Cp4.1LG07g01320.1 (mRNA) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG07g01320.1
TypemRNA
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionmelanoma-associated antigen 1
LocationCp4.1LG07: 754253 .. 757525 (+)
Sequence length974
RNA-Seq ExpressionCp4.1LG07g01320.1
SyntenyCp4.1LG07g01320.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TAAACAAGAAGGAGAAAAAAAGAAGAAGAAAGCGCGCTCACCGCCCCCAGGTCGAACTGCGCGGGAAGATGCCGAGCTCTTCAAACCTTGGAGGTTTCTGAAGCTTCCCTAAAATTCCGACATCACTTTCGCCGGAAGTCTCAAAGATTTCCCGTCGCTGTTTTCAGCGCTTCAATTCTCCTTTTTATCTTATCGTCGGCTTAGTCCGGGGTAAGCTATGGAATTACTTCAGTTTTATTATCAATAGTCTCTCATGTTTTGTCAATATTGTCTATTAGAATCACTTTTCATCCACGATCAAGCCCGACCTATCTGTTAAAATCGTTCTTGTTGATGTATATATATATATGTTCATATATAGTGATTTTGGAAATCCACCGTTGTTCTGTCATGACTTCAGTTGTTGATTTCGATTGCATATGTTCATTTGACCTCGAATTGATTTTGATTTTCGTTTTATTGTAAGGGAGATTGTTGAGTATGGCAAGTTCAGGAGATTATCTTTCCCAGATTGACATTTCGAACGAGGTATGAGATTGTTCTCTCTGCATAAAGCCCTGTATGAACTATTTGGAAATTGAGGGTTTGCTCCTTGTATCGCCCTCGCATGGTTAATATTTCTGATTCCGAAACCACTTTTAATCGATAGGAAAAGGACAAGCTCGTGGCGGAAGTTATCCGCTATATCATATTCAAAACTCATCAAAATTCTGGATGTCCCATCAAAAGGGAAGAGCTTACTCAGATCGTAACAAAAAATTATAGGAATCGAGGATTGCCGGCTATTGTGATAGAAGAAGCTAAAGAGAAGCTGTCAAGCATTTTTGGTTATGAGTTGAGGGAACTTCAAAGAGCACGGCCTTCATCCACTGCCCAAGGGCACCCTCCTCAACATAGTAAGAAGTGATCTAACTTATTATCTTACTCTTAATTATTACGATCGAGATCTTCAGACGTCGGTCCAACTCCCATTTATTCACGTTATCTTACTGCAATCCTAGTTATCAGAATTTTTGTAGATATGACTACTGCAAGTCACCACAGGTTTCTTTTAACAAACAGTACAGGGTTATGGTTCAGTCTTGTCATGAGTCGAAGCATACAAATTTTAAGAATGAGCTAACCTTTTGCAACTTTAGTATAGAAAAAGTGTCCAACTAATGATCTTGTCAGTGGAACAAAATGTTCTTGTGGGTGACGAGTACATAGGATGAGAAATTCATTTCTTCTAGTAATGTTATTGTCATTACTCGATCCGTGAGCTGTGCACATGCTTTATATTAAATGTGGTTCATTGATCATTTTATTACTTATGCTACACACTCCTATCTCAAGAACCATATATGTGAAATAGCACATATTCGAATTTATTCTCATCTCAGAAACTGCGTACGCCTAGGTTTTGGTTCATTCTTCAAAATAAACGTCTGATGCATCTGATATACAATCCTCTTTATTCATTGCATCGAAATAAAACAACTGGAACATGAGGGTTGGCATTTTCTTAGCATGAAAGTTGTGGCTATCGGTTGTAGAAAGTCATACTAAATTCAGTGGATGTACAGACTGCTGAATGTGGTTATTTGGTTATAAATTTTCTTAGTCGCTGAATAAGCTCTCATTTCGTCAAGAACTAGGATCTGCTGTATGCTTCATGGCTTGCCATTAATATTCTAATTTTCAGGTTACTTCCATCTGACATGGCTTACTGCAAAACTATATCGTTATTGGATCTTGCTTTAACTCTTGTCACTACATGCCTTCAGATGTTGTAGATTCAAAATCCTATGTTCTCATAAGCAAGCTTCCTGCGGAGGTTTATAGAAAGTATGTTGAGGATGTCGATACCGCACACGTGACTGGTTTTAATTTTGTTGTAGTTAGTATTGTACATCTGGCTGGAGGCAAAATTCCTGAAGGTTAGCTCTATGGCTGTAGTTTCTTAATCCAATGCATACTTGGTAGATTTAGAGTGATAGAAAGACAGCTTACACATCAAATGGATTTCATTCATAAACTTTTTCTTTCAATTGTAACTCAAGCCCACCGCTAGCAGATATTGTCCTCTTTGGGCTTTCCGTTTCGAGCTTCTCCTCAAGGTTTTCCCGTTTTAAAAATCTTGAGGGGAAGCCCAAATGGGAAAGCCCAAAGAGGACAATATTTGTTAGCAGTGGGCTTGGGCCGTTACATTACCTGGAGAAGACGTTGCTTATCAGATAGTTATACTAAGTTTTATACCATATTAACTGACTAAAAAATATTTCAATGAGTTTCTTAGAGCAAAAGTTCCAATTTTTTTGCTTCTCTTTCATCGAATCTAGTACATTACTTCGAAAAGATGTTGCTTATCAGATAGCCATGCTACATTTATGCAATATTGATCGACTCAAAATGTTTCAAAGAGTTTCTTAGGGCAGAATGCCATTTTTTGCTTTATAATGGAACCTGGCAATACACTGCAGAAAGCCTTTGGCATCACTTGAGACGAATGGGGTTATCTGAAAGTGATGAAAAACATCCAGTCCTTGGCAACATCAAGCAGGCAGTAGAGTTGCTTGTTCAGCAAAGGTCAGTAAACTCTGTACTATATGCTTGGTAACTGCAATTGAGAAACTTCAACATAATTCGATGACCATAGAAGATACTGGAAAGACGGGTTTACAGGTTCATCTTATTCTTGCCGTGCTTTGTAGATATCTGCAGAAGGACAAAATAAATGGTCCTGAAGGGAATATCACGTTCTACGAGCTTGCTGAAAGAGCTATAGATGGACCAGTTAGTGAAAAGATTAAAGAATATGTAGCACAGGTATGCAGCTTGTCTATATGTATATATAGTATGGATGAATATGTTGTTTTTAGTATAAATAAATGATAATCTTGCAGCATAAAACCTCATAAAGTAGCTAAATTTAAACTTATTATTTGCTTACTTTGGAAGAAGATTAAATGAACACGAGGAATAAAATCCGATAATCGTATTGTCGTGCATTTTAGATTATGTGCCGAAAAATATACAGGATGTCTATCAATAATTAGTTTTGCGTAATTTCTTAAGTAGCATAAAAATTGTGCTTAATATGGATGGTTGGTTGGACGAATCAATGCTACCATGAATGATGGAGCCCATCTTCTGAAGGATTCGCTGTTTATCTGTCTGGAGTGATATCTGTCTGGTTTCTGTTGAATCTAGTTTGCTTTCAGCTTATCATTGCATGTTTCTAATAATTGTGCTTCGACAGCATCAGATCATAGCTTCACGCATTCGACTTAG

mRNA sequence

TAAACAAGAAGGAGAAAAAAAGAAGAAGAAAGCGCGCTCACCGCCCCCAGGTCGAACTGCGCGGGAAGATGCCGAGCTCTTCAAACCTTGGAGGTTTCTGAAGCTTCCCTAAAATTCCGACATCACTTTCGCCGGAAGTCTCAAAGATTTCCCGTCGCTGTTTTCAGCGCTTCAATTCTCCTTTTTATCTTATCGTCGGCTTAGTCCGGGGGAGATTGTTGAGTATGGCAAGTTCAGGAGATTATCTTTCCCAGATTGACATTTCGAACGAGGAAAAGGACAAGCTCGTGGCGGAAGTTATCCGCTATATCATATTCAAAACTCATCAAAATTCTGGATGTCCCATCAAAAGGGAAGAGCTTACTCAGATCGTAACAAAAAATTATAGGAATCGAGGATTGCCGGCTATTGTGATAGAAGAAGCTAAAGAGAAGCTGTCAAGCATTTTTGGTTATGAGTTGAGGGAACTTCAAAGAGCACGGCCTTCATCCACTGCCCAAGGGCACCCTCCTCAACATAATGTTGTAGATTCAAAATCCTATGTTCTCATAAGCAAGCTTCCTGCGGAGGTTTATAGAAAGTATGTTGAGGATGTCGATACCGCACACGTGACTGGTTTTAATTTTGTTGTAGTTAGTATTGTACATCTGGCTGGAGGCAAAATTCCTGAAGAAAGCCTTTGGCATCACTTGAGACGAATGGGGTTATCTGAAAGTGATGAAAAACATCCAGTCCTTGGCAACATCAAGCAGGCAGTAGAGTTGCTTGTTCAGCAAAGATATCTGCAGAAGGACAAAATAAATGGTCCTGAAGGGAATATCACGTTCTACGAGCTTGCTGAAAGAGCTATAGATGGACCAGTTAGTGAAAAGATTAAAGAATATGTAGCACAGTTTGCTTTCAGCTTATCATTGCATGTTTCTAATAATTGTGCTTCGACAGCATCAGATCATAGCTTCACGCATTCGACTTAG

Coding sequence (CDS)

ATGGCAAGTTCAGGAGATTATCTTTCCCAGATTGACATTTCGAACGAGGAAAAGGACAAGCTCGTGGCGGAAGTTATCCGCTATATCATATTCAAAACTCATCAAAATTCTGGATGTCCCATCAAAAGGGAAGAGCTTACTCAGATCGTAACAAAAAATTATAGGAATCGAGGATTGCCGGCTATTGTGATAGAAGAAGCTAAAGAGAAGCTGTCAAGCATTTTTGGTTATGAGTTGAGGGAACTTCAAAGAGCACGGCCTTCATCCACTGCCCAAGGGCACCCTCCTCAACATAATGTTGTAGATTCAAAATCCTATGTTCTCATAAGCAAGCTTCCTGCGGAGGTTTATAGAAAGTATGTTGAGGATGTCGATACCGCACACGTGACTGGTTTTAATTTTGTTGTAGTTAGTATTGTACATCTGGCTGGAGGCAAAATTCCTGAAGAAAGCCTTTGGCATCACTTGAGACGAATGGGGTTATCTGAAAGTGATGAAAAACATCCAGTCCTTGGCAACATCAAGCAGGCAGTAGAGTTGCTTGTTCAGCAAAGATATCTGCAGAAGGACAAAATAAATGGTCCTGAAGGGAATATCACGTTCTACGAGCTTGCTGAAAGAGCTATAGATGGACCAGTTAGTGAAAAGATTAAAGAATATGTAGCACAGTTTGCTTTCAGCTTATCATTGCATGTTTCTAATAATTGTGCTTCGACAGCATCAGATCATAGCTTCACGCATTCGACTTAG

Protein sequence

MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLPAIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKYVEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSESDEKHPVLGNIKQAVELLVQQRYLQKDKINGPEGNITFYELAERAIDGPVSEKIKEYVAQFAFSLSLHVSNNCASTASDHSFTHST
Homology
BLAST of Cp4.1LG07g01320.1 vs. ExPASy Swiss-Prot
Match: P43361 (Melanoma-associated antigen 8 OS=Homo sapiens OX=9606 GN=MAGEA8 PE=1 SV=2)

HSP 1 Score: 66.6 bits (161), Expect = 4.5e-10
Identity = 56/209 (26.79%), Postives = 101/209 (48.33%), Query Frame = 0

Query: 16  EEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLPAIVIEEAKEKLSSIF 75
           E  D+ VAE++R+++ K       P+ + E+ + V KNY+N   P I   +A E +  IF
Sbjct: 110 EALDEKVAELVRFLLRKYQIKE--PVTKAEMLESVIKNYKNH-FPDI-FSKASECMQVIF 169

Query: 76  GYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKYVEDVDTAHVTGFNFV 135
           G +++E+  A       GH          SY+L++ L    Y   + D  +   TG   +
Sbjct: 170 GIDVKEVDPA-------GH----------SYILVTCLGLS-YDGLLGDDQSTPKTGLLII 229

Query: 136 VVSIVHLAGGKIPEESLWHHLRRMGLSESDEKHPVLGNIKQAV-ELLVQQRYLQKDKING 195
           V+ ++ + G + PEE++W  L  MGL +  E H V   +++ + +  VQ+ YL+  +  G
Sbjct: 230 VLGMILMEGSRAPEEAIWEALSVMGLYDGRE-HSVYWKLRKLLTQEWVQENYLEYRQAPG 289

Query: 196 PEGNITFYELAERAIDGPVSEKIKEYVAQ 224
            +     +    RA+      K+ E+V +
Sbjct: 290 SDPVRYEFLWGPRALAETSYVKVLEHVVR 295

BLAST of Cp4.1LG07g01320.1 vs. ExPASy Swiss-Prot
Match: P43363 (Melanoma-associated antigen 10 OS=Homo sapiens OX=9606 GN=MAGEA10 PE=2 SV=2)

HSP 1 Score: 64.3 bits (155), Expect = 2.2e-09
Identity = 48/185 (25.95%), Postives = 91/185 (49.19%), Query Frame = 0

Query: 13  ISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLPAIVIEEAKEKLS 72
           +   E D+ V +++++++FK       PI + E+ + V +NY +     ++  EA E + 
Sbjct: 129 LPRSEIDEKVTDLVQFLLFKYQMKE--PITKAEILESVIRNYEDHF--PLLFSEASECML 188

Query: 73  SIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKYVEDVDTAHVTGF 132
            +FG +++E+            P  H      S+VL++ L    Y   + DV +   TG 
Sbjct: 189 LVFGIDVKEVD-----------PTGH------SFVLVTSL-GLTYDGMLSDVQSMPKTGI 248

Query: 133 NFVVVSIVHLAGGKIPEESLWHHLRRMGLSESDEKHPVLGNIKQAV-ELLVQQRYLQKDK 192
             +++SIV + G   PEE +W  L  MGL +  E H + G  ++ + +  VQ+ YL+  +
Sbjct: 249 LILILSIVFIEGYCTPEEVIWEALNMMGLYDGME-HLIYGEPRKLLTQDWVQENYLEYRQ 290

Query: 193 INGPE 197
           + G +
Sbjct: 309 VPGSD 290

BLAST of Cp4.1LG07g01320.1 vs. ExPASy Swiss-Prot
Match: P43355 (Melanoma-associated antigen 1 OS=Homo sapiens OX=9606 GN=MAGEA1 PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.9e-09
Identity = 58/205 (28.29%), Postives = 100/205 (48.78%), Query Frame = 0

Query: 20  KLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLPAIVIEEAKEKLSSIFGYEL 79
           K VA+++ +++ K       P+ + E+ + V KNY++   P I   +A E L  +FG ++
Sbjct: 104 KKVADLVGFLLLKYRARE--PVTKAEMLESVIKNYKH-CFPEI-FGKASESLQLVFGIDV 163

Query: 80  RELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKYVEDVDTAHVTGFNFVVVSI 139
           +E   A P+    GH          SYVL++ L    Y   + D      TGF  +V+ +
Sbjct: 164 KE---ADPT----GH----------SYVLVTCLGLS-YDGLLGDNQIMPKTGFLIIVLVM 223

Query: 140 VHLAGGKIPEESLWHHLRRMGLSESDEKHPVLGNIKQAV-ELLVQQRYLQKDKINGPEGN 199
           + + GG  PEE +W  L  M + +  E H   G  ++ + + LVQ++YL+  ++  P+ +
Sbjct: 224 IAMEGGHAPEEEIWEELSVMEVYDGRE-HSAYGEPRKLLTQDLVQEKYLEYRQV--PDSD 283

Query: 200 ITFYEL--AERAIDGPVSEKIKEYV 222
              YE     RA+      K+ EYV
Sbjct: 284 PARYEFLWGPRALAETSYVKVLEYV 283

BLAST of Cp4.1LG07g01320.1 vs. ExPASy Swiss-Prot
Match: P43364 (Melanoma-associated antigen 11 OS=Homo sapiens OX=9606 GN=MAGEA11 PE=1 SV=2)

HSP 1 Score: 48.5 bits (114), Expect = 1.3e-04
Identity = 51/205 (24.88%), Postives = 92/205 (44.88%), Query Frame = 0

Query: 19  DKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLPAIVIEEAKEKLSSIFGYE 78
           DK++  V  +++ + ++  G   K E L  ++ KNY +   P I   EA   +  +FG +
Sbjct: 224 DKIIDLV--HLLLRKYRVKGLITKAEMLGSVI-KNYEDY-FPEI-FREASVCMQLLFGID 283

Query: 79  LRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKYVEDVDTAHVTGFNFVVVS 138
           ++E+            P  H      SYVL++ L    Y     +  +   +G   +V+ 
Sbjct: 284 VKEVD-----------PTSH------SYVLVTSLNLS-YDGIQCNEQSMPKSGLLIIVLG 343

Query: 139 IVHLAGGKIPEESLWHHLRRMGLSESDEKHPVLGNIKQAV-ELLVQQRYLQKDKINGPEG 198
           ++ + G  IPEE +W  L  MG+    E H + G  K+ + +  VQ++YL   ++ G + 
Sbjct: 344 VIFMEGNCIPEEVMWEVLSIMGVYAGRE-HFLFGEPKRLLTQNWVQEKYLVYRQVPGTDP 403

Query: 199 NITFYELAERAIDGPVSEKIKEYVA 223
               +    RA       K+ EY+A
Sbjct: 404 ACYEFLWGPRAHAETSKMKVLEYIA 404

BLAST of Cp4.1LG07g01320.1 vs. NCBI nr
Match: XP_023538088.1 (melanoma-associated antigen 1 [Cucurbita pepo subsp. pepo] >XP_023538089.1 melanoma-associated antigen 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 445 bits (1145), Expect = 4.66e-157
Identity = 223/223 (100.00%), Postives = 223/223 (100.00%), Query Frame = 0

Query: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60
           MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP
Sbjct: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60

Query: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 120
           AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY
Sbjct: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 120

Query: 121 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSESDEKHPVLGNIKQAVEL 180
           VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSESDEKHPVLGNIKQAVEL
Sbjct: 121 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSESDEKHPVLGNIKQAVEL 180

Query: 181 LVQQRYLQKDKINGPEGNITFYELAERAIDGPVSEKIKEYVAQ 223
           LVQQRYLQKDKINGPEGNITFYELAERAIDGPVSEKIKEYVAQ
Sbjct: 181 LVQQRYLQKDKINGPEGNITFYELAERAIDGPVSEKIKEYVAQ 223

BLAST of Cp4.1LG07g01320.1 vs. NCBI nr
Match: XP_023002674.1 (melanoma-associated antigen 1 [Cucurbita maxima] >XP_023002675.1 melanoma-associated antigen 1 [Cucurbita maxima])

HSP 1 Score: 441 bits (1134), Expect = 2.21e-155
Identity = 221/223 (99.10%), Postives = 222/223 (99.55%), Query Frame = 0

Query: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60
           MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP
Sbjct: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60

Query: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 120
           AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY
Sbjct: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 120

Query: 121 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSESDEKHPVLGNIKQAVEL 180
           VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSE+DEKHPVLGNIKQAVEL
Sbjct: 121 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKQAVEL 180

Query: 181 LVQQRYLQKDKINGPEGNITFYELAERAIDGPVSEKIKEYVAQ 223
           LVQQRYLQK KINGPEGNITFYELAERAIDGPVSEKIKEYVAQ
Sbjct: 181 LVQQRYLQKAKINGPEGNITFYELAERAIDGPVSEKIKEYVAQ 223

BLAST of Cp4.1LG07g01320.1 vs. NCBI nr
Match: XP_022952014.1 (melanoma-associated antigen 2 [Cucurbita moschata] >XP_022952015.1 melanoma-associated antigen 2 [Cucurbita moschata])

HSP 1 Score: 439 bits (1128), Expect = 1.82e-154
Identity = 219/223 (98.21%), Postives = 222/223 (99.55%), Query Frame = 0

Query: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60
           MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP
Sbjct: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60

Query: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 120
           AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY
Sbjct: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 120

Query: 121 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSESDEKHPVLGNIKQAVEL 180
           VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSE+DEKHPVLGNIK+AVEL
Sbjct: 121 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKEAVEL 180

Query: 181 LVQQRYLQKDKINGPEGNITFYELAERAIDGPVSEKIKEYVAQ 223
           LVQQRYLQK KINGP+GNITFYELAERAIDGPVSEKIKEYVAQ
Sbjct: 181 LVQQRYLQKAKINGPDGNITFYELAERAIDGPVSEKIKEYVAQ 223

BLAST of Cp4.1LG07g01320.1 vs. NCBI nr
Match: KAG7020215.1 (Melanoma-associated antigen 8 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 437 bits (1125), Expect = 9.62e-154
Identity = 219/223 (98.21%), Postives = 221/223 (99.10%), Query Frame = 0

Query: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60
           MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP
Sbjct: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60

Query: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 120
           AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY
Sbjct: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 120

Query: 121 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSESDEKHPVLGNIKQAVEL 180
           VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSE+DEKHPVLGNIKQAVEL
Sbjct: 121 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKQAVEL 180

Query: 181 LVQQRYLQKDKINGPEGNITFYELAERAIDGPVSEKIKEYVAQ 223
           LVQQRYLQK K+NG EGNITFYELAERAIDGPVSEKIKEYVAQ
Sbjct: 181 LVQQRYLQKAKVNGSEGNITFYELAERAIDGPVSEKIKEYVAQ 223

BLAST of Cp4.1LG07g01320.1 vs. NCBI nr
Match: KAG6585294.1 (Non-structural maintenance of chromosomes element 3-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 437 bits (1125), Expect = 3.16e-146
Identity = 219/223 (98.21%), Postives = 221/223 (99.10%), Query Frame = 0

Query: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60
           MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP
Sbjct: 553 MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 612

Query: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 120
           AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY
Sbjct: 613 AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 672

Query: 121 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSESDEKHPVLGNIKQAVEL 180
           VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSE+DEKHPVLGNIKQAVEL
Sbjct: 673 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKQAVEL 732

Query: 181 LVQQRYLQKDKINGPEGNITFYELAERAIDGPVSEKIKEYVAQ 223
           LVQQRYLQK K+NG EGNITFYELAERAIDGPVSEKIKEYVAQ
Sbjct: 733 LVQQRYLQKAKVNGSEGNITFYELAERAIDGPVSEKIKEYVAQ 775

BLAST of Cp4.1LG07g01320.1 vs. ExPASy TrEMBL
Match: A0A6J1KPM1 (melanoma-associated antigen 1 OS=Cucurbita maxima OX=3661 GN=LOC111496460 PE=4 SV=1)

HSP 1 Score: 441 bits (1134), Expect = 1.07e-155
Identity = 221/223 (99.10%), Postives = 222/223 (99.55%), Query Frame = 0

Query: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60
           MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP
Sbjct: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60

Query: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 120
           AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY
Sbjct: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 120

Query: 121 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSESDEKHPVLGNIKQAVEL 180
           VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSE+DEKHPVLGNIKQAVEL
Sbjct: 121 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKQAVEL 180

Query: 181 LVQQRYLQKDKINGPEGNITFYELAERAIDGPVSEKIKEYVAQ 223
           LVQQRYLQK KINGPEGNITFYELAERAIDGPVSEKIKEYVAQ
Sbjct: 181 LVQQRYLQKAKINGPEGNITFYELAERAIDGPVSEKIKEYVAQ 223

BLAST of Cp4.1LG07g01320.1 vs. ExPASy TrEMBL
Match: A0A6J1GKJ9 (melanoma-associated antigen 2 OS=Cucurbita moschata OX=3662 GN=LOC111454728 PE=4 SV=1)

HSP 1 Score: 439 bits (1128), Expect = 8.79e-155
Identity = 219/223 (98.21%), Postives = 222/223 (99.55%), Query Frame = 0

Query: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60
           MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP
Sbjct: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60

Query: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 120
           AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY
Sbjct: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 120

Query: 121 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSESDEKHPVLGNIKQAVEL 180
           VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSE+DEKHPVLGNIK+AVEL
Sbjct: 121 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKEAVEL 180

Query: 181 LVQQRYLQKDKINGPEGNITFYELAERAIDGPVSEKIKEYVAQ 223
           LVQQRYLQK KINGP+GNITFYELAERAIDGPVSEKIKEYVAQ
Sbjct: 181 LVQQRYLQKAKINGPDGNITFYELAERAIDGPVSEKIKEYVAQ 223

BLAST of Cp4.1LG07g01320.1 vs. ExPASy TrEMBL
Match: A0A6J1BU01 (melanoma-associated antigen 1 OS=Momordica charantia OX=3673 GN=LOC111004771 PE=4 SV=1)

HSP 1 Score: 412 bits (1058), Expect = 3.94e-144
Identity = 202/223 (90.58%), Postives = 214/223 (95.96%), Query Frame = 0

Query: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60
           MA +GDYLSQIDISNEEKDKLVAE IRYIIFKTHQNSGCPIKREELTQ+VTKNYRNRGLP
Sbjct: 1   MAGAGDYLSQIDISNEEKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLP 60

Query: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 120
           A+VIEEAKEK+SSIFGYELRELQR RPSST+QGH  Q NV D+KSYVLISKLPA++YRKY
Sbjct: 61  AVVIEEAKEKMSSIFGYELRELQRTRPSSTSQGHSSQQNVADTKSYVLISKLPADIYRKY 120

Query: 121 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSESDEKHPVLGNIKQAVEL 180
           VEDV+TAHVTGF FVVVSIVHLAGGKIPEESLWHHLRRMGLSESDEKHPVLGNIKQAVEL
Sbjct: 121 VEDVNTAHVTGFTFVVVSIVHLAGGKIPEESLWHHLRRMGLSESDEKHPVLGNIKQAVEL 180

Query: 181 LVQQRYLQKDKINGPEGNITFYELAERAIDGPVSEKIKEYVAQ 223
           LVQ+RYLQK KINGPEGNITFYELAERA+DGPVSE+IKEYVAQ
Sbjct: 181 LVQKRYLQKAKINGPEGNITFYELAERALDGPVSEEIKEYVAQ 223

BLAST of Cp4.1LG07g01320.1 vs. ExPASy TrEMBL
Match: A0A1S3BBA9 (uncharacterized protein LOC103488065 OS=Cucumis melo OX=3656 GN=LOC103488065 PE=4 SV=1)

HSP 1 Score: 391 bits (1005), Expect = 4.85e-136
Identity = 194/223 (87.00%), Postives = 206/223 (92.38%), Query Frame = 0

Query: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60
           MAS+G++LSQ+DIS EEKDKLVAE IRYIIFKTHQNSGCPIKREELTQ+VTKNYRNRGLP
Sbjct: 1   MASAGEHLSQLDISEEEKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLP 60

Query: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 120
           AIVI+EAK KLSSIFGYELRELQR RPSST Q H  Q NV D+KSYVLISKLPA++YRKY
Sbjct: 61  AIVIDEAKRKLSSIFGYELRELQRTRPSSTGQPHSSQQNVADAKSYVLISKLPADIYRKY 120

Query: 121 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSESDEKHPVLGNIKQAVEL 180
           VEDVDTAHVTGF FVVVSIVHLAGGKIPEESL HHLRRMGLSESDE HPVLGNIK A+EL
Sbjct: 121 VEDVDTAHVTGFTFVVVSIVHLAGGKIPEESLRHHLRRMGLSESDENHPVLGNIKHAIEL 180

Query: 181 LVQQRYLQKDKINGPEGNITFYELAERAIDGPVSEKIKEYVAQ 223
           +VQQRYLQK K+N PEGNITFYELAERA DGPVSEKIKEYVAQ
Sbjct: 181 VVQQRYLQKVKVNAPEGNITFYELAERATDGPVSEKIKEYVAQ 223

BLAST of Cp4.1LG07g01320.1 vs. ExPASy TrEMBL
Match: A0A0A0LL83 (MAGE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G368960 PE=4 SV=1)

HSP 1 Score: 379 bits (973), Expect = 3.38e-131
Identity = 188/223 (84.30%), Postives = 204/223 (91.48%), Query Frame = 0

Query: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60
           MAS+G+Y  ++DIS EEKDKLVAE IRYIIFKTHQNSGCPIKREELTQ+VTKNYRNRGLP
Sbjct: 1   MASAGEY--ELDISEEEKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLP 60

Query: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 120
           AIVI+EAK KLSSIFGYELRELQR RPSST Q H  Q NV D+KSYVLISKLPA++YRKY
Sbjct: 61  AIVIDEAKRKLSSIFGYELRELQRTRPSSTGQPHSSQQNVGDAKSYVLISKLPADIYRKY 120

Query: 121 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSESDEKHPVLGNIKQAVEL 180
           VEDV+TAHVTGF FV++SIVHLAGGKIPEESL HHL+RMGLSESDE HPVLGNIK A+EL
Sbjct: 121 VEDVNTAHVTGFTFVIISIVHLAGGKIPEESLRHHLKRMGLSESDENHPVLGNIKHAIEL 180

Query: 181 LVQQRYLQKDKINGPEGNITFYELAERAIDGPVSEKIKEYVAQ 223
           +VQQRYLQK K+N PEGNITFYELAERA DGPVSEKIKEYVAQ
Sbjct: 181 VVQQRYLQKVKVNAPEGNITFYELAERATDGPVSEKIKEYVAQ 221

BLAST of Cp4.1LG07g01320.1 vs. TAIR 10
Match: AT1G34770.1 (CONTAINS InterPro DOMAIN/s: MAGE protein (InterPro:IPR002190); Has 1274 Blast hits to 1260 proteins in 85 species: Archae - 0; Bacteria - 0; Metazoa - 1104; Fungi - 45; Plants - 49; Viruses - 0; Other Eukaryotes - 76 (source: NCBI BLink). )

HSP 1 Score: 300.8 bits (769), Expect = 1.0e-81
Identity = 149/224 (66.52%), Postives = 184/224 (82.14%), Query Frame = 0

Query: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60
           MA   D LSQ DIS EE DKLV+EVIR+I+FK HQ+SG PIKRE+LTQIVTKNYR R L 
Sbjct: 1   MADEEDSLSQFDISKEETDKLVSEVIRFILFKFHQSSGTPIKREDLTQIVTKNYRQRNLA 60

Query: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQ-HNVVDSKSYVLISKLPAEVYRK 120
             VI EAK+KLS++FGY+L+ELQRAR SST Q   PQ  + VDSKSYVL+S+LP EV++K
Sbjct: 61  THVINEAKKKLSNVFGYDLKELQRARSSSTGQSRLPQSQSSVDSKSYVLVSELPLEVFKK 120

Query: 121 YVEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSESDEKHPVLGNIKQAVE 180
           +V D  T+ VTGF FVV++IV LAGGKIPEE+LWHHL+RMGL E+DE +PV GN KQ +E
Sbjct: 121 HVVDETTSPVTGFTFVVLAIVQLAGGKIPEETLWHHLKRMGLHENDEHNPVFGNNKQTLE 180

Query: 181 LLVQQRYLQKDKINGPEGNITFYELAERAIDGPVSEKIKEYVAQ 224
            LVQQR+LQK+K++GPEG+  FY+LAERA+D  VSEK+K+Y++Q
Sbjct: 181 TLVQQRFLQKEKVSGPEGSTLFYDLAERALDPQVSEKVKDYISQ 224

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P433614.5e-1026.79Melanoma-associated antigen 8 OS=Homo sapiens OX=9606 GN=MAGEA8 PE=1 SV=2[more]
P433632.2e-0925.95Melanoma-associated antigen 10 OS=Homo sapiens OX=9606 GN=MAGEA10 PE=2 SV=2[more]
P433552.9e-0928.29Melanoma-associated antigen 1 OS=Homo sapiens OX=9606 GN=MAGEA1 PE=1 SV=1[more]
P433641.3e-0424.88Melanoma-associated antigen 11 OS=Homo sapiens OX=9606 GN=MAGEA11 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
XP_023538088.14.66e-157100.00melanoma-associated antigen 1 [Cucurbita pepo subsp. pepo] >XP_023538089.1 melan... [more]
XP_023002674.12.21e-15599.10melanoma-associated antigen 1 [Cucurbita maxima] >XP_023002675.1 melanoma-associ... [more]
XP_022952014.11.82e-15498.21melanoma-associated antigen 2 [Cucurbita moschata] >XP_022952015.1 melanoma-asso... [more]
KAG7020215.19.62e-15498.21Melanoma-associated antigen 8 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6585294.13.16e-14698.21Non-structural maintenance of chromosomes element 3-like protein, partial [Cucur... [more]
Match NameE-valueIdentityDescription
A0A6J1KPM11.07e-15599.10melanoma-associated antigen 1 OS=Cucurbita maxima OX=3661 GN=LOC111496460 PE=4 S... [more]
A0A6J1GKJ98.79e-15598.21melanoma-associated antigen 2 OS=Cucurbita moschata OX=3662 GN=LOC111454728 PE=4... [more]
A0A6J1BU013.94e-14490.58melanoma-associated antigen 1 OS=Momordica charantia OX=3673 GN=LOC111004771 PE=... [more]
A0A1S3BBA94.85e-13687.00uncharacterized protein LOC103488065 OS=Cucumis melo OX=3656 GN=LOC103488065 PE=... [more]
A0A0A0LL833.38e-13184.30MAGE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G368960 PE=4 S... [more]
Match NameE-valueIdentityDescription
AT1G34770.11.0e-8166.52CONTAINS InterPro DOMAIN/s: MAGE protein (InterPro:IPR002190); Has 1274 Blast hi... [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002190MAGE homology domainSMARTSM01373MAGE_2coord: 25..217
e-value: 2.0E-30
score: 117.1
IPR002190MAGE homology domainPFAMPF01454MAGEcoord: 25..208
e-value: 7.1E-38
score: 130.4
IPR002190MAGE homology domainPROSITEPS50838MAGEcoord: 18..224
score: 15.306047
IPR041899MAGE homology domain, winged helix WH2 motifGENE3D1.10.10.1210MAGE homology domain, winged helix WH2 motifcoord: 118..242
e-value: 1.3E-18
score: 68.9
IPR041898MAGE homology domain, winged helix WH1 motifGENE3D1.10.10.1200MAGE homology domain, winged helix WH1 motifcoord: 12..114
e-value: 1.2E-23
score: 84.8
NoneNo IPR availablePANTHERPTHR11736:SF14MAGE PROTEINcoord: 4..224
IPR037445Melanoma-associated antigenPANTHERPTHR11736MELANOMA-ASSOCIATED ANTIGEN MAGE ANTIGENcoord: 4..224

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cp4.1LG07g01320Cp4.1LG07g01320gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG07g01320.1:exon:001Cp4.1LG07g01320.1:exon:001exon
Cp4.1LG07g01320.1:exon:002Cp4.1LG07g01320.1:exon:002exon
Cp4.1LG07g01320.1:exon:003Cp4.1LG07g01320.1:exon:003exon
Cp4.1LG07g01320.1:exon:004Cp4.1LG07g01320.1:exon:004exon
Cp4.1LG07g01320.1:exon:005Cp4.1LG07g01320.1:exon:005exon
Cp4.1LG07g01320.1:exon:006Cp4.1LG07g01320.1:exon:006exon
Cp4.1LG07g01320.1:exon:007Cp4.1LG07g01320.1:exon:007exon


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG07g01320.1:five_prime_utr:001Cp4.1LG07g01320.1:five_prime_utr:001five_prime_UTR
Cp4.1LG07g01320.1:five_prime_utr:002Cp4.1LG07g01320.1:five_prime_utr:002five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG07g01320.1:cds:001Cp4.1LG07g01320.1:cds:001CDS
Cp4.1LG07g01320.1:cds:002Cp4.1LG07g01320.1:cds:002CDS
Cp4.1LG07g01320.1:cds:003Cp4.1LG07g01320.1:cds:003CDS
Cp4.1LG07g01320.1:cds:004Cp4.1LG07g01320.1:cds:004CDS
Cp4.1LG07g01320.1:cds:005Cp4.1LG07g01320.1:cds:005CDS
Cp4.1LG07g01320.1:cds:006Cp4.1LG07g01320.1:cds:006CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cp4.1LG07g01320.1Cp4.1LG07g01320.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005634 nucleus