CmoCh11G019510.1 (mRNA) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh11G019510.1
Type	mRNA
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	E3 ubiquitin-protein ligase RMA1H1-like
Location	Cmo_Chr11: 13451684 .. 13453386 (-)
Sequence length	982
RNA-Seq Expression	CmoCh11G019510.1
Synteny	CmoCh11G019510.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGGGACCGCTCTATGGCTTGGAAATGAAGTTAGTTTCCTGTTTTCGTTATGAAATTCTAAAAACTTTTCATCGGAAGAAGATTTGATAACTCATAGTTCTAACTGCATAAAGATTCAGCGTTCTATCTGCATGTTTACTTGATTTTCTATGTGACTGGTCTTTGAAATCCATTTCAGATTGCTAGAAATGTTCTAGAAATCTGGATTTAGATATGGATTTGCATGTTTTAGAAGTGATAGTTTAGTTTGGAGGTCGAATCTTTATTTAATAGAGAGGAAATTGCCAATTCGGTGAAGAATTCTAATCCAAATGTCATTATCTGCCGACTCTTCCATGTTTGGTTTTTTTGTTTTCTATTGATTAACGTGCGGAATTACTCAAGATTTATTTATGTTAGATCTGAATATGGATAGTTTAAATCCAGAAACATTTCTAGAAGGTCAACTCCCAAATAGAATTTGAATTTTTTGGATTGGCTCTTGCTTTAAGTATGCAGGCGGAGGCATTAACCATGGGTTTGTCTTTATAATATTGTCAAGAGCTCTTATCTCCTTGGCCCTATCTTTCCTTGGGGTTGAAGGTATTCATTGTTGTAAAAAGAGCCAGAACAAGTTCATCTTCTTCATGTACATATGAACTTACTTGTTCTTGTTGTCCTGTTCTATTTATATCATCTCTATTTCTTCAAATTTGCATTAGTTTTGTGGGTTTTGATCCAATATTGATCTGAAATCTTATGATTTGGCAGTTTTGGTATCTCAGAAGGGTTCTTTACTACAGAGTATATGGACATGGAGCAGTACTATGAGGAGATTAATTCATTTGGAGAAAACAAGTCTTCCGTGGAGAATTGGAAATCAGCTTTGGATGCCATGGCAGAAACTCAATGTGATTCCTCTGGGCTATTTGACTGTAACATATGCTTAGAGACTGTGAAAGATCCTGTTGTTACACTTTGTGGCCATCTCTTTTGCTGGCCCTGTATTTACAAATGGCTTCATTTTCAGGACAGCTCTACAGAAAAACAAGGCCAACGACTCCAGCAATGTCCGGTTTGTAAGGCGGAAGTTTCTGATGCTACGCTAGTCCCGCTCTATGGCAAAGGTGAAACTCAAGATCCATTCGAAAGCAAGAATTCGCAGCTGGGAATCGTCGTCCCTCGTAGGCCTCAAGGACCTGCTTGCTTTGAGTCACCAAGGGCAACCTCTCACCCTCACCCTCACCCTCACCCTCACCCTGTTCTTCAATTCCAGCAAGGAAATTTATCAGATCAGTCGAACATTTACTATGCTGAAGGAGTATTCGGAGAAATGGTTTATGCGAGAATGTTCGGTGCGATAACGAATCTATATTCATATCCGAACTCGTATCCTCTTGTGTGGAGCAGTAGTCCTAGGATCAGAAGGCATATTTTGCAGACGGATGAATCACTCAATAGAATCTGCATCTTCCTTTTCTGTTGCTCAATTATCTGTCTTCTGTTGTTCTGAGGAGTAGGTTTTTTGTTTTAGTCTCCGACCCATTGTAAAAGAATCGTAGTTCGAACACTGCATGATTGCAGATTGAGTCTGATGTAGGTGTGGCTGAGTGTTGGTGGCGATGTTGCCCCTTGCCTGCCCTTCTTTTGCAGTTGATGTAAATAATAATTTTGCTTAACAGCCTCATTTTCATGGATTCTATCGCATTCTTGACTCTTGG

mRNA sequence

ATGAAGGGACCGCTCTATGGCTTGGAAATGAATTTTGGTATCTCAGAAGGGTTCTTTACTACAGAGTATATGGACATGGAGCAGTACTATGAGGAGATTAATTCATTTGGAGAAAACAAGTCTTCCGTGGAGAATTGGAAATCAGCTTTGGATGCCATGGCAGAAACTCAATGTGATTCCTCTGGGCTATTTGACTGTAACATATGCTTAGAGACTGTGAAAGATCCTGTTGTTACACTTTGTGGCCATCTCTTTTGCTGGCCCTGTATTTACAAATGGCTTCATTTTCAGGACAGCTCTACAGAAAAACAAGGCCAACGACTCCAGCAATGTCCGGTTTGTAAGGCGGAAGTTTCTGATGCTACGCTAGTCCCGCTCTATGGCAAAGGTGAAACTCAAGATCCATTCGAAAGCAAGAATTCGCAGCTGGGAATCGTCGTCCCTCGTAGGCCTCAAGGACCTGCTTGCTTTGAGTCACCAAGGGCAACCTCTCACCCTCACCCTCACCCTCACCCTCACCCTGTTCTTCAATTCCAGCAAGGAAATTTATCAGATCAGTCGAACATTTACTATGCTGAAGGAGTATTCGGAGAAATGGTTTATGCGAGAATGTTCGGTGCGATAACGAATCTATATTCATATCCGAACTCGTATCCTCTTGTGTGGAGCAGTAGTCCTAGGATCAGAAGGCATATTTTGCAGACGGATGAATCACTCAATAGAATCTGCATCTTCCTTTTCTGTTGCTCAATTATCTGTCTTCTGTTGTTCTGAGGAGTAGGTTTTTTGTTTTAGTCTCCGACCCATTGTAAAAGAATCGTAGTTCGAACACTGCATGATTGCAGATTGAGTCTGATGTAGGTGTGGCTGAGTGTTGGTGGCGATGTTGCCCCTTGCCTGCCCTTCTTTTGCAGTTGATGTAAATAATAATTTTGCTTAACAGCCTCATTTTCATGGATTCTATCGCATTCTTGACTCTTGG

Coding sequence (CDS)

ATGAAGGGACCGCTCTATGGCTTGGAAATGAATTTTGGTATCTCAGAAGGGTTCTTTACTACAGAGTATATGGACATGGAGCAGTACTATGAGGAGATTAATTCATTTGGAGAAAACAAGTCTTCCGTGGAGAATTGGAAATCAGCTTTGGATGCCATGGCAGAAACTCAATGTGATTCCTCTGGGCTATTTGACTGTAACATATGCTTAGAGACTGTGAAAGATCCTGTTGTTACACTTTGTGGCCATCTCTTTTGCTGGCCCTGTATTTACAAATGGCTTCATTTTCAGGACAGCTCTACAGAAAAACAAGGCCAACGACTCCAGCAATGTCCGGTTTGTAAGGCGGAAGTTTCTGATGCTACGCTAGTCCCGCTCTATGGCAAAGGTGAAACTCAAGATCCATTCGAAAGCAAGAATTCGCAGCTGGGAATCGTCGTCCCTCGTAGGCCTCAAGGACCTGCTTGCTTTGAGTCACCAAGGGCAACCTCTCACCCTCACCCTCACCCTCACCCTCACCCTGTTCTTCAATTCCAGCAAGGAAATTTATCAGATCAGTCGAACATTTACTATGCTGAAGGAGTATTCGGAGAAATGGTTTATGCGAGAATGTTCGGTGCGATAACGAATCTATATTCATATCCGAACTCGTATCCTCTTGTGTGGAGCAGTAGTCCTAGGATCAGAAGGCATATTTTGCAGACGGATGAATCACTCAATAGAATCTGCATCTTCCTTTTCTGTTGCTCAATTATCTGTCTTCTGTTGTTCTGA

Protein sequence

MKGPLYGLEMNFGISEGFFTTEYMDMEQYYEEINSFGENKSSVENWKSALDAMAETQCDSSGLFDCNICLETVKDPVVTLCGHLFCWPCIYKWLHFQDSSTEKQGQRLQQCPVCKAEVSDATLVPLYGKGETQDPFESKNSQLGIVVPRRPQGPACFESPRATSHPHPHPHPHPVLQFQQGNLSDQSNIYYAEGVFGEMVYARMFGAITNLYSYPNSYPLVWSSSPRIRRHILQTDESLNRICIFLFCCSIICLLLF

Homology

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BLAST of CmoCh11G019510.1 vs. ExPASy Swiss-Prot
Match: Q6R567 (E3 ubiquitin-protein ligase RMA1H1 OS=Capsicum annuum OX=4072 GN=RMA1H1 PE=1 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 3.4e-53
Identity = 113/255 (44.31%), Postives = 152/255 (59.61%), Query Frame = 0


Query: 24  MDMEQYYEEIN-SFGENKSSVENWKSALDAMAETQCDSSGLFDCNICLETVKDPVVTLCG 83
           M+ +   E+++ +F ++ + +  WKS  D + E   + SG FDCNICL+ V +PV+TLCG
Sbjct: 1   MNQDMALEQLDTTFNKHDTPLGKWKSMNDEVEE---NISGGFDCNICLDCVHEPVITLCG 60

Query: 84  HLFCWPCIYKWLHFQDSSTEKQGQRLQQCPVCKAEVSDATLVPLYGK-GETQDPFESKNS 143
           HL+CWPCIYKW++FQ  S+E   Q+  QCPVCKAEVS+ TL+PLYG+ G++  P E K  
Sbjct: 61  HLYCWPCIYKWIYFQSVSSENSDQQQPQCPVCKAEVSEKTLIPLYGRGGQSTKPSEGKAP 120

Query: 144 QLGIVVPRRPQGPAC---FESPRATSHPHPHPHPHPVLQFQQGNLSDQSNIYYA------ 203
            LGIV+P+RP  P C   F  P   S+P          Q  Q         Y +      
Sbjct: 121 NLGIVIPQRPPSPRCGGHFLLPTTDSNPSQLLQRRGYQQQSQTRQPAYQGSYMSSPMLSP 180

Query: 204 ---------EGVFGEMVYARMFG-AITNLYSYPNSYPLVWSSSPRIRRHILQTDESLNRI 258
                      + GE+ YAR+FG + T +Y+YPNSY L  SSSPR+RR + Q D SL RI
Sbjct: 181 GGATANMLQHSMIGEVAYARIFGNSSTTMYTYPNSYNLAISSSPRMRRQLSQADRSLGRI 240

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BLAST of CmoCh11G019510.1 vs. ExPASy Swiss-Prot
Match: O64425 (E3 ubiquitin-protein ligase RMA1 OS=Arabidopsis thaliana OX=3702 GN=RMA1 PE=1 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 7.0e-43
Identity = 98/209 (46.89%), Postives = 128/209 (61.24%), Query Frame = 0


Query: 64  FDCNICLETVKDPVVTLCGHLFCWPCIYKWLHFQDSSTEKQGQRLQQCPVCKAEVSDATL 123
           FDCNICL++V++PVVTLCGHLFCWPCI+KWL  Q  ST  + QR +QCPVCK++VS +TL
Sbjct: 46  FDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRHRQCPVCKSKVSHSTL 105

Query: 124 VPLYGKGETQDPFESKNSQLGIVVPRRPQGPAC-FESP---------RATSHPH---PHP 183
           VPLYG+G      E KNS     VP+RP GP    E P         R +   H   P  
Sbjct: 106 VPLYGRGRCTTQEEGKNS-----VPKRPVGPVYRLEMPNSPYASTDLRLSQRVHFNSPQE 165

Query: 184 HPHPVLQFQQGN-LSDQSNIYYAEGVFGEMVYARMFGA-ITNLYSYPNSYPLVWSSSPRI 243
             +PV      N LS  + +     + GEMV  R+FG  + + ++YP++Y L  +S PR+
Sbjct: 166 GYYPVSGVMSSNSLSYSAVLDPVMVMVGEMVATRLFGTRVMDRFAYPDTYNLAGTSGPRM 225

Query: 244 RRHILQTDESLNRICIFLFCCSIICLLLF 258
           RR I+Q D+SL RI  F  CC ++CLLLF
Sbjct: 226 RRRIMQADKSLGRIFFFFMCCVVLCLLLF 249

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BLAST of CmoCh11G019510.1 vs. ExPASy Swiss-Prot
Match: Q8GUK7 (E3 ubiquitin-protein ligase RMA3 OS=Arabidopsis thaliana OX=3702 GN=RMA3 PE=1 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 3.1e-38
Identity = 92/222 (41.44%), Postives = 118/222 (53.15%), Query Frame = 0


Query: 57  QCDSSGLFDCNICLETVKDPVVTLCGHLFCWPCIYKWLHFQDSSTEKQGQRLQQCPVCKA 116
           Q + SG FDCNICL+T  DPVVTLCGHLFCWPCIYKWLH Q SS     Q    CPVCK+
Sbjct: 35  QANESGCFDCNICLDTAHDPVVTLCGHLFCWPCIYKWLHVQLSSVSVD-QHQNNCPVCKS 94

Query: 117 EVSDATLVPLYGKG--ETQDPFESKNSQ-LGIVVPRRPQGPACFESP------------R 176
            ++  +LVPLYG+G       F SK    L   +PRRP  P+   +P             
Sbjct: 95  NITITSLVPLYGRGMSSPSSTFGSKKQDALSTDIPRRP-APSALRNPITSASSLNPSLQH 154

Query: 177 ATSHPHPHPHPHPVLQFQQGNLSDQSN------IYYAEGVFGEMVYARMFGAITNLYSYP 236
            T  P  H H +    F     +D +N      +Y   G+FG++VY R+FG  TN  + P
Sbjct: 155 QTLSPSFHNHQYSPRGFTTTESTDLANAVMMSFLYPVIGMFGDLVYTRIFGTFTNTIAQP 214

Query: 237 NSYPLVWSSSPRIRRHILQTDESLNRICIFLFCCSIICLLLF 258
                         + ++Q ++SLNR+ IF  CC I+CLLLF
Sbjct: 215 YQ-----------SQRMMQREKSLNRVSIFFLCCIILCLLLF 243

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BLAST of CmoCh11G019510.1 vs. ExPASy Swiss-Prot
Match: P93030 (E3 ubiquitin-protein ligase RMA2 OS=Arabidopsis thaliana OX=3702 GN=RMA2 PE=1 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.5e-32
Identity = 86/214 (40.19%), Postives = 116/214 (54.21%), Query Frame = 0


Query: 56  TQCDSSGLFDCNICLETVKDPVVTLCGHLFCWPCIYKWLHFQDSSTEKQGQ-----RLQQ 115
           T  DS G FDCNICL+ V+DPVVTLCGHLFCWPCI+KW +  ++S ++  Q        +
Sbjct: 11  TLVDSGGDFDCNICLDQVRDPVVTLCGHLFCWPCIHKWTYASNNSRQRVDQYDHKREPPK 70

Query: 116 CPVCKAEVSDATLVPLYGKGETQDPFESKNSQLGIVVPRRPQGPAC------FESPRATS 175
           CPVCK++VS+ATLVP+YG+G+       K  Q G  VP RP GP               S
Sbjct: 71  CPVCKSDVSEATLVPIYGRGQ-------KAPQSGSNVPSRPTGPVYDLRGVGQRLGEGES 130

Query: 176 HPHPHPHPHPVLQFQQGNLSDQSNIYYAEGVFGEMVYARMFG-AITNLYSYPNSYPLVWS 235
             + +  P PV+                 GV  EMVY R+FG + +N+  Y +       
Sbjct: 131 QRYMYRMPDPVM-----------------GVVCEMVYRRLFGESSSNMAPYRD------- 190

Query: 236 SSPRIRRHILQTDESLNRICIFLFCCSIICLLLF 258
            + R RR  +Q +ESL+R+ +FL C   +CL LF
Sbjct: 191 MNVRSRRRAMQAEESLSRVYLFLLCFMFMCLFLF 193

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BLAST of CmoCh11G019510.1 vs. ExPASy Swiss-Prot
Match: Q5ZIR9 (E3 ubiquitin-protein ligase RNF185 OS=Gallus gallus OX=9031 GN=RNF185 PE=2 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 5.4e-19
Identity = 45/92 (48.91%), Postives = 58/92 (63.04%), Query Frame = 0


Query: 64  FDCNICLETVKDPVVTLCGHLFCWPCIYKWLHFQDSSTEKQGQRLQQCPVCKAEVSDATL 123
           F+CNICL+T KD V++LCGHLFCWPC+++WL  + +         Q CPVCKA +S   +
Sbjct: 39  FECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNR--------QVCPVCKAGISRDKV 98

Query: 124 VPLYGKGET--QDPFESKNSQLGIVVPRRPQG 154
           +PLYG+G T  QDP E          P RPQG
Sbjct: 99  IPLYGRGSTGQQDPREK--------TPPRPQG 114

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BLAST of CmoCh11G019510.1 vs. ExPASy TrEMBL
Match: A0A6J1ERI0 (E3 ubiquitin-protein ligase RMA1H1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111437136 PE=4 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 6.6e-137
Identity = 234/234 (100.00%), Postives = 234/234 (100.00%), Query Frame = 0


Query: 24  MDMEQYYEEINSFGENKSSVENWKSALDAMAETQCDSSGLFDCNICLETVKDPVVTLCGH 83
           MDMEQYYEEINSFGENKSSVENWKSALDAMAETQCDSSGLFDCNICLETVKDPVVTLCGH
Sbjct: 1   MDMEQYYEEINSFGENKSSVENWKSALDAMAETQCDSSGLFDCNICLETVKDPVVTLCGH 60

Query: 84  LFCWPCIYKWLHFQDSSTEKQGQRLQQCPVCKAEVSDATLVPLYGKGETQDPFESKNSQL 143
           LFCWPCIYKWLHFQDSSTEKQGQRLQQCPVCKAEVSDATLVPLYGKGETQDPFESKNSQL
Sbjct: 61  LFCWPCIYKWLHFQDSSTEKQGQRLQQCPVCKAEVSDATLVPLYGKGETQDPFESKNSQL 120

Query: 144 GIVVPRRPQGPACFESPRATSHPHPHPHPHPVLQFQQGNLSDQSNIYYAEGVFGEMVYAR 203
           GIVVPRRPQGPACFESPRATSHPHPHPHPHPVLQFQQGNLSDQSNIYYAEGVFGEMVYAR
Sbjct: 121 GIVVPRRPQGPACFESPRATSHPHPHPHPHPVLQFQQGNLSDQSNIYYAEGVFGEMVYAR 180

Query: 204 MFGAITNLYSYPNSYPLVWSSSPRIRRHILQTDESLNRICIFLFCCSIICLLLF 258
           MFGAITNLYSYPNSYPLVWSSSPRIRRHILQTDESLNRICIFLFCCSIICLLLF
Sbjct: 181 MFGAITNLYSYPNSYPLVWSSSPRIRRHILQTDESLNRICIFLFCCSIICLLLF 234

[Close]

BLAST of CmoCh11G019510.1 vs. ExPASy TrEMBL
Match: A0A6J1JKZ9 (E3 ubiquitin-protein ligase RMA1H1-like OS=Cucurbita maxima OX=3661 GN=LOC111486145 PE=4 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 7.4e-128
Identity = 224/234 (95.73%), Postives = 226/234 (96.58%), Query Frame = 0


Query: 24  MDMEQYYEEINSFGENKSSVENWKSALDAMAETQCDSSGLFDCNICLETVKDPVVTLCGH 83
           MDMEQY+EEINSFGENKSSVENWKSALDAMAETQCDSSGLFDCNICLETVKDPVVTLCGH
Sbjct: 1   MDMEQYFEEINSFGENKSSVENWKSALDAMAETQCDSSGLFDCNICLETVKDPVVTLCGH 60

Query: 84  LFCWPCIYKWLHFQDSSTEKQGQRLQQCPVCKAEVSDATLVPLYGKGETQDPFESKNSQL 143
           LFCWPCIYKWLHFQDSSTEKQGQRLQQCPVCKAEVSDATLVPLYGKGETQDPFESKNSQL
Sbjct: 61  LFCWPCIYKWLHFQDSSTEKQGQRLQQCPVCKAEVSDATLVPLYGKGETQDPFESKNSQL 120

Query: 144 GIVVPRRPQGPACFESPRATSHPHPHPHPHPVLQFQQGNLSDQSNIYYAEGVFGEMVYAR 203
           GIVVPRRPQGPACFESPRATS        HPVLQFQQGNLSDQSNIYYAEGVFGEMVYAR
Sbjct: 121 GIVVPRRPQGPACFESPRATS--------HPVLQFQQGNLSDQSNIYYAEGVFGEMVYAR 180

Query: 204 MFGAITNLYSYPNSYPLVWSSSPRIRRHILQTDESLNRICIFLFCCSIICLLLF 258
           MFGAITNLY+YPNSYPLVWSSSPRIRRHILQTDESLNRICIFLFCCSIICLLLF
Sbjct: 181 MFGAITNLYAYPNSYPLVWSSSPRIRRHILQTDESLNRICIFLFCCSIICLLLF 226

[Close]

BLAST of CmoCh11G019510.1 vs. ExPASy TrEMBL
Match: A0A6J1D3G6 (E3 ubiquitin-protein ligase RMA1H1-like OS=Momordica charantia OX=3673 GN=LOC111016683 PE=4 SV=1)

HSP 1 Score: 404.1 bits (1037), Expect = 4.5e-109
Identity = 200/241 (82.99%), Postives = 212/241 (87.97%), Query Frame = 0


Query: 24  MDMEQYYEE----INSFGENKSSVENWKSALDAMAETQCDSSGLFDCNICLETVKDPVVT 83
           MDMEQY+EE    IN+FGENKSS+ENWKS LDAMAET CD+SGLFDCNICLETVKDPVVT
Sbjct: 1   MDMEQYFEEASLQINTFGENKSSLENWKSVLDAMAETHCDTSGLFDCNICLETVKDPVVT 60

Query: 84  LCGHLFCWPCIYKWLHFQDSSTEKQGQRLQQCPVCKAEVSDATLVPLYGKGETQDPFESK 143
           LCGHLFCWPCIYKWLHFQDSSTEKQGQ+ QQCPVCKAEVSDATLVPLYGKGETQDPFESK
Sbjct: 61  LCGHLFCWPCIYKWLHFQDSSTEKQGQQPQQCPVCKAEVSDATLVPLYGKGETQDPFESK 120

Query: 144 NSQLGIVVPRRPQGPACFESPRATSHPHPHPHPHPVLQFQQGNLSDQ-SNIYYAE--GVF 203
           NSQLG+VVPRRPQGP C E+ R TSH  P        QF++GN SDQ SN YYA+  GVF
Sbjct: 121 NSQLGVVVPRRPQGPGCSETSRPTSHSAP--------QFRRGNSSDQSSNNYYAQMTGVF 180

Query: 204 GEMVYARMFGAITNLYSYPNSYPLVWSSSPRIRRHILQTDESLNRICIFLFCCSIICLLL 258
           GEM+YARMFGAITN Y+YPNSYPLVWSSSPRIRRHIL TDESLNRICIFLFC SIICLLL
Sbjct: 181 GEMIYARMFGAITNHYAYPNSYPLVWSSSPRIRRHILATDESLNRICIFLFCFSIICLLL 233

[Close]

BLAST of CmoCh11G019510.1 vs. ExPASy TrEMBL
Match: A0A1S3C1W7 (E3 ubiquitin-protein ligase RMA1H1 OS=Cucumis melo OX=3656 GN=LOC103495518 PE=4 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 7.7e-109
Identity = 199/242 (82.23%), Postives = 213/242 (88.02%), Query Frame = 0


Query: 24  MDMEQYYEE----INSFGENKSSVENWKSALDAMAETQCDSSGLFDCNICLETVKDPVVT 83
           M+MEQY+EE    INSFGENKSS+ENWKS LDAMAET CD+SGLFDCNICLETVKDPVVT
Sbjct: 1   MEMEQYFEEASIQINSFGENKSSLENWKSVLDAMAETHCDASGLFDCNICLETVKDPVVT 60

Query: 84  LCGHLFCWPCIYKWLHFQDSSTEKQGQRLQQCPVCKAEVSDATLVPLYGKGETQDPFESK 143
           LCGHLFCWPCIYKW+HFQDSS EKQ +RL QCPVCKAEVSDATLVPLYGKGETQ+P ESK
Sbjct: 61  LCGHLFCWPCIYKWIHFQDSSLEKQARRLPQCPVCKAEVSDATLVPLYGKGETQNPLESK 120

Query: 144 NSQLGIVVPRRPQGPACFESPRATSHPHPHPHPHPVLQFQQG--NLSDQSNIYYAE--GV 203
           N QLGIVVPRRPQGPACFESPR TSHP  H       QF++G  + SDQSN+YYA+  G 
Sbjct: 121 NPQLGIVVPRRPQGPACFESPRPTSHPTSHTVGP---QFREGSSDSSDQSNVYYAQVTGA 180

Query: 204 FGEMVYARMFGAITNLYSYPNSYPLVWSSSPRIRRHILQTDESLNRICIFLFCCSIICLL 258
           FGE+VYARM GAITNLY+YPNSYPLVWSSSPRIRRHILQTDESLNRICIFLFCC IICLL
Sbjct: 181 FGEVVYARMSGAITNLYAYPNSYPLVWSSSPRIRRHILQTDESLNRICIFLFCCLIICLL 239

[Close]

BLAST of CmoCh11G019510.1 vs. ExPASy TrEMBL
Match: A0A5A7SLT9 (E3 ubiquitin-protein ligase RMA1H1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003470 PE=4 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 7.7e-109
Identity = 199/242 (82.23%), Postives = 213/242 (88.02%), Query Frame = 0


Query: 24  MDMEQYYEE----INSFGENKSSVENWKSALDAMAETQCDSSGLFDCNICLETVKDPVVT 83
           M+MEQY+EE    INSFGENKSS+ENWKS LDAMAET CD+SGLFDCNICLETVKDPVVT
Sbjct: 1   MEMEQYFEEASIQINSFGENKSSLENWKSVLDAMAETHCDASGLFDCNICLETVKDPVVT 60

Query: 84  LCGHLFCWPCIYKWLHFQDSSTEKQGQRLQQCPVCKAEVSDATLVPLYGKGETQDPFESK 143
           LCGHLFCWPCIYKW+HFQDSS EKQ +RL QCPVCKAEVSDATLVPLYGKGETQ+P ESK
Sbjct: 61  LCGHLFCWPCIYKWIHFQDSSLEKQARRLPQCPVCKAEVSDATLVPLYGKGETQNPLESK 120

Query: 144 NSQLGIVVPRRPQGPACFESPRATSHPHPHPHPHPVLQFQQG--NLSDQSNIYYAE--GV 203
           N QLGIVVPRRPQGPACFESPR TSHP  H       QF++G  + SDQSN+YYA+  G 
Sbjct: 121 NPQLGIVVPRRPQGPACFESPRPTSHPTSHTVGP---QFREGSSDSSDQSNVYYAQVTGA 180

Query: 204 FGEMVYARMFGAITNLYSYPNSYPLVWSSSPRIRRHILQTDESLNRICIFLFCCSIICLL 258
           FGE+VYARM GAITNLY+YPNSYPLVWSSSPRIRRHILQTDESLNRICIFLFCC IICLL
Sbjct: 181 FGEVVYARMSGAITNLYAYPNSYPLVWSSSPRIRRHILQTDESLNRICIFLFCCLIICLL 239

[Close]

BLAST of CmoCh11G019510.1 vs. TAIR 10
Match: AT4G03510.1 (RING membrane-anchor 1 )

HSP 1 Score: 175.6 bits (444), Expect = 5.0e-44
Identity = 98/209 (46.89%), Postives = 128/209 (61.24%), Query Frame = 0


Query: 64  FDCNICLETVKDPVVTLCGHLFCWPCIYKWLHFQDSSTEKQGQRLQQCPVCKAEVSDATL 123
           FDCNICL++V++PVVTLCGHLFCWPCI+KWL  Q  ST  + QR +QCPVCK++VS +TL
Sbjct: 46  FDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRHRQCPVCKSKVSHSTL 105

Query: 124 VPLYGKGETQDPFESKNSQLGIVVPRRPQGPAC-FESP---------RATSHPH---PHP 183
           VPLYG+G      E KNS     VP+RP GP    E P         R +   H   P  
Sbjct: 106 VPLYGRGRCTTQEEGKNS-----VPKRPVGPVYRLEMPNSPYASTDLRLSQRVHFNSPQE 165

Query: 184 HPHPVLQFQQGN-LSDQSNIYYAEGVFGEMVYARMFGA-ITNLYSYPNSYPLVWSSSPRI 243
             +PV      N LS  + +     + GEMV  R+FG  + + ++YP++Y L  +S PR+
Sbjct: 166 GYYPVSGVMSSNSLSYSAVLDPVMVMVGEMVATRLFGTRVMDRFAYPDTYNLAGTSGPRM 225

Query: 244 RRHILQTDESLNRICIFLFCCSIICLLLF 258
           RR I+Q D+SL RI  F  CC ++CLLLF
Sbjct: 226 RRRIMQADKSLGRIFFFFMCCVVLCLLLF 249

[Close]

BLAST of CmoCh11G019510.1 vs. TAIR 10
Match: AT4G03510.2 (RING membrane-anchor 1 )

HSP 1 Score: 175.6 bits (444), Expect = 5.0e-44
Identity = 98/209 (46.89%), Postives = 128/209 (61.24%), Query Frame = 0


Query: 64  FDCNICLETVKDPVVTLCGHLFCWPCIYKWLHFQDSSTEKQGQRLQQCPVCKAEVSDATL 123
           FDCNICL++V++PVVTLCGHLFCWPCI+KWL  Q  ST  + QR +QCPVCK++VS +TL
Sbjct: 46  FDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRHRQCPVCKSKVSHSTL 105

Query: 124 VPLYGKGETQDPFESKNSQLGIVVPRRPQGPAC-FESP---------RATSHPH---PHP 183
           VPLYG+G      E KNS     VP+RP GP    E P         R +   H   P  
Sbjct: 106 VPLYGRGRCTTQEEGKNS-----VPKRPVGPVYRLEMPNSPYASTDLRLSQRVHFNSPQE 165

Query: 184 HPHPVLQFQQGN-LSDQSNIYYAEGVFGEMVYARMFGA-ITNLYSYPNSYPLVWSSSPRI 243
             +PV      N LS  + +     + GEMV  R+FG  + + ++YP++Y L  +S PR+
Sbjct: 166 GYYPVSGVMSSNSLSYSAVLDPVMVMVGEMVATRLFGTRVMDRFAYPDTYNLAGTSGPRM 225

Query: 244 RRHILQTDESLNRICIFLFCCSIICLLLF 258
           RR I+Q D+SL RI  F  CC ++CLLLF
Sbjct: 226 RRRIMQADKSLGRIFFFFMCCVVLCLLLF 249

[Close]

BLAST of CmoCh11G019510.1 vs. TAIR 10
Match: AT4G27470.1 (RING membrane-anchor 3 )

HSP 1 Score: 160.2 bits (404), Expect = 2.2e-39
Identity = 92/222 (41.44%), Postives = 118/222 (53.15%), Query Frame = 0


Query: 57  QCDSSGLFDCNICLETVKDPVVTLCGHLFCWPCIYKWLHFQDSSTEKQGQRLQQCPVCKA 116
           Q + SG FDCNICL+T  DPVVTLCGHLFCWPCIYKWLH Q SS     Q    CPVCK+
Sbjct: 35  QANESGCFDCNICLDTAHDPVVTLCGHLFCWPCIYKWLHVQLSSVSVD-QHQNNCPVCKS 94

Query: 117 EVSDATLVPLYGKG--ETQDPFESKNSQ-LGIVVPRRPQGPACFESP------------R 176
            ++  +LVPLYG+G       F SK    L   +PRRP  P+   +P             
Sbjct: 95  NITITSLVPLYGRGMSSPSSTFGSKKQDALSTDIPRRP-APSALRNPITSASSLNPSLQH 154

Query: 177 ATSHPHPHPHPHPVLQFQQGNLSDQSN------IYYAEGVFGEMVYARMFGAITNLYSYP 236
            T  P  H H +    F     +D +N      +Y   G+FG++VY R+FG  TN  + P
Sbjct: 155 QTLSPSFHNHQYSPRGFTTTESTDLANAVMMSFLYPVIGMFGDLVYTRIFGTFTNTIAQP 214

Query: 237 NSYPLVWSSSPRIRRHILQTDESLNRICIFLFCCSIICLLLF 258
                         + ++Q ++SLNR+ IF  CC I+CLLLF
Sbjct: 215 YQ-----------SQRMMQREKSLNRVSIFFLCCIILCLLLF 243

[Close]

BLAST of CmoCh11G019510.1 vs. TAIR 10
Match: AT4G28270.1 (RING membrane-anchor 2 )

HSP 1 Score: 141.4 bits (355), Expect = 1.0e-33
Identity = 86/214 (40.19%), Postives = 116/214 (54.21%), Query Frame = 0


Query: 56  TQCDSSGLFDCNICLETVKDPVVTLCGHLFCWPCIYKWLHFQDSSTEKQGQ-----RLQQ 115
           T  DS G FDCNICL+ V+DPVVTLCGHLFCWPCI+KW +  ++S ++  Q        +
Sbjct: 11  TLVDSGGDFDCNICLDQVRDPVVTLCGHLFCWPCIHKWTYASNNSRQRVDQYDHKREPPK 70

Query: 116 CPVCKAEVSDATLVPLYGKGETQDPFESKNSQLGIVVPRRPQGPAC------FESPRATS 175
           CPVCK++VS+ATLVP+YG+G+       K  Q G  VP RP GP               S
Sbjct: 71  CPVCKSDVSEATLVPIYGRGQ-------KAPQSGSNVPSRPTGPVYDLRGVGQRLGEGES 130

Query: 176 HPHPHPHPHPVLQFQQGNLSDQSNIYYAEGVFGEMVYARMFG-AITNLYSYPNSYPLVWS 235
             + +  P PV+                 GV  EMVY R+FG + +N+  Y +       
Sbjct: 131 QRYMYRMPDPVM-----------------GVVCEMVYRRLFGESSSNMAPYRD------- 190

Query: 236 SSPRIRRHILQTDESLNRICIFLFCCSIICLLLF 258
            + R RR  +Q +ESL+R+ +FL C   +CL LF
Sbjct: 191 MNVRSRRRAMQAEESLSRVYLFLLCFMFMCLFLF 193

[Close]

BLAST of CmoCh11G019510.1 vs. TAIR 10
Match: AT1G74990.1 (RING/U-box superfamily protein )

HSP 1 Score: 115.5 bits (288), Expect = 6.1e-26
Identity = 53/104 (50.96%), Postives = 67/104 (64.42%), Query Frame = 0


Query: 49  ALDAMAETQCDSSGLFDCNICLETVKDPVVTLCGHLFCWPCIYKWLHFQDSSTEKQGQRL 108
           A++ +   + D+S  F CNICLE  ++P+VTLCGHLFCWPC+YKWLH+   S        
Sbjct: 2   AINTITNEEDDASNNFGCNICLELAREPIVTLCGHLFCWPCLYKWLHYHSKS-------- 61

Query: 109 QQCPVCKAEVSDATLVPLYGKGE-TQDPFESKNSQLGIVVPRRP 152
             CPVCKA V + TLVPLYG G+ + DP    NS  G+ VP RP
Sbjct: 62  NHCPVCKALVKEDTLVPLYGMGKPSSDPRSKLNS--GVTVPNRP 95

The following BLAST results are available for this feature:

BLAST of CmoCh11G019510.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6R567	3.4e-53	44.31	E3 ubiquitin-protein ligase RMA1H1 OS=Capsicum annuum OX=4072 GN=RMA1H1 PE=1 SV=...	[more]
O64425	7.0e-43	46.89	E3 ubiquitin-protein ligase RMA1 OS=Arabidopsis thaliana OX=3702 GN=RMA1 PE=1 SV...	[more]
Q8GUK7	3.1e-38	41.44	E3 ubiquitin-protein ligase RMA3 OS=Arabidopsis thaliana OX=3702 GN=RMA3 PE=1 SV...	[more]
P93030	1.5e-32	40.19	E3 ubiquitin-protein ligase RMA2 OS=Arabidopsis thaliana OX=3702 GN=RMA2 PE=1 SV...	[more]
Q5ZIR9	5.4e-19	48.91	E3 ubiquitin-protein ligase RNF185 OS=Gallus gallus OX=9031 GN=RNF185 PE=2 SV=1	[more]

BLAST of CmoCh11G019510.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ERI0	6.6e-137	100.00	E3 ubiquitin-protein ligase RMA1H1-like isoform X1 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1JKZ9	7.4e-128	95.73	E3 ubiquitin-protein ligase RMA1H1-like OS=Cucurbita maxima OX=3661 GN=LOC111486...	[more]
A0A6J1D3G6	4.5e-109	82.99	E3 ubiquitin-protein ligase RMA1H1-like OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A1S3C1W7	7.7e-109	82.23	E3 ubiquitin-protein ligase RMA1H1 OS=Cucumis melo OX=3656 GN=LOC103495518 PE=4 ...	[more]
A0A5A7SLT9	7.7e-109	82.23	E3 ubiquitin-protein ligase RMA1H1 OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]

BLAST of CmoCh11G019510.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G03510.1	5.0e-44	46.89	RING membrane-anchor 1	[more]
AT4G03510.2	5.0e-44	46.89	RING membrane-anchor 1	[more]
AT4G27470.1	2.2e-39	41.44	RING membrane-anchor 3	[more]
AT4G28270.1	1.0e-33	40.19	RING membrane-anchor 2	[more]
AT1G74990.1	6.1e-26	50.96	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 66..114 e-value: 1.1E-10 score: 51.5
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 66..115 score: 13.123657
IPR018957	Zinc finger, C3HC4 RING-type	PFAM	PF00097	zf-C3HC4	coord: 66..114 e-value: 7.7E-10 score: 38.5
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 23..130 e-value: 7.9E-19 score: 69.3
IPR045103	E3 ubiquitin-protein ligase RNF5/RNF185-like	PANTHER	PTHR12313	E3 UBIQUITIN-PROTEIN LIGASE RNF5-RELATED	coord: 27..257
None	No IPR available	PANTHER	PTHR12313:SF59	E3 UBIQUITIN-PROTEIN LIGASE RMA1	coord: 27..257
None	No IPR available	CDD	cd16745	RING-HC_AtRMA_like	coord: 64..116 e-value: 1.25248E-24 score: 90.8263
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 65..120
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 81..90

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmoCh11G019510	CmoCh11G019510	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh11G019510.1:exon:10180	CmoCh11G019510.1:exon:10180	exon
CmoCh11G019510.1:exon:10179	CmoCh11G019510.1:exon:10179	exon

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh11G019510.1:three_prime_utr	CmoCh11G019510.1:three_prime_utr	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh11G019510.1:cds	CmoCh11G019510.1:cds_2	CDS
CmoCh11G019510.1:cds	CmoCh11G019510.1:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmoCh11G019510.1	CmoCh11G019510.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0061630	ubiquitin protein ligase activity