG--GVVVPYYLDAIAIGAFGAI 305

Query: 311 QHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSGD 370
             GI VS SAGN GPG  T  N+APW+ TVGA TIDR+F A+V LG+G++ SGVS+Y G 
Sbjct: 306 DRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGP 365

Query: 371 PLGDSKL-PLVYGGDC--GNRY----CYSGSLDPSKVAGKIVVCDRGGNARVAKGSAVKS 430
            L   ++ PLVYGG    G+ Y    C  GSLDP+ V GKIV+CDRG N+R  KG  V+ 
Sbjct: 366 GLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRK 425

Query: 431 AGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYI------HSDPNPSATIVF 490
            GGLG+I+AN   +GE L+AD H++P T +G   G+++R YI       S  +P+ATIVF
Sbjct: 426 NGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVF 485

Query: 491 RGTVISDSPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRR 550
           +GT +    P+P VA+FS+RGPN  T EILKPDVIAPG+NILA W     PS ++ D RR
Sbjct: 486 KGTRLG-IRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRR 545

Query: 551 VEFNIISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSN 610
            EFNI+SGTSM+CPHVSG+AALL+ A P W+PAAI+SALITT+Y++D SG P+ D ST N
Sbjct: 546 TEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGN 605

Query: 611 DSNPFVHGGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEH 670
            S+   +G GH+ P KA++PGL+YD+   DY++FLC+  Y    I    +  +  +    
Sbjct: 606 TSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCD-GAR 665

Query: 671 KLSNPGNLNYPSFAVVFTSNGEEVVK--YTRTVTNVGDKADAVYEVKVEAPQGIEISVLP 730
           +  + GNLNYPSF+VVF   GE  +   + RTVTNVGD +D+VYE+K+  P+G  ++V P
Sbjct: 666 RAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGD-SDSVYEIKIRPPRGTTVTVEP 725

Query: 731 NKLEFSAKKTTQSYEI----TFTKISGFKNSASFGSIQWSDGSHIVRSPIAVS 763
            KL F       S+ +    T  K+S    +   G I WSDG   V SP+ V+
Sbjct: 726 EKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770

BLAST of Cp4.1LG12g09230.1 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 703.7 bits (1815), Expect = 2.2e-201
Identity = 373/767 (48.63%), Postives = 497/767 (64.80%), Query Frame = 0

Query: 8   SSVFFFFFLCFLPVIFSRSSDKQETFIVHVSRSEKPSLFSSHYRWHSSILDSLSPSSRPR 67
           S++    FL F P I   +S   +TFI  +     PS+F +HY W+S      +  +   
Sbjct: 4   STIVLLLFLSF-PFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYS------TEFAEES 63

Query: 68  KLLYNYERAANGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFLGLADNLGLW 127
           ++++ Y    +GF+A +T  +A  LR  P V++V  DR R+LHTTR+P FLGL +  GLW
Sbjct: 64  RIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLW 123

Query: 128 PDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATTCNRKIIGAR 187
            +++Y  DVIIGV DTGIWPER SFSD  L P+P  W+G CE+G  FS   CNRKIIGAR
Sbjct: 124 SESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGAR 183

Query: 188 AFFHGYESNIRRPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARGEARGMAS 247
            F  G ++ +   ++   +F S RD+DGHGTHT+STAAG     AS   YA G A+G+A 
Sbjct: 184 FFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAP 243

Query: 248 RARIAAYKICW-EFGCYDSDILAAMDQAIADGVHIISLSV-GSSGRAPAYYRDSIAIGAF 307
           +ARIAAYK+CW + GC DSDILAA D A+ DGV +IS+S+ G  G    YY D IAIG++
Sbjct: 244 KARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSY 303

Query: 308 GAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLY 367
           GA   GI VS SAGN GP   +  N+APW+ TVGASTIDR F AD ILGDG    GVSLY
Sbjct: 304 GAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLY 363

Query: 368 SGDPLGDSKLPLVYGGDCG---NRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAVKSA 427
           +G PL     P+VY G  G      C   +LDP +V GKIV+CDRG + RVAKG  VK A
Sbjct: 364 AGVPLNGRMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKA 423

Query: 428 GGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTVISD 487
           GG+G+ILAN   NGE L+ D+HLIP   +G   G++++ Y  S PNP A+I FRGT++  
Sbjct: 424 GGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVG- 483

Query: 488 SPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFNIIS 547
             P+P +A+FS RGPN  + EILKPD+IAPGVNILA W+    P+ L  D R+ EFNI+S
Sbjct: 484 IKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILS 543

Query: 548 GTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSNPFVH 607
           GTSM+CPHVSG AALL+ A P W+PA I+SA++TT+  +D S R + D ST   + P+ +
Sbjct: 544 GTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDY 603

Query: 608 GGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSNPGN 667
           G GH++  +A+NPGL+YD+   DY++FLCSIGY  + I V  +  +       +  +PGN
Sbjct: 604 GSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITR--TPVRCPTTRKPSPGN 663

Query: 668 LNYPSFAVVFTSNGEEVVKYT--RTVTNVGDKADAVYEVKVEAPQGIEISVLPNKLEFSA 727
           LNYPS   VF +N   +V  T  RT TNVG +A+AVY  ++E+P+G+ ++V P +L F++
Sbjct: 664 LNYPSITAVFPTNRRGLVSKTVIRTATNVG-QAEAVYRARIESPRGVTVTVKPPRLVFTS 723

Query: 728 KKTTQSYEITFT----KISGFKNSASFGSIQWSD-GSHIVRSPIAVS 763
               +SY +T T     +   +  A FGS+ W D G H+VRSPI V+
Sbjct: 724 AVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVT 759

BLAST of Cp4.1LG12g09230.1 vs. NCBI nr
Match: XP_023548434.1 (subtilisin-like protease SBT1.4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1538 bits (3982), Expect = 0.0
Identity = 771/771 (100.00%), Postives = 771/771 (100.00%), Query Frame = 0

Query: 1   MANFHRCSSVFFFFFLCFLPVIFSRSSDKQETFIVHVSRSEKPSLFSSHYRWHSSILDSL 60
           MANFHRCSSVFFFFFLCFLPVIFSRSSDKQETFIVHVSRSEKPSLFSSHYRWHSSILDSL
Sbjct: 1   MANFHRCSSVFFFFFLCFLPVIFSRSSDKQETFIVHVSRSEKPSLFSSHYRWHSSILDSL 60

Query: 61  SPSSRPRKLLYNYERAANGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFLGL 120
           SPSSRPRKLLYNYERAANGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFLGL
Sbjct: 61  SPSSRPRKLLYNYERAANGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFLGL 120

Query: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATTCN 180
           ADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATTCN
Sbjct: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATTCN 180

Query: 181 RKIIGARAFFHGYESNIRRPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARG 240
           RKIIGARAFFHGYESNIRRPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARG
Sbjct: 181 RKIIGARAFFHGYESNIRRPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARG 240

Query: 241 EARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
           EARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI
Sbjct: 241 EARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300

Query: 301 AIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS 360
           AIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS
Sbjct: 301 AIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS 360

Query: 361 GVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAVK 420
           GVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAVK
Sbjct: 361 GVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAVK 420

Query: 421 SAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTVI 480
           SAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTVI
Sbjct: 421 SAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTVI 480

Query: 481 SDSPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFNI 540
           SDSPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFNI
Sbjct: 481 SDSPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSNPF 600
           ISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSNPF 600

Query: 601 VHGGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSNP 660
           VHGGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSNP
Sbjct: 601 VHGGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSNP 660

Query: 661 GNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDKADAVYEVKVEAPQGIEISVLPNKLEFSA 720
           GNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDKADAVYEVKVEAPQGIEISVLPNKLEFSA
Sbjct: 661 GNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDKADAVYEVKVEAPQGIEISVLPNKLEFSA 720

Query: 721 KKTTQSYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM 771
           KKTTQSYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM
Sbjct: 721 KKTTQSYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM 771

BLAST of Cp4.1LG12g09230.1 vs. NCBI nr
Match: XP_022953749.1 (subtilisin-like protease SBT1.4 [Cucurbita moschata])

HSP 1 Score: 1521 bits (3939), Expect = 0.0
Identity = 763/772 (98.83%), Postives = 767/772 (99.35%), Query Frame = 0

Query: 1   MANFHRCSSVFFFFF-LCFLPVIFSRSSDKQETFIVHVSRSEKPSLFSSHYRWHSSILDS 60
           MANFHRCSSVFFFFF LCFLPVIFSRS DKQETFIVHVS+ EKPSLFSSHYRWHSSILDS
Sbjct: 1   MANFHRCSSVFFFFFFLCFLPVIFSRSPDKQETFIVHVSKLEKPSLFSSHYRWHSSILDS 60

Query: 61  LSPSSRPRKLLYNYERAANGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFLG 120
           LSPSSRPRKLLYNYERA NGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFLG
Sbjct: 61  LSPSSRPRKLLYNYERAVNGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFLG 120

Query: 121 LADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATTC 180
           LADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATTC
Sbjct: 121 LADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATTC 180

Query: 181 NRKIIGARAFFHGYESNIRRPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYAR 240
           NRKIIGARAFFHGYESNI RPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYAR
Sbjct: 181 NRKIIGARAFFHGYESNIGRPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYAR 240

Query: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300
           GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300

Query: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
           IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF
Sbjct: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360

Query: 361 SGVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAV 420
           SGVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAV
Sbjct: 361 SGVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAV 420

Query: 421 KSAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTV 480
           KSAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGT+
Sbjct: 421 KSAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTI 480

Query: 481 ISDSPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFN 540
           ISDSPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFN
Sbjct: 481 ISDSPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSNP 600
           IISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSNP 600

Query: 601 FVHGGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSN 660
           FVHGGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSN
Sbjct: 601 FVHGGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSN 660

Query: 661 PGNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDKADAVYEVKVEAPQGIEISVLPNKLEFS 720
           PGNLNYPSFAVVFTSNGEEVVKYTRTVTNVGD+ADAVYEVKVEAPQG+EISVLPNKLEFS
Sbjct: 661 PGNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDEADAVYEVKVEAPQGVEISVLPNKLEFS 720

Query: 721 AKKTTQSYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM 771
           AKKTTQSYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM
Sbjct: 721 AKKTTQSYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM 772

BLAST of Cp4.1LG12g09230.1 vs. NCBI nr
Match: KAG6575439.1 (Subtilisin-like protease 1.4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1519 bits (3934), Expect = 0.0
Identity = 763/773 (98.71%), Postives = 766/773 (99.09%), Query Frame = 0

Query: 1   MANFHRCSSVFFFFF--LCFLPVIFSRSSDKQETFIVHVSRSEKPSLFSSHYRWHSSILD 60
           MANFHRCSSVFFFFF  LCFLPVIFSRS DKQETFIVHVS+ EKPSLFSSHYRWHSSILD
Sbjct: 1   MANFHRCSSVFFFFFFFLCFLPVIFSRSPDKQETFIVHVSKLEKPSLFSSHYRWHSSILD 60

Query: 61  SLSPSSRPRKLLYNYERAANGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFL 120
           SLSPSSRPRKLLYNYERA NGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFL
Sbjct: 61  SLSPSSRPRKLLYNYERAVNGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFL 120

Query: 121 GLADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATT 180
           GLADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATT
Sbjct: 121 GLADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATT 180

Query: 181 CNRKIIGARAFFHGYESNIRRPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYA 240
           CNRKIIGARAFFHGYESNI RPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYA
Sbjct: 181 CNRKIIGARAFFHGYESNIGRPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYA 240

Query: 241 RGEARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRD 300
           RGEARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRD
Sbjct: 241 RGEARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRD 300

Query: 301 SIAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRV 360
           SIAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRV
Sbjct: 301 SIAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRV 360

Query: 361 FSGVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSA 420
           FSGVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSA
Sbjct: 361 FSGVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSA 420

Query: 421 VKSAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGT 480
           VKSAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGT
Sbjct: 421 VKSAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGT 480

Query: 481 VISDSPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEF 540
           VISDSPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEF
Sbjct: 481 VISDSPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEF 540

Query: 541 NIISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSN 600
           NIISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSN
Sbjct: 541 NIISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSN 600

Query: 601 PFVHGGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLS 660
           PFVHGGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLS
Sbjct: 601 PFVHGGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLS 660

Query: 661 NPGNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDKADAVYEVKVEAPQGIEISVLPNKLEF 720
           NPGNLNYPSFAVVFTSNGEEVVKYTRTVTNVGD+ADAVYEVKVEAPQG+EISVLPNKLEF
Sbjct: 661 NPGNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDEADAVYEVKVEAPQGVEISVLPNKLEF 720

Query: 721 SAKKTTQSYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM 771
           SAKKTTQSYEITFTKISGFKNSASFGSIQWSDGSH VRSPIAVSFNTASIASM
Sbjct: 721 SAKKTTQSYEITFTKISGFKNSASFGSIQWSDGSHTVRSPIAVSFNTASIASM 773

BLAST of Cp4.1LG12g09230.1 vs. NCBI nr
Match: XP_022992130.1 (subtilisin-like protease SBT1.4 [Cucurbita maxima])

HSP 1 Score: 1508 bits (3903), Expect = 0.0
Identity = 755/771 (97.92%), Postives = 760/771 (98.57%), Query Frame = 0

Query: 1   MANFHRCSSVFFFFFLCFLPVIFSRSSDKQETFIVHVSRSEKPSLFSSHYRWHSSILDSL 60
           MANFHRCSSVFFFFFLCFLPVIFSRS DKQETFIVHVS+SEKPSLFSSHYRWHSSILDSL
Sbjct: 1   MANFHRCSSVFFFFFLCFLPVIFSRSPDKQETFIVHVSKSEKPSLFSSHYRWHSSILDSL 60

Query: 61  SPSSRPRKLLYNYERAANGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFLGL 120
           SPSSRPRKLLYNYERA NGFAARIT  QAAELRRVPGVV VIPDRMRQLHTTRTPHFLGL
Sbjct: 61  SPSSRPRKLLYNYERAVNGFAARITADQAAELRRVPGVVLVIPDRMRQLHTTRTPHFLGL 120

Query: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATTCN 180
           ADNLGLWPDTNYADDVIIGVLDTGIWPE PSFSDEGLSPVPASWKGTCEAGEGFSATTCN
Sbjct: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPEHPSFSDEGLSPVPASWKGTCEAGEGFSATTCN 180

Query: 181 RKIIGARAFFHGYESNIRRPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARG 240
           RKIIGARAFFHGYESNI R LHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARG
Sbjct: 181 RKIIGARAFFHGYESNIGRRLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARG 240

Query: 241 EARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
           EARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI
Sbjct: 241 EARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300

Query: 301 AIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS 360
           AIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS
Sbjct: 301 AIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS 360

Query: 361 GVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAVK 420
           GVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAVK
Sbjct: 361 GVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAVK 420

Query: 421 SAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTVI 480
           SAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTVI
Sbjct: 421 SAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTVI 480

Query: 481 SDSPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFNI 540
            DSPP+PRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSNPF 600
           ISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSG PIKDLSTSNDSNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGSPIKDLSTSNDSNPF 600

Query: 601 VHGGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSNP 660
           VHGGGHIDPNKALNPGLIYDLNP DYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSNP
Sbjct: 601 VHGGGHIDPNKALNPGLIYDLNPHDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSNP 660

Query: 661 GNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDKADAVYEVKVEAPQGIEISVLPNKLEFSA 720
           GNLNYPSFAVVFTSNGEEVVKYTRTVTNVGD+ADAVYEVKVEAPQG+EISVLPNKLEFSA
Sbjct: 661 GNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDEADAVYEVKVEAPQGVEISVLPNKLEFSA 720

Query: 721 KKTTQSYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM 771
           KKTT+SYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM
Sbjct: 721 KKTTESYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM 771

BLAST of Cp4.1LG12g09230.1 vs. NCBI nr
Match: XP_038897980.1 (subtilisin-like protease SBT1.4 [Benincasa hispida])

HSP 1 Score: 1388 bits (3592), Expect = 0.0
Identity = 687/771 (89.11%), Postives = 729/771 (94.55%), Query Frame = 0

Query: 1   MANFHRCSSVFFFFFLCFLPVIFSRSSDKQETFIVHVSRSEKPSLFSSHYRWHSSILDSL 60
           MA  H CSS+FF F LCF PVIFSRS + QETFIVHVS+SEKPSLFSSH+ WHSSIL+SL
Sbjct: 1   MAKSHLCSSLFFLFILCFAPVIFSRSPENQETFIVHVSKSEKPSLFSSHHHWHSSILESL 60

Query: 61  SPSSRPRKLLYNYERAANGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFLGL 120
           SPS  P KLLYNYERA NGF+AR+T AQAA+LR VPGV+SVIPDR+RQLHTTRTPHFLGL
Sbjct: 61  SPSPHPTKLLYNYERAVNGFSARLTAAQAAQLRHVPGVISVIPDRIRQLHTTRTPHFLGL 120

Query: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATTCN 180
           ADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCE GEGFSA+ CN
Sbjct: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASFCN 180

Query: 181 RKIIGARAFFHGYESNIRRPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARG 240
           RKIIGARA+F+GY SN  R  +G SDFKSARD++GHGTHTASTAAGSFVKNASFFQYARG
Sbjct: 181 RKIIGARAYFYGYASNFGRSFNGSSDFKSARDTEGHGTHTASTAAGSFVKNASFFQYARG 240

Query: 241 EARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
           EARGMASRARIAAYKICWEFGC+DSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI
Sbjct: 241 EARGMASRARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300

Query: 301 AIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS 360
           AIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS
Sbjct: 301 AIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS 360

Query: 361 GVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAVK 420
           GVSLYSGDPLGDSK+PLVYGGDCG+RYCYSGSL+PSKVAGKIVVCDRGGNARVAKG AVK
Sbjct: 361 GVSLYSGDPLGDSKVPLVYGGDCGSRYCYSGSLNPSKVAGKIVVCDRGGNARVAKGGAVK 420

Query: 421 SAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTVI 480
           SAGGLG++LANTEENGEELLADSHLIPGTM+GEIAGNKLRDYIHSDPNP+A IVFRGTVI
Sbjct: 421 SAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHSDPNPTAIIVFRGTVI 480

Query: 481 SDSPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFNI 540
            DSPP+PRVAAFSSRGPNYRTAEILKPDVIAPGVNILA WSGY+SP+ L+ID RRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILASWSGYSSPTGLNIDPRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSNPF 600
           ISGTSMSCPHVSGVAALLRKAFPTW+PAAIKSALITTSYSLD+SG PIKDLSTS +SNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSEESNPF 600

Query: 601 VHGGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSNP 660
           VHG GHIDPNKALNPGLIYD++PQDYVSFLCSIGYDS+QIAVFVKDSSYS LCEHKLSNP
Sbjct: 601 VHGAGHIDPNKALNPGLIYDISPQDYVSFLCSIGYDSKQIAVFVKDSSYSQLCEHKLSNP 660

Query: 661 GNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDKADAVYEVKVEAPQGIEISVLPNKLEFSA 720
           GNLNYPSFAVVF  NGE+VVKYTRTVTNVGD++D VYEVKVEAPQG+EISV+PNKL F+A
Sbjct: 661 GNLNYPSFAVVF--NGEDVVKYTRTVTNVGDESDVVYEVKVEAPQGVEISVVPNKLSFNA 720

Query: 721 KKTTQSYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM 771
           +KTT SYEITFTKISGF  +ASFGSIQWSDGSH VRSPIAVSFNT SIASM
Sbjct: 721 EKTTDSYEITFTKISGFVEAASFGSIQWSDGSHNVRSPIAVSFNTGSIASM 769

BLAST of Cp4.1LG12g09230.1 vs. ExPASy TrEMBL
Match: A0A6J1GQK5 (subtilisin-like protease SBT1.4 OS=Cucurbita moschata OX=3662 GN=LOC111456188 PE=3 SV=1)

HSP 1 Score: 1521 bits (3939), Expect = 0.0
Identity = 763/772 (98.83%), Postives = 767/772 (99.35%), Query Frame = 0

Query: 1   MANFHRCSSVFFFFF-LCFLPVIFSRSSDKQETFIVHVSRSEKPSLFSSHYRWHSSILDS 60
           MANFHRCSSVFFFFF LCFLPVIFSRS DKQETFIVHVS+ EKPSLFSSHYRWHSSILDS
Sbjct: 1   MANFHRCSSVFFFFFFLCFLPVIFSRSPDKQETFIVHVSKLEKPSLFSSHYRWHSSILDS 60

Query: 61  LSPSSRPRKLLYNYERAANGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFLG 120
           LSPSSRPRKLLYNYERA NGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFLG
Sbjct: 61  LSPSSRPRKLLYNYERAVNGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFLG 120

Query: 121 LADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATTC 180
           LADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATTC
Sbjct: 121 LADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATTC 180

Query: 181 NRKIIGARAFFHGYESNIRRPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYAR 240
           NRKIIGARAFFHGYESNI RPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYAR
Sbjct: 181 NRKIIGARAFFHGYESNIGRPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYAR 240

Query: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300
           GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300

Query: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
           IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF
Sbjct: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360

Query: 361 SGVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAV 420
           SGVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAV
Sbjct: 361 SGVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAV 420

Query: 421 KSAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTV 480
           KSAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGT+
Sbjct: 421 KSAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTI 480

Query: 481 ISDSPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFN 540
           ISDSPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFN
Sbjct: 481 ISDSPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSNP 600
           IISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSNP 600

Query: 601 FVHGGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSN 660
           FVHGGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSN
Sbjct: 601 FVHGGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSN 660

Query: 661 PGNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDKADAVYEVKVEAPQGIEISVLPNKLEFS 720
           PGNLNYPSFAVVFTSNGEEVVKYTRTVTNVGD+ADAVYEVKVEAPQG+EISVLPNKLEFS
Sbjct: 661 PGNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDEADAVYEVKVEAPQGVEISVLPNKLEFS 720

Query: 721 AKKTTQSYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM 771
           AKKTTQSYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM
Sbjct: 721 AKKTTQSYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM 772

BLAST of Cp4.1LG12g09230.1 vs. ExPASy TrEMBL
Match: A0A6J1JUV1 (subtilisin-like protease SBT1.4 OS=Cucurbita maxima OX=3661 GN=LOC111488562 PE=3 SV=1)

HSP 1 Score: 1508 bits (3903), Expect = 0.0
Identity = 755/771 (97.92%), Postives = 760/771 (98.57%), Query Frame = 0

Query: 1   MANFHRCSSVFFFFFLCFLPVIFSRSSDKQETFIVHVSRSEKPSLFSSHYRWHSSILDSL 60
           MANFHRCSSVFFFFFLCFLPVIFSRS DKQETFIVHVS+SEKPSLFSSHYRWHSSILDSL
Sbjct: 1   MANFHRCSSVFFFFFLCFLPVIFSRSPDKQETFIVHVSKSEKPSLFSSHYRWHSSILDSL 60

Query: 61  SPSSRPRKLLYNYERAANGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFLGL 120
           SPSSRPRKLLYNYERA NGFAARIT  QAAELRRVPGVV VIPDRMRQLHTTRTPHFLGL
Sbjct: 61  SPSSRPRKLLYNYERAVNGFAARITADQAAELRRVPGVVLVIPDRMRQLHTTRTPHFLGL 120

Query: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATTCN 180
           ADNLGLWPDTNYADDVIIGVLDTGIWPE PSFSDEGLSPVPASWKGTCEAGEGFSATTCN
Sbjct: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPEHPSFSDEGLSPVPASWKGTCEAGEGFSATTCN 180

Query: 181 RKIIGARAFFHGYESNIRRPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARG 240
           RKIIGARAFFHGYESNI R LHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARG
Sbjct: 181 RKIIGARAFFHGYESNIGRRLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARG 240

Query: 241 EARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
           EARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI
Sbjct: 241 EARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300

Query: 301 AIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS 360
           AIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS
Sbjct: 301 AIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS 360

Query: 361 GVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAVK 420
           GVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAVK
Sbjct: 361 GVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAVK 420

Query: 421 SAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTVI 480
           SAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTVI
Sbjct: 421 SAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTVI 480

Query: 481 SDSPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFNI 540
            DSPP+PRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSNPF 600
           ISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSG PIKDLSTSNDSNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGSPIKDLSTSNDSNPF 600

Query: 601 VHGGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSNP 660
           VHGGGHIDPNKALNPGLIYDLNP DYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSNP
Sbjct: 601 VHGGGHIDPNKALNPGLIYDLNPHDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSNP 660

Query: 661 GNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDKADAVYEVKVEAPQGIEISVLPNKLEFSA 720
           GNLNYPSFAVVFTSNGEEVVKYTRTVTNVGD+ADAVYEVKVEAPQG+EISVLPNKLEFSA
Sbjct: 661 GNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDEADAVYEVKVEAPQGVEISVLPNKLEFSA 720

Query: 721 KKTTQSYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM 771
           KKTT+SYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM
Sbjct: 721 KKTTESYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM 771

BLAST of Cp4.1LG12g09230.1 vs. ExPASy TrEMBL
Match: A0A6J1KIR1 (subtilisin-like protease SBT1.4 OS=Cucurbita maxima OX=3661 GN=LOC111494328 PE=3 SV=1)

HSP 1 Score: 1381 bits (3574), Expect = 0.0
Identity = 681/771 (88.33%), Postives = 727/771 (94.29%), Query Frame = 0

Query: 1   MANFHRCSSVFFFFFLCFLPVIFSRSSDKQETFIVHVSRSEKPSLFSSHYRWHSSILDSL 60
           MAN H CSSVFFF  LCF+PVIFSRS   QETFIVHVS+SEKP+LFSSH+ WHSSILDSL
Sbjct: 1   MANLHLCSSVFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILDSL 60

Query: 61  SPSSRPRKLLYNYERAANGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFLGL 120
           SPS  P KLLYNYE AANGF+ARIT AQAAELRR+PGV+SVIPDR+RQLHTTRTPHFLGL
Sbjct: 61  SPSPHPTKLLYNYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL 120

Query: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATTCN 180
           AD+ GLW +TNYADDVIIGVLDTGIWPERPSFSDEGLSPVPA+WKGTC+ G GFSA+ CN
Sbjct: 121 ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN 180

Query: 181 RKIIGARAFFHGYESNIRRPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARG 240
           RKIIGARA+F GYES +RR L G SDFKSARD+DGHGTHTASTAAGSFVKNASFFQYARG
Sbjct: 181 RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240

Query: 241 EARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
           EARGMA+RARIAAYKICWE GCYDSDILAAMDQAIADGVHIISLSVGSSGR PAYYRDSI
Sbjct: 241 EARGMANRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI 300

Query: 301 AIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS 360
           AIGAFGAMQHG+VVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGDGRVFS
Sbjct: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS 360

Query: 361 GVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAVK 420
           GVSLYSGDPLGDSKLPLVY GDCGNRYCYSG+LD SKVAGKIVVCDRGGNARVAKGS VK
Sbjct: 361 GVSLYSGDPLGDSKLPLVYAGDCGNRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSTVK 420

Query: 421 SAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTVI 480
           +AGGLG+ILANTEENGEELLADSHLIPGTMIGEIAGN L+DYIHSDP+P+ATIVFRGTVI
Sbjct: 421 TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI 480

Query: 481 SDSPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFNI 540
            DSPP+PRVAAFSSRGPN+RTAEILKPDVIAPGVNILAGWSGY+SP+SLSID RRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNFRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSNPF 600
           ISGTSMSCPHVSGVAALLRKAFPTW+PAAIKSAL+TTSYSLD+SGRPI DLSTS  SNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF 600

Query: 601 VHGGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSNP 660
           VHG GHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDS Y+ LC+HKL++P
Sbjct: 601 VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAELCKHKLTSP 660

Query: 661 GNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDKADAVYEVKVEAPQGIEISVLPNKLEFSA 720
           GNLNYPSFAVVF S+G+EVVKYTRTVTNVGD+ DAVYEVKVE+P+G+EI+VLPNKLEFSA
Sbjct: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPEGVEITVLPNKLEFSA 720

Query: 721 KKTTQSYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM 771
           K  TQSYEITFTKI+G KNSASFGSIQWSDGSHIVRSP+AVSFN+  IASM
Sbjct: 721 KNRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771

BLAST of Cp4.1LG12g09230.1 vs. ExPASy TrEMBL
Match: A0A6J1HMN7 (subtilisin-like protease SBT1.4 OS=Cucurbita moschata OX=3662 GN=LOC111464338 PE=3 SV=1)

HSP 1 Score: 1379 bits (3570), Expect = 0.0
Identity = 682/771 (88.46%), Postives = 727/771 (94.29%), Query Frame = 0

Query: 1   MANFHRCSSVFFFFFLCFLPVIFSRSSDKQETFIVHVSRSEKPSLFSSHYRWHSSILDSL 60
           MAN H CSS FFF  LCF+PVIFSRS   QETFIVHVS+SEKP+LFSSH+ WHSSIL+SL
Sbjct: 1   MANLHLCSSGFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILESL 60

Query: 61  SPSSRPRKLLYNYERAANGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFLGL 120
           SPS  P KLLY+YE AANGF+ARIT AQAAELRR+PGV+SVIPDR+RQLHTTRTPHFLGL
Sbjct: 61  SPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL 120

Query: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATTCN 180
           AD+ GLW +TNYADDVIIGVLDTGIWPERPSFSDEGLSPVPA+WKGTC+ G GFSA+ CN
Sbjct: 121 ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN 180

Query: 181 RKIIGARAFFHGYESNIRRPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARG 240
           RKIIGARA+F GYES +RR L G SDFKSARD+DGHGTHTASTAAGSFVKNASFFQYARG
Sbjct: 181 RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240

Query: 241 EARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
           EARGMASRARIAAYKICWE GCYDSDILAAMDQAIADGVHIISLSVGSSGR PAYYRDSI
Sbjct: 241 EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI 300

Query: 301 AIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS 360
           AIGAFGAMQHG+VVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGDGRVFS
Sbjct: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS 360

Query: 361 GVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAVK 420
           GVSLYSGDPLGDSKLPLVY GDCG+RYCYSG+LD SKVAGKIVVCDRGGNARVAKGSAVK
Sbjct: 361 GVSLYSGDPLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK 420

Query: 421 SAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTVI 480
           +AGGLG+ILANTEENGEELLADSHLIPGTMIGEIAGN L+DYIHSDP+P+ATIVFRGTVI
Sbjct: 421 TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI 480

Query: 481 SDSPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFNI 540
            DSPP+PRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGY+SP+SLSID RRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSNPF 600
           ISGTSMSCPHVSGVAALLRKAFPTW+PAAIKSAL+TTSYSLD+SGRPI DLSTS  SNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF 600

Query: 601 VHGGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSNP 660
           VHG GHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDS Y+ LCEHKL++P
Sbjct: 601 VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTSP 660

Query: 661 GNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDKADAVYEVKVEAPQGIEISVLPNKLEFSA 720
           GNLNYPSFAVVF S+G+EVVKYTRTVTNVGD+ DAVYEVKVE+PQG+EI+VLPNKLEFSA
Sbjct: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFSA 720

Query: 721 KKTTQSYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM 771
           K  TQSYEITFTKI+G KNSASFGSIQWSDGSHIVRSP+AVSFN+  IASM
Sbjct: 721 KDRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771

BLAST of Cp4.1LG12g09230.1 vs. ExPASy TrEMBL
Match: A0A1S3CGN8 (LOW QUALITY PROTEIN: subtilisin-like protease SBT1.4 OS=Cucumis melo OX=3656 GN=LOC103500685 PE=3 SV=1)

HSP 1 Score: 1352 bits (3499), Expect = 0.0
Identity = 673/772 (87.18%), Postives = 720/772 (93.26%), Query Frame = 0

Query: 1   MANFHRCSSVFFFFFLCFL-PVIFSRSSDKQETFIVHVSRSEKPSLFSSHYRWHSSILDS 60
           MANFH  SS FFFFF+ F+ P++FSRS + QET+IVHVS+SEKPSLFSSH+ WHSSIL+S
Sbjct: 1   MANFHLPSSSFFFFFVLFVFPLVFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILES 60

Query: 61  LSPSSRPRKLLYNYERAANGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFLG 120
           LSPS  P KLLYNYERAANGF+ARIT AQA ELRRVPG++SVIPDR+RQLHTTRTPHFLG
Sbjct: 61  LSPSPHPTKLLYNYERAANGFSARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLG 120

Query: 121 LADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATTC 180
           LADNLGLW DTNYADDVIIGVLDTGIWPERPSFSDEGL+PVPA WKGTC+ GEG SA  C
Sbjct: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALAC 180

Query: 181 NRKIIGARAFFHGYESNIRRPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYAR 240
           NRKIIGARAFF GYESN+R  L G SDFKSARD+DGHGTHTASTAAGSFVKNASFFQYAR
Sbjct: 181 NRKIIGARAFFXGYESNLRGSLKGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240

Query: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300
           GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAI+DGV +ISLSVGSSGRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300

Query: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
           IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGD RVF
Sbjct: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVF 360

Query: 361 SGVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAV 420
           SGVSLYSGDPLGDSKLPLVYGGDCG+RYCYSGSLD SKVAG+IVVCDRGGNARVAKG AV
Sbjct: 361 SGVSLYSGDPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGRIVVCDRGGNARVAKGGAV 420

Query: 421 KSAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTV 480
           KSAGGLG++LANTEENGEELLADSHLIPGTM+GEIAGNKLRDYIH+DPNP+ATIVFRGTV
Sbjct: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTV 480

Query: 481 ISDSPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFN 540
           I DSPP+P+VA+FSSRGPNYRTAEILKPDVIAPGVNILAGWSGY+SP+ L+ID RRVEFN
Sbjct: 481 IGDSPPAPKVASFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSNP 600
           IISGTSMSCPHVSGVAALLRKAFPTW+PAAIKSALITTSYSLD+SG PIKDL+TS +SNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNP 600

Query: 601 FVHGGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSN 660
           FVHG GHIDPN+ALNPGLIYDL PQDYVSFLCSIGYDS+QIAVFVK SSYS LCEHKLSN
Sbjct: 601 FVHGAGHIDPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSN 660

Query: 661 PGNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDKADAVYEVKVEAPQGIEISVLPNKLEFS 720
           PGNLNYPSF+VVF     EVVKYTRTVTNVGD+ + VY VKVEAPQG+EISV+PNKLEF+
Sbjct: 661 PGNLNYPSFSVVFDDG--EVVKYTRTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFN 720

Query: 721 AKKTTQSYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM 771
            +KTT SYEITFTKI+GFK SASFGSIQWSDG H VRSPIAVSF T SIASM
Sbjct: 721 KEKTTLSYEITFTKINGFKESASFGSIQWSDGIHNVRSPIAVSFKTGSIASM 770

BLAST of Cp4.1LG12g09230.1 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 981.9 bits (2537), Expect = 3.0e-286
Identity = 492/772 (63.73%), Postives = 594/772 (76.94%), Query Frame = 0

Query: 8   SSVFFFF--FLCFLPVIFSRSSDKQETFIVHVSRSEKPSLFSSHYRWHSSILDSLSPSSR 67
           SS+FF F   LCF     S SSD  E++IVHV RS KPSLFSSH  WH S+L SL  S +
Sbjct: 7   SSIFFVFPLLLCFFSP-SSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQ 66

Query: 68  PRKLLYNYERAANGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFLGLADNLG 127
           P  LLY+Y RA +GF+AR++  Q A LRR P V+SVIPD+ R++HTT TP FLG + N G
Sbjct: 67  PATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSG 126

Query: 128 LWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATTCNRKIIG 187
           LW ++NY +DVI+GVLDTGIWPE PSFSD GL P+P++WKG CE G  F A++CNRK+IG
Sbjct: 127 LWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIG 186

Query: 188 ARAFFHGYESNIR-RPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARGEARG 247
           ARAF+ GY +       H   + +S RD++GHGTHTASTAAGS V NAS +QYARG A G
Sbjct: 187 ARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATG 246

Query: 248 MASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGA 307
           MAS+ARIAAYKICW  GCYDSDILAAMDQA+ADGVH+ISLSVG+SG AP Y+ DSIAIGA
Sbjct: 247 MASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGA 306

Query: 308 FGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSL 367
           FGA +HGIVVSCSAGNSGP P TA NIAPWILTVGAST+DREF A+ I GDG+VF+G SL
Sbjct: 307 FGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSL 366

Query: 368 YSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAVKSAGG 427
           Y+G+ L DS+L LVY GDCG+R CY G L+ S V GKIV+CDRGGNARV KGSAVK AGG
Sbjct: 367 YAGESLPDSQLSLVYSGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKLAGG 426

Query: 428 LGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTVISDSP 487
            G+ILANT E+GEEL ADSHL+P TM+G  AG+++RDYI +  +P+A I F GT+I  SP
Sbjct: 427 AGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSP 486

Query: 488 PSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFNIISGT 547
           PSPRVAAFSSRGPN+ T  ILKPDVIAPGVNILAGW+G   P+ L ID RRV+FNIISGT
Sbjct: 487 PSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIISGT 546

Query: 548 SMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSNPFVHGG 607
           SMSCPHVSG+AALLRKA P W+PAAIKSAL+TT+Y ++ SG PI+DL+T   SN F+HG 
Sbjct: 547 SMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGA 606

Query: 608 GHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCE-HKLSNPGNL 667
           GH+DPNKALNPGL+YD+  ++YV+FLC++GY+   I VF++D +  + CE  KL   G+L
Sbjct: 607 GHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDL 666

Query: 668 NYPSFAVVFTSNGEEVVKYTRTVTNVGDKADAVYEVKVEAPQGIEISVLPNKLEFSAKKT 727
           NYPSF+VVF S G EVVKY R V NVG   DAVYEV V++P  +EI V P+KL FS +K+
Sbjct: 667 NYPSFSVVFASTG-EVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKS 726

Query: 728 TQSYEITFTKI-----SGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIAS 771
              YE+TF  +      G      FGSI+W+DG H+V+SP+AV +   S+ S
Sbjct: 727 VLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQWGQGSVQS 776

BLAST of Cp4.1LG12g09230.1 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 777.7 bits (2007), Expect = 8.6e-225
Identity = 415/764 (54.32%), Postives = 530/764 (69.37%), Query Frame = 0

Query: 8   SSVFFFFFLCFLPVIFSRSSDKQETFIVHVSRSEKPSLFSSHYRWHSSILDSLSPSSRPR 67
           SS  FF  LC      S SS  Q T+IVH+++S+ PS F  H  W+ S L S+S S+   
Sbjct: 7   SSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSA--- 66

Query: 68  KLLYNYERAANGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFLGLADNLG-L 127
           +LLY YE A +GF+ R+T  +A  L   PGV+SV+P+   +LHTTRTP FLGL ++   L
Sbjct: 67  ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL 126

Query: 128 WPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATTCNRKIIGA 187
           +P+     DV++GVLDTG+WPE  S+SDEG  P+P+SWKG CEAG  F+A+ CNRK+IGA
Sbjct: 127 FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGA 186

Query: 188 RAFFHGYESNIRRPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARGEARGMA 247
           R F  GYES +  P+    + +S RD DGHGTHT+STAAGS V+ AS   YA G ARGMA
Sbjct: 187 RFFARGYESTM-GPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMA 246

Query: 248 SRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFG 307
            RAR+A YK+CW  GC+ SDILAA+D+AIAD V+++S+S+G  G    YYRD +AIGAF 
Sbjct: 247 PRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLG--GGMSDYYRDGVAIGAFA 306

Query: 308 AMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYS 367
           AM+ GI+VSCSAGN+GP   +  N+APWI TVGA T+DR+F A  ILG+G+ F+GVSL+ 
Sbjct: 307 AMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFK 366

Query: 368 GDPLGDSKLPLVYGGDCGN----RYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAVKSA 427
           G+ L D  LP +Y G+  N      C +G+L P KV GKIV+CDRG NARV KG  VK+A
Sbjct: 367 GEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAA 426

Query: 428 GGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTVISD 487
           GG+G+ILANT  NGEEL+AD+HL+P T +GE AG+ +R Y+ +DPNP+A+I   GTV+  
Sbjct: 427 GGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVG- 486

Query: 488 SPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFNIIS 547
             PSP VAAFSSRGPN  T  ILKPD+IAPGVNILA W+G   P+ L+ D+RRVEFNIIS
Sbjct: 487 VKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIIS 546

Query: 548 GTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSNPFVH 607
           GTSMSCPHVSG+AALL+   P W+PAAI+SAL+TT+Y     G+P+ D++T   S PF H
Sbjct: 547 GTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDH 606

Query: 608 GGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCE-HKLSNPG 667
           G GH+ P  A NPGLIYDL  +DY+ FLC++ Y S QI   V   +Y+  C+  K  +  
Sbjct: 607 GAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIR-SVSRRNYT--CDPSKSYSVA 666

Query: 668 NLNYPSFAVVFTSNGEEVVKYTRTVTNVGDKADAVYEVKVEA-PQGIEISVLPNKLEFSA 727
           +LNYPSFAV    +G    KYTRTVT+VG      Y VKV +   G++ISV P  L F  
Sbjct: 667 DLNYPSFAV--NVDGVGAYKYTRTVTSVG--GAGTYSVKVTSETTGVKISVEPAVLNFKE 726

Query: 728 KKTTQSYEITFTKISGF-KNSASFGSIQWSDGSHIVRSPIAVSF 764
               +SY +TFT  S     S SFGSI+WSDG H+V SP+A+S+
Sbjct: 727 ANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of Cp4.1LG12g09230.1 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 743.4 bits (1918), Expect = 1.8e-214
Identity = 388/740 (52.43%), Postives = 508/740 (68.65%), Query Frame = 0

Query: 30  QETFIVHVSRSEKPSLFSSHYRWHSSILDSLSPSSRPRKLLYNYERAANGFAARITDAQA 89
           ++T+I+ V+ S+KP  F +H+ W++S L+S S       LLY Y  + +GF+A +   +A
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES------SLLYTYTTSFHGFSAYLDSTEA 86

Query: 90  -AELRRVPGVVSVIPDRMRQLHTTRTPHFLGLADNLGLWPDTNYADDVIIGVLDTGIWPE 149
            + L     ++ +  D +  LHTTRTP FLGL    G+    + ++ VIIGVLDTG+WPE
Sbjct: 87  DSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPE 146

Query: 150 RPSFSDEGLSPVPASWKGTCEAGEGFSATTCNRKIIGARAFFHGYESNIRRPLHGLSDFK 209
             SF D  +  +P+ WKG CE+G  F +  CN+K+IGAR+F  G++           +  
Sbjct: 147 SRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESV 206

Query: 210 SARDSDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRARIAAYKICWEFGCYDSDIL 269
           S RD DGHGTHT++TAAGS V+NASF  YA G ARGMA+RAR+A YK+CW  GC+ SDIL
Sbjct: 207 SPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDIL 266

Query: 270 AAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGAMQHGIVVSCSAGNSGPGPYTA 329
           AAMD+AI DGV ++SLS+G  G AP YYRD+IAIGAF AM+ G+ VSCSAGNSGP   + 
Sbjct: 267 AAMDRAILDGVDVLSLSLG-GGSAP-YYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASV 326

Query: 330 VNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSGDPLGDSKLPLVY--GGDCGNR 389
            N+APW++TVGA T+DR+F A   LG+G+  +GVSLYSG  +G   L LVY  G    + 
Sbjct: 327 ANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGNSSSSN 386

Query: 390 YCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAVKSAGGLGVILANTEENGEELLADSHLI 449
            C  GSLD S V GKIVVCDRG NARV KG+ V+ AGGLG+I+ANT  +GEEL+ADSHL+
Sbjct: 387 LCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLL 446

Query: 450 PGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTVISDSPPSPRVAAFSSRGPNYRTAEILK 509
           P   +G+  G+ LR+Y+ SD  P+A +VF+GTV+ D  PSP VAAFSSRGPN  T EILK
Sbjct: 447 PAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVL-DVKPSPVVAAFSSRGPNTVTPEILK 506

Query: 510 PDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFNIISGTSMSCPHVSGVAALLRKAFPTWT 569
           PDVI PGVNILAGWS    P+ L  D+RR +FNI+SGTSMSCPH+SG+A LL+ A P W+
Sbjct: 507 PDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWS 566

Query: 570 PAAIKSALITTSYSLDTSGRPIKDLSTSNDSNPFVHGGGHIDPNKALNPGLIYDLNPQDY 629
           P+AIKSAL+TT+Y LD +  P+ D + ++ SNP+ HG GH+DP KAL+PGL+YD++ ++Y
Sbjct: 567 PSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEY 626

Query: 630 VSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSNPGNLNYPSFAVVFTSNGEEVVKYTRTV 689
           + FLCS+ Y    I   VK  S +  C  K S+PG LNYPSF+V+F   G+ VV+YTR V
Sbjct: 627 IRFLCSLDYTVDHIVAIVKRPSVN--CSKKFSDPGQLNYPSFSVLF--GGKRVVRYTREV 686

Query: 690 TNVGDKADAVYEVKVEAPQGIEISVLPNKLEFSAKKTTQSYEITFTKISG--FKNSASFG 749
           TNVG  A +VY+V V     + ISV P+KL F +    + Y +TF    G    N A FG
Sbjct: 687 TNVG-AASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFG 746

Query: 750 SIQWSDGSHIVRSPIAVSFN 765
           SI WS+  H VRSP+A S+N
Sbjct: 747 SITWSNPQHEVRSPVAFSWN 752

BLAST of Cp4.1LG12g09230.1 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 729.6 bits (1882), Expect = 2.7e-210
Identity = 390/773 (50.45%), Postives = 515/773 (66.62%), Query Frame = 0

Query: 11  FFFFFLCFLPVIFSRSSDKQETFIVHVSRSEKPSLFSSHYRWHSSILDSLSPSSRPRKLL 70
           +FFF L       S SS    T+IVHV    KPS+F +H+ W++S L SL  +S P  ++
Sbjct: 6   YFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASL--TSSPPSII 65

Query: 71  YNYERAANGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFLGL--ADNLGLWP 130
           + Y+   +GF+AR+T   A++L   P V+SVIP+++R LHTTR+P FLGL   D  GL  
Sbjct: 66  HTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLE 125

Query: 131 DTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATTCNRKIIGARA 190
           ++++  D++IGV+DTG+WPERPSF D GL PVP  WKG C A + F  + CNRK++GAR 
Sbjct: 126 ESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARF 185

Query: 191 FFHGYESNIRRPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARGEARGMASR 250
           F  GYE+     ++  ++F+S RDSDGHGTHTAS +AG +V  AS   YA G A GMA +
Sbjct: 186 FCGGYEAT-NGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPK 245

Query: 251 ARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGAM 310
           AR+AAYK+CW  GCYDSDILAA D A+ADGV +ISLSVG  G    YY D+IAIGAFGA+
Sbjct: 246 ARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVG--GVVVPYYLDAIAIGAFGAI 305

Query: 311 QHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSGD 370
             GI VS SAGN GPG  T  N+APW+ TVGA TIDR+F A+V LG+G++ SGVS+Y G 
Sbjct: 306 DRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGP 365

Query: 371 PLGDSKL-PLVYGGDC--GNRY----CYSGSLDPSKVAGKIVVCDRGGNARVAKGSAVKS 430
            L   ++ PLVYGG    G+ Y    C  GSLDP+ V GKIV+CDRG N+R  KG  V+ 
Sbjct: 366 GLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRK 425

Query: 431 AGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYI------HSDPNPSATIVF 490
            GGLG+I+AN   +GE L+AD H++P T +G   G+++R YI       S  +P+ATIVF
Sbjct: 426 NGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVF 485

Query: 491 RGTVISDSPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRR 550
           +GT +    P+P VA+FS+RGPN  T EILKPDVIAPG+NILA W     PS ++ D RR
Sbjct: 486 KGTRLG-IRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRR 545

Query: 551 VEFNIISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSN 610
            EFNI+SGTSM+CPHVSG+AALL+ A P W+PAAI+SALITT+Y++D SG P+ D ST N
Sbjct: 546 TEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGN 605

Query: 611 DSNPFVHGGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEH 670
            S+   +G GH+ P KA++PGL+YD+   DY++FLC+  Y    I    +  +  +    
Sbjct: 606 TSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCD-GAR 665

Query: 671 KLSNPGNLNYPSFAVVFTSNGEEVVK--YTRTVTNVGDKADAVYEVKVEAPQGIEISVLP 730
           +  + GNLNYPSF+VVF   GE  +   + RTVTNVGD +D+VYE+K+  P+G  ++V P
Sbjct: 666 RAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGD-SDSVYEIKIRPPRGTTVTVEP 725

Query: 731 NKLEFSAKKTTQSYEI----TFTKISGFKNSASFGSIQWSDGSHIVRSPIAVS 763
            KL F       S+ +    T  K+S    +   G I WSDG   V SP+ V+
Sbjct: 726 EKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770

BLAST of Cp4.1LG12g09230.1 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 703.7 bits (1815), Expect = 1.6e-202
Identity = 373/767 (48.63%), Postives = 497/767 (64.80%), Query Frame = 0

Query: 8   SSVFFFFFLCFLPVIFSRSSDKQETFIVHVSRSEKPSLFSSHYRWHSSILDSLSPSSRPR 67
           S++    FL F P I   +S   +TFI  +     PS+F +HY W+S      +  +   
Sbjct: 4   STIVLLLFLSF-PFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYS------TEFAEES 63

Query: 68  KLLYNYERAANGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFLGLADNLGLW 127
           ++++ Y    +GF+A +T  +A  LR  P V++V  DR R+LHTTR+P FLGL +  GLW
Sbjct: 64  RIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLW 123

Query: 128 PDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATTCNRKIIGAR 187
            +++Y  DVIIGV DTGIWPER SFSD  L P+P  W+G CE+G  FS   CNRKIIGAR
Sbjct: 124 SESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGAR 183

Query: 188 AFFHGYESNIRRPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARGEARGMAS 247
            F  G ++ +   ++   +F S RD+DGHGTHT+STAAG     AS   YA G A+G+A 
Sbjct: 184 FFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAP 243

Query: 248 RARIAAYKICW-EFGCYDSDILAAMDQAIADGVHIISLSV-GSSGRAPAYYRDSIAIGAF 307
           +ARIAAYK+CW + GC DSDILAA D A+ DGV +IS+S+ G  G    YY D IAIG++
Sbjct: 244 KARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSY 303

Query: 308 GAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLY 367
           GA   GI VS SAGN GP   +  N+APW+ TVGASTIDR F AD ILGDG    GVSLY
Sbjct: 304 GAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLY 363

Query: 368 SGDPLGDSKLPLVYGGDCG---NRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAVKSA 427
           +G PL     P+VY G  G      C   +LDP +V GKIV+CDRG + RVAKG  VK A
Sbjct: 364 AGVPLNGRMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKA 423

Query: 428 GGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTVISD 487
           GG+G+ILAN   NGE L+ D+HLIP   +G   G++++ Y  S PNP A+I FRGT++  
Sbjct: 424 GGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVG- 483

Query: 488 SPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFNIIS 547
             P+P +A+FS RGPN  + EILKPD+IAPGVNILA W+    P+ L  D R+ EFNI+S
Sbjct: 484 IKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILS 543

Query: 548 GTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSNPFVH 607
           GTSM+CPHVSG AALL+ A P W+PA I+SA++TT+  +D S R + D ST   + P+ +
Sbjct: 544 GTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDY 603

Query: 608 GGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSNPGN 667
           G GH++  +A+NPGL+YD+   DY++FLCSIGY  + I V  +  +       +  +PGN
Sbjct: 604 GSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITR--TPVRCPTTRKPSPGN 663

Query: 668 LNYPSFAVVFTSNGEEVVKYT--RTVTNVGDKADAVYEVKVEAPQGIEISVLPNKLEFSA 727
           LNYPS   VF +N   +V  T  RT TNVG +A+AVY  ++E+P+G+ ++V P +L F++
Sbjct: 664 LNYPSITAVFPTNRRGLVSKTVIRTATNVG-QAEAVYRARIESPRGVTVTVKPPRLVFTS 723

Query: 728 KKTTQSYEITFT----KISGFKNSASFGSIQWSD-GSHIVRSPIAVS 763
               +SY +T T     +   +  A FGS+ W D G H+VRSPI V+
Sbjct: 724 AVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVT 759