CmoCh04G009870.1 (mRNA) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh04G009870.1
Type	mRNA
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Chlorophyll a-b binding proteinic
Location	Cmo_Chr04: 4982967 .. 4983744 (+)
Sequence length	588
RNA-Seq Expression	CmoCh04G009870.1
Synteny	CmoCh04G009870.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTCCAGAAGGTTCTCGAGGGGGCCTTTCCCAGTCACGATGGCCTGGACAAAGAACCCGAACATGGAGAACATGGCCAGCCGCCCGTTCTTGATCTCCTTCACCTTCAGCTCGGCGAAGGTGACGGGGTCGTCGGCCAGTCCGAGTGGGTCGAAGTACTGACCCCCCGGGTACAGGTCGTTGCCTTCGCCCACGCCGTCCAGCCCGTTGATGCGGAACCCTTCAACCAGCCCCATTAGGACCACTTGGAAACCCAAAACGGCTAGGATGCTCTGGGCGTGCACCAGGTTGGGGTTCCCCAAGTAGTCGAGCCCCCCTTCTGAGAAGATCTGGGCTCCGGCCTTGAACCAGACTGGCTCCTTGAAGTCCACTTTCAACCATTTTTCTAGCACCTCTGGTGTTATGCACCCGAAGGCTCCCAACATCGCCCACCTCCCGTGGATTACCTGCAATTCCGTTCGACCATATGTAAATCAAAATTTGAATTCATCAAATTAATACCTTAATTAATTCTTAAGTAAGGATGGGGATGGGAATTACCTCAAGAGCTCTGTTCTTGGCGAAGGCCTCGGGATCGGCGGACAACCCAGCAGTGTCCCATCCATAATCGCCGGGGAACTCTCCGTTCAAGTAGGAGGGAGTCTGAGCGGAAAACGGCCCCAAGTACTTGACTCTGTCTGGTCCATACCACAGTTCATTTCCCTATTCAAAATTGAAAAAAAGAAGAAAACATCATCAATTTCAATACTCCATAAGACAAAAAGGAAGCAAAGATAG

mRNA sequence

ATGGTCCAGAAGGTTCTCGAGGGGGCCTTTCCCAGTCACGATGGCCTGGACAAAGAACCCGAACATGGAGAACATGGCCAGCCGCCCGTTCTTGATCTCCTTCACCTTCAGCTCGGCGAAGGTGACGGGGTCGTCGGCCAGTCCGAGTGGGTCGAAGTACTGACCCCCCGGTCGAGCCCCCCTTCTGAGAAGATCTGGGCTCCGGCCTTGAACCAGACTGGCTCCTTGAAGTCCACTTTCAACCATTTTTCTAGCACCTCTGGTGTTATGCACCCGAAGGCTCCCAACATCGCCCACCTCCCGTGGATTACCTGCAATTCCGTTCGACCATATGATGGGGATGGGAATTACCTCAAGAGCTCTGTTCTTGGCGAAGGCCTCGGGATCGGCGGACAACCCAGCAGTGTCCCATCCATAATCGCCGGGGAACTCTCCGTTCAAGTAGGAGGGAGTCTGAGCGGAAAACGGCCCCAAGTACTTGACTCTGTCTGGTCCATACCACAGTTCATTTCCCTATTCAAAATTGAAAAAAAGAAGAAAACATCATCAATTTCAATACTCCATAAGACAAAAAGGAAGCAAAGATAG

Coding sequence (CDS)

ATGGTCCAGAAGGTTCTCGAGGGGGCCTTTCCCAGTCACGATGGCCTGGACAAAGAACCCGAACATGGAGAACATGGCCAGCCGCCCGTTCTTGATCTCCTTCACCTTCAGCTCGGCGAAGGTGACGGGGTCGTCGGCCAGTCCGAGTGGGTCGAAGTACTGACCCCCCGGTCGAGCCCCCCTTCTGAGAAGATCTGGGCTCCGGCCTTGAACCAGACTGGCTCCTTGAAGTCCACTTTCAACCATTTTTCTAGCACCTCTGGTGTTATGCACCCGAAGGCTCCCAACATCGCCCACCTCCCGTGGATTACCTGCAATTCCGTTCGACCATATGATGGGGATGGGAATTACCTCAAGAGCTCTGTTCTTGGCGAAGGCCTCGGGATCGGCGGACAACCCAGCAGTGTCCCATCCATAATCGCCGGGGAACTCTCCGTTCAAGTAGGAGGGAGTCTGAGCGGAAAACGGCCCCAAGTACTTGACTCTGTCTGGTCCATACCACAGTTCATTTCCCTATTCAAAATTGAAAAAAAGAAGAAAACATCATCAATTTCAATACTCCATAAGACAAAAAGGAAGCAAAGATAG

Protein sequence

MVQKVLEGAFPSHDGLDKEPEHGEHGQPPVLDLLHLQLGEGDGVVGQSEWVEVLTPRSSPPSEKIWAPALNQTGSLKSTFNHFSSTSGVMHPKAPNIAHLPWITCNSVRPYDGDGNYLKSSVLGEGLGIGGQPSSVPSIIAGELSVQVGGSLSGKRPQVLDSVWSIPQFISLFKIEKKKKTSSISILHKTKRKQR

Homology

[Close]

BLAST of CmoCh04G009870.1 vs. ExPASy TrEMBL
Match: C4J6D3 (Uncharacterized protein OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 1.6e-18
Identity = 70/203 (34.48%), Postives = 103/203 (50.74%), Query Frame = 0


Query: 1   MVQKVLEGAFPSHDGLDKEPEHGEHGQPPVLDLLHLQLGEGDGVVGQSEWVEVLTP---- 60
           +V++VLEG     DGLD+E EHGEHG+  VLDLL L+LGEGDGVVG+++ VEVL      
Sbjct: 46  VVEQVLEGPLAGDDGLDEEAEHGEHGEAAVLDLLDLELGEGDGVVGEAQRVEVLAAGVEV 105

Query: 61  -RSSPPSEKIWAPALNQT--------------------------GSLKSTFNHFSSTSGV 120
              +   E + A AL++                            +L+          G+
Sbjct: 106 VALADAVEAVDAEALDEAHEDDLEAEHGQDALRVHQVGVAQVVQPALREDLRAGLEPHGL 165

Query: 121 MHPKA-PNIAHLPWITCNSVRPYDGDGNYLKSSVLGEGLGIGGQPSSVPSIIAGELSVQV 172
           +   A P + HL        +      ++L+  V  E L + GQP  VP+++AGEL+ QV
Sbjct: 166 LEVHAHPLLQHLRGDAAQRTQHRPPPVDHLQRPVPRERLRVRGQPGGVPAVVAGELAGQV 225

[Close]

BLAST of CmoCh04G009870.1 vs. ExPASy TrEMBL
Match: A0A3B5Z6C6 (Uncharacterized protein OS=Triticum aestivum OX=4565 PE=4 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 8.9e-17
Identity = 68/204 (33.33%), Postives = 99/204 (48.53%), Query Frame = 0


Query: 1   MVQKVLEGAFPSHDGLDKEPEHGEHGQPPVLDLLHLQLGEGDGVVGQSEWVEVLTP---- 60
           +V++VL+ A    D LD+EP+HGEHG+  +LDLLHL+L EGD VVG++E VEVL      
Sbjct: 28  VVEQVLQWALAGDDSLDEEPKHGEHGETAILDLLHLELSEGDRVVGKAERVEVLAAGVEV 87

Query: 61  -RSSPPSEKIWAPALNQTGSLKSTFNHFSST--------------------------SGV 120
              +   E I A   +Q         H                               G+
Sbjct: 88  VALADTVETIDAEPFDQAHEKDLEAQHGKDALRVDEVGVPKVVEATLGEDLRAGLEPDGL 147

Query: 121 MHPKA-PNIAHLPWITCNSVRPYDGDGNYLKSSVLGEGLGIGGQPSSVPSIIAGELSVQV 173
           +   A P + HL      S        ++L+  V  E L +GG+P  VP+++A EL+VQV
Sbjct: 148 LELDAHPLLEHLGEDAAKSAEHRPPPVDHLQRPVPSERLRVGGKPGGVPAVVARELAVQV 207

[Close]

BLAST of CmoCh04G009870.1 vs. ExPASy TrEMBL
Match: A0A3L6T734 (Uncharacterized protein OS=Panicum miliaceum OX=4540 GN=C2845_PM03G05550 PE=4 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 7.5e-16
Identity = 68/198 (34.34%), Postives = 102/198 (51.52%), Query Frame = 0


Query: 1   MVQKVLEGAFPSHDGLDKEPEHGEHGQPPVLDLLHLQLGEGDGVVGQSEWVEVL--TPRS 60
           +V +VLE A    DGLD+E EHGEHG+  VL+LL L+LG+G GVVGQ+E VE      R 
Sbjct: 49  VVGEVLERALSGDDGLDEESEHGEHGEAAVLELLDLELGQGLGVVGQAEGVEAAAGVERV 108

Query: 61  SPPSEKIWAPALNQTGSLKSTFN----------HFSSTSGVMHP----------KAPNIA 120
              +E+    A+   G+ +                +  + V+ P          +   +A
Sbjct: 109 DDLAERAAGNAVALDGAHEHDLAGPDGEDALGVDQAGVAEVVEPALAEDLGAGLEPDGLA 168

Query: 121 HLPWITCNSV------RPYDGDGNY--LKSSVLGEGLGIGGQPSSVPSIIAGELSVQVGG 169
            L  +    +      R   G      L+ +VLGEGLG+ GQP  VP+++AGEL+ QV  
Sbjct: 169 ELDAVAGEQLGEDAAERAEHGPAGVDDLQLAVLGEGLGVSGQPRGVPAVVAGELAGQVAR 228

[Close]

BLAST of CmoCh04G009870.1 vs. ExPASy TrEMBL
Match: A0A2P2LQR3 (Chlorophyll a-b binding proteinic OS=Rhizophora mucronata OX=61149 PE=4 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 9.9e-16
Identity = 61/204 (29.90%), Postives = 98/204 (48.04%), Query Frame = 0


Query: 1   MVQKVLEGAFPSHDGLDKEPEHGEHGQPPVLDLLHLQLGEGDGVVGQSEWVEVLTPRSSP 60
           +V ++L+ +  SH+GL+KE EH EHGQ  +LDLLHLQL +   V+ QS+WV+  T     
Sbjct: 69  VVSQILQWSLTSHNGLNKESEHREHGQSAILDLLHLQLSKSLWVISQSQWVKAAT----- 128

Query: 61  PSEKIWAPALNQTGSLKSTFNHFSSTSGVMHP---------------------------- 120
                W   L++  +  +   H S    +  P                            
Sbjct: 129 --RVEWVCDLSEGPTRNTVTLHSSHQDNLTSPDGQDALSMDQAGVAQVVKATLAEDLGSG 188

Query: 121 -KAPNIAHLPWITCNSVRPYDGDG--------NYLKSSVLGEGLGIGGQPSSVPSIIAGE 168
            +  ++  L  IT   +R    +G        ++L+  + G+GL +  +PS VP+++ GE
Sbjct: 189 LEPDSLTELDSITSQQLREDTAEGSKHGPSAVDHLELPIPGKGLWVSREPSGVPAVVTGE 248

[Close]

BLAST of CmoCh04G009870.1 vs. ExPASy TrEMBL
Match: T1N1P0 (Uncharacterized protein OS=Triticum urartu OX=4572 PE=4 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 1.3e-15
Identity = 66/204 (32.35%), Postives = 99/204 (48.53%), Query Frame = 0


Query: 1   MVQKVLEGAFPSHDGLDKEPEHGEHGQPPVLDLLHLQLGEGDGVVGQSEWVEVLTP---- 60
           +V++VL+ A    D LD+EP+H EHG+  + DLLHL+L EGD VVG++E VEVL      
Sbjct: 21  VVEQVLQWALTGDDSLDEEPKHREHGETAIFDLLHLKLSEGDRVVGKAERVEVLAAGVEV 80

Query: 61  -RSSPPSEKIWAPALNQTGSLKSTFNHFSST--------------------------SGV 120
              +   E + A   N+T        H                               G+
Sbjct: 81  VALADAIETVDAEPFNETHEKDLEAEHGKDALRVDEVGVPKVIEATLGEDLRAGLEPDGL 140

Query: 121 MHPKA-PNIAHLPWITCNSVRPYDGDGNYLKSSVLGEGLGIGGQPSSVPSIIAGELSVQV 173
           +   A P + HL      S +      ++L+  V  E L +G +P  VP+++AGEL+VQV
Sbjct: 141 LELDAHPLLEHLGEDAPKSAKHRPPPVDHLQRPVPRERLRVGRKPGGVPAVVAGELAVQV 200

[Close]

BLAST of CmoCh04G009870.1 vs. NCBI nr
Match: KAG6421321.1 (hypothetical protein SASPL_117872 [Salvia splendens])

HSP 1 Score: 116.7 bits (291), Expect = 2.2e-22
Identity = 75/165 (45.45%), Postives = 92/165 (55.76%), Query Frame = 0


Query: 1    MVQKVLEGAFPSHDGLDKEPEHGEHGQPPVLDLLHLQLGEGDGVVGQSEWVEVLTPRSSP 60
            M+QKVLE +    D L +E EHGEHGQP VLDLL LQLG+GD VVGQ+EWVEVL  R   
Sbjct: 1132 MIQKVLERSLSGDDSLHEETEHGEHGQPSVLDLLDLQLGKGDWVVGQAEWVEVLAAR--- 1191

Query: 61   PSEKIWAPALNQTGSLKSTFNHFSSTSGVMHPKAPNIAHLPWITCNSVRPYDGDGNYLKS 120
                +   AL+       +     S    + PK            NS      D   ++ 
Sbjct: 1192 ----VEVVALSDPVKAVDSEPLHESHEYNLEPK------------NSQNALSMD--QIRR 1251

Query: 121  SVLGEGLGIGGQPSSVPSIIAGELSVQVGGSLSGKRPQVLDSVWS 166
             VLG+GLGIGGQ   VPS++ GE SV+VG SLS +  QVLD+V S
Sbjct: 1252 PVLGKGLGIGGQTGRVPSVVTGEFSVEVGRSLSREGSQVLDTVRS 1275

[Close]

BLAST of CmoCh04G009870.1 vs. NCBI nr
Match: ACR36733.1 (unknown [Zea mays])

HSP 1 Score: 102.8 bits (255), Expect = 3.4e-18
Identity = 70/203 (34.48%), Postives = 103/203 (50.74%), Query Frame = 0


Query: 1   MVQKVLEGAFPSHDGLDKEPEHGEHGQPPVLDLLHLQLGEGDGVVGQSEWVEVLTP---- 60
           +V++VLEG     DGLD+E EHGEHG+  VLDLL L+LGEGDGVVG+++ VEVL      
Sbjct: 46  VVEQVLEGPLAGDDGLDEEAEHGEHGEAAVLDLLDLELGEGDGVVGEAQRVEVLAAGVEV 105

Query: 61  -RSSPPSEKIWAPALNQT--------------------------GSLKSTFNHFSSTSGV 120
              +   E + A AL++                            +L+          G+
Sbjct: 106 VALADAVEAVDAEALDEAHEDDLEAEHGQDALRVHQVGVAQVVQPALREDLRAGLEPHGL 165

Query: 121 MHPKA-PNIAHLPWITCNSVRPYDGDGNYLKSSVLGEGLGIGGQPSSVPSIIAGELSVQV 172
           +   A P + HL        +      ++L+  V  E L + GQP  VP+++AGEL+ QV
Sbjct: 166 LEVHAHPLLQHLRGDAAQRTQHRPPPVDHLQRPVPRERLRVRGQPGGVPAVVAGELAGQV 225

[Close]

BLAST of CmoCh04G009870.1 vs. NCBI nr
Match: KAF6992250.1 (hypothetical protein CFC21_009263 [Triticum aestivum])

HSP 1 Score: 97.1 bits (240), Expect = 1.8e-16
Identity = 68/204 (33.33%), Postives = 99/204 (48.53%), Query Frame = 0


Query: 1   MVQKVLEGAFPSHDGLDKEPEHGEHGQPPVLDLLHLQLGEGDGVVGQSEWVEVLTP---- 60
           +V++VL+ A    D LD+EP+HGEHG+  +LDLLHL+L EGD VVG++E VEVL      
Sbjct: 28  VVEQVLQWALAGDDSLDEEPKHGEHGETAILDLLHLELSEGDRVVGKAERVEVLAAGVEV 87

Query: 61  -RSSPPSEKIWAPALNQTGSLKSTFNHFSST--------------------------SGV 120
              +   E I A   +Q         H                               G+
Sbjct: 88  VALADTVETIDAEPFDQAHEKDLEAQHGKDALRVDEVGVPKVVEATLGEDLRAGLEPDGL 147

Query: 121 MHPKA-PNIAHLPWITCNSVRPYDGDGNYLKSSVLGEGLGIGGQPSSVPSIIAGELSVQV 173
           +   A P + HL      S        ++L+  V  E L +GG+P  VP+++A EL+VQV
Sbjct: 148 LELDAHPLLEHLGEDAAKSAEHRPPPVDHLQRPVPSERLRVGGKPGGVPAVVARELAVQV 207

[Close]

BLAST of CmoCh04G009870.1 vs. NCBI nr
Match: RLN33992.1 (hypothetical protein C2845_PM03G05550 [Panicum miliaceum])

HSP 1 Score: 94.0 bits (232), Expect = 1.6e-15
Identity = 68/198 (34.34%), Postives = 102/198 (51.52%), Query Frame = 0


Query: 1   MVQKVLEGAFPSHDGLDKEPEHGEHGQPPVLDLLHLQLGEGDGVVGQSEWVEVL--TPRS 60
           +V +VLE A    DGLD+E EHGEHG+  VL+LL L+LG+G GVVGQ+E VE      R 
Sbjct: 49  VVGEVLERALSGDDGLDEESEHGEHGEAAVLELLDLELGQGLGVVGQAEGVEAAAGVERV 108

Query: 61  SPPSEKIWAPALNQTGSLKSTFN----------HFSSTSGVMHP----------KAPNIA 120
              +E+    A+   G+ +                +  + V+ P          +   +A
Sbjct: 109 DDLAERAAGNAVALDGAHEHDLAGPDGEDALGVDQAGVAEVVEPALAEDLGAGLEPDGLA 168

Query: 121 HLPWITCNSV------RPYDGDGNY--LKSSVLGEGLGIGGQPSSVPSIIAGELSVQVGG 169
            L  +    +      R   G      L+ +VLGEGLG+ GQP  VP+++AGEL+ QV  
Sbjct: 169 ELDAVAGEQLGEDAAERAEHGPAGVDDLQLAVLGEGLGVSGQPRGVPAVVAGELAGQVAR 228

[Close]

BLAST of CmoCh04G009870.1 vs. NCBI nr
Match: VVW58041.1 (unnamed protein product, partial [Nymphaea colorata])

HSP 1 Score: 92.4 bits (228), Expect = 4.5e-15
Identity = 63/197 (31.98%), Postives = 101/197 (51.27%), Query Frame = 0


Query: 1   MVQKVLEGAFPSHDGLDKEPEHGEHGQPPVLDLLHLQLGEGDGVVGQSEWVEVLT----- 60
           +V +VL+G    H+GL KEPEHGEHG+  VLDLLHLQ  +  GV+GQ++W+E  T     
Sbjct: 11  VVSQVLQGTLAGHNGLHKEPEHGEHGKSAVLDLLHLQFCKCLGVIGQTQWIEATTWVQWV 70

Query: 61  ------------PRSSPPSEKIWAP----ALNQTGSLKSTFNHFSSTSGVMHPKAP-NIA 120
                       P +      + +P    AL+   +  +     S T  +     P ++A
Sbjct: 71  GHLTEWSASNAIPLNCTHENDLRSPDGQDALSMDQARVAQVVKSSLTEDLSTGLEPDSLA 130

Query: 121 HLPWITCNSVR-------PYDGDG-NYLKSSVLGEGLGIGGQPSSVPSIIAGELSVQVGG 168
            L  +    +R        +   G ++L+  VLG+GL +  + S VP+I+  E + +VG 
Sbjct: 131 ELDSVPSEQLREDAPKCTQHSPPGMDHLELPVLGKGLRVSRETSGVPAIVTREFTGEVGR 190

The following BLAST results are available for this feature:

BLAST of CmoCh04G009870.1 vs.


Match Name	E-value	Identity	Description

BLAST of CmoCh04G009870.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
C4J6D3	1.6e-18	34.48	Uncharacterized protein OS=Zea mays OX=4577 PE=2 SV=1	[more]
A0A3B5Z6C6	8.9e-17	33.33	Uncharacterized protein OS=Triticum aestivum OX=4565 PE=4 SV=1	[more]
A0A3L6T734	7.5e-16	34.34	Uncharacterized protein OS=Panicum miliaceum OX=4540 GN=C2845_PM03G05550 PE=4 SV...	[more]
A0A2P2LQR3	9.9e-16	29.90	Chlorophyll a-b binding proteinic OS=Rhizophora mucronata OX=61149 PE=4 SV=1	[more]
T1N1P0	1.3e-15	32.35	Uncharacterized protein OS=Triticum urartu OX=4572 PE=4 SV=1	[more]

BLAST of CmoCh04G009870.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6421321.1	2.2e-22	45.45	hypothetical protein SASPL_117872 [Salvia splendens]	[more]
ACR36733.1	3.4e-18	34.48	unknown [Zea mays]	[more]
KAF6992250.1	1.8e-16	33.33	hypothetical protein CFC21_009263 [Triticum aestivum]	[more]
RLN33992.1	1.6e-15	34.34	hypothetical protein C2845_PM03G05550 [Panicum miliaceum]	[more]
VVW58041.1	4.5e-15	31.98	unnamed protein product, partial [Nymphaea colorata]	[more]

BLAST of CmoCh04G009870.1 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..29
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 11..28

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmoCh04G009870	CmoCh04G009870	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh04G009870.1:exon:5058	CmoCh04G009870.1:exon:5058	exon
CmoCh04G009870.1:exon:5059	CmoCh04G009870.1:exon:5059	exon
CmoCh04G009870.1:exon:5060	CmoCh04G009870.1:exon:5060	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh04G009870.1:cds	CmoCh04G009870.1:cds	CDS
CmoCh04G009870.1:cds	CmoCh04G009870.1:cds_2	CDS
CmoCh04G009870.1:cds	CmoCh04G009870.1:cds_3	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmoCh04G009870.1	CmoCh04G009870.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009987	cellular process
biological_process	GO:0043170	macromolecule metabolic process
biological_process	GO:0044238	primary metabolic process
cellular_component	GO:0043229	intracellular organelle
cellular_component	GO:0016020	membrane
cellular_component	GO:0032991	protein-containing complex
molecular_function	GO:0043167	ion binding
molecular_function	GO:0016829	lyase activity
molecular_function	GO:0000166	nucleotide binding
molecular_function	GO:0005515	protein binding