Cp4.1LG10g10030.1 (mRNA) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG10g10030.1 vs. ExPASy Swiss-Prot
Match: Q9SU29 (E3 ubiquitin-protein ligase UPL5 OS=Arabidopsis thaliana OX=3702 GN=UPL5 PE=1 SV=1)

HSP 1 Score: 766.5 bits (1978), Expect = 4.1e-220
Identity = 410/840 (48.81%), Postives = 543/840 (64.64%), Query Frame = 0

Query: 81  GFDDSSTSMAAETSLKPILSPHELQFFVRTMSVGNTMVMLANTNDTVMSLHERIQSITRI 140
           G +  S S + E S         LQ FVR MS G T+V+ A   DTV  LH+RI+  T+I
Sbjct: 72  GENSRSLSSSGECSSSNRPESTRLQIFVRMMSGGKTIVIHAEKYDTVEKLHQRIEWKTKI 131

Query: 141 PVFEQRLIYRGRQLQHEQSLRECSIQNNAELQLVGRMRSTEHPKAWQIVDDMVSLVLRLY 200
           P  EQR+IY+G+QLQ E SL   SI+ +A LQLV RM+STEHP AWQ +DD++  + R+Y
Sbjct: 132 PALEQRVIYKGKQLQRENSLTYYSIEQDASLQLVARMQSTEHPVAWQTIDDIMYTISRMY 191

Query: 201 RGEVVFSASEILTTLMTDFLSLATQTDLDPAMKQLQVFLSLSAPAALVMLYLSPIKGNTD 260
           +GE +   S I   ++T F  +  ++D +   K L +F + S PAALVMLY S ++ N  
Sbjct: 192 KGENL--QSNINEKIVTFFAMIPVESD-ESIAKYLNIFSNSSVPAALVMLYASSLERNKS 251

Query: 261 CADNLIKHFMDLLCHSVPKSLKKCCAIIVLEFCNLLRRDTPEDSLYVLCRSTLGSLLETD 320
           CA + +K F+   C ++PK+ K  C  IVLEFC LLR+  P+  LYV CR+TLGS+LET 
Sbjct: 252 CAKSSVKLFLS-NCVALPKNQKNYCLPIVLEFCKLLRKVCPDQKLYVTCRNTLGSMLETF 311

Query: 321 GNSRGM-RCLESVWGPIKTPELFPFVNELANKLSIDLSSSIRSPTSAGPSXANKLSIELS 380
            N  G+       +G     E+FPF  EL   L  +L+                      
Sbjct: 312 DNPHGVYNDQYETFG----VEIFPFFTELTGLLLNELAQ--------------------- 371

Query: 381 SSIRSPTSAGPSVTDISDFTAFLLPLRNAIPEKLSFHGSKNVPLDRGGFRDSSYGEEGEF 440
                  ++GPS  D    ++F   LR  I  K++F     +P+     + ++   E   
Sbjct: 372 -------NSGPSFCDFQKVSSFWQQLRKVIELKVAFPIPIVLPM-----QSTALEAEIRH 431

Query: 441 LHNIYLSLLKQMDICLQGMEAFLTDKGKGNCVIPYIGWSQYLPILKELNDISLLFQGLKE 500
           LH ++ SLL  MD+C+  +E+ L DK  GN       WSQYL ILK +N +S ++QG K 
Sbjct: 432 LHRLFGSLLTTMDLCMCRVESSLADKEVGNSETMSSSWSQYLSILKIINSMSNIYQGAKG 491

Query: 501 EFWAIMRPRKSSICELIIRFAKRADDYSWILCHKDVINSESRRHLSMLMFPEPTEDYEEL 560
           +   ++   K S   L+++FAKR DD+ WI  +K+  N E+RRHL+ML+FP+  ED+EE+
Sbjct: 492 QLAVMLNKNKVSFSALVVKFAKRGDDHQWIFEYKEATNFEARRHLAMLLFPDVKEDFEEM 551

Query: 561 QEMLIDRSQLLEESFEYITNASVEALRHGLFVLFKNEEATGPGVLREWFLLVCKAIFNPQ 620
            EMLIDRS LL ESFEYI  AS EAL  GLF+ FKNEEATGPGVLREWF LVC+ IFNP+
Sbjct: 552 HEMLIDRSNLLSESFEYIVGASPEALHGGLFMEFKNEEATGPGVLREWFYLVCQEIFNPK 611

Query: 621 NALFVACPNDRRRFFPNPVSKVDPMHLSYFSFSGRVIALALMHNVQVGVVFDRVFFLQLA 680
           N LF+   +D RRF PNP SKVDP+H  +F F+GRVIALALMH VQVGV+FDRVFFLQLA
Sbjct: 612 NTLFLRSADDFRRFSPNPASKVDPLHPDFFEFTGRVIALALMHKVQVGVLFDRVFFLQLA 671

Query: 681 GMCISLEDIRDADPYLYGSCKQILDMDAELVDSDV-LGLTFASDFEELGTREVVDLCPGG 740
           G+ ISLEDI+D D  +Y SCKQIL+MD E  DS+  LGLTF  + EELG R+ ++LCP G
Sbjct: 672 GLKISLEDIKDTDRIMYNSCKQILEMDPEFFDSNAGLGLTFVLETEELGKRDTIELCPDG 731

Query: 741 KDMVVNSKNRKEYVQLLVENRFMKSVSKQISYFANGFTDVLADKRSHKCFFQSLELEDLD 800
           K   VNSKNRK+YV LL+E RF   + +Q+  F+ GFTD+L+     + FF+ L LEDLD
Sbjct: 732 KLKAVNSKNRKQYVDLLIERRFATPILEQVKQFSRGFTDMLSHSVPPRSFFKRLYLEDLD 791

Query: 801 WMLYGSESQISVEDWKAHTEYSGYKETDPQISWFWKIVSAMTPEQRVNLLFFWTSVKYLP 860
            ML G E+ IS++DWKAHTEY+G+KETD QI WFWKI+  MT E++ ++LFFWTS K++P
Sbjct: 792 GMLRGGENPISIDDWKAHTEYNGFKETDRQIDWFWKILKKMTEEEQRSILFFWTSNKFVP 851

Query: 861 IQGFNGLASKLYIYKSSSPYDHLPSSHTCFFRLCFPPYPSELIMKRRLEIITQEHIGCSF 919
           ++GF GL+SKLYIY+     D LP SHTCF+RLC P YP+  +M++RL +I Q+H+  SF
Sbjct: 852 VEGFRGLSSKLYIYRLYEANDRLPLSHTCFYRLCIPRYPTITLMEQRLRLIAQDHVSSSF 870

BLAST of Cp4.1LG10g10030.1 vs. ExPASy Swiss-Prot
Match: B8N7E5 (Probable E3 ubiquitin-protein ligase hulA OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=hulA PE=3 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 2.0e-54
Identity = 129/358 (36.03%), Postives = 190/358 (53.07%), Query Frame = 0

Query: 564 IDRSQLLEESFEYITNASVEALRHGLFVLFKNEEATGPGVL-REWFLLVCKAIFNPQNAL 623
           + R+ + E+S+  I   S   L+  L + F  E+    G L RE+F L+   +FNP   L
Sbjct: 462 VRRNNIFEDSYAEIMRQSASDLKKRLMIKFDGEDGLDYGGLSREFFFLLSHEMFNPFYCL 521

Query: 624 FVACPNDRRRFFPNPVSKVDPMHLSYFSFSGRVIALALMHNVQVGVVFDRVFFLQLAGMC 683
           F    +D      NP S V+P HL+YF F GRV+ LA+ H   +   F   F+  +    
Sbjct: 522 FEYSAHDNYTLQINPHSGVNPEHLNYFKFIGRVVGLAIFHRRFLDSFFIGAFYKMMLRKK 581

Query: 684 ISLEDIRDADPYLYGSCKQILDMDAELVDSDVLGLTFASDFEELGTREVVDLCPGGKDMV 743
           +SL+D+   D  L+ +    LD D E     ++ LTFA D E+ G R  +DL PGG+D+ 
Sbjct: 582 VSLQDMEGVDEDLHRNLTWTLDNDIE----GIIELTFAVDDEKFGERRTIDLKPGGRDIP 641

Query: 744 VNSKNRKEYVQLLVENRFMKSVSKQISYFANGFTDVLADKRSHKCFFQSLELEDLDWMLY 803
           V ++N+ EYV+L+ E + +K V +Q + F +GF +++     +   F   ELE    +L 
Sbjct: 642 VTNENKGEYVELVTEWKIVKRVEEQFNAFMSGFNELIPADLVN--VFDERELE----LLI 701

Query: 804 GSESQISVEDWKAHTEYSGYKETDPQISWFWKIVSAMTPEQRVNLLFFWTSVKYLPIQGF 863
           G  + I V+DWK HT+Y GY+E+D  I  FWKIV     EQ+  LL F T    +P+ GF
Sbjct: 702 GGIADIDVDDWKKHTDYRGYQESDEVIQNFWKIVRTWDAEQKSRLLQFTTGTSRIPVNGF 761

Query: 864 NGLAS-----KLYIYKSSSPYDHLPSSHTCFFRLCFPPYPSELIMKRRLEIITQEHIG 916
             L       +  I KS  P   LP SHTCF RL  PPY +  +++ +L I  +E +G
Sbjct: 762 KDLQGSDGPRRFTIEKSGDP-GALPKSHTCFNRLDLPPYKTNDVLEHKLSIAVEETLG 808

BLAST of Cp4.1LG10g10030.1 vs. ExPASy Swiss-Prot
Match: Q2UBP1 (Probable E3 ubiquitin-protein ligase hulA OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=hulA PE=3 SV=2)

HSP 1 Score: 215.7 bits (548), Expect = 2.7e-54
Identity = 129/359 (35.93%), Postives = 190/359 (52.92%), Query Frame = 0

Query: 564 IDRSQLLEESFEYITNASVEALRHGLFVLFKNEEATGPGVL--REWFLLVCKAIFNPQNA 623
           + R+ + E+S+  I   S   L+  L + F  E+    G L  RE+F L+   +FNP   
Sbjct: 465 VRRNNIFEDSYAEIMRQSASDLKKRLMIKFDGEDGLDYGGLSRREFFFLLSHEMFNPFYC 524

Query: 624 LFVACPNDRRRFFPNPVSKVDPMHLSYFSFSGRVIALALMHNVQVGVVFDRVFFLQLAGM 683
           LF    +D      NP S V+P HL+YF F GRV+ LA+ H   +   F   F+  +   
Sbjct: 525 LFEYSAHDNYTLQINPHSGVNPEHLNYFKFIGRVVGLAIFHRRFLDSFFIGAFYKMMLRK 584

Query: 684 CISLEDIRDADPYLYGSCKQILDMDAELVDSDVLGLTFASDFEELGTREVVDLCPGGKDM 743
            +SL+D+   D  L+ +    LD D E     ++ LTFA D E+ G R  +DL PGG+D+
Sbjct: 585 KVSLQDMEGVDEDLHRNLTWTLDNDIE----GIIELTFAVDDEKFGERRTIDLKPGGRDI 644

Query: 744 VVNSKNRKEYVQLLVENRFMKSVSKQISYFANGFTDVLADKRSHKCFFQSLELEDLDWML 803
            V ++N+ EYV+L+ E + +K V +Q + F +GF +++     +   F   ELE    +L
Sbjct: 645 PVTNENKGEYVELVTEWKIVKRVEEQFNAFMSGFNELIPADLVN--VFDERELE----LL 704

Query: 804 YGSESQISVEDWKAHTEYSGYKETDPQISWFWKIVSAMTPEQRVNLLFFWTSVKYLPIQG 863
            G  + I V+DWK HT+Y GY+E+D  I  FWKIV     EQ+  LL F T    +P+ G
Sbjct: 705 IGGIADIDVDDWKKHTDYRGYQESDEVIQNFWKIVRTWDAEQKSRLLQFTTGTSRIPVNG 764

Query: 864 FNGLAS-----KLYIYKSSSPYDHLPSSHTCFFRLCFPPYPSELIMKRRLEIITQEHIG 916
           F  L       +  I KS  P   LP SHTCF RL  PPY +  +++ +L I  +E +G
Sbjct: 765 FKDLQGSDGPRRFTIEKSGDP-GALPKSHTCFNRLDLPPYKTNDVLEHKLSIAVEETLG 812

BLAST of Cp4.1LG10g10030.1 vs. ExPASy Swiss-Prot
Match: B0XQ72 (Probable E3 ubiquitin-protein ligase hulA OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=hulA PE=3 SV=2)

HSP 1 Score: 214.9 bits (546), Expect = 4.6e-54
Identity = 130/358 (36.31%), Postives = 190/358 (53.07%), Query Frame = 0

Query: 564 IDRSQLLEESFEYITNASVEALRHGLFVLFKNEEATGPGVL-REWFLLVCKAIFNPQNAL 623
           + R+ + E+S+  I   S   L+  L + F  E+    G L RE+F L+   +FNP   L
Sbjct: 463 VRRNNIFEDSYAEIMRQSASDLKKRLMIKFDGEDGLDYGGLSREFFFLLSHEMFNPFYCL 522

Query: 624 FVACPNDRRRFFPNPVSKVDPMHLSYFSFSGRVIALALMHNVQVGVVFDRVFFLQLAGMC 683
           F    +D      NP S V+P HL+YF F GRV+ LA+ H   +   F   F+  +    
Sbjct: 523 FEYSAHDNYTLQINPHSGVNPEHLNYFKFIGRVVGLAIFHRRFLDSFFIGAFYKMMLRKK 582

Query: 684 ISLEDIRDADPYLYGSCKQILDMDAELVDSDVLGLTFASDFEELGTREVVDLCPGGKDMV 743
           +SL+D+   D  L+ +    LD D E     VL LTF+ D E+ G R  +DL PGG+D+ 
Sbjct: 583 VSLQDMEGVDEDLHRNLTWTLDNDIE----GVLELTFSVDDEKFGERRTIDLKPGGRDIP 642

Query: 744 VNSKNRKEYVQLLVENRFMKSVSKQISYFANGFTDVLADKRSHKCFFQSLELEDLDWMLY 803
           V ++N+ EYV+L+ E + +K V +Q + F +GF +++     +   F   ELE    +L 
Sbjct: 643 VTNENKAEYVELVTEWKIVKRVEEQFNAFMSGFNELIPADLVN--VFDERELE----LLI 702

Query: 804 GSESQISVEDWKAHTEYSGYKETDPQISWFWKIVSAMTPEQRVNLLFFWTSVKYLPIQGF 863
           G  + I V+DWK HT+Y GY+E+D  I  FWKIV +   EQ+  LL F T    +P+ GF
Sbjct: 703 GGIADIDVDDWKKHTDYRGYQESDEVIQNFWKIVRSWDAEQKSRLLQFTTGTSRIPVNGF 762

Query: 864 NGLAS-----KLYIYKSSSPYDHLPSSHTCFFRLCFPPYPSELIMKRRLEIITQEHIG 916
             L       +  I KS  P   LP SHTCF RL  PPY S   ++ ++ I  +E +G
Sbjct: 763 KDLQGSDGPRRFTIEKSGDP-AALPKSHTCFNRLDLPPYKSYETLEHKMSIAVEETLG 809

BLAST of Cp4.1LG10g10030.1 vs. ExPASy Swiss-Prot
Match: Q4WTF3 (Probable E3 ubiquitin-protein ligase hulA OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=hulA PE=3 SV=2)

HSP 1 Score: 214.9 bits (546), Expect = 4.6e-54
Identity = 130/358 (36.31%), Postives = 190/358 (53.07%), Query Frame = 0

Query: 564 IDRSQLLEESFEYITNASVEALRHGLFVLFKNEEATGPGVL-REWFLLVCKAIFNPQNAL 623
           + R+ + E+S+  I   S   L+  L + F  E+    G L RE+F L+   +FNP   L
Sbjct: 463 VRRNNIFEDSYAEIMRQSASDLKKRLMIKFDGEDGLDYGGLSREFFFLLSHEMFNPFYCL 522

Query: 624 FVACPNDRRRFFPNPVSKVDPMHLSYFSFSGRVIALALMHNVQVGVVFDRVFFLQLAGMC 683
           F    +D      NP S V+P HL+YF F GRV+ LA+ H   +   F   F+  +    
Sbjct: 523 FEYSAHDNYTLQINPHSGVNPEHLNYFKFIGRVVGLAIFHRRFLDSFFIGAFYKMMLRKK 582

Query: 684 ISLEDIRDADPYLYGSCKQILDMDAELVDSDVLGLTFASDFEELGTREVVDLCPGGKDMV 743
           +SL+D+   D  L+ +    LD D E     VL LTF+ D E+ G R  +DL PGG+D+ 
Sbjct: 583 VSLQDMEGVDEDLHRNLTWTLDNDIE----GVLELTFSVDDEKFGERRTIDLKPGGRDIP 642

Query: 744 VNSKNRKEYVQLLVENRFMKSVSKQISYFANGFTDVLADKRSHKCFFQSLELEDLDWMLY 803
           V ++N+ EYV+L+ E + +K V +Q + F +GF +++     +   F   ELE    +L 
Sbjct: 643 VTNENKAEYVELVTEWKIVKRVEEQFNAFMSGFNELIPADLVN--VFDERELE----LLI 702

Query: 804 GSESQISVEDWKAHTEYSGYKETDPQISWFWKIVSAMTPEQRVNLLFFWTSVKYLPIQGF 863
           G  + I V+DWK HT+Y GY+E+D  I  FWKIV +   EQ+  LL F T    +P+ GF
Sbjct: 703 GGIADIDVDDWKKHTDYRGYQESDEVIQNFWKIVRSWDAEQKSRLLQFTTGTSRIPVNGF 762

Query: 864 NGLAS-----KLYIYKSSSPYDHLPSSHTCFFRLCFPPYPSELIMKRRLEIITQEHIG 916
             L       +  I KS  P   LP SHTCF RL  PPY S   ++ ++ I  +E +G
Sbjct: 763 KDLQGSDGPRRFTIEKSGDP-AALPKSHTCFNRLDLPPYKSYETLEHKMSIAVEETLG 809

BLAST of Cp4.1LG10g10030.1 vs. NCBI nr
Match: XP_023544037.1 (E3 ubiquitin-protein ligase UPL5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1781 bits (4613), Expect = 0.0
Identity = 896/918 (97.60%), Postives = 896/918 (97.60%), Query Frame = 0

Query: 1   GTADAEHCLLNRHNSQGHRHPCVSSKRKLDDYAASLDDEDEDAPLFDLISIRMKKDETSA 60
           GTADAEHCLLNRHNSQGHRHPCVSSKRKLDDYAASLDDEDEDAPLFDLISIRMKKDETSA
Sbjct: 18  GTADAEHCLLNRHNSQGHRHPCVSSKRKLDDYAASLDDEDEDAPLFDLISIRMKKDETSA 77

Query: 61  IDSSFDGRLGEGTSSDFEYRGFDDSSTSMAAETSLKPILSPHELQFFVRTMSVGNTMVML 120
           IDSSFDGRLGEGTSSDFEYRGFDDSSTSMAAETSLKPILSPHELQFFVRTMSVGNTMVML
Sbjct: 78  IDSSFDGRLGEGTSSDFEYRGFDDSSTSMAAETSLKPILSPHELQFFVRTMSVGNTMVML 137

Query: 121 ANTNDTVMSLHERIQSITRIPVFEQRLIYRGRQLQHEQSLRECSIQNNAELQLVGRMRST 180
           ANTNDTVMSLHERIQSITRIPVFEQRLIYRGRQLQHEQSLRECSIQNNAELQLVGRMRST
Sbjct: 138 ANTNDTVMSLHERIQSITRIPVFEQRLIYRGRQLQHEQSLRECSIQNNAELQLVGRMRST 197

Query: 181 EHPKAWQIVDDMVSLVLRLYRGEVVFSASEILTTLMTDFLSLATQTDLDPAMKQLQVFLS 240
           EHPKAWQIVDDMVSLVLRLYRGEVVFSASEILTTLMTDFLSLATQTDLDPAMKQLQVFLS
Sbjct: 198 EHPKAWQIVDDMVSLVLRLYRGEVVFSASEILTTLMTDFLSLATQTDLDPAMKQLQVFLS 257

Query: 241 LSAPAALVMLYLSPIKGNTDCADNLIKHFMDLLCHSVPKSLKKCCAIIVLEFCNLLRRDT 300
           LSAPAALVMLYLSPIKGNTDCADNLIKHFMDLLCHSVPKSLKKCCAIIVLEFCNLLRRDT
Sbjct: 258 LSAPAALVMLYLSPIKGNTDCADNLIKHFMDLLCHSVPKSLKKCCAIIVLEFCNLLRRDT 317

Query: 301 PEDSLYVLCRSTLGSLLETDGNSRGMRCLESVWGPIKTPELFPFVNELANKLSIDLSSSI 360
           PEDSLYVLCRSTLGSLLETDGNSRGMRCLESVWGPIKTPELFPFVNELANKLSIDLSSSI
Sbjct: 318 PEDSLYVLCRSTLGSLLETDGNSRGMRCLESVWGPIKTPELFPFVNELANKLSIDLSSSI 377

Query: 361 RSPTSAGPSXANKLSIELSSSIRSPTSAGPSVTDISDFTAFLLPLRNAIPEKLSFHGSKN 420
                                 RSPTSAGPSVTDISDFTAFLLPLRNAIPEKLSFHGSKN
Sbjct: 378 ----------------------RSPTSAGPSVTDISDFTAFLLPLRNAIPEKLSFHGSKN 437

Query: 421 VPLDRGGFRDSSYGEEGEFLHNIYLSLLKQMDICLQGMEAFLTDKGKGNCVIPYIGWSQY 480
           VPLDRGGFRDSSYGEEGEFLHNIYLSLLKQMDICLQGMEAFLTDKGKGNCVIPYIGWSQY
Sbjct: 438 VPLDRGGFRDSSYGEEGEFLHNIYLSLLKQMDICLQGMEAFLTDKGKGNCVIPYIGWSQY 497

Query: 481 LPILKELNDISLLFQGLKEEFWAIMRPRKSSICELIIRFAKRADDYSWILCHKDVINSES 540
           LPILKELNDISLLFQGLKEEFWAIMRPRKSSICELIIRFAKRADDYSWILCHKDVINSES
Sbjct: 498 LPILKELNDISLLFQGLKEEFWAIMRPRKSSICELIIRFAKRADDYSWILCHKDVINSES 557

Query: 541 RRHLSMLMFPEPTEDYEELQEMLIDRSQLLEESFEYITNASVEALRHGLFVLFKNEEATG 600
           RRHLSMLMFPEPTEDYEELQEMLIDRSQLLEESFEYITNASVEALRHGLFVLFKNEEATG
Sbjct: 558 RRHLSMLMFPEPTEDYEELQEMLIDRSQLLEESFEYITNASVEALRHGLFVLFKNEEATG 617

Query: 601 PGVLREWFLLVCKAIFNPQNALFVACPNDRRRFFPNPVSKVDPMHLSYFSFSGRVIALAL 660
           PGVLREWFLLVCKAIFNPQNALFVACPNDRRRFFPNPVSKVDPMHLSYFSFSGRVIALAL
Sbjct: 618 PGVLREWFLLVCKAIFNPQNALFVACPNDRRRFFPNPVSKVDPMHLSYFSFSGRVIALAL 677

Query: 661 MHNVQVGVVFDRVFFLQLAGMCISLEDIRDADPYLYGSCKQILDMDAELVDSDVLGLTFA 720
           MHNVQVGVVFDRVFFLQLAGMCISLEDIRDADPYLYGSCKQILDMDAELVDSDVLGLTFA
Sbjct: 678 MHNVQVGVVFDRVFFLQLAGMCISLEDIRDADPYLYGSCKQILDMDAELVDSDVLGLTFA 737

Query: 721 SDFEELGTREVVDLCPGGKDMVVNSKNRKEYVQLLVENRFMKSVSKQISYFANGFTDVLA 780
           SDFEELGTREVVDLCPGGKDMVVNSKNRKEYVQLLVENRFMKSVSKQISYFANGFTDVLA
Sbjct: 738 SDFEELGTREVVDLCPGGKDMVVNSKNRKEYVQLLVENRFMKSVSKQISYFANGFTDVLA 797

Query: 781 DKRSHKCFFQSLELEDLDWMLYGSESQISVEDWKAHTEYSGYKETDPQISWFWKIVSAMT 840
           DKRSHKCFFQSLELEDLDWMLYGSESQISVEDWKAHTEYSGYKETDPQISWFWKIVSAMT
Sbjct: 798 DKRSHKCFFQSLELEDLDWMLYGSESQISVEDWKAHTEYSGYKETDPQISWFWKIVSAMT 857

Query: 841 PEQRVNLLFFWTSVKYLPIQGFNGLASKLYIYKSSSPYDHLPSSHTCFFRLCFPPYPSEL 900
           PEQRVNLLFFWTSVKYLPIQGFNGLASKLYIYKSSSPYDHLPSSHTCFFRLCFPPYPSEL
Sbjct: 858 PEQRVNLLFFWTSVKYLPIQGFNGLASKLYIYKSSSPYDHLPSSHTCFFRLCFPPYPSEL 913

Query: 901 IMKRRLEIITQEHIGCSF 918
           IMKRRLEIITQEHIGCSF
Sbjct: 918 IMKRRLEIITQEHIGCSF 913

BLAST of Cp4.1LG10g10030.1 vs. NCBI nr
Match: XP_023522098.1 (E3 ubiquitin-protein ligase UPL5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1776 bits (4600), Expect = 0.0
Identity = 894/918 (97.39%), Postives = 895/918 (97.49%), Query Frame = 0

Query: 1   GTADAEHCLLNRHNSQGHRHPCVSSKRKLDDYAASLDDEDEDAPLFDLISIRMKKDETSA 60
           GTADAEHCLLNRHNSQGHRHPCVSSKRKLDDYAASLDDEDEDAPLFDLISIRMKKDETSA
Sbjct: 18  GTADAEHCLLNRHNSQGHRHPCVSSKRKLDDYAASLDDEDEDAPLFDLISIRMKKDETSA 77

Query: 61  IDSSFDGRLGEGTSSDFEYRGFDDSSTSMAAETSLKPILSPHELQFFVRTMSVGNTMVML 120
           IDSSFDGRLGEGTSSDFEYRGFDDSSTSMAAETSLKPILSPHELQFFVRTMSVGNTMVML
Sbjct: 78  IDSSFDGRLGEGTSSDFEYRGFDDSSTSMAAETSLKPILSPHELQFFVRTMSVGNTMVML 137

Query: 121 ANTNDTVMSLHERIQSITRIPVFEQRLIYRGRQLQHEQSLRECSIQNNAELQLVGRMRST 180
           ANTNDTVMSLHERIQSITRIPVFEQRLIYRGRQLQHEQSLRECSIQNNAELQLVGRMRST
Sbjct: 138 ANTNDTVMSLHERIQSITRIPVFEQRLIYRGRQLQHEQSLRECSIQNNAELQLVGRMRST 197

Query: 181 EHPKAWQIVDDMVSLVLRLYRGEVVFSASEILTTLMTDFLSLATQTDLDPAMKQLQVFLS 240
           EHPKAWQIVDDMVSLVLRLYRGEVVFSASEILTTLMTDFLSLATQTDLDPAMKQLQVFLS
Sbjct: 198 EHPKAWQIVDDMVSLVLRLYRGEVVFSASEILTTLMTDFLSLATQTDLDPAMKQLQVFLS 257

Query: 241 LSAPAALVMLYLSPIKGNTDCADNLIKHFMDLLCHSVPKSLKKCCAIIVLEFCNLLRRDT 300
           LSAPAALVMLYLSPIKGNTDCADNLIKHFMDLLCHSVPKSLKKCCAIIVLEFCNLLRRDT
Sbjct: 258 LSAPAALVMLYLSPIKGNTDCADNLIKHFMDLLCHSVPKSLKKCCAIIVLEFCNLLRRDT 317

Query: 301 PEDSLYVLCRSTLGSLLETDGNSRGMRCLESVWGPIKTPELFPFVNELANKLSIDLSSSI 360
           PEDSLYVLCRSTLGSLLETDGNSRGMRCLESVWGPIKTPELFPFVNELANKLSI      
Sbjct: 318 PEDSLYVLCRSTLGSLLETDGNSRGMRCLESVWGPIKTPELFPFVNELANKLSI------ 377

Query: 361 RSPTSAGPSXANKLSIELSSSIRSPTSAGPSVTDISDFTAFLLPLRNAIPEKLSFHGSKN 420
                           ELSSSIRSPTSAGPSVTDISDFTAFLLPLRNAIPEKLSFHGSKN
Sbjct: 378 ----------------ELSSSIRSPTSAGPSVTDISDFTAFLLPLRNAIPEKLSFHGSKN 437

Query: 421 VPLDRGGFRDSSYGEEGEFLHNIYLSLLKQMDICLQGMEAFLTDKGKGNCVIPYIGWSQY 480
           VPLDRGGFRDSSYGEEGEFLHNIYLSLLKQMDICLQGMEAFLTDKGKGNCVIPYIGWSQY
Sbjct: 438 VPLDRGGFRDSSYGEEGEFLHNIYLSLLKQMDICLQGMEAFLTDKGKGNCVIPYIGWSQY 497

Query: 481 LPILKELNDISLLFQGLKEEFWAIMRPRKSSICELIIRFAKRADDYSWILCHKDVINSES 540
           LPILKELNDISLLFQGLKEEFWAIMRPRKSSICELIIRFAKRADDYSWILCHKDVINSES
Sbjct: 498 LPILKELNDISLLFQGLKEEFWAIMRPRKSSICELIIRFAKRADDYSWILCHKDVINSES 557

Query: 541 RRHLSMLMFPEPTEDYEELQEMLIDRSQLLEESFEYITNASVEALRHGLFVLFKNEEATG 600
           RRHLSMLMFPEPTEDYEELQEMLIDRSQLLEESFEYITNASVEALRHGLFVLFKNEEATG
Sbjct: 558 RRHLSMLMFPEPTEDYEELQEMLIDRSQLLEESFEYITNASVEALRHGLFVLFKNEEATG 617

Query: 601 PGVLREWFLLVCKAIFNPQNALFVACPNDRRRFFPNPVSKVDPMHLSYFSFSGRVIALAL 660
           PGVLREWFLLVCKAIFNPQNALFVACPNDRRRFFPNPVSKVDPMHLSYFSFSGRVIALAL
Sbjct: 618 PGVLREWFLLVCKAIFNPQNALFVACPNDRRRFFPNPVSKVDPMHLSYFSFSGRVIALAL 677

Query: 661 MHNVQVGVVFDRVFFLQLAGMCISLEDIRDADPYLYGSCKQILDMDAELVDSDVLGLTFA 720
           MHNVQVGVVFDRVFFLQLAGMCISLEDIRDADPYLYGSCKQILDMDAELVDSDVLGLTFA
Sbjct: 678 MHNVQVGVVFDRVFFLQLAGMCISLEDIRDADPYLYGSCKQILDMDAELVDSDVLGLTFA 737

Query: 721 SDFEELGTREVVDLCPGGKDMVVNSKNRKEYVQLLVENRFMKSVSKQISYFANGFTDVLA 780
           SDFEELGTREVVDLCPGGKDMVVNSKNRKEYVQLLVENRFMKSVSKQISYFANGFTDVLA
Sbjct: 738 SDFEELGTREVVDLCPGGKDMVVNSKNRKEYVQLLVENRFMKSVSKQISYFANGFTDVLA 797

Query: 781 DKRSHKCFFQSLELEDLDWMLYGSESQISVEDWKAHTEYSGYKETDPQISWFWKIVSAMT 840
           DKRSHKCFFQSLELEDLDWMLYGSESQISVEDWKAHTEYSGYKETDPQISWFWKIVSAMT
Sbjct: 798 DKRSHKCFFQSLELEDLDWMLYGSESQISVEDWKAHTEYSGYKETDPQISWFWKIVSAMT 857

Query: 841 PEQRVNLLFFWTSVKYLPIQGFNGLASKLYIYKSSSPYDHLPSSHTCFFRLCFPPYPSEL 900
           PEQRVNLLFFWTSVKYLPIQGFNGLASKLYIYKSSSPYDHLPSSHTCFFRLCFPPYPS+ 
Sbjct: 858 PEQRVNLLFFWTSVKYLPIQGFNGLASKLYIYKSSSPYDHLPSSHTCFFRLCFPPYPSKS 913

Query: 901 IMKRRLEIITQEHIGCSF 918
           IMKRRLEIITQEHIGCSF
Sbjct: 918 IMKRRLEIITQEHIGCSF 913

BLAST of Cp4.1LG10g10030.1 vs. NCBI nr
Match: XP_022950558.1 (E3 ubiquitin-protein ligase UPL5-like [Cucurbita moschata])

HSP 1 Score: 1772 bits (4590), Expect = 0.0
Identity = 892/918 (97.17%), Postives = 893/918 (97.28%), Query Frame = 0

Query: 1   GTADAEHCLLNRHNSQGHRHPCVSSKRKLDDYAASLDDEDEDAPLFDLISIRMKKDETSA 60
           GTADAEHCLLNRHNSQGHRH CVSSKRKLDDYAASLDDEDEDAPLFDLISIRMKKDETSA
Sbjct: 18  GTADAEHCLLNRHNSQGHRHHCVSSKRKLDDYAASLDDEDEDAPLFDLISIRMKKDETSA 77

Query: 61  IDSSFDGRLGEGTSSDFEYRGFDDSSTSMAAETSLKPILSPHELQFFVRTMSVGNTMVML 120
           IDSSFDGRLGEGTSSDFEYRGFDDSSTSMAAETS KPILSPHELQFFVRTMSVGNTMVML
Sbjct: 78  IDSSFDGRLGEGTSSDFEYRGFDDSSTSMAAETSPKPILSPHELQFFVRTMSVGNTMVML 137

Query: 121 ANTNDTVMSLHERIQSITRIPVFEQRLIYRGRQLQHEQSLRECSIQNNAELQLVGRMRST 180
           ANTNDTVMSLHERIQSITRIPVFEQRLIYRGRQLQHEQSLRECSIQNNAELQLVGRMRST
Sbjct: 138 ANTNDTVMSLHERIQSITRIPVFEQRLIYRGRQLQHEQSLRECSIQNNAELQLVGRMRST 197

Query: 181 EHPKAWQIVDDMVSLVLRLYRGEVVFSASEILTTLMTDFLSLATQTDLDPAMKQLQVFLS 240
           EHPKAWQIVDDMVSLVLRLYRGEVVFSASEILTTLMTDFLSLATQTDLDPAMKQLQVFLS
Sbjct: 198 EHPKAWQIVDDMVSLVLRLYRGEVVFSASEILTTLMTDFLSLATQTDLDPAMKQLQVFLS 257

Query: 241 LSAPAALVMLYLSPIKGNTDCADNLIKHFMDLLCHSVPKSLKKCCAIIVLEFCNLLRRDT 300
           LSAPAALVMLYLSPIKGNTDCADNLIKHFMDLLCHSVPKSLKKCCAIIVLEFCNLLRRDT
Sbjct: 258 LSAPAALVMLYLSPIKGNTDCADNLIKHFMDLLCHSVPKSLKKCCAIIVLEFCNLLRRDT 317

Query: 301 PEDSLYVLCRSTLGSLLETDGNSRGMRCLESVWGPIKTPELFPFVNELANKLSIDLSSSI 360
           PEDSLYVLCRSTLGSLLETDGNSRGMRCLESVWGPIKTPELFPFVNELANKLSIDLSSSI
Sbjct: 318 PEDSLYVLCRSTLGSLLETDGNSRGMRCLESVWGPIKTPELFPFVNELANKLSIDLSSSI 377

Query: 361 RSPTSAGPSXANKLSIELSSSIRSPTSAGPSVTDISDFTAFLLPLRNAIPEKLSFHGSKN 420
                                 RSPTSAGPSVTDISDFTAFLLPLRNAIPEKLSFHGSKN
Sbjct: 378 ----------------------RSPTSAGPSVTDISDFTAFLLPLRNAIPEKLSFHGSKN 437

Query: 421 VPLDRGGFRDSSYGEEGEFLHNIYLSLLKQMDICLQGMEAFLTDKGKGNCVIPYIGWSQY 480
           VPLDRGGFRDSSYGEEGEFLHNIYLSLLKQMDICLQGMEAFLTDKGKGNCVIPYIGWSQY
Sbjct: 438 VPLDRGGFRDSSYGEEGEFLHNIYLSLLKQMDICLQGMEAFLTDKGKGNCVIPYIGWSQY 497

Query: 481 LPILKELNDISLLFQGLKEEFWAIMRPRKSSICELIIRFAKRADDYSWILCHKDVINSES 540
           LPILKELNDISLLFQGLKEEFWAIMRPRKSSICELIIRFAKRADDYSWILCHKDVINSES
Sbjct: 498 LPILKELNDISLLFQGLKEEFWAIMRPRKSSICELIIRFAKRADDYSWILCHKDVINSES 557

Query: 541 RRHLSMLMFPEPTEDYEELQEMLIDRSQLLEESFEYITNASVEALRHGLFVLFKNEEATG 600
           RRHLSMLMFPEPTEDYEELQEMLIDRSQLLEESFEYITNASVEALRHGLFVLFKNEEATG
Sbjct: 558 RRHLSMLMFPEPTEDYEELQEMLIDRSQLLEESFEYITNASVEALRHGLFVLFKNEEATG 617

Query: 601 PGVLREWFLLVCKAIFNPQNALFVACPNDRRRFFPNPVSKVDPMHLSYFSFSGRVIALAL 660
           PGVLREWFLLVCKAIFNPQNALFVACPNDRRRFFPNPVSKVDPMHLSYFSFSGRVIALAL
Sbjct: 618 PGVLREWFLLVCKAIFNPQNALFVACPNDRRRFFPNPVSKVDPMHLSYFSFSGRVIALAL 677

Query: 661 MHNVQVGVVFDRVFFLQLAGMCISLEDIRDADPYLYGSCKQILDMDAELVDSDVLGLTFA 720
           MHNVQVGVVFDRVFFLQLAGMCISLEDIRDADPYLYGSCKQILDMDAELVDSDVLGLTFA
Sbjct: 678 MHNVQVGVVFDRVFFLQLAGMCISLEDIRDADPYLYGSCKQILDMDAELVDSDVLGLTFA 737

Query: 721 SDFEELGTREVVDLCPGGKDMVVNSKNRKEYVQLLVENRFMKSVSKQISYFANGFTDVLA 780
           SDFEELGTREVVDLCPGGKDMVVNSKNRKEYVQLL+ENRFMKSVSKQISYFANGFTDVLA
Sbjct: 738 SDFEELGTREVVDLCPGGKDMVVNSKNRKEYVQLLIENRFMKSVSKQISYFANGFTDVLA 797

Query: 781 DKRSHKCFFQSLELEDLDWMLYGSESQISVEDWKAHTEYSGYKETDPQISWFWKIVSAMT 840
           DKRSHKCFFQSLELEDLDWMLYGSESQISVEDWKAHTEYSGYKETDPQISWFWKIVSAMT
Sbjct: 798 DKRSHKCFFQSLELEDLDWMLYGSESQISVEDWKAHTEYSGYKETDPQISWFWKIVSAMT 857

Query: 841 PEQRVNLLFFWTSVKYLPIQGFNGLASKLYIYKSSSPYDHLPSSHTCFFRLCFPPYPSEL 900
           PEQRVNLLFFWTSVKYLPIQGFNGLASKLYIYKSSSPYDHLPSSHTCFFRLCFPPYPSE 
Sbjct: 858 PEQRVNLLFFWTSVKYLPIQGFNGLASKLYIYKSSSPYDHLPSSHTCFFRLCFPPYPSES 913

Query: 901 IMKRRLEIITQEHIGCSF 918
           IMKRRLEIITQEHIGCSF
Sbjct: 918 IMKRRLEIITQEHIGCSF 913

BLAST of Cp4.1LG10g10030.1 vs. NCBI nr
Match: KAG7033999.1 (E3 ubiquitin-protein ligase UPL5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1766 bits (4573), Expect = 0.0
Identity = 889/918 (96.84%), Postives = 891/918 (97.06%), Query Frame = 0

Query: 1   GTADAEHCLLNRHNSQGHRHPCVSSKRKLDDYAASLDDEDEDAPLFDLISIRMKKDETSA 60
           GTADAEHCLLNRHNSQGHRHPCVSSKRKLDDYAASLDDEDEDAPLFDLISIRMKKDETSA
Sbjct: 18  GTADAEHCLLNRHNSQGHRHPCVSSKRKLDDYAASLDDEDEDAPLFDLISIRMKKDETSA 77

Query: 61  IDSSFDGRLGEGTSSDFEYRGFDDSSTSMAAETSLKPILSPHELQFFVRTMSVGNTMVML 120
           IDSSFDGRLGEGTSSDFEYRGFDDSSTSMAAETS KPILSPHELQFFVRTMSVGNTMVML
Sbjct: 78  IDSSFDGRLGEGTSSDFEYRGFDDSSTSMAAETSPKPILSPHELQFFVRTMSVGNTMVML 137

Query: 121 ANTNDTVMSLHERIQSITRIPVFEQRLIYRGRQLQHEQSLRECSIQNNAELQLVGRMRST 180
           ANTNDTVMSLHERIQSITRIPVFEQRLIYRGRQLQHEQSLRECSIQNNAELQLVGRMRST
Sbjct: 138 ANTNDTVMSLHERIQSITRIPVFEQRLIYRGRQLQHEQSLRECSIQNNAELQLVGRMRST 197

Query: 181 EHPKAWQIVDDMVSLVLRLYRGEVVFSASEILTTLMTDFLSLATQTDLDPAMKQLQVFLS 240
           EHPKAWQIVDDMVSLVLRLYRGEVVFSASEILTTLMTDFLSLATQTDLDPAMKQLQVFLS
Sbjct: 198 EHPKAWQIVDDMVSLVLRLYRGEVVFSASEILTTLMTDFLSLATQTDLDPAMKQLQVFLS 257

Query: 241 LSAPAALVMLYLSPIKGNTDCADNLIKHFMDLLCHSVPKSLKKCCAIIVLEFCNLLRRDT 300
           LSAPAALVMLYLSPIKGNTDCADNLIKHFMDLLCHSVPKSLKKCCAIIVLEFCNLLRRDT
Sbjct: 258 LSAPAALVMLYLSPIKGNTDCADNLIKHFMDLLCHSVPKSLKKCCAIIVLEFCNLLRRDT 317

Query: 301 PEDSLYVLCRSTLGSLLETDGNSRGMRCLESVWGPIKTPELFPFVNELANKLSIDLSSSI 360
           PEDSLYVLCRSTLGSLLETDGNSRGMRCLESVWGPIKTPELFPFVNELANKLSIDLSSSI
Sbjct: 318 PEDSLYVLCRSTLGSLLETDGNSRGMRCLESVWGPIKTPELFPFVNELANKLSIDLSSSI 377

Query: 361 RSPTSAGPSXANKLSIELSSSIRSPTSAGPSVTDISDFTAFLLPLRNAIPEKLSFHGSKN 420
                                 RSPTSAGPSVTDISDFTAFLLPLRNAIPEKLSFHGSKN
Sbjct: 378 ----------------------RSPTSAGPSVTDISDFTAFLLPLRNAIPEKLSFHGSKN 437

Query: 421 VPLDRGGFRDSSYGEEGEFLHNIYLSLLKQMDICLQGMEAFLTDKGKGNCVIPYIGWSQY 480
           VPLDRG +RDSSYGEEGEFL NIYLSLLKQMDICLQGMEAFLTDKGKGNCVIPYIGWSQY
Sbjct: 438 VPLDRGDYRDSSYGEEGEFLLNIYLSLLKQMDICLQGMEAFLTDKGKGNCVIPYIGWSQY 497

Query: 481 LPILKELNDISLLFQGLKEEFWAIMRPRKSSICELIIRFAKRADDYSWILCHKDVINSES 540
           LPILKELNDISLLFQGLKEEFWAIMRPRKSSICELIIRFAKRADDYSWILCHKDVINSES
Sbjct: 498 LPILKELNDISLLFQGLKEEFWAIMRPRKSSICELIIRFAKRADDYSWILCHKDVINSES 557

Query: 541 RRHLSMLMFPEPTEDYEELQEMLIDRSQLLEESFEYITNASVEALRHGLFVLFKNEEATG 600
           RRHLSMLMFPEPTEDYEELQEMLIDRSQLLEESFEYITNASVEALRHGLFVLFKNEEATG
Sbjct: 558 RRHLSMLMFPEPTEDYEELQEMLIDRSQLLEESFEYITNASVEALRHGLFVLFKNEEATG 617

Query: 601 PGVLREWFLLVCKAIFNPQNALFVACPNDRRRFFPNPVSKVDPMHLSYFSFSGRVIALAL 660
           PGVLREWFLLVCKAIFNPQNALFVACPNDRRRFFPNPVSKVDPMHLSYFSFSGRVIALAL
Sbjct: 618 PGVLREWFLLVCKAIFNPQNALFVACPNDRRRFFPNPVSKVDPMHLSYFSFSGRVIALAL 677

Query: 661 MHNVQVGVVFDRVFFLQLAGMCISLEDIRDADPYLYGSCKQILDMDAELVDSDVLGLTFA 720
           MHNVQVGVVFDRVFFLQLAGMCISLEDIRDADPYLYGSCKQILDMDAELVDSDVLGLTFA
Sbjct: 678 MHNVQVGVVFDRVFFLQLAGMCISLEDIRDADPYLYGSCKQILDMDAELVDSDVLGLTFA 737

Query: 721 SDFEELGTREVVDLCPGGKDMVVNSKNRKEYVQLLVENRFMKSVSKQISYFANGFTDVLA 780
           SDFEELGTRE VDLCPGGKDMVVNSKNRKEYVQLL+ENRFMKSVSKQISYFANGFTDVLA
Sbjct: 738 SDFEELGTREFVDLCPGGKDMVVNSKNRKEYVQLLIENRFMKSVSKQISYFANGFTDVLA 797

Query: 781 DKRSHKCFFQSLELEDLDWMLYGSESQISVEDWKAHTEYSGYKETDPQISWFWKIVSAMT 840
           DKRSHKCFFQSLELEDLDWMLYGSESQISVEDWKAHTEYSGYKETDPQISWFWKIVSAMT
Sbjct: 798 DKRSHKCFFQSLELEDLDWMLYGSESQISVEDWKAHTEYSGYKETDPQISWFWKIVSAMT 857

Query: 841 PEQRVNLLFFWTSVKYLPIQGFNGLASKLYIYKSSSPYDHLPSSHTCFFRLCFPPYPSEL 900
           PEQRVNLLFFWTSVKYLPIQGFNGLASKLYIYKSSSPYDHLPSSHTCFFRLCFPPYPSE 
Sbjct: 858 PEQRVNLLFFWTSVKYLPIQGFNGLASKLYIYKSSSPYDHLPSSHTCFFRLCFPPYPSES 913

Query: 901 IMKRRLEIITQEHIGCSF 918
           IMKRRLEIITQEHIGCSF
Sbjct: 918 IMKRRLEIITQEHIGCSF 913

BLAST of Cp4.1LG10g10030.1 vs. NCBI nr
Match: KAG6603818.1 (E3 ubiquitin-protein ligase UPL5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1764 bits (4569), Expect = 0.0
Identity = 888/918 (96.73%), Postives = 890/918 (96.95%), Query Frame = 0

Query: 1    GTADAEHCLLNRHNSQGHRHPCVSSKRKLDDYAASLDDEDEDAPLFDLISIRMKKDETSA 60
            GTADAEHCLLNRHNSQGHRHPCVSSKRKLDDYAASLDDEDEDAPLFDLISIRMKKDETSA
Sbjct: 109  GTADAEHCLLNRHNSQGHRHPCVSSKRKLDDYAASLDDEDEDAPLFDLISIRMKKDETSA 168

Query: 61   IDSSFDGRLGEGTSSDFEYRGFDDSSTSMAAETSLKPILSPHELQFFVRTMSVGNTMVML 120
            IDSSFDGRLGEGTSSDFEYRGFDDSSTSMAAETS KPILSPHELQFFVRTMSVGNTMVML
Sbjct: 169  IDSSFDGRLGEGTSSDFEYRGFDDSSTSMAAETSPKPILSPHELQFFVRTMSVGNTMVML 228

Query: 121  ANTNDTVMSLHERIQSITRIPVFEQRLIYRGRQLQHEQSLRECSIQNNAELQLVGRMRST 180
            ANTNDTVMSLHERIQSITRIPVFEQRLIYRGRQLQHEQSLRECSIQNNAELQLVGRMRST
Sbjct: 229  ANTNDTVMSLHERIQSITRIPVFEQRLIYRGRQLQHEQSLRECSIQNNAELQLVGRMRST 288

Query: 181  EHPKAWQIVDDMVSLVLRLYRGEVVFSASEILTTLMTDFLSLATQTDLDPAMKQLQVFLS 240
            EHPKAWQIVDDMVSLVLRLYRGEVVFSASEILTTLMTDFLSLATQTDLDPAMKQLQVFLS
Sbjct: 289  EHPKAWQIVDDMVSLVLRLYRGEVVFSASEILTTLMTDFLSLATQTDLDPAMKQLQVFLS 348

Query: 241  LSAPAALVMLYLSPIKGNTDCADNLIKHFMDLLCHSVPKSLKKCCAIIVLEFCNLLRRDT 300
            LSAPAALVMLYLSPIKGNTDCADNLIKHFMDLLCHSVPKSLKKCCAIIVLEFCNLLRRDT
Sbjct: 349  LSAPAALVMLYLSPIKGNTDCADNLIKHFMDLLCHSVPKSLKKCCAIIVLEFCNLLRRDT 408

Query: 301  PEDSLYVLCRSTLGSLLETDGNSRGMRCLESVWGPIKTPELFPFVNELANKLSIDLSSSI 360
            PEDSLYVLCRSTLGSLLETDGNSRGMRCLESVWGPIKTPELFPFVNELANKLSIDLSSSI
Sbjct: 409  PEDSLYVLCRSTLGSLLETDGNSRGMRCLESVWGPIKTPELFPFVNELANKLSIDLSSSI 468

Query: 361  RSPTSAGPSXANKLSIELSSSIRSPTSAGPSVTDISDFTAFLLPLRNAIPEKLSFHGSKN 420
                                  RSPTSAGPSVTDISDFTAFLLPLRNAIPEKLSFHGSKN
Sbjct: 469  ----------------------RSPTSAGPSVTDISDFTAFLLPLRNAIPEKLSFHGSKN 528

Query: 421  VPLDRGGFRDSSYGEEGEFLHNIYLSLLKQMDICLQGMEAFLTDKGKGNCVIPYIGWSQY 480
            VPLDRG +RDSSYGEEGEFL NIYLSLLKQMDICLQGMEAFLTDKGKGNCVIPYIGWSQY
Sbjct: 529  VPLDRGDYRDSSYGEEGEFLLNIYLSLLKQMDICLQGMEAFLTDKGKGNCVIPYIGWSQY 588

Query: 481  LPILKELNDISLLFQGLKEEFWAIMRPRKSSICELIIRFAKRADDYSWILCHKDVINSES 540
            LPILKELNDISLLFQGLKEEFWAIMRPRKSSICELIIRFAKRADDYSWILCHKDVINSES
Sbjct: 589  LPILKELNDISLLFQGLKEEFWAIMRPRKSSICELIIRFAKRADDYSWILCHKDVINSES 648

Query: 541  RRHLSMLMFPEPTEDYEELQEMLIDRSQLLEESFEYITNASVEALRHGLFVLFKNEEATG 600
            RRHLSMLMFPEPTEDYEELQEMLIDRSQLLEESFEYITNASVEALRHGLFVLFKNEEATG
Sbjct: 649  RRHLSMLMFPEPTEDYEELQEMLIDRSQLLEESFEYITNASVEALRHGLFVLFKNEEATG 708

Query: 601  PGVLREWFLLVCKAIFNPQNALFVACPNDRRRFFPNPVSKVDPMHLSYFSFSGRVIALAL 660
            PGVLREWFLLVCKAIFNPQNALFVACPNDRRRFFPNPVSKVDPMHLSYFSFSGRVIALAL
Sbjct: 709  PGVLREWFLLVCKAIFNPQNALFVACPNDRRRFFPNPVSKVDPMHLSYFSFSGRVIALAL 768

Query: 661  MHNVQVGVVFDRVFFLQLAGMCISLEDIRDADPYLYGSCKQILDMDAELVDSDVLGLTFA 720
            MHNVQVGVVFDRVFFLQLAGMCISLEDIRDADPYLYGSCKQILDMDAELVDSDVLGLTFA
Sbjct: 769  MHNVQVGVVFDRVFFLQLAGMCISLEDIRDADPYLYGSCKQILDMDAELVDSDVLGLTFA 828

Query: 721  SDFEELGTREVVDLCPGGKDMVVNSKNRKEYVQLLVENRFMKSVSKQISYFANGFTDVLA 780
            SDFEELGTRE VDLCPGGKDMVVNSKNRKEYVQLL+ENRFMKSVSKQISYFANGFTDVLA
Sbjct: 829  SDFEELGTREFVDLCPGGKDMVVNSKNRKEYVQLLIENRFMKSVSKQISYFANGFTDVLA 888

Query: 781  DKRSHKCFFQSLELEDLDWMLYGSESQISVEDWKAHTEYSGYKETDPQISWFWKIVSAMT 840
            DKRSHKCFFQSLE EDLDWMLYGSESQISVEDWKAHTEYSGYKETDPQISWFWKIVSAMT
Sbjct: 889  DKRSHKCFFQSLEFEDLDWMLYGSESQISVEDWKAHTEYSGYKETDPQISWFWKIVSAMT 948

Query: 841  PEQRVNLLFFWTSVKYLPIQGFNGLASKLYIYKSSSPYDHLPSSHTCFFRLCFPPYPSEL 900
            PEQRVNLLFFWTSVKYLPIQGFNGLASKLYIYKSSSPYDHLPSSHTCFFRLCFPPYPSE 
Sbjct: 949  PEQRVNLLFFWTSVKYLPIQGFNGLASKLYIYKSSSPYDHLPSSHTCFFRLCFPPYPSES 1004

Query: 901  IMKRRLEIITQEHIGCSF 918
            IMKRRLEIITQEHIGCSF
Sbjct: 1009 IMKRRLEIITQEHIGCSF 1004

BLAST of Cp4.1LG10g10030.1 vs. ExPASy TrEMBL
Match: A0A6J1GF78 (E3 ubiquitin-protein ligase UPL5-like OS=Cucurbita moschata OX=3662 GN=LOC111453624 PE=4 SV=1)

HSP 1 Score: 1772 bits (4590), Expect = 0.0
Identity = 892/918 (97.17%), Postives = 893/918 (97.28%), Query Frame = 0

Query: 1   GTADAEHCLLNRHNSQGHRHPCVSSKRKLDDYAASLDDEDEDAPLFDLISIRMKKDETSA 60
           GTADAEHCLLNRHNSQGHRH CVSSKRKLDDYAASLDDEDEDAPLFDLISIRMKKDETSA
Sbjct: 18  GTADAEHCLLNRHNSQGHRHHCVSSKRKLDDYAASLDDEDEDAPLFDLISIRMKKDETSA 77

Query: 61  IDSSFDGRLGEGTSSDFEYRGFDDSSTSMAAETSLKPILSPHELQFFVRTMSVGNTMVML 120
           IDSSFDGRLGEGTSSDFEYRGFDDSSTSMAAETS KPILSPHELQFFVRTMSVGNTMVML
Sbjct: 78  IDSSFDGRLGEGTSSDFEYRGFDDSSTSMAAETSPKPILSPHELQFFVRTMSVGNTMVML 137

Query: 121 ANTNDTVMSLHERIQSITRIPVFEQRLIYRGRQLQHEQSLRECSIQNNAELQLVGRMRST 180
           ANTNDTVMSLHERIQSITRIPVFEQRLIYRGRQLQHEQSLRECSIQNNAELQLVGRMRST
Sbjct: 138 ANTNDTVMSLHERIQSITRIPVFEQRLIYRGRQLQHEQSLRECSIQNNAELQLVGRMRST 197

Query: 181 EHPKAWQIVDDMVSLVLRLYRGEVVFSASEILTTLMTDFLSLATQTDLDPAMKQLQVFLS 240
           EHPKAWQIVDDMVSLVLRLYRGEVVFSASEILTTLMTDFLSLATQTDLDPAMKQLQVFLS
Sbjct: 198 EHPKAWQIVDDMVSLVLRLYRGEVVFSASEILTTLMTDFLSLATQTDLDPAMKQLQVFLS 257

Query: 241 LSAPAALVMLYLSPIKGNTDCADNLIKHFMDLLCHSVPKSLKKCCAIIVLEFCNLLRRDT 300
           LSAPAALVMLYLSPIKGNTDCADNLIKHFMDLLCHSVPKSLKKCCAIIVLEFCNLLRRDT
Sbjct: 258 LSAPAALVMLYLSPIKGNTDCADNLIKHFMDLLCHSVPKSLKKCCAIIVLEFCNLLRRDT 317

Query: 301 PEDSLYVLCRSTLGSLLETDGNSRGMRCLESVWGPIKTPELFPFVNELANKLSIDLSSSI 360
           PEDSLYVLCRSTLGSLLETDGNSRGMRCLESVWGPIKTPELFPFVNELANKLSIDLSSSI
Sbjct: 318 PEDSLYVLCRSTLGSLLETDGNSRGMRCLESVWGPIKTPELFPFVNELANKLSIDLSSSI 377

Query: 361 RSPTSAGPSXANKLSIELSSSIRSPTSAGPSVTDISDFTAFLLPLRNAIPEKLSFHGSKN 420
                                 RSPTSAGPSVTDISDFTAFLLPLRNAIPEKLSFHGSKN
Sbjct: 378 ----------------------RSPTSAGPSVTDISDFTAFLLPLRNAIPEKLSFHGSKN 437

Query: 421 VPLDRGGFRDSSYGEEGEFLHNIYLSLLKQMDICLQGMEAFLTDKGKGNCVIPYIGWSQY 480
           VPLDRGGFRDSSYGEEGEFLHNIYLSLLKQMDICLQGMEAFLTDKGKGNCVIPYIGWSQY
Sbjct: 438 VPLDRGGFRDSSYGEEGEFLHNIYLSLLKQMDICLQGMEAFLTDKGKGNCVIPYIGWSQY 497

Query: 481 LPILKELNDISLLFQGLKEEFWAIMRPRKSSICELIIRFAKRADDYSWILCHKDVINSES 540
           LPILKELNDISLLFQGLKEEFWAIMRPRKSSICELIIRFAKRADDYSWILCHKDVINSES
Sbjct: 498 LPILKELNDISLLFQGLKEEFWAIMRPRKSSICELIIRFAKRADDYSWILCHKDVINSES 557

Query: 541 RRHLSMLMFPEPTEDYEELQEMLIDRSQLLEESFEYITNASVEALRHGLFVLFKNEEATG 600
           RRHLSMLMFPEPTEDYEELQEMLIDRSQLLEESFEYITNASVEALRHGLFVLFKNEEATG
Sbjct: 558 RRHLSMLMFPEPTEDYEELQEMLIDRSQLLEESFEYITNASVEALRHGLFVLFKNEEATG 617

Query: 601 PGVLREWFLLVCKAIFNPQNALFVACPNDRRRFFPNPVSKVDPMHLSYFSFSGRVIALAL 660
           PGVLREWFLLVCKAIFNPQNALFVACPNDRRRFFPNPVSKVDPMHLSYFSFSGRVIALAL
Sbjct: 618 PGVLREWFLLVCKAIFNPQNALFVACPNDRRRFFPNPVSKVDPMHLSYFSFSGRVIALAL 677

Query: 661 MHNVQVGVVFDRVFFLQLAGMCISLEDIRDADPYLYGSCKQILDMDAELVDSDVLGLTFA 720
           MHNVQVGVVFDRVFFLQLAGMCISLEDIRDADPYLYGSCKQILDMDAELVDSDVLGLTFA
Sbjct: 678 MHNVQVGVVFDRVFFLQLAGMCISLEDIRDADPYLYGSCKQILDMDAELVDSDVLGLTFA 737

Query: 721 SDFEELGTREVVDLCPGGKDMVVNSKNRKEYVQLLVENRFMKSVSKQISYFANGFTDVLA 780
           SDFEELGTREVVDLCPGGKDMVVNSKNRKEYVQLL+ENRFMKSVSKQISYFANGFTDVLA
Sbjct: 738 SDFEELGTREVVDLCPGGKDMVVNSKNRKEYVQLLIENRFMKSVSKQISYFANGFTDVLA 797

Query: 781 DKRSHKCFFQSLELEDLDWMLYGSESQISVEDWKAHTEYSGYKETDPQISWFWKIVSAMT 840
           DKRSHKCFFQSLELEDLDWMLYGSESQISVEDWKAHTEYSGYKETDPQISWFWKIVSAMT
Sbjct: 798 DKRSHKCFFQSLELEDLDWMLYGSESQISVEDWKAHTEYSGYKETDPQISWFWKIVSAMT 857

Query: 841 PEQRVNLLFFWTSVKYLPIQGFNGLASKLYIYKSSSPYDHLPSSHTCFFRLCFPPYPSEL 900
           PEQRVNLLFFWTSVKYLPIQGFNGLASKLYIYKSSSPYDHLPSSHTCFFRLCFPPYPSE 
Sbjct: 858 PEQRVNLLFFWTSVKYLPIQGFNGLASKLYIYKSSSPYDHLPSSHTCFFRLCFPPYPSES 913

Query: 901 IMKRRLEIITQEHIGCSF 918
           IMKRRLEIITQEHIGCSF
Sbjct: 918 IMKRRLEIITQEHIGCSF 913

BLAST of Cp4.1LG10g10030.1 vs. ExPASy TrEMBL
Match: A0A6J1ISB0 (E3 ubiquitin-protein ligase UPL5-like OS=Cucurbita maxima OX=3661 GN=LOC111478848 PE=4 SV=1)

HSP 1 Score: 1749 bits (4531), Expect = 0.0
Identity = 882/920 (95.87%), Postives = 888/920 (96.52%), Query Frame = 0

Query: 1   GTADAEHCLLNRHNSQGHRHPCVSSKRKLDDYAASLDDEDED--APLFDLISIRMKKDET 60
           GTADAEHC LNRHNSQGHRHPCVSSKRKLDDYAASLDDEDED  A LFDLISIRMKKDET
Sbjct: 18  GTADAEHCQLNRHNSQGHRHPCVSSKRKLDDYAASLDDEDEDEDAQLFDLISIRMKKDET 77

Query: 61  SAIDSSFDGRLGEGTSSDFEYRGFDDSSTSMAAETSLKPILSPHELQFFVRTMSVGNTMV 120
           SAIDSSFDGRLGEGTSSDFEYRGFDDSSTSMAAETS KPIL PHELQFFVRTMSVGNTMV
Sbjct: 78  SAIDSSFDGRLGEGTSSDFEYRGFDDSSTSMAAETSPKPILFPHELQFFVRTMSVGNTMV 137

Query: 121 MLANTNDTVMSLHERIQSITRIPVFEQRLIYRGRQLQHEQSLRECSIQNNAELQLVGRMR 180
           MLANTND+V+SLHERIQ+ITRIPVFEQRLIYRGRQLQHEQSLRECSIQNNAELQLVGRMR
Sbjct: 138 MLANTNDSVISLHERIQAITRIPVFEQRLIYRGRQLQHEQSLRECSIQNNAELQLVGRMR 197

Query: 181 STEHPKAWQIVDDMVSLVLRLYRGEVVFSASEILTTLMTDFLSLATQTDLDPAMKQLQVF 240
           STEHPKAWQIVDDMVSLVLRLYRGEVVFSASEILTTLMTDFLSLATQTDLDPAMKQLQVF
Sbjct: 198 STEHPKAWQIVDDMVSLVLRLYRGEVVFSASEILTTLMTDFLSLATQTDLDPAMKQLQVF 257

Query: 241 LSLSAPAALVMLYLSPIKGNTDCADNLIKHFMDLLCHSVPKSLKKCCAIIVLEFCNLLRR 300
           LSLSAPAALVMLYLSPIKGNTDCADNLIKHFMDLLCHSVPKSLKKCCAIIVLEFCNLLRR
Sbjct: 258 LSLSAPAALVMLYLSPIKGNTDCADNLIKHFMDLLCHSVPKSLKKCCAIIVLEFCNLLRR 317

Query: 301 DTPEDSLYVLCRSTLGSLLETDGNSRGMRCLESVWGPIKTPELFPFVNELANKLSIDLSS 360
           DTPED LYVLCRSTLGSLLETDGNSRGMRCLESVWGPIKTPELFPFVNELANKLSIDLSS
Sbjct: 318 DTPEDGLYVLCRSTLGSLLETDGNSRGMRCLESVWGPIKTPELFPFVNELANKLSIDLSS 377

Query: 361 SIRSPTSAGPSXANKLSIELSSSIRSPTSAGPSVTDISDFTAFLLPLRNAIPEKLSFHGS 420
           SI                      RSPT AGPSVTDISDFTAFLLPLRNAIPEKLSFHGS
Sbjct: 378 SI----------------------RSPTIAGPSVTDISDFTAFLLPLRNAIPEKLSFHGS 437

Query: 421 KNVPLDRGGFRDSSYGEEGEFLHNIYLSLLKQMDICLQGMEAFLTDKGKGNCVIPYIGWS 480
           KNVPLDRGGFRDSSYGEEGEFLHNIYLSLLKQMD+CLQGMEAFLTDKGKGNCVIPYIGWS
Sbjct: 438 KNVPLDRGGFRDSSYGEEGEFLHNIYLSLLKQMDLCLQGMEAFLTDKGKGNCVIPYIGWS 497

Query: 481 QYLPILKELNDISLLFQGLKEEFWAIMRPRKSSICELIIRFAKRADDYSWILCHKDVINS 540
           QYLPILKELNDISLLFQGLKEEFWAIMRPRKSSICELIIRFAKRADDYSWILCHKDVINS
Sbjct: 498 QYLPILKELNDISLLFQGLKEEFWAIMRPRKSSICELIIRFAKRADDYSWILCHKDVINS 557

Query: 541 ESRRHLSMLMFPEPTEDYEELQEMLIDRSQLLEESFEYITNASVEALRHGLFVLFKNEEA 600
           ESRRHLSMLMFPEPTEDYEELQEMLIDRSQLLEESFEYITNASVEALRHGLFVLFKNEEA
Sbjct: 558 ESRRHLSMLMFPEPTEDYEELQEMLIDRSQLLEESFEYITNASVEALRHGLFVLFKNEEA 617

Query: 601 TGPGVLREWFLLVCKAIFNPQNALFVACPNDRRRFFPNPVSKVDPMHLSYFSFSGRVIAL 660
           TGPGVLREWFLLVCKAIFNPQNALFVACPNDRRRFFPNPVSKVDPMHLSYFSFSGRVIAL
Sbjct: 618 TGPGVLREWFLLVCKAIFNPQNALFVACPNDRRRFFPNPVSKVDPMHLSYFSFSGRVIAL 677

Query: 661 ALMHNVQVGVVFDRVFFLQLAGMCISLEDIRDADPYLYGSCKQILDMDAELVDSDVLGLT 720
           ALMHNVQVGVVFDRVFFLQLAGMCISLEDIRDADPYLYGSCKQILDMDAELVDSDVLGLT
Sbjct: 678 ALMHNVQVGVVFDRVFFLQLAGMCISLEDIRDADPYLYGSCKQILDMDAELVDSDVLGLT 737

Query: 721 FASDFEELGTREVVDLCPGGKDMVVNSKNRKEYVQLLVENRFMKSVSKQISYFANGFTDV 780
           FASDFEELGTREVVDLCPGGKD+VVNSKNRKEYVQLL+ENRFMKSVSKQISYFANGFTDV
Sbjct: 738 FASDFEELGTREVVDLCPGGKDVVVNSKNRKEYVQLLIENRFMKSVSKQISYFANGFTDV 797

Query: 781 LADKRSHKCFFQSLELEDLDWMLYGSESQISVEDWKAHTEYSGYKETDPQISWFWKIVSA 840
           LADKRSHKCFFQSLELEDLDWMLYGSESQISVEDWKAHTEYSGYKETDPQISWFWKIVSA
Sbjct: 798 LADKRSHKCFFQSLELEDLDWMLYGSESQISVEDWKAHTEYSGYKETDPQISWFWKIVSA 857

Query: 841 MTPEQRVNLLFFWTSVKYLPIQGFNGLASKLYIYKSSSPYDHLPSSHTCFFRLCFPPYPS 900
           MTPEQRVNLLFFWTSVKYLPIQGFNGLASKLYIYKSSSPYDHLPSSHTCFFRLCFPPYPS
Sbjct: 858 MTPEQRVNLLFFWTSVKYLPIQGFNGLASKLYIYKSSSPYDHLPSSHTCFFRLCFPPYPS 915

Query: 901 ELIMKRRLEIITQEHIGCSF 918
           E IMKR LEIITQEHIGCSF
Sbjct: 918 ESIMKRCLEIITQEHIGCSF 915

BLAST of Cp4.1LG10g10030.1 vs. ExPASy TrEMBL
Match: A0A0A0KH74 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G511700 PE=4 SV=1)

HSP 1 Score: 1556 bits (4030), Expect = 0.0
Identity = 778/913 (85.21%), Postives = 831/913 (91.02%), Query Frame = 0

Query: 6   EHCLLNRHNSQGHRHPCVSSKRKLDDYAASLDDEDEDAPLFDLISIRMKKDETSAIDSSF 65
           EHC LNRH+SQGHRHPCV SKRKLDDY  S D++DED  LFDL+S RMKK ET A+DSSF
Sbjct: 30  EHCQLNRHHSQGHRHPCVLSKRKLDDYGPSFDEDDEDVHLFDLVSARMKKGETCAMDSSF 89

Query: 66  DGRLGEGTSSDFEYRGFDDSSTSMAAETSLKPILSPHELQFFVRTMSVGNTMVMLANTND 125
           DGRLGEGTSSDF++RGFDDSSTSMAAETS K ILSP++LQFFVRT+SVGNTMVMLAN ND
Sbjct: 90  DGRLGEGTSSDFDHRGFDDSSTSMAAETSTKSILSPYDLQFFVRTISVGNTMVMLANIND 149

Query: 126 TVMSLHERIQSITRIPVFEQRLIYRGRQLQHEQSLRECSIQNNAELQLVGRMRSTEHPKA 185
           TVMSLHERIQ+ITRIPVFEQRLIYRGRQLQHEQSL ECSIQNNAELQLVGRMRSTEHPKA
Sbjct: 150 TVMSLHERIQAITRIPVFEQRLIYRGRQLQHEQSLLECSIQNNAELQLVGRMRSTEHPKA 209

Query: 186 WQIVDDMVSLVLRLYRGEVVFSASEILTTLMTDFLSLATQTDLDPAMKQLQVFLSLSAPA 245
           WQIVDDMVSLVLRLYRGE VFSA EI+TTL+TDFL+LAT+ D DPA+KQLQVFLSLSAPA
Sbjct: 210 WQIVDDMVSLVLRLYRGEFVFSALEIITTLITDFLNLATEADSDPAIKQLQVFLSLSAPA 269

Query: 246 ALVMLYLSPIKGNTDCADNLIKHFMDLLCHSVPKSLKKCCAIIVLEFCNLLRRDTPEDSL 305
           ALVMLYLSPIKGN +CA+NLIKHFMDLL HS PKSL KCCAIIVLEFCNLLRRDTPEDSL
Sbjct: 270 ALVMLYLSPIKGNKECAENLIKHFMDLLRHSSPKSLHKCCAIIVLEFCNLLRRDTPEDSL 329

Query: 306 YVLCRSTLGSLLETDGNSRGMRCLESVWGPIKTPELFPFVNELANKLSIDLSSSIRSPTS 365
           YVLCRSTLGSLLETDG  RGMRCLESV GPIKTPELFPFV ELANKLSIDLSSSI+SPT+
Sbjct: 330 YVLCRSTLGSLLETDGIIRGMRCLESVRGPIKTPELFPFVTELANKLSIDLSSSIQSPTN 389

Query: 366 AGPSXANKLSIELSSSIRSPTSAGPSVTDISDFTAFLLPLRNAIPEKLSFHGSKNVPLDR 425
           A                     AGPSVTDI DFTAFLLPLRN I E+LSFHGSK VPL R
Sbjct: 390 A--------------------LAGPSVTDIRDFTAFLLPLRNVILEQLSFHGSKLVPLYR 449

Query: 426 GGFRDSSYGEEGEFLHNIYLSLLKQMDICLQGMEAFLTDKGKGNCVIPYIGWSQYLPILK 485
           GG RD SYGEEGEFLH+IY+ LLK+MD+CL GMEAFL DKGKG+CVIPYIGWSQYLPILK
Sbjct: 450 GGSRDPSYGEEGEFLHSIYIGLLKKMDMCLHGMEAFLIDKGKGDCVIPYIGWSQYLPILK 509

Query: 486 ELNDISLLFQGLKEEFWAIMRPRKSSICELIIRFAKRADDYSWILCHKDVINSESRRHLS 545
           ELN ISLLF+GL+EEFWAIMR RKSSI EL+IRFAKR DDY WILCHKD++NS SRRHLS
Sbjct: 510 ELNGISLLFEGLEEEFWAIMRSRKSSINELVIRFAKRTDDYLWILCHKDIMNSASRRHLS 569

Query: 546 MLMFPEPTEDYEELQEMLIDRSQLLEESFEYITNASVEALRHGLFVLFKNEEATGPGVLR 605
           MLMFPEPTEDYEELQEMLIDRSQLLEESFEYITNASVEALRHGLF+ FKNEEATGPGVLR
Sbjct: 570 MLMFPEPTEDYEELQEMLIDRSQLLEESFEYITNASVEALRHGLFMEFKNEEATGPGVLR 629

Query: 606 EWFLLVCKAIFNPQNALFVACPNDRRRFFPNPVSKVDPMHLSYFSFSGRVIALALMHNVQ 665
           EWFLLVCK+IFNPQNALFVACPNDRRRFFPNP SKVDPMHL+YF+FSGRVIALALM+ VQ
Sbjct: 630 EWFLLVCKSIFNPQNALFVACPNDRRRFFPNPASKVDPMHLNYFNFSGRVIALALMYKVQ 689

Query: 666 VGVVFDRVFFLQLAGMCISLEDIRDADPYLYGSCKQILDMDAELVDSDVLGLTFASDFEE 725
           VGVVFDRVFFLQLAGMCISLEDIRDADP LY SCKQILDMD  LVDSD LGLTF SDFEE
Sbjct: 690 VGVVFDRVFFLQLAGMCISLEDIRDADPCLYNSCKQILDMDPGLVDSDALGLTFVSDFEE 749

Query: 726 LGTREVVDLCPGGKDMVVNSKNRKEYVQLLVENRFMKSVSKQISYFANGFTDVLADKRSH 785
           LGTR+VVDLCPGGKDMVVNSKNR+EYV+LL+ENRFMKSVS+QISYFA+GFTD+L+ KR+H
Sbjct: 750 LGTRKVVDLCPGGKDMVVNSKNREEYVKLLIENRFMKSVSEQISYFASGFTDILSGKRTH 809

Query: 786 KCFFQSLELEDLDWMLYGSESQISVEDWKAHTEYSGYKETDPQISWFWKIVSAMTPEQRV 845
           KCFFQS+ELEDLDWMLYGSES ISV DWKAHTEY+GYKETDPQISWFWKIV  MTPEQR 
Sbjct: 810 KCFFQSIELEDLDWMLYGSESAISVGDWKAHTEYNGYKETDPQISWFWKIVYGMTPEQRK 869

Query: 846 NLLFFWTSVKYLPIQGFNGLASKLYIYKSSSPYDHLPSSHTCFFRLCFPPYPSELIMKRR 905
           NLLFFWTS+KYLP+QGF+GL SKLYIYKSSSPYDHLPSSHTCF+RLCFPPYPS  IMK R
Sbjct: 870 NLLFFWTSLKYLPVQGFSGLTSKLYIYKSSSPYDHLPSSHTCFYRLCFPPYPSRSIMKSR 922

Query: 906 LEIITQEHIGCSF 918
           L+IITQEH+GCSF
Sbjct: 930 LQIITQEHVGCSF 922

BLAST of Cp4.1LG10g10030.1 vs. ExPASy TrEMBL
Match: A0A1S3B104 (E3 ubiquitin-protein ligase UPL5 OS=Cucumis melo OX=3656 GN=LOC103484660 PE=4 SV=1)

HSP 1 Score: 1551 bits (4015), Expect = 0.0
Identity = 775/915 (84.70%), Postives = 832/915 (90.93%), Query Frame = 0

Query: 4   DAEHCLLNRHNSQGHRHPCVSSKRKLDDYAASLDDEDEDAPLFDLISIRMKKDETSAIDS 63
           D EHC LNRH+SQGHRHPCV SKRKLDDY  S D++DED  LFDL+S+RMKK ET A+DS
Sbjct: 28  DNEHCQLNRHHSQGHRHPCVLSKRKLDDYGPSFDEDDEDVHLFDLVSVRMKKGETCAMDS 87

Query: 64  SFDGRLGEGTSSDFEYRGFDDSSTSMAAETSLKPILSPHELQFFVRTMSVGNTMVMLANT 123
           SFDGRLGEGTSSDF++RGFDDSSTS+AAETS K IL P++LQFFVRT+SVGNTMVMLAN 
Sbjct: 88  SFDGRLGEGTSSDFDHRGFDDSSTSVAAETSTKSILYPYDLQFFVRTISVGNTMVMLANI 147

Query: 124 NDTVMSLHERIQSITRIPVFEQRLIYRGRQLQHEQSLRECSIQNNAELQLVGRMRSTEHP 183
           NDTVMSLHERIQ+ITRIPVFEQRLIYRGRQLQHEQSL ECSIQNNAELQLVGRMRSTEHP
Sbjct: 148 NDTVMSLHERIQAITRIPVFEQRLIYRGRQLQHEQSLLECSIQNNAELQLVGRMRSTEHP 207

Query: 184 KAWQIVDDMVSLVLRLYRGEVVFSASEILTTLMTDFLSLATQTDLDPAMKQLQVFLSLSA 243
           KAWQIVDDMVSLVLRLYRGE VFSA EI+TTL+TDFL+LAT+ DLDPA+KQLQVFLSLSA
Sbjct: 208 KAWQIVDDMVSLVLRLYRGEFVFSALEIITTLITDFLNLATEADLDPAIKQLQVFLSLSA 267

Query: 244 PAALVMLYLSPIKGNTDCADNLIKHFMDLLCHSVPKSLKKCCAIIVLEFCNLLRRDTPED 303
           PAALVMLYLSPIKGN +CA+NLIKHFMDLL HS PKSL K CAIIVLEFCNLLRRDTPED
Sbjct: 268 PAALVMLYLSPIKGNKECAENLIKHFMDLLRHSSPKSLHKFCAIIVLEFCNLLRRDTPED 327

Query: 304 SLYVLCRSTLGSLLETDGNSRGMRCLESVWGPIKTPELFPFVNELANKLSIDLSSSIRSP 363
           SLYVLCRSTLGSLLETDG  RGMRCLESV GPIKTPELFPFVNELANKLSIDLSSSI++P
Sbjct: 328 SLYVLCRSTLGSLLETDGIIRGMRCLESVRGPIKTPELFPFVNELANKLSIDLSSSIQAP 387

Query: 364 TSAGPSXANKLSIELSSSIRSPTSAGPSVTDISDFTAFLLPLRNAIPEKLSFHGSKNVPL 423
           T+A                     AGPSVTDI DFTAFLLPLRN I E+LSFH SK VPL
Sbjct: 388 TNA--------------------LAGPSVTDIRDFTAFLLPLRNVILEQLSFHRSKLVPL 447

Query: 424 DRGGFRDSSYGEEGEFLHNIYLSLLKQMDICLQGMEAFLTDKGKGNCVIPYIGWSQYLPI 483
            RGG RD SYGEEGEFLH+IY+ LLK+MD+CL GMEAFL DKGKG+CVIPYIGWSQYLPI
Sbjct: 448 YRGGSRDPSYGEEGEFLHSIYIGLLKKMDMCLHGMEAFLIDKGKGDCVIPYIGWSQYLPI 507

Query: 484 LKELNDISLLFQGLKEEFWAIMRPRKSSICELIIRFAKRADDYSWILCHKDVINSESRRH 543
           LKELN ISLLF+GL+EEFWAIMR RKSSI EL+IRFAKR DDY WILCHKD+I+S SRRH
Sbjct: 508 LKELNSISLLFEGLEEEFWAIMRSRKSSINELVIRFAKRTDDYMWILCHKDIIDSASRRH 567

Query: 544 LSMLMFPEPTEDYEELQEMLIDRSQLLEESFEYITNASVEALRHGLFVLFKNEEATGPGV 603
           LSMLMFPEPTEDYEELQEMLIDRS LLEESFEYITNASVEALRHGLF+ FKNEEATGPGV
Sbjct: 568 LSMLMFPEPTEDYEELQEMLIDRSHLLEESFEYITNASVEALRHGLFMEFKNEEATGPGV 627

Query: 604 LREWFLLVCKAIFNPQNALFVACPNDRRRFFPNPVSKVDPMHLSYFSFSGRVIALALMHN 663
           LREWFLLVCKAIFNPQNALFVACPNDRRRFFPNP SKVDPMHL+YF+FSGRVIALALM+ 
Sbjct: 628 LREWFLLVCKAIFNPQNALFVACPNDRRRFFPNPASKVDPMHLNYFNFSGRVIALALMYK 687

Query: 664 VQVGVVFDRVFFLQLAGMCISLEDIRDADPYLYGSCKQILDMDAELVDSDVLGLTFASDF 723
           VQVGVVFDRVFFLQLAGMCISLEDIRDADP LY SCKQILDMD ELVDSD LGLTF SDF
Sbjct: 688 VQVGVVFDRVFFLQLAGMCISLEDIRDADPCLYNSCKQILDMDPELVDSDALGLTFVSDF 747

Query: 724 EELGTREVVDLCPGGKDMVVNSKNRKEYVQLLVENRFMKSVSKQISYFANGFTDVLADKR 783
           EELGTR+VVDLCPGGKDMVVNSKNR++YV+LL+ENRFMKSVS+QISYFA+GFTD+L+ KR
Sbjct: 748 EELGTRKVVDLCPGGKDMVVNSKNREKYVKLLIENRFMKSVSEQISYFASGFTDILSGKR 807

Query: 784 SHKCFFQSLELEDLDWMLYGSESQISVEDWKAHTEYSGYKETDPQISWFWKIVSAMTPEQ 843
           +HKCFFQS+ELEDLDWMLYGSES ISV DWKAHTEY+GYKE+DPQISWFWKIV  MTPEQ
Sbjct: 808 THKCFFQSIELEDLDWMLYGSESAISVGDWKAHTEYNGYKESDPQISWFWKIVYGMTPEQ 867

Query: 844 RVNLLFFWTSVKYLPIQGFNGLASKLYIYKSSSPYDHLPSSHTCFFRLCFPPYPSELIMK 903
           R NLLFFWTS+KYLP+QGF+GL SKLYIYKSSSPYDHLPSSHTCF+RLCFPPYPS  IMK
Sbjct: 868 RKNLLFFWTSLKYLPVQGFSGLTSKLYIYKSSSPYDHLPSSHTCFYRLCFPPYPSRSIMK 922

Query: 904 RRLEIITQEHIGCSF 918
            RL+IITQEH+GCSF
Sbjct: 928 SRLQIITQEHVGCSF 922

BLAST of Cp4.1LG10g10030.1 vs. ExPASy TrEMBL
Match: A0A6J1BUW7 (E3 ubiquitin-protein ligase UPL5 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005576 PE=4 SV=1)

HSP 1 Score: 1473 bits (3814), Expect = 0.0
Identity = 744/914 (81.40%), Postives = 802/914 (87.75%), Query Frame = 0

Query: 5   AEHCLLNRHNSQGHRHPCVSSKRKLDDYAASLDDEDEDAPLFDLISIRMKKDETSAIDSS 64
           A+HC LNRH+SQGHRHPCVSSKRKLDDY  + DDEDED  LFDL+S+RMKK ET AIDSS
Sbjct: 26  ADHCQLNRHHSQGHRHPCVSSKRKLDDYGPNFDDEDEDVQLFDLVSVRMKKGETCAIDSS 85

Query: 65  FDGRLGEGTSSDFEYRGFDDSSTSMAAETSLKPILSPHELQFFVRTMSVGNTMVMLANTN 124
            DGRLGEG S D E+R FD    SMAAET  KPILSP+ELQFFVRT+S GNTMVMLAN N
Sbjct: 86  SDGRLGEGNSIDCEHRDFDTRPNSMAAETPQKPILSPYELQFFVRTISEGNTMVMLANIN 145

Query: 125 DTVMSLHERIQSITRIPVFEQRLIYRGRQLQHEQSLRECSIQNNAELQLVGRMRSTEHPK 184
           DTVMSLHERIQ ITRIPVFEQRLIYRGRQLQHEQSL ECSIQNNAELQLVGRMRSTEHPK
Sbjct: 146 DTVMSLHERIQGITRIPVFEQRLIYRGRQLQHEQSLLECSIQNNAELQLVGRMRSTEHPK 205

Query: 185 AWQIVDDMVSLVLRLYRGEVVFSASEILTTLMTDFLSLATQTDLDPAMKQLQVFLSLSAP 244
           AWQIVDDMVS VLRLYRGE VFSA E++TTLMT+FL LAT TDLD  +KQLQVFLSLSAP
Sbjct: 206 AWQIVDDMVSSVLRLYRGEFVFSALEVITTLMTEFLGLATDTDLDSGVKQLQVFLSLSAP 265

Query: 245 AALVMLYLSPIKGNTDCADNLIKHFMDLLCHSVPKSLKKCCAIIVLEFCNLLRRDTPEDS 304
           AALVML+LS IKGN +CAD LIKHFMDLL HSVPKSL  C AIIVLEFCNLLRRDTPE S
Sbjct: 266 AALVMLHLSKIKGNKECADKLIKHFMDLLRHSVPKSLHDCGAIIVLEFCNLLRRDTPEGS 325

Query: 305 LYVLCRSTLGSLLETDGNSRGMRCLESVWGPIKTPELFPFVNELANKLSIDLSSSIRSPT 364
           LY LCRSTLGSLL T+G SRGMRCLESV GPIKTPELFPFVNELANKLSIDLSSS+    
Sbjct: 326 LYSLCRSTLGSLLGTEGISRGMRCLESVRGPIKTPELFPFVNELANKLSIDLSSSM---- 385

Query: 365 SAGPSXANKLSIELSSSIRSPTSAGPSVTDISDFTAFLLPLRNAIPEKLSFHGSKNVPLD 424
                             RSPTSAGPSVTDI DFTAFLLPLR+A+ ++L F GSK +P  
Sbjct: 386 ------------------RSPTSAGPSVTDIRDFTAFLLPLRSAVMDQLGFLGSKFLPPY 445

Query: 425 RGGFRDSSYGEEGEFLHNIYLSLLKQMDICLQGMEAFLTDKGKGNCVIPYIGWSQYLPIL 484
           RG FRD SYGEEGEFLHNIY SLLK+MDICLQGMEAFL DKG  +C   Y GWSQYLPIL
Sbjct: 446 RGSFRDPSYGEEGEFLHNIYQSLLKKMDICLQGMEAFLVDKGNRDCANLYAGWSQYLPIL 505

Query: 485 KELNDISLLFQGLKEEFWAIMRPRKSSICELIIRFAKRADDYSWILCHKDVINSESRRHL 544
           KELN ISLLFQG +EEFWA MRPRK+SIC L+IRFAKRAD++ WIL HK+V NSESRRHL
Sbjct: 506 KELNSISLLFQGAEEEFWATMRPRKNSICALLIRFAKRADNHMWILQHKEVTNSESRRHL 565

Query: 545 SMLMFPEPTEDYEELQEMLIDRSQLLEESFEYITNASVEALRHGLFVLFKNEEATGPGVL 604
           +MLMFPEPTEDY+ELQEMLIDRSQLLEESFEYIT+A+VEALRHGLF+ FKNEEATGPGVL
Sbjct: 566 TMLMFPEPTEDYDELQEMLIDRSQLLEESFEYITHANVEALRHGLFMEFKNEEATGPGVL 625

Query: 605 REWFLLVCKAIFNPQNALFVACPNDRRRFFPNPVSKVDPMHLSYFSFSGRVIALALMHNV 664
           REWF LVC+AIFNPQNALFVACPNDRRRFFPNP SKVDP+HL YF+FSGRVIALALMH V
Sbjct: 626 REWFFLVCQAIFNPQNALFVACPNDRRRFFPNPASKVDPLHLKYFNFSGRVIALALMHKV 685

Query: 665 QVGVVFDRVFFLQLAGMCISLEDIRDADPYLYGSCKQILDMDAELVDSDVLGLTFASDFE 724
           QVG+VFDRVFFLQL+G+ ISLEDIRDADPYLY SCKQILDMDAE VDSD LGLTF SDFE
Sbjct: 686 QVGIVFDRVFFLQLSGISISLEDIRDADPYLYTSCKQILDMDAEFVDSDALGLTFVSDFE 745

Query: 725 ELGTREVVDLCPGGKDMVVNSKNRKEYVQLLVENRFMKSVSKQISYFANGFTDVLADKRS 784
           ELG R+VVDLCPGGK+MVVNSKNRKEYV+LLVENRF++SVS+Q+S FA+GFTDVL+D+R 
Sbjct: 746 ELGIRKVVDLCPGGKNMVVNSKNRKEYVKLLVENRFVRSVSEQVSCFASGFTDVLSDQRV 805

Query: 785 HKCFFQSLELEDLDWMLYGSESQISVEDWKAHTEYSGYKETDPQISWFWKIVSAMTPEQR 844
           H  FFQSLE+EDLDWMLYGSES ISV DWK HTEY+GYKETDPQISWFWKIV  MT EQR
Sbjct: 806 HTHFFQSLEIEDLDWMLYGSESAISVGDWKVHTEYNGYKETDPQISWFWKIVYGMTLEQR 865

Query: 845 VNLLFFWTSVKYLPIQGFNGLASKLYIYKSSSPYDHLPSSHTCFFRLCFPPYPSELIMKR 904
            NLLFFWTSVKYLP+QGF+GLASKLYIYKSSSPYD+LPSSHTCFFRLCFPPYPS+ IMK 
Sbjct: 866 KNLLFFWTSVKYLPVQGFSGLASKLYIYKSSSPYDYLPSSHTCFFRLCFPPYPSKAIMKS 917

Query: 905 RLEIITQEHIGCSF 918
           RL+IITQEH+GCSF
Sbjct: 926 RLQIITQEHVGCSF 917

BLAST of Cp4.1LG10g10030.1 vs. TAIR 10
Match: AT4G12570.1 (ubiquitin protein ligase 5 )

HSP 1 Score: 766.5 bits (1978), Expect = 2.9e-221
Identity = 410/840 (48.81%), Postives = 543/840 (64.64%), Query Frame = 0

Query: 81  GFDDSSTSMAAETSLKPILSPHELQFFVRTMSVGNTMVMLANTNDTVMSLHERIQSITRI 140
           G +  S S + E S         LQ FVR MS G T+V+ A   DTV  LH+RI+  T+I
Sbjct: 72  GENSRSLSSSGECSSSNRPESTRLQIFVRMMSGGKTIVIHAEKYDTVEKLHQRIEWKTKI 131

Query: 141 PVFEQRLIYRGRQLQHEQSLRECSIQNNAELQLVGRMRSTEHPKAWQIVDDMVSLVLRLY 200
           P  EQR+IY+G+QLQ E SL   SI+ +A LQLV RM+STEHP AWQ +DD++  + R+Y
Sbjct: 132 PALEQRVIYKGKQLQRENSLTYYSIEQDASLQLVARMQSTEHPVAWQTIDDIMYTISRMY 191

Query: 201 RGEVVFSASEILTTLMTDFLSLATQTDLDPAMKQLQVFLSLSAPAALVMLYLSPIKGNTD 260
           +GE +   S I   ++T F  +  ++D +   K L +F + S PAALVMLY S ++ N  
Sbjct: 192 KGENL--QSNINEKIVTFFAMIPVESD-ESIAKYLNIFSNSSVPAALVMLYASSLERNKS 251

Query: 261 CADNLIKHFMDLLCHSVPKSLKKCCAIIVLEFCNLLRRDTPEDSLYVLCRSTLGSLLETD 320
           CA + +K F+   C ++PK+ K  C  IVLEFC LLR+  P+  LYV CR+TLGS+LET 
Sbjct: 252 CAKSSVKLFLS-NCVALPKNQKNYCLPIVLEFCKLLRKVCPDQKLYVTCRNTLGSMLETF 311

Query: 321 GNSRGM-RCLESVWGPIKTPELFPFVNELANKLSIDLSSSIRSPTSAGPSXANKLSIELS 380
            N  G+       +G     E+FPF  EL   L  +L+                      
Sbjct: 312 DNPHGVYNDQYETFG----VEIFPFFTELTGLLLNELAQ--------------------- 371

Query: 381 SSIRSPTSAGPSVTDISDFTAFLLPLRNAIPEKLSFHGSKNVPLDRGGFRDSSYGEEGEF 440
                  ++GPS  D    ++F   LR  I  K++F     +P+     + ++   E   
Sbjct: 372 -------NSGPSFCDFQKVSSFWQQLRKVIELKVAFPIPIVLPM-----QSTALEAEIRH 431

Query: 441 LHNIYLSLLKQMDICLQGMEAFLTDKGKGNCVIPYIGWSQYLPILKELNDISLLFQGLKE 500
           LH ++ SLL  MD+C+  +E+ L DK  GN       WSQYL ILK +N +S ++QG K 
Sbjct: 432 LHRLFGSLLTTMDLCMCRVESSLADKEVGNSETMSSSWSQYLSILKIINSMSNIYQGAKG 491

Query: 501 EFWAIMRPRKSSICELIIRFAKRADDYSWILCHKDVINSESRRHLSMLMFPEPTEDYEEL 560
           +   ++   K S   L+++FAKR DD+ WI  +K+  N E+RRHL+ML+FP+  ED+EE+
Sbjct: 492 QLAVMLNKNKVSFSALVVKFAKRGDDHQWIFEYKEATNFEARRHLAMLLFPDVKEDFEEM 551

Query: 561 QEMLIDRSQLLEESFEYITNASVEALRHGLFVLFKNEEATGPGVLREWFLLVCKAIFNPQ 620
            EMLIDRS LL ESFEYI  AS EAL  GLF+ FKNEEATGPGVLREWF LVC+ IFNP+
Sbjct: 552 HEMLIDRSNLLSESFEYIVGASPEALHGGLFMEFKNEEATGPGVLREWFYLVCQEIFNPK 611

Query: 621 NALFVACPNDRRRFFPNPVSKVDPMHLSYFSFSGRVIALALMHNVQVGVVFDRVFFLQLA 680
           N LF+   +D RRF PNP SKVDP+H  +F F+GRVIALALMH VQVGV+FDRVFFLQLA
Sbjct: 612 NTLFLRSADDFRRFSPNPASKVDPLHPDFFEFTGRVIALALMHKVQVGVLFDRVFFLQLA 671

Query: 681 GMCISLEDIRDADPYLYGSCKQILDMDAELVDSDV-LGLTFASDFEELGTREVVDLCPGG 740
           G+ ISLEDI+D D  +Y SCKQIL+MD E  DS+  LGLTF  + EELG R+ ++LCP G
Sbjct: 672 GLKISLEDIKDTDRIMYNSCKQILEMDPEFFDSNAGLGLTFVLETEELGKRDTIELCPDG 731

Query: 741 KDMVVNSKNRKEYVQLLVENRFMKSVSKQISYFANGFTDVLADKRSHKCFFQSLELEDLD 800
           K   VNSKNRK+YV LL+E RF   + +Q+  F+ GFTD+L+     + FF+ L LEDLD
Sbjct: 732 KLKAVNSKNRKQYVDLLIERRFATPILEQVKQFSRGFTDMLSHSVPPRSFFKRLYLEDLD 791

Query: 801 WMLYGSESQISVEDWKAHTEYSGYKETDPQISWFWKIVSAMTPEQRVNLLFFWTSVKYLP 860
            ML G E+ IS++DWKAHTEY+G+KETD QI WFWKI+  MT E++ ++LFFWTS K++P
Sbjct: 792 GMLRGGENPISIDDWKAHTEYNGFKETDRQIDWFWKILKKMTEEEQRSILFFWTSNKFVP 851

Query: 861 IQGFNGLASKLYIYKSSSPYDHLPSSHTCFFRLCFPPYPSELIMKRRLEIITQEHIGCSF 919
           ++GF GL+SKLYIY+     D LP SHTCF+RLC P YP+  +M++RL +I Q+H+  SF
Sbjct: 852 VEGFRGLSSKLYIYRLYEANDRLPLSHTCFYRLCIPRYPTITLMEQRLRLIAQDHVSSSF 870

BLAST of Cp4.1LG10g10030.1 vs. TAIR 10
Match: AT1G55860.1 (ubiquitin-protein ligase 1 )

HSP 1 Score: 189.1 bits (479), Expect = 1.9e-47
Identity = 123/362 (33.98%), Postives = 184/362 (50.83%), Query Frame = 0

Query: 564  IDRSQLLEESFEYITNASVEALRHGLFVLFKNEEATGPGVL-REWFLLVCKAIFNPQNAL 623
            + R+ +LE+S+  +   S + L+  L V F+ EE    G L REW+ L+ + IF+    L
Sbjct: 3572 VRRAYVLEDSYNQLRMRSPQDLKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGALL 3631

Query: 624  FVACPNDRRRFFPNPVSKVDPMHLSYFSFSGRVIALALMHNVQVGVVFDRVFFLQLAGMC 683
            F    ND   F PNP S     HLSYF F GR++A AL     + V F R F+  + G+ 
Sbjct: 3632 FTTVGND-ATFQPNPNSVYQTEHLSYFKFVGRMVAKALFDGQLLDVYFTRSFYKHILGVK 3691

Query: 684  ISLEDIRDADPYLYGSCKQILDMDAELVDSDVLGLTFASDFEE-----LGTREVVD--LC 743
            ++  DI   DP  Y + K +L+ D     SD+L LTF+ D +E         EV D  L 
Sbjct: 3692 VTYHDIEAVDPDYYKNLKWLLENDV----SDILDLTFSMDADEEKHILYEKTEVTDYELK 3751

Query: 744  PGGKDMVVNSKNRKEYVQLLVENRFMKSVSKQISYFANGFTDVLADKRSHKCFFQSLELE 803
            PGG+++ V  + + EYV L+  +    ++  QI+ F  GF +++   R     F   ELE
Sbjct: 3752 PGGRNIRVTEETKHEYVDLVAGHILTNAIRPQINAFLEGFNELI--PRELVSIFNDKELE 3811

Query: 804  DLDWMLYGSESQISVEDWKAHTEYSGYKETDPQISWFWKIVSAMTPEQRVNLLFFWTSVK 863
                +L     +I  +D KA+TEY+ Y    P I WFW++V A + E     L F T   
Sbjct: 3812 ----LLISGLPEIDFDDLKANTEYTSYTAGSPVIHWFWEVVKAFSKEDMARFLQFVTGTS 3871

Query: 864  YLPIQGFNGL-----ASKLYIYKSSSPYDHLPSSHTCFFRLCFPPYPSELIMKRRLEIIT 913
             +P++GF  L       +L I+K+    + LPS+HTCF +L  P Y S+  ++ RL +  
Sbjct: 3872 KVPLEGFKALQGISGPQRLQIHKAYGAPERLPSAHTCFNQLDLPEYQSKEQLQERLLLAI 3922

BLAST of Cp4.1LG10g10030.1 vs. TAIR 10
Match: AT1G55860.2 (ubiquitin-protein ligase 1 )

HSP 1 Score: 189.1 bits (479), Expect = 1.9e-47
Identity = 123/362 (33.98%), Postives = 184/362 (50.83%), Query Frame = 0

Query: 564  IDRSQLLEESFEYITNASVEALRHGLFVLFKNEEATGPGVL-REWFLLVCKAIFNPQNAL 623
            + R+ +LE+S+  +   S + L+  L V F+ EE    G L REW+ L+ + IF+    L
Sbjct: 3323 VRRAYVLEDSYNQLRMRSPQDLKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGALL 3382

Query: 624  FVACPNDRRRFFPNPVSKVDPMHLSYFSFSGRVIALALMHNVQVGVVFDRVFFLQLAGMC 683
            F    ND   F PNP S     HLSYF F GR++A AL     + V F R F+  + G+ 
Sbjct: 3383 FTTVGND-ATFQPNPNSVYQTEHLSYFKFVGRMVAKALFDGQLLDVYFTRSFYKHILGVK 3442

Query: 684  ISLEDIRDADPYLYGSCKQILDMDAELVDSDVLGLTFASDFEE-----LGTREVVD--LC 743
            ++  DI   DP  Y + K +L+ D     SD+L LTF+ D +E         EV D  L 
Sbjct: 3443 VTYHDIEAVDPDYYKNLKWLLENDV----SDILDLTFSMDADEEKHILYEKTEVTDYELK 3502

Query: 744  PGGKDMVVNSKNRKEYVQLLVENRFMKSVSKQISYFANGFTDVLADKRSHKCFFQSLELE 803
            PGG+++ V  + + EYV L+  +    ++  QI+ F  GF +++   R     F   ELE
Sbjct: 3503 PGGRNIRVTEETKHEYVDLVAGHILTNAIRPQINAFLEGFNELI--PRELVSIFNDKELE 3562

Query: 804  DLDWMLYGSESQISVEDWKAHTEYSGYKETDPQISWFWKIVSAMTPEQRVNLLFFWTSVK 863
                +L     +I  +D KA+TEY+ Y    P I WFW++V A + E     L F T   
Sbjct: 3563 ----LLISGLPEIDFDDLKANTEYTSYTAGSPVIHWFWEVVKAFSKEDMARFLQFVTGTS 3622

Query: 864  YLPIQGFNGL-----ASKLYIYKSSSPYDHLPSSHTCFFRLCFPPYPSELIMKRRLEIIT 913
             +P++GF  L       +L I+K+    + LPS+HTCF +L  P Y S+  ++ RL +  
Sbjct: 3623 KVPLEGFKALQGISGPQRLQIHKAYGAPERLPSAHTCFNQLDLPEYQSKEQLQERLLLAI 3673

BLAST of Cp4.1LG10g10030.1 vs. TAIR 10
Match: AT1G70320.1 (ubiquitin-protein ligase 2 )

HSP 1 Score: 188.3 bits (477), Expect = 3.2e-47
Identity = 125/369 (33.88%), Postives = 187/369 (50.68%), Query Frame = 0

Query: 564  IDRSQLLEESFEYITNASVEALRHGLFVLFKNEEATGPGVL-REWFLLVCKAIFNPQNAL 623
            + R+ +LE+S+  +   S + L+  L V F+ EE    G L REW+ L+ + IF+    L
Sbjct: 3300 VRRAYVLEDSYNQLRMRSPQDLKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGALL 3359

Query: 624  FVACPNDRRRFFPNPVSKVDPMHLSYFSFSGRVIALALMHNVQVGVVFDRVFFLQLAGMC 683
            F    ND   F PNP S     HLSYF F GR++A AL     + V F R F+  + G+ 
Sbjct: 3360 FTTVGND-ATFQPNPNSVYQTEHLSYFKFVGRMVAKALFDGQLLDVYFTRSFYKHILGVK 3419

Query: 684  ISLEDIRDADPYLYGSCKQILDMDAELVDSDVLGLTFASDFEE-----LGTREVVD--LC 743
            ++  DI   DP  Y + K +L+ D     SD+L LTF+ D +E         EV D  L 
Sbjct: 3420 VTYHDIEAVDPDYYKNLKWLLENDV----SDILDLTFSMDADEEKHILYEKTEVTDYELK 3479

Query: 744  PGGKDMVVNSKNRKEYVQLLVENRFMKSVSKQISYFANGFTDVLADKRSHKCFFQSLELE 803
            PGG+++ V  + + EYV L+ ++    ++  QI+ F  G  +++   R     F   ELE
Sbjct: 3480 PGGRNIRVTEETKHEYVDLVADHILTSAIRPQINAFLEGLNELI--PRELVSIFNDKELE 3539

Query: 804  DLDWMLYGSESQISVEDWKAHTEYSGYKETDPQISWFWKIVSAMTPEQRVNLLFFWTSVK 863
                +L     +I  +D KA+TEY+ Y    P I WFW++V A + E     L F T   
Sbjct: 3540 ----LLISGLPEIDFDDLKANTEYTSYTVGSPVIRWFWEVVKAFSKEDMARFLQFVTGTS 3599

Query: 864  YLPIQGFNGL-----ASKLYIYKSSSPYDHLPSSHTCFFRLCFPPYPS-ELIMKRRLEII 919
             +P++GF  L       +L I+K+    + LPS+HTCF +L  P Y S E + +R L  I
Sbjct: 3600 KVPLEGFKALQGISGPQRLQIHKAYGSPERLPSAHTCFNQLDLPEYQSKEQVQERLLLAI 3657

BLAST of Cp4.1LG10g10030.1 vs. TAIR 10
Match: AT3G53090.1 (ubiquitin-protein ligase 7 )

HSP 1 Score: 135.6 bits (340), Expect = 2.5e-31
Identity = 91/364 (25.00%), Postives = 164/364 (45.05%), Query Frame = 0

Query: 561  EMLIDRSQLLEESFEYITNASVEALRHGLFVLFKNEEATGP------GVLREWFLLVCKA 620
            E+++ R  ++E+ F+ + N+    L+  + V F NE           G+ +E+   + KA
Sbjct: 775  EIVVRRGHVVEDGFQQL-NSIGSRLKSSIHVSFVNESGLPEAGLDYGGLSKEFLTDITKA 834

Query: 621  IFNPQNALFVACPNDRRRFFPNPVSKVDPMHLSYFSFSGRVIALALMHNVQVGVVFDRVF 680
             F  +  LF   P   R   P+P ++     +    F GR++  AL   + +   F  VF
Sbjct: 835  AFATEYGLFSQTPTSDRLLVPSPSARHLENGIQMIEFLGRIVGKALYEGILLDYSFSHVF 894

Query: 681  FLQLAGMCISLEDIRDADPYLYGSCKQILDMDAELVDSDVLGLTFASDFEELGTREVVDL 740
              +L G    ++++   DP LY +   +   D +L +   L L F    E  G   +++L
Sbjct: 895  IQKLLGRYSFIDELSGLDPELYRNLMYVKHYDGDLKE---LCLDFTVTEEFCGKMSIIEL 954

Query: 741  CPGGKDMVVNSKNRKEYVQLLVENRFMKSVSKQISYFANGFTDVLADKRSHKCFFQSLEL 800
             PGGKD  V ++N+ +Y+  + + +  + +    + F  G TD+++       + +    
Sbjct: 955  KPGGKDTSVTNENKMQYIHAMADYKLNRQIVPFSNAFYRGLTDLIS-----PAWLKLFNA 1014

Query: 801  EDLDWMLYGSESQISVEDWKAHTEYS-GYKETDPQISWFWKIVSAMTPEQRVNLLFFWTS 860
             + + +L G    I V+D + +T+Y+ GY ++   I  FW+++    P +R  LL F TS
Sbjct: 1015 HEFNQLLSGGNHDIDVDDLRRNTKYTGGYSDSSRTIKIFWEVMKGFEPSERCLLLKFVTS 1074

Query: 861  VKYLPIQGFNGLASKLYIYKSS-----------SPYDHLPSSHTCFFRLCFPPYPSELIM 907
                P+ GF  L     I+K S              + LPS+ TC+  L  P Y     M
Sbjct: 1075 CSRAPLLGFKYLQPTFIIHKVSCDTSLWAAIGGQDVERLPSASTCYNTLKLPTYKRASTM 1129