Cp4.1LG10g06420.1 (mRNA) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG10g06420.1
Type	mRNA
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	alpha/beta-Hydrolases superfamily protein
Location	Cp4.1LG10: 320524 .. 321510 (+)
Sequence length	987
RNA-Seq Expression	Cp4.1LG10g06420.1
Synteny	Cp4.1LG10g06420.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGCACCCAATAAACCAGGCCAACGAGAACAGCCCCTATGGAGGCCTAACCAGGGAACAGTTCTACAAGAAACACAATGTTACTCACCATGAAGCCTTCATTCTCAATGCCCAGGAGATGAAAATCTTCACTCAGTCATGGCGACCTGTTTCTGACTCAGAGCTCAAAGGGGTTGTCGCCATGGTCCATGGCTACACCTCTGACAGTGGCTGGATGTTTGAGCTCACTGCCGTTGCCATAGCCAAAGCTGGGTTCCTTGTTTGCTCTCTCGACCTTCAAGGCCATGGCCGATCCGACGGCGCCCCTGGTTCGATCCCAGACATCGAACTACTCGTTCTCGACTGTACTCTGTTTTTCGACTCTGTTCGAGAACAGAATCCTACCCTCCCTGCTTTTCTGTACGGGGAATCGCTAGGCGGTGCGATTTCGATCCTGATTTGCTTGAAGCAAGAGGGGGTTTGGAGCGGAATCGTGTTAAACGGCTCCATGTGTGGAATCTCGGCGAAATTCAAACCGATTTGGCCATTGGAGAAGCTTCTTCCAATAGCAGCGTCTTTAGCGCCGTCGCTGCGATTGGTGTTATCAAAGCCCCACGCAAGCAAGTCGTACAAGGAGGAGTGGAAGAGGAGATTGGTGGCGAAAAACCCGAACCGACGATTTTCAGGAAAGCCACCAATGGCGACAGCATTGGAGTTTCTCAGAGTGTGCGAGTACATAAAAATGAACTGCCATGAAATCAGGGTTCCAATGTTGATGGTTCACGGGGAAGACGATGTGGTTTGCGACAGCGAGTCGGCGAGATTTGTGTTCGAATCGGCGGCGAGTAAGGACAAGACGTTGAAGGTTTATCCAGGAATGTGGCACCAGTTGATAGGCGAGGCGAATGAGAGTGTGGAGATTGTTTATACCACCATTTTCAACTGGTTAGTGGATAGAGCAGAGAAGGCCAAGGCCAAGAACAAGAACAAGAACACCAAAAAGTGA

mRNA sequence

ATGGCGCACCCAATAAACCAGGCCAACGAGAACAGCCCCTATGGAGGCCTAACCAGGGAACAGTTCTACAAGAAACACAATGTTACTCACCATGAAGCCTTCATTCTCAATGCCCAGGAGATGAAAATCTTCACTCAGTCATGGCGACCTGTTTCTGACTCAGAGCTCAAAGGGGTTGTCGCCATGGTCCATGGCTACACCTCTGACAGTGGCTGGATGTTTGAGCTCACTGCCGTTGCCATAGCCAAAGCTGGGTTCCTTGTTTGCTCTCTCGACCTTCAAGGCCATGGCCGATCCGACGGCGCCCCTGGTTCGATCCCAGACATCGAACTACTCGTTCTCGACTGTACTCTGTTTTTCGACTCTGTTCGAGAACAGAATCCTACCCTCCCTGCTTTTCTGTACGGGGAATCGCTAGGCGGTGCGATTTCGATCCTGATTTGCTTGAAGCAAGAGGGGGTTTGGAGCGGAATCGTGTTAAACGGCTCCATGTGTGGAATCTCGGCGAAATTCAAACCGATTTGGCCATTGGAGAAGCTTCTTCCAATAGCAGCGTCTTTAGCGCCGTCGCTGCGATTGGTGTTATCAAAGCCCCACGCAAGCAAGTCGTACAAGGAGGAGTGGAAGAGGAGATTGGTGGCGAAAAACCCGAACCGACGATTTTCAGGAAAGCCACCAATGGCGACAGCATTGGAGTTTCTCAGAGTGTGCGAGTACATAAAAATGAACTGCCATGAAATCAGGGTTCCAATGTTGATGGTTCACGGGGAAGACGATGTGGTTTGCGACAGCGAGTCGGCGAGATTTGTGTTCGAATCGGCGGCGAGTAAGGACAAGACGTTGAAGGTTTATCCAGGAATGTGGCACCAGTTGATAGGCGAGGCGAATGAGAGTGTGGAGATTGTTTATACCACCATTTTCAACTGGTTAGTGGATAGAGCAGAGAAGGCCAAGGCCAAGAACAAGAACAAGAACACCAAAAAGTGA

Coding sequence (CDS)

ATGGCGCACCCAATAAACCAGGCCAACGAGAACAGCCCCTATGGAGGCCTAACCAGGGAACAGTTCTACAAGAAACACAATGTTACTCACCATGAAGCCTTCATTCTCAATGCCCAGGAGATGAAAATCTTCACTCAGTCATGGCGACCTGTTTCTGACTCAGAGCTCAAAGGGGTTGTCGCCATGGTCCATGGCTACACCTCTGACAGTGGCTGGATGTTTGAGCTCACTGCCGTTGCCATAGCCAAAGCTGGGTTCCTTGTTTGCTCTCTCGACCTTCAAGGCCATGGCCGATCCGACGGCGCCCCTGGTTCGATCCCAGACATCGAACTACTCGTTCTCGACTGTACTCTGTTTTTCGACTCTGTTCGAGAACAGAATCCTACCCTCCCTGCTTTTCTGTACGGGGAATCGCTAGGCGGTGCGATTTCGATCCTGATTTGCTTGAAGCAAGAGGGGGTTTGGAGCGGAATCGTGTTAAACGGCTCCATGTGTGGAATCTCGGCGAAATTCAAACCGATTTGGCCATTGGAGAAGCTTCTTCCAATAGCAGCGTCTTTAGCGCCGTCGCTGCGATTGGTGTTATCAAAGCCCCACGCAAGCAAGTCGTACAAGGAGGAGTGGAAGAGGAGATTGGTGGCGAAAAACCCGAACCGACGATTTTCAGGAAAGCCACCAATGGCGACAGCATTGGAGTTTCTCAGAGTGTGCGAGTACATAAAAATGAACTGCCATGAAATCAGGGTTCCAATGTTGATGGTTCACGGGGAAGACGATGTGGTTTGCGACAGCGAGTCGGCGAGATTTGTGTTCGAATCGGCGGCGAGTAAGGACAAGACGTTGAAGGTTTATCCAGGAATGTGGCACCAGTTGATAGGCGAGGCGAATGAGAGTGTGGAGATTGTTTATACCACCATTTTCAACTGGTTAGTGGATAGAGCAGAGAAGGCCAAGGCCAAGAACAAGAACAAGAACACCAAAAAGTGA

Protein sequence

MAHPINQANENSPYGGLTREQFYKKHNVTHHEAFILNAQEMKIFTQSWRPVSDSELKGVVAMVHGYTSDSGWMFELTAVAIAKAGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFFDSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFKPIWPLEKLLPIAASLAPSLRLVLSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYIKMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASKDKTLKVYPGMWHQLIGEANESVEIVYTTIFNWLVDRAEKAKAKNKNKNTKK

Homology

[Close]

BLAST of Cp4.1LG10g06420.1 vs. ExPASy Swiss-Prot
Match: Q9C942 (Caffeoylshikimate esterase OS=Arabidopsis thaliana OX=3702 GN=CSE PE=1 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 7.8e-47
Identity = 106/311 (34.08%), Postives = 175/311 (56.27%), Query Frame = 0


Query: 14  YGGLTREQFYKKHNVTHHEAFILNAQEMKIFTQSWRPVSDSELKGVVAMVHGYTSDSGWM 73
           +G +  E++Y    V + +++       K+FTQS+ P+ D E+KG V M HGY SD+ WM
Sbjct: 23  WGTMPEEEYYTSQGVRNSKSY-FETPNGKLFTQSFLPL-DGEIKGTVYMSHGYGSDTSWM 82

Query: 74  FELTAVAIAKAGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFFDSVREQNP--TLP 133
           F+   ++ +  G+ V + DL GHGRSDG    + D+E +      FF  VR  +P   LP
Sbjct: 83  FQKICMSFSSWGYAVFAADLLGHGRSDGIRCYMGDMEKVAATSLAFFKHVRCSDPYKDLP 142

Query: 134 AFLYGESLGGAISILICLKQE-GVWSGIVLNGSMCGISAKFKPIWPLEKLLPIAASLAPS 193
           AFL+GES+GG +++L+  + E   W+G++ +  +  I    KP         +   LA +
Sbjct: 143 AFLFGESMGGLVTLLMYFQSEPETWTGLMFSAPLFVIPEDMKPSKAHLFAYGLLFGLADT 202

Query: 194 LRLVLSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYIKMNCHEIRVP 253
              +       K+ K+  K +++A NP +R++GKP + T  E LR  +Y++ N  ++ +P
Sbjct: 203 WAAMPDNKMVGKAIKDPEKLKIIASNP-QRYTGKPRVGTMRELLRKTQYVQENFGKVTIP 262

Query: 254 MLMVHGEDDVVCDSESARFVFESAASKDKTLKVYPGMWHQLI-GEANESVEIVYTTIFNW 313
           +   HG  D V    S++ ++E A+S DKTLK+Y GM+H LI GE +E+ EIV   +  W
Sbjct: 263 VFTAHGTADGVTCPTSSKLLYEKASSADKTLKIYEGMYHSLIQGEPDENAEIVLKDMREW 322

Query: 314 LVDRAEKAKAK 321
           + ++ +K  +K
Sbjct: 323 IDEKVKKYGSK 330

[Close]

BLAST of Cp4.1LG10g06420.1 vs. ExPASy Swiss-Prot
Match: A0QNZ7 (Monoacylglycerol lipase OS=Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) OX=246196 GN=MSMEG_0220 PE=1 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 7.1e-24
Identity = 75/278 (26.98%), Postives = 124/278 (44.60%), Query Frame = 0


Query: 41  MKIFTQSWRPVSDSELKGVVAMVHGYTSDSGWMFELTAVAIAKAGFLVCSLDLQGHGRSD 100
           ++I    W P  D++ +GVV + HGY   +G  +   A     AG LV +LD +GHGRS 
Sbjct: 17  VRIVYDVWTP--DTDPRGVVVLAHGYAEHAG-RYHHVAQRFGAAGLLVYALDHRGHGRSG 76

Query: 101 GAPGSIPDIELLVLDCTLFFDSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVL 160
           G    + D+   V D           +PTLP  + G S+GG I      +  G +S +VL
Sbjct: 77  GKRVHLRDLSEFVEDFRTLVGIAANDHPTLPRIVLGHSMGGGIVFAYGARYPGEYSAMVL 136

Query: 161 NGSMCGISAKFKPIWPLEKLLPIAASLAPSLRL-------VLSKPHASKSYKEEWKRRLV 220
           +G          P+  L  +  +   LAP + +       V   P    +YK +      
Sbjct: 137 SGPAVNAHDGVSPV--LVAVAKVLGKLAPGIPVENLDADAVSRDPEVVAAYKAD------ 196

Query: 221 AKNPNRRFSGKPPMATALEFLRVCEYIKMNCHEIRVPMLMVHGEDDVVCDSESARFVFES 280
                    GK P   A   + + + +      +  P+L+VHG+ D +     +R + + 
Sbjct: 197 ----PMVHHGKLPAGIARALIGLGQSMPQRAAALTAPLLVVHGDKDRLIPVAGSRLLVDR 256

Query: 281 AASKDKTLKVYPGMWHQLIGEANESVEIVYTTIFNWLV 312
            AS+D  LKVYPG++H++  E  +  ++V   + +W+V
Sbjct: 257 VASEDVHLKVYPGLYHEVFNEPEQ--KLVLDDVTSWIV 277

[Close]

BLAST of Cp4.1LG10g06420.1 vs. ExPASy Swiss-Prot
Match: Q99685 (Monoglyceride lipase OS=Homo sapiens OX=9606 GN=MGLL PE=1 SV=2)

HSP 1 Score: 108.6 bits (270), Expect = 1.3e-22
Identity = 83/289 (28.72%), Postives = 134/289 (46.37%), Query Frame = 0


Query: 35  ILNAQEMKIFTQSWRPVSDSELKGVVAMVHGYTSDSGWMFELTAVAIAKAGFLVCSLDLQ 94
           ++NA    +F + W+P      K ++ + HG    SG  +E  A  +     LV + D  
Sbjct: 22  LVNADGQYLFCRYWKPTGTP--KALIFVSHGAGEHSG-RYEELARMLMGLDLLVFAHDHV 81

Query: 95  GHGRSDGAPGSIPDIELLVLDCTLFFDSVREQNPTLPAFLYGESLGGAISILICLKQEGV 154
           GHG+S+G    + D  + V D     DS+++  P LP FL G S+GGAI+IL   ++ G 
Sbjct: 82  GHGQSEGERMVVSDFHVFVRDVLQHVDSMQKDYPGLPVFLLGHSMGGAIAILTAAERPGH 141

Query: 155 WSGIVLNGSM------CGISAKFKPIWPLEKLLPIAASLAPSLRLVLSKPHASKSYKEEW 214
           ++G+VL   +         + K      L  +LP   SL P    VLS        + + 
Sbjct: 142 FAGMVLISPLVLANPESATTFKVLAAKVLNLVLP-NLSLGPIDSSVLS--------RNKT 201

Query: 215 KRRLVAKNPNRRFSGKPPMATALEFLRVCEYIKMNCHEIRVPMLMVHGEDDVVCDSESAR 274
           +  +   +P    +G   +   ++ L     ++    ++ VP L++ G  D +CDS+ A 
Sbjct: 202 EVDIYNSDPLICRAGL-KVCFGIQLLNAVSRVERALPKLTVPFLLLQGSADRLCDSKGAY 261

Query: 275 FVFESAASKDKTLKVYPGMWHQLIGEANESVEIVYTTIFNWLVDRAEKA 318
            + E A S+DKTLK+Y G +H L  E  E    V+  I  W+  R   A
Sbjct: 262 LLMELAKSQDKTLKIYEGAYHVLHKELPEVTNSVFHEINMWVSQRTATA 297

[Close]

BLAST of Cp4.1LG10g06420.1 vs. ExPASy Swiss-Prot
Match: O35678 (Monoglyceride lipase OS=Mus musculus OX=10090 GN=Mgll PE=1 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 2.5e-21
Identity = 83/295 (28.14%), Postives = 137/295 (46.44%), Query Frame = 0


Query: 35  ILNAQEMKIFTQSWRPVSDSELKGVVAMVHGYTSDSGWMFELTAVAIAKAGFLVCSLDLQ 94
           ++NA    +F + W+P      K ++ + HG     G   EL A  +     LV + D  
Sbjct: 22  LVNADGQYLFCRYWKP--SGTPKALIFVSHGAGEHCGRYDEL-AHMLKGLDMLVFAHDHV 81

Query: 95  GHGRSDGAPGSIPDIELLVLDCTLFFDSVREQNPTLPAFLYGESLGGAISILICLKQEGV 154
           GHG+S+G    + D ++ V D     D++++  P +P FL G S+GGAISIL+  ++   
Sbjct: 82  GHGQSEGERMVVSDFQVFVRDVLQHVDTIQKDYPDVPIFLLGHSMGGAISILVAAERPTY 141

Query: 155 WSGIVLNGSMCGISAKFKPIWPLEKLLPIAAS----LAPSL------RLVLSKPHASKSY 214
           +SG+VL             I PL    P +AS    LA  L       + L +  +S   
Sbjct: 142 FSGMVL-------------ISPLVLANPESASTLKVLAAKLLNFVLPNMTLGRIDSSVLS 201

Query: 215 KEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYIKMNCHEIRVPMLMVHGEDDVVCDS 274
           + + +  L   +P    +G   +   ++ L     ++     + +P L++ G  D +CDS
Sbjct: 202 RNKSEVDLYNSDPLVCRAGL-KVCFGIQLLNAVARVERAMPRLTLPFLLLQGSADRLCDS 261

Query: 275 ESARFVFESAASKDKTLKVYPGMWHQLIGEANESVEIVYTTIFNWLVDRAEKAKA 320
           + A  + ES+ S+DKTLK+Y G +H L  E  E    V   + +W+  R   A A
Sbjct: 262 KGAYLLMESSRSQDKTLKMYEGAYHVLHRELPEVTNSVLHEVNSWVSHRIAAAGA 299

[Close]

BLAST of Cp4.1LG10g06420.1 vs. ExPASy Swiss-Prot
Match: Q8R431 (Monoglyceride lipase OS=Rattus norvegicus OX=10116 GN=Mgll PE=1 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 5.7e-21
Identity = 78/288 (27.08%), Postives = 136/288 (47.22%), Query Frame = 0


Query: 35  ILNAQEMKIFTQSWRPVSDSELKGVVAMVHGYTSDSGWMFELTAVAIAKAGFLVCSLDLQ 94
           ++NA    +F + W+P      K ++ + HG     G   EL A  + +   LV + D  
Sbjct: 22  LVNADGQYLFCRYWKP--SGTPKALIFVSHGAGEHCGRYDEL-AQMLKRLDMLVFAHDHV 81

Query: 95  GHGRSDGAPGSIPDIELLVLDCTLFFDSVREQNPTLPAFLYGESLGGAISILICLKQEGV 154
           GHG+S+G    + D ++ V D     ++V++  P +P FL G S+GGAISIL   ++   
Sbjct: 82  GHGQSEGERMVVSDFQVFVRDLLQHVNTVQKDYPEVPVFLLGHSMGGAISILAAAERPTH 141

Query: 155 WSGIVLNGSMCGISAKFKPIWPLEKLLPIAASLAPSL--RLVLSKPHASKSYKEEWKRRL 214
           +SG++L   +   + +         L  +AA L   +   + L +  +S   + + +  L
Sbjct: 142 FSGMILISPLILANPE-----SASTLKVLAAKLLNFVLPNISLGRIDSSVLSRNKSEVDL 201

Query: 215 VAKNPNRRFSGKPPMATALEFLRVCEYIKMNCHEIRVPMLMVHGEDDVVCDSESARFVFE 274
              +P    +G   +   ++ L     ++     + +P L++ G  D +CDS+ A  + E
Sbjct: 202 YNSDPLICHAG-VKVCFGIQLLNAVSRVERAMPRLTLPFLLLQGSADRLCDSKGAYLLME 261

Query: 275 SAASKDKTLKVYPGMWHQLIGEANESVEIVYTTIFNWLVDRAEKAKAK 321
           S+ S+DKTLK+Y G +H L  E  E    V   I  W+  R   A A+
Sbjct: 262 SSPSQDKTLKMYEGAYHVLHKELPEVTNSVLHEINTWVSHRIAVAGAR 300

[Close]

BLAST of Cp4.1LG10g06420.1 vs. NCBI nr
Match: XP_023543932.1 (caffeoylshikimate esterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 667 bits (1722), Expect = 4.01e-242
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 0


Query: 1   MAHPINQANENSPYGGLTREQFYKKHNVTHHEAFILNAQEMKIFTQSWRPVSDSELKGVV 60
           MAHPINQANENSPYGGLTREQFYKKHNVTHHEAFILNAQEMKIFTQSWRPVSDSELKGVV
Sbjct: 1   MAHPINQANENSPYGGLTREQFYKKHNVTHHEAFILNAQEMKIFTQSWRPVSDSELKGVV 60

Query: 61  AMVHGYTSDSGWMFELTAVAIAKAGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120
           AMVHGYTSDSGWMFELTAVAIAKAGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF
Sbjct: 61  AMVHGYTSDSGWMFELTAVAIAKAGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120

Query: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFKPIWPLEKL 180
           DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFKPIWPLEKL
Sbjct: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFKPIWPLEKL 180

Query: 181 LPIAASLAPSLRLVLSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAPSLRLVLSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI
Sbjct: 181 LPIAASLAPSLRLVLSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI 240

Query: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASKDKTLKVYPGMWHQLIGEANESVE 300
           KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASKDKTLKVYPGMWHQLIGEANESVE
Sbjct: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASKDKTLKVYPGMWHQLIGEANESVE 300

Query: 301 IVYTTIFNWLVDRAEKAKAKNKNKNTKK 328
           IVYTTIFNWLVDRAEKAKAKNKNKNTKK
Sbjct: 301 IVYTTIFNWLVDRAEKAKAKNKNKNTKK 328

[Close]

BLAST of Cp4.1LG10g06420.1 vs. NCBI nr
Match: XP_022925667.1 (caffeoylshikimate esterase-like [Cucurbita moschata])

HSP 1 Score: 649 bits (1674), Expect = 8.34e-235
Identity = 316/328 (96.34%), Postives = 324/328 (98.78%), Query Frame = 0


Query: 1   MAHPINQANENSPYGGLTREQFYKKHNVTHHEAFILNAQEMKIFTQSWRPVSDSELKGVV 60
           MAHPINQANENSPYGGLTRE+FYKKHNVTHHE FILNAQ+MKIFTQSWRP SDSELKGVV
Sbjct: 1   MAHPINQANENSPYGGLTREEFYKKHNVTHHETFILNAQKMKIFTQSWRPASDSELKGVV 60

Query: 61  AMVHGYTSDSGWMFELTAVAIAKAGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120
           AMVHGYTSDSGWMFELTAVAIAK+GFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF
Sbjct: 61  AMVHGYTSDSGWMFELTAVAIAKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120

Query: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFKPIWPLEKL 180
           DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKF+PIWPLEKL
Sbjct: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKL 180

Query: 181 LPIAASLAPSLRLVLSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAP LRLV+SKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI
Sbjct: 181 LPIAASLAPWLRLVVSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI 240

Query: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASKDKTLKVYPGMWHQLIGEANESVE 300
           KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAAS+DKTLKVYPGMWHQLIGEANE+VE
Sbjct: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWHQLIGEANENVE 300

Query: 301 IVYTTIFNWLVDRAEKAKAKNKNKNTKK 328
           IVY TIFNWLVDRAEKAKAKNK+KNTKK
Sbjct: 301 IVYATIFNWLVDRAEKAKAKNKSKNTKK 328

[Close]

BLAST of Cp4.1LG10g06420.1 vs. NCBI nr
Match: KAG6581477.1 (Caffeoylshikimate esterase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034768.1 Caffeoylshikimate esterase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 646 bits (1667), Expect = 9.73e-234
Identity = 315/328 (96.04%), Postives = 324/328 (98.78%), Query Frame = 0


Query: 1   MAHPINQANENSPYGGLTREQFYKKHNVTHHEAFILNAQEMKIFTQSWRPVSDSELKGVV 60
           MAHPINQANENSPYGGLTRE+FYKKHNVTH E FILNAQ+MKIFTQSWRPVSDSELKGVV
Sbjct: 1   MAHPINQANENSPYGGLTREEFYKKHNVTHQETFILNAQKMKIFTQSWRPVSDSELKGVV 60

Query: 61  AMVHGYTSDSGWMFELTAVAIAKAGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120
           AMVHGYTSDSGWMFELTAVAIAK+GFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF
Sbjct: 61  AMVHGYTSDSGWMFELTAVAIAKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120

Query: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFKPIWPLEKL 180
           DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKF+PIWPLEKL
Sbjct: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKL 180

Query: 181 LPIAASLAPSLRLVLSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAP LRLV+SKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI
Sbjct: 181 LPIAASLAPWLRLVVSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI 240

Query: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASKDKTLKVYPGMWHQLIGEANESVE 300
           KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAAS+DKTLKVYPGMWHQLIGEANE+VE
Sbjct: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWHQLIGEANENVE 300

Query: 301 IVYTTIFNWLVDRAEKAKAKNKNKNTKK 328
           IVY TIFNWLVDRAEKAKAKN++KNTKK
Sbjct: 301 IVYATIFNWLVDRAEKAKAKNRSKNTKK 328

[Close]

BLAST of Cp4.1LG10g06420.1 vs. NCBI nr
Match: XP_022977253.1 (caffeoylshikimate esterase-like [Cucurbita maxima])

HSP 1 Score: 635 bits (1638), Expect = 2.38e-229
Identity = 311/328 (94.82%), Postives = 321/328 (97.87%), Query Frame = 0


Query: 1   MAHPINQANENSPYGGLTREQFYKKHNVTHHEAFILNAQEMKIFTQSWRPVSDSELKGVV 60
           MAHPINQANENSPYGGLTRE FY+KHNVTH E FILNAQ+MKIFTQSWRPVSDSELKGVV
Sbjct: 1   MAHPINQANENSPYGGLTREDFYQKHNVTHQETFILNAQKMKIFTQSWRPVSDSELKGVV 60

Query: 61  AMVHGYTSDSGWMFELTAVAIAKAGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120
           AMVHGYTSDSGW+FELTAVA+AK+GFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF
Sbjct: 61  AMVHGYTSDSGWIFELTAVALAKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120

Query: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFKPIWPLEKL 180
           DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKF+PIWPLEKL
Sbjct: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKL 180

Query: 181 LPIAASLAPSLRLVLSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAPSLRLV SKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVC+YI
Sbjct: 181 LPIAASLAPSLRLVASKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCKYI 240

Query: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASKDKTLKVYPGMWHQLIGEANESVE 300
           KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAAS+DKTLKVYPGMWHQLIGEANE+VE
Sbjct: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWHQLIGEANENVE 300

Query: 301 IVYTTIFNWLVDRAEKAKAKNKNKNTKK 328
           IVY TIFNWLVDRAEKAK  NKNK+TKK
Sbjct: 301 IVYATIFNWLVDRAEKAK--NKNKDTKK 326

[Close]

BLAST of Cp4.1LG10g06420.1 vs. NCBI nr
Match: XP_038881607.1 (caffeoylshikimate esterase-like [Benincasa hispida])

HSP 1 Score: 596 bits (1537), Expect = 5.67e-214
Identity = 289/316 (91.46%), Postives = 301/316 (95.25%), Query Frame = 0


Query: 1   MAHPINQANENSPYGGLTREQFYKKHNVTHHEAFILNAQEMKIFTQSWRPVSDSELKGVV 60
           M HPINQANENSPYGGLTRE+FYKKH VTHHE+FILNAQ+MKIFTQSWRP SDSELKGVV
Sbjct: 1   MLHPINQANENSPYGGLTREEFYKKHKVTHHESFILNAQKMKIFTQSWRPDSDSELKGVV 60

Query: 61  AMVHGYTSDSGWMFELTAVAIAKAGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120
           AMVHGYTSDSGW+FELTAVAIAK GFLVCSLDLQGHGRS+GAPGSIPDIELLVLDCTLFF
Sbjct: 61  AMVHGYTSDSGWIFELTAVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTLFF 120

Query: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFKPIWPLEKL 180
           DSVREQNP LPAFLYGESLGGAISILI LKQ+GVW+GIVLNGSMCGISAKFKPIWPLEKL
Sbjct: 121 DSVREQNPNLPAFLYGESLGGAISILISLKQKGVWNGIVLNGSMCGISAKFKPIWPLEKL 180

Query: 181 LPIAASLAPSLRLVLSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAPSLRLV++KP ASKSYKEEWKRRLVA+NPNRRFSGKPPMATALEFLRVCEYI
Sbjct: 181 LPIAASLAPSLRLVITKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240

Query: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASKDKTLKVYPGMWHQLIGEANESVE 300
           K NCHEIRVPMLMVHGEDDVVCDS SARFVFESA S+DKTLKVYPGMWHQLIGE  E VE
Sbjct: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARFVFESAESEDKTLKVYPGMWHQLIGEPKEDVE 300

Query: 301 IVYTTIFNWLVDRAEK 316
           +VY TIF WLVDRAEK
Sbjct: 301 VVYGTIFTWLVDRAEK 316

[Close]

BLAST of Cp4.1LG10g06420.1 vs. ExPASy TrEMBL
Match: A0A6J1ECA8 (caffeoylshikimate esterase-like OS=Cucurbita moschata OX=3662 GN=LOC111433016 PE=4 SV=1)

HSP 1 Score: 649 bits (1674), Expect = 4.04e-235
Identity = 316/328 (96.34%), Postives = 324/328 (98.78%), Query Frame = 0


Query: 1   MAHPINQANENSPYGGLTREQFYKKHNVTHHEAFILNAQEMKIFTQSWRPVSDSELKGVV 60
           MAHPINQANENSPYGGLTRE+FYKKHNVTHHE FILNAQ+MKIFTQSWRP SDSELKGVV
Sbjct: 1   MAHPINQANENSPYGGLTREEFYKKHNVTHHETFILNAQKMKIFTQSWRPASDSELKGVV 60

Query: 61  AMVHGYTSDSGWMFELTAVAIAKAGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120
           AMVHGYTSDSGWMFELTAVAIAK+GFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF
Sbjct: 61  AMVHGYTSDSGWMFELTAVAIAKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120

Query: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFKPIWPLEKL 180
           DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKF+PIWPLEKL
Sbjct: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKL 180

Query: 181 LPIAASLAPSLRLVLSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAP LRLV+SKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI
Sbjct: 181 LPIAASLAPWLRLVVSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI 240

Query: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASKDKTLKVYPGMWHQLIGEANESVE 300
           KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAAS+DKTLKVYPGMWHQLIGEANE+VE
Sbjct: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWHQLIGEANENVE 300

Query: 301 IVYTTIFNWLVDRAEKAKAKNKNKNTKK 328
           IVY TIFNWLVDRAEKAKAKNK+KNTKK
Sbjct: 301 IVYATIFNWLVDRAEKAKAKNKSKNTKK 328

[Close]

BLAST of Cp4.1LG10g06420.1 vs. ExPASy TrEMBL
Match: A0A6J1IPF3 (caffeoylshikimate esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111477621 PE=4 SV=1)

HSP 1 Score: 635 bits (1638), Expect = 1.15e-229
Identity = 311/328 (94.82%), Postives = 321/328 (97.87%), Query Frame = 0


Query: 1   MAHPINQANENSPYGGLTREQFYKKHNVTHHEAFILNAQEMKIFTQSWRPVSDSELKGVV 60
           MAHPINQANENSPYGGLTRE FY+KHNVTH E FILNAQ+MKIFTQSWRPVSDSELKGVV
Sbjct: 1   MAHPINQANENSPYGGLTREDFYQKHNVTHQETFILNAQKMKIFTQSWRPVSDSELKGVV 60

Query: 61  AMVHGYTSDSGWMFELTAVAIAKAGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120
           AMVHGYTSDSGW+FELTAVA+AK+GFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF
Sbjct: 61  AMVHGYTSDSGWIFELTAVALAKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120

Query: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFKPIWPLEKL 180
           DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKF+PIWPLEKL
Sbjct: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKL 180

Query: 181 LPIAASLAPSLRLVLSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAPSLRLV SKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVC+YI
Sbjct: 181 LPIAASLAPSLRLVASKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCKYI 240

Query: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASKDKTLKVYPGMWHQLIGEANESVE 300
           KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAAS+DKTLKVYPGMWHQLIGEANE+VE
Sbjct: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWHQLIGEANENVE 300

Query: 301 IVYTTIFNWLVDRAEKAKAKNKNKNTKK 328
           IVY TIFNWLVDRAEKAK  NKNK+TKK
Sbjct: 301 IVYATIFNWLVDRAEKAK--NKNKDTKK 326

[Close]

BLAST of Cp4.1LG10g06420.1 vs. ExPASy TrEMBL
Match: A0A5A7UE91 (Caffeoylshikimate esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G009620 PE=4 SV=1)

HSP 1 Score: 588 bits (1515), Expect = 7.44e-211
Identity = 284/323 (87.93%), Postives = 302/323 (93.50%), Query Frame = 0


Query: 1   MAHPINQANENSPYGGLTREQFYKKHNVTHHEAFILNAQEMKIFTQSWRPVSDSELKGVV 60
           M+HPINQANENSPYG LTRE+FYKKHNVTHHE+FILNAQ+MKIFTQSWRP SD++LKGVV
Sbjct: 1   MSHPINQANENSPYGELTREEFYKKHNVTHHESFILNAQKMKIFTQSWRPDSDTKLKGVV 60

Query: 61  AMVHGYTSDSGWMFELTAVAIAKAGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120
           AMVHGYT DSGW+FELT VAIAK GFLVCSLDLQGHGRS+GAPGSIPDIELLV DCTLFF
Sbjct: 61  AMVHGYTCDSGWIFELTGVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVHDCTLFF 120

Query: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFKPIWPLEKL 180
           DS+REQ+P LPAFLYGESLGGAISILI LKQEGVW+GIVLNGSMCGIS KFKPIWPLEKL
Sbjct: 121 DSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNGSMCGISGKFKPIWPLEKL 180

Query: 181 LPIAASLAPSLRLVLSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAPSLRLV+SKP ASKSYKEEWKRRLVA+NPNRRFSGKPPMATALEFLRVCEYI
Sbjct: 181 LPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240

Query: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASKDKTLKVYPGMWHQLIGEANESVE 300
           K NCHEIRVPMLMVHGEDDVVCDS SAR+VFE+A S+DKTLKVYPGMWHQLIGEA E VE
Sbjct: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARYVFEAAESEDKTLKVYPGMWHQLIGEAKEDVE 300

Query: 301 IVYTTIFNWLVDRAEKAKAKNKN 323
           IVY TI NWLVD+A+KA   N N
Sbjct: 301 IVYGTILNWLVDKADKANNSNNN 323

[Close]

BLAST of Cp4.1LG10g06420.1 vs. ExPASy TrEMBL
Match: A0A1S3B390 (caffeoylshikimate esterase-like OS=Cucumis melo OX=3656 GN=LOC103485302 PE=4 SV=1)

HSP 1 Score: 585 bits (1509), Expect = 6.11e-210
Identity = 283/323 (87.62%), Postives = 301/323 (93.19%), Query Frame = 0


Query: 1   MAHPINQANENSPYGGLTREQFYKKHNVTHHEAFILNAQEMKIFTQSWRPVSDSELKGVV 60
           M+HPINQANENSPYG LTRE+FYKKHNVTHHE+FILNAQ+MKIFTQSWRP SD++LKGVV
Sbjct: 1   MSHPINQANENSPYGELTREEFYKKHNVTHHESFILNAQKMKIFTQSWRPDSDTKLKGVV 60

Query: 61  AMVHGYTSDSGWMFELTAVAIAKAGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120
           AMVHGYT DSGW+FELT VAIAK GFLVCSLDLQGHGRS+GAPGSIPDIELLV DCTLFF
Sbjct: 61  AMVHGYTCDSGWIFELTGVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVHDCTLFF 120

Query: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFKPIWPLEKL 180
           DS+REQ+P LPAFLYGESLGGAISILI LKQEGVW+GIVLNG MCGIS KFKPIWPLEKL
Sbjct: 121 DSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNGFMCGISGKFKPIWPLEKL 180

Query: 181 LPIAASLAPSLRLVLSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAPSLRLV+SKP ASKSYKEEWKRRLVA+NPNRRFSGKPPMATALEFLRVCEYI
Sbjct: 181 LPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240

Query: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASKDKTLKVYPGMWHQLIGEANESVE 300
           K NCHEIRVPMLMVHGEDDVVCDS SAR+VFE+A S+DKTLKVYPGMWHQLIGEA E VE
Sbjct: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARYVFEAAESEDKTLKVYPGMWHQLIGEAKEDVE 300

Query: 301 IVYTTIFNWLVDRAEKAKAKNKN 323
           IVY TI NWLVD+A+KA   N N
Sbjct: 301 IVYGTILNWLVDKADKANNSNNN 323

[Close]

BLAST of Cp4.1LG10g06420.1 vs. ExPASy TrEMBL
Match: A0A0A0KKV9 (Hydrolase_4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G525500 PE=4 SV=1)

HSP 1 Score: 582 bits (1500), Expect = 1.33e-208
Identity = 279/317 (88.01%), Postives = 301/317 (94.95%), Query Frame = 0


Query: 1   MAHPINQANENSPYGGLTREQFYKKHNVTHHEAFILNAQEMKIFTQSWRPVSDSELKGVV 60
           M+HPI+QANENSPYG LTRE+FYKKHNVTHHE+FILNAQ+MKIFTQSW+P SDS+LKGVV
Sbjct: 1   MSHPISQANENSPYGELTREEFYKKHNVTHHESFILNAQKMKIFTQSWQPDSDSKLKGVV 60

Query: 61  AMVHGYTSDSGWMFELTAVAIAKAGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120
           AMVHGYT DSGW+FELT +AIAK GFLVCSLDLQGHGRS+GAPGSI DIELLVLDCT FF
Sbjct: 61  AMVHGYTCDSGWIFELTGIAIAKLGFLVCSLDLQGHGRSEGAPGSIRDIELLVLDCTQFF 120

Query: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFKPIWPLEKL 180
           DS+REQ+P LPAFLYGESLGGAISILI LKQEGVW+GIVLNGSMCGISAKFKPIWPLEKL
Sbjct: 121 DSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNGSMCGISAKFKPIWPLEKL 180

Query: 181 LPIAASLAPSLRLVLSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAPSLRLV+SKP ASKSYKEEWKRRLVA+NPNRRFSGKPPMATALEFLRVCEYI
Sbjct: 181 LPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240

Query: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASKDKTLKVYPGMWHQLIGEANESVE 300
           K NCHEIRVP+LMVHGEDDVVCDS SAR+V+E+A S+DKTLKVYPGMWHQLIGE  E+VE
Sbjct: 241 KRNCHEIRVPLLMVHGEDDVVCDSWSARYVYEAAESEDKTLKVYPGMWHQLIGETKENVE 300

Query: 301 IVYTTIFNWLVDRAEKA 317
           +VY TIFNWLVDRAEKA
Sbjct: 301 VVYGTIFNWLVDRAEKA 317

[Close]

BLAST of Cp4.1LG10g06420.1 vs. TAIR 10
Match: AT5G14980.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 336.7 bits (862), Expect = 2.2e-92
Identity = 155/316 (49.05%), Postives = 227/316 (71.84%), Query Frame = 0


Query: 3   HPINQANENSPYGGLTREQFYKKHNVTHHEAFILNAQEMKIFTQSWRPVSDSELKGVVAM 62
           HP+++AN +SP+G L+  +FY +H+VTH  A++ N   +K+FTQ W P++   L G++A+
Sbjct: 4   HPVSEANASSPFGSLSAAEFYSRHSVTHSSAYVTNPTGLKLFTQWWTPLNRPPL-GLIAV 63

Query: 63  VHGYTSDSGWMFELTAVAIAKAGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFFDS 122
           VHG+T +S W  +LT+V  AK+G+L C++D QGHG SDG    IP+I L+V DC  FFD 
Sbjct: 64  VHGFTGESSWFLQLTSVLFAKSGYLTCAIDHQGHGFSDGLTAHIPNINLIVDDCISFFDD 123

Query: 123 VREQNPT--LPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFKPIWPLEKL 182
            R+++ +  LPAFLY ESLGGAI++ I L+Q+  W+G++L+G+MC IS KFKP WPL+ L
Sbjct: 124 FRKRHASSFLPAFLYSESLGGAIALYITLRQKHQWNGLILSGAMCSISHKFKPPWPLQHL 183

Query: 183 LPIAASLAPSLRLVLSKPH-ASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEY 242
           L +AA+L P+ R+V ++   A  S+KE WKR+L   NPNR   GKP  ATA E +RVCE 
Sbjct: 184 LTLAATLIPTWRVVPTRGSIAGVSFKEPWKRKLAYANPNRTV-GKPRAATAYELVRVCED 243

Query: 243 IKMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASKDKTLKVYPGMWHQLIGEANESV 302
           ++    E+ VP+++VHG DDVVCD  S   ++   +S+DKT+K+YPGMWHQLIGE+ E+V
Sbjct: 244 LQNRFEEVEVPLMIVHGRDDVVCDPASVEELYRRCSSRDKTIKIYPGMWHQLIGESEENV 303

Query: 303 EIVYTTIFNWLVDRAE 316
           ++V+  + +W+  R+E
Sbjct: 304 DLVFGDVLDWIKTRSE 317

[Close]

BLAST of Cp4.1LG10g06420.1 vs. TAIR 10
Match: AT5G19290.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 331.6 bits (849), Expect = 7.0e-91
Identity = 160/321 (49.84%), Postives = 223/321 (69.47%), Query Frame = 0


Query: 3   HPINQANENSPYGGLTREQFYKKHNVTHHEAFILNAQEMKIFTQSWRPVSDSELKGVVAM 62
           HPI+ ANE++P+G LT ++FY KH+V+H  AFI N + +K+FTQ W P+  ++  G++A+
Sbjct: 4   HPISDANEHNPFGSLTADEFYAKHSVSHSSAFITNPRGLKLFTQWWSPLPPTKPIGIIAV 63

Query: 63  VHGYTSDSGWMFELTAVAIAKAGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFFDS 122
           VHG+T +S W  +LT++  AK+GF+ C++D QGHG SDG    IPDI  +V DC  FFD 
Sbjct: 64  VHGFTGESSWFLQLTSILFAKSGFITCAIDHQGHGFSDGLIAHIPDINPVVDDCISFFDD 123

Query: 123 VR-EQNPT-LPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFKPIWPLEKL 182
            R  Q P+ LP FLY ESLGGAI++ I L+Q GVW G++LNG+MCGIS KFKP WPLE L
Sbjct: 124 FRSRQTPSDLPCFLYSESLGGAIALYISLRQRGVWDGLILNGAMCGISDKFKPPWPLEHL 183

Query: 183 LPIAASLAPSLRLVLSKPH-ASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEY 242
           L + A+L P+ R++ ++      S+KE WKR+L   +P RR   KP  ATA E +RVC+ 
Sbjct: 184 LFVVANLIPTWRVIPTRGSIPDVSFKEPWKRKLAMASP-RRTVAKPRAATAYELIRVCKD 243

Query: 243 IKMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASKDKTLKVYPGMWHQLIGEANESV 302
           ++    E+ VP+L+VHG  DVVCD      +   A S+DKT+K+YP +WHQ+IGE+ E V
Sbjct: 244 LQGRFEEVEVPLLIVHGGGDVVCDVACVEELHRRAISEDKTIKIYPELWHQMIGESEEKV 303

Query: 303 EIVYTTIFNWLVDRAE-KAKA 320
           ++VY  + +WL  RAE KA+A
Sbjct: 304 DLVYGDMLSWLKSRAERKARA 323

[Close]

BLAST of Cp4.1LG10g06420.1 vs. TAIR 10
Match: AT1G11090.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 233.4 bits (594), Expect = 2.6e-61
Identity = 120/313 (38.34%), Postives = 188/313 (60.06%), Query Frame = 0


Query: 14  YGGLTREQFYKKHNVTHHEAFILNAQEMKIFTQSWRPVSDSELKGVVAMVHGYTSDSGWM 73
           +G  + E ++    +   ++F  + + + +FT+SW P S S  +G++ MVHGY +D  W 
Sbjct: 13  WGETSEEDYFNLKGIIGSKSFFTSPRGLNLFTRSWLPSSSSPPRGLIFMVHGYGNDVSWT 72

Query: 74  FELTAVAIAKAGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFFDSVREQNPT---L 133
           F+ T + +A+ GF   +LD++GHGRSDG    +P ++L+V D   FF+S++ QNP    L
Sbjct: 73  FQSTPIFLAQMGFACFALDIEGHGRSDGVRAYVPSVDLVVDDIISFFNSIK-QNPKFQGL 132

Query: 134 PAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFKPIWPLEKLLPIAASLAPS 193
           P FL+GES+GGAI +LI       + G VL   MC IS K +P WP+++ L + +   P+
Sbjct: 133 PRFLFGESMGGAICLLIQFADPLGFDGAVLVAPMCKISDKVRPKWPVDQFLIMISRFLPT 192

Query: 194 LRLVLSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYIKMNCHEIRVP 253
             +V ++    KS K E K+ +  +NP  R++ KP + T +E LRV +Y+     ++ +P
Sbjct: 193 WAIVPTEDLLEKSIKVEEKKPIAKRNP-MRYNEKPRLGTVMELLRVTDYLGKKLKDVSIP 252

Query: 254 MLMVHGEDDVVCDSESARFVFESAASKDKTLKVYPGMWH-QLIGEANESVEIVYTTIFNW 313
            ++VHG  D V D E +R ++E A SKDKTLK+Y GM H  L GE ++++EIV   I +W
Sbjct: 253 FIIVHGSADAVTDPEVSRELYEHAKSKDKTLKIYDGMMHSMLFGEPDDNIEIVRKDIVSW 312

Query: 314 LVDRAEKAKAKNK 323
           L DR    K K +
Sbjct: 313 LNDRCGGDKTKTQ 323

[Close]

BLAST of Cp4.1LG10g06420.1 vs. TAIR 10
Match: AT2G39400.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 199.5 bits (506), Expect = 4.1e-51
Identity = 108/290 (37.24%), Postives = 166/290 (57.24%), Query Frame = 0


Query: 28  VTHHEAFILNAQEMKIFTQSWRPVSDSELKGVVAMVHGYTSDSGWMFELTAVAIAKAGFL 87
           V + E F+LN++ MK+FT  W+PV   E K ++ + HGY  +S       A  +A AGF 
Sbjct: 2   VMYEEDFVLNSRGMKLFTCVWKPVK-QEPKALLFLCHGYAMESSITMNSAATRLANAGFA 61

Query: 88  VCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFFDSV--REQNPTLPAFLYGESLGGAISI 147
           V  +D +GHG+S+G  G I + + LV D +  + ++  RE+N     FL GES+GGA+ +
Sbjct: 62  VYGMDYEGHGKSEGLNGYISNFDDLVDDVSNHYSTICEREENKGKMRFLLGESMGGAVVL 121

Query: 148 LICLKQEGVWSGIVLNGSMCGISAKFKPIWPLEKLLPIAASLAPSLRLVLSKPHASKSYK 207
           L+  K+   W G VL   MC ++ + KP   +  +L   A   P+ ++V        + K
Sbjct: 122 LLARKKPDFWDGAVLVAPMCKLADEIKPHPVVISILIKLAKFIPTWKIVPGNDIIDIAIK 181

Query: 208 EEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYIKMNCHEIRVPMLMVHGEDDVVCDSE 267
           E   R  V +N    + G+P + TA + L V   ++ N H++ +P +++HGEDD V D  
Sbjct: 182 EPHIRNQVREN-KYCYKGRPRLNTAYQLLLVSLDLEKNLHQVSIPFIVLHGEDDKVTDKS 241

Query: 268 SARFVFESAASKDKTLKVYPGMWHQLI-GEANESVEIVYTTIFNWLVDRA 315
            ++ ++E A+S DKT K+YP MWH L+ GE NE+ EIV+  I NWL DRA
Sbjct: 242 ISKMLYEVASSSDKTFKLYPKMWHALLYGETNENSEIVFGDIINWLEDRA 289

[Close]

BLAST of Cp4.1LG10g06420.1 vs. TAIR 10
Match: AT2G39420.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 196.8 bits (499), Expect = 2.7e-50
Identity = 109/306 (35.62%), Postives = 174/306 (56.86%), Query Frame = 0


Query: 27  NVTHHEAFILNAQEMKIFTQSWRPVSDSELKGVVAMVHGYTSDSGWMFELTAVAIAKAGF 86
           N+ + E+FI N + MK+FT  W P +  E K +V + HGY  +       TA  + KAGF
Sbjct: 7   NIKYEESFIKNTRGMKLFTCKWVP-AKQEPKALVFICHGYAMECSITMNSTARRLVKAGF 66

Query: 87  LVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFFDSV--REQNPTLPAFLYGESLGGAIS 146
            V  +D +GHG+SDG    +P+ + LV D +  + S+  +E+N     FL GES+GGA+ 
Sbjct: 67  AVYGIDYEGHGKSDGLSAYVPNFDHLVDDVSTHYTSICEKEENKGKMRFLLGESMGGAVL 126

Query: 147 ILICLKQEGVWSGIVLNGSMCGISAKFKPIWPLEKLLPIAASLAPSLRLVLSKPHASKSY 206
           +L+  K+   W G VL   MC I+ + KP   +  +L   + + PS +++  +     ++
Sbjct: 127 LLLHRKKPQFWDGAVLVAPMCKIAEEMKPSPLVISILAKLSGVIPSWKIIPGQDIIETAF 186

Query: 207 KEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYIKMNCHEIRVPMLMVHGEDDVVCDS 266
           K+   R+ V +NP   + G+P + TA E LRV   ++   +E+ +P +++HGEDD V D 
Sbjct: 187 KQPEIRKQVRENP-YCYKGRPRLKTAYELLRVSTDLEKRLNEVSLPFIVLHGEDDKVTDK 246

Query: 267 ESARFVFESAASKDKTLKVYPGMWHQLI-GEANESVEIVYTTIFNWL----VDRAEKAKA 326
             +R ++E A+S DKT K+YPGMWH L+ GE  E++E V+  I  WL     D +   ++
Sbjct: 247 AVSRQLYEVASSSDKTFKLYPGMWHGLLYGETPENIETVFADIIGWLDKKVADESGGFES 306

The following BLAST results are available for this feature:

BLAST of Cp4.1LG10g06420.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C942	7.8e-47	34.08	Caffeoylshikimate esterase OS=Arabidopsis thaliana OX=3702 GN=CSE PE=1 SV=1	[more]
A0QNZ7	7.1e-24	26.98	Monoacylglycerol lipase OS=Mycolicibacterium smegmatis (strain ATCC 700084 / mc(...	[more]
Q99685	1.3e-22	28.72	Monoglyceride lipase OS=Homo sapiens OX=9606 GN=MGLL PE=1 SV=2	[more]
O35678	2.5e-21	28.14	Monoglyceride lipase OS=Mus musculus OX=10090 GN=Mgll PE=1 SV=1	[more]
Q8R431	5.7e-21	27.08	Monoglyceride lipase OS=Rattus norvegicus OX=10116 GN=Mgll PE=1 SV=1	[more]

BLAST of Cp4.1LG10g06420.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023543932.1	4.01e-242	100.00	caffeoylshikimate esterase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022925667.1	8.34e-235	96.34	caffeoylshikimate esterase-like [Cucurbita moschata]	[more]
KAG6581477.1	9.73e-234	96.04	Caffeoylshikimate esterase, partial [Cucurbita argyrosperma subsp. sororia] >KAG...	[more]
XP_022977253.1	2.38e-229	94.82	caffeoylshikimate esterase-like [Cucurbita maxima]	[more]
XP_038881607.1	5.67e-214	91.46	caffeoylshikimate esterase-like [Benincasa hispida]	[more]

BLAST of Cp4.1LG10g06420.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ECA8	4.04e-235	96.34	caffeoylshikimate esterase-like OS=Cucurbita moschata OX=3662 GN=LOC111433016 PE...	[more]
A0A6J1IPF3	1.15e-229	94.82	caffeoylshikimate esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111477621 PE=4...	[more]
A0A5A7UE91	7.44e-211	87.93	Caffeoylshikimate esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A1S3B390	6.11e-210	87.62	caffeoylshikimate esterase-like OS=Cucumis melo OX=3656 GN=LOC103485302 PE=4 SV=...	[more]
A0A0A0KKV9	1.33e-208	88.01	Hydrolase_4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G525500...	[more]

BLAST of Cp4.1LG10g06420.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G14980.1	2.2e-92	49.05	alpha/beta-Hydrolases superfamily protein	[more]
AT5G19290.1	7.0e-91	49.84	alpha/beta-Hydrolases superfamily protein	[more]
AT1G11090.1	2.6e-61	38.34	alpha/beta-Hydrolases superfamily protein	[more]
AT2G39400.1	4.1e-51	37.24	alpha/beta-Hydrolases superfamily protein	[more]
AT2G39420.1	2.7e-50	35.62	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000073	Alpha/beta hydrolase fold-1	PRINTS	PR00111	ABHYDROLASE	coord: 251..265 score: 30.8 coord: 85..100 score: 37.07 coord: 134..147 score: 40.64
IPR022742	Serine aminopeptidase, S33	PFAM	PF12146	Hydrolase_4	coord: 55..295 e-value: 4.8E-57 score: 193.0
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 19..323 e-value: 3.6E-53 score: 182.7
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 38..312
None	No IPR available	PANTHER	PTHR11614:SF101	CAFFEOYLSHIKIMATE ESTERASE-LIKE	coord: 2..319
None	No IPR available	PANTHER	PTHR11614	PHOSPHOLIPASE-RELATED	coord: 2..319

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cp4.1LG10g06420	Cp4.1LG10g06420	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG10g06420.1:exon:001	Cp4.1LG10g06420.1:exon:001	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG10g06420.1:cds:001	Cp4.1LG10g06420.1:cds:001	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG10g06420.1	Cp4.1LG10g06420.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0016298	lipase activity