CmoCh01G007320.1 (mRNA) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh01G007320.1
TypemRNA
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionLaccase
LocationCmo_Chr01: 3780434 .. 3783309 (-)
Sequence length1772
RNA-Seq ExpressionCmoCh01G007320.1
SyntenyCmoCh01G007320.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAACATCTGTTAAAGACAAAATCTTTGTCTTCTTGCTTCTTGTTAGCCATTTTGGTCTTGATTCCTTTCGCCATTGCAGAAACTCACTACCATGAATTCATCGTAAGCTTCCAAAATCTCCATTTTCGCTAAAAAATTTCACAATTCCTCTGTTTTTCTGACTCTGTTTCGCTGCAAAATGCAGGTTCAACGCAAACCAGTGAAGAGGCTGTGCAAAGTTCATAACATTATAACAGTGAACGGACAGTTCCCTGGGCCAATTTTAGAAGTAAGAAACGGCGATTCTTTGGTCATCAAAGTGGTTAACGCTGTCCGCTACAATGTCTCTCTTCATTGGTAATTCTTTACGCAAAACCCATCTCTGTTTTTTACTGACAGAGACGTGTAATGAACTGGTTGTGGGTTTGATATGATTAGGCATGGGATCAAGCAGCTGAGGAACCCATGGGCGGATGGGCCGGAATTTATAACGCAATGCTCTATAAAGCCAGGGGGTAGCTATACTTACAGGTTCACTATTGAAGATCAAGAGGGTACTCTGTGGTGGCATGCGCATAGCCGATGGCTTCGAGCTACTGTTTATGGCGCGCTCATAATTTATCCGAAGATGGGTTCTTATTATCCGTTCTTAATGCCTAAAAGAGAAGCTTCTGTTCTTCTTGGTAATTGTTGTTCGATGTTGTTATGGGTTGGATTAATGCTGAGATTTTGACTGTTTGATGGGTGTGAAAAACAGGGGAGTGGTTTGATAGAGATCCGATGAGTGTACTGCGGCAAGCGCTGTTGAGTGGAGCGGCTCCTAATGTGTCTGATGCTTACACCATCAACGGGCAGCCCGGCGATTTCTATAGATGCTCGAAGAAAGGTCGGTGAGAGTTTAACGCTCTGAATTTTACGAAAATATGTTTCATGATGAGAACACTATCTGCTAGCGGTGAGCTTGGACTGTTACAAATGGTATCAGAGCCAAACACTGGGCGATTTCCCAGTGAAGGAGGGTGGACACGAGGCAGTGTGTTACCCACATCGATTGGAGAGCCTCAAAGGGAGTGGACACGAGGTAGTGCCAGTGAGGACACTGGACCTTGAAAGGGAGTGAATTTGGGATCTCTCATCTATTGATATTGGGGATCCCTCATCTATTGATGTAACGGCCCAGATCCATCGCTAGCAAATATTGTTCTCTTTGGACTTTCCCTTTCGGGCTTCCCCTCAAGACTTTAAACGCGTCTGCAAAGGGAACGTTTGCACACCCTTATAAAGGGTGTTTTGTTCTCCTCCCCAACCAATGTGGGACATCACAATCCACCCCCCTTCAGGGCAAAGCGTCCTCGCTAACACTCTTTCCTTTCTCCAATTGATGTGGGATCCCTACCAAATCCACCCTTTTGAGGCCCAGCGTCCTTACTGGCACACCATCTCGTGTCTTCCCCTTTGGGGAACAGCCTCCTTGCTAACAAATAGTCCGGTGTCTGGCTCTGATACCATTTGTAATGGCCCAGATTCACACCTAGCCGATATTGTCAAAGCTTTCCCTCTCAGGCTTCTCCGTCAAAACTTTAAAATGCATCTATTAGGGGAAGATTTCCACACCTTAATATTGTCAAAGCTTTCCCTTCCCGACTTCTTTTTTATTTTTATTTCAACTCTTAAAAAGTCAACCGAGCTAGATGGTGAGATTATATTGACCACTTAAAAGGTCAACTAAGCTGAAATTAACTTGATTTTGCAGAGACGGTGAGATTCGGAGTGAATGCCGGCGAGACAATCCTTCTCAGAATCGTAAACTCCGGACTGAATCAAGAACTCTTCTTCTCCGTCGCGAACCACCCATTGACAGTGGTCGCCGTCGACGCATCTTACACCAAACCATTCAAAACAAATGTCATCATGATCGGACCCGGCCAAACCACCGACGTCCTCCTCACAGCCAACCAACCTCCAGCCCATTACTACATGGCCGCCACCGCTTACAACACCGCCATGAACGCCTCCTTCGACAACACCACCACCACTGCCATTCTCCAATACAACAACAATTACCAACAACAACCAAAACCCATCCTTGCCCAGCTCCCCTTTTTCAACGACACACCCACCTCCACCCGCTTCACCGCCCAACTCCGCAGCTTAAACAGAGCAAACGTCCCAACCCAAATCGACGAATCCTTACTCTTCACCGTCGGATTAGGCCTCATCAATTGCACCAACCCCAACAGCCCCCGCTGCCAAGGCCCCAACGGCACACGCTTCGCCGCCAGCATCAACAACAACTCCTTCGTTTTCCCCAAATCCAATTCCATTATGCAGGCCTTTTACCAAGGGATTCCCGGCGTTTTCACCACCGATTTCCCCCCTGTTCCGCCCCTGCAATTCGATTACACCGGAAATGTCAGCAGAGGACTATGGCAGCCCGGTCGAGGGACCAAAACTTTCCGGCTAAGATATGGATCGAGTGTGCAGATTGTGCTGCAGGACACCAGCATTGTCACCACGGAAGATCACCCAATGCATCTCCATGGCTACCATTTCTATGTTGTGGGTTCGGGTTTTGGTAATTTCAATAGCGCGACGGACCCGGCGAGGTTCAATCTTGTTGACCCACCGATGAGGAACACCATTGGAACGCCGCCCGGTGGTTGGGTTGCGATTCGGTTCGTCGCTGATAATCCAGGAGCTTGGTTGATGCATTGCCATATCGACTCGCACCTTGCTTGGGGATTAGCCATGGTTTTTCTTGTCGAAAATGGCGAAGGGGAGATGCAATCTGTTCTTCCTCCTCCACCTGATCTCCCACCTTGTTAAATTTTATGCATTGTTCTTACTCCTTCATTATGTTTATGTATTGTTCTTGAATTAAGGAAAAAGCTTCG

mRNA sequence

ATGGAACATCTGTTAAAGACAAAATCTTTGTCTTCTTGCTTCTTGTTAGCCATTTTGGTCTTGATTCCTTTCGCCATTGCAGAAACTCACTACCATGAATTCATCGTTCAACGCAAACCAGTGAAGAGGCTGTGCAAAGTTCATAACATTATAACAGTGAACGGACAGTTCCCTGGGCCAATTTTAGAAGTAAGAAACGGCGATTCTTTGGTCATCAAAGTGGTTAACGCTGTCCGCTACAATGTCTCTCTTCATTGGCATGGGATCAAGCAGCTGAGGAACCCATGGGCGGATGGGCCGGAATTTATAACGCAATGCTCTATAAAGCCAGGGGGTAGCTATACTTACAGGTTCACTATTGAAGATCAAGAGGGTACTCTGTGGTGGCATGCGCATAGCCGATGGCTTCGAGCTACTGTTTATGGCGCGCTCATAATTTATCCGAAGATGGGTTCTTATTATCCGTTCTTAATGCCTAAAAGAGAAGCTTCTGTTCTTCTTGGGGAGTGGTTTGATAGAGATCCGATGAGTGTACTGCGGCAAGCGCTGTTGAGTGGAGCGGCTCCTAATGTGTCTGATGCTTACACCATCAACGGGCAGCCCGGCGATTTCTATAGATGCTCGAAGAAAGAGACGGTGAGATTCGGAGTGAATGCCGGCGAGACAATCCTTCTCAGAATCGTAAACTCCGGACTGAATCAAGAACTCTTCTTCTCCGTCGCGAACCACCCATTGACAGTGGTCGCCGTCGACGCATCTTACACCAAACCATTCAAAACAAATGTCATCATGATCGGACCCGGCCAAACCACCGACGTCCTCCTCACAGCCAACCAACCTCCAGCCCATTACTACATGGCCGCCACCGCTTACAACACCGCCATGAACGCCTCCTTCGACAACACCACCACCACTGCCATTCTCCAATACAACAACAATTACCAACAACAACCAAAACCCATCCTTGCCCAGCTCCCCTTTTTCAACGACACACCCACCTCCACCCGCTTCACCGCCCAACTCCGCAGCTTAAACAGAGCAAACGTCCCAACCCAAATCGACGAATCCTTACTCTTCACCGTCGGATTAGGCCTCATCAATTGCACCAACCCCAACAGCCCCCGCTGCCAAGGCCCCAACGGCACACGCTTCGCCGCCAGCATCAACAACAACTCCTTCGTTTTCCCCAAATCCAATTCCATTATGCAGGCCTTTTACCAAGGGATTCCCGGCGTTTTCACCACCGATTTCCCCCCTGTTCCGCCCCTGCAATTCGATTACACCGGAAATGTCAGCAGAGGACTATGGCAGCCCGGTCGAGGGACCAAAACTTTCCGGCTAAGATATGGATCGAGTGTGCAGATTGTGCTGCAGGACACCAGCATTGTCACCACGGAAGATCACCCAATGCATCTCCATGGCTACCATTTCTATGTTGTGGGTTCGGGTTTTGGTAATTTCAATAGCGCGACGGACCCGGCGAGGTTCAATCTTGTTGACCCACCGATGAGGAACACCATTGGAACGCCGCCCGGTGGTTGGGTTGCGATTCGGTTCGTCGCTGATAATCCAGGAGCTTGGTTGATGCATTGCCATATCGACTCGCACCTTGCTTGGGGATTAGCCATGGTTTTTCTTGTCGAAAATGGCGAAGGGGAGATGCAATCTGTTCTTCCTCCTCCACCTGATCTCCCACCTTGTTAAATTTTATGCATTGTTCTTACTCCTTCATTATGTTTATGTATTGTTCTTGAATTAAGGAAAAAGCTTCG

Coding sequence (CDS)

ATGGAACATCTGTTAAAGACAAAATCTTTGTCTTCTTGCTTCTTGTTAGCCATTTTGGTCTTGATTCCTTTCGCCATTGCAGAAACTCACTACCATGAATTCATCGTTCAACGCAAACCAGTGAAGAGGCTGTGCAAAGTTCATAACATTATAACAGTGAACGGACAGTTCCCTGGGCCAATTTTAGAAGTAAGAAACGGCGATTCTTTGGTCATCAAAGTGGTTAACGCTGTCCGCTACAATGTCTCTCTTCATTGGCATGGGATCAAGCAGCTGAGGAACCCATGGGCGGATGGGCCGGAATTTATAACGCAATGCTCTATAAAGCCAGGGGGTAGCTATACTTACAGGTTCACTATTGAAGATCAAGAGGGTACTCTGTGGTGGCATGCGCATAGCCGATGGCTTCGAGCTACTGTTTATGGCGCGCTCATAATTTATCCGAAGATGGGTTCTTATTATCCGTTCTTAATGCCTAAAAGAGAAGCTTCTGTTCTTCTTGGGGAGTGGTTTGATAGAGATCCGATGAGTGTACTGCGGCAAGCGCTGTTGAGTGGAGCGGCTCCTAATGTGTCTGATGCTTACACCATCAACGGGCAGCCCGGCGATTTCTATAGATGCTCGAAGAAAGAGACGGTGAGATTCGGAGTGAATGCCGGCGAGACAATCCTTCTCAGAATCGTAAACTCCGGACTGAATCAAGAACTCTTCTTCTCCGTCGCGAACCACCCATTGACAGTGGTCGCCGTCGACGCATCTTACACCAAACCATTCAAAACAAATGTCATCATGATCGGACCCGGCCAAACCACCGACGTCCTCCTCACAGCCAACCAACCTCCAGCCCATTACTACATGGCCGCCACCGCTTACAACACCGCCATGAACGCCTCCTTCGACAACACCACCACCACTGCCATTCTCCAATACAACAACAATTACCAACAACAACCAAAACCCATCCTTGCCCAGCTCCCCTTTTTCAACGACACACCCACCTCCACCCGCTTCACCGCCCAACTCCGCAGCTTAAACAGAGCAAACGTCCCAACCCAAATCGACGAATCCTTACTCTTCACCGTCGGATTAGGCCTCATCAATTGCACCAACCCCAACAGCCCCCGCTGCCAAGGCCCCAACGGCACACGCTTCGCCGCCAGCATCAACAACAACTCCTTCGTTTTCCCCAAATCCAATTCCATTATGCAGGCCTTTTACCAAGGGATTCCCGGCGTTTTCACCACCGATTTCCCCCCTGTTCCGCCCCTGCAATTCGATTACACCGGAAATGTCAGCAGAGGACTATGGCAGCCCGGTCGAGGGACCAAAACTTTCCGGCTAAGATATGGATCGAGTGTGCAGATTGTGCTGCAGGACACCAGCATTGTCACCACGGAAGATCACCCAATGCATCTCCATGGCTACCATTTCTATGTTGTGGGTTCGGGTTTTGGTAATTTCAATAGCGCGACGGACCCGGCGAGGTTCAATCTTGTTGACCCACCGATGAGGAACACCATTGGAACGCCGCCCGGTGGTTGGGTTGCGATTCGGTTCGTCGCTGATAATCCAGGAGCTTGGTTGATGCATTGCCATATCGACTCGCACCTTGCTTGGGGATTAGCCATGGTTTTTCTTGTCGAAAATGGCGAAGGGGAGATGCAATCTGTTCTTCCTCCTCCACCTGATCTCCCACCTTGTTAA

Protein sequence

MEHLLKTKSLSSCFLLAILVLIPFAIAETHYHEFIVQRKPVKRLCKVHNIITVNGQFPGPILEVRNGDSLVIKVVNAVRYNVSLHWHGIKQLRNPWADGPEFITQCSIKPGGSYTYRFTIEDQEGTLWWHAHSRWLRATVYGALIIYPKMGSYYPFLMPKREASVLLGEWFDRDPMSVLRQALLSGAAPNVSDAYTINGQPGDFYRCSKKETVRFGVNAGETILLRIVNSGLNQELFFSVANHPLTVVAVDASYTKPFKTNVIMIGPGQTTDVLLTANQPPAHYYMAATAYNTAMNASFDNTTTTAILQYNNNYQQQPKPILAQLPFFNDTPTSTRFTAQLRSLNRANVPTQIDESLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNNSFVFPKSNSIMQAFYQGIPGVFTTDFPPVPPLQFDYTGNVSRGLWQPGRGTKTFRLRYGSSVQIVLQDTSIVTTEDHPMHLHGYHFYVVGSGFGNFNSATDPARFNLVDPPMRNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSHLAWGLAMVFLVENGEGEMQSVLPPPPDLPPC
Homology
BLAST of CmoCh01G007320.1 vs. ExPASy Swiss-Prot
Match: Q56YT0 (Laccase-3 OS=Arabidopsis thaliana OX=3702 GN=LAC3 PE=2 SV=2)

HSP 1 Score: 877.1 bits (2265), Expect = 1.1e-253
Identity = 415/573 (72.43%), Postives = 469/573 (81.85%), Query Frame = 0

Query: 1   MEHLLKTKSLSSCFLLAILVLIPFAIAETHYHEFIVQRKPVKRLCKVHNIITVNGQFPGP 60
           ME   +   LS   LLA    +  A AE H H+F++   PVKRLC+ H  ITVNGQ+PGP
Sbjct: 1   MESFRRFSLLSFIALLAYFAFL--ASAEHHVHQFVITPTPVKRLCRTHQSITVNGQYPGP 60

Query: 61  ILEVRNGDSLVIKVVNAVRYNVSLHWHGIKQLRNPWADGPEFITQCSIKPGGSYTYRFTI 120
            L VRNGDSL I V+N  RYN+S+HWHGI+QLRNPWADGPE+ITQC I+PG +YTYRF I
Sbjct: 61  TLVVRNGDSLAITVINRARYNISIHWHGIRQLRNPWADGPEYITQCPIRPGQTYTYRFKI 120

Query: 121 EDQEGTLWWHAHSRWLRATVYGALIIYPKMGSYYPFLMPKREASVLLGEWFDRDPMSVLR 180
           EDQEGTLWWHAHSRWLRATVYGALIIYP++GS YPF MPKR+  +LLGEW+DR+PM VL+
Sbjct: 121 EDQEGTLWWHAHSRWLRATVYGALIIYPRLGSPYPFSMPKRDIPILLGEWWDRNPMDVLK 180

Query: 181 QALLSGAAPNVSDAYTINGQPGDFYRCSKKETVRFGVNAGETILLRIVNSGLNQELFFSV 240
           QA  +GAA NVSDAYTINGQPGD YRCS+  T+RF +  GET+ LR++N+G+NQELFFSV
Sbjct: 181 QAQFTGAAANVSDAYTINGQPGDLYRCSRAGTIRFPIFPGETVQLRVINAGMNQELFFSV 240

Query: 241 ANHPLTVVAVDASYTKPFKTNVIMIGPGQTTDVLLTANQPPAHYYMAATAYNTAMNASFD 300
           ANH  TVV  D++YTKPF TNVIMIGPGQTT+VLLTANQ P  YYMAA AYN+A NA FD
Sbjct: 241 ANHQFTVVETDSAYTKPFTTNVIMIGPGQTTNVLLTANQRPGRYYMAARAYNSA-NAPFD 300

Query: 301 NTTTTAILQYNN------NYQQQPKPILAQLPFFNDTPTSTRFTAQLRSLNRANVPTQID 360
           NTTTTAILQY N        + Q  P+   LP FNDT T+T FT +LR   RA VP Q+D
Sbjct: 301 NTTTTAILQYVNAPTRRGRGRGQIAPVFPVLPGFNDTATATAFTNRLRYWKRAPVPQQVD 360

Query: 361 ESLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNNSFVFPKSNSIMQAFYQGIPGVFT 420
           E+L FTVGLGLINC NPNSPRCQGPNGTRFAAS+NN SFV P+SNS+MQA+YQG PG+FT
Sbjct: 361 ENLFFTVGLGLINCANPNSPRCQGPNGTRFAASMNNMSFVLPRSNSVMQAYYQGTPGIFT 420

Query: 421 TDFPPVPPLQFDYTGNVSRGLWQPGRGTKTFRLRYGSSVQIVLQDTSIVTTEDHPMHLHG 480
           TDFPPVPP+QFDYTGNVSRGLWQP +GTK ++L+Y S+VQIVLQDTSIVT E+HPMHLHG
Sbjct: 421 TDFPPVPPVQFDYTGNVSRGLWQPIKGTKAYKLKYKSNVQIVLQDTSIVTPENHPMHLHG 480

Query: 481 YHFYVVGSGFGNFNSATDPARFNLVDPPMRNTIGTPPGGWVAIRFVADNPGAWLMHCHID 540
           Y FYVVGSGFGNFN  TDPARFNL DPP RNTIGTPPGGWVAIRFVADNPGAW MHCHID
Sbjct: 481 YQFYVVGSGFGNFNPRTDPARFNLFDPPERNTIGTPPGGWVAIRFVADNPGAWFMHCHID 540

Query: 541 SHLAWGLAMVFLVENGEGEMQSVLPPPPDLPPC 568
           SHL WGLAMVFLVENG G++QSV  PP DLP C
Sbjct: 541 SHLGWGLAMVFLVENGRGQLQSVQAPPLDLPRC 570

BLAST of CmoCh01G007320.1 vs. ExPASy Swiss-Prot
Match: Q9LYQ2 (Laccase-13 OS=Arabidopsis thaliana OX=3702 GN=LAC13 PE=2 SV=1)

HSP 1 Score: 875.9 bits (2262), Expect = 2.4e-253
Identity = 413/569 (72.58%), Postives = 474/569 (83.30%), Query Frame = 0

Query: 8   KSLSSCFLLAILVLIPFAIAETHYHEFIVQRKPVKRLCKVHNIITVNGQFPGPILEVRNG 67
           + L   FLL  + +     AE H+HEF++Q  PVKRLC+VHN ITVNGQFPGP LEVRNG
Sbjct: 2   EQLRPFFLLLAIFVASLVNAEVHFHEFVIQETPVKRLCRVHNSITVNGQFPGPTLEVRNG 61

Query: 68  DSLVIKVVNAVRYNVSLHWHGIKQLRNPWADGPEFITQCSIKPGGSYTYRFTIEDQEGTL 127
           DSLVI  +N  RYN+SLHWHGI+Q+RNPWADGPE+ITQC I+PGGSYTYRFT+EDQEGTL
Sbjct: 62  DSLVITAINKARYNISLHWHGIRQMRNPWADGPEYITQCPIQPGGSYTYRFTMEDQEGTL 121

Query: 128 WWHAHSRWLRATVYGALIIYPKMGS-YYPF-LMPKREASVLLGEWFDRDPMSVLRQALLS 187
           WWHAHSRWLRATVYGALII P + S +YPF ++PKRE ++LLGEW+DR+PM VL  A  +
Sbjct: 122 WWHAHSRWLRATVYGALIIRPPLSSPHYPFPVIPKREITLLLGEWWDRNPMDVLNLAQFT 181

Query: 188 GAAPNVSDAYTINGQPGDFYRCSKKETVRFGVNAGETILLRIVNSGLNQELFFSVANHPL 247
           GAAPN+SDA+TINGQPGD YRCS +ET+RF V +GE +LLR++NS LNQELFF VANH L
Sbjct: 182 GAAPNISDAFTINGQPGDLYRCSSQETLRFLVGSGEIVLLRVINSALNQELFFGVANHKL 241

Query: 248 TVVAVDASYTKPFKTNVIMIGPGQTTDVLLTANQPPAHYYMAATAYNTAMNASFDNTTTT 307
           TVVA DASYTKPF TNVIM+GPGQTTDVLLTA+QPPAHYYMAA AYN+A NA+FDNTTTT
Sbjct: 242 TVVAADASYTKPFSTNVIMLGPGQTTDVLLTADQPPAHYYMAAHAYNSA-NAAFDNTTTT 301

Query: 308 AILQYNN------NYQQQPKPILAQLPFFNDTPTSTRFTAQLRSLNRANVPTQIDESLLF 367
           AIL+Y +        + Q + I AQLP FNDT T+  FTAQ++S ++  VP +IDE+L F
Sbjct: 302 AILKYKDASCVTLQAKSQARAIPAQLPGFNDTATAAAFTAQMKSPSKVKVPLEIDENLFF 361

Query: 368 TVGLGLINCTNPNSPRCQGPNGTRFAASINNNSFVFPKSNSIMQAFYQGIP-GVFTTDFP 427
           TVGLGL NC  PN+ RCQGPNGTRF ASINN SFVFPK NSIMQA+YQG P GVFTTDFP
Sbjct: 362 TVGLGLFNCPTPNTQRCQGPNGTRFTASINNVSFVFPKQNSIMQAYYQGTPTGVFTTDFP 421

Query: 428 PVPPLQFDYTGNVSRGLWQPGRGTKTFRLRYGSSVQIVLQDTSIVTTEDHPMHLHGYHFY 487
           P PP+ FDYTGNVSRGLWQP RGTK ++L++ S VQI+LQDTSIVTTE+HPMHLHGY FY
Sbjct: 422 PTPPVTFDYTGNVSRGLWQPTRGTKAYKLKFNSQVQIILQDTSIVTTENHPMHLHGYEFY 481

Query: 488 VVGSGFGNFNSATDPARFNLVDPPMRNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSHLA 547
           VVG+G GNFN  TD + FNL+DPP RNTIGTPPGGWVAIRFVA+NPGAWLMHCHIDSH+ 
Sbjct: 482 VVGTGVGNFNPNTDTSSFNLIDPPRRNTIGTPPGGWVAIRFVANNPGAWLMHCHIDSHIF 541

Query: 548 WGLAMVFLVENGEGEMQSVLPPPPDLPPC 568
           WGLAMVFLVENGEG +QSV  PP DLP C
Sbjct: 542 WGLAMVFLVENGEGHLQSVQSPPLDLPQC 569

BLAST of CmoCh01G007320.1 vs. ExPASy Swiss-Prot
Match: Q9SIY8 (Laccase-5 OS=Arabidopsis thaliana OX=3702 GN=LAC5 PE=2 SV=1)

HSP 1 Score: 773.5 bits (1996), Expect = 1.7e-222
Identity = 359/578 (62.11%), Postives = 439/578 (75.95%), Query Frame = 0

Query: 7   TKSLSSCFLLAILVLIPFAIAE---THYHEFIVQRKPVKRLCKVHNIITVNGQFPGPILE 66
           TKSL  CF+  +  L+  ++AE    H+HEFI+Q   VKRLC+ HN ITVNG FPGP+L 
Sbjct: 4   TKSL-LCFISFVAFLLFSSVAEANKAHHHEFIIQATKVKRLCETHNSITVNGMFPGPMLV 63

Query: 67  VRNGDSLVIKVVNAVRYNVSLHWHGIKQLRNPWADGPEFITQCSIKPGGSYTYRFTIEDQ 126
           V NGD+LV+KV+N  RYN+++HWHG++Q+R  WADGPEF+TQC I+PG SYTYRFTI+ Q
Sbjct: 64  VNNGDTLVVKVINRARYNITIHWHGVRQMRTGWADGPEFVTQCPIRPGSSYTYRFTIQGQ 123

Query: 127 EGTLWWHAHSRWLRATVYGALIIYPKMGSYYPFLMPKREASVLLGEWFDRDPMSVLRQAL 186
           EGTLWWHAHS WLRATVYG+L+++P  GS YPF  P R   +LLGEW+D +P+ VLR+++
Sbjct: 124 EGTLWWHAHSSWLRATVYGSLLVFPPAGSSYPFTKPHRNVPLLLGEWWDANPVDVLRESI 183

Query: 187 LSGAAPNVSDAYTINGQPGDFYRCSKKETVRFGVNAGETILLRIVNSGLNQELFFSVANH 246
            +G APN SDAYTINGQPGD Y+CS ++T    +N GETILLR++NS LNQ LFF+VANH
Sbjct: 184 RTGGAPNNSDAYTINGQPGDLYKCSSQDTTVVPINVGETILLRVINSALNQPLFFTVANH 243

Query: 247 PLTVVAVDASYTKPFKTNVIMIGPGQTTDVLLTANQPPAHYYMAATAYNTAMNASFDNTT 306
            LTVV  DASY KPF TNVI++GPGQTTDVL+T +QPP  YYMAA AY +A NA F NTT
Sbjct: 244 KLTVVGADASYLKPFTTNVIVLGPGQTTDVLITGDQPPNRYYMAARAYQSAQNAPFGNTT 303

Query: 307 TTAILQYNN------------NYQQQPKPILAQLPFFNDTPTSTRFTAQLRSLNRANVPT 366
           TTAILQY +                  KPI+  LP +NDT T TRF+   RSL RA VPT
Sbjct: 304 TTAILQYKSAPCCGVGGGSGTKKGNSFKPIMPILPAYNDTNTVTRFSQSFRSLRRAEVPT 363

Query: 367 QIDESLLFTVGLGLINC-TNPNSPRCQGPNGTRFAASINNNSFVFPKSNSIMQAFYQGIP 426
           +IDE+L  T+GLGL NC  N  S RCQGPNGTRF AS+NN SF  P + S++QA + GIP
Sbjct: 364 EIDENLFVTIGLGLNNCPKNFRSRRCQGPNGTRFTASMNNVSFALPSNYSLLQAHHHGIP 423

Query: 427 GVFTTDFPPVPPLQFDYTG-NVSRGLWQPGRGTKTFRLRYGSSVQIVLQDTSIVTTEDHP 486
           GVFTTDFP  PP++FDYTG N+SR L+QP RGTK ++L+YGS VQIVLQDT IVT E+HP
Sbjct: 424 GVFTTDFPAKPPVKFDYTGNNISRSLYQPDRGTKLYKLKYGSRVQIVLQDTGIVTPENHP 483

Query: 487 MHLHGYHFYVVGSGFGNFNSATDPARFNLVDPPMRNTIGTPPGGWVAIRFVADNPGAWLM 546
           +HLHGY FY++  GFGNFN   D A+FNL DPP+RNT+G P  GW  IRF+ADNPG W+M
Sbjct: 484 IHLHGYDFYIIAEGFGNFNPKKDTAKFNLEDPPLRNTVGVPVNGWAVIRFIADNPGVWIM 543

Query: 547 HCHIDSHLAWGLAMVFLVENGEGEMQSVLPPPPDLPPC 568
           HCH+D+H++WGLAM FLVENG G +Q++  PP DLP C
Sbjct: 544 HCHLDAHISWGLAMAFLVENGNGVLQTIEQPPHDLPVC 580

BLAST of CmoCh01G007320.1 vs. ExPASy Swiss-Prot
Match: Q9FLB5 (Laccase-12 OS=Arabidopsis thaliana OX=3702 GN=LAC12 PE=2 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 7.3e-218
Identity = 349/545 (64.04%), Postives = 425/545 (77.98%), Query Frame = 0

Query: 26  IAETHYHEFIVQRKPVKRLCKVHNIITVNGQFPGPILEVRNGDSLVIKVVNAVRYNVSLH 85
           IA+  +H+F++Q  PVKRLCK  N ITVNG FPGP LEV NGD+L +KV N  RYN+++H
Sbjct: 23  IAKVQHHDFVIQETPVKRLCKTRNAITVNGMFPGPTLEVNNGDTLEVKVHNRARYNITIH 82

Query: 86  WHGIKQLRNPWADGPEFITQCSIKPGGSYTYRFTIEDQEGTLWWHAHSRWLRATVYGALI 145
           WHG++Q+R  WADGPEF+TQC I+PG SYTYRFTI+ QEGTLWWHAHS WLRATVYGALI
Sbjct: 83  WHGVRQIRTGWADGPEFVTQCPIRPGKSYTYRFTIQGQEGTLWWHAHSSWLRATVYGALI 142

Query: 146 IYPKMGSYYPFLMPKREASVLLGEWFDRDPMSVLRQALLSGAAPNVSDAYTINGQPGDFY 205
           I+P  GS +PF  P R+ +++LGEW++ +P+ V+ QA  +GAAPN+SDAYTINGQPGD Y
Sbjct: 143 IHPTPGSSFPFPKPDRQTALMLGEWWNANPVDVINQATRTGAAPNISDAYTINGQPGDLY 202

Query: 206 RCSKKETVRFGVNAGETILLRIVNSGLNQELFFSVANHPLTVVAVDASYTKPFKTNVIMI 265
            CS KETV   +N+GET LLR++N+ LNQ LFF+VANH LTVV  DASY KPF T V+M+
Sbjct: 203 NCSTKETVVVPINSGETSLLRVINAALNQPLFFTVANHKLTVVGADASYLKPFTTKVLML 262

Query: 266 GPGQTTDVLLTANQPPAHYYMAATAYNTAMNASFDNTTTTAILQYNNNYQQQPKPILAQL 325
           GPGQTTDVLLTA+QPP  YY+AA AY +A NA FDNTTTTAILQY        KPI+  L
Sbjct: 263 GPGQTTDVLLTADQPPKRYYIAARAYQSAQNAPFDNTTTTAILQYKKT-TTTSKPIMPVL 322

Query: 326 PFFNDTPTSTRFTAQLRSLNRANVPTQIDESLLFTVGLGLINCTN--PNSPRCQGPNGTR 385
           P FNDT T T F+ + +SL    VP  ID++L FT+GLGL NC    P S RCQG NGTR
Sbjct: 323 PAFNDTNTVTSFSRKFKSLRNVVVPKTIDDNLFFTIGLGLDNCPKKFPKS-RCQGLNGTR 382

Query: 386 FAASINNNSFVFPKSNSIMQAFYQGIPGVFTTDFPPVPPLQFDYTG-NVSRGLWQPGRGT 445
           F AS+NN SFV P + S++QA   GIPGVFTTDFP  PP++FDYTG N+SR L+QP +GT
Sbjct: 383 FTASMNNVSFVLPSNFSLLQAHSNGIPGVFTTDFPSKPPVKFDYTGNNISRALFQPVKGT 442

Query: 446 KTFRLRYGSSVQIVLQDTSIVTTEDHPMHLHGYHFYVVGSGFGNFNSATDPARFNLVDPP 505
           K ++L+YGS VQ+VLQDT+IVT+E+HP+HLHGY FY+VG GFGNFN   D ++FNLVDPP
Sbjct: 443 KLYKLKYGSRVQVVLQDTNIVTSENHPIHLHGYDFYIVGEGFGNFNPKKDTSKFNLVDPP 502

Query: 506 MRNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSHLAWGLAMVFLVENGEGEMQSVLPPPP 565
           +RNT+  P  GW  IRFVADNPG WLMHCH+D H+ WGLAM FLV+NG GE++++  PP 
Sbjct: 503 LRNTVAVPVNGWAVIRFVADNPGVWLMHCHLDVHIKWGLAMAFLVDNGVGELETLEAPPH 562

Query: 566 DLPPC 568
           DLP C
Sbjct: 563 DLPIC 565

BLAST of CmoCh01G007320.1 vs. ExPASy Swiss-Prot
Match: Q941X2 (Laccase-3 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC3 PE=2 SV=1)

HSP 1 Score: 745.0 bits (1922), Expect = 6.4e-214
Identity = 346/559 (61.90%), Postives = 419/559 (74.96%), Query Frame = 0

Query: 14  FLLAILVLIPFAIAETHYHEFIVQRKPVKRLCKVHNIITVNGQFPGPILEVRNGDSLVIK 73
           FLL+  VL   A AE H+HEFIVQ  PVKRLCK HN+ITVNGQ PGP LEVR GD++VI 
Sbjct: 11  FLLSCSVLALLAGAEVHHHEFIVQETPVKRLCKTHNVITVNGQLPGPTLEVREGDTVVIN 70

Query: 74  VVNAVRYNVSLHWHGIKQLRNPWADGPEFITQCSIKPGGSYTYRFTIEDQEGTLWWHAHS 133
           VVN  +YNV++HWHGI+Q R  WADGPEF+TQC IKPGGSY YRFTIE QEGTLWWHAHS
Sbjct: 71  VVNHAQYNVTIHWHGIRQFRTGWADGPEFVTQCPIKPGGSYKYRFTIEGQEGTLWWHAHS 130

Query: 134 RWLRATVYGALIIYPKMGSYYPFLMPKREASVLLGEWFDRDPMSVLRQALLSGAAPNVSD 193
            WLRATVYGALII P+    YPF  P RE  ++LGEW+D DP+ V+R+A  +GAAPN+SD
Sbjct: 131 SWLRATVYGALIIRPRENKTYPFEKPAREVPLILGEWWDADPIQVIREAQRTGAAPNISD 190

Query: 194 AYTINGQPGDFYRCSKKETVRFGVNAGETILLRIVNSGLNQELFFSVANHPLTVVAVDAS 253
           AYTINGQPGD Y CSK+ET    V  GET LLR +N+ LNQELF S+A H +TVV VDAS
Sbjct: 191 AYTINGQPGDLYNCSKEETTAVPVKPGETALLRFINAALNQELFVSIAQHKMTVVGVDAS 250

Query: 254 YTKPFKTNVIMIGPGQTTDVLLTANQPPAHYYMAATAYNTAMNASFDNTTTTAILQYN-- 313
           YTKPF T+V+MI PGQTTDVL+T +Q P  YY+AA AY++A   +FDNTTTTA+++Y+  
Sbjct: 251 YTKPFTTSVLMIAPGQTTDVLVTMDQAPTRYYLAARAYDSAQGVAFDNTTTTAVIEYDCG 310

Query: 314 --NNYQQQPKPILAQLPFFNDTPTSTRFTAQLRSLNRANVPTQIDESLLFTVGLGLINCT 373
              ++     P    LP FNDT T+T F A +RS +   +P  +DE+L FTVG+GL NC 
Sbjct: 311 CATDFGPSIPPAFPVLPAFNDTNTATAFAAGIRSPHEVKIPGPVDENLFFTVGVGLFNC- 370

Query: 374 NPNSPRCQGPNGTRFAASINNNSFVFPKSNSIMQAFYQGIPGVFTTDFPPVPPLQFDYTG 433
                +C GPN TRF AS+NN SFVFP++ S++ A Y GIPGVFTTDFP  PP+QFDYT 
Sbjct: 371 -EPGQQCGGPNNTRFTASMNNISFVFPQTTSLLHAHYYGIPGVFTTDFPAYPPVQFDYTA 430

Query: 434 -NVSRGLWQPGRGTKTFRLRYGSSVQIVLQDTSIVTTEDHPMHLHGYHFYVVGSGFGNFN 493
            NV R LWQP   TK ++L++GS VQIVLQDTSIV+ E+HP+H+HGY FY++  GFGNF+
Sbjct: 431 QNVPRYLWQPVPATKLYKLKFGSVVQIVLQDTSIVSPENHPIHIHGYDFYILAEGFGNFD 490

Query: 494 SATDPARFNLVDPPMRNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSHLAWGLAMVFLVE 553
              D  +FN VDPP RNT+  P  GW  IRFVADNPG WLMHCH+D H+ WGLAM FLVE
Sbjct: 491 PKKDAKKFNYVDPPQRNTVAVPTNGWAVIRFVADNPGVWLMHCHLDVHITWGLAMAFLVE 550

Query: 554 NGEGEMQSVLPPPPDLPPC 568
           +G G+++++  PP DLP C
Sbjct: 551 DGYGKLETLEAPPVDLPMC 567

BLAST of CmoCh01G007320.1 vs. ExPASy TrEMBL
Match: A0A6J1G957 (Laccase OS=Cucurbita moschata OX=3662 GN=LOC111452103 PE=3 SV=1)

HSP 1 Score: 1184.5 bits (3063), Expect = 0.0e+00
Identity = 567/567 (100.00%), Postives = 567/567 (100.00%), Query Frame = 0

Query: 1   MEHLLKTKSLSSCFLLAILVLIPFAIAETHYHEFIVQRKPVKRLCKVHNIITVNGQFPGP 60
           MEHLLKTKSLSSCFLLAILVLIPFAIAETHYHEFIVQRKPVKRLCKVHNIITVNGQFPGP
Sbjct: 1   MEHLLKTKSLSSCFLLAILVLIPFAIAETHYHEFIVQRKPVKRLCKVHNIITVNGQFPGP 60

Query: 61  ILEVRNGDSLVIKVVNAVRYNVSLHWHGIKQLRNPWADGPEFITQCSIKPGGSYTYRFTI 120
           ILEVRNGDSLVIKVVNAVRYNVSLHWHGIKQLRNPWADGPEFITQCSIKPGGSYTYRFTI
Sbjct: 61  ILEVRNGDSLVIKVVNAVRYNVSLHWHGIKQLRNPWADGPEFITQCSIKPGGSYTYRFTI 120

Query: 121 EDQEGTLWWHAHSRWLRATVYGALIIYPKMGSYYPFLMPKREASVLLGEWFDRDPMSVLR 180
           EDQEGTLWWHAHSRWLRATVYGALIIYPKMGSYYPFLMPKREASVLLGEWFDRDPMSVLR
Sbjct: 121 EDQEGTLWWHAHSRWLRATVYGALIIYPKMGSYYPFLMPKREASVLLGEWFDRDPMSVLR 180

Query: 181 QALLSGAAPNVSDAYTINGQPGDFYRCSKKETVRFGVNAGETILLRIVNSGLNQELFFSV 240
           QALLSGAAPNVSDAYTINGQPGDFYRCSKKETVRFGVNAGETILLRIVNSGLNQELFFSV
Sbjct: 181 QALLSGAAPNVSDAYTINGQPGDFYRCSKKETVRFGVNAGETILLRIVNSGLNQELFFSV 240

Query: 241 ANHPLTVVAVDASYTKPFKTNVIMIGPGQTTDVLLTANQPPAHYYMAATAYNTAMNASFD 300
           ANHPLTVVAVDASYTKPFKTNVIMIGPGQTTDVLLTANQPPAHYYMAATAYNTAMNASFD
Sbjct: 241 ANHPLTVVAVDASYTKPFKTNVIMIGPGQTTDVLLTANQPPAHYYMAATAYNTAMNASFD 300

Query: 301 NTTTTAILQYNNNYQQQPKPILAQLPFFNDTPTSTRFTAQLRSLNRANVPTQIDESLLFT 360
           NTTTTAILQYNNNYQQQPKPILAQLPFFNDTPTSTRFTAQLRSLNRANVPTQIDESLLFT
Sbjct: 301 NTTTTAILQYNNNYQQQPKPILAQLPFFNDTPTSTRFTAQLRSLNRANVPTQIDESLLFT 360

Query: 361 VGLGLINCTNPNSPRCQGPNGTRFAASINNNSFVFPKSNSIMQAFYQGIPGVFTTDFPPV 420
           VGLGLINCTNPNSPRCQGPNGTRFAASINNNSFVFPKSNSIMQAFYQGIPGVFTTDFPPV
Sbjct: 361 VGLGLINCTNPNSPRCQGPNGTRFAASINNNSFVFPKSNSIMQAFYQGIPGVFTTDFPPV 420

Query: 421 PPLQFDYTGNVSRGLWQPGRGTKTFRLRYGSSVQIVLQDTSIVTTEDHPMHLHGYHFYVV 480
           PPLQFDYTGNVSRGLWQPGRGTKTFRLRYGSSVQIVLQDTSIVTTEDHPMHLHGYHFYVV
Sbjct: 421 PPLQFDYTGNVSRGLWQPGRGTKTFRLRYGSSVQIVLQDTSIVTTEDHPMHLHGYHFYVV 480

Query: 481 GSGFGNFNSATDPARFNLVDPPMRNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSHLAWG 540
           GSGFGNFNSATDPARFNLVDPPMRNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSHLAWG
Sbjct: 481 GSGFGNFNSATDPARFNLVDPPMRNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSHLAWG 540

Query: 541 LAMVFLVENGEGEMQSVLPPPPDLPPC 568
           LAMVFLVENGEGEMQSVLPPPPDLPPC
Sbjct: 541 LAMVFLVENGEGEMQSVLPPPPDLPPC 567

BLAST of CmoCh01G007320.1 vs. ExPASy TrEMBL
Match: A0A6J1K8J5 (Laccase OS=Cucurbita maxima OX=3661 GN=LOC111493234 PE=3 SV=1)

HSP 1 Score: 1171.4 bits (3029), Expect = 0.0e+00
Identity = 557/567 (98.24%), Postives = 564/567 (99.47%), Query Frame = 0

Query: 1   MEHLLKTKSLSSCFLLAILVLIPFAIAETHYHEFIVQRKPVKRLCKVHNIITVNGQFPGP 60
           MEH+LKTKSLSSCFLLA+LVLIPFAIAETHYHEFIVQRKPVKRLCKVHNIITVNGQFPGP
Sbjct: 1   MEHMLKTKSLSSCFLLALLVLIPFAIAETHYHEFIVQRKPVKRLCKVHNIITVNGQFPGP 60

Query: 61  ILEVRNGDSLVIKVVNAVRYNVSLHWHGIKQLRNPWADGPEFITQCSIKPGGSYTYRFTI 120
            LEVRNGDSLVIKVVNAVRYNVSLHWHGIKQLRNPWADGPEF+TQCSIKPGGSYTYRFTI
Sbjct: 61  TLEVRNGDSLVIKVVNAVRYNVSLHWHGIKQLRNPWADGPEFVTQCSIKPGGSYTYRFTI 120

Query: 121 EDQEGTLWWHAHSRWLRATVYGALIIYPKMGSYYPFLMPKREASVLLGEWFDRDPMSVLR 180
           EDQEGTLWWHAHSRWLRATVYGALIIYPKMGSYYPFLMPKREASVLLGEWFDRDPMSVLR
Sbjct: 121 EDQEGTLWWHAHSRWLRATVYGALIIYPKMGSYYPFLMPKREASVLLGEWFDRDPMSVLR 180

Query: 181 QALLSGAAPNVSDAYTINGQPGDFYRCSKKETVRFGVNAGETILLRIVNSGLNQELFFSV 240
           QAL SGAAPNVSDAYTINGQPGDFYRCSKKETVRFGVN+GETILLRIVNSGLNQELFFSV
Sbjct: 181 QALFSGAAPNVSDAYTINGQPGDFYRCSKKETVRFGVNSGETILLRIVNSGLNQELFFSV 240

Query: 241 ANHPLTVVAVDASYTKPFKTNVIMIGPGQTTDVLLTANQPPAHYYMAATAYNTAMNASFD 300
           ANHPLTVVAVDASYTKPFKTNVIMIGPGQTTDVLLTANQPPAHYYMAATAYNTAMNA+FD
Sbjct: 241 ANHPLTVVAVDASYTKPFKTNVIMIGPGQTTDVLLTANQPPAHYYMAATAYNTAMNAAFD 300

Query: 301 NTTTTAILQYNNNYQQQPKPILAQLPFFNDTPTSTRFTAQLRSLNRANVPTQIDESLLFT 360
           NTTTTAILQYNNNYQQQ KPILAQLPFFNDTPTSTRFT+QLRSLNRANVPTQIDESLLFT
Sbjct: 301 NTTTTAILQYNNNYQQQQKPILAQLPFFNDTPTSTRFTSQLRSLNRANVPTQIDESLLFT 360

Query: 361 VGLGLINCTNPNSPRCQGPNGTRFAASINNNSFVFPKSNSIMQAFYQGIPGVFTTDFPPV 420
           VGLGLINCTNPNSPRCQGPNGTRFAASINNNSFVFPKSNSIMQAFYQGIPGVFTTDFPPV
Sbjct: 361 VGLGLINCTNPNSPRCQGPNGTRFAASINNNSFVFPKSNSIMQAFYQGIPGVFTTDFPPV 420

Query: 421 PPLQFDYTGNVSRGLWQPGRGTKTFRLRYGSSVQIVLQDTSIVTTEDHPMHLHGYHFYVV 480
           PPLQFDYTGNVSRGLWQPGRGTKTFRLRYGSSVQIVLQDTSIVTTEDHPMHLHGYHFYVV
Sbjct: 421 PPLQFDYTGNVSRGLWQPGRGTKTFRLRYGSSVQIVLQDTSIVTTEDHPMHLHGYHFYVV 480

Query: 481 GSGFGNFNSATDPARFNLVDPPMRNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSHLAWG 540
           GSGFGNFNSATDPARFNLVDPP+RNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSHLAWG
Sbjct: 481 GSGFGNFNSATDPARFNLVDPPLRNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSHLAWG 540

Query: 541 LAMVFLVENGEGEMQSVLPPPPDLPPC 568
           LAMVFLVENGEGEMQSVLPPPPDLPPC
Sbjct: 541 LAMVFLVENGEGEMQSVLPPPPDLPPC 567

BLAST of CmoCh01G007320.1 vs. ExPASy TrEMBL
Match: A0A6J1I651 (Laccase OS=Cucurbita maxima OX=3661 GN=LOC111469556 PE=3 SV=1)

HSP 1 Score: 1065.1 bits (2753), Expect = 1.0e-307
Identity = 507/574 (88.33%), Postives = 536/574 (93.38%), Query Frame = 0

Query: 1   MEHLLKTKSLSSCFLLA----ILVLIPFAIAETHYHEFIVQRKPVKRLCKVHNIITVNGQ 60
           ME LLK KS  S FLL+    +L+L PFA+AETHYHEFIVQ KPVKRLCKVHNIITVNG+
Sbjct: 1   MEELLKIKSFCSFFLLSLLAFLLLLPPFAVAETHYHEFIVQPKPVKRLCKVHNIITVNGK 60

Query: 61  FPGPILEVRNGDSLVIKVVNAVRYNVSLHWHGIKQLRNPWADGPEFITQCSIKPGGSYTY 120
           FPGP L VRNGDSLVIKVVN  RYNVSLHWHGI+QLRNPWADGPEF+TQCSIKPGGSYTY
Sbjct: 61  FPGPTLSVRNGDSLVIKVVNVARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSYTY 120

Query: 121 RFTIEDQEGTLWWHAHSRWLRATVYGALIIYPKMGSYYPFLMPKREASVLLGEWFDRDPM 180
           RFTI+DQEGTLWWHAHSRWLRATVYGALIIYPK+ S YPFLMPKREASVLLGEWFDRDP+
Sbjct: 121 RFTIQDQEGTLWWHAHSRWLRATVYGALIIYPKLPSSYPFLMPKREASVLLGEWFDRDPI 180

Query: 181 SVLRQALLSGAAPNVSDAYTINGQPGDFYRCSKKETVRFGVNAGETILLRIVNSGLNQEL 240
           SVLRQAL +GAAPNVSDAYTINGQPGDFYRCS KETVRF V++GETILLRI+NS LNQEL
Sbjct: 181 SVLRQALFTGAAPNVSDAYTINGQPGDFYRCSGKETVRFAVDSGETILLRIINSALNQEL 240

Query: 241 FFSVANHPLTVVAVDASYTKPFKTNVIMIGPGQTTDVLLTANQPPAHYYMAATAYNTAMN 300
           FFSVANH LTVVAVDASYTKPF TNVIMIGPGQTTDVLLTANQ PAHYYMAATAYNTA+N
Sbjct: 241 FFSVANHQLTVVAVDASYTKPFTTNVIMIGPGQTTDVLLTANQQPAHYYMAATAYNTALN 300

Query: 301 ASFDNTTTTAILQYNN---NYQQQPKPILAQLPFFNDTPTSTRFTAQLRSLNRANVPTQI 360
           A+FDNTTTTAIL+Y N   + QQ P+P +AQLP FNDTPT+TRFT+QLRS NR NVPTQI
Sbjct: 301 AAFDNTTTTAILEYKNCQQHQQQPPRPTMAQLPNFNDTPTATRFTSQLRSPNRVNVPTQI 360

Query: 361 DESLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNNSFVFPKSNSIMQAFYQGIPGVF 420
           D SLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINN SFVFPKSNSIMQAFYQG+PGVF
Sbjct: 361 DNSLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNVSFVFPKSNSIMQAFYQGVPGVF 420

Query: 421 TTDFPPVPPLQFDYTGNVSRGLWQPGRGTKTFRLRYGSSVQIVLQDTSIVTTEDHPMHLH 480
           TTDFPPVPPLQFDYTGNVSRGLWQPGRGTK +RL+YGS+VQIVLQDTSIVTTEDHPMHLH
Sbjct: 421 TTDFPPVPPLQFDYTGNVSRGLWQPGRGTKGYRLKYGSNVQIVLQDTSIVTTEDHPMHLH 480

Query: 481 GYHFYVVGSGFGNFNSATDPARFNLVDPPMRNTIGTPPGGWVAIRFVADNPGAWLMHCHI 540
           GYHFYVVGSGFGNFN ATDPARFNL+DPP+RNTIGTPPGGWVAIRFVADNPGAWLMHCHI
Sbjct: 481 GYHFYVVGSGFGNFNPATDPARFNLIDPPVRNTIGTPPGGWVAIRFVADNPGAWLMHCHI 540

Query: 541 DSHLAWGLAMVFLVENGEGEMQSVLPPPPDLPPC 568
           DSHLAWGLAMVFLVENGEGE+QSVLPPPPDLPPC
Sbjct: 541 DSHLAWGLAMVFLVENGEGELQSVLPPPPDLPPC 574

BLAST of CmoCh01G007320.1 vs. ExPASy TrEMBL
Match: A0A6J1HPK1 (Laccase OS=Cucurbita moschata OX=3662 GN=LOC111464877 PE=3 SV=1)

HSP 1 Score: 1061.6 bits (2744), Expect = 1.1e-306
Identity = 504/574 (87.80%), Postives = 537/574 (93.55%), Query Frame = 0

Query: 1   MEHLLKTKSLSSCFLLA----ILVLIPFAIAETHYHEFIVQRKPVKRLCKVHNIITVNGQ 60
           ME LLK KS  S FLL+    +L+L PFA+AETHYHEFIVQ KPVKRLCKVHNIITVNG+
Sbjct: 1   MEELLKIKSCCSFFLLSLLAFLLLLPPFAVAETHYHEFIVQPKPVKRLCKVHNIITVNGK 60

Query: 61  FPGPILEVRNGDSLVIKVVNAVRYNVSLHWHGIKQLRNPWADGPEFITQCSIKPGGSYTY 120
           FPGP L VRNGDSLVIKVVN  RYNVSLHWHGI+QLRNPWADGPEF+TQCSIKPGGSYTY
Sbjct: 61  FPGPTLAVRNGDSLVIKVVNVARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSYTY 120

Query: 121 RFTIEDQEGTLWWHAHSRWLRATVYGALIIYPKMGSYYPFLMPKREASVLLGEWFDRDPM 180
           RFTI+DQEGTLWWHAHSRWLRATVYGALIIYPK+ S YP LMPKREASVLLGEWFDRDP+
Sbjct: 121 RFTIQDQEGTLWWHAHSRWLRATVYGALIIYPKLPSSYPSLMPKREASVLLGEWFDRDPI 180

Query: 181 SVLRQALLSGAAPNVSDAYTINGQPGDFYRCSKKETVRFGVNAGETILLRIVNSGLNQEL 240
           SVLRQAL +GAAPNVSDAYTINGQPGDFYRCS KETVRF V++GETILLRI+NS LNQEL
Sbjct: 181 SVLRQALFTGAAPNVSDAYTINGQPGDFYRCSGKETVRFAVDSGETILLRIINSALNQEL 240

Query: 241 FFSVANHPLTVVAVDASYTKPFKTNVIMIGPGQTTDVLLTANQPPAHYYMAATAYNTAMN 300
           FFSVANH LTV+AVDASYTKPF TNVIM+GPGQTTDVLLTANQ PAHYYMAATAYNTA+N
Sbjct: 241 FFSVANHQLTVLAVDASYTKPFTTNVIMVGPGQTTDVLLTANQQPAHYYMAATAYNTALN 300

Query: 301 ASFDNTTTTAILQYNN---NYQQQPKPILAQLPFFNDTPTSTRFTAQLRSLNRANVPTQI 360
           A+FDNTTTTAIL+Y N   + QQ P+PI+AQLP FNDTPT+TRFT+QLRS NR NVPT+I
Sbjct: 301 AAFDNTTTTAILEYKNCQQHQQQPPRPIMAQLPNFNDTPTATRFTSQLRSPNRVNVPTRI 360

Query: 361 DESLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNNSFVFPKSNSIMQAFYQGIPGVF 420
           D+SLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINN SFVFPKSNSIMQAFYQG+PGVF
Sbjct: 361 DDSLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNVSFVFPKSNSIMQAFYQGVPGVF 420

Query: 421 TTDFPPVPPLQFDYTGNVSRGLWQPGRGTKTFRLRYGSSVQIVLQDTSIVTTEDHPMHLH 480
           TTDFPPVPPLQFDYTGNVSRGLWQPGRGTK +RL+YGS+VQIVLQDTSIVTTEDHPMHLH
Sbjct: 421 TTDFPPVPPLQFDYTGNVSRGLWQPGRGTKAYRLKYGSNVQIVLQDTSIVTTEDHPMHLH 480

Query: 481 GYHFYVVGSGFGNFNSATDPARFNLVDPPMRNTIGTPPGGWVAIRFVADNPGAWLMHCHI 540
           GYHFYVVGSGFGNFN ATDPARFNL+DPP+RNTIGTPPGGWVAIRFVADNPGAWLMHCHI
Sbjct: 481 GYHFYVVGSGFGNFNPATDPARFNLIDPPVRNTIGTPPGGWVAIRFVADNPGAWLMHCHI 540

Query: 541 DSHLAWGLAMVFLVENGEGEMQSVLPPPPDLPPC 568
           DSHLAWGLAMVFLVENGEGE+QSVLPPPPDLPPC
Sbjct: 541 DSHLAWGLAMVFLVENGEGELQSVLPPPPDLPPC 574

BLAST of CmoCh01G007320.1 vs. ExPASy TrEMBL
Match: A0A5D3BDS8 (Laccase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G00710 PE=3 SV=1)

HSP 1 Score: 1003.0 bits (2592), Expect = 4.8e-289
Identity = 478/574 (83.28%), Postives = 516/574 (89.90%), Query Frame = 0

Query: 1   MEHLLKTK-SLSSCFLLAIL-----VLIPFAIAETHYHEFIVQRKPVKRLCKVHNIITVN 60
           ME  +  K S+ S FLL  L     +L  F + +THYH+FIVQ KPVKRLCKVHNIITVN
Sbjct: 1   MEEFVNLKSSICSFFLLISLLGFLPLLSAFPLPKTHYHQFIVQPKPVKRLCKVHNIITVN 60

Query: 61  GQFPGPILEVRNGDSLVIKVVNAVRYNVSLHWHGIKQLRNPWADGPEFITQCSIKPGGSY 120
           GQFPGP L VR+GDSLVIKVVNA RYNVSLHWHGI+QLRNPWADGPEFITQCSIKPGGSY
Sbjct: 61  GQFPGPTLAVRDGDSLVIKVVNAARYNVSLHWHGIRQLRNPWADGPEFITQCSIKPGGSY 120

Query: 121 TYRFTIEDQEGTLWWHAHSRWLRATVYGALIIYPKMGSYYPFLMPKREASVLLGEWFDRD 180
           TYRFTIE QEGTLWWHAHSRWLRATVYGALIIYPK+ S +PF+MPK+E  +LLGEWFDRD
Sbjct: 121 TYRFTIEGQEGTLWWHAHSRWLRATVYGALIIYPKLASPHPFVMPKKEFPLLLGEWFDRD 180

Query: 181 PMSVLRQALLSGAAPNVSDAYTINGQPGDFYRCSKKETVRFGVNAGETILLRIVNSGLNQ 240
           P+SVLRQAL +GAAPNVSDAYTINGQPGDFY CSKKET+R  VN+GETILLRI+NS LNQ
Sbjct: 181 PISVLRQALFTGAAPNVSDAYTINGQPGDFYICSKKETMRLAVNSGETILLRIINSALNQ 240

Query: 241 ELFFSVANHPLTVVAVDASYTKPFKTNVIMIGPGQTTDVLLTANQPPAHYYMAATAYNTA 300
           ELFFS+ANH +TVVAVDA+YTKPF TNVIMIGPGQTTDVL+TANQPPA+YYMAATAYNTA
Sbjct: 241 ELFFSIANHQMTVVAVDAAYTKPFATNVIMIGPGQTTDVLVTANQPPAYYYMAATAYNTA 300

Query: 301 MNASFDNTTTTAILQYNN-NYQQQPKPILAQLPFFNDTPTSTRFTAQLRSLNRANVPTQI 360
            NA FDNTTTTAILQYNN   QQ P+PILAQLP FNDTPT+TRFT QLRS +R +VP  I
Sbjct: 301 QNAPFDNTTTTAILQYNNLPPQQNPRPILAQLPNFNDTPTATRFTDQLRSPHRVSVPLHI 360

Query: 361 DESLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNNSFVFPKSNSIMQAFYQGIPGVF 420
           DE+L FTVGLGL NCTNPNSPRCQGPNGTRF ASINN SFVFPKSNSIMQA+YQG+PGVF
Sbjct: 361 DENLFFTVGLGLNNCTNPNSPRCQGPNGTRFTASINNVSFVFPKSNSIMQAYYQGVPGVF 420

Query: 421 TTDFPPVPPLQFDYTGNVSRGLWQPGRGTKTFRLRYGSSVQIVLQDTSIVTTEDHPMHLH 480
           T DFPP PPLQFDYTGNVSRGLWQP RGTK ++L+YGSSVQIVLQDTSIVT E+HPMHLH
Sbjct: 421 TADFPPFPPLQFDYTGNVSRGLWQPRRGTKAYKLKYGSSVQIVLQDTSIVTPENHPMHLH 480

Query: 481 GYHFYVVGSGFGNFNSATDPARFNLVDPPMRNTIGTPPGGWVAIRFVADNPGAWLMHCHI 540
           GYHFYVVGSGFGNFN  TDPARFNL+DPP+RNTIGTP GGWVAIRF+ADNPGAWLMHCHI
Sbjct: 481 GYHFYVVGSGFGNFNPRTDPARFNLIDPPVRNTIGTPTGGWVAIRFIADNPGAWLMHCHI 540

Query: 541 DSHLAWGLAMVFLVENGEGEMQSVLPPPPDLPPC 568
           DSHLAWGLAMVFLVENGEGEMQSV+PPPPDLPPC
Sbjct: 541 DSHLAWGLAMVFLVENGEGEMQSVIPPPPDLPPC 574

BLAST of CmoCh01G007320.1 vs. NCBI nr
Match: XP_022948412.1 (laccase-3 [Cucurbita moschata])

HSP 1 Score: 1184.5 bits (3063), Expect = 0.0e+00
Identity = 567/567 (100.00%), Postives = 567/567 (100.00%), Query Frame = 0

Query: 1   MEHLLKTKSLSSCFLLAILVLIPFAIAETHYHEFIVQRKPVKRLCKVHNIITVNGQFPGP 60
           MEHLLKTKSLSSCFLLAILVLIPFAIAETHYHEFIVQRKPVKRLCKVHNIITVNGQFPGP
Sbjct: 1   MEHLLKTKSLSSCFLLAILVLIPFAIAETHYHEFIVQRKPVKRLCKVHNIITVNGQFPGP 60

Query: 61  ILEVRNGDSLVIKVVNAVRYNVSLHWHGIKQLRNPWADGPEFITQCSIKPGGSYTYRFTI 120
           ILEVRNGDSLVIKVVNAVRYNVSLHWHGIKQLRNPWADGPEFITQCSIKPGGSYTYRFTI
Sbjct: 61  ILEVRNGDSLVIKVVNAVRYNVSLHWHGIKQLRNPWADGPEFITQCSIKPGGSYTYRFTI 120

Query: 121 EDQEGTLWWHAHSRWLRATVYGALIIYPKMGSYYPFLMPKREASVLLGEWFDRDPMSVLR 180
           EDQEGTLWWHAHSRWLRATVYGALIIYPKMGSYYPFLMPKREASVLLGEWFDRDPMSVLR
Sbjct: 121 EDQEGTLWWHAHSRWLRATVYGALIIYPKMGSYYPFLMPKREASVLLGEWFDRDPMSVLR 180

Query: 181 QALLSGAAPNVSDAYTINGQPGDFYRCSKKETVRFGVNAGETILLRIVNSGLNQELFFSV 240
           QALLSGAAPNVSDAYTINGQPGDFYRCSKKETVRFGVNAGETILLRIVNSGLNQELFFSV
Sbjct: 181 QALLSGAAPNVSDAYTINGQPGDFYRCSKKETVRFGVNAGETILLRIVNSGLNQELFFSV 240

Query: 241 ANHPLTVVAVDASYTKPFKTNVIMIGPGQTTDVLLTANQPPAHYYMAATAYNTAMNASFD 300
           ANHPLTVVAVDASYTKPFKTNVIMIGPGQTTDVLLTANQPPAHYYMAATAYNTAMNASFD
Sbjct: 241 ANHPLTVVAVDASYTKPFKTNVIMIGPGQTTDVLLTANQPPAHYYMAATAYNTAMNASFD 300

Query: 301 NTTTTAILQYNNNYQQQPKPILAQLPFFNDTPTSTRFTAQLRSLNRANVPTQIDESLLFT 360
           NTTTTAILQYNNNYQQQPKPILAQLPFFNDTPTSTRFTAQLRSLNRANVPTQIDESLLFT
Sbjct: 301 NTTTTAILQYNNNYQQQPKPILAQLPFFNDTPTSTRFTAQLRSLNRANVPTQIDESLLFT 360

Query: 361 VGLGLINCTNPNSPRCQGPNGTRFAASINNNSFVFPKSNSIMQAFYQGIPGVFTTDFPPV 420
           VGLGLINCTNPNSPRCQGPNGTRFAASINNNSFVFPKSNSIMQAFYQGIPGVFTTDFPPV
Sbjct: 361 VGLGLINCTNPNSPRCQGPNGTRFAASINNNSFVFPKSNSIMQAFYQGIPGVFTTDFPPV 420

Query: 421 PPLQFDYTGNVSRGLWQPGRGTKTFRLRYGSSVQIVLQDTSIVTTEDHPMHLHGYHFYVV 480
           PPLQFDYTGNVSRGLWQPGRGTKTFRLRYGSSVQIVLQDTSIVTTEDHPMHLHGYHFYVV
Sbjct: 421 PPLQFDYTGNVSRGLWQPGRGTKTFRLRYGSSVQIVLQDTSIVTTEDHPMHLHGYHFYVV 480

Query: 481 GSGFGNFNSATDPARFNLVDPPMRNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSHLAWG 540
           GSGFGNFNSATDPARFNLVDPPMRNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSHLAWG
Sbjct: 481 GSGFGNFNSATDPARFNLVDPPMRNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSHLAWG 540

Query: 541 LAMVFLVENGEGEMQSVLPPPPDLPPC 568
           LAMVFLVENGEGEMQSVLPPPPDLPPC
Sbjct: 541 LAMVFLVENGEGEMQSVLPPPPDLPPC 567

BLAST of CmoCh01G007320.1 vs. NCBI nr
Match: KAG7037020.1 (Laccase-3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1178.3 bits (3047), Expect = 0.0e+00
Identity = 562/567 (99.12%), Postives = 566/567 (99.82%), Query Frame = 0

Query: 1   MEHLLKTKSLSSCFLLAILVLIPFAIAETHYHEFIVQRKPVKRLCKVHNIITVNGQFPGP 60
           MEHLLKTKSLSSCFLLA+LVLIPFAIAETHYHEFIVQRKPVKRLCKVHNIITVNGQFPGP
Sbjct: 1   MEHLLKTKSLSSCFLLALLVLIPFAIAETHYHEFIVQRKPVKRLCKVHNIITVNGQFPGP 60

Query: 61  ILEVRNGDSLVIKVVNAVRYNVSLHWHGIKQLRNPWADGPEFITQCSIKPGGSYTYRFTI 120
            LEVRNGDSLVIKVVNAVRYNVSLHWHGIKQLRNPWADGPEFITQCSIKPGGSYTYRFTI
Sbjct: 61  TLEVRNGDSLVIKVVNAVRYNVSLHWHGIKQLRNPWADGPEFITQCSIKPGGSYTYRFTI 120

Query: 121 EDQEGTLWWHAHSRWLRATVYGALIIYPKMGSYYPFLMPKREASVLLGEWFDRDPMSVLR 180
           EDQEGTLWWHAHSRWLRATVYGALIIYPKMGSYYPFLMPKREASVLLGEWFDRDPMSVLR
Sbjct: 121 EDQEGTLWWHAHSRWLRATVYGALIIYPKMGSYYPFLMPKREASVLLGEWFDRDPMSVLR 180

Query: 181 QALLSGAAPNVSDAYTINGQPGDFYRCSKKETVRFGVNAGETILLRIVNSGLNQELFFSV 240
           QALLSGAAPNVSDAYTINGQPGDFYRCSKKETVRFGVN+GETILLRIVNSGLNQELFFSV
Sbjct: 181 QALLSGAAPNVSDAYTINGQPGDFYRCSKKETVRFGVNSGETILLRIVNSGLNQELFFSV 240

Query: 241 ANHPLTVVAVDASYTKPFKTNVIMIGPGQTTDVLLTANQPPAHYYMAATAYNTAMNASFD 300
           ANHPLTVVAVDASYTKPFKTNVIMIGPGQTTDVLLTANQPPAHYYMAATAYNTAMNA+FD
Sbjct: 241 ANHPLTVVAVDASYTKPFKTNVIMIGPGQTTDVLLTANQPPAHYYMAATAYNTAMNAAFD 300

Query: 301 NTTTTAILQYNNNYQQQPKPILAQLPFFNDTPTSTRFTAQLRSLNRANVPTQIDESLLFT 360
           NTTTTAILQYNNNYQQQPKPILAQLPFFNDTPTSTRFTAQLRSLNRANVPTQIDESLLFT
Sbjct: 301 NTTTTAILQYNNNYQQQPKPILAQLPFFNDTPTSTRFTAQLRSLNRANVPTQIDESLLFT 360

Query: 361 VGLGLINCTNPNSPRCQGPNGTRFAASINNNSFVFPKSNSIMQAFYQGIPGVFTTDFPPV 420
           VGLGLINCTNPNSPRCQGPNGTRFAASINNNSFVFPKSNSIMQAFYQGIPGVFTTDFPPV
Sbjct: 361 VGLGLINCTNPNSPRCQGPNGTRFAASINNNSFVFPKSNSIMQAFYQGIPGVFTTDFPPV 420

Query: 421 PPLQFDYTGNVSRGLWQPGRGTKTFRLRYGSSVQIVLQDTSIVTTEDHPMHLHGYHFYVV 480
           PPLQFDYTGNVSRGLWQPGRGTKTFRLRYGSSVQIVLQDTSIVTTEDHPMHLHGYHFYVV
Sbjct: 421 PPLQFDYTGNVSRGLWQPGRGTKTFRLRYGSSVQIVLQDTSIVTTEDHPMHLHGYHFYVV 480

Query: 481 GSGFGNFNSATDPARFNLVDPPMRNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSHLAWG 540
           GSGFGNFNSATDPARFNLVDPP+RNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSHLAWG
Sbjct: 481 GSGFGNFNSATDPARFNLVDPPLRNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSHLAWG 540

Query: 541 LAMVFLVENGEGEMQSVLPPPPDLPPC 568
           LAMVFLVENGEGEMQSVLPPPPDLPPC
Sbjct: 541 LAMVFLVENGEGEMQSVLPPPPDLPPC 567

BLAST of CmoCh01G007320.1 vs. NCBI nr
Match: XP_022998647.1 (laccase-3 [Cucurbita maxima])

HSP 1 Score: 1171.4 bits (3029), Expect = 0.0e+00
Identity = 557/567 (98.24%), Postives = 564/567 (99.47%), Query Frame = 0

Query: 1   MEHLLKTKSLSSCFLLAILVLIPFAIAETHYHEFIVQRKPVKRLCKVHNIITVNGQFPGP 60
           MEH+LKTKSLSSCFLLA+LVLIPFAIAETHYHEFIVQRKPVKRLCKVHNIITVNGQFPGP
Sbjct: 1   MEHMLKTKSLSSCFLLALLVLIPFAIAETHYHEFIVQRKPVKRLCKVHNIITVNGQFPGP 60

Query: 61  ILEVRNGDSLVIKVVNAVRYNVSLHWHGIKQLRNPWADGPEFITQCSIKPGGSYTYRFTI 120
            LEVRNGDSLVIKVVNAVRYNVSLHWHGIKQLRNPWADGPEF+TQCSIKPGGSYTYRFTI
Sbjct: 61  TLEVRNGDSLVIKVVNAVRYNVSLHWHGIKQLRNPWADGPEFVTQCSIKPGGSYTYRFTI 120

Query: 121 EDQEGTLWWHAHSRWLRATVYGALIIYPKMGSYYPFLMPKREASVLLGEWFDRDPMSVLR 180
           EDQEGTLWWHAHSRWLRATVYGALIIYPKMGSYYPFLMPKREASVLLGEWFDRDPMSVLR
Sbjct: 121 EDQEGTLWWHAHSRWLRATVYGALIIYPKMGSYYPFLMPKREASVLLGEWFDRDPMSVLR 180

Query: 181 QALLSGAAPNVSDAYTINGQPGDFYRCSKKETVRFGVNAGETILLRIVNSGLNQELFFSV 240
           QAL SGAAPNVSDAYTINGQPGDFYRCSKKETVRFGVN+GETILLRIVNSGLNQELFFSV
Sbjct: 181 QALFSGAAPNVSDAYTINGQPGDFYRCSKKETVRFGVNSGETILLRIVNSGLNQELFFSV 240

Query: 241 ANHPLTVVAVDASYTKPFKTNVIMIGPGQTTDVLLTANQPPAHYYMAATAYNTAMNASFD 300
           ANHPLTVVAVDASYTKPFKTNVIMIGPGQTTDVLLTANQPPAHYYMAATAYNTAMNA+FD
Sbjct: 241 ANHPLTVVAVDASYTKPFKTNVIMIGPGQTTDVLLTANQPPAHYYMAATAYNTAMNAAFD 300

Query: 301 NTTTTAILQYNNNYQQQPKPILAQLPFFNDTPTSTRFTAQLRSLNRANVPTQIDESLLFT 360
           NTTTTAILQYNNNYQQQ KPILAQLPFFNDTPTSTRFT+QLRSLNRANVPTQIDESLLFT
Sbjct: 301 NTTTTAILQYNNNYQQQQKPILAQLPFFNDTPTSTRFTSQLRSLNRANVPTQIDESLLFT 360

Query: 361 VGLGLINCTNPNSPRCQGPNGTRFAASINNNSFVFPKSNSIMQAFYQGIPGVFTTDFPPV 420
           VGLGLINCTNPNSPRCQGPNGTRFAASINNNSFVFPKSNSIMQAFYQGIPGVFTTDFPPV
Sbjct: 361 VGLGLINCTNPNSPRCQGPNGTRFAASINNNSFVFPKSNSIMQAFYQGIPGVFTTDFPPV 420

Query: 421 PPLQFDYTGNVSRGLWQPGRGTKTFRLRYGSSVQIVLQDTSIVTTEDHPMHLHGYHFYVV 480
           PPLQFDYTGNVSRGLWQPGRGTKTFRLRYGSSVQIVLQDTSIVTTEDHPMHLHGYHFYVV
Sbjct: 421 PPLQFDYTGNVSRGLWQPGRGTKTFRLRYGSSVQIVLQDTSIVTTEDHPMHLHGYHFYVV 480

Query: 481 GSGFGNFNSATDPARFNLVDPPMRNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSHLAWG 540
           GSGFGNFNSATDPARFNLVDPP+RNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSHLAWG
Sbjct: 481 GSGFGNFNSATDPARFNLVDPPLRNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSHLAWG 540

Query: 541 LAMVFLVENGEGEMQSVLPPPPDLPPC 568
           LAMVFLVENGEGEMQSVLPPPPDLPPC
Sbjct: 541 LAMVFLVENGEGEMQSVLPPPPDLPPC 567

BLAST of CmoCh01G007320.1 vs. NCBI nr
Match: XP_023525776.1 (laccase-13-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1165.2 bits (3013), Expect = 0.0e+00
Identity = 557/567 (98.24%), Postives = 561/567 (98.94%), Query Frame = 0

Query: 1   MEHLLKTKSLSSCFLLAILVLIPFAIAETHYHEFIVQRKPVKRLCKVHNIITVNGQFPGP 60
           MEHLLKTKSLSSCFLLA+LVLIPFAIAETHYHEFIVQRKPVKRLCKVHNIITVN QFPGP
Sbjct: 1   MEHLLKTKSLSSCFLLALLVLIPFAIAETHYHEFIVQRKPVKRLCKVHNIITVNRQFPGP 60

Query: 61  ILEVRNGDSLVIKVVNAVRYNVSLHWHGIKQLRNPWADGPEFITQCSIKPGGSYTYRFTI 120
            LEVRNGDSLVIKVVNAVRYNVSLHWHGIKQLRNPWADGPEFITQCSIKPGGSYTYRFTI
Sbjct: 61  TLEVRNGDSLVIKVVNAVRYNVSLHWHGIKQLRNPWADGPEFITQCSIKPGGSYTYRFTI 120

Query: 121 EDQEGTLWWHAHSRWLRATVYGALIIYPKMGSYYPFLMPKREASVLLGEWFDRDPMSVLR 180
           EDQEGTLWWHAHSRWLRATVYGALIIYPKMGSYYPFLMPKREASVLLGEWFDRDPMSVLR
Sbjct: 121 EDQEGTLWWHAHSRWLRATVYGALIIYPKMGSYYPFLMPKREASVLLGEWFDRDPMSVLR 180

Query: 181 QALLSGAAPNVSDAYTINGQPGDFYRCSKKETVRFGVNAGETILLRIVNSGLNQELFFSV 240
           QALLSGAAPNVSDAYTINGQPGDFY CSKKETVR  VN+GETILLRIVNSGLNQELFFSV
Sbjct: 181 QALLSGAAPNVSDAYTINGQPGDFYSCSKKETVRIRVNSGETILLRIVNSGLNQELFFSV 240

Query: 241 ANHPLTVVAVDASYTKPFKTNVIMIGPGQTTDVLLTANQPPAHYYMAATAYNTAMNASFD 300
           ANHPLTVVAVDASYTKPFKTNVIMIGPGQTTDVLLTANQPPAHYYMAATAYNTAMNA+FD
Sbjct: 241 ANHPLTVVAVDASYTKPFKTNVIMIGPGQTTDVLLTANQPPAHYYMAATAYNTAMNAAFD 300

Query: 301 NTTTTAILQYNNNYQQQPKPILAQLPFFNDTPTSTRFTAQLRSLNRANVPTQIDESLLFT 360
           NTTTTAILQYN NYQQQPKPILAQLPFFNDTPTSTRFTAQLRSLNRANVPTQIDESLLFT
Sbjct: 301 NTTTTAILQYNKNYQQQPKPILAQLPFFNDTPTSTRFTAQLRSLNRANVPTQIDESLLFT 360

Query: 361 VGLGLINCTNPNSPRCQGPNGTRFAASINNNSFVFPKSNSIMQAFYQGIPGVFTTDFPPV 420
           VGLGLINCTNPNSPRCQGPNGTRFAASINNNSFVFPKSNSIMQAFYQGIPGVFTTDFPPV
Sbjct: 361 VGLGLINCTNPNSPRCQGPNGTRFAASINNNSFVFPKSNSIMQAFYQGIPGVFTTDFPPV 420

Query: 421 PPLQFDYTGNVSRGLWQPGRGTKTFRLRYGSSVQIVLQDTSIVTTEDHPMHLHGYHFYVV 480
           PPLQFDYTGNVSRGLWQPGRGTKTFRLRYGSSVQIVLQDTSIVTTEDHPMHLHGYHFYVV
Sbjct: 421 PPLQFDYTGNVSRGLWQPGRGTKTFRLRYGSSVQIVLQDTSIVTTEDHPMHLHGYHFYVV 480

Query: 481 GSGFGNFNSATDPARFNLVDPPMRNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSHLAWG 540
           GSGFGNFNSATDPARFNLVDPP+RNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSHLAWG
Sbjct: 481 GSGFGNFNSATDPARFNLVDPPLRNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSHLAWG 540

Query: 541 LAMVFLVENGEGEMQSVLPPPPDLPPC 568
           LAMVFLVENGEGEMQSVLPPPPDLPPC
Sbjct: 541 LAMVFLVENGEGEMQSVLPPPPDLPPC 567

BLAST of CmoCh01G007320.1 vs. NCBI nr
Match: XP_023520100.1 (laccase-13-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1066.2 bits (2756), Expect = 9.6e-308
Identity = 508/574 (88.50%), Postives = 537/574 (93.55%), Query Frame = 0

Query: 1   MEHLLKTKSLSSCFLLA----ILVLIPFAIAETHYHEFIVQRKPVKRLCKVHNIITVNGQ 60
           ME LLK KS  S FLL+    +L+L PFA+AETHYHEFIVQ KPVKRLCKVHNIITVNG+
Sbjct: 1   MEELLKIKSCCSLFLLSLLAFLLLLPPFAVAETHYHEFIVQPKPVKRLCKVHNIITVNGK 60

Query: 61  FPGPILEVRNGDSLVIKVVNAVRYNVSLHWHGIKQLRNPWADGPEFITQCSIKPGGSYTY 120
           FPGP L VRNGDSLVIKVVN  RYNVSLHWHGI+QLRNPWADGPEF+TQCSIKPGGSYTY
Sbjct: 61  FPGPTLAVRNGDSLVIKVVNVARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSYTY 120

Query: 121 RFTIEDQEGTLWWHAHSRWLRATVYGALIIYPKMGSYYPFLMPKREASVLLGEWFDRDPM 180
           RFTI+DQEGTLWWHAHSRWLRATVYGALIIYPK+ S YPFLMPKREASVLLGEWFDRDP+
Sbjct: 121 RFTIQDQEGTLWWHAHSRWLRATVYGALIIYPKLPSSYPFLMPKREASVLLGEWFDRDPI 180

Query: 181 SVLRQALLSGAAPNVSDAYTINGQPGDFYRCSKKETVRFGVNAGETILLRIVNSGLNQEL 240
           SVLRQAL +GAAPNVSDAYTINGQPGDFYRCS KETVRF V++GETILLRI+NS LNQEL
Sbjct: 181 SVLRQALFTGAAPNVSDAYTINGQPGDFYRCSGKETVRFAVDSGETILLRIINSALNQEL 240

Query: 241 FFSVANHPLTVVAVDASYTKPFKTNVIMIGPGQTTDVLLTANQPPAHYYMAATAYNTAMN 300
           FFSVANH LTVVAVDASYTKPF TNVIMIGPGQTTDVLLTANQ PAHYYMAATAYNTA+N
Sbjct: 241 FFSVANHQLTVVAVDASYTKPFTTNVIMIGPGQTTDVLLTANQQPAHYYMAATAYNTALN 300

Query: 301 ASFDNTTTTAILQYNNNYQQQ---PKPILAQLPFFNDTPTSTRFTAQLRSLNRANVPTQI 360
           A+FDNTTTTAIL+Y N  Q Q   P+PI+AQLP FNDTPT+TRFT+QLRS NR NVPTQI
Sbjct: 301 AAFDNTTTTAILEYKNCQQHQQHPPRPIMAQLPNFNDTPTATRFTSQLRSPNRVNVPTQI 360

Query: 361 DESLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNNSFVFPKSNSIMQAFYQGIPGVF 420
           D+SLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINN SFVFPKSNSIMQAFYQG+PGVF
Sbjct: 361 DDSLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNVSFVFPKSNSIMQAFYQGVPGVF 420

Query: 421 TTDFPPVPPLQFDYTGNVSRGLWQPGRGTKTFRLRYGSSVQIVLQDTSIVTTEDHPMHLH 480
           TTDFPPVPPLQFDYTGNVSRGLWQPGRGTK +RL+YGS+VQIVLQDTSIVTTEDHPMHLH
Sbjct: 421 TTDFPPVPPLQFDYTGNVSRGLWQPGRGTKGYRLKYGSNVQIVLQDTSIVTTEDHPMHLH 480

Query: 481 GYHFYVVGSGFGNFNSATDPARFNLVDPPMRNTIGTPPGGWVAIRFVADNPGAWLMHCHI 540
           GYHFYVVGSGFGNFN ATDPARFNL+DPP+RNTIGTPPGGWVAIRFVADNPGAWLMHCHI
Sbjct: 481 GYHFYVVGSGFGNFNPATDPARFNLIDPPVRNTIGTPPGGWVAIRFVADNPGAWLMHCHI 540

Query: 541 DSHLAWGLAMVFLVENGEGEMQSVLPPPPDLPPC 568
           DSHLAWGLAMVFLVENGEGE+QSVLPPPPDLPPC
Sbjct: 541 DSHLAWGLAMVFLVENGEGELQSVLPPPPDLPPC 574

BLAST of CmoCh01G007320.1 vs. TAIR 10
Match: AT2G30210.1 (laccase 3 )

HSP 1 Score: 877.1 bits (2265), Expect = 7.7e-255
Identity = 415/573 (72.43%), Postives = 469/573 (81.85%), Query Frame = 0

Query: 1   MEHLLKTKSLSSCFLLAILVLIPFAIAETHYHEFIVQRKPVKRLCKVHNIITVNGQFPGP 60
           ME   +   LS   LLA    +  A AE H H+F++   PVKRLC+ H  ITVNGQ+PGP
Sbjct: 1   MESFRRFSLLSFIALLAYFAFL--ASAEHHVHQFVITPTPVKRLCRTHQSITVNGQYPGP 60

Query: 61  ILEVRNGDSLVIKVVNAVRYNVSLHWHGIKQLRNPWADGPEFITQCSIKPGGSYTYRFTI 120
            L VRNGDSL I V+N  RYN+S+HWHGI+QLRNPWADGPE+ITQC I+PG +YTYRF I
Sbjct: 61  TLVVRNGDSLAITVINRARYNISIHWHGIRQLRNPWADGPEYITQCPIRPGQTYTYRFKI 120

Query: 121 EDQEGTLWWHAHSRWLRATVYGALIIYPKMGSYYPFLMPKREASVLLGEWFDRDPMSVLR 180
           EDQEGTLWWHAHSRWLRATVYGALIIYP++GS YPF MPKR+  +LLGEW+DR+PM VL+
Sbjct: 121 EDQEGTLWWHAHSRWLRATVYGALIIYPRLGSPYPFSMPKRDIPILLGEWWDRNPMDVLK 180

Query: 181 QALLSGAAPNVSDAYTINGQPGDFYRCSKKETVRFGVNAGETILLRIVNSGLNQELFFSV 240
           QA  +GAA NVSDAYTINGQPGD YRCS+  T+RF +  GET+ LR++N+G+NQELFFSV
Sbjct: 181 QAQFTGAAANVSDAYTINGQPGDLYRCSRAGTIRFPIFPGETVQLRVINAGMNQELFFSV 240

Query: 241 ANHPLTVVAVDASYTKPFKTNVIMIGPGQTTDVLLTANQPPAHYYMAATAYNTAMNASFD 300
           ANH  TVV  D++YTKPF TNVIMIGPGQTT+VLLTANQ P  YYMAA AYN+A NA FD
Sbjct: 241 ANHQFTVVETDSAYTKPFTTNVIMIGPGQTTNVLLTANQRPGRYYMAARAYNSA-NAPFD 300

Query: 301 NTTTTAILQYNN------NYQQQPKPILAQLPFFNDTPTSTRFTAQLRSLNRANVPTQID 360
           NTTTTAILQY N        + Q  P+   LP FNDT T+T FT +LR   RA VP Q+D
Sbjct: 301 NTTTTAILQYVNAPTRRGRGRGQIAPVFPVLPGFNDTATATAFTNRLRYWKRAPVPQQVD 360

Query: 361 ESLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNNSFVFPKSNSIMQAFYQGIPGVFT 420
           E+L FTVGLGLINC NPNSPRCQGPNGTRFAAS+NN SFV P+SNS+MQA+YQG PG+FT
Sbjct: 361 ENLFFTVGLGLINCANPNSPRCQGPNGTRFAASMNNMSFVLPRSNSVMQAYYQGTPGIFT 420

Query: 421 TDFPPVPPLQFDYTGNVSRGLWQPGRGTKTFRLRYGSSVQIVLQDTSIVTTEDHPMHLHG 480
           TDFPPVPP+QFDYTGNVSRGLWQP +GTK ++L+Y S+VQIVLQDTSIVT E+HPMHLHG
Sbjct: 421 TDFPPVPPVQFDYTGNVSRGLWQPIKGTKAYKLKYKSNVQIVLQDTSIVTPENHPMHLHG 480

Query: 481 YHFYVVGSGFGNFNSATDPARFNLVDPPMRNTIGTPPGGWVAIRFVADNPGAWLMHCHID 540
           Y FYVVGSGFGNFN  TDPARFNL DPP RNTIGTPPGGWVAIRFVADNPGAW MHCHID
Sbjct: 481 YQFYVVGSGFGNFNPRTDPARFNLFDPPERNTIGTPPGGWVAIRFVADNPGAWFMHCHID 540

Query: 541 SHLAWGLAMVFLVENGEGEMQSVLPPPPDLPPC 568
           SHL WGLAMVFLVENG G++QSV  PP DLP C
Sbjct: 541 SHLGWGLAMVFLVENGRGQLQSVQAPPLDLPRC 570

BLAST of CmoCh01G007320.1 vs. TAIR 10
Match: AT5G07130.1 (laccase 13 )

HSP 1 Score: 875.9 bits (2262), Expect = 1.7e-254
Identity = 413/569 (72.58%), Postives = 474/569 (83.30%), Query Frame = 0

Query: 8   KSLSSCFLLAILVLIPFAIAETHYHEFIVQRKPVKRLCKVHNIITVNGQFPGPILEVRNG 67
           + L   FLL  + +     AE H+HEF++Q  PVKRLC+VHN ITVNGQFPGP LEVRNG
Sbjct: 2   EQLRPFFLLLAIFVASLVNAEVHFHEFVIQETPVKRLCRVHNSITVNGQFPGPTLEVRNG 61

Query: 68  DSLVIKVVNAVRYNVSLHWHGIKQLRNPWADGPEFITQCSIKPGGSYTYRFTIEDQEGTL 127
           DSLVI  +N  RYN+SLHWHGI+Q+RNPWADGPE+ITQC I+PGGSYTYRFT+EDQEGTL
Sbjct: 62  DSLVITAINKARYNISLHWHGIRQMRNPWADGPEYITQCPIQPGGSYTYRFTMEDQEGTL 121

Query: 128 WWHAHSRWLRATVYGALIIYPKMGS-YYPF-LMPKREASVLLGEWFDRDPMSVLRQALLS 187
           WWHAHSRWLRATVYGALII P + S +YPF ++PKRE ++LLGEW+DR+PM VL  A  +
Sbjct: 122 WWHAHSRWLRATVYGALIIRPPLSSPHYPFPVIPKREITLLLGEWWDRNPMDVLNLAQFT 181

Query: 188 GAAPNVSDAYTINGQPGDFYRCSKKETVRFGVNAGETILLRIVNSGLNQELFFSVANHPL 247
           GAAPN+SDA+TINGQPGD YRCS +ET+RF V +GE +LLR++NS LNQELFF VANH L
Sbjct: 182 GAAPNISDAFTINGQPGDLYRCSSQETLRFLVGSGEIVLLRVINSALNQELFFGVANHKL 241

Query: 248 TVVAVDASYTKPFKTNVIMIGPGQTTDVLLTANQPPAHYYMAATAYNTAMNASFDNTTTT 307
           TVVA DASYTKPF TNVIM+GPGQTTDVLLTA+QPPAHYYMAA AYN+A NA+FDNTTTT
Sbjct: 242 TVVAADASYTKPFSTNVIMLGPGQTTDVLLTADQPPAHYYMAAHAYNSA-NAAFDNTTTT 301

Query: 308 AILQYNN------NYQQQPKPILAQLPFFNDTPTSTRFTAQLRSLNRANVPTQIDESLLF 367
           AIL+Y +        + Q + I AQLP FNDT T+  FTAQ++S ++  VP +IDE+L F
Sbjct: 302 AILKYKDASCVTLQAKSQARAIPAQLPGFNDTATAAAFTAQMKSPSKVKVPLEIDENLFF 361

Query: 368 TVGLGLINCTNPNSPRCQGPNGTRFAASINNNSFVFPKSNSIMQAFYQGIP-GVFTTDFP 427
           TVGLGL NC  PN+ RCQGPNGTRF ASINN SFVFPK NSIMQA+YQG P GVFTTDFP
Sbjct: 362 TVGLGLFNCPTPNTQRCQGPNGTRFTASINNVSFVFPKQNSIMQAYYQGTPTGVFTTDFP 421

Query: 428 PVPPLQFDYTGNVSRGLWQPGRGTKTFRLRYGSSVQIVLQDTSIVTTEDHPMHLHGYHFY 487
           P PP+ FDYTGNVSRGLWQP RGTK ++L++ S VQI+LQDTSIVTTE+HPMHLHGY FY
Sbjct: 422 PTPPVTFDYTGNVSRGLWQPTRGTKAYKLKFNSQVQIILQDTSIVTTENHPMHLHGYEFY 481

Query: 488 VVGSGFGNFNSATDPARFNLVDPPMRNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSHLA 547
           VVG+G GNFN  TD + FNL+DPP RNTIGTPPGGWVAIRFVA+NPGAWLMHCHIDSH+ 
Sbjct: 482 VVGTGVGNFNPNTDTSSFNLIDPPRRNTIGTPPGGWVAIRFVANNPGAWLMHCHIDSHIF 541

Query: 548 WGLAMVFLVENGEGEMQSVLPPPPDLPPC 568
           WGLAMVFLVENGEG +QSV  PP DLP C
Sbjct: 542 WGLAMVFLVENGEGHLQSVQSPPLDLPQC 569

BLAST of CmoCh01G007320.1 vs. TAIR 10
Match: AT2G40370.1 (laccase 5 )

HSP 1 Score: 773.5 bits (1996), Expect = 1.2e-223
Identity = 359/578 (62.11%), Postives = 439/578 (75.95%), Query Frame = 0

Query: 7   TKSLSSCFLLAILVLIPFAIAE---THYHEFIVQRKPVKRLCKVHNIITVNGQFPGPILE 66
           TKSL  CF+  +  L+  ++AE    H+HEFI+Q   VKRLC+ HN ITVNG FPGP+L 
Sbjct: 4   TKSL-LCFISFVAFLLFSSVAEANKAHHHEFIIQATKVKRLCETHNSITVNGMFPGPMLV 63

Query: 67  VRNGDSLVIKVVNAVRYNVSLHWHGIKQLRNPWADGPEFITQCSIKPGGSYTYRFTIEDQ 126
           V NGD+LV+KV+N  RYN+++HWHG++Q+R  WADGPEF+TQC I+PG SYTYRFTI+ Q
Sbjct: 64  VNNGDTLVVKVINRARYNITIHWHGVRQMRTGWADGPEFVTQCPIRPGSSYTYRFTIQGQ 123

Query: 127 EGTLWWHAHSRWLRATVYGALIIYPKMGSYYPFLMPKREASVLLGEWFDRDPMSVLRQAL 186
           EGTLWWHAHS WLRATVYG+L+++P  GS YPF  P R   +LLGEW+D +P+ VLR+++
Sbjct: 124 EGTLWWHAHSSWLRATVYGSLLVFPPAGSSYPFTKPHRNVPLLLGEWWDANPVDVLRESI 183

Query: 187 LSGAAPNVSDAYTINGQPGDFYRCSKKETVRFGVNAGETILLRIVNSGLNQELFFSVANH 246
            +G APN SDAYTINGQPGD Y+CS ++T    +N GETILLR++NS LNQ LFF+VANH
Sbjct: 184 RTGGAPNNSDAYTINGQPGDLYKCSSQDTTVVPINVGETILLRVINSALNQPLFFTVANH 243

Query: 247 PLTVVAVDASYTKPFKTNVIMIGPGQTTDVLLTANQPPAHYYMAATAYNTAMNASFDNTT 306
            LTVV  DASY KPF TNVI++GPGQTTDVL+T +QPP  YYMAA AY +A NA F NTT
Sbjct: 244 KLTVVGADASYLKPFTTNVIVLGPGQTTDVLITGDQPPNRYYMAARAYQSAQNAPFGNTT 303

Query: 307 TTAILQYNN------------NYQQQPKPILAQLPFFNDTPTSTRFTAQLRSLNRANVPT 366
           TTAILQY +                  KPI+  LP +NDT T TRF+   RSL RA VPT
Sbjct: 304 TTAILQYKSAPCCGVGGGSGTKKGNSFKPIMPILPAYNDTNTVTRFSQSFRSLRRAEVPT 363

Query: 367 QIDESLLFTVGLGLINC-TNPNSPRCQGPNGTRFAASINNNSFVFPKSNSIMQAFYQGIP 426
           +IDE+L  T+GLGL NC  N  S RCQGPNGTRF AS+NN SF  P + S++QA + GIP
Sbjct: 364 EIDENLFVTIGLGLNNCPKNFRSRRCQGPNGTRFTASMNNVSFALPSNYSLLQAHHHGIP 423

Query: 427 GVFTTDFPPVPPLQFDYTG-NVSRGLWQPGRGTKTFRLRYGSSVQIVLQDTSIVTTEDHP 486
           GVFTTDFP  PP++FDYTG N+SR L+QP RGTK ++L+YGS VQIVLQDT IVT E+HP
Sbjct: 424 GVFTTDFPAKPPVKFDYTGNNISRSLYQPDRGTKLYKLKYGSRVQIVLQDTGIVTPENHP 483

Query: 487 MHLHGYHFYVVGSGFGNFNSATDPARFNLVDPPMRNTIGTPPGGWVAIRFVADNPGAWLM 546
           +HLHGY FY++  GFGNFN   D A+FNL DPP+RNT+G P  GW  IRF+ADNPG W+M
Sbjct: 484 IHLHGYDFYIIAEGFGNFNPKKDTAKFNLEDPPLRNTVGVPVNGWAVIRFIADNPGVWIM 543

Query: 547 HCHIDSHLAWGLAMVFLVENGEGEMQSVLPPPPDLPPC 568
           HCH+D+H++WGLAM FLVENG G +Q++  PP DLP C
Sbjct: 544 HCHLDAHISWGLAMAFLVENGNGVLQTIEQPPHDLPVC 580

BLAST of CmoCh01G007320.1 vs. TAIR 10
Match: AT5G05390.1 (laccase 12 )

HSP 1 Score: 758.1 bits (1956), Expect = 5.2e-219
Identity = 349/545 (64.04%), Postives = 425/545 (77.98%), Query Frame = 0

Query: 26  IAETHYHEFIVQRKPVKRLCKVHNIITVNGQFPGPILEVRNGDSLVIKVVNAVRYNVSLH 85
           IA+  +H+F++Q  PVKRLCK  N ITVNG FPGP LEV NGD+L +KV N  RYN+++H
Sbjct: 23  IAKVQHHDFVIQETPVKRLCKTRNAITVNGMFPGPTLEVNNGDTLEVKVHNRARYNITIH 82

Query: 86  WHGIKQLRNPWADGPEFITQCSIKPGGSYTYRFTIEDQEGTLWWHAHSRWLRATVYGALI 145
           WHG++Q+R  WADGPEF+TQC I+PG SYTYRFTI+ QEGTLWWHAHS WLRATVYGALI
Sbjct: 83  WHGVRQIRTGWADGPEFVTQCPIRPGKSYTYRFTIQGQEGTLWWHAHSSWLRATVYGALI 142

Query: 146 IYPKMGSYYPFLMPKREASVLLGEWFDRDPMSVLRQALLSGAAPNVSDAYTINGQPGDFY 205
           I+P  GS +PF  P R+ +++LGEW++ +P+ V+ QA  +GAAPN+SDAYTINGQPGD Y
Sbjct: 143 IHPTPGSSFPFPKPDRQTALMLGEWWNANPVDVINQATRTGAAPNISDAYTINGQPGDLY 202

Query: 206 RCSKKETVRFGVNAGETILLRIVNSGLNQELFFSVANHPLTVVAVDASYTKPFKTNVIMI 265
            CS KETV   +N+GET LLR++N+ LNQ LFF+VANH LTVV  DASY KPF T V+M+
Sbjct: 203 NCSTKETVVVPINSGETSLLRVINAALNQPLFFTVANHKLTVVGADASYLKPFTTKVLML 262

Query: 266 GPGQTTDVLLTANQPPAHYYMAATAYNTAMNASFDNTTTTAILQYNNNYQQQPKPILAQL 325
           GPGQTTDVLLTA+QPP  YY+AA AY +A NA FDNTTTTAILQY        KPI+  L
Sbjct: 263 GPGQTTDVLLTADQPPKRYYIAARAYQSAQNAPFDNTTTTAILQYKKT-TTTSKPIMPVL 322

Query: 326 PFFNDTPTSTRFTAQLRSLNRANVPTQIDESLLFTVGLGLINCTN--PNSPRCQGPNGTR 385
           P FNDT T T F+ + +SL    VP  ID++L FT+GLGL NC    P S RCQG NGTR
Sbjct: 323 PAFNDTNTVTSFSRKFKSLRNVVVPKTIDDNLFFTIGLGLDNCPKKFPKS-RCQGLNGTR 382

Query: 386 FAASINNNSFVFPKSNSIMQAFYQGIPGVFTTDFPPVPPLQFDYTG-NVSRGLWQPGRGT 445
           F AS+NN SFV P + S++QA   GIPGVFTTDFP  PP++FDYTG N+SR L+QP +GT
Sbjct: 383 FTASMNNVSFVLPSNFSLLQAHSNGIPGVFTTDFPSKPPVKFDYTGNNISRALFQPVKGT 442

Query: 446 KTFRLRYGSSVQIVLQDTSIVTTEDHPMHLHGYHFYVVGSGFGNFNSATDPARFNLVDPP 505
           K ++L+YGS VQ+VLQDT+IVT+E+HP+HLHGY FY+VG GFGNFN   D ++FNLVDPP
Sbjct: 443 KLYKLKYGSRVQVVLQDTNIVTSENHPIHLHGYDFYIVGEGFGNFNPKKDTSKFNLVDPP 502

Query: 506 MRNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSHLAWGLAMVFLVENGEGEMQSVLPPPP 565
           +RNT+  P  GW  IRFVADNPG WLMHCH+D H+ WGLAM FLV+NG GE++++  PP 
Sbjct: 503 LRNTVAVPVNGWAVIRFVADNPGVWLMHCHLDVHIKWGLAMAFLVDNGVGELETLEAPPH 562

Query: 566 DLPPC 568
           DLP C
Sbjct: 563 DLPIC 565

BLAST of CmoCh01G007320.1 vs. TAIR 10
Match: AT5G09360.1 (laccase 14 )

HSP 1 Score: 616.7 bits (1589), Expect = 1.9e-176
Identity = 292/556 (52.52%), Postives = 386/556 (69.42%), Query Frame = 0

Query: 14  FLLAILVLIPFAIAETHYHEFIVQRKPVKRLCKVHNIITVNGQFPGPILEVRNGDSLVIK 73
           F L +L+    A AE H+H F ++ K   RLC  + I+TVNG+FPGP L+   GD L++ 
Sbjct: 20  FFLFVLLAFQIAEAEIHHHTFKIKSKAYTRLCNTNKILTVNGEFPGPTLKAYRGDKLIVN 79

Query: 74  VVNAVRYNVSLHWHGIKQLRNPWADGPEFITQCSIKPGGSYTYRFTIEDQEGTLWWHAHS 133
           V+N   YN++LHWHG +Q+RNPW+DGPE++TQC I+PG SY YR  ++ +EGT+WWHAHS
Sbjct: 80  VINNANYNITLHWHGARQIRNPWSDGPEYVTQCPIRPGESYVYRIDLKVEEGTIWWHAHS 139

Query: 134 RWLRATVYGALIIYPKMGSYYPFLMPKREASVLLGEWFDRDP-MSVLRQALLSGAAPNVS 193
           +W RATV+GA I+YPK GS YPF  P RE  ++LGEW+ ++  M +  +A  +G  P +S
Sbjct: 140 QWARATVHGAFIVYPKRGSSYPFPKPHREIPLILGEWWKKENIMHIPGKANKTGGEPAIS 199

Query: 194 DAYTINGQPGDFYRCSKKETVRFGVNAGETILLRIVNSGLNQELFFSVANHPLTVVAVDA 253
           D+YTINGQPG  Y CSK ET +  V  G   LLRI+N+ +++ELFF++ANH LTVVA D 
Sbjct: 200 DSYTINGQPGYLYPCSKPETFKITVVRGRRYLLRIINAVMDEELFFAIANHTLTVVAKDG 259

Query: 254 SYTKPFKTNVIMIGPGQTTDVLLTANQPPAHYYMAATAYNTAMNASFDNTTTTAILQYNN 313
            Y K FK++ +MI PGQ+ DVLL ANQ P HY++AA AY++A  A FD TTTTAILQY  
Sbjct: 260 FYLKHFKSDYLMITPGQSMDVLLHANQRPNHYFVAARAYSSAFGAGFDKTTTTAILQYKG 319

Query: 314 NYQQQPKPILAQLPFFNDTPTSTRFTAQLRSLNRANVPTQIDESLLFTVGLGLINCTNPN 373
           +   + KPIL  LP +N T  STRFT Q RS    NVP +I+  LL+ + + L+NC++  
Sbjct: 320 DTLNRIKPILPYLPPYNRTEASTRFTNQFRSQRPVNVPVKINTRLLYAISVNLMNCSDDR 379

Query: 374 SPRCQGPNGTRFAASINNNSFVFPKSNSIMQAFYQGIPGVFTTDFPPVPPLQFDYTGNVS 433
              C GP G RF++SINN SFV P S  I++A+Y+ I GVF  DFP  PP +F+YTG   
Sbjct: 380 P--CTGPFGKRFSSSINNISFVNP-SVDILRAYYRHIGGVFQEDFPRNPPTKFNYTG--- 439

Query: 434 RGLWQPGR-GTKTFRLRYGSSVQIVLQDTSIVTTEDHPMHLHGYHFYVVGSGFGNFNSAT 493
             L  P R GTK   L Y SSV+++LQ T++  +  HP+HLHGY+FYVVGSGFGNF+   
Sbjct: 440 ENLPFPTRFGTKVVVLDYNSSVELILQGTTVWASNIHPIHLHGYNFYVVGSGFGNFDRRK 499

Query: 494 DPARFNLVDPPMRNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSHLAWGLAMVFLVENGE 553
           DP R+NLVDPP   T+G P  GW A+RFVA+NPG WL+HCHI+ H  WG+  VF+V++G 
Sbjct: 500 DPLRYNLVDPPEETTVGVPRNGWTAVRFVANNPGVWLLHCHIERHATWGMNTVFIVKDGP 559

Query: 554 GEMQSVLPPPPDLPPC 568
            +   ++ PPPDLP C
Sbjct: 560 TKSSRMVKPPPDLPSC 569

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q56YT01.1e-25372.43Laccase-3 OS=Arabidopsis thaliana OX=3702 GN=LAC3 PE=2 SV=2[more]
Q9LYQ22.4e-25372.58Laccase-13 OS=Arabidopsis thaliana OX=3702 GN=LAC13 PE=2 SV=1[more]
Q9SIY81.7e-22262.11Laccase-5 OS=Arabidopsis thaliana OX=3702 GN=LAC5 PE=2 SV=1[more]
Q9FLB57.3e-21864.04Laccase-12 OS=Arabidopsis thaliana OX=3702 GN=LAC12 PE=2 SV=1[more]
Q941X26.4e-21461.90Laccase-3 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1G9570.0e+00100.00Laccase OS=Cucurbita moschata OX=3662 GN=LOC111452103 PE=3 SV=1[more]
A0A6J1K8J50.0e+0098.24Laccase OS=Cucurbita maxima OX=3661 GN=LOC111493234 PE=3 SV=1[more]
A0A6J1I6511.0e-30788.33Laccase OS=Cucurbita maxima OX=3661 GN=LOC111469556 PE=3 SV=1[more]
A0A6J1HPK11.1e-30687.80Laccase OS=Cucurbita moschata OX=3662 GN=LOC111464877 PE=3 SV=1[more]
A0A5D3BDS84.8e-28983.28Laccase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G00710 PE=3 S... [more]
Match NameE-valueIdentityDescription
XP_022948412.10.0e+00100.00laccase-3 [Cucurbita moschata][more]
KAG7037020.10.0e+0099.12Laccase-3, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022998647.10.0e+0098.24laccase-3 [Cucurbita maxima][more]
XP_023525776.10.0e+0098.24laccase-13-like [Cucurbita pepo subsp. pepo][more]
XP_023520100.19.6e-30888.50laccase-13-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT2G30210.17.7e-25572.43laccase 3 [more]
AT5G07130.11.7e-25472.58laccase 13 [more]
AT2G40370.11.2e-22362.11laccase 5 [more]
AT5G05390.15.2e-21964.04laccase 12 [more]
AT5G09360.11.9e-17652.52laccase 14 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011707Multicopper oxidase, N-termianlPFAMPF07732Cu-oxidase_3coord: 36..149
e-value: 7.5E-43
score: 145.3
IPR008972CupredoxinGENE3D2.60.40.420coord: 160..347
e-value: 1.2E-43
score: 151.5
IPR008972CupredoxinGENE3D2.60.40.420coord: 34..156
e-value: 4.0E-36
score: 126.1
IPR008972CupredoxinGENE3D2.60.40.420coord: 374..567
e-value: 4.0E-62
score: 211.6
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 29..175
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 160..344
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 350..567
IPR017761LaccaseTIGRFAMTIGR03389TIGR03389coord: 27..567
e-value: 1.1E-252
score: 837.6
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 165..313
e-value: 1.5E-41
score: 142.2
IPR011706Multicopper oxidase, C-terminalPFAMPF07731Cu-oxidase_2coord: 417..550
e-value: 2.6E-42
score: 143.8
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 8..567
NoneNo IPR availablePANTHERPTHR11709:SF68LACCASE-13coord: 8..567
IPR002355Multicopper oxidase, copper-binding sitePROSITEPS00080MULTICOPPER_OXIDASE2coord: 530..541
IPR033138Multicopper oxidases, conserved sitePROSITEPS00079MULTICOPPER_OXIDASE1coord: 525..545
IPR034289Laccase, third cupredoxin domainCDDcd13897CuRO_3_LCC_plantcoord: 412..550
e-value: 1.00544E-87
score: 265.662
IPR034288Laccase, first cupredoxin domainCDDcd13849CuRO_1_LCC_plantcoord: 32..148
e-value: 1.47194E-71
score: 222.905
IPR034285Laccase, second cupredoxin domainCDDcd13875CuRO_2_LCC_plantcoord: 165..310
e-value: 8.02586E-86
score: 260.994

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CmoCh01G007320CmoCh01G007320gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh01G007320.1:exon:4446CmoCh01G007320.1:exon:4446exon
CmoCh01G007320.1:exon:4445CmoCh01G007320.1:exon:4445exon
CmoCh01G007320.1:exon:4444CmoCh01G007320.1:exon:4444exon
CmoCh01G007320.1:exon:4443CmoCh01G007320.1:exon:4443exon
CmoCh01G007320.1:exon:4442CmoCh01G007320.1:exon:4442exon


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh01G007320.1:three_prime_utrCmoCh01G007320.1:three_prime_utrthree_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh01G007320.1:cdsCmoCh01G007320.1:cds_5CDS
CmoCh01G007320.1:cdsCmoCh01G007320.1:cds_4CDS
CmoCh01G007320.1:cdsCmoCh01G007320.1:cds_3CDS
CmoCh01G007320.1:cdsCmoCh01G007320.1:cds_2CDS
CmoCh01G007320.1:cdsCmoCh01G007320.1:cdsCDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CmoCh01G007320.1CmoCh01G007320.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046274 lignin catabolic process
cellular_component GO:0048046 apoplast
molecular_function GO:0005507 copper ion binding
molecular_function GO:0052716 hydroquinone:oxygen oxidoreductase activity
molecular_function GO:0016491 oxidoreductase activity