CmoCh10G002170.1 (mRNA) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh10G002170.1
Type	mRNA
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Protein of unknown function (DUF962)
Location	Cmo_Chr10: 956719 .. 958027 (+)
Sequence length	840
RNA-Seq Expression	CmoCh10G002170.1
Synteny	CmoCh10G002170.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGAAGACTGGTTTGTTTGATCTGGAGAGGCATTTCGCCTTCTATGGCGCTTATCACAGCAACCCAGTCAACATTTTCATTCATGTTCTGTTTGTGTGGCCAATTTTCTTTACCACCCTCAGTATTTGTACTTCACCCCATCTTTCTACACTATTCCCAAATCCCCTTGTGGGTTTGACCGCGGCCTGGCCTTCAACTTCGGATTTCTTTTCACCTTAATATATGCTGCATCTTATGTGGTCTTCGATAAGAAAGCGGGGTCCAATGCCGCTTTGCCTTGCTTGATTTGTTGGGTTGGAGCAAGCATACTCGCCTTTAGACTTGGTTATTCTCAGACCTGGAAGGTAATTCCCTGCTTCCTCTCCAATTTAATTTATGGGTCCTTGGTTTTTGCAGTAAATCAATCTGTTTCCTATAGCTTACCCACTTCGATCTCTAATCCTCTTGTTGAGTGAATTGAATGGGATTAGTGGCAAGCGTTGGAAAACAGCTCAATCTGATATAATTTAGTTGGAGTTAGGTAGATGTGTTAAAGTTTGACATCTTGGTGGGAACAAGTTGGGGCCATCTCAAACGAGAGTTTGTTTGTCTAAAATTTTATTCATTTCTGTTGCCCATCAGATAGTACTGACTGCTCAGTTGTTCTGTTGGACCAATCAGTTAATAGACCATGGAGTATTTGAGGTTAGACAGGTTACTTTCATCTCAGTGAATTGATGAATCTCTTCATGATTCTTAAGCCATTTAATTTCATCTTTTGATAGAAACGAGCACCGGCTTTGTTAGACAATCTTGCTCAACCTTTTCTAATGGCTCCATTCTTTGTATTTTTTGAGGTATTTCTTCTCTGGTTATTCGGAGAGTTACTTGTCGGTGTTGATATTGAAGCAACTTTGTTGTTTCTGATGTTTGGTGTCAGGTTCTTCAAAGTTTGTTCAAATATGAACCATACCCAGGATTTAGTGCGAGTGTGCAAGCGAAGATAAAAGCAGATATCAAAGAGTGGAAAGAACAGAAGGAAAAGCTGACATAGGTTGAATTGCCTAAAGTTCAAGACAACTCTTGCCTAGATCTCCCTTTGTAGTAGTACGAAGAGAAAAACATTTTGAAATCTTTACGTGATATTTGTTAGGAATCACGAACTTTCACAATGATATGATATTGTCTATTTTGAGCATAAACTCTCATGAAGACATGACTCTGATACCATGTTAGAAATCACGTACCTCCGCACTAGTATGATATTGTCCACTGCTCTCATGGCATAAGCTCCCTCCCAACAATCCTCGACAATATACGATGTTTT

mRNA sequence

ATGGGGAAGACTGGTTTGTTTGATCTGGAGAGGCATTTCGCCTTCTATGGCGCTTATCACAGCAACCCAGTCAACATTTTCATTCATTATTTGTACTTCACCCCATCTTTCTACACTATTCCCAAATCCCCTTGTGGGTTTGACCGCGGCCTGGCCTTCAACTTCGGATTTCTTTTCACCTTAATATATGCTGCATCTTATGTGGTCTTCGATAAGAAAGCGGGGTCCAATGCCGCTTTGCCTTGCTTGATTTGTTGGGTTGGAGCAAGCATACTCGCCTTTAGACTTGGTTATTCTCAGACCTGGAAGATAGTACTGACTGCTCAGTTGTTCTGTTGGACCAATCAGTTAATAGACCATGGAGTATTTGAGGTTAGACAGAAACGAGCACCGGCTTTGTTAGACAATCTTGCTCAACCTTTTCTAATGGCTCCATTCTTTGTATTTTTTGAGGTTCTTCAAAGTTTGTTCAAATATGAACCATACCCAGGATTTAGTGCGAGTGTGCAAGCGAAGATAAAAGCAGATATCAAAGAGTGGAAAGAACAGAAGGAAAAGCTGACATAGGTTGAATTGCCTAAAGTTCAAGACAACTCTTGCCTAGATCTCCCTTTGTAGTAGTACGAAGAGAAAAACATTTTGAAATCTTTACGTGATATTTGTTAGGAATCACGAACTTTCACAATGATATGATATTGTCTATTTTGAGCATAAACTCTCATGAAGACATGACTCTGATACCATGTTAGAAATCACGTACCTCCGCACTAGTATGATATTGTCCACTGCTCTCATGGCATAAGCTCCCTCCCAACAATCCTCGACAATATACGATGTTTT

Coding sequence (CDS)

ATGGGGAAGACTGGTTTGTTTGATCTGGAGAGGCATTTCGCCTTCTATGGCGCTTATCACAGCAACCCAGTCAACATTTTCATTCATTATTTGTACTTCACCCCATCTTTCTACACTATTCCCAAATCCCCTTGTGGGTTTGACCGCGGCCTGGCCTTCAACTTCGGATTTCTTTTCACCTTAATATATGCTGCATCTTATGTGGTCTTCGATAAGAAAGCGGGGTCCAATGCCGCTTTGCCTTGCTTGATTTGTTGGGTTGGAGCAAGCATACTCGCCTTTAGACTTGGTTATTCTCAGACCTGGAAGATAGTACTGACTGCTCAGTTGTTCTGTTGGACCAATCAGTTAATAGACCATGGAGTATTTGAGGTTAGACAGAAACGAGCACCGGCTTTGTTAGACAATCTTGCTCAACCTTTTCTAATGGCTCCATTCTTTGTATTTTTTGAGGTTCTTCAAAGTTTGTTCAAATATGAACCATACCCAGGATTTAGTGCGAGTGTGCAAGCGAAGATAAAAGCAGATATCAAAGAGTGGAAAGAACAGAAGGAAAAGCTGACATAG

Protein sequence

MGKTGLFDLERHFAFYGAYHSNPVNIFIHYLYFTPSFYTIPKSPCGFDRGLAFNFGFLFTLIYAASYVVFDKKAGSNAALPCLICWVGASILAFRLGYSQTWKIVLTAQLFCWTNQLIDHGVFEVRQKRAPALLDNLAQPFLMAPFFVFFEVLQSLFKYEPYPGFSASVQAKIKADIKEWKEQKEKLT

Homology

[Close]

BLAST of CmoCh10G002170.1 vs. ExPASy Swiss-Prot
Match: O13737 (2-hydroxy-palmitic acid dioxygenase mpo1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mpo1 PE=3 SV=2)

HSP 1 Score: 81.6 bits (200), Expect = 1.0e-14
Identity = 63/176 (35.80%), Postives = 79/176 (44.89%), Query Frame = 0


Query: 9   LERHFAFYGAYHSNPVNIFIHYLYFTPSFYTIPKSPCGF-----DRGLAFNFGFLFTLIY 68
           L R ++FY AYHSNPVNI IH +       T       F     +  L  N   L  L Y
Sbjct: 4   LSRSYSFYAAYHSNPVNIKIHQVCIPLLLLTALVLLHNFVITLINSKLQINVAHLVGLAY 63

Query: 69  AASYVVFDKKAG-SNAALPCLICWVGASILAFRLGYSQTWKIVLTAQLFCWTNQLIDHGV 128
              YV  D   G   + +  L  ++  S L      S   +      + CW  Q I HGV
Sbjct: 64  QIFYVTLDPLDGLLYSPVLYLFSYILPSKLFTIFSRSLVNRSAAVVHVICWILQFIGHGV 123

Query: 129 FEVRQKRAPALLDNLAQPFLMAPFFVFFEVLQSLFKYEPYPGFSASVQAKIKADIK 179
           FE   KR PALLDNL Q   +AP F F E         P+ G+  SV +KI+A+IK
Sbjct: 124 FE---KRKPALLDNLIQSLFIAPLFAFLET-------GPFVGYYPSVVSKIRANIK 169

[Close]

BLAST of CmoCh10G002170.1 vs. ExPASy Swiss-Prot
Match: P25338 (2-hydroxy-palmitic acid dioxygenase MPO1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=MPO1 PE=1 SV=2)

HSP 1 Score: 70.1 bits (170), Expect = 3.0e-11
Identity = 54/181 (29.83%), Postives = 81/181 (44.75%), Query Frame = 0


Query: 5   GLFDLERHFAFYGAYHSNPVNIFIHYLYFTPSFYTIPKSPCGFDR-------GLAFNFGF 64
           GL DL     FY  YH NP N+ IH ++     ++     C   R        L      
Sbjct: 4   GLLDLRSQLGFYKFYHHNPKNVLIHSIFVPTILFS---GSCMLHRVKIYQSISLTAVLSV 63

Query: 65  LFTLIYAASYVVFDKKAGSNAALPCLICWVGASILAFRLGYSQTWKIVLTAQLFCWTNQL 124
           LF++ Y   Y+     AG       L+  +  +++  R+    T+K  L      W  Q 
Sbjct: 64  LFSIFYCLLYLPTGLLAG------VLLLLLNLALIDHRV--DLTFKQELGLFTIGWIFQF 123

Query: 125 IDHGVFEVRQKRAPALLDNLAQPFLMAPFFVFFEVLQSLFKYEPYPGFSASVQAKIKADI 179
           + HGVFE   KR PAL+DNL Q  ++AP+F+ FE L  L       GF   ++A ++ D+
Sbjct: 124 VGHGVFE---KRRPALIDNLVQSLVLAPYFIMFEFLFKL-------GFMPRLKATLEHDL 163

[Close]

BLAST of CmoCh10G002170.1 vs. ExPASy TrEMBL
Match: A0A6J1F4N0 (uncharacterized endoplasmic reticulum membrane protein C16E8.02 OS=Cucurbita moschata OX=3662 GN=LOC111440831 PE=4 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 1.5e-85
Identity = 160/201 (79.60%), Postives = 170/201 (84.58%), Query Frame = 0


Query: 1   MGKTGLFDLERHFAFYGAYHSNPVNIFIH-------------YLYFTPSFYTIPKSPCGF 60
           MGKT  FDLERHFAFYGAYHSNPVNIFIH             YLYFTPSFYTIPKSPCGF
Sbjct: 1   MGKTAFFDLERHFAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DRGLAFNFGFLFTLIYAASYVVFDKKAGSNAALPCLICWVGASILAFRLGYSQTWKIVLT 120
           D GL  NFGFLFTLIYAASYV+FDK+AGS AAL C +CWVGAS ++ RLGYSQTWK+VL 
Sbjct: 61  DHGLVLNFGFLFTLIYAASYVIFDKRAGSMAALLCFVCWVGASFISNRLGYSQTWKVVLA 120

Query: 121 AQLFCWTNQLIDHGVFEVRQKRAPALLDNLAQPFLMAPFFVFFEVLQSLFKYEPYPGFSA 180
           AQLFCWTNQ I HGVFE   KRAPALLDNLAQ FLMAPFFVF EVLQ+LFKYEPYPGFSA
Sbjct: 121 AQLFCWTNQFIGHGVFE---KRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSA 180

Query: 181 SVQAKIKADIKEWKEQKEKLT 189
           SVQAKI+ADIKEWKE+KEKL+
Sbjct: 181 SVQAKIEADIKEWKEKKEKLS 198

[Close]

BLAST of CmoCh10G002170.1 vs. ExPASy TrEMBL
Match: A0A6J1IBP8 (uncharacterized endoplasmic reticulum membrane protein C16E8.02 OS=Cucurbita maxima OX=3661 GN=LOC111471498 PE=4 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 9.6e-85
Identity = 158/201 (78.61%), Postives = 169/201 (84.08%), Query Frame = 0


Query: 1   MGKTGLFDLERHFAFYGAYHSNPVNIFIH-------------YLYFTPSFYTIPKSPCGF 60
           MGKT  FDLERH+AFYGAYHSNPVNIFIH             YLYFTPSFYTIPKSPCGF
Sbjct: 1   MGKTAFFDLERHYAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DRGLAFNFGFLFTLIYAASYVVFDKKAGSNAALPCLICWVGASILAFRLGYSQTWKIVLT 120
           D GL  NFGFLFTL YAASYV+FDKKAGS AAL C +CWVGAS ++ RLGYSQTWK+VL 
Sbjct: 61  DHGLVLNFGFLFTLTYAASYVIFDKKAGSMAALLCFVCWVGASFISNRLGYSQTWKVVLA 120

Query: 121 AQLFCWTNQLIDHGVFEVRQKRAPALLDNLAQPFLMAPFFVFFEVLQSLFKYEPYPGFSA 180
           AQLFCWTNQ+I HGVFE   KRAPALLDNLAQ FLMAPFFVF EVLQ+LFKYEPYPGFS+
Sbjct: 121 AQLFCWTNQIIGHGVFE---KRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSS 180

Query: 181 SVQAKIKADIKEWKEQKEKLT 189
           SVQAKI+ADIKEWKE KEKL+
Sbjct: 181 SVQAKIEADIKEWKEMKEKLS 198

[Close]

BLAST of CmoCh10G002170.1 vs. ExPASy TrEMBL
Match: A0A0A0LP54 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G009840 PE=4 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 1.4e-83
Identity = 158/201 (78.61%), Postives = 168/201 (83.58%), Query Frame = 0


Query: 1   MGKTGLFDLERHFAFYGAYHSNPVNIFIH-------------YLYFTPSFYTIPKSPCGF 60
           MGKTGLFDLE+ FAFYGAYHSNP+NIFIH             YLYFTPSFYTIPKSPCGF
Sbjct: 1   MGKTGLFDLEKQFAFYGAYHSNPMNIFIHVLFVWPIFFTSLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DRGLAFNFGFLFTLIYAASYVVFDKKAGSNAALPCLICWVGASILAFRLGYSQTWKIVLT 120
           D GL  NFGFLFTL+YAA YVVFDK+AGS AAL C +CWVGAS +A +LGYSQTWK+VL 
Sbjct: 61  DHGLVLNFGFLFTLMYAAYYVVFDKRAGSMAALLCFVCWVGASFIANKLGYSQTWKVVLA 120

Query: 121 AQLFCWTNQLIDHGVFEVRQKRAPALLDNLAQPFLMAPFFVFFEVLQSLFKYEPYPGFSA 180
           AQLFCWTNQ I HGVFE   KRAPALLDNLAQ FLMAPFFV  EVLQSLFKYEPYPGFSA
Sbjct: 121 AQLFCWTNQFIGHGVFE---KRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSA 180

Query: 181 SVQAKIKADIKEWKEQKEKLT 189
           SVQAKIKADI+EWKE KEKL+
Sbjct: 181 SVQAKIKADIREWKETKEKLS 198

[Close]

BLAST of CmoCh10G002170.1 vs. ExPASy TrEMBL
Match: A0A5A7UQ13 (Putative endoplasmic reticulum membrane protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002550 PE=4 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 3.1e-83
Identity = 157/201 (78.11%), Postives = 168/201 (83.58%), Query Frame = 0


Query: 1   MGKTGLFDLERHFAFYGAYHSNPVNIFIH-------------YLYFTPSFYTIPKSPCGF 60
           MGKTGLFDLE+ FAFYGAYHSNP+NIFIH             YLYFTPSFYTIPKSPCGF
Sbjct: 1   MGKTGLFDLEKQFAFYGAYHSNPMNIFIHVLFVWPIFFTSLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DRGLAFNFGFLFTLIYAASYVVFDKKAGSNAALPCLICWVGASILAFRLGYSQTWKIVLT 120
           D GL  NFGF FTL+YAA YVVFDK+AGS AAL C +CWVGAS +A +LGYSQTWK+VL 
Sbjct: 61  DHGLVLNFGFFFTLMYAAYYVVFDKRAGSMAALLCFVCWVGASYIANKLGYSQTWKVVLA 120

Query: 121 AQLFCWTNQLIDHGVFEVRQKRAPALLDNLAQPFLMAPFFVFFEVLQSLFKYEPYPGFSA 180
           AQLFCWTNQ I HGVFE   KRAPALLDNLAQ FLMAPFFV  EVLQSLFKYEPYPGFSA
Sbjct: 121 AQLFCWTNQFIGHGVFE---KRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSA 180

Query: 181 SVQAKIKADIKEWKEQKEKLT 189
           SVQAKIKADI+EWKE+KEKL+
Sbjct: 181 SVQAKIKADIREWKEKKEKLS 198

[Close]

BLAST of CmoCh10G002170.1 vs. ExPASy TrEMBL
Match: A0A1S3BV50 (uncharacterized endoplasmic reticulum membrane protein YGL010W OS=Cucumis melo OX=3656 GN=LOC103494003 PE=4 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 3.1e-83
Identity = 157/201 (78.11%), Postives = 168/201 (83.58%), Query Frame = 0


Query: 1   MGKTGLFDLERHFAFYGAYHSNPVNIFIH-------------YLYFTPSFYTIPKSPCGF 60
           MGKTGLFDLE+ FAFYGAYHSNP+NIFIH             YLYFTPSFYTIPKSPCGF
Sbjct: 1   MGKTGLFDLEKQFAFYGAYHSNPMNIFIHVLFVWPIFFTSLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DRGLAFNFGFLFTLIYAASYVVFDKKAGSNAALPCLICWVGASILAFRLGYSQTWKIVLT 120
           D GL  NFGF FTL+YAA YVVFDK+AGS AAL C +CWVGAS +A +LGYSQTWK+VL 
Sbjct: 61  DHGLVLNFGFFFTLMYAAYYVVFDKRAGSMAALLCFVCWVGASYIANKLGYSQTWKVVLA 120

Query: 121 AQLFCWTNQLIDHGVFEVRQKRAPALLDNLAQPFLMAPFFVFFEVLQSLFKYEPYPGFSA 180
           AQLFCWTNQ I HGVFE   KRAPALLDNLAQ FLMAPFFV  EVLQSLFKYEPYPGFSA
Sbjct: 121 AQLFCWTNQFIGHGVFE---KRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSA 180

Query: 181 SVQAKIKADIKEWKEQKEKLT 189
           SVQAKIKADI+EWKE+KEKL+
Sbjct: 181 SVQAKIKADIREWKEKKEKLS 198

[Close]

BLAST of CmoCh10G002170.1 vs. NCBI nr
Match: XP_038880038.1 (2-hydroxy-palmitic acid dioxygenase MPO1 [Benincasa hispida])

HSP 1 Score: 327.4 bits (838), Expect = 8.1e-86
Identity = 163/201 (81.09%), Postives = 171/201 (85.07%), Query Frame = 0


Query: 1   MGKTGLFDLERHFAFYGAYHSNPVNIFIH-------------YLYFTPSFYTIPKSPCGF 60
           MGK+GLFDLERHFAFYGAYHSNPVNIFIH             YLYFTPSFYTIPKSPCGF
Sbjct: 1   MGKSGLFDLERHFAFYGAYHSNPVNIFIHILFVWPIFFTSLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DRGLAFNFGFLFTLIYAASYVVFDKKAGSNAALPCLICWVGASILAFRLGYSQTWKIVLT 120
           D GL  NFGFLFTLIYAASYVVFDKKAGS AAL C +CWVGAS +A RLGYSQTWK+VL 
Sbjct: 61  DHGLVLNFGFLFTLIYAASYVVFDKKAGSMAALLCFVCWVGASFIANRLGYSQTWKVVLA 120

Query: 121 AQLFCWTNQLIDHGVFEVRQKRAPALLDNLAQPFLMAPFFVFFEVLQSLFKYEPYPGFSA 180
           AQLFCWTNQ I HGVFE   KRAPALLDNLAQ FLMAPFFV  EVLQSLFKYEPYPGFSA
Sbjct: 121 AQLFCWTNQFIGHGVFE---KRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSA 180

Query: 181 SVQAKIKADIKEWKEQKEKLT 189
           SVQAKIKADI+EWKE+K+KL+
Sbjct: 181 SVQAKIKADIEEWKEKKKKLS 198

[Close]

BLAST of CmoCh10G002170.1 vs. NCBI nr
Match: XP_022933403.1 (uncharacterized endoplasmic reticulum membrane protein C16E8.02 [Cucurbita moschata] >KAG7021523.1 SPAC16E8.02 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 325.5 bits (833), Expect = 3.1e-85
Identity = 160/201 (79.60%), Postives = 170/201 (84.58%), Query Frame = 0


Query: 1   MGKTGLFDLERHFAFYGAYHSNPVNIFIH-------------YLYFTPSFYTIPKSPCGF 60
           MGKT  FDLERHFAFYGAYHSNPVNIFIH             YLYFTPSFYTIPKSPCGF
Sbjct: 1   MGKTAFFDLERHFAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DRGLAFNFGFLFTLIYAASYVVFDKKAGSNAALPCLICWVGASILAFRLGYSQTWKIVLT 120
           D GL  NFGFLFTLIYAASYV+FDK+AGS AAL C +CWVGAS ++ RLGYSQTWK+VL 
Sbjct: 61  DHGLVLNFGFLFTLIYAASYVIFDKRAGSMAALLCFVCWVGASFISNRLGYSQTWKVVLA 120

Query: 121 AQLFCWTNQLIDHGVFEVRQKRAPALLDNLAQPFLMAPFFVFFEVLQSLFKYEPYPGFSA 180
           AQLFCWTNQ I HGVFE   KRAPALLDNLAQ FLMAPFFVF EVLQ+LFKYEPYPGFSA
Sbjct: 121 AQLFCWTNQFIGHGVFE---KRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSA 180

Query: 181 SVQAKIKADIKEWKEQKEKLT 189
           SVQAKI+ADIKEWKE+KEKL+
Sbjct: 181 SVQAKIEADIKEWKEKKEKLS 198

[Close]

BLAST of CmoCh10G002170.1 vs. NCBI nr
Match: XP_022972978.1 (uncharacterized endoplasmic reticulum membrane protein C16E8.02 [Cucurbita maxima])

HSP 1 Score: 322.8 bits (826), Expect = 2.0e-84
Identity = 158/201 (78.61%), Postives = 169/201 (84.08%), Query Frame = 0


Query: 1   MGKTGLFDLERHFAFYGAYHSNPVNIFIH-------------YLYFTPSFYTIPKSPCGF 60
           MGKT  FDLERH+AFYGAYHSNPVNIFIH             YLYFTPSFYTIPKSPCGF
Sbjct: 1   MGKTAFFDLERHYAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DRGLAFNFGFLFTLIYAASYVVFDKKAGSNAALPCLICWVGASILAFRLGYSQTWKIVLT 120
           D GL  NFGFLFTL YAASYV+FDKKAGS AAL C +CWVGAS ++ RLGYSQTWK+VL 
Sbjct: 61  DHGLVLNFGFLFTLTYAASYVIFDKKAGSMAALLCFVCWVGASFISNRLGYSQTWKVVLA 120

Query: 121 AQLFCWTNQLIDHGVFEVRQKRAPALLDNLAQPFLMAPFFVFFEVLQSLFKYEPYPGFSA 180
           AQLFCWTNQ+I HGVFE   KRAPALLDNLAQ FLMAPFFVF EVLQ+LFKYEPYPGFS+
Sbjct: 121 AQLFCWTNQIIGHGVFE---KRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSS 180

Query: 181 SVQAKIKADIKEWKEQKEKLT 189
           SVQAKI+ADIKEWKE KEKL+
Sbjct: 181 SVQAKIEADIKEWKEMKEKLS 198

[Close]

BLAST of CmoCh10G002170.1 vs. NCBI nr
Match: XP_023531593.1 (uncharacterized endoplasmic reticulum membrane protein C16E8.02 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 320.9 bits (821), Expect = 7.6e-84
Identity = 157/198 (79.29%), Postives = 167/198 (84.34%), Query Frame = 0


Query: 1   MGKTGLFDLERHFAFYGAYHSNPVNIFIH-------------YLYFTPSFYTIPKSPCGF 60
           MGKT  FDLERHFAFYGAYHSNP+NIFIH             YLYFTPSFYTIPKSPCGF
Sbjct: 1   MGKTAFFDLERHFAFYGAYHSNPINIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DRGLAFNFGFLFTLIYAASYVVFDKKAGSNAALPCLICWVGASILAFRLGYSQTWKIVLT 120
           D GL  NFGFLFTLIYAASYV+FDKKAGS AAL C +CWVGAS ++ RLGYSQTWK+VL 
Sbjct: 61  DHGLVLNFGFLFTLIYAASYVIFDKKAGSMAALLCFVCWVGASFISNRLGYSQTWKVVLA 120

Query: 121 AQLFCWTNQLIDHGVFEVRQKRAPALLDNLAQPFLMAPFFVFFEVLQSLFKYEPYPGFSA 180
           AQLFCWTNQ I HGVFE   KRAPALLDNLAQ FLMAPFFVF EVLQ+LFKYEPYPGFSA
Sbjct: 121 AQLFCWTNQFIGHGVFE---KRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSA 180

Query: 181 SVQAKIKADIKEWKEQKE 186
           SVQAKI+ADIKEWKE+K+
Sbjct: 181 SVQAKIEADIKEWKEKKK 195

[Close]

BLAST of CmoCh10G002170.1 vs. NCBI nr
Match: XP_004138174.1 (uncharacterized endoplasmic reticulum membrane protein YGL010W [Cucumis sativus] >KGN63680.1 hypothetical protein Csa_014278 [Cucumis sativus])

HSP 1 Score: 318.9 bits (816), Expect = 2.9e-83
Identity = 158/201 (78.61%), Postives = 168/201 (83.58%), Query Frame = 0


Query: 1   MGKTGLFDLERHFAFYGAYHSNPVNIFIH-------------YLYFTPSFYTIPKSPCGF 60
           MGKTGLFDLE+ FAFYGAYHSNP+NIFIH             YLYFTPSFYTIPKSPCGF
Sbjct: 1   MGKTGLFDLEKQFAFYGAYHSNPMNIFIHVLFVWPIFFTSLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DRGLAFNFGFLFTLIYAASYVVFDKKAGSNAALPCLICWVGASILAFRLGYSQTWKIVLT 120
           D GL  NFGFLFTL+YAA YVVFDK+AGS AAL C +CWVGAS +A +LGYSQTWK+VL 
Sbjct: 61  DHGLVLNFGFLFTLMYAAYYVVFDKRAGSMAALLCFVCWVGASFIANKLGYSQTWKVVLA 120

Query: 121 AQLFCWTNQLIDHGVFEVRQKRAPALLDNLAQPFLMAPFFVFFEVLQSLFKYEPYPGFSA 180
           AQLFCWTNQ I HGVFE   KRAPALLDNLAQ FLMAPFFV  EVLQSLFKYEPYPGFSA
Sbjct: 121 AQLFCWTNQFIGHGVFE---KRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSA 180

Query: 181 SVQAKIKADIKEWKEQKEKLT 189
           SVQAKIKADI+EWKE KEKL+
Sbjct: 181 SVQAKIKADIREWKETKEKLS 198

[Close]

BLAST of CmoCh10G002170.1 vs. TAIR 10
Match: AT1G18720.1 (Protein of unknown function (DUF962) )

HSP 1 Score: 216.5 bits (550), Expect = 1.9e-56
Identity = 112/199 (56.28%), Postives = 135/199 (67.84%), Query Frame = 0


Query: 5   GLFDLERHFAFYGAYHSNPVNIFIHYLYFTPSFYTI-----------PKSPCGFDRGLA- 64
           GLFDLE+HFAFYGAYHSNP+NI IH ++  P F+++             S  GF + L  
Sbjct: 6   GLFDLEKHFAFYGAYHSNPINILIHIIFVWPIFFSVLLLLHSSTPIFDPSQLGFSQSLTL 65

Query: 65  -----FNFGFLFTLIYAASYVVFDKKAGSNAALPCLICWVGASILAFRLGYSQTWKIVLT 124
                FN GF+F LIYA  Y+  DKK+G  AAL C  CWVG+S LA RLG S   K+ L 
Sbjct: 66  DGVLRFNVGFIFALIYALFYIGLDKKSGFVAALMCFSCWVGSSFLAVRLGSSLALKVGLA 125

Query: 125 AQLFCWTNQLIDHGVFEVRQKRAPALLDNLAQPFLMAPFFVFFEVLQSLFKYEPYPGFSA 184
           +QL CWT Q + HGVFE   KRAPALLDNL Q FLMAPFFV  EVLQS+F YEPYPGF A
Sbjct: 126 SQLLCWTGQFVGHGVFE---KRAPALLDNLLQAFLMAPFFVLLEVLQSVFGYEPYPGFQA 185

Query: 185 SVQAKIKADIKEWKEQKEK 187
            V AK+++DIKE++ +K+K
Sbjct: 186 RVNAKVESDIKEYRAKKQK 201

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BLAST of CmoCh10G002170.1 vs. TAIR 10
Match: AT1G74440.1 (Protein of unknown function (DUF962) )

HSP 1 Score: 205.7 bits (522), Expect = 3.3e-53
Identity = 105/201 (52.24%), Postives = 133/201 (66.17%), Query Frame = 0


Query: 3   KTGLFDLERHFAFYGAYHSNPVNIFIHYLYFTPSFYT-----------IPKSPCGFDRGL 62
           + GL DLE+HFAFYGAYHSNP+NI IH L+  P+ +            +  S  GF + L
Sbjct: 4   RIGLLDLEKHFAFYGAYHSNPINIIIHTLFVWPNVFATLLFLYSTPPILDHSQLGFLKSL 63

Query: 63  AF------NFGFLFTLIYAASYVVFDKKAGSNAALPCLICWVGASILAFRLGYSQTWKIV 122
            F      + GF  T+ YA  Y+  DKK+G  AAL C  CW+G+S LA RLG+S T K+ 
Sbjct: 64  TFDGVLRLDIGFTLTVTYAVFYICLDKKSGVLAALLCFSCWIGSSFLAARLGHSLTLKVG 123

Query: 123 LTAQLFCWTNQLIDHGVFEVRQKRAPALLDNLAQPFLMAPFFVFFEVLQSLFKYEPYPGF 182
           + +QL CWT Q + HG+FE   KRAPALLDNL Q FLM PFFV  EVLQS+F YEPYPGF
Sbjct: 124 VASQLLCWTGQFLGHGLFE---KRAPALLDNLLQAFLMGPFFVLLEVLQSVFGYEPYPGF 183

Query: 183 SASVQAKIKADIKEWKEQKEK 187
            A V +KI++ IKEW+E+K++
Sbjct: 184 KARVDSKIESGIKEWREKKKQ 201

The following BLAST results are available for this feature:

BLAST of CmoCh10G002170.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O13737	1.0e-14	35.80	2-hydroxy-palmitic acid dioxygenase mpo1 OS=Schizosaccharomyces pombe (strain 97...	[more]
P25338	3.0e-11	29.83	2-hydroxy-palmitic acid dioxygenase MPO1 OS=Saccharomyces cerevisiae (strain ATC...	[more]

BLAST of CmoCh10G002170.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F4N0	1.5e-85	79.60	uncharacterized endoplasmic reticulum membrane protein C16E8.02 OS=Cucurbita mos...	[more]
A0A6J1IBP8	9.6e-85	78.61	uncharacterized endoplasmic reticulum membrane protein C16E8.02 OS=Cucurbita max...	[more]
A0A0A0LP54	1.4e-83	78.61	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G009840 PE=4 SV=1	[more]
A0A5A7UQ13	3.1e-83	78.11	Putative endoplasmic reticulum membrane protein OS=Cucumis melo var. makuwa OX=1...	[more]
A0A1S3BV50	3.1e-83	78.11	uncharacterized endoplasmic reticulum membrane protein YGL010W OS=Cucumis melo O...	[more]

BLAST of CmoCh10G002170.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038880038.1	8.1e-86	81.09	2-hydroxy-palmitic acid dioxygenase MPO1 [Benincasa hispida]	[more]
XP_022933403.1	3.1e-85	79.60	uncharacterized endoplasmic reticulum membrane protein C16E8.02 [Cucurbita mosch...	[more]
XP_022972978.1	2.0e-84	78.61	uncharacterized endoplasmic reticulum membrane protein C16E8.02 [Cucurbita maxim...	[more]
XP_023531593.1	7.6e-84	79.29	uncharacterized endoplasmic reticulum membrane protein C16E8.02 [Cucurbita pepo ...	[more]
XP_004138174.1	2.9e-83	78.61	uncharacterized endoplasmic reticulum membrane protein YGL010W [Cucumis sativus]...	[more]

BLAST of CmoCh10G002170.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G18720.1	1.9e-56	56.28	Protein of unknown function (DUF962)	[more]
AT1G74440.1	3.3e-53	52.24	Protein of unknown function (DUF962)	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009305	2-hydroxy-palmitic acid dioxygenase Mpo1-like	PFAM	PF06127	DUF962	coord: 8..155 e-value: 2.5E-17 score: 62.8
IPR009305	2-hydroxy-palmitic acid dioxygenase Mpo1-like	PANTHER	PTHR28026	DUF962 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_8G05310)	coord: 1..187
None	No IPR available	PANTHER	PTHR28026:SF8	YGL010W-LIKE PROTEIN	coord: 1..187

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmoCh10G002170	CmoCh10G002170	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh10G002170.1:exon:6466	CmoCh10G002170.1:exon:6466	exon
CmoCh10G002170.1:exon:6467	CmoCh10G002170.1:exon:6467	exon
CmoCh10G002170.1:exon:6468	CmoCh10G002170.1:exon:6468	exon
CmoCh10G002170.1:exon:6469	CmoCh10G002170.1:exon:6469	exon
CmoCh10G002170.1:exon:6470	CmoCh10G002170.1:exon:6470	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh10G002170.1:cds	CmoCh10G002170.1:cds	CDS
CmoCh10G002170.1:cds	CmoCh10G002170.1:cds_2	CDS
CmoCh10G002170.1:cds	CmoCh10G002170.1:cds_3	CDS
CmoCh10G002170.1:cds	CmoCh10G002170.1:cds_4	CDS
CmoCh10G002170.1:cds	CmoCh10G002170.1:cds_5	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh10G002170.1:three_prime_utr	CmoCh10G002170.1:three_prime_utr	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmoCh10G002170.1	CmoCh10G002170.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046521	sphingoid catabolic process
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane