CmoCh16G009200.1 (mRNA) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh16G009200.1
Type	mRNA
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	gibberellin 2-beta-dioxygenase 8-like
Location	Cmo_Chr16: 5571826 .. 5576555 (-)
Sequence length	1248
RNA-Seq Expression	CmoCh16G009200.1
Synteny	CmoCh16G009200.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGGTGGGAAATATGAGTATGGAAATTGAACCGCCATTACATGTAAGGTACTATGCGCTGATGAAAGAGGAAAGTGAGAGTAAAAATGGGAGATGGGTAGGGGAAGAAGAAGAGAGGGAAGTAGTGGAGGAATGTGAGGAAGAAGAAGAGCTTCCAGTGATTGATTTAGGGCTTCTAAAAATGGGGAATTCAGAGAGGGAGAAGTGCATAAGTGAGATGGTTGAAGCTGCTAGGAATTGGGGTTTTTTCCAAGTGCTCAATCATGGGGTTCCAGAGAGGGTATTGAAGGGCATGATGTATGAGCAAAAAAAAGTGTTCAATCAACCATTTGCAAAGAAAAGTTTGAGCAATGTTCTTAATTTGGCTGGAACTTACCGATGGGGTAACCCAGTGGCCATGTCTCCAAGTCAAATCTCTTGGTCTGAAGCTTTTCATATGGCTGTTTTGGAGGTTTCAAACTTGGAGGAGCATGTCACCCTCAGGTATAACCACCCTAATTCTTTCAATTTTACCTTTCTACTTTCATTATGGATTCTTCTTATTCTTATTCTTCTTCTTCTTCTTCTTCTTCTTTTTCTTCTTCTTCTTCTTCTAAATTTTTTAATTTTCAAATCTTTTCTTGTTTGTATCATTAAAAACAAACGCATAATCAATCTAAGATTAGGGTTTTTCTTAATTAAGTGTGAAAATTAGCTTGGAAATCTCCTCCTCTTGTGCCACAAAGAAGAAGATTTAGTTTAGGCTTAATCATTAAGTTAAATTACACAATTAAGGGCTTTTAACTCTTTAATCCAAGTTCAAGTACAAGATTCTATGATATATATATATAACATAGAATAGTGCATTATGAAGAAGAAAAAATATGTGGCTAGAAGCACAAGATTGTGTAGTAATATAATATCAAGTTATTGGCATTATGAAGAAGTATGAAGTATGTGGGTACACACGATCAATTGGGAGAACAGCAGGTAACAATTTAGTCTATAAACTTTAAAAATGTAAGAATTTAGCGTTAATGCTTTAGATTTTGTATTGATTTAGAGAACACTCTGAAAAATATTGTTGAAATTAACCCATAAAGAGATTAGGGACCAAAATGTGTTGAAAATTATTTAAAAAATTTTGTTGTTCTCAAGAATACTCGAATGTTCGACAAAACAAGGATTAATGTAAACCCTAAAAAATGAGAATGTGTCCAATCTCAAAAGTAAGTATTAGAAAATGAATGCCTCAAAGTCTTTGGATGGCTTTTACTTGTGGTATATTATCAGAGAAGAGACTACTTTTACTTCAAAAAAAAACAAAAAAGTAAATAAATATCTGTGTGTTTGTCGTTGTTTTTCACCCTTTTCACTTGCTTCCCTGGTTTTGAATTCAATGTCTTCATGCAAGTACCAACACAAGAAAAGTACCTCCATACAGCTCATTTTGAATTACAAAAACATTAATTACAAAAGGTATTTTTTTTTATTTTTTTATTTTTATTTTTGGGTTTGAAAAGTTTTCATTATTGTTCTTGTCTTTTTATTCGATAAGTTTGAACTGATCTTTAAAAGTAATAGGTTTGAACTCTATTTAAGATAACAAATCAAAACACCTTGTACTAAAATAAATTTTCGTTTAAATATGTTTTAGTCTCTATACTTTCAATTTTTGTTTATCTTAGTGAACGTTCCACTTTGTTCATAAATCTTGTTCATAAATCTTCAACATGGATTTGTTTTTGTTTGTAGTTTCAAGATGTAAATTATAGGTCTGTATACTTTTAAAACTGACTATTTTCGTTCCTCAATTCAATCGAAATTTGAGCATGACAAGTTATTCACTATATTTGTTAAAATATAACTATAAATTGGTGATGTCTCACATTGGTTAGGAGGAGAACAAAACACTCTTTATAAGCGAGTAGAAACCTTACCCTAGGAGATGCGTTTTAAAGCCTTGAGAGGAAGCCCGAAAGGGAAAGCCCAAAGAGGACAATATTTGCTAGCGGTGGATCTGAGTTATTACAAATGGTATTAGAACCAGACACAGGACGATGTGTCAACCTTCTCGCTATTCCCCAAAGGGGGTAGACATGAGGCGGTGTGCCAGTATGGTCACTAGGCCCCGAAGGGGGGTGGATTGTGATGTCCCACATTGGTTGGGAGGAGAACAAAACACTCTTTATAAGCAAGTGGAAACCTTCCCATAGCGGATGCATTTTAAAAGCTTGAGGGGAACGGAAAAGGAAAGCCTAAAAATGACAATAAGGTGCTTACCAAATTTGTTGGTAATAAGTGATTTAGAGATTTACAAATTGGATGGTTTATTTAGAGTATAAAGACTAAAAAGGAACCAAATTTGAAATATTTGTATAGTACTTGTTTTTATCTCTCTTCAATTACGCACATGGGTAGAGGTTTATTGGATGGATGAGATGGGTCACTGTGGGGGGGTGAAGAAGCATATACACACATAGATAATATGTTTCTAGGGTACCCCACATGGGCTGAAAGGGCATGTGAAGTACCTGCGAGGGGTGCTTCCACAGCACCAGGGAGTGCATGGCTGAAAGGGCAATACTCATAGTTTGACATCACCCCATCACATGAACATCACATGAACACGTTTGTTAGCCCCGTGGGGGCACACCCTCCCTCCCTTATCTTATCTTCATTTTGGAGCTCAAAACATTCATTTTTTTGGCTCTTACTCCTCTTCTTTTCTTCCATTATTGCTCCTCAGATATGGTTTGTTTGGCAATAACATGACAAAAAAAAAAAAAAGAGCTCCCATTTTTCCTCATCTTTTTTTGTGCTTCACATTATTTGACAGACCCACAATGGAAGCAGTGGTGAAGAAGTTTGGGAGGTTAGCAGAGAGCATAGCGGAGATTTTAGGGCAAAGTTTGGGCATAAAAGCGTGCTATTTCAAGGAAAGGTGTGAGAAAGGAAGGAGCAGCTTTCGATTGAATAGATACCCACCTTGCCCATTTGCATCTCAAGTGTATGGCCTCATTCCTCACACAGATAGCGATTATCTCACAATACTCTACCAAGCTCAAGTCAGTGGGCTGCAGCTCATGAAAGATGGAAAATGGTTCGCTGTTAAACCAAACCCAAAAGCTTTGCTTGTCAACATCGGTGACTTATTTCAGGTCCTCACTTTTCTCTCTCTTTTCCCCTTTTTTTCATCTCAACTTTTCTGAGATTTTGGGTTTGGATGCTGTTCATAGATGGGAAATTAGAAGGTTTTTAGAGTGTTAAATATATATATATATAACATTAAATGGATCCATTCTTTCGGTTTAGTAGTAGTTTAATCGTTCGAAATGAATTCCATGGTTAGGTTTTTTTAAATATAACATTTGAATTAAAAATGTACTTATTGATTTCTGTTGATATCACAATATTTGAATTATACTCGAATTGATCGATAGCTCGAGTTGATTGATAGCTTTTAAGAGAGTTGTTGGGTTTTTAAAGCCAAAAGAACAGATGGTTAGGAAAACATGCTTGGTTTTAAGAAAGAAAAGACTATAAAGTAACAAAATCATTTAAAGATAAATTTGTAATGATTTTGAGCCCTATACTTATGTCTTGTTAGGGGATGTTTATTGCGTTAAAGAATCACGATCATTATCTATTTTGATTAAAAGTTCTCATGAATTTGTTTTTTGTTTCTTCAAAATGTCTAGTACTAATAGGATTGGTGTTCCTTACTTATAAACCTACGATTTTTCTCTTAATTAGTCAATATGAAACTAGTTCTCCCAATAATTTTCAGCCATCCCCCTTCCCCTGATATCTCTGGAGCTCTCGAACAATCTGTCTTTAATCGAGACTCAACTCTTTCTCTAGAGCCCTCAAACAAAATATATCATTTGTTTGCACTTGAGTCATTTTTTACTATACGTTCGAGACTCACAAATTCTTTGTTCGACATATGAGGATTCTATTGACATTGCTAAGTTAAGGGTATGACTCTGATACCATGTTAGGAACCACGACCATTCACAATAGCATGATATTATCCACTTTGAGCAAAAACTCTCCTTGCTTTGTTTTTAGTTTCCCCAAAAGGTTTCATACCCATGGAGATGATGTTCTTTACTTATATTCCCCCGGGAATCTCTACGTACTTGTCCAGTCTAGAACAAGATCTTCCATACGTTTCATATATGATTTCAATCAACCAAATTGTAAGCTTATAAGATCTAGAATTTGAACATTGGTTCCTCTCAAGAATATAGGAAAAGAAAGAACATAAACATAGCACCAACCTTTCTTAGGAGAGTCATGTTTGCTGAAAATGTGATGCAGGTTGTGAGCAATGATGCATTTAGGAGCCTCAAACACAGAGTGGTTGCTTCTGAAGCAGTGGAAAGATTTTCATTCGCATATTTCTATAGCCCATCTGATGATGTGATGATTGAGAGTTGCACAAAGCCAAGCATTTACAGGCAATTCAGCTACAGAGAGTACAGACAACAAATAGAAAAAGATGTAGAGAAAACAGGCAACAAAGTAGGGCTTCCAAGGTTCTTCTTGCACAACATGTTGCCTCATTTGGGTCAATGACTCAAGTGTCTAATGTCTATTCTAGGGGCTCTACCTAGCCATCATACAAAAATAATCCAAGTAGGAGGCTTAGATTTGGGTGTGTTTTGTATCAAATATATCGTTTCAATATTATAATGTTGTTGTCTACTTAAAAATCTCGGTCCATTAATATAAGCAGATATATAC

mRNA sequence

GAGGTGGGAAATATGAGTATGGAAATTGAACCGCCATTACATGTAAGGTACTATGCGCTGATGAAAGAGGAAAGTGAGAGTAAAAATGGGAGATGGGTAGGGGAAGAAGAAGAGAGGGAAGTAGTGGAGGAATGTGAGGAAGAAGAAGAGCTTCCAGTGATTGATTTAGGGCTTCTAAAAATGGGGAATTCAGAGAGGGAGAAGTGCATAAGTGAGATGGTTGAAGCTGCTAGGAATTGGGGTTTTTTCCAAGTGCTCAATCATGGGGTTCCAGAGAGGGTATTGAAGGGCATGATGTATGAGCAAAAAAAAGTGTTCAATCAACCATTTGCAAAGAAAAGTTTGAGCAATGTTCTTAATTTGGCTGGAACTTACCGATGGGGTAACCCAGTGGCCATGTCTCCAAGTCAAATCTCTTGGTCTGAAGCTTTTCATATGGCTGTTTTGGAGGTTTCAAACTTGGAGGAGCATGTCACCCTCAGTTTCAAGATATATGGTTTACCCACAATGGAAGCAGTGGTGAAGAAGTTTGGGAGGTTAGCAGAGAGCATAGCGGAGATTTTAGGGCAAAGTTTGGGCATAAAAGCGTGCTATTTCAAGGAAAGGTGTGAGAAAGGAAGGAGCAGCTTTCGATTGAATAGATACCCACCTTGCCCATTTGCATCTCAAGTGTATGGCCTCATTCCTCACACAGATAGCGATTATCTCACAATACTCTACCAAGCTCAAGTCAGTGGGCTGCAGCTCATGAAAGATGGAAAATGGTTCGCTGTTAAACCAAACCCAAAAGCTTTGCTTGTCAACATCGGTGACTTATTTCAGGTTGTGAGCAATGATGCATTTAGGAGCCTCAAACACAGAGTGGTTGCTTCTGAAGCAGTGGAAAGATTTTCATTCGCATATTTCTATAGCCCATCTGATGATGTGATGATTGAGAGTTGCACAAAGCCAAGCATTTACAGGCAATTCAGCTACAGAGAGTACAGACAACAAATAGAAAAAGATGTAGAGAAAACAGGCAACAAAGTAGGGCTTCCAAGGTTCTTCTTGCACAACATGTTGCCTCATTTGGGTCAATGACTCAAGTGTCTAATGTCTATTCTAGGGGCTCTACCTAGCCATCATACAAAAATAATCCAAGTAGGAGGCTTAGATTTGGGTGTGTTTTGTATCAAATATATCGTTTCAATATTATAATGTTGTTGTCTACTTAAAAATCTCGGTCCATTAATATAAGCAGATATATAC

Coding sequence (CDS)

ATGAGTATGGAAATTGAACCGCCATTACATGTAAGGTACTATGCGCTGATGAAAGAGGAAAGTGAGAGTAAAAATGGGAGATGGGTAGGGGAAGAAGAAGAGAGGGAAGTAGTGGAGGAATGTGAGGAAGAAGAAGAGCTTCCAGTGATTGATTTAGGGCTTCTAAAAATGGGGAATTCAGAGAGGGAGAAGTGCATAAGTGAGATGGTTGAAGCTGCTAGGAATTGGGGTTTTTTCCAAGTGCTCAATCATGGGGTTCCAGAGAGGGTATTGAAGGGCATGATGTATGAGCAAAAAAAAGTGTTCAATCAACCATTTGCAAAGAAAAGTTTGAGCAATGTTCTTAATTTGGCTGGAACTTACCGATGGGGTAACCCAGTGGCCATGTCTCCAAGTCAAATCTCTTGGTCTGAAGCTTTTCATATGGCTGTTTTGGAGGTTTCAAACTTGGAGGAGCATGTCACCCTCAGTTTCAAGATATATGGTTTACCCACAATGGAAGCAGTGGTGAAGAAGTTTGGGAGGTTAGCAGAGAGCATAGCGGAGATTTTAGGGCAAAGTTTGGGCATAAAAGCGTGCTATTTCAAGGAAAGGTGTGAGAAAGGAAGGAGCAGCTTTCGATTGAATAGATACCCACCTTGCCCATTTGCATCTCAAGTGTATGGCCTCATTCCTCACACAGATAGCGATTATCTCACAATACTCTACCAAGCTCAAGTCAGTGGGCTGCAGCTCATGAAAGATGGAAAATGGTTCGCTGTTAAACCAAACCCAAAAGCTTTGCTTGTCAACATCGGTGACTTATTTCAGGTTGTGAGCAATGATGCATTTAGGAGCCTCAAACACAGAGTGGTTGCTTCTGAAGCAGTGGAAAGATTTTCATTCGCATATTTCTATAGCCCATCTGATGATGTGATGATTGAGAGTTGCACAAAGCCAAGCATTTACAGGCAATTCAGCTACAGAGAGTACAGACAACAAATAGAAAAAGATGTAGAGAAAACAGGCAACAAAGTAGGGCTTCCAAGGTTCTTCTTGCACAACATGTTGCCTCATTTGGGTCAATGA

Protein sequence

MSMEIEPPLHVRYYALMKEESESKNGRWVGEEEEREVVEECEEEEELPVIDLGLLKMGNSEREKCISEMVEAARNWGFFQVLNHGVPERVLKGMMYEQKKVFNQPFAKKSLSNVLNLAGTYRWGNPVAMSPSQISWSEAFHMAVLEVSNLEEHVTLSFKIYGLPTMEAVVKKFGRLAESIAEILGQSLGIKACYFKERCEKGRSSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQAQVSGLQLMKDGKWFAVKPNPKALLVNIGDLFQVVSNDAFRSLKHRVVASEAVERFSFAYFYSPSDDVMIESCTKPSIYRQFSYREYRQQIEKDVEKTGNKVGLPRFFLHNMLPHLGQ

Homology

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BLAST of CmoCh16G009200.1 vs. ExPASy Swiss-Prot
Match: O49561 (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 322.0 bits (824), Expect = 8.4e-87
Identity = 170/344 (49.42%), Postives = 224/344 (65.12%), Query Frame = 0


Query: 5   IEPPLHVRYYALMKEESESKNGRWVGEEEEREVVEECEEEEELPVIDLG-LLKMGNSERE 64
           ++PP +  Y  L+  +   K+   V E      V    EE ELPVID+  L+     ERE
Sbjct: 1   MDPPFNEIYNNLLYNQITKKDND-VSEIPFSFSVTAVVEEVELPVIDVSRLIDGAEEERE 60

Query: 65  KCISEMVEAARNWGFFQVLNHGVPERVLKGMMYEQKKVFNQPFAKKSLSNVLNLAGTYRW 124
           KC   +  A+R WGFFQV+NHG+   VL+ M  EQ +VF +PF KKS S   + AG+YRW
Sbjct: 61  KCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFREPFDKKSKSEKFS-AGSYRW 120

Query: 125 GNPVAMSPSQISWSEAFHMAVLEVSNLEEHVTLSFKIYGLPTMEAVVKKFGRLAESIAEI 184
           G P A S  Q+SWSEAFH+ + ++S+ ++  TLS       TME    +   LA  +AE+
Sbjct: 121 GTPSATSIRQLSWSEAFHVPMTDISDNKDFTTLS------STMEKFASESEALAYMLAEV 180

Query: 185 LGQSLGIKACYFKERCEKGRSSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQAQVSGL 244
           L +  G  + +FKE C +     R+NRYPPCP  S+VYGL+PHTDSD+LTILYQ QV GL
Sbjct: 181 LAEKSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVYGLMPHTDSDFLTILYQDQVGGL 240

Query: 245 QLMKDGKWFAVKPNPKALLVNIGDLFQVVSNDAFRSLKHRVVASEAVERFSFAYFYSPSD 304
           QL+KD +W AVKPNPKAL++NIGDLFQ  SN  ++S++HRV+ +  VERFS AYF  PS 
Sbjct: 241 QLIKDNRWIAVKPNPKALIINIGDLFQAWSNGMYKSVEHRVMTNPKVERFSTAYFMCPSY 300

Query: 305 DVMIESCTKPSIYRQFSYREYRQQIEKDVEKTGNKVGLPRFFLH 348
           D +IE  +    YR FS+RE+RQQ+++DV+K G KVGLPRF  H
Sbjct: 301 DAVIECSSDRPAYRNFSFREFRQQVQEDVKKFGFKVGLPRFLNH 336

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BLAST of CmoCh16G009200.1 vs. ExPASy Swiss-Prot
Match: Q7XP65 (Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX6 PE=1 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 9.6e-75
Identity = 139/313 (44.41%), Postives = 198/313 (63.26%), Query Frame = 0


Query: 44  EEELPVIDLGLLKMGNS-EREKCISEMVEAARNWGFFQVLNHGVPERVLKGMMYEQKKVF 103
           E +LP++DL  L  G + ER+ C   M  AA  WGFFQ+ NHGV   +++ M  EQ ++F
Sbjct: 47  ERDLPMVDLERLTRGGAGERKACAGAMARAASEWGFFQLTNHGVGRELMEEMRREQARLF 106

Query: 104 NQPFAKKSLSNVLNLAGTYRWGNPVAMSPSQISWSEAFHMAVLEVSNLEEHVTLSFKIYG 163
             PF  K  + +LN  G+YRWGNP A S   +SWSEAFH+ +  +S  +        + G
Sbjct: 107 RLPFETKEKAGLLN--GSYRWGNPTATSLRHLSWSEAFHVPLASISGADCDFGDLTSLRG 166

Query: 164 LPTMEAVVKKFGRLAESIAEILGQSL-------GIKACYFKERCEKGRSSFRLNRYPPCP 223
           +  M+ V +   R+A ++A  L + L          A +F   C++     RLNRYP CP
Sbjct: 167 V--MQEVAEAMSRVANTVAAALAEELTGRGGGGASAAPWFPAGCDETTCFLRLNRYPACP 226

Query: 224 FASQVYGLIPHTDSDYLTILYQAQVSGLQLMKDGKWFAVKPNPKALLVNIGDLFQVVSND 283
           FA+  +GL+PHTDSD+LT+L Q QV GL LMKD +W AV+P P AL+VNIGDLFQ  SN+
Sbjct: 227 FAADTFGLVPHTDSDFLTVLCQDQVGGLHLMKDSRWVAVRPRPDALVVNIGDLFQAWSNN 286

Query: 284 AFRSLKHRVVASEAVERFSFAYFYSPSDDVMIESCTKPSIYRQFSYREYRQQIEKDVEKT 343
            ++S++H+VVA+   +R S AYF  PS D ++ +C +PS YR F++ EYR+++++DV  T
Sbjct: 287 RYKSVEHKVVANAKTDRLSVAYFLCPSYDSLVGTCGEPSPYRAFTFGEYRKKVQEDVRTT 346

Query: 344 GNKVGLPRFFLHN 349
           G K+GLP FF H+
Sbjct: 347 GKKIGLPNFFKHS 355

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BLAST of CmoCh16G009200.1 vs. ExPASy Swiss-Prot
Match: Q9C6I4 (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 3.1e-73
Identity = 137/303 (45.21%), Postives = 204/303 (67.33%), Query Frame = 0


Query: 46  ELPVIDLGLLKMGNS-EREKCISEMVEAARNWGFFQVLNHGVPERVLKGMMYEQKKVFNQ 105
           +LPVIDL  L  G   +R++C+ +MV AA+ WGFFQ++NHG+P+ V + M+ E+KK+F+Q
Sbjct: 39  KLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQ 98

Query: 106 PFAKKSLSNVLNLA-GTYRWGNPVAMSPSQISWSEAFHMAVLEVSNLEEHVTLSFKIYGL 165
           PF+ K      +L+  +YRWGNP A SP+Q S SEAFH+ + EVS + +       I   
Sbjct: 99  PFSVKVRERFSDLSKNSYRWGNPSATSPAQYSVSEAFHIILSEVSRISDDRNNLRTI--- 158

Query: 166 PTMEAVVKKFGRLAESIAEILGQSLGIKACYFKERCEKGRSSFRLNRYPPCPFASQVYGL 225
             +E  V++  R+A+ I EILG+ + + + YF+   E   S  RLN+Y P  F S+V+GL
Sbjct: 159 --VETYVQEIARVAQMICEILGKQVNVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGL 218

Query: 226 IPHTDSDYLTILYQAQVSGLQLMKDGKWFAVKPNPKALLVNIGDLFQVVSNDAFRSLKHR 285
           +PHTD+ +LTIL Q Q+ GL+L  +G+W +VKP  +AL VNIGD+FQ +SN  ++S++HR
Sbjct: 219 VPHTDTSFLTILSQDQIGGLELENNGQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHR 278

Query: 286 VVASEAVERFSFAYFYSPSDDVMIESCTKPSIYRQFSYREYRQQIEKDVEKTGNKVGLPR 345
           V++   +ER S A+F  P  +  I+    P  YR+FS+REY++Q E DV++TG+KVGL R
Sbjct: 279 VISPANIERMSIAFFVCPYLETEIDCFGYPKKYRRFSFREYKEQSEHDVKETGDKVGLSR 336

Query: 346 FFL 347
           F +
Sbjct: 339 FLI 336

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BLAST of CmoCh16G009200.1 vs. ExPASy Swiss-Prot
Match: Q8LGZ9 (Gibberellin 2-beta-dioxygenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX5 PE=1 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 7.9e-61
Identity = 137/317 (43.22%), Postives = 186/317 (58.68%), Query Frame = 0


Query: 38  VEECEEEEELPVIDL-GLLKMGNSEREKCISEMVEAARNWGFFQVLNHGVPERVLKGMMY 97
           V+EC    ELPVIDL GL++      + C ++MV AA  WGFFQV NHGVP+ +L+ +  
Sbjct: 37  VDEC----ELPVIDLAGLME----AEQVCRADMVRAASEWGFFQVTNHGVPQALLRELHD 96

Query: 98  EQKKVFNQPFAKKSLSNVLNLA-GTYRWGNPVAMSPSQISWSEAFHMAVLEVSNLEEHVT 157
            Q  VF +PF +K    +L  +  +YRWG P A    Q+SWSEA+H+ +           
Sbjct: 97  AQVAVFRRPFQEKVTERLLGFSPESYRWGTPTAKCLEQLSWSEAYHIPMTTPRP------ 156

Query: 158 LSFKIYGLPTMEAVVKKFGRLAESIAEILGQSL-GIKACYFKERCEKGRSSFRLNRYPPC 217
            S  I     +E V +    LA+ +AEIL + L G           +     RLNRYPPC
Sbjct: 157 -STSIRARAVIEEVSRAMYELAQKLAEILMRGLPGAGEGETMVTTREETCFLRLNRYPPC 216

Query: 218 PFASQVYGLIPHTDSDYLTILYQAQ--VSGLQLMKDGKWFAVKPNPKALLVNIGDLFQVV 277
             A   +GL PHTDSD LTI++Q Q  V GLQL+K G+W AVKP+P  L+VN+GDL Q  
Sbjct: 217 AMAMGGFGLCPHTDSDLLTIVHQQQDTVGGLQLLKGGRWVAVKPSPSTLIVNVGDLLQAW 276

Query: 278 SNDAFRSLKHRVVASEAVERFSFAYFYSPSDDVMIESCTK-----PSIYRQFSYREYRQQ 337
           SND ++S++HRV+A+  +ERFS A+F  PS   +I   +       + YR F++ EYR+Q
Sbjct: 277 SNDVYKSVEHRVMANATLERFSMAFFLCPSYHTLIIPSSSHVHDDDAHYRSFTFGEYRKQ 336

Query: 338 IEKDVEKTGNKVGLPRF 345
           I +DV  TG K+GL RF
Sbjct: 337 IMEDVRSTGRKIGLHRF 338

[Close]

BLAST of CmoCh16G009200.1 vs. ExPASy Swiss-Prot
Match: Q9ZSA7 (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 2.7e-37
Identity = 95/292 (32.53%), Postives = 143/292 (48.97%), Query Frame = 0


Query: 45  EELPVIDLGLLKMGNSEREKCISEMVEAARNWGFFQVLNHGVPERVLKGMMYEQKKVFNQ 104
           + +P+IDL  L   N  R   I++   A  + GFFQ+ NHGVPE  +K MM   ++ F Q
Sbjct: 40  DSIPLIDLHDLHGPN--RADIINQFAHACSSCGFFQIKNHGVPEETIKKMMNAAREFFRQ 99

Query: 105 PFAKKSLSNVLNLAGTYRWGNPVAMSPSQIS-WSEAFHM---AVLEVSNLEEHVTLSFKI 164
             +++      +   T R      +S  ++S W +   +    + +  N      +SF+ 
Sbjct: 100 SESERVKHYSADTKKTTRLSTSFNVSKEKVSNWRDFLRLHCYPIEDFINEWPSTPISFR- 159

Query: 165 YGLPTMEAVVKKFGRLAESIAEILGQSLGIKACYFKERCEKGRSSFRLNRYPPCPFASQV 224
                          L  ++ E + +SLG+          K      +N YP CP     
Sbjct: 160 ---EVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIGKHGQHMAINYYPRCPQPELT 219

Query: 225 YGLIPHTDSDYLTILYQAQVSGLQLMKDGKWFAVKPNPKALLVNIGDLFQVVSNDAFRSL 284
           YGL  H D++ +T+L Q +VSGLQ+ KDGKW AV P P   +VN+GD  QV+SN+ ++S+
Sbjct: 220 YGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNTFIVNLGDQMQVISNEKYKSV 279

Query: 285 KHRVVASEAVERFSFAYFYSPSDDVMI--------ESCTKPSIYRQFSYREY 325
            HR V +  +ER S   FY PS+D +I        E    P+IYR F+Y EY
Sbjct: 280 LHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDSPAIYRNFTYAEY 325

[Close]

BLAST of CmoCh16G009200.1 vs. ExPASy TrEMBL
Match: A0A6J1F0S8 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita moschata OX=3662 GN=LOC111438457 PE=3 SV=1)

HSP 1 Score: 699.5 bits (1804), Expect = 7.1e-198
Identity = 348/355 (98.03%), Postives = 348/355 (98.03%), Query Frame = 0


Query: 1   MSMEIEPPLHVRYYALMKEESESKNGRWVGEEEEREVVEECEEEEELPVIDLGLLKMGNS 60
           MSMEIEPPLHVRYYALMKEESESKNGRWVGEEEEREVVEECEEEEELPVIDLGLLKMGNS
Sbjct: 1   MSMEIEPPLHVRYYALMKEESESKNGRWVGEEEEREVVEECEEEEELPVIDLGLLKMGNS 60

Query: 61  EREKCISEMVEAARNWGFFQVLNHGVPERVLKGMMYEQKKVFNQPFAKKSLSNVLNLAGT 120
           EREKCISEMVEAARNWGFFQVLNHGVPERVLKGMMYEQKKVFNQPFAKKSLSNVLNLAGT
Sbjct: 61  EREKCISEMVEAARNWGFFQVLNHGVPERVLKGMMYEQKKVFNQPFAKKSLSNVLNLAGT 120

Query: 121 YRWGNPVAMSPSQISWSEAFHMAVLEVSNLEEHVTLSFKIYGLPTMEAVVKKFGRLAESI 180
           YRWGNPVAMSPSQISWSEAFHMAVLEVSNLEEHVTL       PTMEAVVKKFGRLAESI
Sbjct: 121 YRWGNPVAMSPSQISWSEAFHMAVLEVSNLEEHVTLR------PTMEAVVKKFGRLAESI 180

Query: 181 AEILGQSLGIKACYFKERCEKGRSSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQAQV 240
           AEILGQSLGIKACYFKERCEKGRSSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQAQV
Sbjct: 181 AEILGQSLGIKACYFKERCEKGRSSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQAQV 240

Query: 241 SGLQLMKDGKWFAVKPNPKALLVNIGDLFQVVSNDAFRSLKHRVVASEAVERFSFAYFYS 300
           SGLQLMKDGKWFAVKPNPKALLVNIGDLFQVVSNDAFRSLKHRVVASEAVERFSFAYFYS
Sbjct: 241 SGLQLMKDGKWFAVKPNPKALLVNIGDLFQVVSNDAFRSLKHRVVASEAVERFSFAYFYS 300

Query: 301 PSDDVMIESCTKPSIYRQFSYREYRQQIEKDVEKTGNKVGLPRFFLHNMLPHLGQ 356
           PSDDVMIESCTKPSIYRQFSYREYRQQIEKDVEKTGNKVGLPRFFLHNMLPHLGQ
Sbjct: 301 PSDDVMIESCTKPSIYRQFSYREYRQQIEKDVEKTGNKVGLPRFFLHNMLPHLGQ 349

[Close]

BLAST of CmoCh16G009200.1 vs. ExPASy TrEMBL
Match: A0A6J1JAT6 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita maxima OX=3661 GN=LOC111483296 PE=3 SV=1)

HSP 1 Score: 674.9 bits (1740), Expect = 1.9e-190
Identity = 337/355 (94.93%), Postives = 342/355 (96.34%), Query Frame = 0


Query: 1   MSMEIEPPLHVRYYALMKEESESKNGRWVGEEEEREVVEECEEEEELPVIDLGLLKMGNS 60
           MSMEIEPPL VRYYALMKEESESKNGRWVGEEEEREVVEECEEEEELPVIDLGLLKMGNS
Sbjct: 1   MSMEIEPPLQVRYYALMKEESESKNGRWVGEEEEREVVEECEEEEELPVIDLGLLKMGNS 60

Query: 61  EREKCISEMVEAARNWGFFQVLNHGVPERVLKGMMYEQKKVFNQPFAKKSLSNVLNLAGT 120
           EREKC SEMVEAARNWGFFQVLNHGVPERVLKGMMYEQ+KVFNQPFAKKSLSNVLN+AGT
Sbjct: 61  EREKCKSEMVEAARNWGFFQVLNHGVPERVLKGMMYEQEKVFNQPFAKKSLSNVLNMAGT 120

Query: 121 YRWGNPVAMSPSQISWSEAFHMAVLEVSNLEEHVTLSFKIYGLPTMEAVVKKFGRLAESI 180
           YRWGNPVAMSPSQISWSEAFHMAVLEVSNL EHVTL       PTMEAVVKKFGRLAESI
Sbjct: 121 YRWGNPVAMSPSQISWSEAFHMAVLEVSNLGEHVTLR------PTMEAVVKKFGRLAESI 180

Query: 181 AEILGQSLGIKACYFKERCEKGRSSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQAQV 240
           AEILGQSLGIK+ YFKERCEKGRSSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQAQV
Sbjct: 181 AEILGQSLGIKSSYFKERCEKGRSSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQAQV 240

Query: 241 SGLQLMKDGKWFAVKPNPKALLVNIGDLFQVVSNDAFRSLKHRVVASEAVERFSFAYFYS 300
           SGLQLMKDGKWF+VKPNPKALLVNIGDLFQVVSNDAFRSLKHRVVASEAVERFSFAYFYS
Sbjct: 241 SGLQLMKDGKWFSVKPNPKALLVNIGDLFQVVSNDAFRSLKHRVVASEAVERFSFAYFYS 300

Query: 301 PSDDVMIESCTKPSIYRQFSYREYRQQIEKDVEKTGNKVGLPRFFLHNMLPHLGQ 356
           PSDDVMIES TKPSIYRQFSY+EYRQQIEKDVEKTGNKVGLPRF LHNMLPHLGQ
Sbjct: 301 PSDDVMIESYTKPSIYRQFSYKEYRQQIEKDVEKTGNKVGLPRFLLHNMLPHLGQ 349

[Close]

BLAST of CmoCh16G009200.1 vs. ExPASy TrEMBL
Match: A0A6J1FQ56 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita moschata OX=3662 GN=LOC111447223 PE=3 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 4.0e-148
Identity = 268/350 (76.57%), Postives = 293/350 (83.71%), Query Frame = 0


Query: 1   MSMEIEPPLHVRYYALMKEESESKNGRWVGEEEEREVVEECEEEEELPVIDLGLLKMGNS 60
           +SM+I+PPL V Y ALMK+E E K+GR   E E        E  E+LPVIDLGLLKMGN 
Sbjct: 13  LSMDIDPPLQVCYKALMKDEVEGKSGRVEDEVE--------ETYEDLPVIDLGLLKMGNL 72

Query: 61  EREKCISEMVEAARNWGFFQVLNHGVPERVLKGMMYEQKKVFNQPFAKKSLSNVLNLAGT 120
           EREKC  E+VEAARNWGFFQV+NHGVPER LK ++YEQKKVF  PFA KSL N LNL GT
Sbjct: 73  EREKCKKEIVEAARNWGFFQVMNHGVPERALKALLYEQKKVFYHPFAHKSLGNFLNLGGT 132

Query: 121 YRWGNPVAMSPSQISWSEAFHMAVLEVSNLEEHVTLSFKIYGLPTMEAVVKKFGRLAESI 180
           YRWGNP+A+SPSQISWSEAFH+AVL+VS+LEEH TL        TME V KKFG LAESI
Sbjct: 133 YRWGNPLALSPSQISWSEAFHIAVLDVSSLEEHATLR------STMETVAKKFGSLAESI 192

Query: 181 AEILGQSLGIKACYFKERCEKGRSSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQAQV 240
           AEILGQSLGIK  YFKERCEKG+SS RLNRYPPCPFAS+VYGLIPHTDSDYLTILYQA  
Sbjct: 193 AEILGQSLGIKWSYFKERCEKGKSSLRLNRYPPCPFASKVYGLIPHTDSDYLTILYQAHT 252

Query: 241 SGLQLMKDGKWFAVKPNPKALLVNIGDLFQVVSNDAFRSLKHRVVASEAVERFSFAYFYS 300
           SGLQLM+DG WF VKPNP+ LLVNIGDL QVVSND F+S+KHRVVASE  ERFSFAYFY 
Sbjct: 253 SGLQLMRDGTWFPVKPNPQYLLVNIGDLLQVVSNDVFKSIKHRVVASEEDERFSFAYFYC 312

Query: 301 PSDDVMIESCTKPSIYRQFSYREYRQQIEKDVEKTGNKVGLPRFFLHNML 351
           PS+DVMIESC KPSIY+QFSY+EYRQQIEKDVEKTGNKVGL RFFLH+ML
Sbjct: 313 PSEDVMIESCMKPSIYKQFSYKEYRQQIEKDVEKTGNKVGLSRFFLHHML 348

[Close]

BLAST of CmoCh16G009200.1 vs. ExPASy TrEMBL
Match: A0A6J1JF15 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita maxima OX=3661 GN=LOC111484442 PE=3 SV=1)

HSP 1 Score: 530.4 bits (1365), Expect = 5.7e-147
Identity = 266/348 (76.44%), Postives = 294/348 (84.48%), Query Frame = 0


Query: 3   MEIEPPLHVRYYALMKEESESKNGRWVGEEEEREVVEECEEEEELPVIDLGLLKMGNSER 62
           M+I+PPL V Y ALMK+E E KNGR V +E E       E  E+LPVIDLGLLKMGN ER
Sbjct: 1   MDIDPPLQVCYNALMKDEVEGKNGR-VDDEVE-------ETYEDLPVIDLGLLKMGNLER 60

Query: 63  EKCISEMVEAARNWGFFQVLNHGVPERVLKGMMYEQKKVFNQPFAKKSLSNVLNLAGTYR 122
           EKC  E+VEAARNWGFFQV+NHGVPER LK ++YEQKKVF  PFA+KSL N LNL GTYR
Sbjct: 61  EKCKKEIVEAARNWGFFQVMNHGVPERALKALLYEQKKVFYHPFAQKSLGNFLNLGGTYR 120

Query: 123 WGNPVAMSPSQISWSEAFHMAVLEVSNLEEHVTLSFKIYGLPTMEAVVKKFGRLAESIAE 182
           WGNP+A+SPSQ+SWSEAFH+ VL+VS+LEEHVTL        TME V KKFG +AESIAE
Sbjct: 121 WGNPLALSPSQMSWSEAFHIGVLDVSSLEEHVTLR------STMETVAKKFGSIAESIAE 180

Query: 183 ILGQSLGIKACYFKERCEKGRSSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQAQVSG 242
           ILGQSLGIK  YFKERCEKG+SS RLNRYPPCPFAS+VYGLIPHTDSDYLTILYQA  SG
Sbjct: 181 ILGQSLGIKWSYFKERCEKGKSSLRLNRYPPCPFASKVYGLIPHTDSDYLTILYQAHTSG 240

Query: 243 LQLMKDGKWFAVKPNPKALLVNIGDLFQVVSNDAFRSLKHRVVASEAVERFSFAYFYSPS 302
           LQLM+DG WF VKPNP+ LLVNIGDL QVVSND F+S+KHRVVASE  ERFSFAYFY PS
Sbjct: 241 LQLMRDGTWFPVKPNPQYLLVNIGDLLQVVSNDVFKSIKHRVVASEEDERFSFAYFYCPS 300

Query: 303 DDVMIESCTKPSIYRQFSYREYRQQIEKDVEKTGNKVGLPRFFLHNML 351
           +DVMIESC KPSIY+QFSY+EYRQQIEKDVEKTGNKVGL RFFLH++L
Sbjct: 301 EDVMIESCMKPSIYKQFSYKEYRQQIEKDVEKTGNKVGLSRFFLHHVL 334

[Close]

BLAST of CmoCh16G009200.1 vs. ExPASy TrEMBL
Match: A0A6J1E1P5 (gibberellin 2-beta-dioxygenase 8-like OS=Momordica charantia OX=3673 GN=LOC111025151 PE=3 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 7.8e-144
Identity = 262/350 (74.86%), Postives = 302/350 (86.29%), Query Frame = 0


Query: 3   MEIEPPLHVRYYALMKEE-SESKNGRWVGEEEEREVVEECEEEEELPVIDLGLLKMGNSE 62
           MEIEPPL V Y ALMK++  E +NGR + E+E   VVEEC   +ELP+IDLGLLKMGN E
Sbjct: 1   MEIEPPLQVFYNALMKKDGEEGENGR-ILEDERELVVEEC---DELPLIDLGLLKMGNLE 60

Query: 63  REKCISEMVEAARNWGFFQVLNHGVPERVLKGMMYEQKKVFNQPFAKKSLSNVLNLAGTY 122
           REKC  ++VEAARNWGFFQV+NHGVPER LK +M+EQKKVFNQPF KKSL N +NL+G+Y
Sbjct: 61  REKCKRQIVEAARNWGFFQVVNHGVPERALKAVMFEQKKVFNQPFEKKSLRNFMNLSGSY 120

Query: 123 RWGNPVAMSPSQISWSEAFHMAVLEVSNLEEHVTLSFKIYGLPTMEAVVKKFGRLAESIA 182
           RWGN  A+SP+QISWSEAFH+AVLEV +LE+   LS +     T+E VVKKFG LAESIA
Sbjct: 121 RWGNSQAISPTQISWSEAFHIAVLEVPSLEDQY-LSLR----STVEGVVKKFGGLAESIA 180

Query: 183 EILGQSLGIKACYFKERCEKGRSSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQ-AQV 242
           EILGQSLG+K+ YF+E C++GRSSFRLNRYPPCPFAS+VYGL+PHTD+D+LTILYQ  Q+
Sbjct: 181 EILGQSLGMKSSYFEESCKRGRSSFRLNRYPPCPFASKVYGLVPHTDTDFLTILYQPPQI 240

Query: 243 SGLQLMKDGKWFAVKPNPKALLVNIGDLFQVVSNDAFRSLKHRVVASEAVERFSFAYFYS 302
           + L LMKDG W AVKPNP+ALLVNIGDL QVVSND F+SLKHRV+ASEAVERFSFAYFY 
Sbjct: 241 NALHLMKDGNWLAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVIASEAVERFSFAYFYC 300

Query: 303 PSDDVMIESCTKPSIYRQFSYREYRQQIEKDVEKTGNKVGLPRFFLHNML 351
           PSDDVMIESCTKPSIYRQFSY+EYRQQIEKDVEKTG+KVGLPRF LHN++
Sbjct: 301 PSDDVMIESCTKPSIYRQFSYKEYRQQIEKDVEKTGDKVGLPRFLLHNIM 341

[Close]

BLAST of CmoCh16G009200.1 vs. TAIR 10
Match: AT4G21200.1 (gibberellin 2-oxidase 8 )

HSP 1 Score: 322.0 bits (824), Expect = 6.0e-88
Identity = 170/344 (49.42%), Postives = 224/344 (65.12%), Query Frame = 0


Query: 5   IEPPLHVRYYALMKEESESKNGRWVGEEEEREVVEECEEEEELPVIDLG-LLKMGNSERE 64
           ++PP +  Y  L+  +   K+   V E      V    EE ELPVID+  L+     ERE
Sbjct: 1   MDPPFNEIYNNLLYNQITKKDND-VSEIPFSFSVTAVVEEVELPVIDVSRLIDGAEEERE 60

Query: 65  KCISEMVEAARNWGFFQVLNHGVPERVLKGMMYEQKKVFNQPFAKKSLSNVLNLAGTYRW 124
           KC   +  A+R WGFFQV+NHG+   VL+ M  EQ +VF +PF KKS S   + AG+YRW
Sbjct: 61  KCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFREPFDKKSKSEKFS-AGSYRW 120

Query: 125 GNPVAMSPSQISWSEAFHMAVLEVSNLEEHVTLSFKIYGLPTMEAVVKKFGRLAESIAEI 184
           G P A S  Q+SWSEAFH+ + ++S+ ++  TLS       TME    +   LA  +AE+
Sbjct: 121 GTPSATSIRQLSWSEAFHVPMTDISDNKDFTTLS------STMEKFASESEALAYMLAEV 180

Query: 185 LGQSLGIKACYFKERCEKGRSSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQAQVSGL 244
           L +  G  + +FKE C +     R+NRYPPCP  S+VYGL+PHTDSD+LTILYQ QV GL
Sbjct: 181 LAEKSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVYGLMPHTDSDFLTILYQDQVGGL 240

Query: 245 QLMKDGKWFAVKPNPKALLVNIGDLFQVVSNDAFRSLKHRVVASEAVERFSFAYFYSPSD 304
           QL+KD +W AVKPNPKAL++NIGDLFQ  SN  ++S++HRV+ +  VERFS AYF  PS 
Sbjct: 241 QLIKDNRWIAVKPNPKALIINIGDLFQAWSNGMYKSVEHRVMTNPKVERFSTAYFMCPSY 300

Query: 305 DVMIESCTKPSIYRQFSYREYRQQIEKDVEKTGNKVGLPRFFLH 348
           D +IE  +    YR FS+RE+RQQ+++DV+K G KVGLPRF  H
Sbjct: 301 DAVIECSSDRPAYRNFSFREFRQQVQEDVKKFGFKVGLPRFLNH 336

[Close]

BLAST of CmoCh16G009200.1 vs. TAIR 10
Match: AT1G50960.1 (gibberellin 2-oxidase 7 )

HSP 1 Score: 276.9 bits (707), Expect = 2.2e-74
Identity = 137/303 (45.21%), Postives = 204/303 (67.33%), Query Frame = 0


Query: 46  ELPVIDLGLLKMGNS-EREKCISEMVEAARNWGFFQVLNHGVPERVLKGMMYEQKKVFNQ 105
           +LPVIDL  L  G   +R++C+ +MV AA+ WGFFQ++NHG+P+ V + M+ E+KK+F+Q
Sbjct: 39  KLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQ 98

Query: 106 PFAKKSLSNVLNLA-GTYRWGNPVAMSPSQISWSEAFHMAVLEVSNLEEHVTLSFKIYGL 165
           PF+ K      +L+  +YRWGNP A SP+Q S SEAFH+ + EVS + +       I   
Sbjct: 99  PFSVKVRERFSDLSKNSYRWGNPSATSPAQYSVSEAFHIILSEVSRISDDRNNLRTI--- 158

Query: 166 PTMEAVVKKFGRLAESIAEILGQSLGIKACYFKERCEKGRSSFRLNRYPPCPFASQVYGL 225
             +E  V++  R+A+ I EILG+ + + + YF+   E   S  RLN+Y P  F S+V+GL
Sbjct: 159 --VETYVQEIARVAQMICEILGKQVNVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGL 218

Query: 226 IPHTDSDYLTILYQAQVSGLQLMKDGKWFAVKPNPKALLVNIGDLFQVVSNDAFRSLKHR 285
           +PHTD+ +LTIL Q Q+ GL+L  +G+W +VKP  +AL VNIGD+FQ +SN  ++S++HR
Sbjct: 219 VPHTDTSFLTILSQDQIGGLELENNGQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHR 278

Query: 286 VVASEAVERFSFAYFYSPSDDVMIESCTKPSIYRQFSYREYRQQIEKDVEKTGNKVGLPR 345
           V++   +ER S A+F  P  +  I+    P  YR+FS+REY++Q E DV++TG+KVGL R
Sbjct: 279 VISPANIERMSIAFFVCPYLETEIDCFGYPKKYRRFSFREYKEQSEHDVKETGDKVGLSR 336

Query: 346 FFL 347
           F +
Sbjct: 339 FLI 336

[Close]

BLAST of CmoCh16G009200.1 vs. TAIR 10
Match: AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 157.5 bits (397), Expect = 1.9e-38
Identity = 95/292 (32.53%), Postives = 143/292 (48.97%), Query Frame = 0


Query: 45  EELPVIDLGLLKMGNSEREKCISEMVEAARNWGFFQVLNHGVPERVLKGMMYEQKKVFNQ 104
           + +P+IDL  L   N  R   I++   A  + GFFQ+ NHGVPE  +K MM   ++ F Q
Sbjct: 40  DSIPLIDLHDLHGPN--RADIINQFAHACSSCGFFQIKNHGVPEETIKKMMNAAREFFRQ 99

Query: 105 PFAKKSLSNVLNLAGTYRWGNPVAMSPSQIS-WSEAFHM---AVLEVSNLEEHVTLSFKI 164
             +++      +   T R      +S  ++S W +   +    + +  N      +SF+ 
Sbjct: 100 SESERVKHYSADTKKTTRLSTSFNVSKEKVSNWRDFLRLHCYPIEDFINEWPSTPISFR- 159

Query: 165 YGLPTMEAVVKKFGRLAESIAEILGQSLGIKACYFKERCEKGRSSFRLNRYPPCPFASQV 224
                          L  ++ E + +SLG+          K      +N YP CP     
Sbjct: 160 ---EVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIGKHGQHMAINYYPRCPQPELT 219

Query: 225 YGLIPHTDSDYLTILYQAQVSGLQLMKDGKWFAVKPNPKALLVNIGDLFQVVSNDAFRSL 284
           YGL  H D++ +T+L Q +VSGLQ+ KDGKW AV P P   +VN+GD  QV+SN+ ++S+
Sbjct: 220 YGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNTFIVNLGDQMQVISNEKYKSV 279

Query: 285 KHRVVASEAVERFSFAYFYSPSDDVMI--------ESCTKPSIYRQFSYREY 325
            HR V +  +ER S   FY PS+D +I        E    P+IYR F+Y EY
Sbjct: 280 LHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDSPAIYRNFTYAEY 325

[Close]

BLAST of CmoCh16G009200.1 vs. TAIR 10
Match: AT5G05600.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 156.4 bits (394), Expect = 4.3e-38
Identity = 109/342 (31.87%), Postives = 174/342 (50.88%), Query Frame = 0


Query: 2   SMEIEPPLHVRYYALMKEESESKNGRWVGEEEER-EVVEECEEEEELPVIDL-GLLKMGN 61
           SM+  P   VR  +L +    S   R++     R    E+      +P+IDL GL     
Sbjct: 16  SMDEWPEPIVRVQSLAESNLSSLPDRYIKPASLRPTTTEDAPTATNIPIIDLEGLFSEEG 75

Query: 62  SEREKCISEMVEAARNWGFFQVLNHGVPERVLKGMMYEQKKVFNQPF-AKKSLSNVLNLA 121
              +  ++ + EA R WGFFQV+NHGV   ++       ++ F+ P  AK++ S   N  
Sbjct: 76  LSDDVIMARISEACRGWGFFQVVNHGVKPELMDAARENWREFFHMPVNAKETYS---NSP 135

Query: 122 GTYR-WGNPVAMSP-SQISWSEAFHMAVLEVSNLEEHVTLSFKIYGLPTMEAVVKKFG-- 181
            TY  +G+ + +   + + WS+ + + +L     + +   SF     PT+  V+ ++G  
Sbjct: 136 RTYEGYGSRLGVEKGASLDWSDYYFLHLLPHHLKDFNKWPSFP----PTIREVIDEYGEE 195

Query: 182 --RLAESIAEILGQSLGIKACYFKERC--EKGRSSFRLNRYPPCPFASQVYGLIPHTDSD 241
             +L+  I  +L  +LG+K   F+E    E   +  R+N YP CP      GL PH+D  
Sbjct: 196 LVKLSGRIMRVLSTNLGLKEDKFQEAFGGENIGACLRVNYYPKCPRPELALGLSPHSDPG 255

Query: 242 YLTILY-QAQVSGLQLMKDGKWFAVKPNPKALLVNIGDLFQVVSNDAFRSLKHRVVASEA 301
            +TIL    QV GLQ+ KD  W  VKP+P A +VNIGD  Q++SN  ++S++HRV+ +  
Sbjct: 256 GMTILLPDDQVFGLQVRKDDTWITVKPHPHAFIVNIGDQIQILSNSTYKSVEHRVIVNSD 315

Query: 302 VERFSFAYFYSPSDDVMIE------SCTKPSIYRQFSYREYR 326
            ER S A+FY+P  D+ I+      S   P +Y   ++ +YR
Sbjct: 316 KERVSLAFFYNPKSDIPIQPLQELVSTHNPPLYPPMTFDQYR 350

[Close]

BLAST of CmoCh16G009200.1 vs. TAIR 10
Match: AT3G21420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 151.8 bits (382), Expect = 1.1e-36
Identity = 101/324 (31.17%), Postives = 161/324 (49.69%), Query Frame = 0


Query: 16  LMKEESESKNGRWVGEEEEREVVEECEE----EEELPVIDLG-LLKMGNSEREKCISEMV 75
           L+K +      R++ EE ER VV    +      ++PVIDL  L K  N +    I ++ 
Sbjct: 20  LIKSKPNKVPERFIREEYERGVVVSSLKTHHLHHQIPVIDLSKLSKPDNDDFFFEILKLS 79

Query: 76  EAARNWGFFQVLNHGVPERVLKGMMYEQKKVFNQPFAKKSLSNVLNLAGTYRWGNPVAMS 135
           +A  +WGFFQV+NHG+   V++ +     + F+ P  +K     +       +G     S
Sbjct: 80  QACEDWGFFQVINHGIEVEVVEDIEEVASEFFDMPLEEKK-KYPMEPGTVQGYGQAFIFS 139

Query: 136 PSQ-ISWSEAFHMAVLEVSNLEEHVTLSFKIYGLPTMEAVVKKFGRLAESIAEILGQSLG 195
             Q + W   F + V         +  S       ++E   K+   L + + + +  SLG
Sbjct: 140 EDQKLDWCNMFALGVHPPQIRNPKLWPSKPARFSESLEGYSKEIRELCKRLLKYIAISLG 199

Query: 196 IKACYFKERCEKGRSSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQAQVS--GLQLMK 255
           +K   F+E   +   + R+N YPPC     V GL PH+D   LT+L Q++ S  GLQ++K
Sbjct: 200 LKEERFEEMFGEAVQAVRMNYYPPCSSPDLVLGLSPHSDGSALTVLQQSKNSCVGLQILK 259

Query: 256 DGKWFAVKPNPKALLVNIGDLFQVVSNDAFRSLKHRVVASEAVERFSFAYFYSPSDDVMI 315
           D  W  VKP P AL++NIGD  +V+SN  ++S++HR V +   ER +   FY+P+ +V I
Sbjct: 260 DNTWVPVKPLPNALVINIGDTIEVLSNGKYKSVEHRAVTNREKERLTIVTFYAPNYEVEI 319

Query: 316 ESC-------TKPSIYRQFSYREY 325
           E         T P  YR +++ +Y
Sbjct: 320 EPMSELVDDETNPCKYRSYNHGDY 342

The following BLAST results are available for this feature:

BLAST of CmoCh16G009200.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49561	8.4e-87	49.42	Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 ...	[more]
Q7XP65	9.6e-75	44.41	Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]
Q9C6I4	3.1e-73	45.21	Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 ...	[more]
Q8LGZ9	7.9e-61	43.22	Gibberellin 2-beta-dioxygenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]
Q9ZSA7	2.7e-37	32.53	Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1	[more]

BLAST of CmoCh16G009200.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F0S8	7.1e-198	98.03	gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita moschata OX=3662 GN=LOC111438...	[more]
A0A6J1JAT6	1.9e-190	94.93	gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita maxima OX=3661 GN=LOC11148329...	[more]
A0A6J1FQ56	4.0e-148	76.57	gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita moschata OX=3662 GN=LOC111447...	[more]
A0A6J1JF15	5.7e-147	76.44	gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita maxima OX=3661 GN=LOC11148444...	[more]
A0A6J1E1P5	7.8e-144	74.86	gibberellin 2-beta-dioxygenase 8-like OS=Momordica charantia OX=3673 GN=LOC11102...	[more]

BLAST of CmoCh16G009200.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G21200.1	6.0e-88	49.42	gibberellin 2-oxidase 8	[more]
AT1G50960.1	2.2e-74	45.21	gibberellin 2-oxidase 7	[more]
AT4G10490.1	1.9e-38	32.53	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G05600.1	4.3e-38	31.87	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G21420.1	1.1e-36	31.17	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 47..144 e-value: 1.5E-15 score: 58.0
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 10..326 e-value: 6.9E-77 score: 261.2
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 206..301 e-value: 4.2E-23 score: 81.7
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 36..339
None	No IPR available	PANTHER	PTHR47990:SF19	20-OXIDASE, PUTATIVE-RELATED	coord: 36..339
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 40..328
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 202..301 score: 12.526845

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmoCh16G009200	CmoCh16G009200	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh16G009200.1:exon:10189	CmoCh16G009200.1:exon:10189	exon
CmoCh16G009200.1:exon:10188	CmoCh16G009200.1:exon:10188	exon
CmoCh16G009200.1:exon:10187	CmoCh16G009200.1:exon:10187	exon
CmoCh16G009200.1:exon:10186	CmoCh16G009200.1:exon:10186	exon
CmoCh16G009200.1:exon:10185	CmoCh16G009200.1:exon:10185	exon

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh16G009200.1:three_prime_utr	CmoCh16G009200.1:three_prime_utr	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh16G009200.1:cds	CmoCh16G009200.1:cds_5	CDS
CmoCh16G009200.1:cds	CmoCh16G009200.1:cds_4	CDS
CmoCh16G009200.1:cds	CmoCh16G009200.1:cds_3	CDS
CmoCh16G009200.1:cds	CmoCh16G009200.1:cds_2	CDS
CmoCh16G009200.1:cds	CmoCh16G009200.1:cds	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh16G009200.1:five_prime_utr	CmoCh16G009200.1:five_prime_utr	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmoCh16G009200.1	CmoCh16G009200.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding