CmoCh03G011560.1 (mRNA) Cucurbita moschata (Rifu) v1

BLAST of CmoCh03G011560.1 vs. ExPASy Swiss-Prot
Match: Q9LZS7 (GDSL esterase/lipase At5g03610 OS=Arabidopsis thaliana OX=3702 GN=At5g03610 PE=2 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 5.3e-97
Identity = 182/354 (51.41%), Postives = 243/354 (68.64%), Query Frame = 0

Query: 1   MDFVLRLLFSLLLLI--SLFSGRVSAGGVYNHHHHHQRHRLRPTKLFVFGDSYVDTGNIL 60
           MD +++L F L + +  SL  G ++  GV   + +H  +  RPTKLFVFGDSY DTGNI 
Sbjct: 1   MDSLIKLFFCLFIFLCTSLLFGEIN--GVEGSNQNHHLYPFRPTKLFVFGDSYADTGNIK 60

Query: 61  IPLSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYVGLKSPIPFTVQNRVGLRRFKETG 120
              SS+ +FPYGITFPGKP+GRFSDGRV TDF A++VG+KSPIP+  ++  G +R  + G
Sbjct: 61  KAFSSSWKFPYGITFPGKPAGRFSDGRVATDFLAKFVGIKSPIPYFWKDYAGKKRL-QYG 120

Query: 121 VNFAFGGTGVFDTSFPFPNMTTQIDFFQQLQHGESLSTKTDNQFSAALVSVSGNDYSLDI 180
           +NFA+GGTGVF+T  P PNMTTQID FQ +     +    +   S ALVSV+GNDYS  I
Sbjct: 121 MNFAYGGTGVFNTQTPLPNMTTQIDIFQNILTTGDIYYPPELTSSVALVSVAGNDYSNFI 180

Query: 181 ITNGPKQGLKPFINTVVNQIIVDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNE 240
             N P      FI  VV+Q  V+L+RI  LGVKKIAV  L PLGCLP FT  +SF++CNE
Sbjct: 181 ALNRPASEFPAFIKQVVDQTEVNLRRIHALGVKKIAVPSLQPLGCLPPFTFVTSFQRCNE 240

Query: 241 TINSFVKFHNFLLKKAVKKLNQETKHSSKVFVLDMYGAFMSIIEGEKRYNNGV--FKTPL 300
           T N+ V  HN LL++ V KLN ETK S+ + +LD+Y AF+++ +  K  N G   F++PL
Sbjct: 241 TQNALVNLHNNLLQQVVAKLNNETKQSTFI-ILDLYNAFLTVFK-NKGSNPGSTRFESPL 300

Query: 301 KPCCFGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAFSALK 351
           KPCC GV   ++CGSVD +G K +++C +PK AF+WD +HPT++GW + +S L+
Sbjct: 301 KPCCVGVSREYNCGSVDEKGVKKYIVCDNPKTAFFWDGLHPTEEGWRSVYSVLR 349

BLAST of CmoCh03G011560.1 vs. ExPASy Swiss-Prot
Match: Q9SF94 (GDSL esterase/lipase At3g09930 OS=Arabidopsis thaliana OX=3702 GN=At3g09930 PE=2 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 9.6e-91
Identity = 173/343 (50.44%), Postives = 230/343 (67.06%), Query Frame = 0

Query: 13  LLISLFSGRVSA---GGVYNHHHHHQRHRLRPTKLFVFGDSYVDTGNILIPLSSAQQFPY 72
           LLISLF    S+   G   ++  H    RLRP +LFVFGDSY DTGNI   LS + + PY
Sbjct: 6   LLISLFLFSFSSFFLGAESDYPQHSNLKRLRPNRLFVFGDSYADTGNIRKSLSDSWKIPY 65

Query: 73  GITFPGKPSGRFSDGRVLTDFAARYVGLKSPIPFTVQNRVGLRRFKETGVNFAFGGTGVF 132
           GITFP KPSGRFSDGRV TDF ARY+G+KSPIP+T ++  G  R    G+N+A+GGTGVF
Sbjct: 66  GITFPQKPSGRFSDGRVATDFLARYLGIKSPIPYTWKDYAGKERLL-YGMNYAYGGTGVF 125

Query: 133 DT-SFPFPNMTTQIDFFQQLQHGESLSTKTDNQFSAALVSVSGNDYSLDIITNGPKQGLK 192
            T   P PNMTTQID+FQ++    ++ + +D   S ALVSV+GNDY+  +    P   L 
Sbjct: 126 KTKDNPLPNMTTQIDYFQRVLAAGNIYSPSDLPSSLALVSVAGNDYATFLALKRPLTELP 185

Query: 193 PFINTVVNQIIVDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNETINSFVKFHN 252
            F+  VV+QI V+  RI +LGV KI +  + PLGCLP+ T  +SF++CN T N+    HN
Sbjct: 186 AFMKQVVDQIAVNAMRIHKLGVNKIVIPSMQPLGCLPSITVFNSFQRCNATDNASTNLHN 245

Query: 253 FLLKKAVKKLNQETKHSSKVFVLDMYGAFMSIIEGEKRYNNGV--FKTPLKPCCFGVKSG 312
           +LL KA+ +LN ETK S+ V VLD Y AF+++ +  K    GV  F  PLKPCC GV S 
Sbjct: 246 YLLHKAIARLNNETKPSTFV-VLDHYNAFLTVFK-NKGPEPGVSRFGNPLKPCCVGVNSS 305

Query: 313 FDCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAFSAL 350
           +DC +VD +G+K +++C+DPK AF+WD  HP+++GW + +S L
Sbjct: 306 YDCSNVDEKGEKKYIICEDPKAAFFWDIFHPSEEGWRSVYSVL 345

BLAST of CmoCh03G011560.1 vs. ExPASy Swiss-Prot
Match: Q9LZS8 (GDSL esterase/lipase At5g03600 OS=Arabidopsis thaliana OX=3702 GN=At5g03600 PE=3 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 8.8e-68
Identity = 147/323 (45.51%), Postives = 198/323 (61.30%), Query Frame = 0

Query: 39  LRP---TKLFVFGDSYVDTGNILIPLSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYV 98
           LRP    KLFVFGDSY DTGN     + A   PYGITFPGKPSGR+ DG + TDF  + +
Sbjct: 7   LRPRKVPKLFVFGDSYADTGNTKRD-TEAWAIPYGITFPGKPSGRYCDGLIATDFLEKVL 66

Query: 99  GLKSPIPFTVQNR-VGLRRFKETGVNFAFGGTGVFDTS--FPFPNMTTQIDFFQQL-QHG 158
           G +SP  +    R  GL+R    G+NFAFGG+ + D+S   PFPN+T Q++F   L   G
Sbjct: 67  GAESPYLYRTHGRDKGLKR----GMNFAFGGSKMLDSSPNSPFPNITAQVNFLVDLVLAG 126

Query: 159 ESLSTKTDNQFSAALVSVSGNDYSLDIITNGPKQGLKPFINTVVNQIIVDLKRIGELGVK 218
                 T +  S  L+S +G DY   I  N P  GLK  +  VV+ + V++  +G L  K
Sbjct: 127 RVYGDITPSDVS--LISYAGGDYIYYIDQNRPAAGLKALVEKVVDNLRVNMIVLGGLLFK 186

Query: 219 KIAVTGLGPLGCLPAFTAPSSFKKCNETINSFVKFHNFLLKKAVKKLNQET---KHSSKV 278
           KIAVT L P+GCLP++T+ SSFK CNE+ ++ V+ HN LLKK V KLN+++   K     
Sbjct: 187 KIAVTSLQPIGCLPSYTSASSFKSCNESQSALVELHNKLLKKVVAKLNEQSRVMKKEQHF 246

Query: 279 FVLDMYGAFMSII--EGEKRYNNGVFKTPLKPCCFGVKSGFDCGSVDGEGKKMFVLCKDP 338
           F++D++ AFM+++  +G KR     FK P+K CC G      CG    +G K++ LC DP
Sbjct: 247 FIIDIHNAFMTVMKNKGSKR-----FKNPMKSCCEGY-----CGR-SSDGGKLYTLCDDP 306

Query: 339 KKAFYWDAVHPTQQGWAAAFSAL 350
           K  F+WDAVHPTQ+GW + +S L
Sbjct: 307 KSFFFWDAVHPTQEGWRSIYSVL 311

BLAST of CmoCh03G011560.1 vs. ExPASy Swiss-Prot
Match: Q9LZS9 (GDSL esterase/lipase At5g03590 OS=Arabidopsis thaliana OX=3702 GN=At5g03590 PE=3 SV=2)

HSP 1 Score: 251.5 bits (641), Expect = 1.4e-65
Identity = 153/356 (42.98%), Postives = 204/356 (57.30%), Query Frame = 0

Query: 1   MDFVLRLLFSLLLLISLFSGRVSAGGVYNHHHHHQRHRLRPTKLFVFGDSYVDTGNILIP 60
           M ++++L FSL    SLF G   A G      +      +  KLFVFG+SY DTGN + P
Sbjct: 1   MHYLMKLFFSL----SLFFGINGAVG-----SNQPSQANKNPKLFVFGNSYADTGN-MKP 60

Query: 61  LSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYVGLKSPIPFTV--QNRVGLRRFKETG 120
            + + + PYGITFPGKPSGR+SDG   TDF A+ +G K P  +    + +V L R    G
Sbjct: 61  TALSWKLPYGITFPGKPSGRYSDGLTATDFLAKQLGAKLPYLWRTHGKKKVKLNR----G 120

Query: 121 VNFAFGGTGVFDTSFP-FPNMTTQIDFFQQLQHGESLST-KTDNQFSAALVSVSGNDYSL 180
           +NFAFGG+ VFD+     PN++TQ+ F   L     + T   D   S AL+S SG DY  
Sbjct: 121 MNFAFGGSEVFDSPVDRSPNISTQVGFLVNLALARRVYTIDGDLASSYALLSYSGTDYYG 180

Query: 181 DIITNGPKQGLKPFINTVVNQIIVDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKC 240
            I  N        F+  +V  I   L  +  L  K IAVT L PLGCLP  T  SSF+ C
Sbjct: 181 FIDQNPNMAAYPAFVEFIVEDIQYSLGIMNGLKFKNIAVTSLHPLGCLPRVTVASSFRSC 240

Query: 241 NETINSFVKFHNFLLKKAVKKLNQETKHSSK---VFVLDMYGAFMSIIEGEKRYNNGVFK 300
           NE+ +  V+ HN  LKKAV KLN+E K  +K     ++D++ AFM+I+E   +  N  FK
Sbjct: 241 NESYSDLVRLHNESLKKAVAKLNKEDKFRTKGDRFVIVDLHKAFMTILE---KKGNKRFK 300

Query: 301 TPLKPCCFGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAFSAL 350
           +PLKPCC G     DC  +D +G K + LC DPK AF+WD ++PTQ+GW + +S L
Sbjct: 301 SPLKPCCEG-----DCARMDMKGAKKYTLCNDPKSAFFWDEINPTQEGWRSIYSLL 334

BLAST of CmoCh03G011560.1 vs. ExPASy Swiss-Prot
Match: Q8RWJ4 (GDSL esterase/lipase At2g36325 OS=Arabidopsis thaliana OX=3702 GN=At2g36325 PE=2 SV=2)

HSP 1 Score: 243.4 bits (620), Expect = 3.8e-63
Identity = 134/313 (42.81%), Postives = 183/313 (58.47%), Query Frame = 0

Query: 40  RPTKLFVFGDSYVDTGNILIPLSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYVGLKS 99
           +PTKLFVFGDSY DTGN    +  + +FP GITFPG P+GRFSDGRV TD+ A+Y+G+++
Sbjct: 44  KPTKLFVFGDSYADTGNTPFLIVPSWRFPNGITFPGIPTGRFSDGRVSTDYLAKYIGVRT 103

Query: 100 PIPFTVQNRVGLRRFKETGVNFAFGGTGVFDTSFPF-PNMTTQIDFFQQLQHGESLSTKT 159
           PI +        R   + G+NFA+GG G F+T F   P  + QID F+QL    ++ +  
Sbjct: 104 PITYKWGKYGRPRLAVKRGMNFAYGGAGAFETMFKLVPTASVQIDSFEQLLM-RNVYSPA 163

Query: 160 DNQFSAALVSVSGNDYSLDIITNGPKQGLKPFINTVVNQIIVDLKRIGELGVKKIAVTGL 219
           D   S A  S+ GNDY      NG ++G       VV QI++D+KRI +LGV+K+ V   
Sbjct: 164 DLNSSVAFFSIIGNDYLTYDRRNGSEEGRSALTRKVVKQILLDVKRIKDLGVRKVLVALS 223

Query: 220 GPLGCLPAFTAPSSFKKCNETINSFVKFHNFLLKKAVKKLN-QETKHSSKVFV-LDMYGA 279
            P  CLP    P   K C +T ++    HN LL+K + KLN +E  ++ K F+ LD+Y A
Sbjct: 224 PPQKCLPKLVTP---KGC-DTNDTSTYLHNSLLRKGLIKLNDKEINNNDKSFMTLDLYNA 283

Query: 280 FMSIIEGEKRYNNGVFKTPLKPCCFGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWDAVH 339
           F++I + +       F  P K CC   K G  CG     GKK++ LC DPK  F+WD VH
Sbjct: 284 FVTIFKNKGVSGVSTFPDPFKACC-ATKRGTFCGDRSLSGKKLYTLCDDPKSFFFWDNVH 343

Query: 340 PTQQGWAAAFSAL 350
            + QGW + FS L
Sbjct: 344 ISDQGWRSVFSLL 350

BLAST of CmoCh03G011560.1 vs. ExPASy TrEMBL
Match: A0A6J1EC94 (GDSL esterase/lipase At5g03610-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111433004 PE=3 SV=1)

HSP 1 Score: 732.6 bits (1890), Expect = 7.6e-208
Identity = 356/356 (100.00%), Postives = 356/356 (100.00%), Query Frame = 0

Query: 1   MDFVLRLLFSLLLLISLFSGRVSAGGVYNHHHHHQRHRLRPTKLFVFGDSYVDTGNILIP 60
           MDFVLRLLFSLLLLISLFSGRVSAGGVYNHHHHHQRHRLRPTKLFVFGDSYVDTGNILIP
Sbjct: 1   MDFVLRLLFSLLLLISLFSGRVSAGGVYNHHHHHQRHRLRPTKLFVFGDSYVDTGNILIP 60

Query: 61  LSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYVGLKSPIPFTVQNRVGLRRFKETGVN 120
           LSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYVGLKSPIPFTVQNRVGLRRFKETGVN
Sbjct: 61  LSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYVGLKSPIPFTVQNRVGLRRFKETGVN 120

Query: 121 FAFGGTGVFDTSFPFPNMTTQIDFFQQLQHGESLSTKTDNQFSAALVSVSGNDYSLDIIT 180
           FAFGGTGVFDTSFPFPNMTTQIDFFQQLQHGESLSTKTDNQFSAALVSVSGNDYSLDIIT
Sbjct: 121 FAFGGTGVFDTSFPFPNMTTQIDFFQQLQHGESLSTKTDNQFSAALVSVSGNDYSLDIIT 180

Query: 181 NGPKQGLKPFINTVVNQIIVDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNETI 240
           NGPKQGLKPFINTVVNQIIVDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNETI
Sbjct: 181 NGPKQGLKPFINTVVNQIIVDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNETI 240

Query: 241 NSFVKFHNFLLKKAVKKLNQETKHSSKVFVLDMYGAFMSIIEGEKRYNNGVFKTPLKPCC 300
           NSFVKFHNFLLKKAVKKLNQETKHSSKVFVLDMYGAFMSIIEGEKRYNNGVFKTPLKPCC
Sbjct: 241 NSFVKFHNFLLKKAVKKLNQETKHSSKVFVLDMYGAFMSIIEGEKRYNNGVFKTPLKPCC 300

Query: 301 FGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAFSALKAFRNHF 357
           FGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAFSALKAFRNHF
Sbjct: 301 FGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAFSALKAFRNHF 356

BLAST of CmoCh03G011560.1 vs. ExPASy TrEMBL
Match: A0A6J1IKX2 (GDSL esterase/lipase At5g03610-like OS=Cucurbita maxima OX=3661 GN=LOC111478379 PE=3 SV=1)

HSP 1 Score: 698.4 bits (1801), Expect = 1.6e-197
Identity = 341/356 (95.79%), Postives = 347/356 (97.47%), Query Frame = 0

Query: 1   MDFVLRLLFSLLLLISLFSGRVSAGGVYNHHHHHQRHRLRPTKLFVFGDSYVDTGNILIP 60
           MDFVLRLLFSL LLISLFSGR SAGGVYN HHHHQRHRLRPTKLFVFGDSYVDTGNILIP
Sbjct: 1   MDFVLRLLFSLFLLISLFSGRGSAGGVYN-HHHHQRHRLRPTKLFVFGDSYVDTGNILIP 60

Query: 61  LSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYVGLKSPIPFTVQNRVGLRRFKETGVN 120
           LSSAQ FPYGITFP KPSGRFSDGRVLTDFAARYVGLKSPIPF+++NRVGLRR KETGVN
Sbjct: 61  LSSAQSFPYGITFPRKPSGRFSDGRVLTDFAARYVGLKSPIPFSIRNRVGLRRLKETGVN 120

Query: 121 FAFGGTGVFDTSFPFPNMTTQIDFFQQLQHGESLSTKTDNQFSAALVSVSGNDYSLDIIT 180
           FAFGGTGVFDTSFPFPNMTTQIDFFQQLQHG SLSTKTDNQFSAALVSVSGNDYSLDIIT
Sbjct: 121 FAFGGTGVFDTSFPFPNMTTQIDFFQQLQHGHSLSTKTDNQFSAALVSVSGNDYSLDIIT 180

Query: 181 NGPKQGLKPFINTVVNQIIVDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNETI 240
           NGPKQGLKPFINTVVNQIIVDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNE+I
Sbjct: 181 NGPKQGLKPFINTVVNQIIVDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNESI 240

Query: 241 NSFVKFHNFLLKKAVKKLNQETKHSSKVFVLDMYGAFMSIIEGEKRYNNGVFKTPLKPCC 300
           NSFVKFHNFLLKKAVKKLNQETKHSSKVFVLD+YGAFMSIIEG KRYNNG FKTPLKPCC
Sbjct: 241 NSFVKFHNFLLKKAVKKLNQETKHSSKVFVLDVYGAFMSIIEGGKRYNNGEFKTPLKPCC 300

Query: 301 FGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAFSALKAFRNHF 357
           FGVKSGF+CGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAFSALKAFRNHF
Sbjct: 301 FGVKSGFNCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAFSALKAFRNHF 355

BLAST of CmoCh03G011560.1 vs. ExPASy TrEMBL
Match: A0A6J1ECT1 (GDSL esterase/lipase At5g03610-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111433004 PE=3 SV=1)

HSP 1 Score: 613.2 bits (1580), Expect = 6.8e-172
Identity = 296/359 (82.45%), Postives = 320/359 (89.14%), Query Frame = 0

Query: 1   MDFVLRLLFSLLLLISLFSGRVSAGGVYNHHHH---HQRHRLRPTKLFVFGDSYVDTGNI 60
           MDFVLRLLFSLLLLISLFSGR SAGGVYNHHHH   H RHR RPTKLFVFGDSY DTGNI
Sbjct: 1   MDFVLRLLFSLLLLISLFSGRGSAGGVYNHHHHRRRHLRHRFRPTKLFVFGDSYADTGNI 60

Query: 61  LIPLSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYVGLKSPIPFTVQNRVGLRRFKET 120
           L P+S A QFPYGITFPGKP GRFSDGRVLTDFAAR+VGLKSPIPF+V+NRVGLRRFKET
Sbjct: 61  LFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKET 120

Query: 121 GVNFAFGGTGVFDTSFPFPNMTTQIDFFQQLQHGESLSTKTDNQFSAALVSVSGNDYSLD 180
           GVNFAFGGTGVFDT +P PNMTTQIDFFQQL+HG+S+ T TD Q S ALVS SGNDY+  
Sbjct: 121 GVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSIFTNTDAQSSVALVSASGNDYAFY 180

Query: 181 IITNGPKQGLKPFINTVVNQIIVDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCN 240
              N  +QGLKPFI +VV+QIIV+LKRIG LGVKKIAVTGLGPLGCLP FTAPSS+K+CN
Sbjct: 181 FAMNSSRQGLKPFIMSVVSQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKQCN 240

Query: 241 ETINSFVKFHNFLLKKAVKKLNQETKHSSKVFVLDMYGAFMSIIEGEKRYNNGVFKTPLK 300
           ETINS V+FHN LLK+AVKKLNQETKHSSKVFV+D+YGAFMSIIEG KRYNNG FKTPLK
Sbjct: 241 ETINSLVQFHNLLLKEAVKKLNQETKHSSKVFVVDVYGAFMSIIEGRKRYNNGEFKTPLK 300

Query: 301 PCCFGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAFSALKAFRNHF 357
           PCC+GV SGF CGSVDGEGKKMFVLC+D K AF+WD+VHPTQQGWAAA SA K+FRNHF
Sbjct: 301 PCCYGVGSGFSCGSVDGEGKKMFVLCEDQKAAFFWDSVHPTQQGWAAAISAFKSFRNHF 359

BLAST of CmoCh03G011560.1 vs. ExPASy TrEMBL
Match: A0A6J1EIS2 (GDSL esterase/lipase At5g03610-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111433004 PE=3 SV=1)

HSP 1 Score: 613.2 bits (1580), Expect = 6.8e-172
Identity = 296/359 (82.45%), Postives = 320/359 (89.14%), Query Frame = 0

Query: 1   MDFVLRLLFSLLLLISLFSGRVSAGGVYNHHHH---HQRHRLRPTKLFVFGDSYVDTGNI 60
           MDFVLRLLFSLLLLISLFSGR SAGGVYNHHHH   H RHR RPTKLFVFGDSY DTGNI
Sbjct: 2   MDFVLRLLFSLLLLISLFSGRGSAGGVYNHHHHRRRHLRHRFRPTKLFVFGDSYADTGNI 61

Query: 61  LIPLSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYVGLKSPIPFTVQNRVGLRRFKET 120
           L P+S A QFPYGITFPGKP GRFSDGRVLTDFAAR+VGLKSPIPF+V+NRVGLRRFKET
Sbjct: 62  LFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKET 121

Query: 121 GVNFAFGGTGVFDTSFPFPNMTTQIDFFQQLQHGESLSTKTDNQFSAALVSVSGNDYSLD 180
           GVNFAFGGTGVFDT +P PNMTTQIDFFQQL+HG+S+ T TD Q S ALVS SGNDY+  
Sbjct: 122 GVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSIFTNTDAQSSVALVSASGNDYAFY 181

Query: 181 IITNGPKQGLKPFINTVVNQIIVDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCN 240
              N  +QGLKPFI +VV+QIIV+LKRIG LGVKKIAVTGLGPLGCLP FTAPSS+K+CN
Sbjct: 182 FAMNSSRQGLKPFIMSVVSQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKQCN 241

Query: 241 ETINSFVKFHNFLLKKAVKKLNQETKHSSKVFVLDMYGAFMSIIEGEKRYNNGVFKTPLK 300
           ETINS V+FHN LLK+AVKKLNQETKHSSKVFV+D+YGAFMSIIEG KRYNNG FKTPLK
Sbjct: 242 ETINSLVQFHNLLLKEAVKKLNQETKHSSKVFVVDVYGAFMSIIEGRKRYNNGEFKTPLK 301

Query: 301 PCCFGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAFSALKAFRNHF 357
           PCC+GV SGF CGSVDGEGKKMFVLC+D K AF+WD+VHPTQQGWAAA SA K+FRNHF
Sbjct: 302 PCCYGVGSGFSCGSVDGEGKKMFVLCEDQKAAFFWDSVHPTQQGWAAAISAFKSFRNHF 360

BLAST of CmoCh03G011560.1 vs. ExPASy TrEMBL
Match: A0A6J1IRZ9 (GDSL esterase/lipase At5g03610-like OS=Cucurbita maxima OX=3661 GN=LOC111477899 PE=3 SV=1)

HSP 1 Score: 602.8 bits (1553), Expect = 9.1e-169
Identity = 287/356 (80.62%), Postives = 315/356 (88.48%), Query Frame = 0

Query: 1   MDFVLRLLFSLLLLISLFSGRVSAGGVYNHHHHHQRHRLRPTKLFVFGDSYVDTGNILIP 60
           MDFVLRLLFSLLLLISLFSGR SAGGVYNHHH   RHR RPTKLFVFGDSY DTGNIL P
Sbjct: 2   MDFVLRLLFSLLLLISLFSGRGSAGGVYNHHHQQLRHRFRPTKLFVFGDSYADTGNILFP 61

Query: 61  LSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYVGLKSPIPFTVQNRVGLRRFKETGVN 120
           +S   QFPYGITFPGKP GRFSDGRVLTDFAAR+VGLKSPIPF+V+NRVGLRRFKETGVN
Sbjct: 62  ISPGMQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKETGVN 121

Query: 121 FAFGGTGVFDTSFPFPNMTTQIDFFQQLQHGESLSTKTDNQFSAALVSVSGNDYSLDIIT 180
           FAFGGTGVFDT +P PNMTTQIDFFQ L+ G+S+ T TD Q S ALVS SGNDYS     
Sbjct: 122 FAFGGTGVFDTEYPLPNMTTQIDFFQHLRQGDSILTNTDAQSSVALVSASGNDYSFYFAM 181

Query: 181 NGPKQGLKPFINTVVNQIIVDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNETI 240
           N  +QGLKPFI +VVNQI+V+LKRIG LGVKKIAVTGLGPLGC P FTAPSS+K+CNE+I
Sbjct: 182 NTSRQGLKPFIISVVNQIMVNLKRIGGLGVKKIAVTGLGPLGCFPVFTAPSSYKQCNESI 241

Query: 241 NSFVKFHNFLLKKAVKKLNQETKHSSKVFVLDMYGAFMSIIEGEKRYNNGVFKTPLKPCC 300
           NS V+FHNFLLK+ VKKLNQETKHSSKVF++D+YGAFMSII+G KRYNNG FKTPLKPCC
Sbjct: 242 NSLVQFHNFLLKEGVKKLNQETKHSSKVFIVDVYGAFMSIIQGRKRYNNGEFKTPLKPCC 301

Query: 301 FGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAFSALKAFRNHF 357
           +GV SGF CGSVDGEGK+MFVLC+D K AF+WD+VHPTQQGWAAAF+A K+FRNHF
Sbjct: 302 YGVGSGFSCGSVDGEGKQMFVLCEDRKAAFFWDSVHPTQQGWAAAFAAFKSFRNHF 357

BLAST of CmoCh03G011560.1 vs. NCBI nr
Match: XP_022925652.1 (GDSL esterase/lipase At5g03610-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 732.6 bits (1890), Expect = 1.6e-207
Identity = 356/356 (100.00%), Postives = 356/356 (100.00%), Query Frame = 0

Query: 1   MDFVLRLLFSLLLLISLFSGRVSAGGVYNHHHHHQRHRLRPTKLFVFGDSYVDTGNILIP 60
           MDFVLRLLFSLLLLISLFSGRVSAGGVYNHHHHHQRHRLRPTKLFVFGDSYVDTGNILIP
Sbjct: 1   MDFVLRLLFSLLLLISLFSGRVSAGGVYNHHHHHQRHRLRPTKLFVFGDSYVDTGNILIP 60

Query: 61  LSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYVGLKSPIPFTVQNRVGLRRFKETGVN 120
           LSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYVGLKSPIPFTVQNRVGLRRFKETGVN
Sbjct: 61  LSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYVGLKSPIPFTVQNRVGLRRFKETGVN 120

Query: 121 FAFGGTGVFDTSFPFPNMTTQIDFFQQLQHGESLSTKTDNQFSAALVSVSGNDYSLDIIT 180
           FAFGGTGVFDTSFPFPNMTTQIDFFQQLQHGESLSTKTDNQFSAALVSVSGNDYSLDIIT
Sbjct: 121 FAFGGTGVFDTSFPFPNMTTQIDFFQQLQHGESLSTKTDNQFSAALVSVSGNDYSLDIIT 180

Query: 181 NGPKQGLKPFINTVVNQIIVDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNETI 240
           NGPKQGLKPFINTVVNQIIVDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNETI
Sbjct: 181 NGPKQGLKPFINTVVNQIIVDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNETI 240

Query: 241 NSFVKFHNFLLKKAVKKLNQETKHSSKVFVLDMYGAFMSIIEGEKRYNNGVFKTPLKPCC 300
           NSFVKFHNFLLKKAVKKLNQETKHSSKVFVLDMYGAFMSIIEGEKRYNNGVFKTPLKPCC
Sbjct: 241 NSFVKFHNFLLKKAVKKLNQETKHSSKVFVLDMYGAFMSIIEGEKRYNNGVFKTPLKPCC 300

Query: 301 FGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAFSALKAFRNHF 357
           FGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAFSALKAFRNHF
Sbjct: 301 FGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAFSALKAFRNHF 356

BLAST of CmoCh03G011560.1 vs. NCBI nr
Match: KAG7034510.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 700.3 bits (1806), Expect = 8.7e-198
Identity = 342/347 (98.56%), Postives = 345/347 (99.42%), Query Frame = 0

Query: 1   MDFVLRLLFSLLLLISLFSGRVSAGGVYN-HHHHHQRHRLRPTKLFVFGDSYVDTGNILI 60
           MD VLRLLFSLLLLISLFSGRVSAGGVYN HHHHHQRHRLRPTKLFVFGDSYVDTGNILI
Sbjct: 1   MDSVLRLLFSLLLLISLFSGRVSAGGVYNHHHHHHQRHRLRPTKLFVFGDSYVDTGNILI 60

Query: 61  PLSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYVGLKSPIPFTVQNRVGLRRFKETGV 120
           PLSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYVGLKSPIPF+V+NRVGLRRFKETGV
Sbjct: 61  PLSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYVGLKSPIPFSVRNRVGLRRFKETGV 120

Query: 121 NFAFGGTGVFDTSFPFPNMTTQIDFFQQLQHGESLSTKTDNQFSAALVSVSGNDYSLDII 180
           NFAFGGTGVFDTSFPFPNMTTQIDFFQQLQHGESLSTKTDNQFSAALVSVSGNDYSLDII
Sbjct: 121 NFAFGGTGVFDTSFPFPNMTTQIDFFQQLQHGESLSTKTDNQFSAALVSVSGNDYSLDII 180

Query: 181 TNGPKQGLKPFINTVVNQIIVDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNET 240
           TNGPKQGLKPFINTVVNQII+DLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNET
Sbjct: 181 TNGPKQGLKPFINTVVNQIIMDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNET 240

Query: 241 INSFVKFHNFLLKKAVKKLNQETKHSSKVFVLDMYGAFMSIIEGEKRYNNGVFKTPLKPC 300
           INSFVKFHNFLLKKAVKKLNQETKHSSKVFVLDMYGAFMSIIEGEKRYNNGVFKTPLKPC
Sbjct: 241 INSFVKFHNFLLKKAVKKLNQETKHSSKVFVLDMYGAFMSIIEGEKRYNNGVFKTPLKPC 300

Query: 301 CFGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAF 347
           CFGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAF
Sbjct: 301 CFGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAF 347

BLAST of CmoCh03G011560.1 vs. NCBI nr
Match: XP_022978367.1 (GDSL esterase/lipase At5g03610-like [Cucurbita maxima])

HSP 1 Score: 698.4 bits (1801), Expect = 3.3e-197
Identity = 341/356 (95.79%), Postives = 347/356 (97.47%), Query Frame = 0

Query: 1   MDFVLRLLFSLLLLISLFSGRVSAGGVYNHHHHHQRHRLRPTKLFVFGDSYVDTGNILIP 60
           MDFVLRLLFSL LLISLFSGR SAGGVYN HHHHQRHRLRPTKLFVFGDSYVDTGNILIP
Sbjct: 1   MDFVLRLLFSLFLLISLFSGRGSAGGVYN-HHHHQRHRLRPTKLFVFGDSYVDTGNILIP 60

Query: 61  LSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYVGLKSPIPFTVQNRVGLRRFKETGVN 120
           LSSAQ FPYGITFP KPSGRFSDGRVLTDFAARYVGLKSPIPF+++NRVGLRR KETGVN
Sbjct: 61  LSSAQSFPYGITFPRKPSGRFSDGRVLTDFAARYVGLKSPIPFSIRNRVGLRRLKETGVN 120

Query: 121 FAFGGTGVFDTSFPFPNMTTQIDFFQQLQHGESLSTKTDNQFSAALVSVSGNDYSLDIIT 180
           FAFGGTGVFDTSFPFPNMTTQIDFFQQLQHG SLSTKTDNQFSAALVSVSGNDYSLDIIT
Sbjct: 121 FAFGGTGVFDTSFPFPNMTTQIDFFQQLQHGHSLSTKTDNQFSAALVSVSGNDYSLDIIT 180

Query: 181 NGPKQGLKPFINTVVNQIIVDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNETI 240
           NGPKQGLKPFINTVVNQIIVDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNE+I
Sbjct: 181 NGPKQGLKPFINTVVNQIIVDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNESI 240

Query: 241 NSFVKFHNFLLKKAVKKLNQETKHSSKVFVLDMYGAFMSIIEGEKRYNNGVFKTPLKPCC 300
           NSFVKFHNFLLKKAVKKLNQETKHSSKVFVLD+YGAFMSIIEG KRYNNG FKTPLKPCC
Sbjct: 241 NSFVKFHNFLLKKAVKKLNQETKHSSKVFVLDVYGAFMSIIEGGKRYNNGEFKTPLKPCC 300

Query: 301 FGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAFSALKAFRNHF 357
           FGVKSGF+CGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAFSALKAFRNHF
Sbjct: 301 FGVKSGFNCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAFSALKAFRNHF 355

BLAST of CmoCh03G011560.1 vs. NCBI nr
Match: KAG6604359.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 697.2 bits (1798), Expect = 7.3e-197
Identity = 335/337 (99.41%), Postives = 335/337 (99.41%), Query Frame = 0

Query: 20  GRVSAGGVYNHHHHHQRHRLRPTKLFVFGDSYVDTGNILIPLSSAQQFPYGITFPGKPSG 79
           GRVSAGGVYNHHHH QRHRLRPTKLFVFGDSYVDTGNILIPLSSAQQFPYGITFPGKPSG
Sbjct: 547 GRVSAGGVYNHHHHQQRHRLRPTKLFVFGDSYVDTGNILIPLSSAQQFPYGITFPGKPSG 606

Query: 80  RFSDGRVLTDFAARYVGLKSPIPFTVQNRVGLRRFKETGVNFAFGGTGVFDTSFPFPNMT 139
           RFSDGRVLTDFAARYVGLKSPIPFTVQNRVGLRR KETGVNFAFGGTGVFDTSFPFPNMT
Sbjct: 607 RFSDGRVLTDFAARYVGLKSPIPFTVQNRVGLRRVKETGVNFAFGGTGVFDTSFPFPNMT 666

Query: 140 TQIDFFQQLQHGESLSTKTDNQFSAALVSVSGNDYSLDIITNGPKQGLKPFINTVVNQII 199
           TQIDFFQQLQHGESLSTKTDNQFSAALVSVSGNDYSLDIITNGPKQGLKPFINTVVNQII
Sbjct: 667 TQIDFFQQLQHGESLSTKTDNQFSAALVSVSGNDYSLDIITNGPKQGLKPFINTVVNQII 726

Query: 200 VDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNETINSFVKFHNFLLKKAVKKLN 259
           VDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNETINSFVKFHNFLLKKAVKKLN
Sbjct: 727 VDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNETINSFVKFHNFLLKKAVKKLN 786

Query: 260 QETKHSSKVFVLDMYGAFMSIIEGEKRYNNGVFKTPLKPCCFGVKSGFDCGSVDGEGKKM 319
           QETKHSSKVFVLDMYGAFMSIIEGEKRYNNGVFKTPLKPCCFGVKSGFDCGSVDGEGKKM
Sbjct: 787 QETKHSSKVFVLDMYGAFMSIIEGEKRYNNGVFKTPLKPCCFGVKSGFDCGSVDGEGKKM 846

Query: 320 FVLCKDPKKAFYWDAVHPTQQGWAAAFSALKAFRNHF 357
           FVLCKDPKKAFYWDAVHPTQQGWAAAFSALKAFRNHF
Sbjct: 847 FVLCKDPKKAFYWDAVHPTQQGWAAAFSALKAFRNHF 883

BLAST of CmoCh03G011560.1 vs. NCBI nr
Match: KAG6604360.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 631.3 bits (1627), Expect = 4.9e-177
Identity = 302/356 (84.83%), Postives = 324/356 (91.01%), Query Frame = 0

Query: 1   MDFVLRLLFSLLLLISLFSGRVSAGGVYNHHHHHQRHRLRPTKLFVFGDSYVDTGNILIP 60
           MDFVLRLLFSLLLLISLFSGR SAGGVYNHHHHH RHR RPTKLFVFGDSY DTGNIL P
Sbjct: 2   MDFVLRLLFSLLLLISLFSGRGSAGGVYNHHHHHLRHRFRPTKLFVFGDSYADTGNILFP 61

Query: 61  LSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYVGLKSPIPFTVQNRVGLRRFKETGVN 120
           +S A QFPYGITFPGKP GRFSDGRVLTDFAAR+VGLKSPIP++V++RVGLRRFKETGVN
Sbjct: 62  ISPALQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPYSVRSRVGLRRFKETGVN 121

Query: 121 FAFGGTGVFDTSFPFPNMTTQIDFFQQLQHGESLSTKTDNQFSAALVSVSGNDYSLDIIT 180
           FAFGGTGVFDT +P PNMTTQIDFFQQL+HG+S+ T TD Q S ALVS SGNDYS     
Sbjct: 122 FAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSIFTNTDVQSSVALVSASGNDYSFYFAM 181

Query: 181 NGPKQGLKPFINTVVNQIIVDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNETI 240
           N  +QGLKPFI +VVNQIIV+LKRIG LGVKKIAVTGLGPLGCLP FTAPSS+KKCNETI
Sbjct: 182 NSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKKCNETI 241

Query: 241 NSFVKFHNFLLKKAVKKLNQETKHSSKVFVLDMYGAFMSIIEGEKRYNNGVFKTPLKPCC 300
           NS V+FHNFLLK+AVKKLNQETKHSSKVF++D+YGAFMSIIEG KRYNNG FKTPLKPCC
Sbjct: 242 NSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGAFMSIIEGRKRYNNGEFKTPLKPCC 301

Query: 301 FGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAFSALKAFRNHF 357
           FGVKSGFDCGSVDGEGKKMFVLCKD K AF+WD+VHPTQQGWAAAFSA K+FRNHF
Sbjct: 302 FGVKSGFDCGSVDGEGKKMFVLCKDRKAAFFWDSVHPTQQGWAAAFSAFKSFRNHF 357

BLAST of CmoCh03G011560.1 vs. TAIR 10
Match: AT5G03610.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 355.9 bits (912), Expect = 3.7e-98
Identity = 182/354 (51.41%), Postives = 243/354 (68.64%), Query Frame = 0

Query: 1   MDFVLRLLFSLLLLI--SLFSGRVSAGGVYNHHHHHQRHRLRPTKLFVFGDSYVDTGNIL 60
           MD +++L F L + +  SL  G ++  GV   + +H  +  RPTKLFVFGDSY DTGNI 
Sbjct: 1   MDSLIKLFFCLFIFLCTSLLFGEIN--GVEGSNQNHHLYPFRPTKLFVFGDSYADTGNIK 60

Query: 61  IPLSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYVGLKSPIPFTVQNRVGLRRFKETG 120
              SS+ +FPYGITFPGKP+GRFSDGRV TDF A++VG+KSPIP+  ++  G +R  + G
Sbjct: 61  KAFSSSWKFPYGITFPGKPAGRFSDGRVATDFLAKFVGIKSPIPYFWKDYAGKKRL-QYG 120

Query: 121 VNFAFGGTGVFDTSFPFPNMTTQIDFFQQLQHGESLSTKTDNQFSAALVSVSGNDYSLDI 180
           +NFA+GGTGVF+T  P PNMTTQID FQ +     +    +   S ALVSV+GNDYS  I
Sbjct: 121 MNFAYGGTGVFNTQTPLPNMTTQIDIFQNILTTGDIYYPPELTSSVALVSVAGNDYSNFI 180

Query: 181 ITNGPKQGLKPFINTVVNQIIVDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNE 240
             N P      FI  VV+Q  V+L+RI  LGVKKIAV  L PLGCLP FT  +SF++CNE
Sbjct: 181 ALNRPASEFPAFIKQVVDQTEVNLRRIHALGVKKIAVPSLQPLGCLPPFTFVTSFQRCNE 240

Query: 241 TINSFVKFHNFLLKKAVKKLNQETKHSSKVFVLDMYGAFMSIIEGEKRYNNGV--FKTPL 300
           T N+ V  HN LL++ V KLN ETK S+ + +LD+Y AF+++ +  K  N G   F++PL
Sbjct: 241 TQNALVNLHNNLLQQVVAKLNNETKQSTFI-ILDLYNAFLTVFK-NKGSNPGSTRFESPL 300

Query: 301 KPCCFGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAFSALK 351
           KPCC GV   ++CGSVD +G K +++C +PK AF+WD +HPT++GW + +S L+
Sbjct: 301 KPCCVGVSREYNCGSVDEKGVKKYIVCDNPKTAFFWDGLHPTEEGWRSVYSVLR 349

BLAST of CmoCh03G011560.1 vs. TAIR 10
Match: AT3G09930.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 335.1 bits (858), Expect = 6.8e-92
Identity = 173/343 (50.44%), Postives = 230/343 (67.06%), Query Frame = 0

Query: 13  LLISLFSGRVSA---GGVYNHHHHHQRHRLRPTKLFVFGDSYVDTGNILIPLSSAQQFPY 72
           LLISLF    S+   G   ++  H    RLRP +LFVFGDSY DTGNI   LS + + PY
Sbjct: 6   LLISLFLFSFSSFFLGAESDYPQHSNLKRLRPNRLFVFGDSYADTGNIRKSLSDSWKIPY 65

Query: 73  GITFPGKPSGRFSDGRVLTDFAARYVGLKSPIPFTVQNRVGLRRFKETGVNFAFGGTGVF 132
           GITFP KPSGRFSDGRV TDF ARY+G+KSPIP+T ++  G  R    G+N+A+GGTGVF
Sbjct: 66  GITFPQKPSGRFSDGRVATDFLARYLGIKSPIPYTWKDYAGKERLL-YGMNYAYGGTGVF 125

Query: 133 DT-SFPFPNMTTQIDFFQQLQHGESLSTKTDNQFSAALVSVSGNDYSLDIITNGPKQGLK 192
            T   P PNMTTQID+FQ++    ++ + +D   S ALVSV+GNDY+  +    P   L 
Sbjct: 126 KTKDNPLPNMTTQIDYFQRVLAAGNIYSPSDLPSSLALVSVAGNDYATFLALKRPLTELP 185

Query: 193 PFINTVVNQIIVDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNETINSFVKFHN 252
            F+  VV+QI V+  RI +LGV KI +  + PLGCLP+ T  +SF++CN T N+    HN
Sbjct: 186 AFMKQVVDQIAVNAMRIHKLGVNKIVIPSMQPLGCLPSITVFNSFQRCNATDNASTNLHN 245

Query: 253 FLLKKAVKKLNQETKHSSKVFVLDMYGAFMSIIEGEKRYNNGV--FKTPLKPCCFGVKSG 312
           +LL KA+ +LN ETK S+ V VLD Y AF+++ +  K    GV  F  PLKPCC GV S 
Sbjct: 246 YLLHKAIARLNNETKPSTFV-VLDHYNAFLTVFK-NKGPEPGVSRFGNPLKPCCVGVNSS 305

Query: 313 FDCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAFSAL 350
           +DC +VD +G+K +++C+DPK AF+WD  HP+++GW + +S L
Sbjct: 306 YDCSNVDEKGEKKYIICEDPKAAFFWDIFHPSEEGWRSVYSVL 345

BLAST of CmoCh03G011560.1 vs. TAIR 10
Match: AT5G03600.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 258.8 bits (660), Expect = 6.2e-69
Identity = 147/323 (45.51%), Postives = 198/323 (61.30%), Query Frame = 0

Query: 39  LRP---TKLFVFGDSYVDTGNILIPLSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYV 98
           LRP    KLFVFGDSY DTGN     + A   PYGITFPGKPSGR+ DG + TDF  + +
Sbjct: 7   LRPRKVPKLFVFGDSYADTGNTKRD-TEAWAIPYGITFPGKPSGRYCDGLIATDFLEKVL 66

Query: 99  GLKSPIPFTVQNR-VGLRRFKETGVNFAFGGTGVFDTS--FPFPNMTTQIDFFQQL-QHG 158
           G +SP  +    R  GL+R    G+NFAFGG+ + D+S   PFPN+T Q++F   L   G
Sbjct: 67  GAESPYLYRTHGRDKGLKR----GMNFAFGGSKMLDSSPNSPFPNITAQVNFLVDLVLAG 126

Query: 159 ESLSTKTDNQFSAALVSVSGNDYSLDIITNGPKQGLKPFINTVVNQIIVDLKRIGELGVK 218
                 T +  S  L+S +G DY   I  N P  GLK  +  VV+ + V++  +G L  K
Sbjct: 127 RVYGDITPSDVS--LISYAGGDYIYYIDQNRPAAGLKALVEKVVDNLRVNMIVLGGLLFK 186

Query: 219 KIAVTGLGPLGCLPAFTAPSSFKKCNETINSFVKFHNFLLKKAVKKLNQET---KHSSKV 278
           KIAVT L P+GCLP++T+ SSFK CNE+ ++ V+ HN LLKK V KLN+++   K     
Sbjct: 187 KIAVTSLQPIGCLPSYTSASSFKSCNESQSALVELHNKLLKKVVAKLNEQSRVMKKEQHF 246

Query: 279 FVLDMYGAFMSII--EGEKRYNNGVFKTPLKPCCFGVKSGFDCGSVDGEGKKMFVLCKDP 338
           F++D++ AFM+++  +G KR     FK P+K CC G      CG    +G K++ LC DP
Sbjct: 247 FIIDIHNAFMTVMKNKGSKR-----FKNPMKSCCEGY-----CGR-SSDGGKLYTLCDDP 306

Query: 339 KKAFYWDAVHPTQQGWAAAFSAL 350
           K  F+WDAVHPTQ+GW + +S L
Sbjct: 307 KSFFFWDAVHPTQEGWRSIYSVL 311

BLAST of CmoCh03G011560.1 vs. TAIR 10
Match: AT2G36325.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 243.4 bits (620), Expect = 2.7e-64
Identity = 134/313 (42.81%), Postives = 183/313 (58.47%), Query Frame = 0

Query: 40  RPTKLFVFGDSYVDTGNILIPLSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYVGLKS 99
           +PTKLFVFGDSY DTGN    +  + +FP GITFPG P+GRFSDGRV TD+ A+Y+G+++
Sbjct: 44  KPTKLFVFGDSYADTGNTPFLIVPSWRFPNGITFPGIPTGRFSDGRVSTDYLAKYIGVRT 103

Query: 100 PIPFTVQNRVGLRRFKETGVNFAFGGTGVFDTSFPF-PNMTTQIDFFQQLQHGESLSTKT 159
           PI +        R   + G+NFA+GG G F+T F   P  + QID F+QL    ++ +  
Sbjct: 104 PITYKWGKYGRPRLAVKRGMNFAYGGAGAFETMFKLVPTASVQIDSFEQLLM-RNVYSPA 163

Query: 160 DNQFSAALVSVSGNDYSLDIITNGPKQGLKPFINTVVNQIIVDLKRIGELGVKKIAVTGL 219
           D   S A  S+ GNDY      NG ++G       VV QI++D+KRI +LGV+K+ V   
Sbjct: 164 DLNSSVAFFSIIGNDYLTYDRRNGSEEGRSALTRKVVKQILLDVKRIKDLGVRKVLVALS 223

Query: 220 GPLGCLPAFTAPSSFKKCNETINSFVKFHNFLLKKAVKKLN-QETKHSSKVFV-LDMYGA 279
            P  CLP    P   K C +T ++    HN LL+K + KLN +E  ++ K F+ LD+Y A
Sbjct: 224 PPQKCLPKLVTP---KGC-DTNDTSTYLHNSLLRKGLIKLNDKEINNNDKSFMTLDLYNA 283

Query: 280 FMSIIEGEKRYNNGVFKTPLKPCCFGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWDAVH 339
           F++I + +       F  P K CC   K G  CG     GKK++ LC DPK  F+WD VH
Sbjct: 284 FVTIFKNKGVSGVSTFPDPFKACC-ATKRGTFCGDRSLSGKKLYTLCDDPKSFFFWDNVH 343

Query: 340 PTQQGWAAAFSAL 350
            + QGW + FS L
Sbjct: 344 ISDQGWRSVFSLL 350

BLAST of CmoCh03G011560.1 vs. TAIR 10
Match: AT5G03590.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 147.9 bits (372), Expect = 1.5e-35
Identity = 99/295 (33.56%), Postives = 134/295 (45.42%), Query Frame = 0

Query: 60  PLSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYVGLKSPIPFTVQNRVGLRRFKETGV 119
           P + + + PYGITFPGKPSGR+SDG   TDF                             
Sbjct: 3   PTALSWKLPYGITFPGKPSGRYSDGLTATDFL---------------------------- 62

Query: 120 NFAFGGTGVFDTSFP-FPNMTTQIDFFQQLQHGESLST-KTDNQFSAALVSVSGNDYSLD 179
                G+ VFD+     PN++TQ+ F   L     + T   D   S AL+S SG DY   
Sbjct: 63  -----GSEVFDSPVDRSPNISTQVGFLVNLALARRVYTIDGDLASSYALLSYSGTDYYGF 122

Query: 180 IITNGPKQGLKPFINTVVNQIIVDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCN 239
           I  N        F+  +V  I                                     CN
Sbjct: 123 IDQNPNMAAYPAFVEFIVEDI------------------------------------HCN 182

Query: 240 ETINSFVKFHNFLLKKAVKKLNQETKHSSK---VFVLDMYGAFMSIIEGEKRYNNGVFKT 299
           E+ +  V+ HN  LKKAV KLN+E K  +K     ++D++ AFM+I+E   +  N  FK+
Sbjct: 183 ESYSDLVRLHNESLKKAVAKLNKEDKFRTKGDRFVIVDLHKAFMTILE---KKGNKRFKS 220

Query: 300 PLKPCCFGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAFSAL 350
           PLKPCC G     DC  +D +G K + LC DPK AF+WD ++PTQ+GW + +S L
Sbjct: 243 PLKPCCEG-----DCARMDMKGAKKYTLCNDPKSAFFWDEINPTQEGWRSIYSLL 220