CmoCh14G002680.1 (mRNA) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh14G002680.1
Type	mRNA
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Ribosome production factor 2 homolog
Location	Cmo_Chr14: 1210656 .. 1213069 (-)
Sequence length	1395
RNA-Seq Expression	CmoCh14G002680.1
Synteny	CmoCh14G002680.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTGCAGTCGCCGGAAGTGTCGATATTCTTCTGAGCATCAAAACCCTAAATCATCTCCGTCATCTCGTCGTTGTGGATCATGTTGAAGATTAGAACTCCCAAGAATGGAAAAGTTAGACGAGAGCTTGAGAAACGTGCCCCCAAAATTGTAAGTTCTTCCAGTCCTGTCTGTATATCTTCATGCTTCGGGAGATAGTTTGTACTGGACGATTAACAGCATGTCAAAGAAGTTTGTATAGGCTGTGAATTGTTATTTACTTCCCGTATTGATGGATTTGGTTATCGATTTGAATCGCAGGTTGAGACGGGGAAGAAGACGCTGATACTTCATGGCACAAAAACAAGCGGCGTGTTGAATGCAGTACTGACGGAACTCTATCACCTGAAGAAAGAAAGTTCCGTGAAGTATTCTCGTAAGAATGATGGAATTAAACCATTTGAAAGTGGCGGCGATTCGTCTTTGGAATTTTTCTCTCTCAAAACTGATTGCAGTATTATCGTGGTGCGTACACTTGTGAATGCTTAATATTCCATGCGTCGGTTATTTACTTGGCTTAATTTCTTGGAGCTCAGCAGCAAGTAACGTACTTTTTTGAGTTTTGTATGTGCTTTTACAATTCAGTTTTCCCATCCGGAGGCAATTTCTGTACTCTTCATCACAGAGACTTTGATATAAAATTAATTCTAGCCGTTTAGTGATTCGTTTAAGGTTTGATTCTTTTGGATGAGTACTTCTCGTTTTCCCTAAATGGTTTTTGATCGAAGAACTTAGCAGTCCTCAAACAGATGAACCTATGTAGAAAAATATAGTCACGATGTGCATTTTTGTTGGGGTAAGGTGAGCCGCCAAGAATTTAGAGAACTTCAATTTAGGAGGCACCCTATTTTGAGGGAAACCTCCGTCTTATTGTGTTCAAGTTTTGTATCTCTTTTCCTCTGTTATTATAAAACAGTAAACAGCTTCGTGCTTTTATGTCTCCAGTTATTTATTGTTGTATTTGTTGCACCTCTTTTGCAGTATGGTTCTCACTCAAAGAAACGACCTGATAACCTTGTCATAGGGAGAACCTATGATCACAAAATCTATGACCTAGTTGAGGTTGGGATTGAAAATTTCAAGTCAATGCATTCTTTCACATATGATAAGAAACTCTCGCCACGGGTGGGGTCAAAGCCTTTCATTACTTTCATTGGGGAAGGATTTGAGAATGTGGAAGAGCTAAAGCATTTAAAGGAAGTTTTGCTTGATTTATTCCGGGGGGAGGTAAAAACCCTGCAGCTTTAGCTGACTTGAGTTTGAATGTTTAGATATAAAAGATATCTATTGTCTTTGCAGGTTGTAGAGAATTTAAATCTTGCAGGGTTGGACCGTGTGTATGTATGTACAGCCATCTCACCGAATAAAGTATTTCTCACTCATTGTGCATTGCGATTGAAGAAGTCAGGTTCAGTAGTTCCAAGAATGGAACTAGTTGAAGTTGGTCCTTCCATGGATATGGTGGTTCGCCGCCACCGACTTCCTAGTGATGGTCTAAGGAAGGAAGCCATGAAAACAGCCAAGGATCAGGCTAAGAAGAAGGTTTAATGGCTATCTTTTAAATGCATGCATTTCATTTTTTCTATTTAGCCGTTTGTCTAATTTTGATGTTTACTTCTTCCAGGAGAAGAATGTTAGCCAGGATGCAATTCAGGGCAAGATCGGAAGGATCTATATTCCTGATCAAAAGGTGTGCACATCTCTGTCTGTATATGTCTTTTCTTGTTAGAGGGCTTGCTACTGTCACAATGCATGCATATCCTCTAGATTGGAAGTGGTTCATGTAATTGAACTCTTCTCTTGTCATGTTAGGTTCATGATATGAATTCTCATATCATGTTTATCACCAAAGTAGGTACTTCATTATGTCATCCGGTACAAATTTGCAAGTCATTTGAATAAACTTGATTTTCAACATTGACTCATAGGTTGGAGATATGGCTATACCCGACAAATCAAAGGGAGTGAAGAGAGAACGACGTGAAGCGAAAATGAAAAATAAAGATGACGGACCAGCCTCTAAAAAACCAAAGGAAGATTCAGAAAAGCCGAAGGAAAATTCGGAATAGTTCAATATTAGTGGAATAGTTTTGATCCGATTCTCCTCTTGCTAACTTGAGTCAGCTGTTGTGAGTTGCAGGGTAGCACGCCAAATATATGGAGAGAGCAGACAAATTATTTATTGCTTATATACAATTTTGTTTTTGTTCTTCAATCTGATTCTTACATGCTGAGCCTATCTTTTATCATCTTAATCATTCAAGCTCTTTCAAAGTTTGATCGTGAACCTCATTGATTCTGTTGTTGTGTAGCAAACAGAAGTTCTTTCAAAGTATGTATATGTTAGCAAGATGAGCTTACGATAGCATTGCAGTGCA

mRNA sequence

TTGCAGTCGCCGGAAGTGTCGATATTCTTCTGAGCATCAAAACCCTAAATCATCTCCGTCATCTCGTCGTTGTGGATCATGTTGAAGATTAGAACTCCCAAGAATGGAAAAGTTAGACGAGAGCTTGAGAAACGTGCCCCCAAAATTGTTGAGACGGGGAAGAAGACGCTGATACTTCATGGCACAAAAACAAGCGGCGTGTTGAATGCAGTACTGACGGAACTCTATCACCTGAAGAAAGAAAGTTCCGTGAAGTATTCTCGTAAGAATGATGGAATTAAACCATTTGAAAGTGGCGGCGATTCGTCTTTGGAATTTTTCTCTCTCAAAACTGATTGCAGTATTATCGTGTATGGTTCTCACTCAAAGAAACGACCTGATAACCTTGTCATAGGGAGAACCTATGATCACAAAATCTATGACCTAGTTGAGGTTGGGATTGAAAATTTCAAGTCAATGCATTCTTTCACATATGATAAGAAACTCTCGCCACGGGTGGGGTCAAAGCCTTTCATTACTTTCATTGGGGAAGGATTTGAGAATGTGGAAGAGCTAAAGCATTTAAAGGAAGTTTTGCTTGATTTATTCCGGGGGGAGGTTGTAGAGAATTTAAATCTTGCAGGGTTGGACCGTGTGTATGTATGTACAGCCATCTCACCGAATAAAGTATTTCTCACTCATTGTGCATTGCGATTGAAGAAGTCAGGTTCAGTAGTTCCAAGAATGGAACTAGTTGAAGTTGGTCCTTCCATGGATATGGTGGTTCGCCGCCACCGACTTCCTAGTGATGGTCTAAGGAAGGAAGCCATGAAAACAGCCAAGGATCAGGCTAAGAAGAAGGAGAAGAATGTTAGCCAGGATGCAATTCAGGGCAAGATCGGAAGGATCTATATTCCTGATCAAAAGGTTCATGATATGAATTCTCATATCATGTTTATCACCAAAGTTGGAGATATGGCTATACCCGACAAATCAAAGGGAGTGAAGAGAGAACGACGTGAAGCGAAAATGAAAAATAAAGATGACGGACCAGCCTCTAAAAAACCAAAGGAAGATTCAGAAAAGCCGAAGGAAAATTCGGAATAGTTCAATATTAGTGGAATAGTTTTGATCCGATTCTCCTCTTGCTAACTTGAGTCAGCTGTTGTGAGTTGCAGGGTAGCACGCCAAATATATGGAGAGAGCAGACAAATTATTTATTGCTTATATACAATTTTGTTTTTGTTCTTCAATCTGATTCTTACATGCTGAGCCTATCTTTTATCATCTTAATCATTCAAGCTCTTTCAAAGTTTGATCGTGAACCTCATTGATTCTGTTGTTGTGTAGCAAACAGAAGTTCTTTCAAAGTATGTATATGTTAGCAAGATGAGCTTACGATAGCATTGCAGTGCA

Coding sequence (CDS)

ATGTTGAAGATTAGAACTCCCAAGAATGGAAAAGTTAGACGAGAGCTTGAGAAACGTGCCCCCAAAATTGTTGAGACGGGGAAGAAGACGCTGATACTTCATGGCACAAAAACAAGCGGCGTGTTGAATGCAGTACTGACGGAACTCTATCACCTGAAGAAAGAAAGTTCCGTGAAGTATTCTCGTAAGAATGATGGAATTAAACCATTTGAAAGTGGCGGCGATTCGTCTTTGGAATTTTTCTCTCTCAAAACTGATTGCAGTATTATCGTGTATGGTTCTCACTCAAAGAAACGACCTGATAACCTTGTCATAGGGAGAACCTATGATCACAAAATCTATGACCTAGTTGAGGTTGGGATTGAAAATTTCAAGTCAATGCATTCTTTCACATATGATAAGAAACTCTCGCCACGGGTGGGGTCAAAGCCTTTCATTACTTTCATTGGGGAAGGATTTGAGAATGTGGAAGAGCTAAAGCATTTAAAGGAAGTTTTGCTTGATTTATTCCGGGGGGAGGTTGTAGAGAATTTAAATCTTGCAGGGTTGGACCGTGTGTATGTATGTACAGCCATCTCACCGAATAAAGTATTTCTCACTCATTGTGCATTGCGATTGAAGAAGTCAGGTTCAGTAGTTCCAAGAATGGAACTAGTTGAAGTTGGTCCTTCCATGGATATGGTGGTTCGCCGCCACCGACTTCCTAGTGATGGTCTAAGGAAGGAAGCCATGAAAACAGCCAAGGATCAGGCTAAGAAGAAGGAGAAGAATGTTAGCCAGGATGCAATTCAGGGCAAGATCGGAAGGATCTATATTCCTGATCAAAAGGTTCATGATATGAATTCTCATATCATGTTTATCACCAAAGTTGGAGATATGGCTATACCCGACAAATCAAAGGGAGTGAAGAGAGAACGACGTGAAGCGAAAATGAAAAATAAAGATGACGGACCAGCCTCTAAAAAACCAAAGGAAGATTCAGAAAAGCCGAAGGAAAATTCGGAATAG

Protein sequence

MLKIRTPKNGKVRRELEKRAPKIVETGKKTLILHGTKTSGVLNAVLTELYHLKKESSVKYSRKNDGIKPFESGGDSSLEFFSLKTDCSIIVYGSHSKKRPDNLVIGRTYDHKIYDLVEVGIENFKSMHSFTYDKKLSPRVGSKPFITFIGEGFENVEELKHLKEVLLDLFRGEVVENLNLAGLDRVYVCTAISPNKVFLTHCALRLKKSGSVVPRMELVEVGPSMDMVVRRHRLPSDGLRKEAMKTAKDQAKKKEKNVSQDAIQGKIGRIYIPDQKVHDMNSHIMFITKVGDMAIPDKSKGVKRERREAKMKNKDDGPASKKPKEDSEKPKENSE

Homology

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BLAST of CmoCh14G002680.1 vs. ExPASy Swiss-Prot
Match: Q9LUG5 (Ribosome production factor 2 homolog OS=Arabidopsis thaliana OX=3702 GN=At3g23620 PE=2 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 1.0e-126
Identity = 227/328 (69.21%), Postives = 276/328 (84.15%), Query Frame = 0


Query: 1   MLKIRTPKNGKVRRELEKRAPKIVETGKKTLILHGTKTSGVLNAVLTELYHLKKESSVKY 60
           M++IRTPK GK +R LE RAPK+VETGKKTLILHGTKTS  L++V+TELY LKK  +++Y
Sbjct: 1   MMEIRTPKTGKAKRVLESRAPKLVETGKKTLILHGTKTSATLSSVMTELYRLKKGGAIRY 60

Query: 61  SRKNDGIKPFESGGDSSLEFFSLKTDCSIIVYGSHSKKRPDNLVIGRTYDHKIYDLVEVG 120
           SR+N+ I+PFESGG++SLEFFS KTDCSI VYGSH+KKRPDNLV+GR YDH++YDL+EVG
Sbjct: 61  SRRNENIRPFESGGETSLEFFSQKTDCSIFVYGSHTKKRPDNLVLGRMYDHQVYDLIEVG 120

Query: 121 IENFKSMHSFTYDKKLSPRVGSKPFITFIGEGFENVEELKHLKEVLLDLFRGEVVENLNL 180
           IENFKS+ +F+YDKK +P  G+KPFI FIGEGFENV ELKHLKEVL DLFRGEVV+NLNL
Sbjct: 121 IENFKSLRAFSYDKKFAPHEGTKPFICFIGEGFENVSELKHLKEVLTDLFRGEVVDNLNL 180

Query: 181 AGLDRVYVCTAISPNKVFLTHCALRLKKSGSVVPRMELVEVGPSMDMVVRRHRLPSDGLR 240
            GLDR YVC+AISP KVFLTHCAL+LKKSGS+VPRMELVEVGPSMD+V+RR+RLP+D L 
Sbjct: 181 TGLDRAYVCSAISPTKVFLTHCALKLKKSGSIVPRMELVEVGPSMDLVIRRNRLPNDSLM 240

Query: 241 KEAMKTAKDQAKKKEKNVSQDAIQGKIGRIYIPDQKVHDMNSHIMFITKVGDMAIPDKSK 300
           KEAM+T+KD+ KKKEKNV QDA+ GK G+IY+PDQK+ +M              + DKSK
Sbjct: 241 KEAMRTSKDKPKKKEKNVDQDAVLGKTGKIYMPDQKLKEMK-------------LFDKSK 300

Query: 301 GVKRERREAKMKNKDDGPASKKPKEDSE 329
           G KRER++AK+K+K++   +KK K  SE
Sbjct: 301 GSKRERKDAKLKHKEE-TVAKKMKVSSE 314

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BLAST of CmoCh14G002680.1 vs. ExPASy Swiss-Prot
Match: Q9AWM9 (Ribosome production factor 2 homolog OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0513800 PE=2 SV=1)

HSP 1 Score: 427.6 bits (1098), Expect = 1.3e-118
Identity = 220/325 (67.69%), Postives = 272/325 (83.69%), Query Frame = 0


Query: 4   IRTPKNGKVRRELEKRAPKIVETGKKTLILHGTKTSGVLNAVLTELYHLKKESSVKYSRK 63
           IR P++ + +REL K APK+VETGKKTLILHGTKTS VLN+VL +L+HLK++++VKYS+K
Sbjct: 5   IRVPRSQRAKRELLKHAPKLVETGKKTLILHGTKTSAVLNSVLADLFHLKRDNAVKYSKK 64

Query: 64  NDGIKPFESGGDSSLEFFSLKTDCSIIVYGSHSKKRPDNLVIGRTYDHKIYDLVEVGIEN 123
           ND I+PFESGG++SLEFFSLKTDCS+IVYGSHSKKRP+NLV+GRTYDH IYDLVEVG+EN
Sbjct: 65  NDNIRPFESGGETSLEFFSLKTDCSLIVYGSHSKKRPNNLVLGRTYDHHIYDLVEVGVEN 124

Query: 124 FKSMHSFTYDKKLSPRVGSKPFITFIGEGFENVEELKHLKEVLLDLFRGEVVENLNLAGL 183
           +KS+ S+ YDKKL+P++GSKPF  FIGE FE+VEELKHLKEVLLDLF+GEVVENLNLAG+
Sbjct: 125 YKSIESYVYDKKLAPKLGSKPFFAFIGEHFESVEELKHLKEVLLDLFKGEVVENLNLAGV 184

Query: 184 DRVYVCTAISPNKVFLTHCALRLKKSGSVVPRMELVEVGPSMDMVVRRHRLPSDGLRKEA 243
           DRV+VCTAISP  V++ HCALRLK+SG+ +PR+ELVEVGPSMD+VVRRHR P + L+KEA
Sbjct: 185 DRVFVCTAISPTTVYMMHCALRLKRSGTSIPRIELVEVGPSMDLVVRRHRYPVESLKKEA 244

Query: 244 MKTAKDQAKKKEKNVSQDAIQGKIGRIYIPDQKVHDMNSHIMFITKVGDMAIPDKSKGVK 303
           MKTA D AKK  KNV++D + GK+G++YIPDQ             ++  M++ +  KG+K
Sbjct: 245 MKTA-DHAKKM-KNVTKDPVHGKLGKVYIPDQ-------------QIAKMSLSNDVKGLK 304

Query: 304 RERREAKMKNKDDGPASKKPKEDSE 329
           RERREAK KNKD    SKK K + E
Sbjct: 305 RERREAK-KNKDH---SKKQKINPE 310

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BLAST of CmoCh14G002680.1 vs. ExPASy Swiss-Prot
Match: Q9JJ80 (Ribosome production factor 2 homolog OS=Mus musculus OX=10090 GN=Rpf2 PE=2 SV=2)

HSP 1 Score: 214.5 bits (545), Expect = 1.8e-54
Identity = 133/324 (41.05%), Postives = 190/324 (58.64%), Query Frame = 0


Query: 3   KIRTPKNGKVRRELEKRAPKIVETGKKTLILHGTKTSGVLNAVLTELYHLKKESSVKYSR 62
           K+  PK  + +R LEKR PK+ E  K  +++ G   +  +  VL ++Y LKK   V Y +
Sbjct: 6   KVLKPKTKRAKRFLEKREPKLTENIKNAMLIKGGNANATVTQVLRDMYALKKPYGVLYKK 65

Query: 63  KNDGIKPFESGGDSSLEFFSLKTDCSIIVYGSHSKKRPDNLVIGRTYDHKIYDLVEVGIE 122
           KN   +PFE    +SLEFFS K+DCS+ ++GSH+KKRP+NLVIGR YD+ + D++E+GIE
Sbjct: 66  KNI-TRPFED--QTSLEFFSKKSDCSLFMFGSHNKKRPNNLVIGRMYDYHVLDMIELGIE 125

Query: 123 NFKSMHSFTYDKKLSPRVGSKPFITFIGEGFENVEELKHLKEVLLDLFRGEVVENLNLAG 182
            F S+      K   P  G+KP + F G+ F+  E+ + LK +L+D FRG  V N+ LAG
Sbjct: 126 KFVSLKDIKTSK--CPE-GTKPMLIFAGDDFDVTEDFRRLKNLLIDFFRGPTVSNVRLAG 185

Query: 183 LDRVYVCTAISPNKVFLTHCALRLKKSGSVVPRMELVEVGPSMDMVVRRHRLPSDGLRKE 242
           L+ V   TA++  KV+     L LKKSG   PR+EL E+GPS+D+V+RR  L SD L K 
Sbjct: 186 LEYVLHFTALN-GKVYFRSYKLLLKKSGCRTPRIELEEMGPSLDLVMRRTHLASDDLYKL 245

Query: 243 AMKTAKDQAKKKEKNVSQDAIQGKIGRIYIPDQKVHDMNSHIMFITKVGDMAIPDKSKGV 302
           +MK  K    KK KN+SQD      GRI++  Q +  + +               K KG+
Sbjct: 246 SMKVPKALKPKKRKNISQDTFGTTFGRIHMQKQDLSKLQTR--------------KMKGL 305

Query: 303 KRERREAKMKNKDDGPASKKPKED 327
           K  +R A+    D G  SK+ K++
Sbjct: 306 K--KRPAENGVDDQGKKSKRIKKN 306

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BLAST of CmoCh14G002680.1 vs. ExPASy Swiss-Prot
Match: Q2YDN6 (Ribosome production factor 2 homolog OS=Bos taurus OX=9913 GN=RPF2 PE=2 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 3.4e-53
Identity = 129/324 (39.81%), Postives = 192/324 (59.26%), Query Frame = 0


Query: 3   KIRTPKNGKVRRELEKRAPKIVETGKKTLILHGTKTSGVLNAVLTELYHLKKESSVKYSR 62
           ++  PK  + +R LEKR PK+ E  K  +++ G   +  +  VL ++Y LKK   + Y +
Sbjct: 6   RVVKPKTKRAKRFLEKREPKLSENIKNAMLIKGGNANSTVTQVLRDVYALKKPYGILYKK 65

Query: 63  KNDGIKPFESGGDSSLEFFSLKTDCSIIVYGSHSKKRPDNLVIGRTYDHKIYDLVEVGIE 122
           KN   +PFE    +SLEFFS K+DCS+ ++GSH+KKRP+NLVIGR YD+ + D++E+GIE
Sbjct: 66  KNI-TRPFED--QTSLEFFSKKSDCSLFMFGSHNKKRPNNLVIGRMYDYHVLDIIELGIE 125

Query: 123 NFKSMHSFTYDKKLSPRVGSKPFITFIGEGFENVEELKHLKEVLLDLFRGEVVENLNLAG 182
            F S+      K   P  G+KP + F G+ F+  E+ + LK +L+D FRG  V N+ LAG
Sbjct: 126 KFVSLKDIKNSK--CPE-GTKPMLIFAGDDFDVTEDYRRLKSLLIDFFRGPTVSNIRLAG 185

Query: 183 LDRVYVCTAISPNKVFLTHCALRLKKSGSVVPRMELVEVGPSMDMVVRRHRLPSDGLRKE 242
           L+ V   TA++  K++     L LKKSG   PR+EL E+GPS+D+V+RR  L SD L K 
Sbjct: 186 LEYVLHFTALN-GKIYFRSYKLLLKKSGCRTPRIELEEMGPSLDLVLRRTHLASDDLYKL 245

Query: 243 AMKTAKDQAKKKEKNVSQDAIQGKIGRIYIPDQKVHDMNSHIMFITKVGDMAIPDKSKGV 302
           +MK  K    KK+KN+S D      GRI++  Q +  + +               K KG+
Sbjct: 246 SMKMPKALKPKKKKNISHDTFGTTYGRIHMQKQDLSKLQTR--------------KMKGL 305

Query: 303 KRERREAKMKNKDDGPASKKPKED 327
           K  +R A+ K +D+   SK+ K++
Sbjct: 306 K--KRPAERKAEDEENKSKRIKKN 306

[Close]

BLAST of CmoCh14G002680.1 vs. ExPASy Swiss-Prot
Match: Q9H7B2 (Ribosome production factor 2 homolog OS=Homo sapiens OX=9606 GN=RPF2 PE=1 SV=2)

HSP 1 Score: 209.5 bits (532), Expect = 5.7e-53
Identity = 130/324 (40.12%), Postives = 191/324 (58.95%), Query Frame = 0


Query: 3   KIRTPKNGKVRRELEKRAPKIVETGKKTLILHGTKTSGVLNAVLTELYHLKKESSVKYSR 62
           ++  PK  + +R LEKR PK+ E  K  +++ G   +  +  VL ++Y LKK   V Y +
Sbjct: 6   RVVKPKTKRAKRFLEKREPKLNENIKNAMLIKGGNANATVTKVLKDVYALKKPYGVLYKK 65

Query: 63  KNDGIKPFESGGDSSLEFFSLKTDCSIIVYGSHSKKRPDNLVIGRTYDHKIYDLVEVGIE 122
           KN   +PFE    +SLEFFS K+DCS+ ++GSH+KKRP+NLVIGR YD+ + D++E+GIE
Sbjct: 66  KNI-TRPFED--QTSLEFFSKKSDCSLFMFGSHNKKRPNNLVIGRMYDYHVLDMIELGIE 125

Query: 123 NFKSMHSFTYDKKLSPRVGSKPFITFIGEGFENVEELKHLKEVLLDLFRGEVVENLNLAG 182
           NF S+      K   P  G+KP + F G+ F+  E+ + LK +L+D FRG  V N+ LAG
Sbjct: 126 NFVSLKDIKNSK--CPE-GTKPMLIFAGDDFDVTEDYRRLKSLLIDFFRGPTVSNIRLAG 185

Query: 183 LDRVYVCTAISPNKVFLTHCALRLKKSGSVVPRMELVEVGPSMDMVVRRHRLPSDGLRKE 242
           L+ V   TA++  K++     L LKKSG   PR+EL E+GPS+D+V+RR  L SD L K 
Sbjct: 186 LEYVLHFTALN-GKIYFRSYKLLLKKSGCRTPRIELEEMGPSLDLVLRRTHLASDDLYKL 245

Query: 243 AMKTAKDQAKKKEKNVSQDAIQGKIGRIYIPDQKVHDMNSHIMFITKVGDMAIPDKSKGV 302
           +MK  K    KK+KN+S D      GRI++  Q +  + +               K KG+
Sbjct: 246 SMKMPKALKPKKKKNISHDTFGTTYGRIHMQKQDLSKLQTR--------------KMKGL 305

Query: 303 KRERREAKMKNKDDGPASKKPKED 327
           K  +R A+   +D    SK+ K++
Sbjct: 306 K--KRPAERITEDHEKKSKRIKKN 306

[Close]

BLAST of CmoCh14G002680.1 vs. ExPASy TrEMBL
Match: A0A6J1F357 (Ribosome production factor 2 homolog OS=Cucurbita moschata OX=3662 GN=LOC111441948 PE=3 SV=1)

HSP 1 Score: 614.4 bits (1583), Expect = 2.9e-172
Identity = 322/335 (96.12%), Postives = 322/335 (96.12%), Query Frame = 0


Query: 1   MLKIRTPKNGKVRRELEKRAPKIVETGKKTLILHGTKTSGVLNAVLTELYHLKKESSVKY 60
           MLKIRTPKNGKVRRELEKRAPKIVETGKKTLILHGTKTSGVLNAVLTELYHLKKESSVKY
Sbjct: 1   MLKIRTPKNGKVRRELEKRAPKIVETGKKTLILHGTKTSGVLNAVLTELYHLKKESSVKY 60

Query: 61  SRKNDGIKPFESGGDSSLEFFSLKTDCSIIVYGSHSKKRPDNLVIGRTYDHKIYDLVEVG 120
           SRKNDGIKPFESGGDSSLEFFSLKTDCSIIVYGSHSKKRPDNLVIGRTYDHKIYDLVEVG
Sbjct: 61  SRKNDGIKPFESGGDSSLEFFSLKTDCSIIVYGSHSKKRPDNLVIGRTYDHKIYDLVEVG 120

Query: 121 IENFKSMHSFTYDKKLSPRVGSKPFITFIGEGFENVEELKHLKEVLLDLFRGEVVENLNL 180
           IENFKSMHSFTYDKKLSPRVGSKPFITFIGEGFENVEELKHLKEVLLDLFRGEVVENLNL
Sbjct: 121 IENFKSMHSFTYDKKLSPRVGSKPFITFIGEGFENVEELKHLKEVLLDLFRGEVVENLNL 180

Query: 181 AGLDRVYVCTAISPNKVFLTHCALRLKKSGSVVPRMELVEVGPSMDMVVRRHRLPSDGLR 240
           AGLDRVYVCTAISPNKVFLTHCALRLKKSGSVVPRMELVEVGPSMDMVVRRHRLPSDGLR
Sbjct: 181 AGLDRVYVCTAISPNKVFLTHCALRLKKSGSVVPRMELVEVGPSMDMVVRRHRLPSDGLR 240

Query: 241 KEAMKTAKDQAKKKEKNVSQDAIQGKIGRIYIPDQKVHDMNSHIMFITKVGDMAIPDKSK 300
           KEAMKTAKDQAKKKEKNVSQDAIQGKIGRIYIPDQ             KVGDMAIPDKSK
Sbjct: 241 KEAMKTAKDQAKKKEKNVSQDAIQGKIGRIYIPDQ-------------KVGDMAIPDKSK 300

Query: 301 GVKRERREAKMKNKDDGPASKKPKEDSEKPKENSE 336
           GVKRERREAKMKNKDDGPASKKPKEDSEKPKENSE
Sbjct: 301 GVKRERREAKMKNKDDGPASKKPKEDSEKPKENSE 322

[Close]

BLAST of CmoCh14G002680.1 vs. ExPASy TrEMBL
Match: A0A6J1J655 (Ribosome production factor 2 homolog OS=Cucurbita maxima OX=3661 GN=LOC111481697 PE=3 SV=1)

HSP 1 Score: 611.7 bits (1576), Expect = 1.8e-171
Identity = 319/335 (95.22%), Postives = 322/335 (96.12%), Query Frame = 0


Query: 1   MLKIRTPKNGKVRRELEKRAPKIVETGKKTLILHGTKTSGVLNAVLTELYHLKKESSVKY 60
           MLK+RTPKNGKVRRELEKRAPKIVETGKKTLILHGTKTSGVLNAVLTELYHLKKESSVKY
Sbjct: 1   MLKVRTPKNGKVRRELEKRAPKIVETGKKTLILHGTKTSGVLNAVLTELYHLKKESSVKY 60

Query: 61  SRKNDGIKPFESGGDSSLEFFSLKTDCSIIVYGSHSKKRPDNLVIGRTYDHKIYDLVEVG 120
           SRKNDGIKPFESGGDSSLEFFSLKTDCSIIVYGSHSKKRPDNLVIGRTYDHKIYDL+EVG
Sbjct: 61  SRKNDGIKPFESGGDSSLEFFSLKTDCSIIVYGSHSKKRPDNLVIGRTYDHKIYDLIEVG 120

Query: 121 IENFKSMHSFTYDKKLSPRVGSKPFITFIGEGFENVEELKHLKEVLLDLFRGEVVENLNL 180
           IENFKSMHSFTYDKKLSPRVGSKPFITFIGEGFENVEELKHLKEVLLDLFRGEVVENLNL
Sbjct: 121 IENFKSMHSFTYDKKLSPRVGSKPFITFIGEGFENVEELKHLKEVLLDLFRGEVVENLNL 180

Query: 181 AGLDRVYVCTAISPNKVFLTHCALRLKKSGSVVPRMELVEVGPSMDMVVRRHRLPSDGLR 240
           AGLDRVYVCTAISPNKVFLTHCALRLKKSGSVVPRMELVEVGPSMDMVVRRHRLPSDGLR
Sbjct: 181 AGLDRVYVCTAISPNKVFLTHCALRLKKSGSVVPRMELVEVGPSMDMVVRRHRLPSDGLR 240

Query: 241 KEAMKTAKDQAKKKEKNVSQDAIQGKIGRIYIPDQKVHDMNSHIMFITKVGDMAIPDKSK 300
           KEAMKTAKDQAKKKEKNVSQDAIQGKIGRIYIPDQ             KVGDMAIPDKSK
Sbjct: 241 KEAMKTAKDQAKKKEKNVSQDAIQGKIGRIYIPDQ-------------KVGDMAIPDKSK 300

Query: 301 GVKRERREAKMKNKDDGPASKKPKEDSEKPKENSE 336
           GVKRERREAKMK+KDDGPASKKPKEDSEKPKENSE
Sbjct: 301 GVKRERREAKMKSKDDGPASKKPKEDSEKPKENSE 322

[Close]

BLAST of CmoCh14G002680.1 vs. ExPASy TrEMBL
Match: A0A6J1KZU6 (Ribosome production factor 2 homolog OS=Cucurbita maxima OX=3661 GN=LOC111498582 PE=3 SV=1)

HSP 1 Score: 583.9 bits (1504), Expect = 4.1e-163
Identity = 299/334 (89.52%), Postives = 316/334 (94.61%), Query Frame = 0


Query: 1   MLKIRTPKNGKVRRELEKRAPKIVETGKKTLILHGTKTSGVLNAVLTELYHLKKESSVKY 60
           MLK+RTPKNGKV+RELEKRAPK+VETGKKTLILHGTKTSGVLN VLTE+YHLKKES+VKY
Sbjct: 1   MLKVRTPKNGKVKRELEKRAPKLVETGKKTLILHGTKTSGVLNEVLTEIYHLKKESAVKY 60

Query: 61  SRKNDGIKPFESGGDSSLEFFSLKTDCSIIVYGSHSKKRPDNLVIGRTYDHKIYDLVEVG 120
           SR+N+GIKPFE+GGDSSLEFFSLKTDCSIIVYGSHSKKRPDNLVI RTYDH IYDL+EVG
Sbjct: 61  SRRNEGIKPFENGGDSSLEFFSLKTDCSIIVYGSHSKKRPDNLVIARTYDHHIYDLIEVG 120

Query: 121 IENFKSMHSFTYDKKLSPRVGSKPFITFIGEGFENVEELKHLKEVLLDLFRGEVVENLNL 180
           IENFKSMHSFTYDKKLSPRVGSKPFITFIGEGFENVEELKHLKEVLLD+FRGEVVEN+NL
Sbjct: 121 IENFKSMHSFTYDKKLSPRVGSKPFITFIGEGFENVEELKHLKEVLLDMFRGEVVENINL 180

Query: 181 AGLDRVYVCTAISPNKVFLTHCALRLKKSGSVVPRMELVEVGPSMDMVVRRHRLPSDGLR 240
           AGLDRVYVCTAISPNKVFLTHCALR+KKSGSVVPRMELVEVGPSMD+VVRRHRLPSDGLR
Sbjct: 181 AGLDRVYVCTAISPNKVFLTHCALRMKKSGSVVPRMELVEVGPSMDLVVRRHRLPSDGLR 240

Query: 241 KEAMKTAKDQAKKKEKNVSQDAIQGKIGRIYIPDQKVHDMNSHIMFITKVGDMAIPDKSK 300
           KEAMKTAKDQAKKKEKNVSQDAI GKIGRIYIPDQ             KVGD+A+PDKSK
Sbjct: 241 KEAMKTAKDQAKKKEKNVSQDAIHGKIGRIYIPDQ-------------KVGDVALPDKSK 300

Query: 301 GVKRERREAKMKNKDDGPASKKPKEDSEKPKENS 335
           GVKRERREAKMKN+DD PASKKPKEDSEKPKE+S
Sbjct: 301 GVKRERREAKMKNRDDMPASKKPKEDSEKPKEDS 321

[Close]

BLAST of CmoCh14G002680.1 vs. ExPASy TrEMBL
Match: A0A6J1FZZ6 (Ribosome production factor 2 homolog OS=Cucurbita moschata OX=3662 GN=LOC111449459 PE=3 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 3.5e-162
Identity = 299/335 (89.25%), Postives = 316/335 (94.33%), Query Frame = 0


Query: 1   MLKIRTPKNGKVRRELEKRAPKIVETGKKTLILHGTKTSGVLNAVLTELYHLKKESSVKY 60
           MLK+RTPKNGKV+RELEKRAPK+VETGKKTLILHGTKTSGVLN VLTE+YHLKKES+VKY
Sbjct: 1   MLKVRTPKNGKVKRELEKRAPKLVETGKKTLILHGTKTSGVLNEVLTEIYHLKKESAVKY 60

Query: 61  SRKNDGIKPFESGGDSSLEFFSLKTDCSIIVYGSHSKKRPDNLVIGRTYDHKIYDLVEVG 120
           SR+N+GIKPFE+GGDSSLEFFSLKTDCSIIVYGSHSKKRPDNLVI RTYDH IYDLVEVG
Sbjct: 61  SRRNEGIKPFENGGDSSLEFFSLKTDCSIIVYGSHSKKRPDNLVIARTYDHHIYDLVEVG 120

Query: 121 IENFKSMHSFTYDKKLSPRVGSKPFITFIGEGFENVEELKHLKEVLLDLFRGEVVENLNL 180
           IENFKSMHSFTYDKKLSPRVGSKPFITFIGEGFENVEELKHLKEVLLD+FRGEVVEN+NL
Sbjct: 121 IENFKSMHSFTYDKKLSPRVGSKPFITFIGEGFENVEELKHLKEVLLDMFRGEVVENINL 180

Query: 181 AGLDRVYVCTAISPNKVFLTHCALRLKKSGSVVPRMELVEVGPSMDMVVRRHRLPSDGLR 240
           AGLDRVYVCTAISPNKVFLTHCALR+KKSGSVVPRMELVEVGPSMD+VVRRHRLPSDGLR
Sbjct: 181 AGLDRVYVCTAISPNKVFLTHCALRMKKSGSVVPRMELVEVGPSMDLVVRRHRLPSDGLR 240

Query: 241 KEAMKTAKDQAKKKEKNVSQDAIQGKIGRIYIPDQKVHDMNSHIMFITKVGDMAIPDKSK 300
           KEAMKTAKDQAKKKEKNVSQDAI GKIGRIYIPDQ             KVGD+A+PDKSK
Sbjct: 241 KEAMKTAKDQAKKKEKNVSQDAIHGKIGRIYIPDQ-------------KVGDVALPDKSK 300

Query: 301 GVKRERREAKMKNKDDGPASKKPKEDSEKPKENSE 336
           GVKRER EAKMKN+D+GPASKK KEDSEKPKE+SE
Sbjct: 301 GVKRERCEAKMKNRDNGPASKKQKEDSEKPKEDSE 322

[Close]

BLAST of CmoCh14G002680.1 vs. ExPASy TrEMBL
Match: A0A0A0LAR1 (Ribosome production factor 2 homolog OS=Cucumis sativus OX=3659 GN=Csa_3G750370 PE=3 SV=1)

HSP 1 Score: 572.4 bits (1474), Expect = 1.2e-159
Identity = 297/335 (88.66%), Postives = 313/335 (93.43%), Query Frame = 0


Query: 1   MLKIRTPKNGKVRRELEKRAPKIVETGKKTLILHGTKTSGVLNAVLTELYHLKKESSVKY 60
           ML+IRTPKN KV+RELEKRAPK+VETGKKTLILHGTKTSGVLN VLTELYHLKKES+VKY
Sbjct: 1   MLQIRTPKNRKVKRELEKRAPKLVETGKKTLILHGTKTSGVLNEVLTELYHLKKESAVKY 60

Query: 61  SRKNDGIKPFESGGDSSLEFFSLKTDCSIIVYGSHSKKRPDNLVIGRTYDHKIYDLVEVG 120
           SRKNDGIKPFESGGD+SLEFFSLKTDCSII+YGSHSKKRP+NLVI RTY+  IYD+VEVG
Sbjct: 61  SRKNDGIKPFESGGDASLEFFSLKTDCSIILYGSHSKKRPNNLVIARTYNQHIYDMVEVG 120

Query: 121 IENFKSMHSFTYDKKLSPRVGSKPFITFIGEGFENVEELKHLKEVLLDLFRGEVVENLNL 180
           IENFKSMHSF YDKKLSPRVGSKPFITFIGEGFENVEELKHLKEVLLD FRGEVVENLNL
Sbjct: 121 IENFKSMHSFAYDKKLSPRVGSKPFITFIGEGFENVEELKHLKEVLLDFFRGEVVENLNL 180

Query: 181 AGLDRVYVCTAISPNKVFLTHCALRLKKSGSVVPRMELVEVGPSMDMVVRRHRLPSDGLR 240
           +GLDRVYVCTAISPNKVFLTHCALRLKKSGSVVPRMELVEVGPSMDMV+RRHRLP+DGLR
Sbjct: 181 SGLDRVYVCTAISPNKVFLTHCALRLKKSGSVVPRMELVEVGPSMDMVIRRHRLPTDGLR 240

Query: 241 KEAMKTAKDQAKKKEKNVSQDAIQGKIGRIYIPDQKVHDMNSHIMFITKVGDMAIPDKSK 300
           KEAMKTAKD+AKKKEKNVS+D IQGKIGRIYIPDQ             KVGDMAI DKSK
Sbjct: 241 KEAMKTAKDKAKKKEKNVSKDIIQGKIGRIYIPDQ-------------KVGDMAIVDKSK 300

Query: 301 GVKRERREAKMKNKDDGPASKKPKEDSEKPKENSE 336
           GVKRERREAKMKN+DDGPASKKPKEDSEKPKE+SE
Sbjct: 301 GVKRERREAKMKNRDDGPASKKPKEDSEKPKEDSE 322

[Close]

BLAST of CmoCh14G002680.1 vs. TAIR 10
Match: AT3G23620.1 (Ribosomal RNA processing Brix domain protein )

HSP 1 Score: 454.5 bits (1168), Expect = 7.3e-128
Identity = 227/328 (69.21%), Postives = 276/328 (84.15%), Query Frame = 0


Query: 1   MLKIRTPKNGKVRRELEKRAPKIVETGKKTLILHGTKTSGVLNAVLTELYHLKKESSVKY 60
           M++IRTPK GK +R LE RAPK+VETGKKTLILHGTKTS  L++V+TELY LKK  +++Y
Sbjct: 1   MMEIRTPKTGKAKRVLESRAPKLVETGKKTLILHGTKTSATLSSVMTELYRLKKGGAIRY 60

Query: 61  SRKNDGIKPFESGGDSSLEFFSLKTDCSIIVYGSHSKKRPDNLVIGRTYDHKIYDLVEVG 120
           SR+N+ I+PFESGG++SLEFFS KTDCSI VYGSH+KKRPDNLV+GR YDH++YDL+EVG
Sbjct: 61  SRRNENIRPFESGGETSLEFFSQKTDCSIFVYGSHTKKRPDNLVLGRMYDHQVYDLIEVG 120

Query: 121 IENFKSMHSFTYDKKLSPRVGSKPFITFIGEGFENVEELKHLKEVLLDLFRGEVVENLNL 180
           IENFKS+ +F+YDKK +P  G+KPFI FIGEGFENV ELKHLKEVL DLFRGEVV+NLNL
Sbjct: 121 IENFKSLRAFSYDKKFAPHEGTKPFICFIGEGFENVSELKHLKEVLTDLFRGEVVDNLNL 180

Query: 181 AGLDRVYVCTAISPNKVFLTHCALRLKKSGSVVPRMELVEVGPSMDMVVRRHRLPSDGLR 240
            GLDR YVC+AISP KVFLTHCAL+LKKSGS+VPRMELVEVGPSMD+V+RR+RLP+D L 
Sbjct: 181 TGLDRAYVCSAISPTKVFLTHCALKLKKSGSIVPRMELVEVGPSMDLVIRRNRLPNDSLM 240

Query: 241 KEAMKTAKDQAKKKEKNVSQDAIQGKIGRIYIPDQKVHDMNSHIMFITKVGDMAIPDKSK 300
           KEAM+T+KD+ KKKEKNV QDA+ GK G+IY+PDQK+ +M              + DKSK
Sbjct: 241 KEAMRTSKDKPKKKEKNVDQDAVLGKTGKIYMPDQKLKEMK-------------LFDKSK 300

Query: 301 GVKRERREAKMKNKDDGPASKKPKEDSE 329
           G KRER++AK+K+K++   +KK K  SE
Sbjct: 301 GSKRERKDAKLKHKEE-TVAKKMKVSSE 314

The following BLAST results are available for this feature:

BLAST of CmoCh14G002680.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LUG5	1.0e-126	69.21	Ribosome production factor 2 homolog OS=Arabidopsis thaliana OX=3702 GN=At3g2362...	[more]
Q9AWM9	1.3e-118	67.69	Ribosome production factor 2 homolog OS=Oryza sativa subsp. japonica OX=39947 GN...	[more]
Q9JJ80	1.8e-54	41.05	Ribosome production factor 2 homolog OS=Mus musculus OX=10090 GN=Rpf2 PE=2 SV=2	[more]
Q2YDN6	3.4e-53	39.81	Ribosome production factor 2 homolog OS=Bos taurus OX=9913 GN=RPF2 PE=2 SV=1	[more]
Q9H7B2	5.7e-53	40.12	Ribosome production factor 2 homolog OS=Homo sapiens OX=9606 GN=RPF2 PE=1 SV=2	[more]

BLAST of CmoCh14G002680.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F357	2.9e-172	96.12	Ribosome production factor 2 homolog OS=Cucurbita moschata OX=3662 GN=LOC1114419...	[more]
A0A6J1J655	1.8e-171	95.22	Ribosome production factor 2 homolog OS=Cucurbita maxima OX=3661 GN=LOC111481697...	[more]
A0A6J1KZU6	4.1e-163	89.52	Ribosome production factor 2 homolog OS=Cucurbita maxima OX=3661 GN=LOC111498582...	[more]
A0A6J1FZZ6	3.5e-162	89.25	Ribosome production factor 2 homolog OS=Cucurbita moschata OX=3662 GN=LOC1114494...	[more]
A0A0A0LAR1	1.2e-159	88.66	Ribosome production factor 2 homolog OS=Cucumis sativus OX=3659 GN=Csa_3G750370 ...	[more]

BLAST of CmoCh14G002680.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G23620.1	7.3e-128	69.21	Ribosomal RNA processing Brix domain protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007109	Brix domain	SMART	SM00879	Brix_2	coord: 31..232 e-value: 1.6E-36 score: 137.3
IPR007109	Brix domain	PFAM	PF04427	Brix	coord: 35..231 e-value: 2.9E-32 score: 112.4
IPR007109	Brix domain	PROSITE	PS50833	BRIX	coord: 28..238 score: 23.343094
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 296..335
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 295..335
IPR039770	Ribosome biogenesis protein Rpf2	PANTHER	PTHR12728	BRIX DOMAIN CONTAINING PROTEIN	coord: 1..324

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmoCh14G002680	CmoCh14G002680	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh14G002680.1:exon:4925	CmoCh14G002680.1:exon:4925	exon
CmoCh14G002680.1:exon:4924	CmoCh14G002680.1:exon:4924	exon
CmoCh14G002680.1:exon:4923	CmoCh14G002680.1:exon:4923	exon
CmoCh14G002680.1:exon:4922	CmoCh14G002680.1:exon:4922	exon
CmoCh14G002680.1:exon:4921	CmoCh14G002680.1:exon:4921	exon
CmoCh14G002680.1:exon:4920	CmoCh14G002680.1:exon:4920	exon
CmoCh14G002680.1:exon:4919	CmoCh14G002680.1:exon:4919	exon

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh14G002680.1:three_prime_utr	CmoCh14G002680.1:three_prime_utr	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh14G002680.1:cds	CmoCh14G002680.1:cds_7	CDS
CmoCh14G002680.1:cds	CmoCh14G002680.1:cds_6	CDS
CmoCh14G002680.1:cds	CmoCh14G002680.1:cds_5	CDS
CmoCh14G002680.1:cds	CmoCh14G002680.1:cds_4	CDS
CmoCh14G002680.1:cds	CmoCh14G002680.1:cds_3	CDS
CmoCh14G002680.1:cds	CmoCh14G002680.1:cds_2	CDS
CmoCh14G002680.1:cds	CmoCh14G002680.1:cds	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh14G002680.1:five_prime_utr	CmoCh14G002680.1:five_prime_utr	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmoCh14G002680.1	CmoCh14G002680.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000470	maturation of LSU-rRNA
biological_process	GO:0000027	ribosomal large subunit assembly
biological_process	GO:0006364	rRNA processing
biological_process	GO:0000463	maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
biological_process	GO:0032774	RNA biosynthetic process
cellular_component	GO:0005730	nucleolus
molecular_function	GO:0019843	rRNA binding