Cp4.1LG02g03090.1 (mRNA) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG02g03090.1
Type	mRNA
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	1-aminocyclopropane-1-carboxylate oxidase
Location	Cp4.1LG02: 3394347 .. 3395607 (+)
Sequence length	939
RNA-Seq Expression	Cp4.1LG02g03090.1
Synteny	Cp4.1LG02g03090.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAATCCCAGTCATTGATTTCTCAAAGCTTGATGGAGAAGAAAGGGCCAAGACATTGGCTCAGATAGCTAATGGCTGTGAAGAATGGGGATTCTTTCAGGTAGTTCGCACAAACTTATTAGTTATTCTATTCATTGAGTAACGGTTTTTCAAGAGGTAGCTATTTGAATTTATCTTTTTAACGGAGATATAATGTCTTAACCAGTTGAGTGAGTTATACTCAAGTTGGTCAATGTTTTCATGCAAGAGCGCTAATTAAATGTTCTATAATATTGTGGACTAAAATATATGTTACCTGAATTTCAGCTGGTGAACCATGGGATTCCAGAAAAGCTCCTGGAGAGGGTGAAGAAGGTTTGTTCAGAGTGCTACAAGCTGGAAAGAGAAGAGAATTTCAAGAGTTCAAAACCTGTGAAGCTCCTGAATGATTTGCTGGAACGCAAGAGCGGCGAGAAGCTGGAAAATCTTGATTGGGAAGATGTCTTCCTTCTCCATGATAACAACGAATGGCCCTCCAACATTCCTGGATTCAAGTAAGCATGCACCTACACTTCAAACTTTATGCTTTTTCGTGAAAATATATCATGCAATCAATTTTTCTTACAAACAGAGAGTAGGTTTTGAAAGCAACTCACGAATATTGTGGCAGGGAAACCATGGCAGAGTATAGATCGGAGCTGAAGAAGCTAGCTGCAAAGGTGATGGCAGTGATGGATGAGAACTTGGGTTTACCTGAGGGATACATAAAGGCTGCCTGCAATGGTGGCGATGGGCTGGACAAGGCGTTCTTTGGAACCAAAGTCAGTCACTACCCACCATGCCCACACCCGGAGTTGGTGAACGGCCTCCGTGCGCATACCGATGCTGGTGGTGTCATCTTGCTCTTCCAGGATGACGAGGTGGGAGGCCTTCAAATCCTGAAAGATGGGCAGTGGATAGATGTCCAGCCATTGCCGAACTCCATAGTCATCAACACAGGTGATCAGATTGAGGTCCTGAGCAATGGAAGATACAAGAGTGTTTGGCACCGGGTTCTGCCCTCCCCAAATGGGAATAGGAGATCCCTTGCTTCGTTTTACAACCCATCAATGGAAGCCATCATAGCTCCGGCACCAAAGCTGGTGAATAAAGCAAACCAGGAGGTGGAAAAAGGATATCCCAAGTTTGTGTTTGGAGACTACATGTCGGTTTATGCTGAGCAGAAGTTCCTTCCAAAGGAACCAAGGTTTCAAGCTGTCAGGGCAATGCGAGGGACATGA

mRNA sequence

ATGGCAATCCCAGTCATTGATTTCTCAAAGCTTGATGGAGAAGAAAGGGCCAAGACATTGGCTCAGATAGCTAATGGCTGTGAAGAATGGGGATTCTTTCAGCTGGTGAACCATGGGATTCCAGAAAAGCTCCTGGAGAGGGTGAAGAAGGTTTGTTCAGAGTGCTACAAGCTGGAAAGAGAAGAGAATTTCAAGAGTTCAAAACCTGTGAAGCTCCTGAATGATTTGCTGGAACGCAAGAGCGGCGAGAAGCTGGAAAATCTTGATTGGGAAGATGTCTTCCTTCTCCATGATAACAACGAATGGCCCTCCAACATTCCTGGATTCAAGGAAACCATGGCAGAGTATAGATCGGAGCTGAAGAAGCTAGCTGCAAAGGTGATGGCAGTGATGGATGAGAACTTGGGTTTACCTGAGGGATACATAAAGGCTGCCTGCAATGGTGGCGATGGGCTGGACAAGGCGTTCTTTGGAACCAAAGTCAGTCACTACCCACCATGCCCACACCCGGAGTTGGTGAACGGCCTCCGTGCGCATACCGATGCTGGTGGTGTCATCTTGCTCTTCCAGGATGACGAGGTGGGAGGCCTTCAAATCCTGAAAGATGGGCAGTGGATAGATGTCCAGCCATTGCCGAACTCCATAGTCATCAACACAGGTGATCAGATTGAGGTCCTGAGCAATGGAAGATACAAGAGTGTTTGGCACCGGGTTCTGCCCTCCCCAAATGGGAATAGGAGATCCCTTGCTTCGTTTTACAACCCATCAATGGAAGCCATCATAGCTCCGGCACCAAAGCTGGTGAATAAAGCAAACCAGGAGGTGGAAAAAGGATATCCCAAGTTTGTGTTTGGAGACTACATGTCGGTTTATGCTGAGCAGAAGTTCCTTCCAAAGGAACCAAGGTTTCAAGCTGTCAGGGCAATGCGAGGGACATGA

Coding sequence (CDS)

ATGGCAATCCCAGTCATTGATTTCTCAAAGCTTGATGGAGAAGAAAGGGCCAAGACATTGGCTCAGATAGCTAATGGCTGTGAAGAATGGGGATTCTTTCAGCTGGTGAACCATGGGATTCCAGAAAAGCTCCTGGAGAGGGTGAAGAAGGTTTGTTCAGAGTGCTACAAGCTGGAAAGAGAAGAGAATTTCAAGAGTTCAAAACCTGTGAAGCTCCTGAATGATTTGCTGGAACGCAAGAGCGGCGAGAAGCTGGAAAATCTTGATTGGGAAGATGTCTTCCTTCTCCATGATAACAACGAATGGCCCTCCAACATTCCTGGATTCAAGGAAACCATGGCAGAGTATAGATCGGAGCTGAAGAAGCTAGCTGCAAAGGTGATGGCAGTGATGGATGAGAACTTGGGTTTACCTGAGGGATACATAAAGGCTGCCTGCAATGGTGGCGATGGGCTGGACAAGGCGTTCTTTGGAACCAAAGTCAGTCACTACCCACCATGCCCACACCCGGAGTTGGTGAACGGCCTCCGTGCGCATACCGATGCTGGTGGTGTCATCTTGCTCTTCCAGGATGACGAGGTGGGAGGCCTTCAAATCCTGAAAGATGGGCAGTGGATAGATGTCCAGCCATTGCCGAACTCCATAGTCATCAACACAGGTGATCAGATTGAGGTCCTGAGCAATGGAAGATACAAGAGTGTTTGGCACCGGGTTCTGCCCTCCCCAAATGGGAATAGGAGATCCCTTGCTTCGTTTTACAACCCATCAATGGAAGCCATCATAGCTCCGGCACCAAAGCTGGTGAATAAAGCAAACCAGGAGGTGGAAAAAGGATATCCCAAGTTTGTGTTTGGAGACTACATGTCGGTTTATGCTGAGCAGAAGTTCCTTCCAAAGGAACCAAGGTTTCAAGCTGTCAGGGCAATGCGAGGGACATGA

Protein sequence

MAIPVIDFSKLDGEERAKTLAQIANGCEEWGFFQLVNHGIPEKLLERVKKVCSECYKLEREENFKSSKPVKLLNDLLERKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSELKKLAAKVMAVMDENLGLPEGYIKAACNGGDGLDKAFFGTKVSHYPPCPHPELVNGLRAHTDAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLPSPNGNRRSLASFYNPSMEAIIAPAPKLVNKANQEVEKGYPKFVFGDYMSVYAEQKFLPKEPRFQAVRAMRGT

Homology

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BLAST of Cp4.1LG02g03090.1 vs. ExPASy Swiss-Prot
Match: Q0WPW4 (1-aminocyclopropane-1-carboxylate oxidase 5 OS=Arabidopsis thaliana OX=3702 GN=At1g77330 PE=2 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 1.8e-133
Identity = 231/313 (73.80%), Postives = 263/313 (84.03%), Query Frame = 0


Query: 1   MAIPVIDFSKLDGEERAKTLAQIANGCEEWGFFQLVNHGIPEKLLERVKKVCSECYKLER 60
           MAIPVIDFSKL+GEER KTL++IA  CEEWGFFQLVNHGIP +LL +VKK+ S+CYK ER
Sbjct: 1   MAIPVIDFSKLNGEEREKTLSEIARACEEWGFFQLVNHGIPLELLNKVKKLSSDCYKTER 60

Query: 61  EENFKSSKPVKLLNDLLERKSGEKLENLDWEDVFLL--HDNNEWPSNIPGFKETMAEYRS 120
           EE FK+S PVKLLN+L+++ SGEKLEN+DWEDVF L  H+ NEWPSNI   KETM EYR 
Sbjct: 61  EEAFKTSNPVKLLNELVQKNSGEKLENVDWEDVFTLLDHNQNEWPSNI---KETMGEYRE 120

Query: 121 ELKKLAAKVMAVMDENLGLPEGYIKAACNGG--DGLDKAFFGTKVSHYPPCPHPELVNGL 180
           E++KLA+K+M VMDENLGLP+GYIK A N G  DG + AFFGTKVSHYPPCPHPELVNGL
Sbjct: 121 EVRKLASKMMEVMDENLGLPKGYIKKAFNEGMEDGEETAFFGTKVSHYPPCPHPELVNGL 180

Query: 181 RAHTDAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWH 240
           RAHTDAGGV+LLFQDDE  GLQ+LKDG+WIDVQPLPN+IVINTGDQIEVLSNGRYKS WH
Sbjct: 181 RAHTDAGGVVLLFQDDEYDGLQVLKDGEWIDVQPLPNAIVINTGDQIEVLSNGRYKSAWH 240

Query: 241 RVLPSPNGNRRSLASFYNPSMEAIIAPAPKLVNKANQEVEKGYPKFVFGDYMSVYAEQKF 300
           RVL    GNRRS+ASFYNPS +A I PA     + +   EK YPKFVFGDYM VYA QKF
Sbjct: 241 RVLAREEGNRRSIASFYNPSYKAAIGPAAVAEEEGS---EKKYPKFVFGDYMDVYANQKF 300

Query: 301 LPKEPRFQAVRAM 310
           +PKEPRF AV+++
Sbjct: 301 MPKEPRFLAVKSL 307

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BLAST of Cp4.1LG02g03090.1 vs. ExPASy Swiss-Prot
Match: Q9FR99 (1-aminocyclopropane-1-carboxylate oxidase OS=Musa acuminata OX=4641 GN=MAO1B PE=3 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 6.6e-128
Identity = 223/311 (71.70%), Postives = 262/311 (84.24%), Query Frame = 0


Query: 1   MAIPVIDFSKLDGEERAKTLAQIANGCEEWGFFQLVNHGIPEKLLERVKKVCSECYKLER 60
           MAIPVIDFSKLDG+ERA+T+A+IANGCEEWGFFQLVNHGIP +LLERVKKV SECYKL R
Sbjct: 1   MAIPVIDFSKLDGKERAETMARIANGCEEWGFFQLVNHGIPVELLERVKKVSSECYKL-R 60

Query: 61  EENFKSSKPVKLLNDLLERKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL 120
           EE F+ SKPV+LL+ L++   G++L+N+DWEDVF+L D+NEWPSN P F+ETM EYR E+
Sbjct: 61  EERFEGSKPVQLLDTLVKEGDGQRLDNVDWEDVFVLQDDNEWPSNPPDFEETMKEYREEI 120

Query: 121 KKLAAKVMAVMDENLGLPEGYIKAACNGGDGLDKAFFGTKVSHYPPCPHPELVNGLRAHT 180
           +KLA K+M VMDENLG  +G IK A + GDG    FFGTKVSHYPPCP  +LV GLRAHT
Sbjct: 121 RKLAEKMMEVMDENLGFEKGCIKKAFS-GDGQHPPFFGTKVSHYPPCPRLDLVKGLRAHT 180

Query: 181 DAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLP 240
           DAGGVILLFQDD+VGGLQ+LKDG+WIDVQPL ++IVINTGDQIEVLSNGRYKS WHRVL 
Sbjct: 181 DAGGVILLFQDDQVGGLQMLKDGRWIDVQPLADAIVINTGDQIEVLSNGRYKSAWHRVLA 240

Query: 241 SPNGNRRSLASFYNPSMEAIIAPAPKLVNKANQEVEKG--YPKFVFGDYMSVYAEQKFLP 300
           + +GNRRS+ASFYNPS++A IAPA      A +E      YPKF+FGDYM VYA+QK+ P
Sbjct: 241 TSHGNRRSIASFYNPSLKATIAPA---AGAATEEAAPPALYPKFLFGDYMDVYAKQKYEP 300

Query: 301 KEPRFQAVRAM 310
           KEPRF+AVRA+
Sbjct: 301 KEPRFEAVRAI 306

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BLAST of Cp4.1LG02g03090.1 vs. ExPASy Swiss-Prot
Match: P31237 (1-aminocyclopropane-1-carboxylate oxidase OS=Actinidia deliciosa OX=3627 GN=ACO PE=2 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 6.0e-89
Identity = 160/313 (51.12%), Postives = 218/313 (69.65%), Query Frame = 0


Query: 2   AIPVIDFSKLDGEERAKTLAQIANGCEEWGFFQLVNHGIPEKLLERVKKVCSECYKLERE 61
           A PVID  KL+GEERA T+ +I + CE WGFF+LVNHGI  +L++ V+++  E Y    E
Sbjct: 3   AFPVIDMEKLNGEERAPTMEKIKDACENWGFFELVNHGISHELMDTVERLTKEHYNKCME 62

Query: 62  ENFKSSKPVKLLNDLLERKSGEKLENLDWEDVFLLH-----DNNEWPSNIPGFKETMAEY 121
           + FK     K L  +       ++ +LDWE  F L      + +E P      ++ M E+
Sbjct: 63  QRFKEMVATKGLEAV-----QSEINDLDWESTFFLRHLPVSNISEIPDLEQDHRKAMKEF 122

Query: 122 RSELKKLAAKVMAVMDENLGLPEGYIKAACNGGDGLDKAFFGTKVSHYPPCPHPELVNGL 181
             +L+KLA +++ ++ EN+GL +GY+K A  G  G     FGTKVS+YPPCP PEL+ GL
Sbjct: 123 AEKLEKLAEQLLDLLCENVGLEKGYLKKAFYGSKG---PTFGTKVSNYPPCPRPELIKGL 182

Query: 182 RAHTDAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWH 241
           RAHTDAGG+ILLFQD++V GLQ+LKDG+WIDV P+ +SIVIN GDQ+EV++NG+YKSV H
Sbjct: 183 RAHTDAGGIILLFQDNKVSGLQLLKDGEWIDVPPMKHSIVINIGDQLEVITNGKYKSVMH 242

Query: 242 RVLPSPNGNRRSLASFYNPSMEAIIAPAPKLVNKANQEVEKGYPKFVFGDYMSVYAEQKF 301
           RV+  P+GNR S+ASFYNP  +A++ PAP LV+K   + ++ YPKFVF DYM +YA  KF
Sbjct: 243 RVIAQPDGNRMSIASFYNPGSDAVMYPAPALVDKEEDQQKQVYPKFVFEDYMKLYAGLKF 302

Query: 302 LPKEPRFQAVRAM 310
             KEPRF+A++AM
Sbjct: 303 QAKEPRFEAMKAM 307

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BLAST of Cp4.1LG02g03090.1 vs. ExPASy Swiss-Prot
Match: Q00985 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Malus domestica OX=3750 PE=1 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 7.4e-87
Identity = 157/314 (50.00%), Postives = 218/314 (69.43%), Query Frame = 0


Query: 4   PVIDFSKLDGEERAKTLAQIANGCEEWGFFQLVNHGIPEKLLERVKKVCSECYKLEREEN 63
           PV+D S ++GEERA TL +I + CE WGFF+LVNHG+  +LL+ V+K+  + YK   E+ 
Sbjct: 5   PVVDLSLVNGEERAATLEKINDACENWGFFELVNHGMSTELLDTVEKMTKDHYKKTMEQR 64

Query: 64  FKSSKPVKLLNDLLERKSGEKLENLDWEDVFLLH-----DNNEWPSNIPGFKETMAEYRS 123
           FK     K L+D+       ++ +LDWE  F L      + +E P     +++TM E+  
Sbjct: 65  FKEMVAAKGLDDV-----QSEIHDLDWESTFFLRHLPSSNISEIPDLEEEYRKTMKEFAV 124

Query: 124 ELKKLAAKVMAVMDENLGLPEGYIKAACNGGDGLDKAFFGTKVSHYPPCPHPELVNGLRA 183
           EL+KLA K++ ++ ENLGL +GY+K    G  G +   FGTKVS+YPPCP P+L+ GLRA
Sbjct: 125 ELEKLAEKLLDLLCENLGLEKGYLKKVFYGSKGPN---FGTKVSNYPPCPKPDLIKGLRA 184

Query: 184 HTDAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRV 243
           H+DAGG+ILLFQDD+V GLQ+LKDG+W+DV P+ +SIVIN GDQIEV++NG+YKSV HRV
Sbjct: 185 HSDAGGIILLFQDDKVSGLQLLKDGEWVDVPPMHHSIVINLGDQIEVITNGKYKSVMHRV 244

Query: 244 LPSPNGNRRSLASFYNPSMEAIIAPAPKLVNKANQEVEKGYPKFVFGDYMSVYAEQKFLP 303
           +   +G R S+ASFYNP  ++ I+PAP ++ K  ++    YPKFVF DYM +Y+  KF  
Sbjct: 245 IAQSDGTRMSIASFYNPGNDSFISPAPAVLEKKTEDAPT-YPKFVFDDYMKLYSGLKFQA 304

Query: 304 KEPRFQAVRAMRGT 313
           KEPRF+A++A   T
Sbjct: 305 KEPRFEAMKAKEST 309

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BLAST of Cp4.1LG02g03090.1 vs. ExPASy Swiss-Prot
Match: Q08506 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Petunia hybrida OX=4102 GN=ACO1 PE=1 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 2.1e-86
Identity = 157/311 (50.48%), Postives = 214/311 (68.81%), Query Frame = 0


Query: 4   PVIDFSKLDGEERAKTLAQIANGCEEWGFFQLVNHGIPEKLLERVKKVCSECYKLEREEN 63
           P+I   K++G ERA T+  I + CE WGFF+LVNHGIP ++++ V+K+    YK   E+ 
Sbjct: 5   PIISLDKVNGVERAATMEMIKDACENWGFFELVNHGIPREVMDTVEKMTKGHYKKCMEQR 64

Query: 64  FKSSKPVKLLNDLLERKSGEKLENLDWEDVFLL-----HDNNEWPSNIPGFKETMAEYRS 123
           FK     K L  +       ++ ++DWE  F L      + +E P     ++E M ++  
Sbjct: 65  FKELVASKALEGV-----QAEVTDMDWESTFFLKHLPISNISEVPDLDEEYREVMRDFAK 124

Query: 124 ELKKLAAKVMAVMDENLGLPEGYIKAACNGGDGLDKAFFGTKVSHYPPCPHPELVNGLRA 183
            L+KLA +++ ++ ENLGL +GY+K A  G  G +   FGTKVS+YPPCP P+L+ GLRA
Sbjct: 125 RLEKLAEELLDLLCENLGLEKGYLKNAFYGSKGPN---FGTKVSNYPPCPKPDLIKGLRA 184

Query: 184 HTDAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRV 243
           HTDAGG+ILLFQDD+V GLQ+LKDGQWIDV P+ +SIV+N GDQ+EV++NG+YKSV HRV
Sbjct: 185 HTDAGGIILLFQDDKVSGLQLLKDGQWIDVPPMRHSIVVNLGDQLEVITNGKYKSVMHRV 244

Query: 244 LPSPNGNRRSLASFYNPSMEAIIAPAPKLVNKANQEVEKGYPKFVFGDYMSVYAEQKFLP 303
           +   +G R SLASFYNP  +A+I PAP LV K  +E ++ YPKFVF DYM +YA  KF  
Sbjct: 245 IAQKDGARMSLASFYNPGSDAVIYPAPALVEKEAEENKQVYPKFVFDDYMKLYAGLKFQA 304

Query: 304 KEPRFQAVRAM 310
           KEPRF+A++AM
Sbjct: 305 KEPRFEAMKAM 307

[Close]

BLAST of Cp4.1LG02g03090.1 vs. NCBI nr
Match: XP_023523759.1 (1-aminocyclopropane-1-carboxylate oxidase 5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 645 bits (1664), Expect = 8.01e-234
Identity = 312/312 (100.00%), Postives = 312/312 (100.00%), Query Frame = 0


Query: 1   MAIPVIDFSKLDGEERAKTLAQIANGCEEWGFFQLVNHGIPEKLLERVKKVCSECYKLER 60
           MAIPVIDFSKLDGEERAKTLAQIANGCEEWGFFQLVNHGIPEKLLERVKKVCSECYKLER
Sbjct: 1   MAIPVIDFSKLDGEERAKTLAQIANGCEEWGFFQLVNHGIPEKLLERVKKVCSECYKLER 60

Query: 61  EENFKSSKPVKLLNDLLERKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL 120
           EENFKSSKPVKLLNDLLERKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL
Sbjct: 61  EENFKSSKPVKLLNDLLERKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL 120

Query: 121 KKLAAKVMAVMDENLGLPEGYIKAACNGGDGLDKAFFGTKVSHYPPCPHPELVNGLRAHT 180
           KKLAAKVMAVMDENLGLPEGYIKAACNGGDGLDKAFFGTKVSHYPPCPHPELVNGLRAHT
Sbjct: 121 KKLAAKVMAVMDENLGLPEGYIKAACNGGDGLDKAFFGTKVSHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLP 240
           DAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLP
Sbjct: 181 DAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLP 240

Query: 241 SPNGNRRSLASFYNPSMEAIIAPAPKLVNKANQEVEKGYPKFVFGDYMSVYAEQKFLPKE 300
           SPNGNRRSLASFYNPSMEAIIAPAPKLVNKANQEVEKGYPKFVFGDYMSVYAEQKFLPKE
Sbjct: 241 SPNGNRRSLASFYNPSMEAIIAPAPKLVNKANQEVEKGYPKFVFGDYMSVYAEQKFLPKE 300

Query: 301 PRFQAVRAMRGT 312
           PRFQAVRAMRGT
Sbjct: 301 PRFQAVRAMRGT 312

[Close]

BLAST of Cp4.1LG02g03090.1 vs. NCBI nr
Match: XP_022941440.1 (1-aminocyclopropane-1-carboxylate oxidase 5-like [Cucurbita moschata])

HSP 1 Score: 636 bits (1640), Expect = 3.65e-230
Identity = 307/312 (98.40%), Postives = 310/312 (99.36%), Query Frame = 0


Query: 1   MAIPVIDFSKLDGEERAKTLAQIANGCEEWGFFQLVNHGIPEKLLERVKKVCSECYKLER 60
           MAIPVIDFSKLDGEER KTLAQIANGCEEWGFFQLVNHGIPE+LLERVKKVCSECY+LER
Sbjct: 1   MAIPVIDFSKLDGEERVKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVCSECYELER 60

Query: 61  EENFKSSKPVKLLNDLLERKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL 120
           EENFKSSKPVKLLNDLLERKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL
Sbjct: 61  EENFKSSKPVKLLNDLLERKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL 120

Query: 121 KKLAAKVMAVMDENLGLPEGYIKAACNGGDGLDKAFFGTKVSHYPPCPHPELVNGLRAHT 180
           KKLAAKVMAVMDENLGLPEGYIKAA NGGDGLDKAFFGTKVSHYPPCPHPELVNGLRAHT
Sbjct: 121 KKLAAKVMAVMDENLGLPEGYIKAAFNGGDGLDKAFFGTKVSHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLP 240
           DAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLP
Sbjct: 181 DAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLP 240

Query: 241 SPNGNRRSLASFYNPSMEAIIAPAPKLVNKANQEVEKGYPKFVFGDYMSVYAEQKFLPKE 300
           SPNGNRRSLASFYNPSMEAIIAPAPKLVNKANQEVEKGYPKFVFGDYMS+YAEQKFLPKE
Sbjct: 241 SPNGNRRSLASFYNPSMEAIIAPAPKLVNKANQEVEKGYPKFVFGDYMSIYAEQKFLPKE 300

Query: 301 PRFQAVRAMRGT 312
           PRFQAVRAMRGT
Sbjct: 301 PRFQAVRAMRGT 312

[Close]

BLAST of Cp4.1LG02g03090.1 vs. NCBI nr
Match: KAG7031659.1 (1-aminocyclopropane-1-carboxylate oxidase 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 636 bits (1640), Expect = 3.65e-230
Identity = 307/312 (98.40%), Postives = 310/312 (99.36%), Query Frame = 0


Query: 1   MAIPVIDFSKLDGEERAKTLAQIANGCEEWGFFQLVNHGIPEKLLERVKKVCSECYKLER 60
           MAIPVIDFSKLDGEER KTLAQIANGCEEWGFFQLVNHGIPE+LLERVKKVCSECYKLER
Sbjct: 1   MAIPVIDFSKLDGEERVKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVCSECYKLER 60

Query: 61  EENFKSSKPVKLLNDLLERKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL 120
           EENFKSSKPVKLLNDLLERKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL
Sbjct: 61  EENFKSSKPVKLLNDLLERKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL 120

Query: 121 KKLAAKVMAVMDENLGLPEGYIKAACNGGDGLDKAFFGTKVSHYPPCPHPELVNGLRAHT 180
           KKLAAKVMAVMDENLGLPEGYIKAA NGGDGLD+AFFGTKVSHYPPCPHPELVNGLRAHT
Sbjct: 121 KKLAAKVMAVMDENLGLPEGYIKAAFNGGDGLDEAFFGTKVSHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLP 240
           DAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLP
Sbjct: 181 DAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLP 240

Query: 241 SPNGNRRSLASFYNPSMEAIIAPAPKLVNKANQEVEKGYPKFVFGDYMSVYAEQKFLPKE 300
           SPNGNRRSLASFYNPSMEAIIAPAPKLVNKANQEVEKGYPKFVFGDYMS+YAEQKFLPKE
Sbjct: 241 SPNGNRRSLASFYNPSMEAIIAPAPKLVNKANQEVEKGYPKFVFGDYMSIYAEQKFLPKE 300

Query: 301 PRFQAVRAMRGT 312
           PRFQAVRAMRGT
Sbjct: 301 PRFQAVRAMRGT 312

[Close]

BLAST of Cp4.1LG02g03090.1 vs. NCBI nr
Match: XP_022981033.1 (1-aminocyclopropane-1-carboxylate oxidase 5-like [Cucurbita maxima])

HSP 1 Score: 616 bits (1588), Expect = 3.09e-222
Identity = 298/312 (95.51%), Postives = 305/312 (97.76%), Query Frame = 0


Query: 1   MAIPVIDFSKLDGEERAKTLAQIANGCEEWGFFQLVNHGIPEKLLERVKKVCSECYKLER 60
           MAIPVIDFSKLDGEERA TLAQIANGCEEWGFFQLVNHGIPE+LLERVKKVCSECYKLER
Sbjct: 1   MAIPVIDFSKLDGEERANTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVCSECYKLER 60

Query: 61  EENFKSSKPVKLLNDLLERKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL 120
           EENFKSSKPVKLLNDLLERKSGEKLE+LDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL
Sbjct: 61  EENFKSSKPVKLLNDLLERKSGEKLESLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL 120

Query: 121 KKLAAKVMAVMDENLGLPEGYIKAACNGGDGLDKAFFGTKVSHYPPCPHPELVNGLRAHT 180
           KKLAAKVMAVMDENLGLPEGYI+AA NGG GLDKAFFGTKVSHYPPCPHPELVNGLRAHT
Sbjct: 121 KKLAAKVMAVMDENLGLPEGYIQAAFNGGGGLDKAFFGTKVSHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLP 240
           DAGGVILLFQDDEVGGLQILKDGQWIDVQPL NSIVINTGDQIEVLSNGRYKSVWHRVLP
Sbjct: 181 DAGGVILLFQDDEVGGLQILKDGQWIDVQPLQNSIVINTGDQIEVLSNGRYKSVWHRVLP 240

Query: 241 SPNGNRRSLASFYNPSMEAIIAPAPKLVNKANQEVEKGYPKFVFGDYMSVYAEQKFLPKE 300
           SPNGNRRSLASFYNPSMEAII PA ++VNKANQEVE+GYPKFVFGDYMS+YAEQKFLPKE
Sbjct: 241 SPNGNRRSLASFYNPSMEAIIGPAAQVVNKANQEVEEGYPKFVFGDYMSIYAEQKFLPKE 300

Query: 301 PRFQAVRAMRGT 312
           PRFQAVRAMR T
Sbjct: 301 PRFQAVRAMRET 312

[Close]

BLAST of Cp4.1LG02g03090.1 vs. NCBI nr
Match: XP_022936989.1 (1-aminocyclopropane-1-carboxylate oxidase 5-like isoform X1 [Cucurbita moschata] >XP_022936990.1 1-aminocyclopropane-1-carboxylate oxidase 5-like isoform X2 [Cucurbita moschata] >KAG6591869.1 1-aminocyclopropane-1-carboxylate oxidase 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 595 bits (1535), Expect = 3.32e-214
Identity = 285/309 (92.23%), Postives = 301/309 (97.41%), Query Frame = 0


Query: 1   MAIPVIDFSKLDGEERAKTLAQIANGCEEWGFFQLVNHGIPEKLLERVKKVCSECYKLER 60
           MAIPVIDFSKLDGEERAKTL QIANGCEEWGFFQLVNHGIPE+LLERVK+VCSECYKLER
Sbjct: 1   MAIPVIDFSKLDGEERAKTLNQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER 60

Query: 61  EENFKSSKPVKLLNDLLERKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL 120
           EE+FKSSKPVKLLNDLLE KSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL
Sbjct: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL 120

Query: 121 KKLAAKVMAVMDENLGLPEGYIKAACNGGDGLDKAFFGTKVSHYPPCPHPELVNGLRAHT 180
           KKLA KVMA+MDENLGLPEGYIKAA NGG+GL+KAFFGTKVSHYPPCPHPELVNGLRAHT
Sbjct: 121 KKLAEKVMALMDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLP 240
           DAGGVILLFQDD+VGGLQILKDGQWIDVQPL NSIVINTGDQIEVLSNG+YKSVWHR+L 
Sbjct: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLHNSIVINTGDQIEVLSNGKYKSVWHRILA 240

Query: 241 SPNGNRRSLASFYNPSMEAIIAPAPKLVNKANQEVEKGYPKFVFGDYMSVYAEQKFLPKE 300
           +PNGNRRS+ASFYNPS+EA IAPA +LV+KANQEVE+GYPKFVFGDYMSVYAEQKFLPKE
Sbjct: 241 TPNGNRRSIASFYNPSLEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300

Query: 301 PRFQAVRAM 309
           PRFQAVRA+
Sbjct: 301 PRFQAVRAV 309

[Close]

BLAST of Cp4.1LG02g03090.1 vs. ExPASy TrEMBL
Match: A0A6J1FTN8 (1-aminocyclopropane-1-carboxylate oxidase 5-like OS=Cucurbita moschata OX=3662 GN=LOC111446734 PE=3 SV=1)

HSP 1 Score: 636 bits (1640), Expect = 1.77e-230
Identity = 307/312 (98.40%), Postives = 310/312 (99.36%), Query Frame = 0


Query: 1   MAIPVIDFSKLDGEERAKTLAQIANGCEEWGFFQLVNHGIPEKLLERVKKVCSECYKLER 60
           MAIPVIDFSKLDGEER KTLAQIANGCEEWGFFQLVNHGIPE+LLERVKKVCSECY+LER
Sbjct: 1   MAIPVIDFSKLDGEERVKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVCSECYELER 60

Query: 61  EENFKSSKPVKLLNDLLERKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL 120
           EENFKSSKPVKLLNDLLERKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL
Sbjct: 61  EENFKSSKPVKLLNDLLERKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL 120

Query: 121 KKLAAKVMAVMDENLGLPEGYIKAACNGGDGLDKAFFGTKVSHYPPCPHPELVNGLRAHT 180
           KKLAAKVMAVMDENLGLPEGYIKAA NGGDGLDKAFFGTKVSHYPPCPHPELVNGLRAHT
Sbjct: 121 KKLAAKVMAVMDENLGLPEGYIKAAFNGGDGLDKAFFGTKVSHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLP 240
           DAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLP
Sbjct: 181 DAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLP 240

Query: 241 SPNGNRRSLASFYNPSMEAIIAPAPKLVNKANQEVEKGYPKFVFGDYMSVYAEQKFLPKE 300
           SPNGNRRSLASFYNPSMEAIIAPAPKLVNKANQEVEKGYPKFVFGDYMS+YAEQKFLPKE
Sbjct: 241 SPNGNRRSLASFYNPSMEAIIAPAPKLVNKANQEVEKGYPKFVFGDYMSIYAEQKFLPKE 300

Query: 301 PRFQAVRAMRGT 312
           PRFQAVRAMRGT
Sbjct: 301 PRFQAVRAMRGT 312

[Close]

BLAST of Cp4.1LG02g03090.1 vs. ExPASy TrEMBL
Match: A0A6J1J108 (1-aminocyclopropane-1-carboxylate oxidase 5-like OS=Cucurbita maxima OX=3661 GN=LOC111480303 PE=3 SV=1)

HSP 1 Score: 616 bits (1588), Expect = 1.50e-222
Identity = 298/312 (95.51%), Postives = 305/312 (97.76%), Query Frame = 0


Query: 1   MAIPVIDFSKLDGEERAKTLAQIANGCEEWGFFQLVNHGIPEKLLERVKKVCSECYKLER 60
           MAIPVIDFSKLDGEERA TLAQIANGCEEWGFFQLVNHGIPE+LLERVKKVCSECYKLER
Sbjct: 1   MAIPVIDFSKLDGEERANTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVCSECYKLER 60

Query: 61  EENFKSSKPVKLLNDLLERKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL 120
           EENFKSSKPVKLLNDLLERKSGEKLE+LDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL
Sbjct: 61  EENFKSSKPVKLLNDLLERKSGEKLESLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL 120

Query: 121 KKLAAKVMAVMDENLGLPEGYIKAACNGGDGLDKAFFGTKVSHYPPCPHPELVNGLRAHT 180
           KKLAAKVMAVMDENLGLPEGYI+AA NGG GLDKAFFGTKVSHYPPCPHPELVNGLRAHT
Sbjct: 121 KKLAAKVMAVMDENLGLPEGYIQAAFNGGGGLDKAFFGTKVSHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLP 240
           DAGGVILLFQDDEVGGLQILKDGQWIDVQPL NSIVINTGDQIEVLSNGRYKSVWHRVLP
Sbjct: 181 DAGGVILLFQDDEVGGLQILKDGQWIDVQPLQNSIVINTGDQIEVLSNGRYKSVWHRVLP 240

Query: 241 SPNGNRRSLASFYNPSMEAIIAPAPKLVNKANQEVEKGYPKFVFGDYMSVYAEQKFLPKE 300
           SPNGNRRSLASFYNPSMEAII PA ++VNKANQEVE+GYPKFVFGDYMS+YAEQKFLPKE
Sbjct: 241 SPNGNRRSLASFYNPSMEAIIGPAAQVVNKANQEVEEGYPKFVFGDYMSIYAEQKFLPKE 300

Query: 301 PRFQAVRAMRGT 312
           PRFQAVRAMR T
Sbjct: 301 PRFQAVRAMRET 312

[Close]

BLAST of Cp4.1LG02g03090.1 vs. ExPASy TrEMBL
Match: A0A6J1FFA0 (1-aminocyclopropane-1-carboxylate oxidase 5-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443415 PE=3 SV=1)

HSP 1 Score: 595 bits (1535), Expect = 1.61e-214
Identity = 285/309 (92.23%), Postives = 301/309 (97.41%), Query Frame = 0


Query: 1   MAIPVIDFSKLDGEERAKTLAQIANGCEEWGFFQLVNHGIPEKLLERVKKVCSECYKLER 60
           MAIPVIDFSKLDGEERAKTL QIANGCEEWGFFQLVNHGIPE+LLERVK+VCSECYKLER
Sbjct: 1   MAIPVIDFSKLDGEERAKTLNQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER 60

Query: 61  EENFKSSKPVKLLNDLLERKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL 120
           EE+FKSSKPVKLLNDLLE KSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL
Sbjct: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL 120

Query: 121 KKLAAKVMAVMDENLGLPEGYIKAACNGGDGLDKAFFGTKVSHYPPCPHPELVNGLRAHT 180
           KKLA KVMA+MDENLGLPEGYIKAA NGG+GL+KAFFGTKVSHYPPCPHPELVNGLRAHT
Sbjct: 121 KKLAEKVMALMDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLP 240
           DAGGVILLFQDD+VGGLQILKDGQWIDVQPL NSIVINTGDQIEVLSNG+YKSVWHR+L 
Sbjct: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLHNSIVINTGDQIEVLSNGKYKSVWHRILA 240

Query: 241 SPNGNRRSLASFYNPSMEAIIAPAPKLVNKANQEVEKGYPKFVFGDYMSVYAEQKFLPKE 300
           +PNGNRRS+ASFYNPS+EA IAPA +LV+KANQEVE+GYPKFVFGDYMSVYAEQKFLPKE
Sbjct: 241 TPNGNRRSIASFYNPSLEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300

Query: 301 PRFQAVRAM 309
           PRFQAVRA+
Sbjct: 301 PRFQAVRAV 309

[Close]

BLAST of Cp4.1LG02g03090.1 vs. ExPASy TrEMBL
Match: A0A6J1IKI6 (1-aminocyclopropane-1-carboxylate oxidase 5-like OS=Cucurbita maxima OX=3661 GN=LOC111477086 PE=3 SV=1)

HSP 1 Score: 593 bits (1529), Expect = 1.32e-213
Identity = 284/309 (91.91%), Postives = 300/309 (97.09%), Query Frame = 0


Query: 1   MAIPVIDFSKLDGEERAKTLAQIANGCEEWGFFQLVNHGIPEKLLERVKKVCSECYKLER 60
           MAIPVIDFSKLDGEERAKTL QIANGCEEWGFFQLVNHGIPE+LLERVK+VCSECYKLER
Sbjct: 1   MAIPVIDFSKLDGEERAKTLNQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER 60

Query: 61  EENFKSSKPVKLLNDLLERKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL 120
           EE+F SSKPVKLLNDLLE KSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL
Sbjct: 61  EESFMSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL 120

Query: 121 KKLAAKVMAVMDENLGLPEGYIKAACNGGDGLDKAFFGTKVSHYPPCPHPELVNGLRAHT 180
           KKLA KVMA+MDENLGLPEGYIKAA NGG+GL+KAFFGTKVSHYPPCPHPELVNGLRAHT
Sbjct: 121 KKLAEKVMALMDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLP 240
           DAGGVILLFQDD+VGGLQILKDGQWIDVQPL NSIVINTGDQIEVLSNG+YKSVWHR+L 
Sbjct: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLHNSIVINTGDQIEVLSNGKYKSVWHRILA 240

Query: 241 SPNGNRRSLASFYNPSMEAIIAPAPKLVNKANQEVEKGYPKFVFGDYMSVYAEQKFLPKE 300
           +PNGNRRS+ASFYNPS+EA IAPA +LV+KANQEVE+GYPKFVFGDYMSVYAEQKFLPKE
Sbjct: 241 TPNGNRRSIASFYNPSLEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300

Query: 301 PRFQAVRAM 309
           PRFQAVRA+
Sbjct: 301 PRFQAVRAV 309

[Close]

BLAST of Cp4.1LG02g03090.1 vs. ExPASy TrEMBL
Match: A0A0A0L1U8 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G361270 PE=3 SV=1)

HSP 1 Score: 593 bits (1529), Expect = 1.32e-213
Identity = 284/309 (91.91%), Postives = 300/309 (97.09%), Query Frame = 0


Query: 1   MAIPVIDFSKLDGEERAKTLAQIANGCEEWGFFQLVNHGIPEKLLERVKKVCSECYKLER 60
           MAIPVIDFSKL+G+ERAKTLAQIANGCEEWGFFQLVNHGIPE+LLERVKKV SECYK+ER
Sbjct: 1   MAIPVIDFSKLNGQERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER 60

Query: 61  EENFKSSKPVKLLNDLLERKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL 120
           EE+FKSSKPVKLLNDLLE KSGEKLENLDWEDVFLLHD+NEWPSN+PGFKETM EYRSEL
Sbjct: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDDNEWPSNLPGFKETMREYRSEL 120

Query: 121 KKLAAKVMAVMDENLGLPEGYIKAACNGGDGLDKAFFGTKVSHYPPCPHPELVNGLRAHT 180
           +KLA KVMAVMDENLGLPEGYIKAA NGG+GL+KAFFGTK+SHYPPCPHPELVNGLRAHT
Sbjct: 121 RKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKISHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLP 240
           DAGGVILLFQDD+VGGLQILKDGQWIDVQP PNSIVINTGDQIEVLSNGRYKSVWHRVL 
Sbjct: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPFPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240

Query: 241 SPNGNRRSLASFYNPSMEAIIAPAPKLVNKANQEVEKGYPKFVFGDYMSVYAEQKFLPKE 300
           SPNGNRRS+ASFYNPSMEA IAPA +LV+KANQEVE+GYPKFVFGDYMSVYAEQKFLPKE
Sbjct: 241 SPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300

Query: 301 PRFQAVRAM 309
           PRFQAVRAM
Sbjct: 301 PRFQAVRAM 309

[Close]

BLAST of Cp4.1LG02g03090.1 vs. TAIR 10
Match: AT1G77330.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 476.9 bits (1226), Expect = 1.3e-134
Identity = 231/313 (73.80%), Postives = 263/313 (84.03%), Query Frame = 0


Query: 1   MAIPVIDFSKLDGEERAKTLAQIANGCEEWGFFQLVNHGIPEKLLERVKKVCSECYKLER 60
           MAIPVIDFSKL+GEER KTL++IA  CEEWGFFQLVNHGIP +LL +VKK+ S+CYK ER
Sbjct: 1   MAIPVIDFSKLNGEEREKTLSEIARACEEWGFFQLVNHGIPLELLNKVKKLSSDCYKTER 60

Query: 61  EENFKSSKPVKLLNDLLERKSGEKLENLDWEDVFLL--HDNNEWPSNIPGFKETMAEYRS 120
           EE FK+S PVKLLN+L+++ SGEKLEN+DWEDVF L  H+ NEWPSNI   KETM EYR 
Sbjct: 61  EEAFKTSNPVKLLNELVQKNSGEKLENVDWEDVFTLLDHNQNEWPSNI---KETMGEYRE 120

Query: 121 ELKKLAAKVMAVMDENLGLPEGYIKAACNGG--DGLDKAFFGTKVSHYPPCPHPELVNGL 180
           E++KLA+K+M VMDENLGLP+GYIK A N G  DG + AFFGTKVSHYPPCPHPELVNGL
Sbjct: 121 EVRKLASKMMEVMDENLGLPKGYIKKAFNEGMEDGEETAFFGTKVSHYPPCPHPELVNGL 180

Query: 181 RAHTDAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWH 240
           RAHTDAGGV+LLFQDDE  GLQ+LKDG+WIDVQPLPN+IVINTGDQIEVLSNGRYKS WH
Sbjct: 181 RAHTDAGGVVLLFQDDEYDGLQVLKDGEWIDVQPLPNAIVINTGDQIEVLSNGRYKSAWH 240

Query: 241 RVLPSPNGNRRSLASFYNPSMEAIIAPAPKLVNKANQEVEKGYPKFVFGDYMSVYAEQKF 300
           RVL    GNRRS+ASFYNPS +A I PA     + +   EK YPKFVFGDYM VYA QKF
Sbjct: 241 RVLAREEGNRRSIASFYNPSYKAAIGPAAVAEEEGS---EKKYPKFVFGDYMDVYANQKF 300

Query: 301 LPKEPRFQAVRAM 310
           +PKEPRF AV+++
Sbjct: 301 MPKEPRFLAVKSL 307

[Close]

BLAST of Cp4.1LG02g03090.1 vs. TAIR 10
Match: AT1G62380.1 (ACC oxidase 2 )

HSP 1 Score: 311.6 bits (797), Expect = 7.1e-85
Identity = 158/318 (49.69%), Postives = 212/318 (66.67%), Query Frame = 0


Query: 1   MAIPVIDFSKLDGEERAKTLAQIANGCEEWGFFQLVNHGIPEKLLERVKKVCSECYKLER 60
           M  PV+D SKL+GEER +T+A I   CE WGFF++VNHG+P  L+++++K+  + YK  +
Sbjct: 5   MKFPVVDLSKLNGEERDQTMALINEACENWGFFEIVNHGLPHDLMDKIEKMTKDHYKTCQ 64

Query: 61  EENFKSSKPVKLLNDLLERKSGEKL----ENLDWEDVFLLHDNNEWPSNIPGFKETMAEY 120
           E+ F         ND+L+ K  + L    E++DWE  F +    +  SN+    +   EY
Sbjct: 65  EQKF---------NDMLKSKGLDNLETEVEDVDWESTFYVRHLPQ--SNLNDISDVSDEY 124

Query: 121 RSELK-------KLAAKVMAVMDENLGLPEGYIKAACNGGDGLDKAFFGTKVSHYPPCPH 180
           R+ +K        LA  ++ ++ ENLGL +GY+K   +G  G     FGTKVS+YPPCP 
Sbjct: 125 RTAMKDFGKRLENLAEDLLDLLCENLGLEKGYLKKVFHGTKG---PTFGTKVSNYPPCPK 184

Query: 181 PELVNGLRAHTDAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNG 240
           PE++ GLRAHTDAGG+ILLFQDD+V GLQ+LKDG WIDV PL +SIVIN GDQ+EV++NG
Sbjct: 185 PEMIKGLRAHTDAGGIILLFQDDKVSGLQLLKDGDWIDVPPLNHSIVINLGDQLEVITNG 244

Query: 241 RYKSVWHRVLPSPNGNRRSLASFYNPSMEAIIAPAPKLVNKANQEVEKGYPKFVFGDYMS 300
           +YKSV HRV+    GNR S+ASFYNP  +A I+PA  LV K ++     YP FVF DYM 
Sbjct: 245 KYKSVLHRVVTQQEGNRMSVASFYNPGSDAEISPATSLVEKDSE-----YPSFVFDDYMK 303

Query: 301 VYAEQKFLPKEPRFQAVR 308
           +YA  KF PKEPRF A++
Sbjct: 305 LYAGVKFQPKEPRFAAMK 303

[Close]

BLAST of Cp4.1LG02g03090.1 vs. TAIR 10
Match: AT1G05010.1 (ethylene-forming enzyme )

HSP 1 Score: 311.2 bits (796), Expect = 9.3e-85
Identity = 153/318 (48.11%), Postives = 222/318 (69.81%), Query Frame = 0


Query: 2   AIPVIDFSKLDGEERAKTLAQIANGCEEWGFFQLVNHGIPEKLLERVKKVCSECYKLERE 61
           + P+I+  KL+GEERA T+ +I + CE WGFF+ VNHGI  +LL++V+K+  E YK   E
Sbjct: 3   SFPIINLEKLNGEERAITMEKIKDACENWGFFECVNHGISLELLDKVEKMTKEHYKKCME 62

Query: 62  ENFKSSKPVKLLNDLLERKSGEKLENLDWEDVFLL-----HDNNEWPSNIPGFKETMAEY 121
           E FK S   + L+ L       ++ ++DWE  F L      + ++ P     ++  M ++
Sbjct: 63  ERFKESIKNRGLDSL-----RSEVNDVDWESTFYLKHLPVSNISDVPDLDDDYRTLMKDF 122

Query: 122 RSELKKLAAKVMAVMDENLGLPEGYIKAACNGGDGLDKAFFGTKVSHYPPCPHPELVNGL 181
             +++KL+ +++ ++ ENLGL +GY+K    G     +  FGTKVS+YPPCP+P+LV GL
Sbjct: 123 AGKIEKLSEELLDLLCENLGLEKGYLKKVFYGS---KRPTFGTKVSNYPPCPNPDLVKGL 182

Query: 182 RAHTDAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWH 241
           RAHTDAGG+ILLFQDD+V GLQ+LKDG+W+DV P+ +SIV+N GDQ+EV++NG+YKSV H
Sbjct: 183 RAHTDAGGIILLFQDDKVSGLQLLKDGEWVDVPPVKHSIVVNLGDQLEVITNGKYKSVEH 242

Query: 242 RVLPSPNG-NRRSLASFYNPSMEAIIAPAPKLVNK-ANQEVEKGYPKFVFGDYMSVYAEQ 301
           RVL   +G  R S+ASFYNP  +++I PAP+L+ K A +E ++ YP+FVF DYM +Y+  
Sbjct: 243 RVLSQTDGEGRMSIASFYNPGSDSVIFPAPELIGKEAEKEKKENYPRFVFEDYMKLYSAV 302

Query: 302 KFLPKEPRFQAVRAMRGT 313
           KF  KEPRF+A++AM  T
Sbjct: 303 KFQAKEPRFEAMKAMETT 312

[Close]

BLAST of Cp4.1LG02g03090.1 vs. TAIR 10
Match: AT1G12010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 306.6 bits (784), Expect = 2.3e-83
Identity = 157/311 (50.48%), Postives = 210/311 (67.52%), Query Frame = 0


Query: 4   PVIDFSKLDGEERAKTLAQIANGCEEWGFFQLVNHGIPEKLLERVKKVCSECYKLEREEN 63
           PVID SKL+GEER +T+A I + C+ WGFF+LVNHG+P  L++ ++++  E YK   E+ 
Sbjct: 8   PVIDLSKLNGEERDQTMALIDDACQNWGFFELVNHGLPYDLMDNIERMTKEHYKKHMEQK 67

Query: 64  FKSSKPVKLLNDLLERKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSELKK- 123
           FK     K L D LE     ++E++DWE  F LH   +  SN+    +   EYR  +K  
Sbjct: 68  FKEMLRSKGL-DTLE----TEVEDVDWESTFYLHHLPQ--SNLYDIPDMSNEYRLAMKDF 127

Query: 124 ------LAAKVMAVMDENLGLPEGYIKAACNGGDGLDKAFFGTKVSHYPPCPHPELVNGL 183
                 LA +++ ++ ENLGL +GY+K   +G  G     F TK+S+YPPCP PE++ GL
Sbjct: 128 GKRLEILAEELLDLLCENLGLEKGYLKKVFHGTTG---PTFATKLSNYPPCPKPEMIKGL 187

Query: 184 RAHTDAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWH 243
           RAHTDAGG+ILLFQDD+V GLQ+LKDG W+DV PL +SIVIN GDQ+EV++NG+YKSV H
Sbjct: 188 RAHTDAGGLILLFQDDKVSGLQLLKDGDWVDVPPLKHSIVINLGDQLEVITNGKYKSVMH 247

Query: 244 RVLPSPNGNRRSLASFYNPSMEAIIAPAPKLVNKANQEVEKGYPKFVFGDYMSVYAEQKF 303
           RV+    GNR S+ASFYNP  +A I+PA  LV+K     +  YP FVF DYM +YA  KF
Sbjct: 248 RVMTQKEGNRMSIASFYNPGSDAEISPATSLVDK-----DSKYPSFVFDDYMKLYAGLKF 303

Query: 304 LPKEPRFQAVR 308
             KEPRF+A++
Sbjct: 308 QAKEPRFEAMK 303

[Close]

BLAST of Cp4.1LG02g03090.1 vs. TAIR 10
Match: AT2G19590.1 (ACC oxidase 1 )

HSP 1 Score: 270.8 bits (691), Expect = 1.4e-72
Identity = 144/316 (45.57%), Postives = 202/316 (63.92%), Query Frame = 0


Query: 1   MAIPVIDFSKLDGEERAKTLAQIANGCEEWGFFQLVNHGIPEKLLERVKKVCSECYKLER 60
           M IPVIDF++LDGE+R+KT++ + + C++WGFF + NHGI ++L+E+VKK+ +  Y+   
Sbjct: 9   MEIPVIDFAELDGEKRSKTMSLLDHACDKWGFFMVDNHGIDKELMEKVKKMINSHYEEHL 68

Query: 61  EENFKSSKPVKLLNDLLERKSGEKLENLDWEDVFLL-HDNNEWPSNIPGFKE----TMAE 120
           +E F  S+ VK L++        K  + DWE  F + H        IP   E    TM E
Sbjct: 69  KEKFYQSEMVKALSE-------GKTSDADWESSFFISHKPTSNICQIPNISEELSKTMDE 128

Query: 121 YRSELKKLAAKVMAVMDENLGLPEGYIKAACNGGDGLDKAFFGTKVSHYPPCPHPELVNG 180
           Y  +L K A ++  +M ENLGL +  I  A +G  G     FGTKV+ YP CP PEL+ G
Sbjct: 129 YVCQLHKFAERLSKLMCENLGLDQEDIMNAFSGPKG---PAFGTKVAKYPECPRPELMRG 188

Query: 181 LRAHTDAGGVILLFQDDEVGGLQILKDGQWIDVQPLP-NSIVINTGDQIEVLSNGRYKSV 240
           LR HTDAGG+ILL QDD+V GL+  KDG+W+ + P   N+I +NTGDQ+E+LSNGRYKSV
Sbjct: 189 LREHTDAGGIILLLQDDQVPGLEFFKDGKWVPIPPSKNNTIFVNTGDQLEILSNGRYKSV 248

Query: 241 WHRVLPSPNGNRRSLASFYNPSMEAIIAPAPKLVNKANQEVEKGYPK-FVFGDYMSVYAE 300
            HRV+   +G+R S+A+FYNP+ +AII+PAPKL+          YP  + F DY+ +Y+ 
Sbjct: 249 VHRVMTVKHGSRLSIATFYNPAGDAIISPAPKLL----------YPSGYRFQDYLKLYST 304

Query: 301 QKFLPKEPRFQAVRAM 310
            KF  K PR + ++ M
Sbjct: 309 TKFGDKGPRLETMKKM 304

The following BLAST results are available for this feature:

BLAST of Cp4.1LG02g03090.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0WPW4	1.8e-133	73.80	1-aminocyclopropane-1-carboxylate oxidase 5 OS=Arabidopsis thaliana OX=3702 GN=A...	[more]
Q9FR99	6.6e-128	71.70	1-aminocyclopropane-1-carboxylate oxidase OS=Musa acuminata OX=4641 GN=MAO1B PE=...	[more]
P31237	6.0e-89	51.12	1-aminocyclopropane-1-carboxylate oxidase OS=Actinidia deliciosa OX=3627 GN=ACO ...	[more]
Q00985	7.4e-87	50.00	1-aminocyclopropane-1-carboxylate oxidase 1 OS=Malus domestica OX=3750 PE=1 SV=1	[more]
Q08506	2.1e-86	50.48	1-aminocyclopropane-1-carboxylate oxidase 1 OS=Petunia hybrida OX=4102 GN=ACO1 P...	[more]

BLAST of Cp4.1LG02g03090.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023523759.1	8.01e-234	100.00	1-aminocyclopropane-1-carboxylate oxidase 5-like [Cucurbita pepo subsp. pepo]	[more]
XP_022941440.1	3.65e-230	98.40	1-aminocyclopropane-1-carboxylate oxidase 5-like [Cucurbita moschata]	[more]
KAG7031659.1	3.65e-230	98.40	1-aminocyclopropane-1-carboxylate oxidase 5, partial [Cucurbita argyrosperma sub...	[more]
XP_022981033.1	3.09e-222	95.51	1-aminocyclopropane-1-carboxylate oxidase 5-like [Cucurbita maxima]	[more]
XP_022936989.1	3.32e-214	92.23	1-aminocyclopropane-1-carboxylate oxidase 5-like isoform X1 [Cucurbita moschata]...	[more]

BLAST of Cp4.1LG02g03090.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FTN8	1.77e-230	98.40	1-aminocyclopropane-1-carboxylate oxidase 5-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1J108	1.50e-222	95.51	1-aminocyclopropane-1-carboxylate oxidase 5-like OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1FFA0	1.61e-214	92.23	1-aminocyclopropane-1-carboxylate oxidase 5-like isoform X1 OS=Cucurbita moschat...	[more]
A0A6J1IKI6	1.32e-213	91.91	1-aminocyclopropane-1-carboxylate oxidase 5-like OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A0A0L1U8	1.32e-213	91.91	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G...	[more]

BLAST of Cp4.1LG02g03090.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G77330.1	1.3e-134	73.80	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G62380.1	7.1e-85	49.69	ACC oxidase 2	[more]
AT1G05010.1	9.3e-85	48.11	ethylene-forming enzyme	[more]
AT1G12010.1	2.3e-83	50.48	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT2G19590.1	1.4e-72	45.57	ACC oxidase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 113..133
None	No IPR available	PANTHER	PTHR47991	OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE	coord: 2..307
None	No IPR available	PANTHER	PTHR47991:SF120	1-AMINOCYCLOPROPANE-1-CARBOXYLATE OXIDASE 5-LIKE	coord: 2..307
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 1..303
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 162..255 e-value: 8.5E-27 score: 93.6
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 3..99 e-value: 2.9E-19 score: 69.9
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 2..312 e-value: 4.8E-97 score: 327.1
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 151..255 score: 13.64346

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cp4.1LG02g03090	Cp4.1LG02g03090	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG02g03090.1:exon:003	Cp4.1LG02g03090.1:exon:003	exon
Cp4.1LG02g03090.1:exon:002	Cp4.1LG02g03090.1:exon:002	exon
Cp4.1LG02g03090.1:exon:001	Cp4.1LG02g03090.1:exon:001	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG02g03090.1:cds:003	Cp4.1LG02g03090.1:cds:003	CDS
Cp4.1LG02g03090.1:cds:002	Cp4.1LG02g03090.1:cds:002	CDS
Cp4.1LG02g03090.1:cds:001	Cp4.1LG02g03090.1:cds:001	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG02g03090.1	Cp4.1LG02g03090.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding