CmoCh18G011710.1 (mRNA) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh18G011710.1
Type	mRNA
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Ribonuclease T(2)
Location	Cmo_Chr18: 11834537 .. 11836147 (-)
Sequence length	801
RNA-Seq Expression	CmoCh18G011710.1
Synteny	CmoCh18G011710.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCTGTCATTTCCTGTATCCACAGATAATATCCCTAAACGATGCCTTCCATGTACGTCTCTATATAACACCCTTTCCTTTACTCCAAAGCTTATCAAACCAATACGCATCAGCCATGAGTTCCTTCACTCGCTTTGTTCTTATCAACCTTGTCATCTTCCAATGCCTGTCGCTTCTCTGCCTCGCCCAAGACTTTGATTTCTTCTACTTTGTTCAAAGTGTGAGTTTTTAAGTGTCATTTGAGACTAAACCCAGTTCGAAAGATTCAATTTTTAGTGTTGTTTTGTTGAGATTTTTGTGTTTCGTTTGGTTTTGCAGTGGCCTGGTTCTTACTGTGATACGGGAAGAAGCTGCTGCTATCCCCGCGCTGGAAAACCGTCTGCTGACTTCAAAATCCATGGCCTTTGGCCTAATTACAATGATGGCACTTACCCCTCTAATTGCGACTCATCCAACCCCTTCGATCGAACTCAGGTACTTATATGTGAGATCCCACATCGGTTGGAGAGGAAAACAAAGCATTCGTTATAAGGGTGTAGAAACCTCTCAAACGCGTTTTAAAACCTTGAGGAAAAGTCCAAAGAACTTGATAACTAATAGTGGTGGGCTTAGGCTGTTACAAATAGTATCAGAATCTGACGCCATGTAGTGTACAGACGCTGACCCCTAAGCGGGTGGATTGTGAGATCTCATATCAGTTGAAGAGAGGAGCGAAGCATTATTTATAAGGGTGTGGAAACCTCTCCGAGAAAAAGTTCAAATAAAACAATATCTGCTAACTGTGGACTGTTAAACAAATGGTAACAGAGCCAGACACCAGTCAGTGTTCTAGAAAGAACGCTGGCCCCCAAGAACATGATTGTGAGATACCACATCGTTTGGAGAGGGAACGAAGCATTCCTTATAAGGATGTGAAACCTCTTCCTATCAAACGAGTTTTAAAACATCAGAGTTAGACACTAAGCTGTGTGTCAGCGAAAACGCTGGCCCGTGGATTGTGAGATCCGACATTTGTTGGAGAAAGGAAACGAAACATTACTTAATAATAGTGTGAAAACCTCTCGACTCGTTTTAAAATTTTAATGAAAAGTACGAAAGAAAAAGCTTAAAGAACACAATATTTACTCGCGGTGGACTTTCTGTTACAACTTAATTCTTTTTAAGTGATGTTTTATATGGGACGCAGATATCAGATTTGGTGAGCAGCATGCAAAGGAACTGGCCATCACTAGCGTGTCCAAGCAGCGACAGCACGAAATTCTGGGCGCATGAGTGGGACAAACATGGGACATGCGCGGAGTCAGTGCTCGACCAACATGGATACTTTGAGACGAGCCTGAAGCTTAAAAGCCAAGCCAACATCCTGCAAGCTCTGGAAACAGCAGGAATAAACCCAGATGGAAGTTATTACAGTTTGGATAAGATTAAGAGTGCCATAGAAGAAGGAGTTGGGTACAGGCCTGGAATCACCTGCAACGTGGATGATTCCGGCAACCGTCAACTGTACGAGGTTTACTTGTGCGTGGATTCTTCCGCCTCTGGTTTCATAAATTGCCCAATTTTCCCGAGCGGACAATGTGGCTCTACCGTTGAGTTTCCCAAATTTTAG

mRNA sequence

ATGGCCTGTCATTTCCTGTATCCACAGATAATATCCCTAAACGATGCCTTCCATGTACGTCTCTATATAACACCCTTTCCTTTACTCCAAAGCTTATCAAACCAATACGCATCAGCCATGAGTTCCTTCACTCGCTTTGTTCTTATCAACCTTGTCATCTTCCAATGCCTGTCGCTTCTCTGCCTCGCCCAAGACTTTGATTTCTTCTACTTTGTTCAAAGTTGGCCTGGTTCTTACTGTGATACGGGAAGAAGCTGCTGCTATCCCCGCGCTGGAAAACCGTCTGCTGACTTCAAAATCCATGGCCTTTGGCCTAATTACAATGATGGCACTTACCCCTCTAATTGCGACTCATCCAACCCCTTCGATCGAACTCAGATATCAGATTTGGTGAGCAGCATGCAAAGGAACTGGCCATCACTAGCGTGTCCAAGCAGCGACAGCACGAAATTCTGGGCGCATGAGTGGGACAAACATGGGACATGCGCGGAGTCAGTGCTCGACCAACATGGATACTTTGAGACGAGCCTGAAGCTTAAAAGCCAAGCCAACATCCTGCAAGCTCTGGAAACAGCAGGAATAAACCCAGATGGAAGTTATTACAGTTTGGATAAGATTAAGAGTGCCATAGAAGAAGGAGTTGGGTACAGGCCTGGAATCACCTGCAACGTGGATGATTCCGGCAACCGTCAACTGTACGAGGTTTACTTGTGCGTGGATTCTTCCGCCTCTGGTTTCATAAATTGCCCAATTTTCCCGAGCGGACAATGTGGCTCTACCGTTGAGTTTCCCAAATTTTAG

Coding sequence (CDS)

ATGGCCTGTCATTTCCTGTATCCACAGATAATATCCCTAAACGATGCCTTCCATGTACGTCTCTATATAACACCCTTTCCTTTACTCCAAAGCTTATCAAACCAATACGCATCAGCCATGAGTTCCTTCACTCGCTTTGTTCTTATCAACCTTGTCATCTTCCAATGCCTGTCGCTTCTCTGCCTCGCCCAAGACTTTGATTTCTTCTACTTTGTTCAAAGTTGGCCTGGTTCTTACTGTGATACGGGAAGAAGCTGCTGCTATCCCCGCGCTGGAAAACCGTCTGCTGACTTCAAAATCCATGGCCTTTGGCCTAATTACAATGATGGCACTTACCCCTCTAATTGCGACTCATCCAACCCCTTCGATCGAACTCAGATATCAGATTTGGTGAGCAGCATGCAAAGGAACTGGCCATCACTAGCGTGTCCAAGCAGCGACAGCACGAAATTCTGGGCGCATGAGTGGGACAAACATGGGACATGCGCGGAGTCAGTGCTCGACCAACATGGATACTTTGAGACGAGCCTGAAGCTTAAAAGCCAAGCCAACATCCTGCAAGCTCTGGAAACAGCAGGAATAAACCCAGATGGAAGTTATTACAGTTTGGATAAGATTAAGAGTGCCATAGAAGAAGGAGTTGGGTACAGGCCTGGAATCACCTGCAACGTGGATGATTCCGGCAACCGTCAACTGTACGAGGTTTACTTGTGCGTGGATTCTTCCGCCTCTGGTTTCATAAATTGCCCAATTTTCCCGAGCGGACAATGTGGCTCTACCGTTGAGTTTCCCAAATTTTAG

Protein sequence

MACHFLYPQIISLNDAFHVRLYITPFPLLQSLSNQYASAMSSFTRFVLINLVIFQCLSLLCLAQDFDFFYFVQSWPGSYCDTGRSCCYPRAGKPSADFKIHGLWPNYNDGTYPSNCDSSNPFDRTQISDLVSSMQRNWPSLACPSSDSTKFWAHEWDKHGTCAESVLDQHGYFETSLKLKSQANILQALETAGINPDGSYYSLDKIKSAIEEGVGYRPGITCNVDDSGNRQLYEVYLCVDSSASGFINCPIFPSGQCGSTVEFPKF

Homology

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BLAST of CmoCh18G011710.1 vs. ExPASy Swiss-Prot
Match: P80022 (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 328.2 bits (840), Expect = 8.8e-89
Identity = 146/227 (64.32%), Postives = 182/227 (80.18%), Query Frame = 0


Query: 41  SSFTRFVLINLVIFQCLSLLCLAQDFDFFYFVQSWPGSYCDTGRSCCYPRAGKPSADFKI 100
           S+F+ F LI L+I QCLS+L  A+DFDFFYFVQ WPGSYCDT +SCCYP  GKP+ADF I
Sbjct: 5   SAFSLF-LILLIITQCLSVLNAAKDFDFFYFVQQWPGSYCDTKQSCCYPTTGKPAADFGI 64

Query: 101 HGLWPNYNDGTYPSNCDSSNPFDRTQISDLVSSMQRNWPSLACPSSDSTKFWAHEWDKHG 160
           HGLWPN NDGTYPSNCD ++P+D++QISDL+SSMQ+NWP+LACPS   + FW+HEW+KHG
Sbjct: 65  HGLWPNNNDGTYPSNCDPNSPYDQSQISDLISSMQQNWPTLACPSGSGSTFWSHEWEKHG 124

Query: 161 TCAESVL-DQHGYFETSLKLKSQANILQALETAGINPDGSYYSLDKIKSAIEEGVGYRPG 220
           TCAESVL +QH YF+ +L LK+Q ++L  L+ A I+PDG  Y L  I++AI+  +GY P 
Sbjct: 125 TCAESVLTNQHAYFKKALDLKNQIDLLSILQGADIHPDGESYDLVNIRNAIKSAIGYTPW 184

Query: 221 ITCNVDDSGNRQLYEVYLCVDSSASGFINCPIFPSGQCGSTVEFPKF 267
           I CNVD SGN QLY+VY+CVD S S  I CPIFP G+CG+++EFP F
Sbjct: 185 IQCNVDQSGNSQLYQVYICVDGSGSSLIECPIFPGGKCGTSIEFPTF 230

[Close]

BLAST of CmoCh18G011710.1 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 5.3e-86
Identity = 136/221 (61.54%), Postives = 171/221 (77.38%), Query Frame = 0


Query: 48  LINLVIF--QCLSLLCLAQDFDFFYFVQSWPGSYCDTGRSCCYPRAGKPSADFKIHGLWP 107
           LI+L++      S    ++DFDFFYFVQ WPGSYCDT + CCYP +GKP+ADF IHGLWP
Sbjct: 10  LISLLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGLWP 69

Query: 108 NYNDGTYPSNCDSSNPFDRTQISDLVSSMQRNWPSLACPSSDSTKFWAHEWDKHGTCAES 167
           NY DGTYPSNCD+S PFD + ISDL++SM+++WP+LACPS     FW HEW+KHGTC+ES
Sbjct: 70  NYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSES 129

Query: 168 VLDQHGYFETSLKLKSQANILQALETAGINPDGSYYSLDKIKSAIEEGVGYRPGITCNVD 227
           V+DQH YF+T+L LK + N+L AL  AGINPDG  YSL+ I+ +I+E +G+ P + CN D
Sbjct: 130 VIDQHEYFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECNRD 189

Query: 228 DSGNRQLYEVYLCVDSSASGFINCPIFPSGQCGSTVEFPKF 267
            SGN QLY+VYLCVD S SG I CP+FP G+CG+ +EFP F
Sbjct: 190 GSGNSQLYQVYLCVDRSGSGLIECPVFPHGKCGAEIEFPSF 230

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BLAST of CmoCh18G011710.1 vs. ExPASy Swiss-Prot
Match: P80196 (Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2)

HSP 1 Score: 316.6 bits (810), Expect = 2.6e-85
Identity = 141/223 (63.23%), Postives = 173/223 (77.58%), Query Frame = 0


Query: 47  VLINLVIFQCLSLLCL-AQDFDFFYFVQSWPGSYCDTGRSCCYPRAGKPSADFKIHGLWP 106
           +LI +++ QCL +LC+ +QDFDFFYFVQ WP SYCDT RSCCYP  GKP  DF IHGLWP
Sbjct: 8   LLIKIIVVQCLLVLCVTSQDFDFFYFVQQWPASYCDTRRSCCYPTTGKPDEDFSIHGLWP 67

Query: 107 NYNDGTYPSNCDSSNPFDRTQISDLVSSMQRNWPSLACPSSDSTKFWAHEWDKHGTCAES 166
           NY DG +P NCD  +  D ++ SDL+S+M++NWPSLACPSSD  KFW+HEW KHGTC  S
Sbjct: 68  NYKDGKWPQNCDRESSLDESEFSDLISTMEKNWPSLACPSSDGLKFWSHEWLKHGTC--S 127

Query: 167 VLDQHGYFETSLKLKSQANILQALETAGINP-DGSYYSLDKIKSAIEEGVGYRPGITCNV 226
            L+QH YF+T+L  K+++N+LQ L  AGI P +G YY ++ IK AIE+GVG+ P I CNV
Sbjct: 128 ALNQHAYFQTALDFKTKSNLLQNLNNAGIKPRNGDYYGVESIKKAIEKGVGHTPFIECNV 187

Query: 227 DDSGNRQLYEVYLCVDSSASGFINCPIFP-SGQCGSTVEFPKF 267
           D  GN QLY+VYLCVDSSAS FI+CPIFP  G+CGS +EFP F
Sbjct: 188 DSQGNHQLYQVYLCVDSSASKFIDCPIFPHGGKCGSKIEFPSF 228

[Close]

BLAST of CmoCh18G011710.1 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 5.2e-81
Identity = 130/218 (59.63%), Postives = 163/218 (74.77%), Query Frame = 0


Query: 49  INLVIFQCLSLLCLAQDFDFFYFVQSWPGSYCDTGRSCCYPRAGKPSADFKIHGLWPNYN 108
           I ++  Q L +   AQDFDFFYFV  WPG+YCD+  SCCYP+ GKP+ADF IHGLWPNY 
Sbjct: 5   IFILALQQLYVQSFAQDFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPNYK 64

Query: 109 DGTYPSNCDSSNPFDRTQISDLVSSMQRNWPSLACPSSDSTKFWAHEWDKHGTCAESVLD 168
            G +P NC+  + FD  ++SDL+S +QR WP+L+CPS+D  KFW HEW+KHGTCAES LD
Sbjct: 65  TGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESELD 124

Query: 169 QHGYFETSLKLKSQANILQALETAGINPDGSYYSLDKIKSAIEEGVGYRPGITCNVDDSG 228
           QH YFE  LKLK +AN+L AL  AGI PD  +Y +  I++ I++ VG+ PGI CN D S 
Sbjct: 125 QHDYFEAGLKLKQKANLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNKDSSH 184

Query: 229 NRQLYEVYLCVDSSASGFINCPIFPSGQCGSTVEFPKF 267
           N QLY++YLCVD+SAS FINCP+ P G+C S V+FPKF
Sbjct: 185 NSQLYQIYLCVDTSASKFINCPVMPHGRCDSRVQFPKF 222

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BLAST of CmoCh18G011710.1 vs. ExPASy Swiss-Prot
Match: Q7M438 (Ribonuclease DdI OS=Dictyostelium discoideum OX=44689 GN=ddiA PE=1 SV=3)

HSP 1 Score: 147.5 bits (371), Expect = 2.1e-34
Identity = 82/203 (40.39%), Postives = 114/203 (56.16%), Query Frame = 0


Query: 65  DFDFFYFVQSWPGSYCDTGRSCCYPRAGKPSADFKIHGLWPNYNDGTYPSNCDSSNPFDR 124
           DFDF+ FVQ W  SYCD+ ++C      K    F IHGLWP  +DG+YPS C S   F+ 
Sbjct: 31  DFDFYLFVQQWIYSYCDS-QTCI---QNKEREAFTIHGLWPENSDGSYPSFC-SGPSFNV 90

Query: 125 TQISDLVSSMQRNWPSLACPSSDSTKFWAHEWDKHGTCAES--VLDQHGYFETSLKLKSQ 184
             I DL   +  +WPSL  P++D   FW HE+ KHGTC+ +  + D H YF T +KL ++
Sbjct: 91  NAIQDLEDQLNFDWPSLTGPNTD---FWTHEFSKHGTCSITGPITDIHDYFATGIKLYTE 150

Query: 185 ANILQALETAGINP-DGSYYSLDKIKSAIEEGVGYRPGITCNVDDSGNRQLYEVYLCVDS 244
            NI  ALE+  I P D + Y    I +AI    G +PGI C+     + QL  V +C+D 
Sbjct: 151 FNITAALESENIYPSDSNTYKPVDITNAITTHFGGKPGIQCS-----SGQLSTVAVCIDK 210

Query: 245 SASGFINCPIFPSGQCGSTVEFP 265
           ++   ++CP      C  +V+FP
Sbjct: 211 NSLSIMDCPDLQGWSCSGSVKFP 220

[Close]

BLAST of CmoCh18G011710.1 vs. ExPASy TrEMBL
Match: A0A6J1G181 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111449750 PE=3 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 7.1e-134
Identity = 227/227 (100.00%), Postives = 227/227 (100.00%), Query Frame = 0


Query: 40  MSSFTRFVLINLVIFQCLSLLCLAQDFDFFYFVQSWPGSYCDTGRSCCYPRAGKPSADFK 99
           MSSFTRFVLINLVIFQCLSLLCLAQDFDFFYFVQSWPGSYCDTGRSCCYPRAGKPSADFK
Sbjct: 1   MSSFTRFVLINLVIFQCLSLLCLAQDFDFFYFVQSWPGSYCDTGRSCCYPRAGKPSADFK 60

Query: 100 IHGLWPNYNDGTYPSNCDSSNPFDRTQISDLVSSMQRNWPSLACPSSDSTKFWAHEWDKH 159
           IHGLWPNYNDGTYPSNCDSSNPFDRTQISDLVSSMQRNWPSLACPSSDSTKFWAHEWDKH
Sbjct: 61  IHGLWPNYNDGTYPSNCDSSNPFDRTQISDLVSSMQRNWPSLACPSSDSTKFWAHEWDKH 120

Query: 160 GTCAESVLDQHGYFETSLKLKSQANILQALETAGINPDGSYYSLDKIKSAIEEGVGYRPG 219
           GTCAESVLDQHGYFETSLKLKSQANILQALETAGINPDGSYYSLDKIKSAIEEGVGYRPG
Sbjct: 121 GTCAESVLDQHGYFETSLKLKSQANILQALETAGINPDGSYYSLDKIKSAIEEGVGYRPG 180

Query: 220 ITCNVDDSGNRQLYEVYLCVDSSASGFINCPIFPSGQCGSTVEFPKF 267
           ITCNVDDSGNRQLYEVYLCVDSSASGFINCPIFPSGQCGSTVEFPKF
Sbjct: 181 ITCNVDDSGNRQLYEVYLCVDSSASGFINCPIFPSGQCGSTVEFPKF 227

[Close]

BLAST of CmoCh18G011710.1 vs. ExPASy TrEMBL
Match: A0A6J1HX05 (Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111467429 PE=3 SV=1)

HSP 1 Score: 462.2 bits (1188), Expect = 1.4e-126
Identity = 216/227 (95.15%), Postives = 219/227 (96.48%), Query Frame = 0


Query: 40  MSSFTRFVLINLVIFQCLSLLCLAQDFDFFYFVQSWPGSYCDTGRSCCYPRAGKPSADFK 99
           MSS TRFVLI LVIFQCLSLLCLAQDFDFFYFVQSWPGSYCDTGRSCCYPRAGKPSADFK
Sbjct: 1   MSSCTRFVLITLVIFQCLSLLCLAQDFDFFYFVQSWPGSYCDTGRSCCYPRAGKPSADFK 60

Query: 100 IHGLWPNYNDGTYPSNCDSSNPFDRTQISDLVSSMQRNWPSLACPSSDSTKFWAHEWDKH 159
           IHGLWPNYND TYPSNCDSSNPFD TQISDLVSSMQRNWPSLACP SDSTKFWAHEW+KH
Sbjct: 61  IHGLWPNYNDATYPSNCDSSNPFDLTQISDLVSSMQRNWPSLACPGSDSTKFWAHEWNKH 120

Query: 160 GTCAESVLDQHGYFETSLKLKSQANILQALETAGINPDGSYYSLDKIKSAIEEGVGYRPG 219
           GTCAESVL+QHGYFETSL LKSQANILQALETAGINPDGSYYSLDKIKSAIE GVGY PG
Sbjct: 121 GTCAESVLNQHGYFETSLNLKSQANILQALETAGINPDGSYYSLDKIKSAIEGGVGYSPG 180

Query: 220 ITCNVDDSGNRQLYEVYLCVDSSASGFINCPIFPSGQCGSTVEFPKF 267
           ITCNVDDSGNRQLYEVYLCVDSSASGFINCPIFPSGQCGS+VEFPKF
Sbjct: 181 ITCNVDDSGNRQLYEVYLCVDSSASGFINCPIFPSGQCGSSVEFPKF 227

[Close]

BLAST of CmoCh18G011710.1 vs. ExPASy TrEMBL
Match: A0A0A0KQM0 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_5G605140 PE=3 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 9.1e-113
Identity = 188/224 (83.93%), Postives = 207/224 (92.41%), Query Frame = 0


Query: 44  TRFVLIN-LVIFQCLSLLCLAQDFDFFYFVQSWPGSYCDTGRSCCYPRAGKPSADFKIHG 103
           +RFVLIN +VIFQC SLL  AQDFDFFYFVQSWPGSYCDTGRSCCYP+ GKPS DFKIHG
Sbjct: 17  SRFVLINIIVIFQCFSLLSFAQDFDFFYFVQSWPGSYCDTGRSCCYPKTGKPSGDFKIHG 76

Query: 104 LWPNYNDGTYPSNCDSSNPFDRTQISDLVSSMQRNWPSLACPSSDSTKFWAHEWDKHGTC 163
           LWPNYNDGTYPSNCDSSNPFDRTQISDLVSSM++NWPSLACPSSD+TKFW+HEW+KHGTC
Sbjct: 77  LWPNYNDGTYPSNCDSSNPFDRTQISDLVSSMEKNWPSLACPSSDNTKFWSHEWNKHGTC 136

Query: 164 AESVLDQHGYFETSLKLKSQANILQALETAGINPDGSYYSLDKIKSAIEEGVGYRPGITC 223
           +ESVLDQH YFET+L LK QANILQAL+TAGINPDGSYYSLDKIKSAIEEG+   PGI+C
Sbjct: 137 SESVLDQHSYFETTLNLKQQANILQALQTAGINPDGSYYSLDKIKSAIEEGIKLSPGISC 196

Query: 224 NVDDSGNRQLYEVYLCVDSSASGFINCPIFPSGQCGSTVEFPKF 267
           NVD+SGN QLYE+YLCVDSSAS FI+CPIFP+  C S++EFPKF
Sbjct: 197 NVDESGNSQLYEIYLCVDSSASNFIDCPIFPNSNCASSLEFPKF 240

[Close]

BLAST of CmoCh18G011710.1 vs. ExPASy TrEMBL
Match: A0A6J1DBT7 (Ribonuclease T(2) OS=Momordica charantia OX=3673 GN=LOC111019062 PE=3 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 9.1e-113
Identity = 191/228 (83.77%), Postives = 208/228 (91.23%), Query Frame = 0


Query: 40  MSSFTRFVLINLVIFQCLSLLCLAQDFDFFYFVQSWPGSYCD-TGRSCCYPRAGKPSADF 99
           MS FTR +LI L+IFQCLSLLCLAQDFDFFYFVQSWP SYC+ +GRSCCYPR GKPSADF
Sbjct: 1   MSFFTRLLLIQLLIFQCLSLLCLAQDFDFFYFVQSWPASYCEASGRSCCYPRTGKPSADF 60

Query: 100 KIHGLWPNYNDGTYPSNCDSSNPFDRTQISDLVSSMQRNWPSLACPSSDSTKFWAHEWDK 159
           KIHGLWPNYNDGTYPSNCDSSNPFDRTQISDLV S Q+NWPSLACPSSDSTKFWAHEW+K
Sbjct: 61  KIHGLWPNYNDGTYPSNCDSSNPFDRTQISDLVGSTQKNWPSLACPSSDSTKFWAHEWNK 120

Query: 160 HGTCAESVLDQHGYFETSLKLKSQANILQALETAGINPDGSYYSLDKIKSAIEEGVGYRP 219
           HGTC+ESVLDQHGYFET+L LK+QANILQAL+TAGINPDG +YSL++IK AI++G+G  P
Sbjct: 121 HGTCSESVLDQHGYFETTLSLKNQANILQALQTAGINPDGRHYSLEEIKRAIKQGLGLAP 180

Query: 220 GITCNVDDSGNRQLYEVYLCVDSSASGFINCPIFPSGQCGSTVEFPKF 267
           GITCNVD SGNRQL EVYLCVDSSAS FI CPIFP G C STVEFP+F
Sbjct: 181 GITCNVDGSGNRQLNEVYLCVDSSASNFIECPIFPDGNCASTVEFPRF 228

[Close]

BLAST of CmoCh18G011710.1 vs. ExPASy TrEMBL
Match: A0A6J1H135 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111458606 PE=3 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 7.9e-109
Identity = 185/228 (81.14%), Postives = 204/228 (89.47%), Query Frame = 0


Query: 40  MSSFTRFVLINLVIFQCLSLLCL-AQDFDFFYFVQSWPGSYCDTGRSCCYPRAGKPSADF 99
           MS FTRF+LINL IFQCLSLLCL A+DFDFFYFVQSWPGSYCDT RSCCYP+AGKPS DF
Sbjct: 1   MSFFTRFLLINLAIFQCLSLLCLAAKDFDFFYFVQSWPGSYCDTRRSCCYPQAGKPSPDF 60

Query: 100 KIHGLWPNYNDGTYPSNCDSSNPFDRTQISDLVSSMQRNWPSLACPSSDSTKFWAHEWDK 159
           KIHGLWPNYNDGTYPSNCDSSN FD +Q+SDL  SMQR+WPSLACPSSDSTKFWAHEW+K
Sbjct: 61  KIHGLWPNYNDGTYPSNCDSSNTFDPSQVSDLEDSMQRDWPSLACPSSDSTKFWAHEWNK 120

Query: 160 HGTCAESVLDQHGYFETSLKLKSQANILQALETAGINPDGSYYSLDKIKSAIEEGVGYRP 219
           HGTCAESV+DQH YFE +L LK+QA+IL+AL+TAGINPDGS YSL KIKSAIE+GV   P
Sbjct: 121 HGTCAESVVDQHAYFEAALNLKTQADILKALQTAGINPDGSSYSLSKIKSAIEDGVKLSP 180

Query: 220 GITCNVDDSGNRQLYEVYLCVDSSASGFINCPIFPSGQCGSTVEFPKF 267
           GITCN D+SGN QLYEVY CVDSSAS FI+CP+FP G C S+VEFP+F
Sbjct: 181 GITCNTDESGNSQLYEVYFCVDSSASNFIDCPVFPDGNCNSSVEFPEF 228

[Close]

BLAST of CmoCh18G011710.1 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 318.9 bits (816), Expect = 3.8e-87
Identity = 136/221 (61.54%), Postives = 171/221 (77.38%), Query Frame = 0


Query: 48  LINLVIF--QCLSLLCLAQDFDFFYFVQSWPGSYCDTGRSCCYPRAGKPSADFKIHGLWP 107
           LI+L++      S    ++DFDFFYFVQ WPGSYCDT + CCYP +GKP+ADF IHGLWP
Sbjct: 10  LISLLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGLWP 69

Query: 108 NYNDGTYPSNCDSSNPFDRTQISDLVSSMQRNWPSLACPSSDSTKFWAHEWDKHGTCAES 167
           NY DGTYPSNCD+S PFD + ISDL++SM+++WP+LACPS     FW HEW+KHGTC+ES
Sbjct: 70  NYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSES 129

Query: 168 VLDQHGYFETSLKLKSQANILQALETAGINPDGSYYSLDKIKSAIEEGVGYRPGITCNVD 227
           V+DQH YF+T+L LK + N+L AL  AGINPDG  YSL+ I+ +I+E +G+ P + CN D
Sbjct: 130 VIDQHEYFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECNRD 189

Query: 228 DSGNRQLYEVYLCVDSSASGFINCPIFPSGQCGSTVEFPKF 267
            SGN QLY+VYLCVD S SG I CP+FP G+CG+ +EFP F
Sbjct: 190 GSGNSQLYQVYLCVDRSGSGLIECPVFPHGKCGAEIEFPSF 230

[Close]

BLAST of CmoCh18G011710.1 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 317.4 bits (812), Expect = 1.1e-86
Identity = 132/220 (60.00%), Postives = 176/220 (80.00%), Query Frame = 0


Query: 47  VLINLVIFQCLSLLCLAQDFDFFYFVQSWPGSYCDTGRSCCYPRAGKPSADFKIHGLWPN 106
           VL+ L++FQ L +    +DFDFFYFV  WPG+YCDT R+CCYP +GKP+ADF IHGLWPN
Sbjct: 9   VLLKLLVFQGLFVSRPQEDFDFFYFVLQWPGAYCDTSRACCYPTSGKPAADFGIHGLWPN 68

Query: 107 YNDGTYPSNCDSSNPFDRTQISDLVSSMQRNWPSLACPSSDSTKFWAHEWDKHGTCAESV 166
           YN G++PSNCD  + FDR+QISDLVSS+++NWP+L+CPS++   FW HEW+KHGTC+ESV
Sbjct: 69  YNGGSWPSNCDPDSQFDRSQISDLVSSLKKNWPTLSCPSNEGFNFWEHEWEKHGTCSESV 128

Query: 167 LDQHGYFETSLKLKSQANILQALETAGINPDGSYYSLDKIKSAIEEGVGYRPGITCNVDD 226
           +DQH YFE +LKLK +AN+LQ L+ +GINPD  +Y+LDKI +AI++G+G+ PGI CN D 
Sbjct: 129 MDQHEYFENALKLKQKANLLQILKNSGINPDDGFYNLDKITNAIKDGIGFTPGIECNKDP 188

Query: 227 SGNRQLYEVYLCVDSSASGFINCPIFPSGQCGSTVEFPKF 267
             N QL+++Y+CVD+S + FI CP+ P G C S ++F KF
Sbjct: 189 ERNAQLHQIYICVDTSGTEFIECPVLPRGSCPSQIQFSKF 228

[Close]

BLAST of CmoCh18G011710.1 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 302.4 bits (773), Expect = 3.7e-82
Identity = 130/218 (59.63%), Postives = 163/218 (74.77%), Query Frame = 0


Query: 49  INLVIFQCLSLLCLAQDFDFFYFVQSWPGSYCDTGRSCCYPRAGKPSADFKIHGLWPNYN 108
           I ++  Q L +   AQDFDFFYFV  WPG+YCD+  SCCYP+ GKP+ADF IHGLWPNY 
Sbjct: 5   IFILALQQLYVQSFAQDFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPNYK 64

Query: 109 DGTYPSNCDSSNPFDRTQISDLVSSMQRNWPSLACPSSDSTKFWAHEWDKHGTCAESVLD 168
            G +P NC+  + FD  ++SDL+S +QR WP+L+CPS+D  KFW HEW+KHGTCAES LD
Sbjct: 65  TGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESELD 124

Query: 169 QHGYFETSLKLKSQANILQALETAGINPDGSYYSLDKIKSAIEEGVGYRPGITCNVDDSG 228
           QH YFE  LKLK +AN+L AL  AGI PD  +Y +  I++ I++ VG+ PGI CN D S 
Sbjct: 125 QHDYFEAGLKLKQKANLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNKDSSH 184

Query: 229 NRQLYEVYLCVDSSASGFINCPIFPSGQCGSTVEFPKF 267
           N QLY++YLCVD+SAS FINCP+ P G+C S V+FPKF
Sbjct: 185 NSQLYQIYLCVDTSASKFINCPVMPHGRCDSRVQFPKF 222

[Close]

BLAST of CmoCh18G011710.1 vs. TAIR 10
Match: AT1G14210.1 (Ribonuclease T2 family protein )

HSP 1 Score: 238.4 bits (607), Expect = 6.5e-63
Identity = 104/223 (46.64%), Postives = 151/223 (67.71%), Query Frame = 0


Query: 47  VLINLVIFQCL--SLLCLAQDFDFFYFVQSWPGSYCDTGRSCCYPRAGKPSADFKIHGLW 106
           ++++L+I Q L  S      DF+FFY+V  WPG+ CD+ + CC P  G  ++DF IHGLW
Sbjct: 5   IIVSLLILQSLVVSSSQTEPDFNFFYWVNYWPGAICDSQKGCCPPTKGNTASDFIIHGLW 64

Query: 107 PNYNDGTYPSNCDSSNPFDRTQISDLVSSMQRNWPS---LACPSSDSTKFWAHEWDKHGT 166
           P +N+GT+P+ CD +N FD ++ISDLV  M++ W      ACPS++ T  W HEW+KHGT
Sbjct: 65  PQFNNGTWPAFCDQTNLFDISKISDLVCQMEKKWTEWGVWACPSNE-TNLWEHEWNKHGT 124

Query: 167 CAESVLDQHGYFETSLKLKSQANILQALETAGINPDGSYYSLDKIKSAIEEGVGYRPGIT 226
           C +S+ DQH YF T+LK K + ++L  L   GI P+  +YSLD+IK+AI+  +G+ PGI 
Sbjct: 125 CVQSIFDQHSYFRTNLKFKHKVHLLNILIQKGIKPNDGFYSLDEIKNAIKCAIGFAPGIE 184

Query: 227 CNVDDSGNRQLYEVYLCVDSSASGFINCPIFPSGQCGSTVEFP 265
           CN D  GN+QL+++Y+C+D+ A  F+ CP  P   C S ++FP
Sbjct: 185 CNEDVKGNKQLFQIYICLDNYAKEFVECPYVPDKSCASKIKFP 226

[Close]

BLAST of CmoCh18G011710.1 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 130.2 bits (326), Expect = 2.5e-30
Identity = 68/173 (39.31%), Postives = 95/173 (54.91%), Query Frame = 0


Query: 64  QDFDFFYFVQSWPGSYCDTGRSCCYPRAGKPSAD----FKIHGLWPNYNDGTYPSNCDSS 123
           ++FD+F     WPG+YC   R CC   A    +D    F IHGLWP+YNDG++PS C  S
Sbjct: 30  REFDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQFTIHGLWPDYNDGSWPSCCYRS 89

Query: 124 NPFDRTQISDLVSSMQRNWPSLACPSSDST-----KFWAHEWDKHGTCAESVL-DQHGYF 183
           + F   +IS L+  +++ WPSL+C S  S       FW HEW+KHGTC+  V  D++ YF
Sbjct: 90  D-FKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWGHEWEKHGTCSSPVFHDEYNYF 149

Query: 184 ETSLKLKSQANILQALETAG-INPDGSYYSLDKIKSAIEEGVGYRPGITCNVD 226
            T+L L  + N+   L  AG +  +   Y L  I +AI+      P + C  D
Sbjct: 150 LTTLNLYLKHNVTDVLYQAGYVASNSEKYPLGGIVTAIQNAFHITPEVVCKRD 201

The following BLAST results are available for this feature:

BLAST of CmoCh18G011710.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P80022	8.8e-89	64.32	Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2	[more]
P42813	5.3e-86	61.54	Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1	[more]
P80196	2.6e-85	63.23	Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2	[more]
P42815	5.2e-81	59.63	Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1	[more]
Q7M438	2.1e-34	40.39	Ribonuclease DdI OS=Dictyostelium discoideum OX=44689 GN=ddiA PE=1 SV=3	[more]

BLAST of CmoCh18G011710.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G181	7.1e-134	100.00	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111449750 PE=3 SV=1	[more]
A0A6J1HX05	1.4e-126	95.15	Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111467429 PE=3 SV=1	[more]
A0A0A0KQM0	9.1e-113	83.93	Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_5G605140 PE=3 SV=1	[more]
A0A6J1DBT7	9.1e-113	83.77	Ribonuclease T(2) OS=Momordica charantia OX=3673 GN=LOC111019062 PE=3 SV=1	[more]
A0A6J1H135	7.9e-109	81.14	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111458606 PE=3 SV=1	[more]

BLAST of CmoCh18G011710.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G02990.1	3.8e-87	61.54	ribonuclease 1	[more]
AT1G14220.1	1.1e-86	60.00	Ribonuclease T2 family protein	[more]
AT1G26820.1	3.7e-82	59.63	ribonuclease 3	[more]
AT1G14210.1	6.5e-63	46.64	Ribonuclease T2 family protein	[more]
AT2G39780.1	2.5e-30	39.31	ribonuclease 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 66..249 e-value: 3.3E-58 score: 196.9
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 45..266
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 60..266 e-value: 1.1E-78 score: 266.0
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 60..265
None	No IPR available	PANTHER	PTHR11240:SF64	RIBONUCLEASE 3-RELATED	coord: 45..266
IPR018188	Ribonuclease T2, His active site 1	PROSITE	PS00530	RNASE_T2_1	coord: 98..105
IPR033130	Ribonuclease T2, His active site 2	PROSITE	PS00531	RNASE_T2_2	coord: 151..162
IPR033697	Ribonuclease T2, eukaryotic	CDD	cd01061	RNase_T2_euk	coord: 66..260 e-value: 9.20899E-82 score: 242.238

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmoCh18G011710	CmoCh18G011710	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh18G011710.1:exon:4101	CmoCh18G011710.1:exon:4101	exon
CmoCh18G011710.1:exon:4102	CmoCh18G011710.1:exon:4102	exon
CmoCh18G011710.1:exon:4103	CmoCh18G011710.1:exon:4103	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh18G011710.1:cds	CmoCh18G011710.1:cds_3	CDS
CmoCh18G011710.1:cds	CmoCh18G011710.1:cds_2	CDS
CmoCh18G011710.1:cds	CmoCh18G011710.1:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmoCh18G011710.1	CmoCh18G011710.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006401	RNA catabolic process
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding