CmoCh02G010330.1 (mRNA) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh02G010330.1
Type	mRNA
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	NAD(P)-binding Rossmann-fold superfamily protein
Location	Cmo_Chr02: 6278550 .. 6279816 (+)
Sequence length	1190
RNA-Seq Expression	CmoCh02G010330.1
Synteny	CmoCh02G010330.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTAAAAGTACCTTTTCCCCATTCCCTTCCTAAGTTATTCAAATGGGATCACTCTGAATCCGCCATTGAAGCCCCATCCGAGCTCCTCATTCAAACCTTCTGAAACGCCATGATTCGGAGTCATCTCGTAAGGAATTCGCTTACTTTGAGCTTCTTCAATAGGGGATTGTCTTCCCATGCTGGAAGGTGAGTTTGTTTATTATACTCTCTTCCAAAACCTTCAATGTTCTTGACATGGGTTAGATTTGGATTGGGATATGCAGGCTTGAAGGCAAGATTGCGCTGATTACAGGCGCAGCCAGCGGCATTGGGAAGGCAACGGCATCCAAATTCATAAGCAATGGAGCCAAAGTTGTGTTGGCGGATGTCGAGGAAGAGCTTGGCCAAGCGACGGCCAAGCAGCTCGGCCCCAACGCTACCTTCATCCCTTGTGATGTCAGCAACGAGGCCAATGTCTCTGATGCTGTAGATTTCGCTCTCTCTCTTCACAGAAGGCTTGACATAATGTACAACAATGCTGGGATTGCTTGCAATACTCCTCCAAGCATTTTGGACCTCGACCTTGCCATATTCGATAAAGTTATGAACATTAATGTCCGAGGTGTTGTAGCTGGTATCAAGCACGCCTCTCGTGTTATGATCCCTCAACAATCAGGATCAATTTTATGCACGGCTAGTGTCACGGGAGTGATGGGTGGGTTAGCTCAACATACGTATTCTGTGTCGAAGGTCGCTGTTATCGGTATTGTCAAGTCCATGGCATCGGAGTTGTGTAAATATGGTATTCGAGTGAATTGCATATCACCATTTGCGATCCCGACGCCATTTTCTATCAACGAAATGTGTCGCTTTTTTCCTGGAACTGAGAAGGAGAAACTTGAGAAGATGATATTTGATTTGAGTGCTTTGGAAGGGGCAGTTTGTGAGACTAATGATATAGCCAATGCTGCACTTTATCTTGCATCTGATGATGCAAAGTATGTAAGTGGGCACAATTTGGTGGTTGATGGCAGCTTTACCTGTTTCAAGAGGTTAAACTTTCCTCTCCCTGATCAGAATCCATCATGATTCTGTATATCCTGTTGTTTTTGCTCATTTATATTAGAAGAAAGAGCTCAAATTACACATAATTATGTTAGAGCCTTGAAAGGGTTGTTCTAGGACCTTCAAAGTTGTCCCGAAAAATGCTCTTAAAGAGTCGAATCGATTCTATGTCGTACTTTTTGTCTCATGTATTATGTCAAGTAATAGGAGGCTTCATTCAAAAC

mRNA sequence

TTAAAAGTACCTTTTCCCCATTCCCTTCCTAAGTTATTCAAATGGGATCACTCTGAATCCGCCATTGAAGCCCCATCCGAGCTCCTCATTCAAACCTTCTGAAACGCCATGATTCGGAGTCATCTCGTAAGGAATTCGCTTACTTTGAGCTTCTTCAATAGGGGATTGTCTTCCCATGCTGGAAGGCTTGAAGGCAAGATTGCGCTGATTACAGGCGCAGCCAGCGGCATTGGGAAGGCAACGGCATCCAAATTCATAAGCAATGGAGCCAAAGTTGTGTTGGCGGATGTCGAGGAAGAGCTTGGCCAAGCGACGGCCAAGCAGCTCGGCCCCAACGCTACCTTCATCCCTTGTGATGTCAGCAACGAGGCCAATGTCTCTGATGCTGTAGATTTCGCTCTCTCTCTTCACAGAAGGCTTGACATAATGTACAACAATGCTGGGATTGCTTGCAATACTCCTCCAAGCATTTTGGACCTCGACCTTGCCATATTCGATAAAGTTATGAACATTAATGTCCGAGGTGTTGTAGCTGGTATCAAGCACGCCTCTCGTGTTATGATCCCTCAACAATCAGGATCAATTTTATGCACGGCTAGTGTCACGGGAGTGATGGGTGGGTTAGCTCAACATACGTATTCTGTGTCGAAGGTCGCTGTTATCGGTATTGTCAAGTCCATGGCATCGGAGTTGTGTAAATATGGTATTCGAGTGAATTGCATATCACCATTTGCGATCCCGACGCCATTTTCTATCAACGAAATGTGTCGCTTTTTTCCTGGAACTGAGAAGGAGAAACTTGAGAAGATGATATTTGATTTGAGTGCTTTGGAAGGGGCAGTTTGTGAGACTAATGATATAGCCAATGCTGCACTTTATCTTGCATCTGATGATGCAAAGTATGTAAGTGGGCACAATTTGGTGGTTGATGGCAGCTTTACCTGTTTCAAGAGGTTAAACTTTCCTCTCCCTGATCAGAATCCATCATGATTCTGTATATCCTGTTGTTTTTGCTCATTTATATTAGAAGAAAGAGCTCAAATTACACATAATTATGTTAGAGCCTTGAAAGGGTTGTTCTAGGACCTTCAAAGTTGTCCCGAAAAATGCTCTTAAAGAGTCGAATCGATTCTATGTCGTACTTTTTGTCTCATGTATTATGTCAAGTAATAGGAGGCTTCATTCAAAAC

Coding sequence (CDS)

ATGATTCGGAGTCATCTCGTAAGGAATTCGCTTACTTTGAGCTTCTTCAATAGGGGATTGTCTTCCCATGCTGGAAGGCTTGAAGGCAAGATTGCGCTGATTACAGGCGCAGCCAGCGGCATTGGGAAGGCAACGGCATCCAAATTCATAAGCAATGGAGCCAAAGTTGTGTTGGCGGATGTCGAGGAAGAGCTTGGCCAAGCGACGGCCAAGCAGCTCGGCCCCAACGCTACCTTCATCCCTTGTGATGTCAGCAACGAGGCCAATGTCTCTGATGCTGTAGATTTCGCTCTCTCTCTTCACAGAAGGCTTGACATAATGTACAACAATGCTGGGATTGCTTGCAATACTCCTCCAAGCATTTTGGACCTCGACCTTGCCATATTCGATAAAGTTATGAACATTAATGTCCGAGGTGTTGTAGCTGGTATCAAGCACGCCTCTCGTGTTATGATCCCTCAACAATCAGGATCAATTTTATGCACGGCTAGTGTCACGGGAGTGATGGGTGGGTTAGCTCAACATACGTATTCTGTGTCGAAGGTCGCTGTTATCGGTATTGTCAAGTCCATGGCATCGGAGTTGTGTAAATATGGTATTCGAGTGAATTGCATATCACCATTTGCGATCCCGACGCCATTTTCTATCAACGAAATGTGTCGCTTTTTTCCTGGAACTGAGAAGGAGAAACTTGAGAAGATGATATTTGATTTGAGTGCTTTGGAAGGGGCAGTTTGTGAGACTAATGATATAGCCAATGCTGCACTTTATCTTGCATCTGATGATGCAAAGTATGTAAGTGGGCACAATTTGGTGGTTGATGGCAGCTTTACCTGTTTCAAGAGGTTAAACTTTCCTCTCCCTGATCAGAATCCATCATGA

Protein sequence

MIRSHLVRNSLTLSFFNRGLSSHAGRLEGKIALITGAASGIGKATASKFISNGAKVVLADVEEELGQATAKQLGPNATFIPCDVSNEANVSDAVDFALSLHRRLDIMYNNAGIACNTPPSILDLDLAIFDKVMNINVRGVVAGIKHASRVMIPQQSGSILCTASVTGVMGGLAQHTYSVSKVAVIGIVKSMASELCKYGIRVNCISPFAIPTPFSINEMCRFFPGTEKEKLEKMIFDLSALEGAVCETNDIANAALYLASDDAKYVSGHNLVVDGSFTCFKRLNFPLPDQNPS

Homology

[Close]

BLAST of CmoCh02G010330.1 vs. ExPASy Swiss-Prot
Match: Q94KL7 (Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 PE=1 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 4.1e-63
Identity = 130/261 (49.81%), Postives = 184/261 (70.50%), Query Frame = 0


Query: 20  LSSHAGRLEGKIALITGAASGIGKATASKFISNGAKVVLADVEEELGQATAKQLG-PNAT 79
           L++ A RLEGK+ALITG ASGIG+ TA  F  +GAKV +ADV++ELG +  + +G  N+T
Sbjct: 8   LTAIARRLEGKVALITGGASGIGETTAKLFSQHGAKVAIADVQDELGHSVVEAIGTSNST 67

Query: 80  FIPCDVSNEANVSDAVDFALSLHRRLDIMYNNAGIACNTPPSILDLDLAIFDKVMNINVR 139
           +I CDV+NE  V +AVD  +S + +LDIM++NAGI+    P I+D + A F++V ++NV 
Sbjct: 68  YIHCDVTNEDGVKNAVDNTVSTYGKLDIMFSNAGISDPNRPRIIDNEKADFERVFSVNVT 127

Query: 140 GVVAGIKHASRVMIPQQSGSILCTASVTGVMGGLAQHTYSVSKVAVIGIVKSMASELCKY 199
           GV   +KHA+RVMIP +SG+I+ TAS++  MGG + H Y  SK AV+G+ +++A EL ++
Sbjct: 128 GVFLCMKHAARVMIPARSGNIISTASLSSTMGGGSSHAYCGSKHAVLGLTRNLAVELGQF 187

Query: 200 GIRVNCISPFAIPTPFSINEMCRFFPGTE-KEKLEKMIFDLSALEGAVCETNDIANAALY 259
           GIRVNC+SPF +PT        + F G + +E+ E +I     L+G      D+ANAALY
Sbjct: 188 GIRVNCLSPFGLPTALG-----KKFSGIKNEEEFENVINFAGNLKGPKFNVEDVANAALY 247

Query: 260 LASDDAKYVSGHNLVVDGSFT 279
           LASD+AKYVSGHNL +DG F+
Sbjct: 248 LASDEAKYVSGHNLFIDGGFS 263

[Close]

BLAST of CmoCh02G010330.1 vs. ExPASy Swiss-Prot
Match: Q9SCU0 (Short-chain dehydrogenase reductase 2a OS=Arabidopsis thaliana OX=3702 GN=SDR2a PE=3 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 6.5e-61
Identity = 132/265 (49.81%), Postives = 172/265 (64.91%), Query Frame = 0


Query: 26  RLEGKIALITGAASGIGKATASKFISNGAKVVLADVEEELGQATAKQL-----GPNATFI 85
           RLEGK+A+ITG A GIGKAT   F  +GA VV+ADV+   G + AK L      P   FI
Sbjct: 31  RLEGKVAIITGGAHGIGKATVMLFARHGATVVIADVDNVAGSSLAKSLSSHKTSPMVAFI 90

Query: 86  PCDVSNEANVSDAVDFALSLHRRLDIMYNNAGIACNTP--PSILDLDLAIFDKVMNINVR 145
            CDVS EA+V + V+  ++ + RLDI++NNAG+  +     SILD D   FD VM +NVR
Sbjct: 91  SCDVSVEADVENLVNVTVARYGRLDILFNNAGVLGDQKKHKSILDFDADEFDHVMRVNVR 150

Query: 146 GVVAGIKHASRVMIPQQ-SGSILCTASVTGVMGGLAQHTYSVSKVAVIGIVKSMASELCK 205
           GV  G+KH +R MI +   G I+ TASV GVMGG+  H Y+ SK A++G+ K+ A EL K
Sbjct: 151 GVGLGMKHGARAMIKRGFKGCIISTASVAGVMGGMGPHAYTASKHAIVGLTKNAACELGK 210

Query: 206 YGIRVNCISPFAIPTPFSINEMCRFFPG----TEKEKLEKMIFDLSALEGAVCETNDIAN 265
           YGIRVNCISPF + T   +N   +   G     + E++E+ +  L+ L+G     NDIA 
Sbjct: 211 YGIRVNCISPFGVATSMLVNAWRKTSGGDVEDDDVEEMEEFVRSLANLKGETLRANDIAE 270

Query: 266 AALYLASDDAKYVSGHNLVVDGSFT 279
           AALYLASD++KYV+GHNLVVDG  T
Sbjct: 271 AALYLASDESKYVNGHNLVVDGGVT 295

[Close]

BLAST of CmoCh02G010330.1 vs. ExPASy Swiss-Prot
Match: P50160 (Sex determination protein tasselseed-2 OS=Zea mays OX=4577 GN=TS2 PE=2 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 1.2e-59
Identity = 125/282 (44.33%), Postives = 175/282 (62.06%), Query Frame = 0


Query: 26  RLEGKIALITGAASGIGKATASKFISNGAKVVLADVEEELGQATAKQLGPNATFIPCDVS 85
           RL+GK+A++TG A GIG+A    F  +GA+VV+AD+++  G+A A  LGP  +F+ CDVS
Sbjct: 52  RLDGKVAIVTGGARGIGEAIVRLFAKHGARVVIADIDDAAGEALASALGPQVSFVRCDVS 111

Query: 86  NEANVSDAVDFALSLH-RRLDIMYNNAGI---ACNTPPSILDLDLAIFDKVMNINVRGVV 145
            E +V  AVD+ALS H  RLD+  NNAG+         SIL  D A FD+V+ +N  G  
Sbjct: 112 VEDDVRRAVDWALSRHGGRLDVYCNNAGVLGRQTRAARSILSFDAAEFDRVLRVNALGAA 171

Query: 146 AGIKHASRVMIPQQSGSILCTASVTGVMGGLAQHTYSVSKVAVIGIVKSMASELCKYGIR 205
            G+KHA+R M P+++GSI+  ASV  V+GGL  H Y+ SK A++G+ K+ A EL  +G+R
Sbjct: 172 LGMKHAARAMAPRRAGSIVSVASVAAVLGGLGPHAYTASKHAIVGLTKNAACELRAHGVR 231

Query: 206 VNCISPFAIPTPFSIN-------------------EMCRFFPG-TEKEKLEKMIFDLSAL 265
           VNC+SPF + TP  IN                   ++    P   E EK+E+++  L+ L
Sbjct: 232 VNCVSPFGVATPMLINAWRQGHDDATADADRDLDLDLDVTVPSDQEVEKMEEVVRGLATL 291

Query: 266 EGAVCETNDIANAALYLASDDAKYVSGHNLVVDGSFTCFKRL 284
           +G      DIA A L+LASD+A+Y+SGHNLVVDG  T  + L
Sbjct: 292 KGPTLRPRDIAEAVLFLASDEARYISGHNLVVDGGVTTSRNL 333

[Close]

BLAST of CmoCh02G010330.1 vs. ExPASy Swiss-Prot
Match: H9BFQ0 (Tropinone reductase-like 1 OS=Erythroxylum coca OX=289672 PE=2 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 4.4e-57
Identity = 121/256 (47.27%), Postives = 171/256 (66.80%), Query Frame = 0


Query: 26  RLEGKIALITGAASGIGKATASKFISNGAKVVLADVEEELGQATAKQLGPNATFIPCDVS 85
           RLEGK+A+ITG ASGIG  TA  F  NGAKVV+AD++++LGQA A +LG  A +I CDVS
Sbjct: 10  RLEGKVAIITGGASGIGACTAELFHENGAKVVIADIQDDLGQALATKLGGKACYIHCDVS 69

Query: 86  NEANVSDAVDFALSLHRRLDIMYNNAGI--ACNTPPSILDLDLAIFDKVMNINVRGVVAG 145
            E +V + VD  ++ + RLDIM+NNAGI      P S+++ + +  D+++++N+ G   G
Sbjct: 70  KEDDVINLVDTTVAKYGRLDIMFNNAGIIEGQGLPVSVVESEKSDLDRLLSVNLGGAFLG 129

Query: 146 IKHASRVMIPQQSGSILCTASVTGVMGGLAQHTYSVSKVAVIGIVKSMASELCKYGIRVN 205
            KHA+RVM+ Q+ G IL T+S+   + GL+ H Y+ SK  V G+ K++  EL KYGIRVN
Sbjct: 130 AKHATRVMVQQRKGCILFTSSLCTSIAGLSGHAYAASKSGVCGLAKNLTPELGKYGIRVN 189

Query: 206 CISPFAIPTPFS-INEMCRFFPGTEKEKLEKMIFDLSALEGAVCETNDIANAALYLASDD 265
           CISP+ + T  S I+E         +E +E M+ +L  L G     + IA AAL+LASD+
Sbjct: 190 CISPYGLVTGISNISE-------ANRELVEAMLSELGTLSGQTLRADGIAKAALFLASDE 249

Query: 266 AKYVSGHNLVVDGSFT 279
           A YVSG N+VVDG ++
Sbjct: 250 AYYVSGINMVVDGGYS 258

[Close]

BLAST of CmoCh02G010330.1 vs. ExPASy Swiss-Prot
Match: F1SWA0 (Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 7.5e-57
Identity = 120/260 (46.15%), Postives = 173/260 (66.54%), Query Frame = 0


Query: 26  RLEGKIALITGAASGIGKATASKFISNGAKVVLADVEEELGQATAKQLG--PNATFIPCD 85
           RLEGK+AL+TG ASGIG++ A  FI +GAK+ + DV++ELGQ  +++LG  P+A +  CD
Sbjct: 2   RLEGKVALVTGGASGIGESIARLFIEHGAKICIVDVQDELGQQVSQRLGGDPHACYFHCD 61

Query: 86  VSNEANVSDAVDFALSLHRRLDIMYNNAGIACNTPPSILDLDLAIFDKVMNINVRGVVAG 145
           V+ E +V  AVDF    +  +DIM NNAGI  +    I D D   F KV +INV GV  G
Sbjct: 62  VTVEDDVRRAVDFTAEKYGTIDIMVNNAGITGDKVIDIRDADFNEFKKVFDINVNGVFLG 121

Query: 146 IKHASRVMIPQQSGSILCTASVTGVMGGLAQHTYSVSKVAVIGIVKSMASELCKYGIRVN 205
           +KHA+R+MIP+  GSI+  ASV+ V+ G   H Y+ +K AV+G+ KS+A+EL ++GIRVN
Sbjct: 122 MKHAARIMIPKMKGSIVSLASVSSVIAGAGPHGYTGAKHAVVGLTKSVAAELGRHGIRVN 181

Query: 206 CISPFAIPTPFSINEMCRFFPGTEKEK-----LEKMIFDLSALEGAVCETNDIANAALYL 265
           C+SP+A+PT  S+     + P +E ++         +   + L+G     ND+A A LYL
Sbjct: 182 CVSPYAVPTRLSM----PYLPESEMQEDALRGFLTFVRSNANLKGVDLMPNDVAEAVLYL 241

Query: 266 ASDDAKYVSGHNLVVDGSFT 279
           A++++KYVSG NLV+DG F+
Sbjct: 242 ATEESKYVSGLNLVIDGGFS 257

[Close]

BLAST of CmoCh02G010330.1 vs. ExPASy TrEMBL
Match: A0A6J1H354 (secoisolariciresinol dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111459685 PE=4 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 9.8e-161
Identity = 293/293 (100.00%), Postives = 293/293 (100.00%), Query Frame = 0


Query: 1   MIRSHLVRNSLTLSFFNRGLSSHAGRLEGKIALITGAASGIGKATASKFISNGAKVVLAD 60
           MIRSHLVRNSLTLSFFNRGLSSHAGRLEGKIALITGAASGIGKATASKFISNGAKVVLAD
Sbjct: 1   MIRSHLVRNSLTLSFFNRGLSSHAGRLEGKIALITGAASGIGKATASKFISNGAKVVLAD 60

Query: 61  VEEELGQATAKQLGPNATFIPCDVSNEANVSDAVDFALSLHRRLDIMYNNAGIACNTPPS 120
           VEEELGQATAKQLGPNATFIPCDVSNEANVSDAVDFALSLHRRLDIMYNNAGIACNTPPS
Sbjct: 61  VEEELGQATAKQLGPNATFIPCDVSNEANVSDAVDFALSLHRRLDIMYNNAGIACNTPPS 120

Query: 121 ILDLDLAIFDKVMNINVRGVVAGIKHASRVMIPQQSGSILCTASVTGVMGGLAQHTYSVS 180
           ILDLDLAIFDKVMNINVRGVVAGIKHASRVMIPQQSGSILCTASVTGVMGGLAQHTYSVS
Sbjct: 121 ILDLDLAIFDKVMNINVRGVVAGIKHASRVMIPQQSGSILCTASVTGVMGGLAQHTYSVS 180

Query: 181 KVAVIGIVKSMASELCKYGIRVNCISPFAIPTPFSINEMCRFFPGTEKEKLEKMIFDLSA 240
           KVAVIGIVKSMASELCKYGIRVNCISPFAIPTPFSINEMCRFFPGTEKEKLEKMIFDLSA
Sbjct: 181 KVAVIGIVKSMASELCKYGIRVNCISPFAIPTPFSINEMCRFFPGTEKEKLEKMIFDLSA 240

Query: 241 LEGAVCETNDIANAALYLASDDAKYVSGHNLVVDGSFTCFKRLNFPLPDQNPS 294
           LEGAVCETNDIANAALYLASDDAKYVSGHNLVVDGSFTCFKRLNFPLPDQNPS
Sbjct: 241 LEGAVCETNDIANAALYLASDDAKYVSGHNLVVDGSFTCFKRLNFPLPDQNPS 293

[Close]

BLAST of CmoCh02G010330.1 vs. ExPASy TrEMBL
Match: A0A6J1K592 (secoisolariciresinol dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111491301 PE=4 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 9.5e-156
Identity = 282/293 (96.25%), Postives = 289/293 (98.63%), Query Frame = 0


Query: 1   MIRSHLVRNSLTLSFFNRGLSSHAGRLEGKIALITGAASGIGKATASKFISNGAKVVLAD 60
           MIRSHL+RNSLTLSFFNRGLSSHAGRLEGKIALITGAASGIGKATASKFISNGAKVVLAD
Sbjct: 1   MIRSHLLRNSLTLSFFNRGLSSHAGRLEGKIALITGAASGIGKATASKFISNGAKVVLAD 60

Query: 61  VEEELGQATAKQLGPNATFIPCDVSNEANVSDAVDFALSLHRRLDIMYNNAGIACNTPPS 120
           VEEELGQATAKQLGPNATFIPCDVSNE+NVSDAVDFALSLH+RLDIM NNAGIACNTPPS
Sbjct: 61  VEEELGQATAKQLGPNATFIPCDVSNESNVSDAVDFALSLHKRLDIMCNNAGIACNTPPS 120

Query: 121 ILDLDLAIFDKVMNINVRGVVAGIKHASRVMIPQQSGSILCTASVTGVMGGLAQHTYSVS 180
           ILDLDLA+FDKVMNINVRGV+AGIKHASRVMIPQQSGSILCTASVTGVMGGLAQHTYSVS
Sbjct: 121 ILDLDLAVFDKVMNINVRGVLAGIKHASRVMIPQQSGSILCTASVTGVMGGLAQHTYSVS 180

Query: 181 KVAVIGIVKSMASELCKYGIRVNCISPFAIPTPFSINEMCRFFPGTEKEKLEKMIFDLSA 240
           KVAVIGIVKSMASELCKYGIRVNCISPFAIPTPF+INEMC  FPGTEKEKLEKMIFDLSA
Sbjct: 181 KVAVIGIVKSMASELCKYGIRVNCISPFAIPTPFAINEMCHLFPGTEKEKLEKMIFDLSA 240

Query: 241 LEGAVCETNDIANAALYLASDDAKYVSGHNLVVDGSFTCFKRLNFPLPDQNPS 294
           LEGAVCETNDIANAALYLASDDAKYVSGHNLVVDG FTCFKRLNFPLPDQNP+
Sbjct: 241 LEGAVCETNDIANAALYLASDDAKYVSGHNLVVDGGFTCFKRLNFPLPDQNPT 293

[Close]

BLAST of CmoCh02G010330.1 vs. ExPASy TrEMBL
Match: A0A6J1DLW0 (secoisolariciresinol dehydrogenase OS=Momordica charantia OX=3673 GN=LOC111021770 PE=4 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 1.5e-132
Identity = 236/281 (83.99%), Postives = 263/281 (93.59%), Query Frame = 0


Query: 9   NSLTLSFFNRGLSSHAGRLEGKIALITGAASGIGKATASKFISNGAKVVLADVEEELGQA 68
           NSLT SFFNR LSSHAGRLEGKIALITGAASGIGKATASKFI+NGAKVV+ADV+E+LG  
Sbjct: 22  NSLTQSFFNRELSSHAGRLEGKIALITGAASGIGKATASKFINNGAKVVIADVKEKLGLE 81

Query: 69  TAKQLGPNATFIPCDVSNEANVSDAVDFALSLHRRLDIMYNNAGIACNTPPSILDLDLAI 128
           TA+QLGPNAT++PCDV+ E++VSDAVD+ +SLH++LDIMYNNAG+ACNTPPSI+DLDLA+
Sbjct: 82  TAEQLGPNATYVPCDVTKESDVSDAVDYTISLHKKLDIMYNNAGVACNTPPSIVDLDLAV 141

Query: 129 FDKVMNINVRGVVAGIKHASRVMIPQQSGSILCTASVTGVMGGLAQHTYSVSKVAVIGIV 188
           FDKVMN+NVRGV+ GIKHASRVMIP+QSGSILCTASVTG+MGGLAQHTYSVSKVA+IG+V
Sbjct: 142 FDKVMNVNVRGVLVGIKHASRVMIPRQSGSILCTASVTGLMGGLAQHTYSVSKVAIIGMV 201

Query: 189 KSMASELCKYGIRVNCISPFAIPTPFSINEMCRFFPGTEKEKLEKMIFDLSALEGAVCET 248
           KSMASELCKYGIRVNCISPF IPTPF + EM R FP TE++KLEKMIFD  ALEG VCET
Sbjct: 202 KSMASELCKYGIRVNCISPFPIPTPFVMEEMSRLFPRTEEQKLEKMIFDTGALEGTVCET 261

Query: 249 NDIANAALYLASDDAKYVSGHNLVVDGSFTCFKRLNFPLPD 290
           NDIANAALYLASDDAKYVSGHNLVVDG FTCFK LNFP+PD
Sbjct: 262 NDIANAALYLASDDAKYVSGHNLVVDGGFTCFKCLNFPVPD 302

[Close]

BLAST of CmoCh02G010330.1 vs. ExPASy TrEMBL
Match: A0A1S3CT49 (secoisolariciresinol dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103504658 PE=4 SV=1)

HSP 1 Score: 481.5 bits (1238), Expect = 2.5e-132
Identity = 242/287 (84.32%), Postives = 263/287 (91.64%), Query Frame = 0


Query: 6   LVRNSLTLS-FFNRGLSSHAGRLEGKIALITGAASGIGKATASKFISNGAKVVLADVEEE 65
           L  NSLT S FFNR  SS  GRLEGKIALITGAASGIGKATA KFISNGAKVV+AD++E+
Sbjct: 10  LFNNSLTWSFFFNRRFSSQPGRLEGKIALITGAASGIGKATAGKFISNGAKVVIADIKEK 69

Query: 66  LGQATAKQLGPNATFIPCDVSNEANVSDAVDFALSLHRRLDIMYNNAGIACNTPPSILDL 125
           LGQ TAKQLGPNATFI CDV+ E+N+SDAVDF +SLH++LDIMYNNAGIACNTPPSI DL
Sbjct: 70  LGQDTAKQLGPNATFIHCDVTKESNISDAVDFTISLHKKLDIMYNNAGIACNTPPSISDL 129

Query: 126 DLAIFDKVMNINVRGVVAGIKHASRVMIPQQSGSILCTASVTGVMGGLAQHTYSVSKVAV 185
           DLA+FDKVMN+NVRGV+AGIKHASRVMIP++SGSILCTASVTG+MGGLAQHTYSVSKVAV
Sbjct: 130 DLAVFDKVMNVNVRGVLAGIKHASRVMIPRRSGSILCTASVTGLMGGLAQHTYSVSKVAV 189

Query: 186 IGIVKSMASELCKYGIRVNCISPFAIPTPFSINEMCRFFPGTEKEKLEKMIFDLSALEGA 245
           IGIVKS+ASELC+YGIRVNCISPF IPTPF I EM + FP  E+EKLEKMIFDLSALEG 
Sbjct: 190 IGIVKSLASELCRYGIRVNCISPFPIPTPFVIEEMTQLFPSVEEEKLEKMIFDLSALEGT 249

Query: 246 VCETNDIANAALYLASDDAKYVSGHNLVVDGSFTCFKRLNFPLPDQN 292
           VCETNDIANAALYLASDDAKYVSGHNLVVDG+FTCFK LNF LPDQN
Sbjct: 250 VCETNDIANAALYLASDDAKYVSGHNLVVDGAFTCFKSLNFSLPDQN 296

[Close]

BLAST of CmoCh02G010330.1 vs. ExPASy TrEMBL
Match: A0A0A0KL23 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G171720 PE=4 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 3.6e-131
Identity = 239/287 (83.28%), Postives = 262/287 (91.29%), Query Frame = 0


Query: 6   LVRNSLTLS-FFNRGLSSHAGRLEGKIALITGAASGIGKATASKFISNGAKVVLADVEEE 65
           L  NSLT S FFNR  SS   RL+GKIALITGAASGIGKATA KFISNGAKVV+AD++E+
Sbjct: 10  LFNNSLTWSFFFNRRFSSQPARLDGKIALITGAASGIGKATAEKFISNGAKVVIADIKEK 69

Query: 66  LGQATAKQLGPNATFIPCDVSNEANVSDAVDFALSLHRRLDIMYNNAGIACNTPPSILDL 125
           LGQ TAKQLGPNATFI CDV+ E+N+SDAVDF +SLH++LDIMYNNAGIACNTPPSI DL
Sbjct: 70  LGQDTAKQLGPNATFIHCDVTKESNISDAVDFTISLHKKLDIMYNNAGIACNTPPSISDL 129

Query: 126 DLAIFDKVMNINVRGVVAGIKHASRVMIPQQSGSILCTASVTGVMGGLAQHTYSVSKVAV 185
           DLA+FDKVMN+NVRGV+AG+KHASRVMIPQ+SGSILCTASVTG+MGGLAQHTYSVSKVAV
Sbjct: 130 DLAVFDKVMNVNVRGVLAGVKHASRVMIPQRSGSILCTASVTGLMGGLAQHTYSVSKVAV 189

Query: 186 IGIVKSMASELCKYGIRVNCISPFAIPTPFSINEMCRFFPGTEKEKLEKMIFDLSALEGA 245
           IGIVKS+ASELC+YGIRVNCISPF IPTPF I EM + FP  E+ KLEKMIFDLSALEG 
Sbjct: 190 IGIVKSLASELCRYGIRVNCISPFPIPTPFVIEEMVQLFPRVEEAKLEKMIFDLSALEGT 249

Query: 246 VCETNDIANAALYLASDDAKYVSGHNLVVDGSFTCFKRLNFPLPDQN 292
           VCETNDIANAAL+LASDDAKYVSGHNLVVDG+FTCFK LNFPLPDQN
Sbjct: 250 VCETNDIANAALFLASDDAKYVSGHNLVVDGAFTCFKSLNFPLPDQN 296

[Close]

BLAST of CmoCh02G010330.1 vs. NCBI nr
Match: XP_022958468.1 (secoisolariciresinol dehydrogenase [Cucurbita moschata])

HSP 1 Score: 575.9 bits (1483), Expect = 2.0e-160
Identity = 293/293 (100.00%), Postives = 293/293 (100.00%), Query Frame = 0


Query: 1   MIRSHLVRNSLTLSFFNRGLSSHAGRLEGKIALITGAASGIGKATASKFISNGAKVVLAD 60
           MIRSHLVRNSLTLSFFNRGLSSHAGRLEGKIALITGAASGIGKATASKFISNGAKVVLAD
Sbjct: 1   MIRSHLVRNSLTLSFFNRGLSSHAGRLEGKIALITGAASGIGKATASKFISNGAKVVLAD 60

Query: 61  VEEELGQATAKQLGPNATFIPCDVSNEANVSDAVDFALSLHRRLDIMYNNAGIACNTPPS 120
           VEEELGQATAKQLGPNATFIPCDVSNEANVSDAVDFALSLHRRLDIMYNNAGIACNTPPS
Sbjct: 61  VEEELGQATAKQLGPNATFIPCDVSNEANVSDAVDFALSLHRRLDIMYNNAGIACNTPPS 120

Query: 121 ILDLDLAIFDKVMNINVRGVVAGIKHASRVMIPQQSGSILCTASVTGVMGGLAQHTYSVS 180
           ILDLDLAIFDKVMNINVRGVVAGIKHASRVMIPQQSGSILCTASVTGVMGGLAQHTYSVS
Sbjct: 121 ILDLDLAIFDKVMNINVRGVVAGIKHASRVMIPQQSGSILCTASVTGVMGGLAQHTYSVS 180

Query: 181 KVAVIGIVKSMASELCKYGIRVNCISPFAIPTPFSINEMCRFFPGTEKEKLEKMIFDLSA 240
           KVAVIGIVKSMASELCKYGIRVNCISPFAIPTPFSINEMCRFFPGTEKEKLEKMIFDLSA
Sbjct: 181 KVAVIGIVKSMASELCKYGIRVNCISPFAIPTPFSINEMCRFFPGTEKEKLEKMIFDLSA 240

Query: 241 LEGAVCETNDIANAALYLASDDAKYVSGHNLVVDGSFTCFKRLNFPLPDQNPS 294
           LEGAVCETNDIANAALYLASDDAKYVSGHNLVVDGSFTCFKRLNFPLPDQNPS
Sbjct: 241 LEGAVCETNDIANAALYLASDDAKYVSGHNLVVDGSFTCFKRLNFPLPDQNPS 293

[Close]

BLAST of CmoCh02G010330.1 vs. NCBI nr
Match: KAG7035589.1 (hypothetical protein SDJN02_02386, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 566.2 bits (1458), Expect = 1.6e-157
Identity = 286/293 (97.61%), Postives = 290/293 (98.98%), Query Frame = 0


Query: 1   MIRSHLVRNSLTLSFFNRGLSSHAGRLEGKIALITGAASGIGKATASKFISNGAKVVLAD 60
           MIRSHLVRNSLTLSFFNRGLSSHAGRLEGKIALITGAASGIGKATASKFISNGAKVVLAD
Sbjct: 1   MIRSHLVRNSLTLSFFNRGLSSHAGRLEGKIALITGAASGIGKATASKFISNGAKVVLAD 60

Query: 61  VEEELGQATAKQLGPNATFIPCDVSNEANVSDAVDFALSLHRRLDIMYNNAGIACNTPPS 120
           VEEELGQATAKQLGPNATFIPCDVSNEANVSDAVDFALSLH+RLDIMYNNAGIACNTPPS
Sbjct: 61  VEEELGQATAKQLGPNATFIPCDVSNEANVSDAVDFALSLHKRLDIMYNNAGIACNTPPS 120

Query: 121 ILDLDLAIFDKVMNINVRGVVAGIKHASRVMIPQQSGSILCTASVTGVMGGLAQHTYSVS 180
           ILDLDLA+FDKVMNINVRGV+AGIKHASRVMIPQQSGSILCTASVTGVMGGLAQHTYSVS
Sbjct: 121 ILDLDLAVFDKVMNINVRGVLAGIKHASRVMIPQQSGSILCTASVTGVMGGLAQHTYSVS 180

Query: 181 KVAVIGIVKSMASELCKYGIRVNCISPFAIPTPFSINEMCRFFPGTEKEKLEKMIFDLSA 240
           KVAV GIVKSMASELCKYGIRVNCISPFAIPTPFSINEMCRFFPGTEKEKLEKMIFDLSA
Sbjct: 181 KVAVTGIVKSMASELCKYGIRVNCISPFAIPTPFSINEMCRFFPGTEKEKLEKMIFDLSA 240

Query: 241 LEGAVCETNDIANAALYLASDDAKYVSGHNLVVDGSFTCFKRLNFPLPDQNPS 294
           LEGAVCETNDIANAALYLASDDAKYVSGHNLVVDG FTCFKRLN PLPDQNP+
Sbjct: 241 LEGAVCETNDIANAALYLASDDAKYVSGHNLVVDGGFTCFKRLNLPLPDQNPT 293

[Close]

BLAST of CmoCh02G010330.1 vs. NCBI nr
Match: KAG6605680.1 (hypothetical protein SDJN03_02997, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 564.3 bits (1453), Expect = 6.1e-157
Identity = 285/293 (97.27%), Postives = 289/293 (98.63%), Query Frame = 0


Query: 1   MIRSHLVRNSLTLSFFNRGLSSHAGRLEGKIALITGAASGIGKATASKFISNGAKVVLAD 60
           MIRSHLVRNSLTLSFFNRGLSSHAGRLEGKIALITGAASGIGKATASKFISNGAKVVLAD
Sbjct: 1   MIRSHLVRNSLTLSFFNRGLSSHAGRLEGKIALITGAASGIGKATASKFISNGAKVVLAD 60

Query: 61  VEEELGQATAKQLGPNATFIPCDVSNEANVSDAVDFALSLHRRLDIMYNNAGIACNTPPS 120
           VEEELGQATAKQLGPNATFIPCDVSNEANVSDAVDFALSLH+RLDIMYNNAGIACNTPPS
Sbjct: 61  VEEELGQATAKQLGPNATFIPCDVSNEANVSDAVDFALSLHKRLDIMYNNAGIACNTPPS 120

Query: 121 ILDLDLAIFDKVMNINVRGVVAGIKHASRVMIPQQSGSILCTASVTGVMGGLAQHTYSVS 180
           ILDLDLA+FDKVMNINVRGV+AGIKHASRVMIPQQSGSILCTASVTGVMGGLAQHTY VS
Sbjct: 121 ILDLDLAVFDKVMNINVRGVLAGIKHASRVMIPQQSGSILCTASVTGVMGGLAQHTYCVS 180

Query: 181 KVAVIGIVKSMASELCKYGIRVNCISPFAIPTPFSINEMCRFFPGTEKEKLEKMIFDLSA 240
           KVAV GIVKSMASELCKYGIRVNCISPFAIPTPFSINEMCRFFPGTEKEKLEKMIFDLSA
Sbjct: 181 KVAVTGIVKSMASELCKYGIRVNCISPFAIPTPFSINEMCRFFPGTEKEKLEKMIFDLSA 240

Query: 241 LEGAVCETNDIANAALYLASDDAKYVSGHNLVVDGSFTCFKRLNFPLPDQNPS 294
           LEGAVCETNDIANAALYLASDDAKYVSGHNLVVDG FTCFKRLN PLPDQNP+
Sbjct: 241 LEGAVCETNDIANAALYLASDDAKYVSGHNLVVDGGFTCFKRLNLPLPDQNPT 293

[Close]

BLAST of CmoCh02G010330.1 vs. NCBI nr
Match: XP_023534149.1 (secoisolariciresinol dehydrogenase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 562.8 bits (1449), Expect = 1.8e-156
Identity = 284/293 (96.93%), Postives = 289/293 (98.63%), Query Frame = 0


Query: 1   MIRSHLVRNSLTLSFFNRGLSSHAGRLEGKIALITGAASGIGKATASKFISNGAKVVLAD 60
           MIRSHL+RNSLTLSFFNRGLSSHAGRLEGKIALITGAASGIGKATASKFISNGAKVVLAD
Sbjct: 1   MIRSHLLRNSLTLSFFNRGLSSHAGRLEGKIALITGAASGIGKATASKFISNGAKVVLAD 60

Query: 61  VEEELGQATAKQLGPNATFIPCDVSNEANVSDAVDFALSLHRRLDIMYNNAGIACNTPPS 120
           VEEELGQATAKQLGPNATFIPCDVSNEANVSDAVDFALSLH+RLDIMYNNAGIACNTPPS
Sbjct: 61  VEEELGQATAKQLGPNATFIPCDVSNEANVSDAVDFALSLHKRLDIMYNNAGIACNTPPS 120

Query: 121 ILDLDLAIFDKVMNINVRGVVAGIKHASRVMIPQQSGSILCTASVTGVMGGLAQHTYSVS 180
           ILDLDLA+FDKVMNINVRGV+AGIKHASRVMIPQQSGSILCTASVTGVMGGLAQHTYSVS
Sbjct: 121 ILDLDLAVFDKVMNINVRGVLAGIKHASRVMIPQQSGSILCTASVTGVMGGLAQHTYSVS 180

Query: 181 KVAVIGIVKSMASELCKYGIRVNCISPFAIPTPFSINEMCRFFPGTEKEKLEKMIFDLSA 240
           KVAVIGIVKSMASELCKYGIRVNCISPF IPTPF INEMCR FPGTEKEKLEKMIFDLSA
Sbjct: 181 KVAVIGIVKSMASELCKYGIRVNCISPFPIPTPFVINEMCRLFPGTEKEKLEKMIFDLSA 240

Query: 241 LEGAVCETNDIANAALYLASDDAKYVSGHNLVVDGSFTCFKRLNFPLPDQNPS 294
           LEGAVCETNDIANAALYLASDDAKYVSGHNLVVDG FTCFKRLNFPLPDQNP+
Sbjct: 241 LEGAVCETNDIANAALYLASDDAKYVSGHNLVVDGGFTCFKRLNFPLPDQNPT 293

[Close]

BLAST of CmoCh02G010330.1 vs. NCBI nr
Match: XP_022995920.1 (secoisolariciresinol dehydrogenase [Cucurbita maxima])

HSP 1 Score: 559.3 bits (1440), Expect = 2.0e-155
Identity = 282/293 (96.25%), Postives = 289/293 (98.63%), Query Frame = 0


Query: 1   MIRSHLVRNSLTLSFFNRGLSSHAGRLEGKIALITGAASGIGKATASKFISNGAKVVLAD 60
           MIRSHL+RNSLTLSFFNRGLSSHAGRLEGKIALITGAASGIGKATASKFISNGAKVVLAD
Sbjct: 1   MIRSHLLRNSLTLSFFNRGLSSHAGRLEGKIALITGAASGIGKATASKFISNGAKVVLAD 60

Query: 61  VEEELGQATAKQLGPNATFIPCDVSNEANVSDAVDFALSLHRRLDIMYNNAGIACNTPPS 120
           VEEELGQATAKQLGPNATFIPCDVSNE+NVSDAVDFALSLH+RLDIM NNAGIACNTPPS
Sbjct: 61  VEEELGQATAKQLGPNATFIPCDVSNESNVSDAVDFALSLHKRLDIMCNNAGIACNTPPS 120

Query: 121 ILDLDLAIFDKVMNINVRGVVAGIKHASRVMIPQQSGSILCTASVTGVMGGLAQHTYSVS 180
           ILDLDLA+FDKVMNINVRGV+AGIKHASRVMIPQQSGSILCTASVTGVMGGLAQHTYSVS
Sbjct: 121 ILDLDLAVFDKVMNINVRGVLAGIKHASRVMIPQQSGSILCTASVTGVMGGLAQHTYSVS 180

Query: 181 KVAVIGIVKSMASELCKYGIRVNCISPFAIPTPFSINEMCRFFPGTEKEKLEKMIFDLSA 240
           KVAVIGIVKSMASELCKYGIRVNCISPFAIPTPF+INEMC  FPGTEKEKLEKMIFDLSA
Sbjct: 181 KVAVIGIVKSMASELCKYGIRVNCISPFAIPTPFAINEMCHLFPGTEKEKLEKMIFDLSA 240

Query: 241 LEGAVCETNDIANAALYLASDDAKYVSGHNLVVDGSFTCFKRLNFPLPDQNPS 294
           LEGAVCETNDIANAALYLASDDAKYVSGHNLVVDG FTCFKRLNFPLPDQNP+
Sbjct: 241 LEGAVCETNDIANAALYLASDDAKYVSGHNLVVDGGFTCFKRLNFPLPDQNPT 293

[Close]

BLAST of CmoCh02G010330.1 vs. TAIR 10
Match: AT4G03140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 372.5 bits (955), Expect = 3.2e-103
Identity = 180/287 (62.72%), Postives = 233/287 (81.18%), Query Frame = 0


Query: 10  SLTLSFFNRGLSSHAG------RLEGKIALITGAASGIGKATASKFISNGAKVVLADVEE 69
           +++ +F  +G+SS +       +LEGK+ALITG ASGIGKATA KFIS+GAKV++AD++ 
Sbjct: 55  NVSKNFLIKGISSSSSSHSTSRKLEGKVALITGGASGIGKATAGKFISHGAKVIIADIQP 114

Query: 70  ELGQATAKQLGPNATFIPCDVSNEANVSDAVDFALSLHRRLDIMYNNAGIACNTPPSILD 129
           ++G+ T ++LGP+  + PCDV+ E+++++AVDFA+SLH +LDIMYNNAGI C TPPSI+D
Sbjct: 115 QIGRETEQELGPSCAYFPCDVTKESDIANAVDFAVSLHTKLDIMYNNAGIPCKTPPSIVD 174

Query: 130 LDLAIFDKVMNINVRGVVAGIKHASRVMIPQQSGSILCTASVTGVMGGLAQHTYSVSKVA 189
           LDL +FDKV+N NVRGV+AGIKHA+RVMIP+ SGSI+C  SVTG+MGGLAQHTYSVSK A
Sbjct: 175 LDLNVFDKVINTNVRGVMAGIKHAARVMIPRNSGSIICAGSVTGMMGGLAQHTYSVSKSA 234

Query: 190 VIGIVKSMASELCKYGIRVNCISPFAIPTPFSINEMCRFFPGTEKEKLEKMIFDLSALEG 249
           VIGIV+S ASELCK+ IRVNCISPFAI T F ++EM + +PG +  +L +++     L G
Sbjct: 235 VIGIVRSTASELCKHRIRVNCISPFAITTSFVMDEMRQIYPGVDDSRLIQIVQSTGVLNG 294

Query: 250 AVCETNDIANAALYLASDDAKYVSGHNLVVDGSFTCFKRLNFPLPDQ 291
            VCE  D+ANAA+YLASDD+KYV+GHNLVVDG FT  K L+FP PDQ
Sbjct: 295 EVCEPTDVANAAVYLASDDSKYVNGHNLVVDGGFTTVKTLDFPAPDQ 341

[Close]

BLAST of CmoCh02G010330.1 vs. TAIR 10
Match: AT3G26760.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 303.5 bits (776), Expect = 1.8e-82
Identity = 148/269 (55.02%), Postives = 200/269 (74.35%), Query Frame = 0


Query: 21  SSHAGRLEGKIALITGAASGIGKATASKFISNGAKVVLADVEEELGQATAKQLGPNATFI 80
           S+ + +LEGK+A+ITG ASGIGKATA +F+S GA+V++ D++EE G   A +LG  A F+
Sbjct: 30  STSSRKLEGKVAVITGGASGIGKATAEEFVSQGAQVIIVDIDEEAGHMVATELGSAAHFL 89

Query: 81  PCDVSNEANVSDAVDFALSLHRRLDIMYNNAGIACN-TPPSILDLDLAIFDKVMNINVRG 140
            CDV+ E  ++ AV+ A++ H +LD+M N+AGI+C+ +PPSI DLD+  +DKVM +NVRG
Sbjct: 90  RCDVTEEEQIAKAVETAVTRHGKLDVMLNSAGISCSISPPSIADLDMDTYDKVMRLNVRG 149

Query: 141 VVAGIKHASRVMIPQQSGSILCTASVTGVMGGLAQHTYSVSKVAVIGIVKSMASELCKYG 200
            V GIKHA+R MIP  SGSILC +S++G+MGGL  H YS+SK  + G+VK++ASELCK+G
Sbjct: 150 TVLGIKHAARAMIPAGSGSILCLSSISGLMGGLGPHAYSISKFTIPGVVKTVASELCKHG 209

Query: 201 IRVNCISPFAIPTPFSINEMCRFFPG--TEKEKLEKMIFDLSALEGAVCETNDIANAALY 260
           +R+NCISP  IPTP ++      F G    +E+L  ++     L+G  CE  D+A AALY
Sbjct: 210 LRINCISPAGIPTPLTLRMFREAFAGHSIREEQLLAIVNATGELKGEKCEEIDVAKAALY 269

Query: 261 LASDDAKYVSGHNLVVDGSFTCFKRLNFP 287
           LASDDAK+V+GHNLVVDG FTCFK LN P
Sbjct: 270 LASDDAKFVTGHNLVVDGGFTCFKSLNLP 298

[Close]

BLAST of CmoCh02G010330.1 vs. TAIR 10
Match: AT3G26770.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 300.1 bits (767), Expect = 2.0e-81
Identity = 148/274 (54.01%), Postives = 201/274 (73.36%), Query Frame = 0


Query: 19  GLSSHAGRLEGKIALITGAASGIGKATASKFISNGAKVVLADVEEELGQATAKQLGPNAT 78
           G +  + +LEGK+ALITG ASG+GKATAS+F+ +GA+VV+AD++ E G  TAK+LG  A 
Sbjct: 33  GCTCTSKKLEGKVALITGGASGLGKATASEFLRHGARVVIADLDAETGTKTAKELGSEAE 92

Query: 79  FIPCDVSNEANVSDAVDFALSLHRRLDIMYNNAGI-ACNTPPSILDLDLAIFDKVMNINV 138
           F+ CDV+ EA+++ AV+  +  + +LD+MYNNAGI    TP SI  LD+  F++VM INV
Sbjct: 93  FVRCDVTVEADIAGAVEMTVERYGKLDVMYNNAGIVGPMTPASISQLDMTEFERVMRINV 152

Query: 139 RGVVAGIKHASRVMIPQQSGSILCTASVTGVMGGLAQHTYSVSKVAVIGIVKSMASELCK 198
            GVV+GIKHA++ MIP +SG ILCT+SV GV GGLA H+Y++SK    GIVKS ASELC+
Sbjct: 153 FGVVSGIKHAAKFMIPARSGCILCTSSVAGVTGGLAPHSYTISKFTTPGIVKSAASELCE 212

Query: 199 YGIRVNCISPFAIPTPFSINEMCRFFPGTEKEKLEKMIFDLSALEGAVCETNDIANAALY 258
           +G+R+NCISP  + TP +++ + + FP   +EKL + +  +  L+GA CE  D+A AALY
Sbjct: 213 HGVRINCISPGTVATPLTLSYLQKVFPKVSEEKLRETVKGMGELKGAECEEADVAKAALY 272

Query: 259 LASDDAKYVSGHNLVVDGSFTCFKRLNFPLPDQN 292
           LAS+D KYV+GHNLVVDG  T FK   FP P  +
Sbjct: 273 LASNDGKYVTGHNLVVDGGMTAFKIAGFPFPSDS 306

[Close]

BLAST of CmoCh02G010330.1 vs. TAIR 10
Match: AT3G51680.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 235.7 bits (600), Expect = 4.6e-62
Identity = 132/265 (49.81%), Postives = 172/265 (64.91%), Query Frame = 0


Query: 26  RLEGKIALITGAASGIGKATASKFISNGAKVVLADVEEELGQATAKQL-----GPNATFI 85
           RLEGK+A+ITG A GIGKAT   F  +GA VV+ADV+   G + AK L      P   FI
Sbjct: 31  RLEGKVAIITGGAHGIGKATVMLFARHGATVVIADVDNVAGSSLAKSLSSHKTSPMVAFI 90

Query: 86  PCDVSNEANVSDAVDFALSLHRRLDIMYNNAGIACNTP--PSILDLDLAIFDKVMNINVR 145
            CDVS EA+V + V+  ++ + RLDI++NNAG+  +     SILD D   FD VM +NVR
Sbjct: 91  SCDVSVEADVENLVNVTVARYGRLDILFNNAGVLGDQKKHKSILDFDADEFDHVMRVNVR 150

Query: 146 GVVAGIKHASRVMIPQQ-SGSILCTASVTGVMGGLAQHTYSVSKVAVIGIVKSMASELCK 205
           GV  G+KH +R MI +   G I+ TASV GVMGG+  H Y+ SK A++G+ K+ A EL K
Sbjct: 151 GVGLGMKHGARAMIKRGFKGCIISTASVAGVMGGMGPHAYTASKHAIVGLTKNAACELGK 210

Query: 206 YGIRVNCISPFAIPTPFSINEMCRFFPG----TEKEKLEKMIFDLSALEGAVCETNDIAN 265
           YGIRVNCISPF + T   +N   +   G     + E++E+ +  L+ L+G     NDIA 
Sbjct: 211 YGIRVNCISPFGVATSMLVNAWRKTSGGDVEDDDVEEMEEFVRSLANLKGETLRANDIAE 270

Query: 266 AALYLASDDAKYVSGHNLVVDGSFT 279
           AALYLASD++KYV+GHNLVVDG  T
Sbjct: 271 AALYLASDESKYVNGHNLVVDGGVT 295

[Close]

BLAST of CmoCh02G010330.1 vs. TAIR 10
Match: AT2G47120.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 213.4 bits (542), Expect = 2.5e-55
Identity = 117/258 (45.35%), Postives = 166/258 (64.34%), Query Frame = 0


Query: 26  RLEGKIALITGAASGIGKATASKFISNGAKVVLADVEEELGQATAKQLGPN-ATFIPCDV 85
           RLEGKI +ITG ASGIG   A  F  +GAKVV+ DV+EELGQ  A  +G + A+F  CDV
Sbjct: 5   RLEGKIVIITGGASGIGADAARLFTDHGAKVVIVDVQEELGQNVAVLIGKDKASFYRCDV 64

Query: 86  SNEANVSDAVDFALSLHRRLDIMYNNAGIACNTPPSILDLDLAIFDKVMNINVRGVVAGI 145
           +NE  V DAV F +  H +LD++++NAG+      S LD DL  FD++M +NVRG  A I
Sbjct: 65  TNETEVEDAVKFTVEKHGKLDVLFSNAGV-LEPLESFLDFDLERFDRIMAVNVRGAAAFI 124

Query: 146 KHASRVMIPQQS-GSILCTASVTGVMGGLAQHTYSVSKVAVIGIVKSMASELCKYGIRVN 205
           KHA+R M+ + + GSI+CT SV+  +GG   H Y+ SK  ++G+++S   +L KYGIRVN
Sbjct: 125 KHAARAMVEKGTRGSIVCTTSVSAEIGG-GHHGYTASKHGLVGLIRSACGDLGKYGIRVN 184

Query: 206 CISPFAIPTPFSINEMCRFFPGTEKEKLEKMIFDLSALEGAVCETNDIANAALYLASDDA 265
            ++P+A+ TP + ++          ++LE        L+G V + + +A  AL+LASDD+
Sbjct: 185 GVAPYAVATPMTSHD------EVTGKQLEDYFDAKGILKGMVLKASHVAQVALFLASDDS 244

Query: 266 KYVSGHNLVVDGSFTCFK 282
            Y+SG NL VDG +T  K
Sbjct: 245 AYISGQNLAVDGGYTVVK 254

The following BLAST results are available for this feature:

BLAST of CmoCh02G010330.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94KL7	4.1e-63	49.81	Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 P...	[more]
Q9SCU0	6.5e-61	49.81	Short-chain dehydrogenase reductase 2a OS=Arabidopsis thaliana OX=3702 GN=SDR2a ...	[more]
P50160	1.2e-59	44.33	Sex determination protein tasselseed-2 OS=Zea mays OX=4577 GN=TS2 PE=2 SV=1	[more]
H9BFQ0	4.4e-57	47.27	Tropinone reductase-like 1 OS=Erythroxylum coca OX=289672 PE=2 SV=1	[more]
F1SWA0	7.5e-57	46.15	Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1	[more]

BLAST of CmoCh02G010330.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H354	9.8e-161	100.00	secoisolariciresinol dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111459685...	[more]
A0A6J1K592	9.5e-156	96.25	secoisolariciresinol dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111491301 P...	[more]
A0A6J1DLW0	1.5e-132	83.99	secoisolariciresinol dehydrogenase OS=Momordica charantia OX=3673 GN=LOC11102177...	[more]
A0A1S3CT49	2.5e-132	84.32	secoisolariciresinol dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103504658 PE=4 ...	[more]
A0A0A0KL23	3.6e-131	83.28	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G171720 PE=4 SV=1	[more]

BLAST of CmoCh02G010330.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022958468.1	2.0e-160	100.00	secoisolariciresinol dehydrogenase [Cucurbita moschata]	[more]
KAG7035589.1	1.6e-157	97.61	hypothetical protein SDJN02_02386, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6605680.1	6.1e-157	97.27	hypothetical protein SDJN03_02997, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023534149.1	1.8e-156	96.93	secoisolariciresinol dehydrogenase [Cucurbita pepo subsp. pepo]	[more]
XP_022995920.1	2.0e-155	96.25	secoisolariciresinol dehydrogenase [Cucurbita maxima]	[more]

BLAST of CmoCh02G010330.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G03140.1	3.2e-103	62.72	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G26760.1	1.8e-82	55.02	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G26770.1	2.0e-81	54.01	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G51680.1	4.6e-62	49.81	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G47120.1	2.5e-55	45.35	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 31..48 score: 49.49 coord: 177..196 score: 37.18 coord: 241..261 score: 29.9 coord: 102..113 score: 49.7 coord: 198..215 score: 39.71 coord: 151..167 score: 31.23
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00080	SDRFAMILY	coord: 157..165 score: 41.95 coord: 177..196 score: 35.51 coord: 102..113 score: 47.24
None	No IPR available	GENE3D	3.40.50.720		coord: 18..283 e-value: 4.6E-76 score: 257.4
None	No IPR available	PFAM	PF13561	adh_short_C2	coord: 36..277 e-value: 1.7E-61 score: 207.8
None	No IPR available	PIRSR	PIRSR000094-3	PIRSR000094-3	coord: 25..278 e-value: 1.2E-16 score: 58.5
None	No IPR available	PIRSR	PIRSR000094-2	PIRSR000094-2	coord: 25..278 e-value: 1.2E-16 score: 58.5
None	No IPR available	PIRSR	PIRSR000095-1	PIRSR000095-1	coord: 33..218 e-value: 5.8E-24 score: 82.6
None	No IPR available	PANTHER	PTHR43180:SF55	ALCOHOL DEHYDROGENASE-LIKE PROTEIN	coord: 16..289
None	No IPR available	PANTHER	PTHR43180	3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)	coord: 16..289
IPR020904	Short-chain dehydrogenase/reductase, conserved site	PROSITE	PS00061	ADH_SHORT	coord: 164..192
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 28..278

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmoCh02G010330	CmoCh02G010330	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh02G010330.1:exon:4847	CmoCh02G010330.1:exon:4847	exon
CmoCh02G010330.1:exon:4848	CmoCh02G010330.1:exon:4848	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh02G010330.1:five_prime_utr	CmoCh02G010330.1:five_prime_utr	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh02G010330.1:cds	CmoCh02G010330.1:cds	CDS
CmoCh02G010330.1:cds	CmoCh02G010330.1:cds_2	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh02G010330.1:three_prime_utr	CmoCh02G010330.1:three_prime_utr	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmoCh02G010330.1	CmoCh02G010330.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity