CmoCh02G008600.1 (mRNA) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh02G008600.1
Type	mRNA
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	DNA repair protein RAD51-like protein 2
Location	Cmo_Chr02: 5239238 .. 5239879 (-)
Sequence length	357
RNA-Seq Expression	CmoCh02G008600.1
Synteny	CmoCh02G008600.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCGACTTTTTCTGGTTGTTGAATTTCAGTTTTGCTTGAAACTCTCTTTATTGGCTGCTCTGCCACCGAGCTATGGAAGTTTAGATGGCAGAGTAATATATATTGATGTAGAATCTAAGTTTAGTTCCAGAAGGTCTTTTAATTATACCATGCTACTGGTTGTTTACTCATTATATTTGTGTAATGGGCGCATGACTTTTTTGGTTTTTCAACTCCGTCTCTCTCGGCAGGATGATTGAAATTGACATGAGAAGCTTTCCTGATGTATTGGAAATGAAGGACATAGCACAAGAGGTATCAAACTGATATATATTCTCTTCCTTTCAAATTTTAATTTAAAATGCATAAGATGGATCATTCTCAATTTTCATTCCAGATGGCTGGTTTGTCTTGCCACCTGCTTCACTTTCTGAATTCAATGAGAGGTTTGGTTCAAAGTCAAATAAATGTCATTTGGCCTAGACATGTCAATGATTTGAGAAAATGAAATGCTAAGGATAACACATTTAATCTACAAAATTTAGCAGAATATTTAGACGCATGTAATGCAGTTCGAGGTATGTACATATTAGCTCAATTGCAATGATTTACCTGCAATGGCATGGAATATTCAAATAGAACACAATTCAGGTGCTTGTTAG

mRNA sequence

ATGCGACTTTTTCTGGTTGTTGAATTTCAGTTTTGCTTGAAACTCTCTTTATTGGCTGCTCTGCCACCGAGCTATGGAAGTTTAGATGGCAGAGTAATATATATTGATGTAGAATCTAAGTTTAGTTCCAGAAGGATGATTGAAATTGACATGAGAAGCTTTCCTGATGTATTGGAAATGAAGGACATAGCACAAGAGATGGCTGGTTTGTCTTGCCACCTGCTTCACTTTCTGAATTCAATGAGAGGTTTGGTTCAAAGTCAAATAAATGTCATTTGGCCTAGACATCTCAATTGCAATGATTTACCTGCAATGGCATGGAATATTCAAATAGAACACAATTCAGGTGCTTGTTAG

Coding sequence (CDS)

ATGCGACTTTTTCTGGTTGTTGAATTTCAGTTTTGCTTGAAACTCTCTTTATTGGCTGCTCTGCCACCGAGCTATGGAAGTTTAGATGGCAGAGTAATATATATTGATGTAGAATCTAAGTTTAGTTCCAGAAGGATGATTGAAATTGACATGAGAAGCTTTCCTGATGTATTGGAAATGAAGGACATAGCACAAGAGATGGCTGGTTTGTCTTGCCACCTGCTTCACTTTCTGAATTCAATGAGAGGTTTGGTTCAAAGTCAAATAAATGTCATTTGGCCTAGACATCTCAATTGCAATGATTTACCTGCAATGGCATGGAATATTCAAATAGAACACAATTCAGGTGCTTGTTAG

Protein sequence

MRLFLVVEFQFCLKLSLLAALPPSYGSLDGRVIYIDVESKFSSRRMIEIDMRSFPDVLEMKDIAQEMAGLSCHLLHFLNSMRGLVQSQINVIWPRHLNCNDLPAMAWNIQIEHNSGAC

Homology

[Close]

BLAST of CmoCh02G008600.1 vs. ExPASy Swiss-Prot
Match: Q9SK02 (DNA repair protein RAD51 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=RAD51B PE=2 SV=2)

HSP 1 Score: 88.6 bits (218), Expect = 5.2e-17
Identity = 41/60 (68.33%), Postives = 51/60 (85.00%), Query Frame = 0


Query: 10  QFCLKLSLLAALPPSYGSLDGRVIYIDVESKFSSRRMIEIDMRSFPDVLEMKDIAQEMAG 69
           QFC+KL+L A+ P +YG LDGRVIYIDVESKFSSRR+IE+ + SFP+V  +K +AQEMAG
Sbjct: 117 QFCMKLALSASFPVAYGGLDGRVIYIDVESKFSSRRVIEMGLESFPEVFHLKGMAQEMAG 176

[Close]

BLAST of CmoCh02G008600.1 vs. ExPASy Swiss-Prot
Match: O15315 (DNA repair protein RAD51 homolog 2 OS=Homo sapiens OX=9606 GN=RAD51B PE=1 SV=2)

HSP 1 Score: 55.8 bits (133), Expect = 3.7e-07
Identity = 24/46 (52.17%), Postives = 33/46 (71.74%), Query Frame = 0


Query: 10  QFCLKLSLLAALPPSYGSLDGRVIYIDVESKFSSRRMIEIDMRSFP 56
           QFC+ +S+LA LP + G L+G V+YID ES FS+ R++EI    FP
Sbjct: 116 QFCIMMSILATLPTNMGGLEGAVVYIDTESAFSAERLVEIAESRFP 161

[Close]

BLAST of CmoCh02G008600.1 vs. ExPASy Swiss-Prot
Match: O35719 (DNA repair protein RAD51 homolog 2 OS=Mus musculus OX=10090 GN=Rad51b PE=2 SV=2)

HSP 1 Score: 55.8 bits (133), Expect = 3.7e-07
Identity = 24/53 (45.28%), Postives = 35/53 (66.04%), Query Frame = 0


Query: 10  QFCLKLSLLAALPPSYGSLDGRVIYIDVESKFSSRRMIEIDMRSFPDVLEMKD 63
           QFC+ +S+LA LP S G L+G V+YID ES F++ R++EI    FP     ++
Sbjct: 116 QFCIMMSVLATLPTSLGGLEGAVVYIDTESAFTAERLVEIAESRFPQYFNTEE 168

[Close]

BLAST of CmoCh02G008600.1 vs. ExPASy TrEMBL
Match: A0A6J1CY45 (DNA repair protein RAD51 homolog 2 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111015517 PE=4 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 8.9e-20
Identity = 53/60 (88.33%), Postives = 54/60 (90.00%), Query Frame = 0


Query: 10  QFCLKLSLLAALPPSYGSLDGRVIYIDVESKFSSRRMIEIDMRSFPDVLEMKDIAQEMAG 69
           QFCLKLSLLAALPPSYG L GRVIYIDVESKFSSRRMIEI MRSFPDV   KD+AQEMAG
Sbjct: 117 QFCLKLSLLAALPPSYGGLGGRVIYIDVESKFSSRRMIEIGMRSFPDVFHKKDMAQEMAG 176

[Close]

BLAST of CmoCh02G008600.1 vs. ExPASy TrEMBL
Match: A0A0A0KFZ1 (RECA_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G296460 PE=4 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 8.9e-20
Identity = 52/60 (86.67%), Postives = 54/60 (90.00%), Query Frame = 0


Query: 10  QFCLKLSLLAALPPSYGSLDGRVIYIDVESKFSSRRMIEIDMRSFPDVLEMKDIAQEMAG 69
           QFCLKLS LAALPPSYG LDGRVIYIDVESKFSS+RMIEI MRSFPDV   KD+AQEMAG
Sbjct: 117 QFCLKLSFLAALPPSYGGLDGRVIYIDVESKFSSKRMIEIGMRSFPDVFNKKDMAQEMAG 176

[Close]

BLAST of CmoCh02G008600.1 vs. ExPASy TrEMBL
Match: A0A6J1CY51 (DNA repair protein RAD51 homolog 2 isoform X4 OS=Momordica charantia OX=3673 GN=LOC111015517 PE=4 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 8.9e-20
Identity = 53/60 (88.33%), Postives = 54/60 (90.00%), Query Frame = 0


Query: 10  QFCLKLSLLAALPPSYGSLDGRVIYIDVESKFSSRRMIEIDMRSFPDVLEMKDIAQEMAG 69
           QFCLKLSLLAALPPSYG L GRVIYIDVESKFSSRRMIEI MRSFPDV   KD+AQEMAG
Sbjct: 117 QFCLKLSLLAALPPSYGGLGGRVIYIDVESKFSSRRMIEIGMRSFPDVFHKKDMAQEMAG 176

[Close]

BLAST of CmoCh02G008600.1 vs. ExPASy TrEMBL
Match: A0A6J1CWT5 (DNA repair protein RAD51 homolog 2 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111015517 PE=4 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 8.9e-20
Identity = 53/60 (88.33%), Postives = 54/60 (90.00%), Query Frame = 0


Query: 10  QFCLKLSLLAALPPSYGSLDGRVIYIDVESKFSSRRMIEIDMRSFPDVLEMKDIAQEMAG 69
           QFCLKLSLLAALPPSYG L GRVIYIDVESKFSSRRMIEI MRSFPDV   KD+AQEMAG
Sbjct: 79  QFCLKLSLLAALPPSYGGLGGRVIYIDVESKFSSRRMIEIGMRSFPDVFHKKDMAQEMAG 138

[Close]

BLAST of CmoCh02G008600.1 vs. ExPASy TrEMBL
Match: A0A6J1CYS7 (DNA repair protein RAD51 homolog 2 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111015517 PE=4 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 8.9e-20
Identity = 53/60 (88.33%), Postives = 54/60 (90.00%), Query Frame = 0


Query: 10  QFCLKLSLLAALPPSYGSLDGRVIYIDVESKFSSRRMIEIDMRSFPDVLEMKDIAQEMAG 69
           QFCLKLSLLAALPPSYG L GRVIYIDVESKFSSRRMIEI MRSFPDV   KD+AQEMAG
Sbjct: 117 QFCLKLSLLAALPPSYGGLGGRVIYIDVESKFSSRRMIEIGMRSFPDVFHKKDMAQEMAG 176

[Close]

BLAST of CmoCh02G008600.1 vs. NCBI nr
Match: KAG6605511.1 (DNA repair protein RAD51-like 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 116.7 bits (291), Expect = 1.4e-22
Identity = 59/70 (84.29%), Postives = 63/70 (90.00%), Query Frame = 0


Query: 4   FLVVEFQFCLKLSLLAALPPSYGSLDGRVIYIDVESKFSSRRMIEIDMRSFPDVLEMKDI 63
           ++   F+FCLKLSLLAALPPSYGSLDGRVIYIDVESKFSSRRMIEIDMRSFPDVLEMKDI
Sbjct: 122 YMFKSFRFCLKLSLLAALPPSYGSLDGRVIYIDVESKFSSRRMIEIDMRSFPDVLEMKDI 181

Query: 64  AQEMAGLSCH 74
           AQE    +CH
Sbjct: 182 AQE--DTACH 189

[Close]

BLAST of CmoCh02G008600.1 vs. NCBI nr
Match: XP_038901711.1 (DNA repair protein RAD51 homolog 2 isoform X3 [Benincasa hispida])

HSP 1 Score: 107.5 bits (267), Expect = 8.2e-20
Identity = 53/60 (88.33%), Postives = 54/60 (90.00%), Query Frame = 0


Query: 10  QFCLKLSLLAALPPSYGSLDGRVIYIDVESKFSSRRMIEIDMRSFPDVLEMKDIAQEMAG 69
           QFCLKLS LAALPPSYG LDGRVIYIDVESKFSSRRMIEI MRSFPDV   KD+AQEMAG
Sbjct: 117 QFCLKLSFLAALPPSYGGLDGRVIYIDVESKFSSRRMIEIGMRSFPDVFHKKDMAQEMAG 176

[Close]

BLAST of CmoCh02G008600.1 vs. NCBI nr
Match: XP_038901712.1 (DNA repair protein RAD51 homolog 2 isoform X4 [Benincasa hispida] >XP_038901713.1 DNA repair protein RAD51 homolog 2 isoform X4 [Benincasa hispida])

HSP 1 Score: 107.5 bits (267), Expect = 8.2e-20
Identity = 53/60 (88.33%), Postives = 54/60 (90.00%), Query Frame = 0


Query: 10  QFCLKLSLLAALPPSYGSLDGRVIYIDVESKFSSRRMIEIDMRSFPDVLEMKDIAQEMAG 69
           QFCLKLS LAALPPSYG LDGRVIYIDVESKFSSRRMIEI MRSFPDV   KD+AQEMAG
Sbjct: 117 QFCLKLSFLAALPPSYGGLDGRVIYIDVESKFSSRRMIEIGMRSFPDVFHKKDMAQEMAG 176

[Close]

BLAST of CmoCh02G008600.1 vs. NCBI nr
Match: XP_038901709.1 (DNA repair protein RAD51 homolog 2 isoform X2 [Benincasa hispida] >XP_038901710.1 DNA repair protein RAD51 homolog 2 isoform X2 [Benincasa hispida])

HSP 1 Score: 107.5 bits (267), Expect = 8.2e-20
Identity = 53/60 (88.33%), Postives = 54/60 (90.00%), Query Frame = 0


Query: 10  QFCLKLSLLAALPPSYGSLDGRVIYIDVESKFSSRRMIEIDMRSFPDVLEMKDIAQEMAG 69
           QFCLKLS LAALPPSYG LDGRVIYIDVESKFSSRRMIEI MRSFPDV   KD+AQEMAG
Sbjct: 117 QFCLKLSFLAALPPSYGGLDGRVIYIDVESKFSSRRMIEIGMRSFPDVFHKKDMAQEMAG 176

[Close]

BLAST of CmoCh02G008600.1 vs. NCBI nr
Match: XP_038901703.1 (DNA repair protein RAD51 homolog 2 isoform X1 [Benincasa hispida] >XP_038901704.1 DNA repair protein RAD51 homolog 2 isoform X1 [Benincasa hispida] >XP_038901705.1 DNA repair protein RAD51 homolog 2 isoform X1 [Benincasa hispida] >XP_038901707.1 DNA repair protein RAD51 homolog 2 isoform X1 [Benincasa hispida] >XP_038901708.1 DNA repair protein RAD51 homolog 2 isoform X1 [Benincasa hispida])

HSP 1 Score: 107.5 bits (267), Expect = 8.2e-20
Identity = 53/60 (88.33%), Postives = 54/60 (90.00%), Query Frame = 0


Query: 10  QFCLKLSLLAALPPSYGSLDGRVIYIDVESKFSSRRMIEIDMRSFPDVLEMKDIAQEMAG 69
           QFCLKLS LAALPPSYG LDGRVIYIDVESKFSSRRMIEI MRSFPDV   KD+AQEMAG
Sbjct: 117 QFCLKLSFLAALPPSYGGLDGRVIYIDVESKFSSRRMIEIGMRSFPDVFHKKDMAQEMAG 176

[Close]

BLAST of CmoCh02G008600.1 vs. TAIR 10
Match: AT2G28560.1 (DNA repair (Rad51) family protein )

HSP 1 Score: 88.6 bits (218), Expect = 3.7e-18
Identity = 41/60 (68.33%), Postives = 51/60 (85.00%), Query Frame = 0


Query: 10  QFCLKLSLLAALPPSYGSLDGRVIYIDVESKFSSRRMIEIDMRSFPDVLEMKDIAQEMAG 69
           QFC+KL+L A+ P +YG LDGRVIYIDVESKFSSRR+IE+ + SFP+V  +K +AQEMAG
Sbjct: 117 QFCMKLALSASFPVAYGGLDGRVIYIDVESKFSSRRVIEMGLESFPEVFHLKGMAQEMAG 176

[Close]

BLAST of CmoCh02G008600.1 vs. TAIR 10
Match: AT2G28560.2 (DNA repair (Rad51) family protein )

HSP 1 Score: 88.6 bits (218), Expect = 3.7e-18
Identity = 41/60 (68.33%), Postives = 51/60 (85.00%), Query Frame = 0


Query: 10  QFCLKLSLLAALPPSYGSLDGRVIYIDVESKFSSRRMIEIDMRSFPDVLEMKDIAQEMAG 69
           QFC+KL+L A+ P +YG LDGRVIYIDVESKFSSRR+IE+ + SFP+V  +K +AQEMAG
Sbjct: 117 QFCMKLALSASFPVAYGGLDGRVIYIDVESKFSSRRVIEMGLESFPEVFHLKGMAQEMAG 176

[Close]

BLAST of CmoCh02G008600.1 vs. TAIR 10
Match: AT2G28560.3 (DNA repair (Rad51) family protein )

HSP 1 Score: 88.6 bits (218), Expect = 3.7e-18
Identity = 41/60 (68.33%), Postives = 51/60 (85.00%), Query Frame = 0


Query: 10  QFCLKLSLLAALPPSYGSLDGRVIYIDVESKFSSRRMIEIDMRSFPDVLEMKDIAQEMAG 69
           QFC+KL+L A+ P +YG LDGRVIYIDVESKFSSRR+IE+ + SFP+V  +K +AQEMAG
Sbjct: 85  QFCMKLALSASFPVAYGGLDGRVIYIDVESKFSSRRVIEMGLESFPEVFHLKGMAQEMAG 144

[Close]

BLAST of CmoCh02G008600.1 vs. TAIR 10
Match: AT2G28560.4 (DNA repair (Rad51) family protein )

HSP 1 Score: 88.6 bits (218), Expect = 3.7e-18
Identity = 41/60 (68.33%), Postives = 51/60 (85.00%), Query Frame = 0


Query: 10  QFCLKLSLLAALPPSYGSLDGRVIYIDVESKFSSRRMIEIDMRSFPDVLEMKDIAQEMAG 69
           QFC+KL+L A+ P +YG LDGRVIYIDVESKFSSRR+IE+ + SFP+V  +K +AQEMAG
Sbjct: 85  QFCMKLALSASFPVAYGGLDGRVIYIDVESKFSSRRVIEMGLESFPEVFHLKGMAQEMAG 144

The following BLAST results are available for this feature:

BLAST of CmoCh02G008600.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SK02	5.2e-17	68.33	DNA repair protein RAD51 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=RAD51B PE=...	[more]
O15315	3.7e-07	52.17	DNA repair protein RAD51 homolog 2 OS=Homo sapiens OX=9606 GN=RAD51B PE=1 SV=2	[more]
O35719	3.7e-07	45.28	DNA repair protein RAD51 homolog 2 OS=Mus musculus OX=10090 GN=Rad51b PE=2 SV=2	[more]

BLAST of CmoCh02G008600.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CY45	8.9e-20	88.33	DNA repair protein RAD51 homolog 2 isoform X1 OS=Momordica charantia OX=3673 GN=...	[more]
A0A0A0KFZ1	8.9e-20	86.67	RECA_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G296460 PE=4...	[more]
A0A6J1CY51	8.9e-20	88.33	DNA repair protein RAD51 homolog 2 isoform X4 OS=Momordica charantia OX=3673 GN=...	[more]
A0A6J1CWT5	8.9e-20	88.33	DNA repair protein RAD51 homolog 2 isoform X2 OS=Momordica charantia OX=3673 GN=...	[more]
A0A6J1CYS7	8.9e-20	88.33	DNA repair protein RAD51 homolog 2 isoform X3 OS=Momordica charantia OX=3673 GN=...	[more]

BLAST of CmoCh02G008600.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6605511.1	1.4e-22	84.29	DNA repair protein RAD51-like 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_038901711.1	8.2e-20	88.33	DNA repair protein RAD51 homolog 2 isoform X3 [Benincasa hispida]	[more]
XP_038901712.1	8.2e-20	88.33	DNA repair protein RAD51 homolog 2 isoform X4 [Benincasa hispida] >XP_038901713....	[more]
XP_038901709.1	8.2e-20	88.33	DNA repair protein RAD51 homolog 2 isoform X2 [Benincasa hispida] >XP_038901710....	[more]
XP_038901703.1	8.2e-20	88.33	DNA repair protein RAD51 homolog 2 isoform X1 [Benincasa hispida] >XP_038901704....	[more]

BLAST of CmoCh02G008600.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G28560.1	3.7e-18	68.33	DNA repair (Rad51) family protein	[more]
AT2G28560.2	3.7e-18	68.33	DNA repair (Rad51) family protein	[more]
AT2G28560.3	3.7e-18	68.33	DNA repair (Rad51) family protein	[more]
AT2G28560.4	3.7e-18	68.33	DNA repair (Rad51) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013632	DNA recombination and repair protein Rad51-like, C-terminal	PFAM	PF08423	Rad51	coord: 10..65 e-value: 7.8E-7 score: 28.6
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 9..98 e-value: 7.4E-8 score: 34.3
IPR030548	DNA repair protein RAD51 homologue 2	PANTHER	PTHR46456	DNA REPAIR PROTEIN RAD51 HOMOLOG 2	coord: 10..69

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmoCh02G008600	CmoCh02G008600	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh02G008600.1:exon:53	CmoCh02G008600.1:exon:53	exon
CmoCh02G008600.1:exon:52	CmoCh02G008600.1:exon:52	exon
CmoCh02G008600.1:exon:51	CmoCh02G008600.1:exon:51	exon
CmoCh02G008600.1:exon:50	CmoCh02G008600.1:exon:50	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh02G008600.1:cds	CmoCh02G008600.1:cds_4	CDS
CmoCh02G008600.1:cds	CmoCh02G008600.1:cds_3	CDS
CmoCh02G008600.1:cds	CmoCh02G008600.1:cds_2	CDS
CmoCh02G008600.1:cds	CmoCh02G008600.1:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmoCh02G008600.1	CmoCh02G008600.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006281	DNA repair
biological_process	GO:0000724	double-strand break repair via homologous recombination
cellular_component	GO:0033063	Rad51B-Rad51C-Rad51D-XRCC2 complex
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0008094	ATP-dependent activity, acting on DNA