CmoCh05G007420.1 (mRNA) Cucurbita moschata (Rifu) v1

BLAST of CmoCh05G007420.1 vs. ExPASy Swiss-Prot
Match: Q9SZW4 (Cadmium/zinc-transporting ATPase HMA2 OS=Arabidopsis thaliana OX=3702 GN=HMA2 PE=2 SV=1)

HSP 1 Score: 856.7 bits (2212), Expect = 2.1e-247
Identity = 452/723 (62.52%), Postives = 554/723 (76.63%), Query Frame = 0

Query: 18  KFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTLI---------------- 77
           K  +S+FDVLGICC+SEVPLIENIL  ++G+K+ SVIVP+RT+I                
Sbjct: 5   KMTKSYFDVLGICCTSEVPLIENILNSMDGVKEFSVIVPSRTVIVVHDTLILSQFQIVKA 64

Query: 78  -------ANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVYHPLRWLAVAAVAAGIFP 137
                  ANV++ G+   K KWPSPFA+ SG+LL  SF KY+Y P RWLAVAAV AGI+P
Sbjct: 65  LNQAQLEANVRVTGETNFKNKWPSPFAVVSGILLLLSFFKYLYSPFRWLAVAAVVAGIYP 124

Query: 138 ILLKAISAVRHLRIDVNILAIIAVVGAMSMNDYMEAGSIVFLFSIAEWLESRASHKANAA 197
           IL KA++++   RID+NIL ++ V   + M DY EA  +VFLF+IAEWL+SRAS+KA+A 
Sbjct: 125 ILAKAVASLARFRIDINILVVVTVGATIGMQDYTEAAVVVFLFTIAEWLQSRASYKASAV 184

Query: 198 MWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPIDGIVVDGKCDVDEKSLTG 257
           M SLMSLAPQKA IAETGE VEV E+   +V+AVK GE IPIDG+VVDG C+VDEK+LTG
Sbjct: 185 MQSLMSLAPQKAVIAETGEEVEVDELKTNTVIAVKAGETIPIDGVVVDGNCEVDEKTLTG 244

Query: 258 ETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEFVEEAQNNKSKTQRFIDE 317
           E FPVPKLKDS VWAGTINLNGYI+V TT +AEDCVVAKMA+ VEEAQN+K++TQRFID+
Sbjct: 245 EAFPVPKLKDSTVWAGTINLNGYITVNTTALAEDCVVAKMAKLVEEAQNSKTETQRFIDK 304

Query: 318 CAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSACPCALILSTPVAAFCALT 377
           C+KYYTPA+++IS C  AIP A +VHNL HW HLALVVLVSACPC LILSTPVA FCALT
Sbjct: 305 CSKYYTPAIILISICFVAIPFALKVHNLKHWVHLALVVLVSACPCGLILSTPVATFCALT 364

Query: 378 KAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVIFKLYEMISASTPYFIGNCTKN 437
           KAA +G+LIKG+D+LE LAKIK+VAFDKTGTITRGEF++     +            +++
Sbjct: 365 KAATSGLLIKGADYLETLAKIKIVAFDKTGTITRGEFIVMDFQSL------------SED 424

Query: 438 FSPNSVYVEIRVSSIESKSSHPMAAALVNHAKLLSTDIKPEKVEEFENFPGEGVRGKIDG 497
            S  S+     VSS ESKSSHPMAAA+V++A+ +S + KPE VE+++NFPGEG+ GKIDG
Sbjct: 425 ISLQSLL--YWVSSTESKSSHPMAAAVVDYARSVSVEPKPEAVEDYQNFPGEGIYGKIDG 484

Query: 498 KDIYIGKE----------------EMKQGQTLGYVFCEEMAVGSFGLSDSCRSGAKEGME 557
           K++YIG +                + K G+T+GYV+  E   G F LSD+CRSG  + M+
Sbjct: 485 KEVYIGNKRIASRAGCLSVPDIDVDTKGGKTIGYVYVGETLAGVFNLSDACRSGVAQAMK 544

Query: 558 ELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRDYGATAMV 617
           ELKSLGIK AMLTGD  AAAMHAQ+QL NA+D++ +ELLP++K+ +IK+ KR+ G TAMV
Sbjct: 545 ELKSLGIKIAMLTGDNHAAAMHAQEQLGNAMDIVRAELLPEDKSEIIKQLKREEGPTAMV 604

Query: 618 GDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKVVQN 677
           GDGLND PALATADIGISMG+SGS LAT+TGN+ILMSNDI +IPQAIKLA+++  KVV+N
Sbjct: 605 GDGLNDAPALATADIGISMGVSGSALATETGNIILMSNDIRRIPQAIKLAKRAKRKVVEN 664

Query: 678 VILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCKSSK 702
           V++SIT K AIL LA AGHPL+WAAVLADVGTCLLVILNSMLLL    HK GNKC + S 
Sbjct: 665 VVISITMKGAILALAFAGHPLIWAAVLADVGTCLLVILNSMLLL-SDKHKTGNKCYRESS 712

BLAST of CmoCh05G007420.1 vs. ExPASy Swiss-Prot
Match: O64474 (Putative cadmium/zinc-transporting ATPase HMA4 OS=Arabidopsis thaliana OX=3702 GN=HMA4 PE=1 SV=2)

HSP 1 Score: 828.2 bits (2138), Expect = 8.2e-239
Identity = 474/823 (57.59%), Postives = 596/823 (72.42%), Query Frame = 0

Query: 8   EKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTLI------ 67
           E+ KKV+   K ++S+FDVLGICC+SEVP+IENILK L+G+K+ SVIVP+RT+I      
Sbjct: 8   EEKKKVK---KLQKSYFDVLGICCTSEVPIIENILKSLDGVKEYSVIVPSRTVIVVHDSL 67

Query: 68  -----------------ANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVYHPLRWLA 127
                            ANV++ G+   K KWPSPFA+ SGLLL  SFLK+VY PLRWLA
Sbjct: 68  LISPFQIAKALNEARLEANVRVNGETSFKNKWPSPFAVVSGLLLLLSFLKFVYSPLRWLA 127

Query: 128 VAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGAMSMNDYMEAGSIVFLFSIAEWLE 187
           VAAVAAGI+PIL KA ++++  RID+NIL II V+  ++M D+MEA ++VFLF+I++WLE
Sbjct: 128 VAAVAAGIYPILAKAFASIKRPRIDINILVIITVIATLAMQDFMEAAAVVFLFTISDWLE 187

Query: 188 SRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPIDGIVVDGK 247
           +RAS+KA + M SLMSLAPQKA IAETGE VEV EV + +V+AVK GE IPIDGIVVDG 
Sbjct: 188 TRASYKATSVMQSLMSLAPQKAIIAETGEEVEVDEVKVDTVVAVKAGETIPIDGIVVDGN 247

Query: 248 CDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEFVEEAQNN 307
           C+VDEK+LTGE FPVPK +DS VWAGTINLNGYI V+TT +A DCVVAKMA+ VEEAQ++
Sbjct: 248 CEVDEKTLTGEAFPVPKQRDSTVWAGTINLNGYICVKTTSLAGDCVVAKMAKLVEEAQSS 307

Query: 308 KSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSACPCALILS 367
           K+K+QR ID+C++YYTPA++++SACVA +P   +VHNL HWFHLALVVLVS CPC LILS
Sbjct: 308 KTKSQRLIDKCSQYYTPAIILVSACVAIVPVIMKVHNLKHWFHLALVVLVSGCPCGLILS 367

Query: 368 TPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVIFKLYEMISAST 427
           TPVA FCALTKAA +G+LIK +D+L+ L+KIK+VAFDKTGTITRGEF++     +     
Sbjct: 368 TPVATFCALTKAATSGLLIKSADYLDTLSKIKIVAFDKTGTITRGEFIVIDFKSL----- 427

Query: 428 PYFIGNCTKNFSPNSVYVEIRVSSIESKSSHPMAAALVNHAKLLSTDIKPEKVEEFENFP 487
                  +++ +  S+     VSS+ESKSSHPMAA +V++AK +S + +PE+VE+++NFP
Sbjct: 428 -------SRDINLRSLL--YWVSSVESKSSHPMAATIVDYAKSVSVEPRPEEVEDYQNFP 487

Query: 488 GEGVRGKIDGKDIYIGKEEM----------------KQGQTLGYVFCEEMAVGSFGLSDS 547
           GEG+ GKIDG DI+IG +++                K G+T+GYV+  E   G F LSD+
Sbjct: 488 GEGIYGKIDGNDIFIGNKKIASRAGCSTVPEIEVDTKGGKTVGYVYVGERLAGFFNLSDA 547

Query: 548 CRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEF 607
           CRSG  + M ELKSLGIKTAMLTGD  AAAMHAQ+QL N +DV+H +LLP++K+ +I+EF
Sbjct: 548 CRSGVSQAMAELKSLGIKTAMLTGDNQAAAMHAQEQLGNVLDVVHGDLLPEDKSRIIQEF 607

Query: 608 KRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLA 667
           K++ G TAMVGDG+ND PALATADIGISMGISGS LATQTGN+ILMSNDI +IPQA+KLA
Sbjct: 608 KKE-GPTAMVGDGVNDAPALATADIGISMGISGSALATQTGNIILMSNDIRRIPQAVKLA 667

Query: 668 RKSHAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHK 727
           R++  KVV+NV LSI  K  IL LA AGHPL+WAAVL DVGTCLLVI NSMLLLR    K
Sbjct: 668 RRARRKVVENVCLSIILKAGILALAFAGHPLIWAAVLVDVGTCLLVIFNSMLLLR-EKKK 727

Query: 728 HGNKCC---KSSKLCSTK-HGRCD-------GSNTRPSHHHHHDHHHHD--HRCHVV--- 775
            GNK C    +SKL   K  G  D       G  T+  +         D  ++ +VV   
Sbjct: 728 IGNKKCYRASTSKLNGRKLEGDDDYVVDLEAGLLTKSGNGQCKSSCCGDKKNQENVVMMK 787

BLAST of CmoCh05G007420.1 vs. ExPASy Swiss-Prot
Match: P0CW78 (Cadmium/zinc-transporting ATPase HMA3 OS=Arabidopsis thaliana OX=3702 GN=HMA3 PE=1 SV=1)

HSP 1 Score: 761.9 bits (1966), Expect = 7.2e-219
Identity = 412/731 (56.36%), Postives = 529/731 (72.37%), Query Frame = 0

Query: 8   EKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTLI------ 67
           E++KK+      + S+FDV+GICCSSEV ++ N+L+ ++G+K+ SVIVP+RT+I      
Sbjct: 5   EESKKM----NLQTSYFDVVGICCSSEVSIVGNVLRQVDGVKEFSVIVPSRTVIVVHDTF 64

Query: 68  -----------------ANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVYHPLRWLA 127
                            A+V+  G+   K +WPSPFAI SG+LL  SF KY Y PL WLA
Sbjct: 65  LISPLQIVKALNQARLEASVRPYGETSLKSQWPSPFAIVSGVLLVLSFFKYFYSPLEWLA 124

Query: 128 VAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGAMSMNDYMEAGSIVFLFSIAEWLE 187
           + AV AG+FPIL KA+++V   R+D+N L +IAV+  + M D+ EA +IVFLFS+A+WLE
Sbjct: 125 IVAVVAGVFPILAKAVASVTRFRLDINALTLIAVIATLCMQDFTEAATIVFLFSVADWLE 184

Query: 188 SRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPIDGIVVDGK 247
           S A+HKA+  M SLMSLAP+KA IA+TG  V+V EV + +V++VK GE IPIDG+VVDG 
Sbjct: 185 SSAAHKASIVMSSLMSLAPRKAVIADTGLEVDVDEVGINTVVSVKAGESIPIDGVVVDGS 244

Query: 248 CDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEFVEEAQNN 307
           CDVDEK+LTGE+FPV K ++S V A TINLNGYI V+TT +A DCVVAKM + VEEAQ +
Sbjct: 245 CDVDEKTLTGESFPVSKQRESTVMAATINLNGYIKVKTTALARDCVVAKMTKLVEEAQKS 304

Query: 308 KSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSACPCALILS 367
           ++KTQRFID+C++YYTPAVVV +AC A IP   +V +LSHWFHLALVVLVS CPC LILS
Sbjct: 305 QTKTQRFIDKCSRYYTPAVVVSAACFAVIPVLLKVQDLSHWFHLALVVLVSGCPCGLILS 364

Query: 368 TPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVIFKLYEMISAST 427
           TPVA FCALTKAA +G LIK  D LE LAKIK+VAFDKTGTIT+ EF++     +  +  
Sbjct: 365 TPVATFCALTKAATSGFLIKTGDCLETLAKIKIVAFDKTGTITKAEFMVSDFRSLSPSIN 424

Query: 428 PYFIGNCTKNFSPNSVYVEIRVSSIESKSSHPMAAALVNHAKLLSTDIKPEKVEEFENFP 487
            + + N               VSSIE KSSHPMAAAL+++A  +S + KP+ VE F+NFP
Sbjct: 425 LHKLLNW--------------VSSIECKSSHPMAAALIDYAISVSVEPKPDIVENFQNFP 484

Query: 488 GEGVRGKIDGKDIYIGKEE------------------MKQGQTLGYVFCEEMAVGSFGLS 547
           GEGV G+IDG+DIYIG +                   MK+G+T+GY++      GSF L 
Sbjct: 485 GEGVYGRIDGQDIYIGNKRIAQRAGCLTDNVPDIEATMKRGKTIGYIYMGAKLTGSFNLL 544

Query: 548 DSCRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIK 607
           D CR G  + ++ELKSLGI+TAMLTGD   AAM  Q+QLENA+D++HSELLP++KA +I 
Sbjct: 545 DGCRYGVAQALKELKSLGIQTAMLTGDNQDAAMSTQEQLENALDIVHSELLPQDKARIID 604

Query: 608 EFKRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIK 667
           +FK   G T MVGDGLND PALA ADIGISMGISGS LAT+TG++ILMSNDI KIP+ ++
Sbjct: 605 DFKIQ-GPTMMVGDGLNDAPALAKADIGISMGISGSALATETGDIILMSNDIRKIPKGMR 664

Query: 668 LARKSHAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSD 698
           LA++SH KV++NV+LS++ K AI+ L   G+PLVWAAVLAD GTCLLVILNSM+LLR  +
Sbjct: 665 LAKRSHKKVIENVVLSVSIKGAIMVLGFVGYPLVWAAVLADAGTCLLVILNSMILLR-DE 715

BLAST of CmoCh05G007420.1 vs. ExPASy Swiss-Prot
Match: A3BF39 (Cadmium/zinc-transporting ATPase HMA2 OS=Oryza sativa subsp. japonica OX=39947 GN=HMA2 PE=1 SV=1)

HSP 1 Score: 748.4 bits (1931), Expect = 8.2e-215
Identity = 432/881 (49.04%), Postives = 570/881 (64.70%), Query Frame = 0

Query: 20  ERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTLI------------------ 79
           ++S+FDVLGICC SEVPL+E +L+PLEG+++++VIVP+RT+I                  
Sbjct: 9   QKSYFDVLGICCPSEVPLVEKLLQPLEGVQKVTVIVPSRTVIVVHDVDAISQSQIVKALN 68

Query: 80  -----ANVQLKGKGISK--KKWPSPFAIASGLLLTASFLKYVYHPLRWLAVAAVAAGIFP 139
                A+V+  G G  K   KWPSP+ +  GLLL  S  ++ +HPL+W A+ A AAG+ P
Sbjct: 69  QARLEASVRAYGNGSEKITNKWPSPYVLLCGLLLVVSLFEHFWHPLKWFALVAAAAGLPP 128

Query: 140 ILLKAISAVRHLRIDVNILAIIAVVGAMSMNDYMEAGSIVFLFSIAEWLESRASHKANAA 199
           I+L++I+A+R L +DVNIL +IAV GA+++ DY EAG IVFLF+ AEWLE+RASHKA A 
Sbjct: 129 IVLRSIAAIRRLTLDVNILMLIAVAGAIALKDYSEAGFIVFLFTTAEWLETRASHKATAG 188

Query: 200 MWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPIDGIVVDGKCDVDEKSLTG 259
           M +LMS+APQKA +AETGEVV  ++V + +V+AVK GEVIPIDG+VVDG+ +VDE +LTG
Sbjct: 189 MSALMSMAPQKAILAETGEVVAARDVKVNTVIAVKAGEVIPIDGVVVDGRSEVDESTLTG 248

Query: 260 ETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEFVEEAQNNKSKTQRFIDE 319
           E+FPV K  DS VWAGT+N++GYI+V+TT +A++  VAKMA  VEEAQN++S TQR ID 
Sbjct: 249 ESFPVSKQPDSQVWAGTLNIDGYIAVRTTAMADNSAVAKMARLVEEAQNSRSSTQRLIDT 308

Query: 320 CAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSACPCALILSTPVAAFCALT 379
           CAKYYTPAVVV++  VAAIPA  + HNL HWF LALV+LVSACPCAL+LSTP+A FCAL 
Sbjct: 309 CAKYYTPAVVVMAGSVAAIPAIAKAHNLKHWFQLALVLLVSACPCALVLSTPIATFCALL 368

Query: 380 KAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVIFKLYEMISASTPYFIGNCTKN 439
           +AA  G+LIKG D LE LA IKV AFDKTGTITRGEF +                   + 
Sbjct: 369 RAARTGLLIKGGDVLESLASIKVAAFDKTGTITRGEFSV-------------------EE 428

Query: 440 FSPNSVYVEIR-----VSSIESKSSHPMAAALVNHAKLLSTDIKPEKVEEFENFPGEGVR 499
           F P    V ++     VSS+ES+SSHPMA+ LV++A+  S + K E V EF+ +PGEG+ 
Sbjct: 429 FQPVGERVSLQQLLYWVSSVESRSSHPMASVLVDYAQSKSVEPKSENVSEFQIYPGEGIY 488

Query: 500 GKIDGKDIYIGKE------------EMK--QGQTLGYVFCEEMAVGSFGLSDSCRSGAKE 559
           G+IDG  IYIG +            +MK  +G T+GYV C    +G F LSD+CR+G+ E
Sbjct: 489 GEIDGAGIYIGNKRILSRASCETVPDMKDMKGVTIGYVACNNELIGVFTLSDACRTGSAE 548

Query: 560 GMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRDYGAT 619
            ++EL+SLGIK+ MLTGD SAAA +AQ QL N +  +H+ELLP++K  ++ E K   G T
Sbjct: 549 AIKELRSLGIKSVMLTGDSSAAATYAQNQLGNILAEVHAELLPEDKVRIVGELKEKDGPT 608

Query: 620 AMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKV 679
            MVGDG+ND PALA AD+G+SMG+SGS +A +T +V LMSNDI +IP+A++LAR++H  +
Sbjct: 609 LMVGDGMNDAPALAKADVGVSMGVSGSAVAMETSHVALMSNDIRRIPKAVRLARRTHRTI 668

Query: 680 VQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCK 739
           + N+I S+ TK+AI+GLA AGHPL+WAAVLADVGTCLLVI+ SMLLLR  D +   KC  
Sbjct: 669 IVNIIFSVITKLAIVGLAFAGHPLIWAAVLADVGTCLLVIMYSMLLLREKDSRKAKKCAA 728

Query: 740 S-----SKLCSTK-HGRCDGSNTRPSHH------------------------HHHDHHHH 799
           S      K CS+  HG     N   SHH                        HHH+H+HH
Sbjct: 729 SHHGSPKKCCSSSHHGSHAKKNHGVSHHCSDGPCKSMVSCKESSVAKNACHDHHHEHNHH 788

BLAST of CmoCh05G007420.1 vs. ExPASy Swiss-Prot
Match: Q8H384 (Cadmium/zinc-transporting ATPase HMA3 OS=Oryza sativa subsp. japonica OX=39947 GN=HMA3 PE=1 SV=1)

HSP 1 Score: 661.4 bits (1705), Expect = 1.3e-188
Identity = 359/705 (50.92%), Postives = 481/705 (68.23%), Query Frame = 0

Query: 18  KFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTLI---------------- 77
           K ++++ DVLG+CCS+EV L+E +L PL+G++ +SV+V +RT++                
Sbjct: 40  KRKKTYLDVLGVCCSAEVALVERLLAPLDGVRVVSVVVASRTVVVEHDPAAAPESAIVKA 99

Query: 78  -------ANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVYHPLRWLAVAAVAAGIFP 137
                  A+V+  G      +WPSP+ +ASG+LLTASF ++++ PL+ LAVAAV AG  P
Sbjct: 100 LNKAGLEASVRAYGSSGVVSRWPSPYIVASGVLLTASFFEWLFPPLQCLAVAAVVAGAPP 159

Query: 138 ILLKAISAVRHLRIDVNILAIIAVVGAMSMNDYMEAGSIVFLFSIAEWLESRASHKANAA 197
           ++ +  +A   L +D+N+L +IAV GA+ + DY EAG+IVFLF+ AEWLE+ A  KA+A 
Sbjct: 160 MVRRGFAAASRLSLDINVLMLIAVAGALCLGDYTEAGAIVFLFTTAEWLETLACTKASAG 219

Query: 198 MWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPIDGIVVDGKCDVDEKSLTG 257
           M SLM + P KA IA TGEVV V++V +  V+AV+ GE++P+DG+VVDG+ +VDE+SLTG
Sbjct: 220 MSSLMGMLPVKAVIATTGEVVSVRDVRVGDVVAVRAGEIVPVDGVVVDGQSEVDERSLTG 279

Query: 258 ETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEFVEEAQNNKSKTQRFIDE 317
           E+FPVPK   S VWAGT+N +GYI+V+TT +AE+  VAKM   VE AQN++SKTQR ID 
Sbjct: 280 ESFPVPKQPHSEVWAGTMNFDGYIAVRTTALAENSTVAKMERLVEAAQNSRSKTQRLIDS 339

Query: 318 CAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSACPCALILSTPVAAFCALT 377
           CAKYYTPAVVV++A VA IPA      L  W+ LALV+LVSACPCAL+LSTPVA+FCA+ 
Sbjct: 340 CAKYYTPAVVVVAAGVALIPALLGADGLEQWWKLALVMLVSACPCALVLSTPVASFCAML 399

Query: 378 KAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVIFKLYEMISASTPYFIGNCTKN 437
           +AA  G+ IKG D LE L +I+ VAFDKTGTITRGEF         S  + + +G+    
Sbjct: 400 RAARMGIFIKGGDVLESLGEIRAVAFDKTGTITRGEF---------SIDSFHLVGDHKVE 459

Query: 438 FSPNSVYVEIRVSSIESKSSHPMAAALVNHAKLLSTDIKPEKVEEFENFPGEGVRGKIDG 497
                 ++   ++SIESKSSHPMAAALV +A+  S    PE V +F  +PGEG+ G+I G
Sbjct: 460 MD----HLLYWIASIESKSSHPMAAALVEYAQSKSIQPNPENVGDFRIYPGEGIYGEIHG 519

Query: 498 KDIYIGKE----------------EMKQGQTLGYVFCEEMAVGSFGLSDSCRSGAKEGME 557
           K IYIG                  EM +G ++GYV C+    G F LSD CR+GA E + 
Sbjct: 520 KHIYIGNRRTLARASSPQSTQEMGEMIKGVSIGYVICDGELAGVFSLSDDCRTGAAEAIR 579

Query: 558 ELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRDYGATAMV 617
           EL SLGIK+ MLTGD SAAA HAQ QL   ++ +HSELLP++K  ++   K  +G T MV
Sbjct: 580 ELGSLGIKSVMLTGDSSAAATHAQGQLGGVMEELHSELLPEDKVRLVSGLKARFGPTMMV 639

Query: 618 GDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKVVQN 677
           GDG+ND  ALA AD+G+SMGISGS  A +T +  LMS+D+ ++P+A++L R +   +  N
Sbjct: 640 GDGMNDAAALAAADVGVSMGISGSAAAMETSHATLMSSDVLRVPEAVRLGRCARRTIAVN 699

Query: 678 VILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLR 684
           V  S+  K A+L LA A  P++WAAVLADVGTCLLV+LNSM LLR
Sbjct: 700 VAGSVAVKAAVLALAAAWRPVLWAAVLADVGTCLLVVLNSMTLLR 731

BLAST of CmoCh05G007420.1 vs. ExPASy TrEMBL
Match: A0A6J1EPB0 (cadmium/zinc-transporting ATPase HMA2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436148 PE=3 SV=1)

HSP 1 Score: 1456.4 bits (3769), Expect = 0.0e+00
Identity = 769/842 (91.33%), Postives = 776/842 (92.16%), Query Frame = 0

Query: 1   MVGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL 60
           MVGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL
Sbjct: 1   MVGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL 60

Query: 61  I-----------------------ANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY 120
           I                       ANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY
Sbjct: 61  IVVHDSLLVSQLQIVKALNEARLEANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY 120

Query: 121 HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGAMSMNDYMEAGSIVFLF 180
           HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGAMSMNDYMEAGSIVFLF
Sbjct: 121 HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGAMSMNDYMEAGSIVFLF 180

Query: 181 SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPID 240
           SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPID
Sbjct: 181 SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPID 240

Query: 241 GIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF 300
           GIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF
Sbjct: 241 GIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF 300

Query: 301 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 360
           VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC
Sbjct: 301 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 360

Query: 361 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVIFKLY 420
           PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFV+    
Sbjct: 361 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTDFQ 420

Query: 421 EMISASTPYFIGNCTKNFSPNSVYVEIRVSSIESKSSHPMAAALVNHAKLLSTDIKPEKV 480
            +    + + + +               VSSIESKSSHPMAAALVNHAKLLSTDIKPEKV
Sbjct: 421 ALRDDISFHTLLHW--------------VSSIESKSSHPMAAALVNHAKLLSTDIKPEKV 480

Query: 481 EEFENFPGEGVRGKIDGKDIYIG----------------KEEMKQGQTLGYVFCEEMAVG 540
           EEFENFPGEGVRGKIDGKDIYIG                +EEMKQGQTLGYVFCEEMAVG
Sbjct: 481 EEFENFPGEGVRGKIDGKDIYIGSTKIAARAGCSSAMKLEEEMKQGQTLGYVFCEEMAVG 540

Query: 541 SFGLSDSCRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEK 600
           SFGLSDSCRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEK
Sbjct: 541 SFGLSDSCRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEK 600

Query: 601 ANVIKEFKRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKI 660
           ANVIKEFKRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKI
Sbjct: 601 ANVIKEFKRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKI 660

Query: 661 PQAIKLARKSHAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLL 720
           PQAIKLARKSHAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLL
Sbjct: 661 PQAIKLARKSHAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLL 720

Query: 721 LRGSDHKHGNKCCKSSKLCSTKHGRCDGSNTRPSHHHHHDHHHHDHRCHVVDDKWPSRDN 780
           LRGSDHKHGNKCCKSSKLCSTKHGRCDGSNTRPSHHHHHDHHHHDHRCHVVDDKWPSRDN
Sbjct: 721 LRGSDHKHGNKCCKSSKLCSTKHGRCDGSNTRPSHHHHHDHHHHDHRCHVVDDKWPSRDN 780

Query: 781 HNGDCGDKFHHNRSNQCEKTPLEKDNNGNCSKKVGEPNCNCHSHHVAIDIHQGIDCERIE 804
           HNGDCGDKFHHNRSNQCEKTPLEKDNNGNCSKKVGEPNCNCHSHHVAIDIHQGIDCERIE
Sbjct: 781 HNGDCGDKFHHNRSNQCEKTPLEKDNNGNCSKKVGEPNCNCHSHHVAIDIHQGIDCERIE 828

BLAST of CmoCh05G007420.1 vs. ExPASy TrEMBL
Match: A0A6J1ENN0 (putative inactive cadmium/zinc-transporting ATPase HMA3 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436150 PE=3 SV=1)

HSP 1 Score: 1428.7 bits (3697), Expect = 0.0e+00
Identity = 758/842 (90.02%), Postives = 767/842 (91.09%), Query Frame = 0

Query: 1   MVGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL 60
           MVGEVAMEKNKK    NKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL
Sbjct: 1   MVGEVAMEKNKK----NKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL 60

Query: 61  I-----------------------ANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY 120
           I                       A VQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY
Sbjct: 61  IVVHDSLLVSQLQIVKALNEARLEAKVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY 120

Query: 121 HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGAMSMNDYMEAGSIVFLF 180
           HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVG MSMNDYMEAGSIVFLF
Sbjct: 121 HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGTMSMNDYMEAGSIVFLF 180

Query: 181 SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPID 240
           SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPID
Sbjct: 181 SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPID 240

Query: 241 GIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF 300
           GIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTT VAEDCVVAKMAEF
Sbjct: 241 GIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTAVAEDCVVAKMAEF 300

Query: 301 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 360
           VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC
Sbjct: 301 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 360

Query: 361 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVIFKLY 420
           PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFV+    
Sbjct: 361 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTDFQ 420

Query: 421 EMISASTPYFIGNCTKNFSPNSVYVEIRVSSIESKSSHPMAAALVNHAKLLSTDIKPEKV 480
            +    + + + +               VSSIESKSSHPMAAALVNHAKLLSTDIKPEKV
Sbjct: 421 ALRDDISFHTLLHW--------------VSSIESKSSHPMAAALVNHAKLLSTDIKPEKV 480

Query: 481 EEFENFPGEGVRGKIDGKDIYIG----------------KEEMKQGQTLGYVFCEEMAVG 540
           EEFENFPGEGVRGKIDGKDIYIG                +EEMK GQTLGYVFCEEMAVG
Sbjct: 481 EEFENFPGEGVRGKIDGKDIYIGSTKIAARAGCSSAMKLEEEMKPGQTLGYVFCEEMAVG 540

Query: 541 SFGLSDSCRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEK 600
           SFGLSDSCRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEK
Sbjct: 541 SFGLSDSCRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEK 600

Query: 601 ANVIKEFKRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKI 660
           AN+IKEFKRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKI
Sbjct: 601 ANMIKEFKRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKI 660

Query: 661 PQAIKLARKSHAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLL 720
           PQAIKLARKSHAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLL
Sbjct: 661 PQAIKLARKSHAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLL 720

Query: 721 LRGSDHKHGNKCCKSSKLCSTKHGRCDGSNTRPSHHHHHDHHHHDHRCHVVDDKWPSRDN 780
           LRGSDHKHGNKCCKSSKLCSTKHGRCDGSNTRPSHHHHHDHHHHDHRCHV+DDK PSRDN
Sbjct: 721 LRGSDHKHGNKCCKSSKLCSTKHGRCDGSNTRPSHHHHHDHHHHDHRCHVIDDKSPSRDN 780

Query: 781 HNGDCGDKFHHNRSNQCEKTPLEKDNNGNCSKKVGEPNCNCHSHHVAIDIHQGIDCERIE 804
           HNGDCGDKFHHNRSNQCEKTPLEKDNNGNCSKKVGEPNCNCHSHHVAIDIHQGIDCERIE
Sbjct: 781 HNGDCGDKFHHNRSNQCEKTPLEKDNNGNCSKKVGEPNCNCHSHHVAIDIHQGIDCERIE 824

BLAST of CmoCh05G007420.1 vs. ExPASy TrEMBL
Match: A0A6J1EUV8 (cadmium/zinc-transporting ATPase HMA2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111436148 PE=3 SV=1)

HSP 1 Score: 1424.5 bits (3686), Expect = 0.0e+00
Identity = 758/842 (90.02%), Postives = 765/842 (90.86%), Query Frame = 0

Query: 1   MVGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL 60
           MVGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL
Sbjct: 1   MVGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL 60

Query: 61  I-----------------------ANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY 120
           I                       ANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY
Sbjct: 61  IVVHDSLLVSQLQIVKALNEARLEANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY 120

Query: 121 HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGAMSMNDYMEAGSIVFLF 180
           HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGAMSMNDYMEAGSIVFLF
Sbjct: 121 HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGAMSMNDYMEAGSIVFLF 180

Query: 181 SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPID 240
           SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPID
Sbjct: 181 SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPID 240

Query: 241 GIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF 300
           GIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF
Sbjct: 241 GIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF 300

Query: 301 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 360
           VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC
Sbjct: 301 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 360

Query: 361 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVIFKLY 420
           PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFV+    
Sbjct: 361 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTDFQ 420

Query: 421 EMISASTPYFIGNCTKNFSPNSVYVEIRVSSIESKSSHPMAAALVNHAKLLSTDIKPEKV 480
            +    + + + +               VSSIESKSSHPMAAALVNHAKLLSTDIKPEKV
Sbjct: 421 ALRDDISFHTLLHW--------------VSSIESKSSHPMAAALVNHAKLLSTDIKPEKV 480

Query: 481 EEFENFPGEGVRGKIDGKDIYIG----------------KEEMKQGQTLGYVFCEEMAVG 540
           EEFENFPGEGVRGKIDGKDIYIG                +EEMKQGQTLGYVFCEEMAVG
Sbjct: 481 EEFENFPGEGVRGKIDGKDIYIGSTKIAARAGCSSAMKLEEEMKQGQTLGYVFCEEMAVG 540

Query: 541 SFGLSDSCRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEK 600
           SFGLSDSCRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEK
Sbjct: 541 SFGLSDSCRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEK 600

Query: 601 ANVIKEFKRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKI 660
           ANVIKEFKRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKI
Sbjct: 601 ANVIKEFKRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKI 660

Query: 661 PQAIKLARKSHAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLL 720
           PQAIKLARKSHAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLL
Sbjct: 661 PQAIKLARKSHAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLL 720

Query: 721 LRGSDHKHGNKCCKSSKLCSTKHGRCDGSNTRPSHHHHHDHHHHDHRCHVVDDKWPSRDN 780
           LRGSDHKHGNKCCKSSKLCSTKHGRCDGSNTRPSHHHHHDHHHHDHRCHVVDDKWPSRDN
Sbjct: 721 LRGSDHKHGNKCCKSSKLCSTKHGRCDGSNTRPSHHHHHDHHHHDHRCHVVDDKWPSRDN 780

Query: 781 HNGDCGDKFHHNRSNQCEKTPLEKDNNGNCSKKVGEPNCNCHSHHVAIDIHQGIDCERIE 804
           HNGDCGDKFHHNRS           NNGNCSKKVGEPNCNCHSHHVAIDIHQGIDCERIE
Sbjct: 781 HNGDCGDKFHHNRS-----------NNGNCSKKVGEPNCNCHSHHVAIDIHQGIDCERIE 817

BLAST of CmoCh05G007420.1 vs. ExPASy TrEMBL
Match: A0A6J1EN92 (putative inactive cadmium/zinc-transporting ATPase HMA3 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111436150 PE=3 SV=1)

HSP 1 Score: 1397.1 bits (3615), Expect = 0.0e+00
Identity = 747/842 (88.72%), Postives = 756/842 (89.79%), Query Frame = 0

Query: 1   MVGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL 60
           MVGEVAMEKNKK    NKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL
Sbjct: 1   MVGEVAMEKNKK----NKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL 60

Query: 61  I-----------------------ANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY 120
           I                       A VQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY
Sbjct: 61  IVVHDSLLVSQLQIVKALNEARLEAKVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY 120

Query: 121 HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGAMSMNDYMEAGSIVFLF 180
           HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVG MSMNDYMEAGSIVFLF
Sbjct: 121 HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGTMSMNDYMEAGSIVFLF 180

Query: 181 SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPID 240
           SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPID
Sbjct: 181 SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPID 240

Query: 241 GIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF 300
           GIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTT VAEDCVVAKMAEF
Sbjct: 241 GIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTAVAEDCVVAKMAEF 300

Query: 301 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 360
           VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC
Sbjct: 301 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 360

Query: 361 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVIFKLY 420
           PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFV+    
Sbjct: 361 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTDFQ 420

Query: 421 EMISASTPYFIGNCTKNFSPNSVYVEIRVSSIESKSSHPMAAALVNHAKLLSTDIKPEKV 480
            +    + + + +               VSSIESKSSHPMAAALVNHAKLLSTDIKPEKV
Sbjct: 421 ALRDDISFHTLLHW--------------VSSIESKSSHPMAAALVNHAKLLSTDIKPEKV 480

Query: 481 EEFENFPGEGVRGKIDGKDIYIG----------------KEEMKQGQTLGYVFCEEMAVG 540
           EEFENFPGEGVRGKIDGKDIYIG                +EEMK GQTLGYVFCEEMAVG
Sbjct: 481 EEFENFPGEGVRGKIDGKDIYIGSTKIAARAGCSSAMKLEEEMKPGQTLGYVFCEEMAVG 540

Query: 541 SFGLSDSCRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEK 600
           SFGLSDSCRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEK
Sbjct: 541 SFGLSDSCRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEK 600

Query: 601 ANVIKEFKRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKI 660
           AN+IKEFKRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKI
Sbjct: 601 ANMIKEFKRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKI 660

Query: 661 PQAIKLARKSHAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLL 720
           PQAIKLARKSHAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLL
Sbjct: 661 PQAIKLARKSHAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLL 720

Query: 721 LRGSDHKHGNKCCKSSKLCSTKHGRCDGSNTRPSHHHHHDHHHHDHRCHVVDDKWPSRDN 780
           LRGSDHKHGNKCCKSSKLCSTKHGRCDGSNTRPSHHHHHDHHHHDHRCHV+DDK PSRDN
Sbjct: 721 LRGSDHKHGNKCCKSSKLCSTKHGRCDGSNTRPSHHHHHDHHHHDHRCHVIDDKSPSRDN 780

Query: 781 HNGDCGDKFHHNRSNQCEKTPLEKDNNGNCSKKVGEPNCNCHSHHVAIDIHQGIDCERIE 804
           HNGDCGDKFHHNRS           NNGNCSKKVGEPNCNCHSHHVAIDIHQGIDCERIE
Sbjct: 781 HNGDCGDKFHHNRS-----------NNGNCSKKVGEPNCNCHSHHVAIDIHQGIDCERIE 813

BLAST of CmoCh05G007420.1 vs. ExPASy TrEMBL
Match: A0A6J1K4T4 (putative inactive cadmium/zinc-transporting ATPase HMA3 OS=Cucurbita maxima OX=3661 GN=LOC111492308 PE=3 SV=1)

HSP 1 Score: 1385.2 bits (3584), Expect = 0.0e+00
Identity = 739/843 (87.66%), Postives = 755/843 (89.56%), Query Frame = 0

Query: 1   MVGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL 60
           MVGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPL+ENILKPLEGIKQISVIVPTRTL
Sbjct: 1   MVGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLVENILKPLEGIKQISVIVPTRTL 60

Query: 61  I-----------------------ANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY 120
           I                       ANVQ+ GKGISKKKWPSPFAIASGLLLTASFLKYVY
Sbjct: 61  IVVHDSLLVSQLQIVKALNEARLEANVQVNGKGISKKKWPSPFAIASGLLLTASFLKYVY 120

Query: 121 HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGAMSMNDYMEAGSIVFLF 180
           HPLRWLA+AAVAAGIFPILLKAISAVRHLRID NILAI+AVVG +SMNDYMEAGSIVFLF
Sbjct: 121 HPLRWLAIAAVAAGIFPILLKAISAVRHLRIDANILAIVAVVGTISMNDYMEAGSIVFLF 180

Query: 181 SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPID 240
           SIAEWLESRASHKANAAM SLMSLAPQKA IAETGEVV VKEV LKSVLAVKGGEVIPID
Sbjct: 181 SIAEWLESRASHKANAAMSSLMSLAPQKAIIAETGEVVAVKEVELKSVLAVKGGEVIPID 240

Query: 241 GIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF 300
           GIVVDGKCDVDEK+LTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF
Sbjct: 241 GIVVDGKCDVDEKTLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF 300

Query: 301 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 360
           VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC
Sbjct: 301 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 360

Query: 361 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVIFKLY 420
           PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFV+    
Sbjct: 361 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVV---- 420

Query: 421 EMISASTPYFIGNCTKNFSPNSVYVEIRVSSIESKSSHPMAAALVNHAKLLSTDIKPEKV 480
                 T +       NF     +    VSSIESKSSHPMAAALVNHAKLLS DIKPEKV
Sbjct: 421 ------TNFQALRDDINFHTLLHW----VSSIESKSSHPMAAALVNHAKLLSIDIKPEKV 480

Query: 481 EEFENFPGEGVRGKIDGKDIYIGK----------------EEMKQGQTLGYVFCEEMAVG 540
           EEFENFPGEGVRGKIDGKDIYIG                 EEMKQGQTLGYVFC EMAVG
Sbjct: 481 EEFENFPGEGVRGKIDGKDIYIGSTKIAARAGCSSAMKLDEEMKQGQTLGYVFCGEMAVG 540

Query: 541 SFGLSDSCRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEK 600
           +FGLSDSCRSGAKE ME+LKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEK
Sbjct: 541 AFGLSDSCRSGAKEAMEQLKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEK 600

Query: 601 ANVIKEFKRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKI 660
           AN+IKEFKRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKI
Sbjct: 601 ANMIKEFKRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKI 660

Query: 661 PQAIKLARKSHAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLL 720
           PQAIKLARKSHAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLL
Sbjct: 661 PQAIKLARKSHAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLL 720

Query: 721 LRGSDHKHGNKCCKSSKLCSTKHGRCDGSNTRPSHHHHHDHHHHDHRCHVVDDKWPSRDN 780
           LRGSDHKHGNKCCKSSKLCSTKHGRCDGSNTRPSHHHHH HHHH HRCHVVDDK PSRDN
Sbjct: 721 LRGSDHKHGNKCCKSSKLCSTKHGRCDGSNTRPSHHHHHGHHHHSHRCHVVDDKSPSRDN 780

Query: 781 HNGDCGDKFHHNRSNQCEKTPLEKDNNGNCSKKVGEPNCNCHS-HHVAIDIHQGIDCERI 804
           HN DCGDKFHHN SNQCEK P EK+NNGNCS KV EPNCNCHS HHVAIDIH+G DCER+
Sbjct: 781 HNRDCGDKFHHNDSNQCEKNPHEKENNGNCSTKVDEPNCNCHSHHHVAIDIHEGTDCERV 829

BLAST of CmoCh05G007420.1 vs. NCBI nr
Match: XP_022929619.1 (cadmium/zinc-transporting ATPase HMA2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1456.4 bits (3769), Expect = 0.0e+00
Identity = 769/842 (91.33%), Postives = 776/842 (92.16%), Query Frame = 0

Query: 1   MVGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL 60
           MVGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL
Sbjct: 1   MVGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL 60

Query: 61  I-----------------------ANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY 120
           I                       ANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY
Sbjct: 61  IVVHDSLLVSQLQIVKALNEARLEANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY 120

Query: 121 HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGAMSMNDYMEAGSIVFLF 180
           HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGAMSMNDYMEAGSIVFLF
Sbjct: 121 HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGAMSMNDYMEAGSIVFLF 180

Query: 181 SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPID 240
           SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPID
Sbjct: 181 SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPID 240

Query: 241 GIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF 300
           GIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF
Sbjct: 241 GIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF 300

Query: 301 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 360
           VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC
Sbjct: 301 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 360

Query: 361 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVIFKLY 420
           PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFV+    
Sbjct: 361 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTDFQ 420

Query: 421 EMISASTPYFIGNCTKNFSPNSVYVEIRVSSIESKSSHPMAAALVNHAKLLSTDIKPEKV 480
            +    + + + +               VSSIESKSSHPMAAALVNHAKLLSTDIKPEKV
Sbjct: 421 ALRDDISFHTLLHW--------------VSSIESKSSHPMAAALVNHAKLLSTDIKPEKV 480

Query: 481 EEFENFPGEGVRGKIDGKDIYIG----------------KEEMKQGQTLGYVFCEEMAVG 540
           EEFENFPGEGVRGKIDGKDIYIG                +EEMKQGQTLGYVFCEEMAVG
Sbjct: 481 EEFENFPGEGVRGKIDGKDIYIGSTKIAARAGCSSAMKLEEEMKQGQTLGYVFCEEMAVG 540

Query: 541 SFGLSDSCRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEK 600
           SFGLSDSCRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEK
Sbjct: 541 SFGLSDSCRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEK 600

Query: 601 ANVIKEFKRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKI 660
           ANVIKEFKRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKI
Sbjct: 601 ANVIKEFKRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKI 660

Query: 661 PQAIKLARKSHAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLL 720
           PQAIKLARKSHAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLL
Sbjct: 661 PQAIKLARKSHAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLL 720

Query: 721 LRGSDHKHGNKCCKSSKLCSTKHGRCDGSNTRPSHHHHHDHHHHDHRCHVVDDKWPSRDN 780
           LRGSDHKHGNKCCKSSKLCSTKHGRCDGSNTRPSHHHHHDHHHHDHRCHVVDDKWPSRDN
Sbjct: 721 LRGSDHKHGNKCCKSSKLCSTKHGRCDGSNTRPSHHHHHDHHHHDHRCHVVDDKWPSRDN 780

Query: 781 HNGDCGDKFHHNRSNQCEKTPLEKDNNGNCSKKVGEPNCNCHSHHVAIDIHQGIDCERIE 804
           HNGDCGDKFHHNRSNQCEKTPLEKDNNGNCSKKVGEPNCNCHSHHVAIDIHQGIDCERIE
Sbjct: 781 HNGDCGDKFHHNRSNQCEKTPLEKDNNGNCSKKVGEPNCNCHSHHVAIDIHQGIDCERIE 828

BLAST of CmoCh05G007420.1 vs. NCBI nr
Match: XP_022929621.1 (putative inactive cadmium/zinc-transporting ATPase HMA3 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1428.7 bits (3697), Expect = 0.0e+00
Identity = 758/842 (90.02%), Postives = 767/842 (91.09%), Query Frame = 0

Query: 1   MVGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL 60
           MVGEVAMEKNKK    NKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL
Sbjct: 1   MVGEVAMEKNKK----NKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL 60

Query: 61  I-----------------------ANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY 120
           I                       A VQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY
Sbjct: 61  IVVHDSLLVSQLQIVKALNEARLEAKVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY 120

Query: 121 HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGAMSMNDYMEAGSIVFLF 180
           HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVG MSMNDYMEAGSIVFLF
Sbjct: 121 HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGTMSMNDYMEAGSIVFLF 180

Query: 181 SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPID 240
           SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPID
Sbjct: 181 SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPID 240

Query: 241 GIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF 300
           GIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTT VAEDCVVAKMAEF
Sbjct: 241 GIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTAVAEDCVVAKMAEF 300

Query: 301 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 360
           VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC
Sbjct: 301 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 360

Query: 361 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVIFKLY 420
           PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFV+    
Sbjct: 361 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTDFQ 420

Query: 421 EMISASTPYFIGNCTKNFSPNSVYVEIRVSSIESKSSHPMAAALVNHAKLLSTDIKPEKV 480
            +    + + + +               VSSIESKSSHPMAAALVNHAKLLSTDIKPEKV
Sbjct: 421 ALRDDISFHTLLHW--------------VSSIESKSSHPMAAALVNHAKLLSTDIKPEKV 480

Query: 481 EEFENFPGEGVRGKIDGKDIYIG----------------KEEMKQGQTLGYVFCEEMAVG 540
           EEFENFPGEGVRGKIDGKDIYIG                +EEMK GQTLGYVFCEEMAVG
Sbjct: 481 EEFENFPGEGVRGKIDGKDIYIGSTKIAARAGCSSAMKLEEEMKPGQTLGYVFCEEMAVG 540

Query: 541 SFGLSDSCRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEK 600
           SFGLSDSCRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEK
Sbjct: 541 SFGLSDSCRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEK 600

Query: 601 ANVIKEFKRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKI 660
           AN+IKEFKRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKI
Sbjct: 601 ANMIKEFKRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKI 660

Query: 661 PQAIKLARKSHAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLL 720
           PQAIKLARKSHAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLL
Sbjct: 661 PQAIKLARKSHAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLL 720

Query: 721 LRGSDHKHGNKCCKSSKLCSTKHGRCDGSNTRPSHHHHHDHHHHDHRCHVVDDKWPSRDN 780
           LRGSDHKHGNKCCKSSKLCSTKHGRCDGSNTRPSHHHHHDHHHHDHRCHV+DDK PSRDN
Sbjct: 721 LRGSDHKHGNKCCKSSKLCSTKHGRCDGSNTRPSHHHHHDHHHHDHRCHVIDDKSPSRDN 780

Query: 781 HNGDCGDKFHHNRSNQCEKTPLEKDNNGNCSKKVGEPNCNCHSHHVAIDIHQGIDCERIE 804
           HNGDCGDKFHHNRSNQCEKTPLEKDNNGNCSKKVGEPNCNCHSHHVAIDIHQGIDCERIE
Sbjct: 781 HNGDCGDKFHHNRSNQCEKTPLEKDNNGNCSKKVGEPNCNCHSHHVAIDIHQGIDCERIE 824

BLAST of CmoCh05G007420.1 vs. NCBI nr
Match: XP_022929620.1 (cadmium/zinc-transporting ATPase HMA2-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1424.5 bits (3686), Expect = 0.0e+00
Identity = 758/842 (90.02%), Postives = 765/842 (90.86%), Query Frame = 0

Query: 1   MVGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL 60
           MVGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL
Sbjct: 1   MVGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL 60

Query: 61  I-----------------------ANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY 120
           I                       ANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY
Sbjct: 61  IVVHDSLLVSQLQIVKALNEARLEANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY 120

Query: 121 HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGAMSMNDYMEAGSIVFLF 180
           HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGAMSMNDYMEAGSIVFLF
Sbjct: 121 HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGAMSMNDYMEAGSIVFLF 180

Query: 181 SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPID 240
           SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPID
Sbjct: 181 SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPID 240

Query: 241 GIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF 300
           GIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF
Sbjct: 241 GIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF 300

Query: 301 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 360
           VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC
Sbjct: 301 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 360

Query: 361 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVIFKLY 420
           PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFV+    
Sbjct: 361 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTDFQ 420

Query: 421 EMISASTPYFIGNCTKNFSPNSVYVEIRVSSIESKSSHPMAAALVNHAKLLSTDIKPEKV 480
            +    + + + +               VSSIESKSSHPMAAALVNHAKLLSTDIKPEKV
Sbjct: 421 ALRDDISFHTLLHW--------------VSSIESKSSHPMAAALVNHAKLLSTDIKPEKV 480

Query: 481 EEFENFPGEGVRGKIDGKDIYIG----------------KEEMKQGQTLGYVFCEEMAVG 540
           EEFENFPGEGVRGKIDGKDIYIG                +EEMKQGQTLGYVFCEEMAVG
Sbjct: 481 EEFENFPGEGVRGKIDGKDIYIGSTKIAARAGCSSAMKLEEEMKQGQTLGYVFCEEMAVG 540

Query: 541 SFGLSDSCRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEK 600
           SFGLSDSCRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEK
Sbjct: 541 SFGLSDSCRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEK 600

Query: 601 ANVIKEFKRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKI 660
           ANVIKEFKRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKI
Sbjct: 601 ANVIKEFKRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKI 660

Query: 661 PQAIKLARKSHAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLL 720
           PQAIKLARKSHAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLL
Sbjct: 661 PQAIKLARKSHAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLL 720

Query: 721 LRGSDHKHGNKCCKSSKLCSTKHGRCDGSNTRPSHHHHHDHHHHDHRCHVVDDKWPSRDN 780
           LRGSDHKHGNKCCKSSKLCSTKHGRCDGSNTRPSHHHHHDHHHHDHRCHVVDDKWPSRDN
Sbjct: 721 LRGSDHKHGNKCCKSSKLCSTKHGRCDGSNTRPSHHHHHDHHHHDHRCHVVDDKWPSRDN 780

Query: 781 HNGDCGDKFHHNRSNQCEKTPLEKDNNGNCSKKVGEPNCNCHSHHVAIDIHQGIDCERIE 804
           HNGDCGDKFHHNRS           NNGNCSKKVGEPNCNCHSHHVAIDIHQGIDCERIE
Sbjct: 781 HNGDCGDKFHHNRS-----------NNGNCSKKVGEPNCNCHSHHVAIDIHQGIDCERIE 817

BLAST of CmoCh05G007420.1 vs. NCBI nr
Match: XP_022929622.1 (putative inactive cadmium/zinc-transporting ATPase HMA3 isoform X2 [Cucurbita moschata])

HSP 1 Score: 1397.1 bits (3615), Expect = 0.0e+00
Identity = 747/842 (88.72%), Postives = 756/842 (89.79%), Query Frame = 0

Query: 1   MVGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL 60
           MVGEVAMEKNKK    NKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL
Sbjct: 1   MVGEVAMEKNKK----NKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL 60

Query: 61  I-----------------------ANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY 120
           I                       A VQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY
Sbjct: 61  IVVHDSLLVSQLQIVKALNEARLEAKVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY 120

Query: 121 HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGAMSMNDYMEAGSIVFLF 180
           HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVG MSMNDYMEAGSIVFLF
Sbjct: 121 HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGTMSMNDYMEAGSIVFLF 180

Query: 181 SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPID 240
           SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPID
Sbjct: 181 SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPID 240

Query: 241 GIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF 300
           GIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTT VAEDCVVAKMAEF
Sbjct: 241 GIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTAVAEDCVVAKMAEF 300

Query: 301 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 360
           VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC
Sbjct: 301 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 360

Query: 361 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVIFKLY 420
           PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFV+    
Sbjct: 361 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTDFQ 420

Query: 421 EMISASTPYFIGNCTKNFSPNSVYVEIRVSSIESKSSHPMAAALVNHAKLLSTDIKPEKV 480
            +    + + + +               VSSIESKSSHPMAAALVNHAKLLSTDIKPEKV
Sbjct: 421 ALRDDISFHTLLHW--------------VSSIESKSSHPMAAALVNHAKLLSTDIKPEKV 480

Query: 481 EEFENFPGEGVRGKIDGKDIYIG----------------KEEMKQGQTLGYVFCEEMAVG 540
           EEFENFPGEGVRGKIDGKDIYIG                +EEMK GQTLGYVFCEEMAVG
Sbjct: 481 EEFENFPGEGVRGKIDGKDIYIGSTKIAARAGCSSAMKLEEEMKPGQTLGYVFCEEMAVG 540

Query: 541 SFGLSDSCRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEK 600
           SFGLSDSCRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEK
Sbjct: 541 SFGLSDSCRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEK 600

Query: 601 ANVIKEFKRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKI 660
           AN+IKEFKRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKI
Sbjct: 601 ANMIKEFKRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKI 660

Query: 661 PQAIKLARKSHAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLL 720
           PQAIKLARKSHAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLL
Sbjct: 661 PQAIKLARKSHAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLL 720

Query: 721 LRGSDHKHGNKCCKSSKLCSTKHGRCDGSNTRPSHHHHHDHHHHDHRCHVVDDKWPSRDN 780
           LRGSDHKHGNKCCKSSKLCSTKHGRCDGSNTRPSHHHHHDHHHHDHRCHV+DDK PSRDN
Sbjct: 721 LRGSDHKHGNKCCKSSKLCSTKHGRCDGSNTRPSHHHHHDHHHHDHRCHVIDDKSPSRDN 780

Query: 781 HNGDCGDKFHHNRSNQCEKTPLEKDNNGNCSKKVGEPNCNCHSHHVAIDIHQGIDCERIE 804
           HNGDCGDKFHHNRS           NNGNCSKKVGEPNCNCHSHHVAIDIHQGIDCERIE
Sbjct: 781 HNGDCGDKFHHNRS-----------NNGNCSKKVGEPNCNCHSHHVAIDIHQGIDCERIE 813

BLAST of CmoCh05G007420.1 vs. NCBI nr
Match: KAG6598888.1 (Cadmium/zinc-transporting ATPase HMA2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1392.9 bits (3604), Expect = 0.0e+00
Identity = 747/843 (88.61%), Postives = 755/843 (89.56%), Query Frame = 0

Query: 1   MVGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL 60
           MVGEVAMEKNKKV RENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL
Sbjct: 1   MVGEVAMEKNKKVTRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL 60

Query: 61  I-----------------------ANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY 120
           I                       ANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY
Sbjct: 61  IVVHDSLLVSQLQIVKALNEARLEANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY 120

Query: 121 HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGAMSMNDYMEAGSIVFLF 180
           HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVG MSMNDYME GSIVFLF
Sbjct: 121 HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGTMSMNDYMEGGSIVFLF 180

Query: 181 SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPID 240
           SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEV LKSVLAVKGGEVIPID
Sbjct: 181 SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVKLKSVLAVKGGEVIPID 240

Query: 241 GIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF 300
           GIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF
Sbjct: 241 GIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF 300

Query: 301 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 360
           VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC
Sbjct: 301 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 360

Query: 361 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVIFKLY 420
           PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFV+    
Sbjct: 361 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTDFQ 420

Query: 421 EMISASTPYFIGNCTKNFSPNSVYVEIRVSSIESKSSHPMAAALVNHAKLLSTDIKPEKV 480
            +    + + + +               VSSIESKSSHPMAAALVNHAKLLS DIKPEKV
Sbjct: 421 ALRDDISFHTLLHW--------------VSSIESKSSHPMAAALVNHAKLLSIDIKPEKV 480

Query: 481 EEFENFPGEGVRGKIDGKDIYIG----------------KEEMKQGQTLGYVFCEEMAVG 540
           EEFENFPGEGVRGKIDGKDIYIG                +EEMKQGQTLGYVFCEEMAVG
Sbjct: 481 EEFENFPGEGVRGKIDGKDIYIGSTKIAARAGCSSAMKLEEEMKQGQTLGYVFCEEMAVG 540

Query: 541 SFGLSDSCRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEK 600
           SFGLSDSCRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEK
Sbjct: 541 SFGLSDSCRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEK 600

Query: 601 ANVIKEFKRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKI 660
           ANVIKEFKRDYG TAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKI
Sbjct: 601 ANVIKEFKRDYGGTAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKI 660

Query: 661 PQAIKLARKSHAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLL 720
           PQAIKLARKSHAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLL
Sbjct: 661 PQAIKLARKSHAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLL 720

Query: 721 LRGSDHKHGNKCCKSSKLCSTKHGRCDGSNTRPS-HHHHHDHHHHDHRCHVVDDKWPSRD 780
           LRGSDHKHGNKCCKSSKLCSTKHGRCDGSNTRPS HHHHHDHHHH HRCHVVDDK PSRD
Sbjct: 721 LRGSDHKHGNKCCKSSKLCSTKHGRCDGSNTRPSHHHHHHDHHHHGHRCHVVDDKSPSRD 780

Query: 781 NHNGDCGDKFHHNRSNQCEKTPLEKDNNGNCSKKVGEPNCNCHSHHVAIDIHQGIDCERI 804
           NHNGD GDKFHHN S           NNGNCSKKVGEPNCNCHSHHVAIDIH+GIDCERI
Sbjct: 781 NHNGDFGDKFHHNHS-----------NNGNCSKKVGEPNCNCHSHHVAIDIHEGIDCERI 818

BLAST of CmoCh05G007420.1 vs. TAIR 10
Match: AT4G30110.1 (heavy metal atpase 2 )

HSP 1 Score: 856.7 bits (2212), Expect = 1.5e-248
Identity = 452/723 (62.52%), Postives = 554/723 (76.63%), Query Frame = 0

Query: 18  KFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTLI---------------- 77
           K  +S+FDVLGICC+SEVPLIENIL  ++G+K+ SVIVP+RT+I                
Sbjct: 5   KMTKSYFDVLGICCTSEVPLIENILNSMDGVKEFSVIVPSRTVIVVHDTLILSQFQIVKA 64

Query: 78  -------ANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVYHPLRWLAVAAVAAGIFP 137
                  ANV++ G+   K KWPSPFA+ SG+LL  SF KY+Y P RWLAVAAV AGI+P
Sbjct: 65  LNQAQLEANVRVTGETNFKNKWPSPFAVVSGILLLLSFFKYLYSPFRWLAVAAVVAGIYP 124

Query: 138 ILLKAISAVRHLRIDVNILAIIAVVGAMSMNDYMEAGSIVFLFSIAEWLESRASHKANAA 197
           IL KA++++   RID+NIL ++ V   + M DY EA  +VFLF+IAEWL+SRAS+KA+A 
Sbjct: 125 ILAKAVASLARFRIDINILVVVTVGATIGMQDYTEAAVVVFLFTIAEWLQSRASYKASAV 184

Query: 198 MWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPIDGIVVDGKCDVDEKSLTG 257
           M SLMSLAPQKA IAETGE VEV E+   +V+AVK GE IPIDG+VVDG C+VDEK+LTG
Sbjct: 185 MQSLMSLAPQKAVIAETGEEVEVDELKTNTVIAVKAGETIPIDGVVVDGNCEVDEKTLTG 244

Query: 258 ETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEFVEEAQNNKSKTQRFIDE 317
           E FPVPKLKDS VWAGTINLNGYI+V TT +AEDCVVAKMA+ VEEAQN+K++TQRFID+
Sbjct: 245 EAFPVPKLKDSTVWAGTINLNGYITVNTTALAEDCVVAKMAKLVEEAQNSKTETQRFIDK 304

Query: 318 CAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSACPCALILSTPVAAFCALT 377
           C+KYYTPA+++IS C  AIP A +VHNL HW HLALVVLVSACPC LILSTPVA FCALT
Sbjct: 305 CSKYYTPAIILISICFVAIPFALKVHNLKHWVHLALVVLVSACPCGLILSTPVATFCALT 364

Query: 378 KAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVIFKLYEMISASTPYFIGNCTKN 437
           KAA +G+LIKG+D+LE LAKIK+VAFDKTGTITRGEF++     +            +++
Sbjct: 365 KAATSGLLIKGADYLETLAKIKIVAFDKTGTITRGEFIVMDFQSL------------SED 424

Query: 438 FSPNSVYVEIRVSSIESKSSHPMAAALVNHAKLLSTDIKPEKVEEFENFPGEGVRGKIDG 497
            S  S+     VSS ESKSSHPMAAA+V++A+ +S + KPE VE+++NFPGEG+ GKIDG
Sbjct: 425 ISLQSLL--YWVSSTESKSSHPMAAAVVDYARSVSVEPKPEAVEDYQNFPGEGIYGKIDG 484

Query: 498 KDIYIGKE----------------EMKQGQTLGYVFCEEMAVGSFGLSDSCRSGAKEGME 557
           K++YIG +                + K G+T+GYV+  E   G F LSD+CRSG  + M+
Sbjct: 485 KEVYIGNKRIASRAGCLSVPDIDVDTKGGKTIGYVYVGETLAGVFNLSDACRSGVAQAMK 544

Query: 558 ELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRDYGATAMV 617
           ELKSLGIK AMLTGD  AAAMHAQ+QL NA+D++ +ELLP++K+ +IK+ KR+ G TAMV
Sbjct: 545 ELKSLGIKIAMLTGDNHAAAMHAQEQLGNAMDIVRAELLPEDKSEIIKQLKREEGPTAMV 604

Query: 618 GDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKVVQN 677
           GDGLND PALATADIGISMG+SGS LAT+TGN+ILMSNDI +IPQAIKLA+++  KVV+N
Sbjct: 605 GDGLNDAPALATADIGISMGVSGSALATETGNIILMSNDIRRIPQAIKLAKRAKRKVVEN 664

Query: 678 VILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCKSSK 702
           V++SIT K AIL LA AGHPL+WAAVLADVGTCLLVILNSMLLL    HK GNKC + S 
Sbjct: 665 VVISITMKGAILALAFAGHPLIWAAVLADVGTCLLVILNSMLLL-SDKHKTGNKCYRESS 712

BLAST of CmoCh05G007420.1 vs. TAIR 10
Match: AT2G19110.1 (heavy metal atpase 4 )

HSP 1 Score: 828.2 bits (2138), Expect = 5.8e-240
Identity = 474/823 (57.59%), Postives = 596/823 (72.42%), Query Frame = 0

Query: 8   EKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTLI------ 67
           E+ KKV+   K ++S+FDVLGICC+SEVP+IENILK L+G+K+ SVIVP+RT+I      
Sbjct: 8   EEKKKVK---KLQKSYFDVLGICCTSEVPIIENILKSLDGVKEYSVIVPSRTVIVVHDSL 67

Query: 68  -----------------ANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVYHPLRWLA 127
                            ANV++ G+   K KWPSPFA+ SGLLL  SFLK+VY PLRWLA
Sbjct: 68  LISPFQIAKALNEARLEANVRVNGETSFKNKWPSPFAVVSGLLLLLSFLKFVYSPLRWLA 127

Query: 128 VAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGAMSMNDYMEAGSIVFLFSIAEWLE 187
           VAAVAAGI+PIL KA ++++  RID+NIL II V+  ++M D+MEA ++VFLF+I++WLE
Sbjct: 128 VAAVAAGIYPILAKAFASIKRPRIDINILVIITVIATLAMQDFMEAAAVVFLFTISDWLE 187

Query: 188 SRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPIDGIVVDGK 247
           +RAS+KA + M SLMSLAPQKA IAETGE VEV EV + +V+AVK GE IPIDGIVVDG 
Sbjct: 188 TRASYKATSVMQSLMSLAPQKAIIAETGEEVEVDEVKVDTVVAVKAGETIPIDGIVVDGN 247

Query: 248 CDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEFVEEAQNN 307
           C+VDEK+LTGE FPVPK +DS VWAGTINLNGYI V+TT +A DCVVAKMA+ VEEAQ++
Sbjct: 248 CEVDEKTLTGEAFPVPKQRDSTVWAGTINLNGYICVKTTSLAGDCVVAKMAKLVEEAQSS 307

Query: 308 KSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSACPCALILS 367
           K+K+QR ID+C++YYTPA++++SACVA +P   +VHNL HWFHLALVVLVS CPC LILS
Sbjct: 308 KTKSQRLIDKCSQYYTPAIILVSACVAIVPVIMKVHNLKHWFHLALVVLVSGCPCGLILS 367

Query: 368 TPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVIFKLYEMISAST 427
           TPVA FCALTKAA +G+LIK +D+L+ L+KIK+VAFDKTGTITRGEF++     +     
Sbjct: 368 TPVATFCALTKAATSGLLIKSADYLDTLSKIKIVAFDKTGTITRGEFIVIDFKSL----- 427

Query: 428 PYFIGNCTKNFSPNSVYVEIRVSSIESKSSHPMAAALVNHAKLLSTDIKPEKVEEFENFP 487
                  +++ +  S+     VSS+ESKSSHPMAA +V++AK +S + +PE+VE+++NFP
Sbjct: 428 -------SRDINLRSLL--YWVSSVESKSSHPMAATIVDYAKSVSVEPRPEEVEDYQNFP 487

Query: 488 GEGVRGKIDGKDIYIGKEEM----------------KQGQTLGYVFCEEMAVGSFGLSDS 547
           GEG+ GKIDG DI+IG +++                K G+T+GYV+  E   G F LSD+
Sbjct: 488 GEGIYGKIDGNDIFIGNKKIASRAGCSTVPEIEVDTKGGKTVGYVYVGERLAGFFNLSDA 547

Query: 548 CRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEF 607
           CRSG  + M ELKSLGIKTAMLTGD  AAAMHAQ+QL N +DV+H +LLP++K+ +I+EF
Sbjct: 548 CRSGVSQAMAELKSLGIKTAMLTGDNQAAAMHAQEQLGNVLDVVHGDLLPEDKSRIIQEF 607

Query: 608 KRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLA 667
           K++ G TAMVGDG+ND PALATADIGISMGISGS LATQTGN+ILMSNDI +IPQA+KLA
Sbjct: 608 KKE-GPTAMVGDGVNDAPALATADIGISMGISGSALATQTGNIILMSNDIRRIPQAVKLA 667

Query: 668 RKSHAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHK 727
           R++  KVV+NV LSI  K  IL LA AGHPL+WAAVL DVGTCLLVI NSMLLLR    K
Sbjct: 668 RRARRKVVENVCLSIILKAGILALAFAGHPLIWAAVLVDVGTCLLVIFNSMLLLR-EKKK 727

Query: 728 HGNKCC---KSSKLCSTK-HGRCD-------GSNTRPSHHHHHDHHHHD--HRCHVV--- 775
            GNK C    +SKL   K  G  D       G  T+  +         D  ++ +VV   
Sbjct: 728 IGNKKCYRASTSKLNGRKLEGDDDYVVDLEAGLLTKSGNGQCKSSCCGDKKNQENVVMMK 787

BLAST of CmoCh05G007420.1 vs. TAIR 10
Match: AT4G30120.1 (heavy metal atpase 3 )

HSP 1 Score: 554.7 bits (1428), Expect = 1.2e-157
Identity = 300/558 (53.76%), Postives = 388/558 (69.53%), Query Frame = 0

Query: 8   EKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTLI------ 67
           E++KK+      + S+FDV+GICCSSEV ++ N+L+ ++G+K+ SVIVP+RT+I      
Sbjct: 5   EESKKM----NLQTSYFDVVGICCSSEVSIVGNVLRQVDGVKEFSVIVPSRTVIVVHDTF 64

Query: 68  -----------------ANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVYHPLRWLA 127
                            A+V+  G+   K +WPSPFAI SG+LL  SF KY Y PL WLA
Sbjct: 65  LISPLQIVKALNQARLEASVRPYGETSLKSQWPSPFAIVSGVLLVLSFFKYFYSPLEWLA 124

Query: 128 VAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGAMSMNDYMEAGSIVFLFSIAEWLE 187
           + AV AG+FPIL KA+++V   R+D+N L +IAV+  + M D+ EA +IVFLFS+A+WLE
Sbjct: 125 IVAVVAGVFPILAKAVASVTRFRLDINALTLIAVIATLCMQDFTEAATIVFLFSVADWLE 184

Query: 188 SRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPIDGIVVDGK 247
           S A+HKA+  M SLMSLAP+KA IA+TG  V+V EV + +V++VK GE IPIDG+VVDG 
Sbjct: 185 SSAAHKASIVMSSLMSLAPRKAVIADTGLEVDVDEVGINTVVSVKAGESIPIDGVVVDGS 244

Query: 248 CDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEFVEEAQNN 307
           CDVDEK+LTGE+FPV K ++S V A TINLNGYI V+TT +A DCVVAKM + VEEAQ +
Sbjct: 245 CDVDEKTLTGESFPVSKQRESTVMAATINLNGYIKVKTTALARDCVVAKMTKLVEEAQKS 304

Query: 308 KSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSACPCALILS 367
           ++KTQRFID+C++YYTPAVVV +AC A IP   +V +LSHWFHLALVVLVS CPC LILS
Sbjct: 305 QTKTQRFIDKCSRYYTPAVVVSAACFAVIPVLLKVQDLSHWFHLALVVLVSGCPCGLILS 364

Query: 368 TPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVIFKLYEMISAST 427
           TPVA FCALTKAA +G LIK  D LE LAKIK+VAFDKTGTIT+ EF++           
Sbjct: 365 TPVATFCALTKAATSGFLIKTGDCLETLAKIKIVAFDKTGTITKAEFMVSDF-------- 424

Query: 428 PYFIGNCTKNFSP--NSVYVEIRVSSIESKSSHPMAAALVNHAKLLSTDIKPEKVEEFEN 487
                   ++ SP  N   +   VSSIE KSSHPMAAAL+++A+ +S + KP+ VE F+N
Sbjct: 425 --------RSLSPSINLHKLLYWVSSIECKSSHPMAAALIDYARSVSVEPKPDIVENFQN 484

Query: 488 FPGEGVRGKIDGKDIYIGKEE------------------MKQGQTLGYVFCEEMAVGSFG 523
           FPGEGV G+IDG+DIYIG +                   MK+G+T+GY++      GSF 
Sbjct: 485 FPGEGVYGRIDGQDIYIGNKRIAQRAGCLTDNVPDIEATMKRGKTIGYIYMGAKLTGSFN 542

BLAST of CmoCh05G007420.1 vs. TAIR 10
Match: AT5G44790.1 (copper-exporting ATPase / responsive-to-antagonist 1 / copper-transporting ATPase (RAN1) )

HSP 1 Score: 218.0 bits (554), Expect = 2.7e-56
Identity = 186/622 (29.90%), Postives = 307/622 (49.36%), Query Frame = 0

Query: 130 VNILAIIAVVGAMSMNDYMEAGSIVFLFSIAEWLESRASHKANAAMWSLMSLAPQKA--- 189
           V  L   AV G  S   +  +  ++    + ++LES A  K + AM  L+ L P  A   
Sbjct: 386 VGALLYGAVTGFWSPTYFDASAMLITFVLLGKYLESLAKGKTSDAMKKLVQLTPATAILL 445

Query: 190 TIAETGEVVEVKEVTLKSV-----LAVKGGEVIPIDGIVVDGKCDVDEKSLTGETFPVPK 249
           T  + G++V  +E+    +     L V  G  IP DG+VV G   V+E  +TGE+ PV K
Sbjct: 446 TEGKGGKLVGEREIDALLIQPGDTLKVHPGAKIPADGVVVWGSSYVNESMVTGESVPVSK 505

Query: 250 LKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEFVEEAQNNKSKTQRFIDECAKYYTP 309
             DS V  GTIN++G + ++ T V  D V++++   VE AQ +K+  Q+F D  A  + P
Sbjct: 506 EVDSPVIGGTINMHGALHMKATKVGSDAVLSQIISLVETAQMSKAPIQKFADYVASIFVP 565

Query: 310 AVVV----------ISACVAAIPAAFRVHNLSHW---FHLALVVLVSACPCALILSTPVA 369
            V+           I   V A P  +   N +H+      ++ V+V ACPCAL L+TP A
Sbjct: 566 VVITLALFTLVGWSIGGAVGAYPDEWLPENGTHFVFSLMFSISVVVIACPCALGLATPTA 625

Query: 370 AFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVIFKLYEMISASTPYFI 429
              A    A  GVLIKG D LE   K+K V FDKTGT+T+G+  +               
Sbjct: 626 VMVATGVGATNGVLIKGGDALEKAHKVKYVIFDKTGTLTQGKATV--------------- 685

Query: 430 GNCTKNFS--PNSVYVEIRVSSIESKSSHPMAAALVNHAKLL-------------STDIK 489
              TK FS      ++ + V+S E+ S HP+A A+V +A+               + D++
Sbjct: 686 -TTTKVFSEMDRGEFLTL-VASAEASSEHPLAKAIVAYARHFHFFDESTEDGETNNKDLQ 745

Query: 490 PE----KVEEFENFPGEGVRGKIDGKDIYIGKEEM--------------------KQGQT 549
                    +F   PG+G++  ++ K I +G  ++                    + G+T
Sbjct: 746 NSGWLLDTSDFSALPGKGIQCLVNEKMILVGNRKLMSENAINIPDHVEKFVEDLEESGKT 805

Query: 550 LGYVFCEEMAVGSFGLSDSCRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAV 609
              V      VG  G++D  +  A   +E L  +G++  M+TGD    A    +++   +
Sbjct: 806 GVIVAYNGKLVGVMGIADPLKREAALVVEGLLRMGVRPIMVTGDNWRTARAVAKEV--GI 865

Query: 610 DVIHSELLPKEKANVIKEFKRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTG 669
           + + +E++P  KA+VI+  ++D    AMVGDG+ND+PALA AD+G+++G +G+ +A +  
Sbjct: 866 EDVRAEVMPAGKADVIRSLQKDGSTVAMVGDGINDSPALAAADVGMAIG-AGTDVAIEAA 925

Query: 670 NVILMSNDITKIPQAIKLARKSHAKVVQNVILSITTKIAILGLAIAG--HPLV------W 684
           + +LM N++  +  AI L+RK+  ++  N + ++   +  + +A AG   P++      W
Sbjct: 926 DYVLMRNNLEDVITAIDLSRKTLTRIRLNYVFAMAYNVVSIPIA-AGVFFPVLRVQLPPW 985

BLAST of CmoCh05G007420.1 vs. TAIR 10
Match: AT1G63440.1 (heavy metal atpase 5 )

HSP 1 Score: 215.7 bits (548), Expect = 1.4e-55
Identity = 177/594 (29.80%), Postives = 288/594 (48.48%), Query Frame = 0

Query: 146 DYMEAGSIVFLFSI-AEWLESRASHKANAAMWSLMSLAPQKATIAE-------TG-EVVE 205
           D+ E  +++  F I  ++LE  A  K + A+  LM+LAP  A +         TG E ++
Sbjct: 404 DFFETSAMLISFIILGKYLEVMAKGKTSQAIAKLMNLAPDTAILLSLDKEGNVTGEEEID 463

Query: 206 VKEVTLKSVLAVKGGEVIPIDGIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNG 265
            + +    V+ +  G  +  DG V+ G+  V+E  +TGE  PV K K   V  GT+N NG
Sbjct: 464 GRLIQKNDVIKIVPGAKVASDGYVIWGQSHVNESMITGEARPVAKRKGDTVIGGTLNENG 523

Query: 266 YISVQTTVVAEDCVVAKMAEFVEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAA 325
            + V+ T V  +  +A++   VE AQ  K+  Q+  D  +K++ P V+ +S    +   A
Sbjct: 524 VLHVKVTRVGSESALAQIVRLVESAQLAKAPVQKLADRISKFFVPLVIFLS---FSTWLA 583

Query: 326 FRVHNLSHW------------FHLAL----VVLVSACPCALILSTPVAAFCALTKAAMAG 385
           + +    HW            F LAL     V+V ACPCAL L+TP A        A  G
Sbjct: 584 WFLAGKLHWYPESWIPSSMDSFELALQFGISVMVIACPCALGLATPTAVMVGTGVGASQG 643

Query: 386 VLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVIFKLYEMISASTPYFIGNCTKNFSPNSV 445
           VLIKG   LE   K+  + FDKTGT+T G+ V+ K                 KN      
Sbjct: 644 VLIKGGQALERAHKVNCIVFDKTGTLTMGKPVVVKT-------------KLLKNMVLREF 703

Query: 446 YVEIRVSSIESKSSHPMAAALVNHAKLLSTDIK----PEKVEEFENFPGEGVRGKIDGKD 505
           Y    V++ E  S HP+A A+V +AK    D +    PE   +F +  G+GV+  + G++
Sbjct: 704 YE--LVAATEVNSEHPLAKAIVEYAKKFRDDEENPAWPEAC-DFVSITGKGVKATVKGRE 763

Query: 506 IYIGKEEM--------------------KQGQTLGYVFCEEMAVGSFGLSDSCRSGAKEG 565
           I +G + +                       QT   V      +G   +SD  +  A+E 
Sbjct: 764 IMVGNKNLMNDHKVIIPDDAEELLADSEDMAQTGILVSINSELIGVLSVSDPLKPSAREA 823

Query: 566 MEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRDYGATA 625
           +  LKS+ IK+ M+TGD    A    +++   +D + +E  P++KA  +KE +      A
Sbjct: 824 ISILKSMNIKSIMVTGDNWGTANSIAREV--GIDSVIAEAKPEQKAEKVKELQAAGHVVA 883

Query: 626 MVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKVV 682
           MVGDG+ND+PAL  AD+G+++G +G+ +A +  +++LM +++  +  AI L+RK+ +++ 
Sbjct: 884 MVGDGINDSPALVAADVGMAIG-AGTDIAIEAADIVLMKSNLEDVITAIDLSRKTFSRIR 943