CmoCh02G012590.1 (mRNA) Cucurbita moschata (Rifu) v1

BLAST of CmoCh02G012590.1 vs. ExPASy Swiss-Prot
Match: Q9C5V6 (DNA topoisomerase 6 subunit B OS=Arabidopsis thaliana OX=3702 GN=TOP6B PE=1 SV=1)

HSP 1 Score: 1082.0 bits (2797), Expect = 0.0e+00
Identity = 540/662 (81.57%), Postives = 604/662 (91.24%), Query Frame = 0

Query: 12  EPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKSLYTTVRELVENSLDSAES 71
           E KKG SK  +   ES LKQKSPAEFFAENKNIAGFDNPGKSLYTTVRELVEN+LDSAES
Sbjct: 8   ETKKGSSKNSKDSNESKLKQKSPAEFFAENKNIAGFDNPGKSLYTTVRELVENALDSAES 67

Query: 72  ISELPVVEITIEEILKTKFNSMIGLVDRERVDAELYDDYETAKAREKRLAKEARAQEIQA 131
           ISELP VE+TIEEI+K+KFNSMIGL+DRERVD +LYDDYET KAR KRLAKEARA EIQA
Sbjct: 68  ISELPEVEVTIEEIVKSKFNSMIGLIDRERVDTQLYDDYETEKARGKRLAKEARASEIQA 127

Query: 132 KNASLGKKGREPAASKGMKGRGEASFYRVTCRDNGRGMPHDDIPNMFGRVLSGTKYGLKQ 191
           KN + GKK +EP  SK +K RGEAS+Y+VTC+DNG+GMPHDDIPNMFGRVLSGTKYGLKQ
Sbjct: 128 KNLASGKKNKEPGVSKVLKARGEASYYKVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQ 187

Query: 192 TRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNIPHVHVQEKRDN 251
           TRGKFGLGAKMALIWSKMSTGLPIEISSSMK QNY +FCRLDIDIHRNIPH+H+ EK+ N
Sbjct: 188 TRGKFGLGAKMALIWSKMSTGLPIEISSSMKSQNYVTFCRLDIDIHRNIPHIHLHEKKGN 247

Query: 252 KDLWHGAEIQVVIEGNWTTYRSKILYYMRQMAVITPYAQFLFKFVSDTPDKNITIRFSRR 311
           K+ WHGAEIQVVIEGNWTTYRSKIL+YMRQMAVITPYAQFLF+F+S+TP+KN+TI+F+RR
Sbjct: 248 KEKWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFRFISETPEKNVTIKFTRR 307

Query: 312 TDVMPPVPQETKHHPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKSYAERLIGEMGP 371
           TDVMPP+P ETKHHPS+VDLLLIKRLI +T+K++LLQFLQ+EFVN+ K+ A RLIGEMGP
Sbjct: 308 TDVMPPIPIETKHHPSSVDLLLIKRLITDTSKKTLLQFLQNEFVNINKTLAARLIGEMGP 367

Query: 372 EFCPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATY 431
           +F P M+VKS+TSQQ+VRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATY
Sbjct: 368 DFGPSMAVKSVTSQQMVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATY 427

Query: 432 SGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWS 491
           SGSAQVFEGHPFIVEAGVS+GG+DVKQG+NIFRFANRIPLLFEQGADVVTRTALKRINW+
Sbjct: 428 SGSAQVFEGHPFIVEAGVSLGGRDVKQGINIFRFANRIPLLFEQGADVVTRTALKRINWN 487

Query: 492 SYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQCCIQLKSKIVK 551
           SYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIA+AVKSAIQQCCIQLKSKIVK
Sbjct: 488 SYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIATAVKSAIQQCCIQLKSKIVK 547

Query: 552 KIQAREQQERKRNLSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAEILSKVSARVIT 611
           ++QAREQQERKR+LSRYIPDAT AVY VLK+M   H +K+KR+  E+  +L KVS ++IT
Sbjct: 548 RLQAREQQERKRSLSRYIPDATGAVYEVLKQMTEEHKTKRKRYGEEDIVMLDKVSKQIIT 607

Query: 612 NETLRDKLAQHVEQVDHEMAMEYATQSGASAESKEEIYIQSLDAT-DNFTDFKSPLFVFR 671
            ETL++KLA+HVEQVD+EMA+EYATQSG S E +E IY+Q LD    NF D  SP FVFR
Sbjct: 608 KETLKEKLAEHVEQVDYEMALEYATQSGVSEEPRENIYLQHLDPNKSNFIDLHSPTFVFR 667

Query: 672 LV 673
           L+
Sbjct: 668 LM 669

BLAST of CmoCh02G012590.1 vs. ExPASy Swiss-Prot
Match: B8BDQ4 (DNA topoisomerase 6 subunit B OS=Oryza sativa subsp. indica OX=39946 GN=TOP6B PE=1 SV=1)

HSP 1 Score: 1035.8 bits (2677), Expect = 2.2e-301
Identity = 510/648 (78.70%), Postives = 588/648 (90.74%), Query Frame = 0

Query: 25  KESILKQKSPAEFFAENKNIAGFDNPGKSLYTTVRELVENSLDSAESISELPVVEITIEE 84
           + S+LKQKSPAEFFAENKNIAGFDNPGKSLYTT+RELVEN+LDSAESISELP +EI IEE
Sbjct: 49  ESSLLKQKSPAEFFAENKNIAGFDNPGKSLYTTMRELVENALDSAESISELPDIEIIIEE 108

Query: 85  ILKTKFNSMIGLVDRERVDAELYDDYETAKAREKRLAKEARAQEIQAKNASLGKKGREPA 144
           I K+KFN+MIGLVDR+R+D ELYDD+E+AKAREKRLAKEAR QE QAKNA+LGKK +E  
Sbjct: 109 ITKSKFNTMIGLVDRQRIDEELYDDFESAKAREKRLAKEARFQETQAKNAALGKKVKEAP 168

Query: 145 ASKGMKGRGEASFYRVTCRDNGRGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMAL 204
           A++G KGRGEA+F+RVTC+DNGRGMPHDDIPNM GRVLSGTKYGL+QTRGKFGLGAKMAL
Sbjct: 169 AARG-KGRGEAAFFRVTCKDNGRGMPHDDIPNMLGRVLSGTKYGLRQTRGKFGLGAKMAL 228

Query: 205 IWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNIPHVHVQEKRDNKDLWHGAEIQVVI 264
           IWSKMSTGLPIEI SSMKGQN+ SFCRLDIDIH+N+PHVH+ EKR+NKD WHGAE+QV+I
Sbjct: 229 IWSKMSTGLPIEIKSSMKGQNFISFCRLDIDIHKNVPHVHLHEKRENKDRWHGAELQVII 288

Query: 265 EGNWTTYRSKILYYMRQMAVITPYAQFLFKFVSDTPDKNITIRFSRRTDVMPPVPQETKH 324
           EGNWTT+RSKIL+YMRQMAVITPYAQFLF+F+SD+PDKN+TI+F+RRTDVMPP+P +TKH
Sbjct: 289 EGNWTTHRSKILHYMRQMAVITPYAQFLFRFLSDSPDKNLTIQFARRTDVMPPIPLQTKH 348

Query: 325 HPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKSYAERLIGEMGPEFCPKMSVKSLTS 384
           HPSAVDLLLIKRLI+ET KQ+LLQFLQHEFVN+ KS+AERLIGEMGP+F  K +VKSLTS
Sbjct: 349 HPSAVDLLLIKRLISETTKQNLLQFLQHEFVNISKSHAERLIGEMGPDFSAKTTVKSLTS 408

Query: 385 QQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATYSGSAQVFEGHPFI 444
           QQ+VRIHQLFRQAKFDDPSG+CLSPAGEYNLRLGIIKELHPD+VAT++ S QVFEGHPFI
Sbjct: 409 QQLVRIHQLFRQAKFDDPSGNCLSPAGEYNLRLGIIKELHPDLVATHASSPQVFEGHPFI 468

Query: 445 VEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWSSYKINQTQDKIGV 504
           VEAG+SIGGKDVK GLNIFR+ANRIPLLFEQGADV+TRTALKRINWSSYKINQ QDKIGV
Sbjct: 469 VEAGISIGGKDVKHGLNIFRYANRIPLLFEQGADVITRTALKRINWSSYKINQQQDKIGV 528

Query: 505 FVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQCCIQLKSKIVKKIQAREQQERKRN 564
           FVSIVSTKIPFKGTGKEYIGDDI+EIASAV+SA++QCC+QLKSKIVKK+QARE+Q+RKRN
Sbjct: 529 FVSIVSTKIPFKGTGKEYIGDDITEIASAVQSALKQCCLQLKSKIVKKLQARERQDRKRN 588

Query: 565 LSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAEILSKVSARVITNETLRDKLAQHVE 624
           L+RYIPD   A+   L E+A     K+ R+D E+ E+L KV++  +T  T RD L QHVE
Sbjct: 589 LNRYIPDVARAIMETLGEIADESPPKRPRYDKEDEELLEKVNSEEVTEMTFRDCLTQHVE 648

Query: 625 QVDHEMAMEYATQSGASAESKEEIYIQSLDATDNFTDFKSPLFVFRLV 673
           QVD+EMA+EYA QSG S E +E +Y+ SL+ +  F DF+SP+FVFR +
Sbjct: 649 QVDYEMALEYAMQSGVSEEPREALYLNSLEGSYKFIDFQSPVFVFRFI 695

BLAST of CmoCh02G012590.1 vs. ExPASy Swiss-Prot
Match: Q6H442 (DNA topoisomerase 6 subunit B OS=Oryza sativa subsp. japonica OX=39947 GN=TOP6B PE=1 SV=1)

HSP 1 Score: 1033.1 bits (2670), Expect = 1.4e-300
Identity = 509/648 (78.55%), Postives = 587/648 (90.59%), Query Frame = 0

Query: 25  KESILKQKSPAEFFAENKNIAGFDNPGKSLYTTVRELVENSLDSAESISELPVVEITIEE 84
           + S+LKQKSPAEFFAENKNIAGFDNPGKSLYTT+RELVEN+LDSAESISELP +EI IEE
Sbjct: 49  ESSLLKQKSPAEFFAENKNIAGFDNPGKSLYTTMRELVENALDSAESISELPDIEIIIEE 108

Query: 85  ILKTKFNSMIGLVDRERVDAELYDDYETAKAREKRLAKEARAQEIQAKNASLGKKGREPA 144
           I K+KFN+MIGLVDR+R+D ELYDD+E+AKAREKRLAKEAR QE QAKNA+LGKK +E  
Sbjct: 109 ITKSKFNTMIGLVDRQRIDEELYDDFESAKAREKRLAKEARFQETQAKNAALGKKVKEAP 168

Query: 145 ASKGMKGRGEASFYRVTCRDNGRGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMAL 204
           A++G KGRGEA+F+RVTC+DNGRGMPHDDIPNM GRVLSGTKYGL+QTRGKFGLGAKMAL
Sbjct: 169 AARG-KGRGEAAFFRVTCKDNGRGMPHDDIPNMLGRVLSGTKYGLRQTRGKFGLGAKMAL 228

Query: 205 IWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNIPHVHVQEKRDNKDLWHGAEIQVVI 264
           IWSKMSTGLPIEI SSMKGQN+ SFC LDIDIH+N+PHVH+ EKR+NKD WHGAE+QV+I
Sbjct: 229 IWSKMSTGLPIEIKSSMKGQNFISFCLLDIDIHKNVPHVHLHEKRENKDRWHGAELQVII 288

Query: 265 EGNWTTYRSKILYYMRQMAVITPYAQFLFKFVSDTPDKNITIRFSRRTDVMPPVPQETKH 324
           EGNWTT+RSKIL+YMRQMAVITPYAQFLF+F+SD+PDKN+TI+F+RRTDVMPP+P +TKH
Sbjct: 289 EGNWTTHRSKILHYMRQMAVITPYAQFLFRFLSDSPDKNLTIQFARRTDVMPPIPLQTKH 348

Query: 325 HPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKSYAERLIGEMGPEFCPKMSVKSLTS 384
           HPSAVDLLLIKRLI+ET KQ+LLQFLQHEFVN+ KS+AERLIGEMGP+F  K +VKSLTS
Sbjct: 349 HPSAVDLLLIKRLISETTKQNLLQFLQHEFVNISKSHAERLIGEMGPDFSAKTTVKSLTS 408

Query: 385 QQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATYSGSAQVFEGHPFI 444
           QQ+VRIHQLFRQAKFDDPSG+CLSPAGEYNLRLGIIKELHPD+VAT++ S QVFEGHPFI
Sbjct: 409 QQLVRIHQLFRQAKFDDPSGNCLSPAGEYNLRLGIIKELHPDLVATHASSPQVFEGHPFI 468

Query: 445 VEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWSSYKINQTQDKIGV 504
           VEAG+SIGGKDVK GLNIFR+ANRIPLLFEQGADV+TRTALKRINWSSYKINQ QDKIGV
Sbjct: 469 VEAGISIGGKDVKHGLNIFRYANRIPLLFEQGADVITRTALKRINWSSYKINQQQDKIGV 528

Query: 505 FVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQCCIQLKSKIVKKIQAREQQERKRN 564
           FVSIVSTKIPFKGTGKEYIGDDI+EIASAV+SA++QCC+QLKSKIVKK+QARE+Q+RKRN
Sbjct: 529 FVSIVSTKIPFKGTGKEYIGDDITEIASAVQSALKQCCLQLKSKIVKKLQARERQDRKRN 588

Query: 565 LSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAEILSKVSARVITNETLRDKLAQHVE 624
           L+RYIPD   A+   L E+A     K+ R+D E+ E+L KV++  +T  T RD L QHVE
Sbjct: 589 LNRYIPDVARAIMETLGEIADESPPKRPRYDKEDEELLEKVNSEEVTEMTFRDCLTQHVE 648

Query: 625 QVDHEMAMEYATQSGASAESKEEIYIQSLDATDNFTDFKSPLFVFRLV 673
           QVD+EMA+EYA QSG S E +E +Y+ SL+ +  F DF+SP+FVFR +
Sbjct: 649 QVDYEMALEYAMQSGVSEEPREALYLNSLEGSYKFIDFQSPVFVFRFI 695

BLAST of CmoCh02G012590.1 vs. ExPASy Swiss-Prot
Match: Q9YE64 (Type 2 DNA topoisomerase 6 subunit B OS=Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) OX=272557 GN=top6B PE=3 SV=2)

HSP 1 Score: 305.4 bits (781), Expect = 1.5e-81
Identity = 178/545 (32.66%), Postives = 288/545 (52.84%), Query Frame = 0

Query: 30  KQKSPAEFFAENKNIAGFDNPGKSLYTTVRELVENSLDSAESISELPVVEITIEEILKTK 89
           ++ S AEFFA+NK +AGF NP ++LY T+RELVENSLD+ ++   LP + I+I +I    
Sbjct: 29  REMSVAEFFAKNKELAGFANPTRALYQTIRELVENSLDATDAKGILPWIHISIRQI---- 88

Query: 90  FNSMIGLVDRERVDAELYDDYETAKAREKRLAKEARAQEIQAKNASLGKKGREPAASKGM 149
                                                                    +G 
Sbjct: 89  ---------------------------------------------------------EGS 148

Query: 150 KGRGEASFYRVTCRDNGRGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKM 209
           +GRG    Y VT  DNG G+P   +   FG+VL  +KY ++QTRG +GLG K A+++ +M
Sbjct: 149 EGRGGRPRYTVTVEDNGIGVPVTSMAMAFGKVLFSSKYVIRQTRGMYGLGVKAAILYGQM 208

Query: 210 STGLPIEISSSMKGQNYTSFCRLDIDIHRNIPHVHVQEKRDNKDLWHGAEIQVVIEGNWT 269
           + G P+E+ S+ KG  Y    +L ID+ RN P +  + + + +  WHG  + + +EG+W+
Sbjct: 209 TAGTPVEVYSATKGSQYVYMMKLVIDVQRNEPRILERGEWNKRGSWHGTRVTLSLEGDWS 268

Query: 270 TYRSKILYYMRQMAVITPYAQFLFKFVSDTPDKNITIRFSRRTDVMPPVPQETKHHPSAV 329
             +SKIL Y+++ AVI PYA+ +     +TPD  I   F R T  +P  P+E+K HP  V
Sbjct: 269 RAKSKILEYIKRTAVIAPYAEIIL----ETPDGEI-YYFPRSTRKLPKPPRESKPHPHGV 328

Query: 330 DLLLIKRLIAETAKQSLLQFLQHEFVNLGKSYAERLIGEMG--PEFCPKMSVKSLTSQQI 389
           D+  +K ++  T   +L +FL  EF ++G   A+  + + G  P+  P+  +K    + +
Sbjct: 329 DIEQLKSMLRSTRASTLREFLVKEFQSIGDKTAQDFLEKYGLDPDLKPRELLKKGRDKLL 388

Query: 390 VRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATYSGSAQVFEGHPFIVEA 449
            R+     +  F  P  D LSP G   + +G+ +  +P+ V   S S + + GHPFIVE 
Sbjct: 389 RRLADALMEYPFRAPKSDYLSPIGSDIIEIGLRRMFNPEWVGAVSRSPKAYRGHPFIVEV 448

Query: 450 GVSIGGK-DVKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWSSYKINQTQDKIGVFV 509
           G++ GG  + +    + R+AN+IPLL+E+  D V+   +  INW  Y ++     + V V
Sbjct: 449 GIAYGGGIEARSEPLLLRYANKIPLLYEERED-VSYKVVSSINWRQYNVDFPAPLV-VLV 504

Query: 510 SIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQCCIQLKSKIVKKIQAREQQERKRNLS 569
            I STK+P+KG GKE +  D+ EI + +++A+Q+   +L+  + +K +  E   R   L+
Sbjct: 509 HIASTKVPYKGVGKESV-SDVPEIEAEIRNAVQEVARRLRLYLSRKAREEEAIRRSVTLA 504

Query: 570 RYIPD 572
           +YIP+
Sbjct: 569 KYIPE 504

BLAST of CmoCh02G012590.1 vs. ExPASy Swiss-Prot
Match: C3N5B3 (Type 2 DNA topoisomerase 6 subunit B OS=Sulfolobus islandicus (strain M.16.27) OX=427318 GN=top6B PE=3 SV=1)

HSP 1 Score: 296.6 bits (758), Expect = 7.2e-79
Identity = 186/554 (33.57%), Postives = 298/554 (53.79%), Query Frame = 0

Query: 33  SPAEFFAENKNIAGFDNPGKSLYTTVRELVENSLDSAESISELPVVEITIEEILKTKFNS 92
           SPAEFF  N  +AGF NP ++LY TVREL+ENSLD+ +    LP ++ITI+         
Sbjct: 11  SPAEFFKRNPELAGFPNPARALYQTVRELIENSLDATDVHGILPNIKITID--------- 70

Query: 93  MIGLVDRERVDAELYDDYETAKAREKRLAKEARAQEIQAKNASLGKKGREPAASKGMKGR 152
              L+D  R                                                   
Sbjct: 71  ---LIDEAR--------------------------------------------------- 130

Query: 153 GEASFYRVTCRDNGRGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTG 212
                Y+V   DNG G+P  ++PN FGRVL  +KY  +QTRG +GLG K A+++S+M   
Sbjct: 131 ---QIYKVNVVDNGIGIPPQEVPNAFGRVLYSSKYVNRQTRGMYGLGVKAAVLYSQMHQD 190

Query: 213 LPIEISSSMKGQNYTSFCRLDIDIHRNIPHVHVQEKRDNKDLWHGAEIQVVIEGNWTTYR 272
            PIEI +S          +L IDI++N P +  +   +N   +HG  + + I G+W   +
Sbjct: 191 KPIEIETSPANSKRIYTFKLKIDINKNEPIIVERGSVENTRGFHGTSVAISIPGDWPKAK 250

Query: 273 SKILYYMRQMAVITPYAQFLFKFVSDTPDKNITIRFSRRTDVMPPVPQETKHHPSAVDLL 332
           S+I  Y+++  +ITPYA+F+FK     P+ N+T  + R T+ +P  PQE K HP  VD  
Sbjct: 251 SRIYEYIKRTYIITPYAEFIFK----DPEGNVTY-YPRLTNKIPKPPQEVKPHPYGVDRE 310

Query: 333 LIKRLIAETAKQ-SLLQFLQHEFVNLGKSYAERLIGEMGPEFCPKMSVKSLTSQQIVRIH 392
            IK LI    +  ++ +FL +EF ++G + A++++   G +  P   VK+LT ++I R+ 
Sbjct: 311 EIKILINNLKRDYTIKEFLVNEFQSIGDTTADKILELAGLK--PNKKVKNLTEEEITRLV 370

Query: 393 QLFRQAK-FDDPSGDCLSPAGEYNLRLGIIKELHPDMVATYSGSAQVFEGHPFIVEAGVS 452
           + F++ + F  PS D LS  GE  + LG+ K  +PD  A+ +   + ++GHPFIVEAGV+
Sbjct: 371 ETFKKYEDFRSPSADSLSVIGEDLIELGLKKIFNPDFAASITRKPKAYQGHPFIVEAGVA 430

Query: 453 IGGK-DVKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWSSYKINQTQDKIGVFVSIV 512
            GG   V +   + R+AN+IPL++++ +DV+ +  ++ ++W  Y I   Q ++ V V + 
Sbjct: 431 FGGSIPVGEEPIVLRYANKIPLIYDEKSDVIWK-VVEELDWKRYGIESDQYQMVVMVHLC 486

Query: 513 STKIPFKGTGKEYIGDDISEIASAVKSAIQQCCIQLKSKIVKKIQAREQQERKRNL---S 572
           STKIP+K  GKE I  ++ +I   +K+A+ +   +LK  + +K   R++QE K+ L    
Sbjct: 491 STKIPYKSAGKESIA-EVEDIEKEIKNALMEVARKLKQYLSEK---RKEQEAKKKLLAYL 486

Query: 573 RYIPDATNAVYNVL 581
           +YIP+ + ++   L
Sbjct: 551 KYIPEVSRSLATFL 486

BLAST of CmoCh02G012590.1 vs. ExPASy TrEMBL
Match: A0A6J1H1C2 (DNA topoisomerase 6 subunit B OS=Cucurbita moschata OX=3662 GN=LOC111459466 PE=3 SV=1)

HSP 1 Score: 1316.2 bits (3405), Expect = 0.0e+00
Identity = 673/673 (100.00%), Postives = 673/673 (100.00%), Query Frame = 0

Query: 1   MEIGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKSLYTTVRE 60
           MEIGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKSLYTTVRE
Sbjct: 1   MEIGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKSLYTTVRE 60

Query: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDAELYDDYETAKAREKRL 120
           LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDAELYDDYETAKAREKRL
Sbjct: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDAELYDDYETAKAREKRL 120

Query: 121 AKEARAQEIQAKNASLGKKGREPAASKGMKGRGEASFYRVTCRDNGRGMPHDDIPNMFGR 180
           AKEARAQEIQAKNASLGKKGREPAASKGMKGRGEASFYRVTCRDNGRGMPHDDIPNMFGR
Sbjct: 121 AKEARAQEIQAKNASLGKKGREPAASKGMKGRGEASFYRVTCRDNGRGMPHDDIPNMFGR 180

Query: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240
           VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI
Sbjct: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240

Query: 241 PHVHVQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILYYMRQMAVITPYAQFLFKFVSDTP 300
           PHVHVQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILYYMRQMAVITPYAQFLFKFVSDTP
Sbjct: 241 PHVHVQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILYYMRQMAVITPYAQFLFKFVSDTP 300

Query: 301 DKNITIRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKS 360
           DKNITIRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKS
Sbjct: 301 DKNITIRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKS 360

Query: 361 YAERLIGEMGPEFCPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420
           YAERLIGEMGPEFCPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII
Sbjct: 361 YAERLIGEMGPEFCPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420

Query: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480
           KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV
Sbjct: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480

Query: 481 TRTALKRINWSSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540
           TRTALKRINWSSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ
Sbjct: 481 TRTALKRINWSSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540

Query: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAE 600
           CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAE
Sbjct: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAE 600

Query: 601 ILSKVSARVITNETLRDKLAQHVEQVDHEMAMEYATQSGASAESKEEIYIQSLDATDNFT 660
           ILSKVSARVITNETLRDKLAQHVEQVDHEMAMEYATQSGASAESKEEIYIQSLDATDNFT
Sbjct: 601 ILSKVSARVITNETLRDKLAQHVEQVDHEMAMEYATQSGASAESKEEIYIQSLDATDNFT 660

Query: 661 DFKSPLFVFRLVH 674
           DFKSPLFVFRLVH
Sbjct: 661 DFKSPLFVFRLVH 673

BLAST of CmoCh02G012590.1 vs. ExPASy TrEMBL
Match: A0A6J1K0W3 (DNA topoisomerase 6 subunit B OS=Cucurbita maxima OX=3661 GN=LOC111490712 PE=3 SV=1)

HSP 1 Score: 1294.3 bits (3348), Expect = 0.0e+00
Identity = 662/673 (98.37%), Postives = 666/673 (98.96%), Query Frame = 0

Query: 1   MEIGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKSLYTTVRE 60
           MEIGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGK LYTTVRE
Sbjct: 1   MEIGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60

Query: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDAELYDDYETAKAREKRL 120
           LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVD ELYDDYETAKAREKRL
Sbjct: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120

Query: 121 AKEARAQEIQAKNASLGKKGREPAASKGMKGRGEASFYRVTCRDNGRGMPHDDIPNMFGR 180
            KEARAQEI AKNASLGKKGREPA SKGMKGRGEASFYRVTC+DNGRGMPHDDIPNMFGR
Sbjct: 121 VKEARAQEILAKNASLGKKGREPAVSKGMKGRGEASFYRVTCKDNGRGMPHDDIPNMFGR 180

Query: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240
           VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDI+RNI
Sbjct: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDINRNI 240

Query: 241 PHVHVQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILYYMRQMAVITPYAQFLFKFVSDTP 300
           PH+HVQEKRDNKDLWHGAEIQVVIEGNWTTYRSKIL+YMRQMAVITPYAQFLFKFVSDTP
Sbjct: 241 PHIHVQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300

Query: 301 DKNITIRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKS 360
           DKNI IRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKS
Sbjct: 301 DKNIAIRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKS 360

Query: 361 YAERLIGEMGPEFCPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420
           YAERLIGEMGPEF PKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII
Sbjct: 361 YAERLIGEMGPEFFPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420

Query: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480
           KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV
Sbjct: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480

Query: 481 TRTALKRINWSSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540
           TRTALKRINWSSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ
Sbjct: 481 TRTALKRINWSSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540

Query: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAE 600
           CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAE
Sbjct: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAE 600

Query: 601 ILSKVSARVITNETLRDKLAQHVEQVDHEMAMEYATQSGASAESKEEIYIQSLDATDNFT 660
           ILSKVSARVITNETLRDKLAQHVEQVDHEMAMEYATQSGASAESKEEIYIQSLDATDNFT
Sbjct: 601 ILSKVSARVITNETLRDKLAQHVEQVDHEMAMEYATQSGASAESKEEIYIQSLDATDNFT 660

Query: 661 DFKSPLFVFRLVH 674
           DFKSPLFVFRLVH
Sbjct: 661 DFKSPLFVFRLVH 673

BLAST of CmoCh02G012590.1 vs. ExPASy TrEMBL
Match: A0A5A7TIV7 (DNA topoisomerase 6 subunit B OS=Cucumis melo var. makuwa OX=1194695 GN=TOP6B PE=3 SV=1)

HSP 1 Score: 1273.8 bits (3295), Expect = 0.0e+00
Identity = 647/672 (96.28%), Postives = 664/672 (98.81%), Query Frame = 0

Query: 1   MEIGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKSLYTTVRE 60
           ME GGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGK LYTTVRE
Sbjct: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60

Query: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDAELYDDYETAKAREKRL 120
           LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVD ELYDDYETAKAREKRL
Sbjct: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120

Query: 121 AKEARAQEIQAKNASLGKKGREPAASKGMKGRGEASFYRVTCRDNGRGMPHDDIPNMFGR 180
           AKEARAQEIQAKNA+LGKKGREPAASKGMKGRGEASFYRVTC+DNG+GMPHDDIPNMFGR
Sbjct: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR 180

Query: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240
           VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI
Sbjct: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240

Query: 241 PHVHVQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILYYMRQMAVITPYAQFLFKFVSDTP 300
           PH+H+QEKRDNKDLWHGAEIQVVIEGNWTTYRSKIL+YMRQMAVITPYAQFLFKFVSDTP
Sbjct: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300

Query: 301 DKNITIRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKS 360
           DKNITIRFSRRTDVMPPVP ETKHHPSAVDLLLIKRLIAETAKQ+LLQFLQHEFVN+GKS
Sbjct: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS 360

Query: 361 YAERLIGEMGPEFCPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420
           +AERLIGEMGPEF PKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII
Sbjct: 361 FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420

Query: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480
           KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV
Sbjct: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480

Query: 481 TRTALKRINWSSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540
           TRTALKRINW+SYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ
Sbjct: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540

Query: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAE 600
           CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNA+YNVLKEMA VHASKKKRHD E+AE
Sbjct: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAIYNVLKEMAQVHASKKKRHDCEDAE 600

Query: 601 ILSKVSARVITNETLRDKLAQHVEQVDHEMAMEYATQSGASAESKEEIYIQSLDATDNFT 660
           ILSKVSAR+ITNETLRDKLA+HVEQVDHEMAMEYATQSGASAE KEE+YIQSLDATDNFT
Sbjct: 601 ILSKVSARLITNETLRDKLAEHVEQVDHEMAMEYATQSGASAEPKEEMYIQSLDATDNFT 660

Query: 661 DFKSPLFVFRLV 673
           DF+SPLFVFRL+
Sbjct: 661 DFQSPLFVFRLI 672

BLAST of CmoCh02G012590.1 vs. ExPASy TrEMBL
Match: A0A1S3BM58 (DNA topoisomerase 6 subunit B OS=Cucumis melo OX=3656 GN=LOC103491545 PE=3 SV=1)

HSP 1 Score: 1273.8 bits (3295), Expect = 0.0e+00
Identity = 647/672 (96.28%), Postives = 664/672 (98.81%), Query Frame = 0

Query: 1   MEIGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKSLYTTVRE 60
           ME GGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGK LYTTVRE
Sbjct: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60

Query: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDAELYDDYETAKAREKRL 120
           LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVD ELYDDYETAKAREKRL
Sbjct: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120

Query: 121 AKEARAQEIQAKNASLGKKGREPAASKGMKGRGEASFYRVTCRDNGRGMPHDDIPNMFGR 180
           AKEARAQEIQAKNA+LGKKGREPAASKGMKGRGEASFYRVTC+DNG+GMPHDDIPNMFGR
Sbjct: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR 180

Query: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240
           VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI
Sbjct: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240

Query: 241 PHVHVQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILYYMRQMAVITPYAQFLFKFVSDTP 300
           PH+H+QEKRDNKDLWHGAEIQVVIEGNWTTYRSKIL+YMRQMAVITPYAQFLFKFVSDTP
Sbjct: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300

Query: 301 DKNITIRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKS 360
           DKNITIRFSRRTDVMPPVP ETKHHPSAVDLLLIKRLIAETAKQ+LLQFLQHEFVN+GKS
Sbjct: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS 360

Query: 361 YAERLIGEMGPEFCPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420
           +AERLIGEMGPEF PKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII
Sbjct: 361 FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420

Query: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480
           KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV
Sbjct: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480

Query: 481 TRTALKRINWSSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540
           TRTALKRINW+SYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ
Sbjct: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540

Query: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAE 600
           CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNA+YNVLKEMA VHASKKKRHD E+AE
Sbjct: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAIYNVLKEMAQVHASKKKRHDCEDAE 600

Query: 601 ILSKVSARVITNETLRDKLAQHVEQVDHEMAMEYATQSGASAESKEEIYIQSLDATDNFT 660
           ILSKVSAR+ITNETLRDKLA+HVEQVDHEMAMEYATQSGASAE KEE+YIQSLDATDNFT
Sbjct: 601 ILSKVSARLITNETLRDKLAEHVEQVDHEMAMEYATQSGASAEPKEEMYIQSLDATDNFT 660

Query: 661 DFKSPLFVFRLV 673
           DF+SPLFVFRL+
Sbjct: 661 DFQSPLFVFRLI 672

BLAST of CmoCh02G012590.1 vs. ExPASy TrEMBL
Match: A0A6J1DJZ7 (DNA topoisomerase 6 subunit B OS=Momordica charantia OX=3673 GN=LOC111021237 PE=3 SV=1)

HSP 1 Score: 1261.1 bits (3262), Expect = 0.0e+00
Identity = 640/673 (95.10%), Postives = 661/673 (98.22%), Query Frame = 0

Query: 1   MEIGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKSLYTTVRE 60
           ME GGGSESPDEPKKGKSKTP+KPKESILKQKSPAEFFAENKNIAGFDNPGK LYTTVRE
Sbjct: 1   MEAGGGSESPDEPKKGKSKTPKKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60

Query: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDAELYDDYETAKAREKRL 120
           LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRER+DA LYDDYETAKAREKRL
Sbjct: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERIDAALYDDYETAKAREKRL 120

Query: 121 AKEARAQEIQAKNASLGKKGREPAASKGMKGRGEASFYRVTCRDNGRGMPHDDIPNMFGR 180
           AKEARAQEIQAKNA+LGKK REPAASKGMKGRGEASFYRVTC+DNGRGMPHDDIPNMFGR
Sbjct: 121 AKEARAQEIQAKNAALGKKVREPAASKGMKGRGEASFYRVTCKDNGRGMPHDDIPNMFGR 180

Query: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240
           VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNY SFCRLDIDIHRNI
Sbjct: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYISFCRLDIDIHRNI 240

Query: 241 PHVHVQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILYYMRQMAVITPYAQFLFKFVSDTP 300
           PH+H+QEK DNKDLWHGAEIQVVIEGNWTTYRSKIL+YMRQMAVITPYAQF+FKFVSDTP
Sbjct: 241 PHIHIQEKCDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFIFKFVSDTP 300

Query: 301 DKNITIRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKS 360
           DKNITIRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAET KQ+LLQFLQHEFVN+GKS
Sbjct: 301 DKNITIRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAETPKQNLLQFLQHEFVNIGKS 360

Query: 361 YAERLIGEMGPEFCPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420
           YAERLIGEMGPEF PKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGI+
Sbjct: 361 YAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIV 420

Query: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480
           KEL P+MVATYSGSAQVFEGHPFIVEAGVS+GGKDVKQGLNIFRFANRIPLLFEQGADVV
Sbjct: 421 KELQPEMVATYSGSAQVFEGHPFIVEAGVSVGGKDVKQGLNIFRFANRIPLLFEQGADVV 480

Query: 481 TRTALKRINWSSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540
           TRTALKRINWSSYK+NQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ
Sbjct: 481 TRTALKRINWSSYKMNQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540

Query: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAE 600
           CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMA VHASKKKRH+ E+AE
Sbjct: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQVHASKKKRHESEDAE 600

Query: 601 ILSKVSARVITNETLRDKLAQHVEQVDHEMAMEYATQSGASAESKEEIYIQSLDATDNFT 660
           ILSKVSAR+ITNETLRDKLAQHVEQVDHEMAMEYATQSGASAE +EEIYIQSL+ATDNFT
Sbjct: 601 ILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYATQSGASAEPREEIYIQSLEATDNFT 660

Query: 661 DFKSPLFVFRLVH 674
           DF+SPLFVFRLV+
Sbjct: 661 DFQSPLFVFRLVY 673

BLAST of CmoCh02G012590.1 vs. NCBI nr
Match: XP_022958156.1 (DNA topoisomerase 6 subunit B isoform X1 [Cucurbita moschata])

HSP 1 Score: 1316.2 bits (3405), Expect = 0.0e+00
Identity = 673/673 (100.00%), Postives = 673/673 (100.00%), Query Frame = 0

Query: 1   MEIGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKSLYTTVRE 60
           MEIGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKSLYTTVRE
Sbjct: 1   MEIGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKSLYTTVRE 60

Query: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDAELYDDYETAKAREKRL 120
           LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDAELYDDYETAKAREKRL
Sbjct: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDAELYDDYETAKAREKRL 120

Query: 121 AKEARAQEIQAKNASLGKKGREPAASKGMKGRGEASFYRVTCRDNGRGMPHDDIPNMFGR 180
           AKEARAQEIQAKNASLGKKGREPAASKGMKGRGEASFYRVTCRDNGRGMPHDDIPNMFGR
Sbjct: 121 AKEARAQEIQAKNASLGKKGREPAASKGMKGRGEASFYRVTCRDNGRGMPHDDIPNMFGR 180

Query: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240
           VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI
Sbjct: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240

Query: 241 PHVHVQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILYYMRQMAVITPYAQFLFKFVSDTP 300
           PHVHVQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILYYMRQMAVITPYAQFLFKFVSDTP
Sbjct: 241 PHVHVQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILYYMRQMAVITPYAQFLFKFVSDTP 300

Query: 301 DKNITIRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKS 360
           DKNITIRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKS
Sbjct: 301 DKNITIRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKS 360

Query: 361 YAERLIGEMGPEFCPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420
           YAERLIGEMGPEFCPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII
Sbjct: 361 YAERLIGEMGPEFCPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420

Query: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480
           KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV
Sbjct: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480

Query: 481 TRTALKRINWSSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540
           TRTALKRINWSSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ
Sbjct: 481 TRTALKRINWSSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540

Query: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAE 600
           CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAE
Sbjct: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAE 600

Query: 601 ILSKVSARVITNETLRDKLAQHVEQVDHEMAMEYATQSGASAESKEEIYIQSLDATDNFT 660
           ILSKVSARVITNETLRDKLAQHVEQVDHEMAMEYATQSGASAESKEEIYIQSLDATDNFT
Sbjct: 601 ILSKVSARVITNETLRDKLAQHVEQVDHEMAMEYATQSGASAESKEEIYIQSLDATDNFT 660

Query: 661 DFKSPLFVFRLVH 674
           DFKSPLFVFRLVH
Sbjct: 661 DFKSPLFVFRLVH 673

BLAST of CmoCh02G012590.1 vs. NCBI nr
Match: KAG7035834.1 (DNA topoisomerase 6 subunit B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1315.8 bits (3404), Expect = 0.0e+00
Identity = 672/673 (99.85%), Postives = 673/673 (100.00%), Query Frame = 0

Query: 1   MEIGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKSLYTTVRE 60
           MEIGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKSLYTTVRE
Sbjct: 1   MEIGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKSLYTTVRE 60

Query: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDAELYDDYETAKAREKRL 120
           LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDAELYDDYETAKAREKRL
Sbjct: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDAELYDDYETAKAREKRL 120

Query: 121 AKEARAQEIQAKNASLGKKGREPAASKGMKGRGEASFYRVTCRDNGRGMPHDDIPNMFGR 180
           AKEARAQEIQAKNASLGKKGREPAASKGMKGRGEASFYRVTCRDNGRGMPHDDIPNMFGR
Sbjct: 121 AKEARAQEIQAKNASLGKKGREPAASKGMKGRGEASFYRVTCRDNGRGMPHDDIPNMFGR 180

Query: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240
           VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI
Sbjct: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240

Query: 241 PHVHVQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILYYMRQMAVITPYAQFLFKFVSDTP 300
           PH+HVQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILYYMRQMAVITPYAQFLFKFVSDTP
Sbjct: 241 PHIHVQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILYYMRQMAVITPYAQFLFKFVSDTP 300

Query: 301 DKNITIRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKS 360
           DKNITIRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKS
Sbjct: 301 DKNITIRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKS 360

Query: 361 YAERLIGEMGPEFCPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420
           YAERLIGEMGPEFCPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII
Sbjct: 361 YAERLIGEMGPEFCPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420

Query: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480
           KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV
Sbjct: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480

Query: 481 TRTALKRINWSSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540
           TRTALKRINWSSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ
Sbjct: 481 TRTALKRINWSSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540

Query: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAE 600
           CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAE
Sbjct: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAE 600

Query: 601 ILSKVSARVITNETLRDKLAQHVEQVDHEMAMEYATQSGASAESKEEIYIQSLDATDNFT 660
           ILSKVSARVITNETLRDKLAQHVEQVDHEMAMEYATQSGASAESKEEIYIQSLDATDNFT
Sbjct: 601 ILSKVSARVITNETLRDKLAQHVEQVDHEMAMEYATQSGASAESKEEIYIQSLDATDNFT 660

Query: 661 DFKSPLFVFRLVH 674
           DFKSPLFVFRLVH
Sbjct: 661 DFKSPLFVFRLVH 673

BLAST of CmoCh02G012590.1 vs. NCBI nr
Match: KAG6605890.1 (DNA topoisomerase 6 subunit B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1313.5 bits (3398), Expect = 0.0e+00
Identity = 671/673 (99.70%), Postives = 672/673 (99.85%), Query Frame = 0

Query: 1   MEIGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKSLYTTVRE 60
           MEIGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKSLYTTVRE
Sbjct: 1   MEIGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKSLYTTVRE 60

Query: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDAELYDDYETAKAREKRL 120
           LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDAELYDDYETAKAREKRL
Sbjct: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDAELYDDYETAKAREKRL 120

Query: 121 AKEARAQEIQAKNASLGKKGREPAASKGMKGRGEASFYRVTCRDNGRGMPHDDIPNMFGR 180
           AKEARAQEIQAKNASLGKKGREPAASKGMKGRGEASFYRVTC+DNGRGMPHDDIPNMFGR
Sbjct: 121 AKEARAQEIQAKNASLGKKGREPAASKGMKGRGEASFYRVTCKDNGRGMPHDDIPNMFGR 180

Query: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240
           VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI
Sbjct: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240

Query: 241 PHVHVQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILYYMRQMAVITPYAQFLFKFVSDTP 300
           PHVHVQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILYYMRQMAVITPYAQFLFKFVSDTP
Sbjct: 241 PHVHVQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILYYMRQMAVITPYAQFLFKFVSDTP 300

Query: 301 DKNITIRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKS 360
           DKNITIRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKS
Sbjct: 301 DKNITIRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKS 360

Query: 361 YAERLIGEMGPEFCPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420
           YAERLIGEMGPEFCPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII
Sbjct: 361 YAERLIGEMGPEFCPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420

Query: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480
           KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV
Sbjct: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480

Query: 481 TRTALKRINWSSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540
           TRTALKRINWSSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ
Sbjct: 481 TRTALKRINWSSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540

Query: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAE 600
           CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAE
Sbjct: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAE 600

Query: 601 ILSKVSARVITNETLRDKLAQHVEQVDHEMAMEYATQSGASAESKEEIYIQSLDATDNFT 660
           ILSKVSAR ITNETLRDKLAQHVEQVDHEMAMEYATQSGASAESKEEIYIQSLDATDNFT
Sbjct: 601 ILSKVSARAITNETLRDKLAQHVEQVDHEMAMEYATQSGASAESKEEIYIQSLDATDNFT 660

Query: 661 DFKSPLFVFRLVH 674
           DFKSPLFVFRLVH
Sbjct: 661 DFKSPLFVFRLVH 673

BLAST of CmoCh02G012590.1 vs. NCBI nr
Match: XP_023532908.1 (DNA topoisomerase 6 subunit B isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1312.7 bits (3396), Expect = 0.0e+00
Identity = 670/673 (99.55%), Postives = 673/673 (100.00%), Query Frame = 0

Query: 1   MEIGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKSLYTTVRE 60
           MEIGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKSLYTTVRE
Sbjct: 1   MEIGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKSLYTTVRE 60

Query: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDAELYDDYETAKAREKRL 120
           LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDAELYDDYETAKAREKRL
Sbjct: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDAELYDDYETAKAREKRL 120

Query: 121 AKEARAQEIQAKNASLGKKGREPAASKGMKGRGEASFYRVTCRDNGRGMPHDDIPNMFGR 180
           AKEARAQEIQAKNASLGKKGREPAASKGMKGRGEASFYRVTC+DNGRGMPHDDIPNMFGR
Sbjct: 121 AKEARAQEIQAKNASLGKKGREPAASKGMKGRGEASFYRVTCKDNGRGMPHDDIPNMFGR 180

Query: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240
           VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI
Sbjct: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240

Query: 241 PHVHVQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILYYMRQMAVITPYAQFLFKFVSDTP 300
           PH+HVQEKRDNKDLWHGAEIQVVIEGNWTTYRSKIL+YMRQMAVITPYAQFLFKFVSDTP
Sbjct: 241 PHIHVQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300

Query: 301 DKNITIRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKS 360
           DKNITIRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKS
Sbjct: 301 DKNITIRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKS 360

Query: 361 YAERLIGEMGPEFCPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420
           YAERLIGEMGPEFCPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII
Sbjct: 361 YAERLIGEMGPEFCPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420

Query: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480
           KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV
Sbjct: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480

Query: 481 TRTALKRINWSSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540
           TRTALKRINWSSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ
Sbjct: 481 TRTALKRINWSSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540

Query: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAE 600
           CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAE
Sbjct: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAE 600

Query: 601 ILSKVSARVITNETLRDKLAQHVEQVDHEMAMEYATQSGASAESKEEIYIQSLDATDNFT 660
           ILSKVSARVITNETLRDKLAQHVEQVDHEMAMEYATQSGASAESKEEIYIQSLDATDNFT
Sbjct: 601 ILSKVSARVITNETLRDKLAQHVEQVDHEMAMEYATQSGASAESKEEIYIQSLDATDNFT 660

Query: 661 DFKSPLFVFRLVH 674
           DFKSPLFVFRLVH
Sbjct: 661 DFKSPLFVFRLVH 673

BLAST of CmoCh02G012590.1 vs. NCBI nr
Match: XP_022995035.1 (DNA topoisomerase 6 subunit B isoform X1 [Cucurbita maxima] >XP_022995036.1 DNA topoisomerase 6 subunit B isoform X1 [Cucurbita maxima])

HSP 1 Score: 1294.3 bits (3348), Expect = 0.0e+00
Identity = 662/673 (98.37%), Postives = 666/673 (98.96%), Query Frame = 0

Query: 1   MEIGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKSLYTTVRE 60
           MEIGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGK LYTTVRE
Sbjct: 1   MEIGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60

Query: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDAELYDDYETAKAREKRL 120
           LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVD ELYDDYETAKAREKRL
Sbjct: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120

Query: 121 AKEARAQEIQAKNASLGKKGREPAASKGMKGRGEASFYRVTCRDNGRGMPHDDIPNMFGR 180
            KEARAQEI AKNASLGKKGREPA SKGMKGRGEASFYRVTC+DNGRGMPHDDIPNMFGR
Sbjct: 121 VKEARAQEILAKNASLGKKGREPAVSKGMKGRGEASFYRVTCKDNGRGMPHDDIPNMFGR 180

Query: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240
           VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDI+RNI
Sbjct: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDINRNI 240

Query: 241 PHVHVQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILYYMRQMAVITPYAQFLFKFVSDTP 300
           PH+HVQEKRDNKDLWHGAEIQVVIEGNWTTYRSKIL+YMRQMAVITPYAQFLFKFVSDTP
Sbjct: 241 PHIHVQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300

Query: 301 DKNITIRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKS 360
           DKNI IRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKS
Sbjct: 301 DKNIAIRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKS 360

Query: 361 YAERLIGEMGPEFCPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420
           YAERLIGEMGPEF PKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII
Sbjct: 361 YAERLIGEMGPEFFPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420

Query: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480
           KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV
Sbjct: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480

Query: 481 TRTALKRINWSSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540
           TRTALKRINWSSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ
Sbjct: 481 TRTALKRINWSSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540

Query: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAE 600
           CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAE
Sbjct: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAE 600

Query: 601 ILSKVSARVITNETLRDKLAQHVEQVDHEMAMEYATQSGASAESKEEIYIQSLDATDNFT 660
           ILSKVSARVITNETLRDKLAQHVEQVDHEMAMEYATQSGASAESKEEIYIQSLDATDNFT
Sbjct: 601 ILSKVSARVITNETLRDKLAQHVEQVDHEMAMEYATQSGASAESKEEIYIQSLDATDNFT 660

Query: 661 DFKSPLFVFRLVH 674
           DFKSPLFVFRLVH
Sbjct: 661 DFKSPLFVFRLVH 673

BLAST of CmoCh02G012590.1 vs. TAIR 10
Match: AT3G20780.1 (topoisomerase 6 subunit B )

HSP 1 Score: 1082.0 bits (2797), Expect = 0.0e+00
Identity = 540/662 (81.57%), Postives = 604/662 (91.24%), Query Frame = 0

Query: 12  EPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKSLYTTVRELVENSLDSAES 71
           E KKG SK  +   ES LKQKSPAEFFAENKNIAGFDNPGKSLYTTVRELVEN+LDSAES
Sbjct: 8   ETKKGSSKNSKDSNESKLKQKSPAEFFAENKNIAGFDNPGKSLYTTVRELVENALDSAES 67

Query: 72  ISELPVVEITIEEILKTKFNSMIGLVDRERVDAELYDDYETAKAREKRLAKEARAQEIQA 131
           ISELP VE+TIEEI+K+KFNSMIGL+DRERVD +LYDDYET KAR KRLAKEARA EIQA
Sbjct: 68  ISELPEVEVTIEEIVKSKFNSMIGLIDRERVDTQLYDDYETEKARGKRLAKEARASEIQA 127

Query: 132 KNASLGKKGREPAASKGMKGRGEASFYRVTCRDNGRGMPHDDIPNMFGRVLSGTKYGLKQ 191
           KN + GKK +EP  SK +K RGEAS+Y+VTC+DNG+GMPHDDIPNMFGRVLSGTKYGLKQ
Sbjct: 128 KNLASGKKNKEPGVSKVLKARGEASYYKVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQ 187

Query: 192 TRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNIPHVHVQEKRDN 251
           TRGKFGLGAKMALIWSKMSTGLPIEISSSMK QNY +FCRLDIDIHRNIPH+H+ EK+ N
Sbjct: 188 TRGKFGLGAKMALIWSKMSTGLPIEISSSMKSQNYVTFCRLDIDIHRNIPHIHLHEKKGN 247

Query: 252 KDLWHGAEIQVVIEGNWTTYRSKILYYMRQMAVITPYAQFLFKFVSDTPDKNITIRFSRR 311
           K+ WHGAEIQVVIEGNWTTYRSKIL+YMRQMAVITPYAQFLF+F+S+TP+KN+TI+F+RR
Sbjct: 248 KEKWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFRFISETPEKNVTIKFTRR 307

Query: 312 TDVMPPVPQETKHHPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKSYAERLIGEMGP 371
           TDVMPP+P ETKHHPS+VDLLLIKRLI +T+K++LLQFLQ+EFVN+ K+ A RLIGEMGP
Sbjct: 308 TDVMPPIPIETKHHPSSVDLLLIKRLITDTSKKTLLQFLQNEFVNINKTLAARLIGEMGP 367

Query: 372 EFCPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATY 431
           +F P M+VKS+TSQQ+VRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATY
Sbjct: 368 DFGPSMAVKSVTSQQMVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATY 427

Query: 432 SGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWS 491
           SGSAQVFEGHPFIVEAGVS+GG+DVKQG+NIFRFANRIPLLFEQGADVVTRTALKRINW+
Sbjct: 428 SGSAQVFEGHPFIVEAGVSLGGRDVKQGINIFRFANRIPLLFEQGADVVTRTALKRINWN 487

Query: 492 SYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQCCIQLKSKIVK 551
           SYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIA+AVKSAIQQCCIQLKSKIVK
Sbjct: 488 SYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIATAVKSAIQQCCIQLKSKIVK 547

Query: 552 KIQAREQQERKRNLSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAEILSKVSARVIT 611
           ++QAREQQERKR+LSRYIPDAT AVY VLK+M   H +K+KR+  E+  +L KVS ++IT
Sbjct: 548 RLQAREQQERKRSLSRYIPDATGAVYEVLKQMTEEHKTKRKRYGEEDIVMLDKVSKQIIT 607

Query: 612 NETLRDKLAQHVEQVDHEMAMEYATQSGASAESKEEIYIQSLDAT-DNFTDFKSPLFVFR 671
            ETL++KLA+HVEQVD+EMA+EYATQSG S E +E IY+Q LD    NF D  SP FVFR
Sbjct: 608 KETLKEKLAEHVEQVDYEMALEYATQSGVSEEPRENIYLQHLDPNKSNFIDLHSPTFVFR 667

Query: 672 LV 673
           L+
Sbjct: 668 LM 669