CmoCh01G005330.1 (mRNA) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh01G005330.1
Type	mRNA
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Carbonic anhydrase
Location	Cmo_Chr01: 2622368 .. 2624505 (+)
Sequence length	959
RNA-Seq Expression	CmoCh01G005330.1
Synteny	CmoCh01G005330.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGAACCCAAAGGTTTTAAAGATGGAGAAGCTCCCATTTCACGTCATTTTTGGCTCATTTTTATGTGGGTTTCTTCTGGTTTCGTGGCCTGTAATGTGTCAAGAAGTTGGTAGGGAACCCATTAAATCATCTTTTATTTCTCTATTTCTTCGTGTTTATGTTCGTTAAACGTAGAATAGATCATATCTGTACATAGTAAGATAATCTACTAATTTACTTGTTTGTTGCTATGAATTAAGGAGAATCTATGATTGAATAGATCATATCTGTAGATGTTACTTCTATAAATACATGAGTTTTTTAGATCAAAGTTAATATATTTTGTTTTGTTGTTTTGATTGGGAGCAGAAGATCAGAAGGAGTTTGATTATGACGCTGAAGGAAATAGAGGGCCTACTCATTGGGGAGATCTTAAACGAGAATGGCATGGCTGTAAGACTGGGTTGATGCAATCTCCTATTGATTTGTTGCACAAGAGAGTCCGAATTGTGTCCCATTTTACCAACCTCAAGATCGACTATAAACCCTCCAATGCAACTCTCAAGAACAGAGGTCATGATATCATGGTAACCAAAACAATATTTTCTTTCATTTTCGTGACATCCCATGTCAGTTGGAGAGGAAGACGAAACGTTCATTTATAAGAGTGTTGAAACCTCTCTCCTGGTAGATGGGTTTTAAAATCTTGAGGAGAAACCCATGAGAGAAAGCCCAAAGAGGACAATATAGGCTATCCATGCGCTTGAAGGTTACAAATGATATCAGAGATAGACACCGGTTGGTGTGCCCGCGAGGACGCTAGACCCCTAAAGAGGGTGGATTGTAAGATCCTACATCGGTTGGAAAGGGAACAAAACATTCGTATAAGTGTGTGGAAACCTCTTCCTATTAATTTTTGGCTTAGATATATTAAACCCTGGTCAATCAAACCCAATTTGTTCGATTTGAGTTGGGTTTGATTCGAATGAATAAATTTTTTTATAAATTGGGTTGGTTAATGACTCCGAACCACACCAACCCAAAAAATTTATGGTTAGATTGGATTGAAGATGTCTCAGTCCGACCCGACCCGACCCGACCCAACCCACGAACACTCCTAAGTTATATGATAATTTACAATTAGGTTTACGATAAAGAACGAACACTAATTTGATCTAATTTTATACCTCGAGAATGATACGTTAAAACTTTATGTAATTTAACCTAATTTGAACTACTTGTTGTGATGGTTGCAGCTGAAATGGAGTGGTGGAGCTGGATCTATAAATGTCAATGGAACTCGATACTTTCTAAGACAATGTCATTGGCACTCACCCTCTGAGCACACCATCAATGGAAGAAGGTTTGCCTTAGAAGCTCATTTGGTGCACCAAAGCCGAACTGGAAAAGTTGCTGTCATTGGACTTCTCTACAACATCGGACGACCAGACCATTTCTTGTCCAAAGTAACCCTCAAAACCTTAACCATTGTTTCAAAATTAAGGTTTGTTCTTCTACATGTTTGTCTTTGGAACTCATTTCCCTTTCATTTTTTTTTTAGTTGGAAGAGTATTTGGAAGATATATCGAATTCAAGGGAATACGATCAATTGAATGTGATCGATCCATCGTTGTTAAAGATGCAAAGCAGTTTATATTATCGATATATCGGCTCTCTTACCGTTCCTCCCTGTTCTCAAAATGTCCTTTGGACGATAGTGAGAGAGGTATTTTTTAATTGAACTTCTACGTCTTTGATCTCAATTTTACTCGGAATTGTATTTTCTATGGTTAAATGATCACTATCGTATATTTTTCAGATTAGGACGGTTGCACCCGAACAAGTGAAGTTGCTTCGTGTGGCTGTTCATGATGTAAGTGTGATTGTTTTTCATACGATAATGTTTTTTTTTTGTTCTTCTTTGGATTTTGTCTAATGCATCCTTTATATATGCAGGACTCGGACACTAATGCAAGACCTCTGCAACCTCTCAATGATCGAAAGATTCAACTTCGAATAAAAAACTAATCGTGGAGAATTGATTGCATCTACTATATGGAATAAATATTTATTGTATTTTTCATTCCAAACACCATGGCTATCATGCCATATCTTAATTTTTTTATATGTTAACACATGTTATGAAATTTATAATAAAC

mRNA sequence

AAGAACCCAAAGGTTTTAAAGATGGAGAAGCTCCCATTTCACGTCATTTTTGGCTCATTTTTATGTGGGTTTCTTCTGGTTTCGTGGCCTGTAATGTGTCAAGAAGTTGAAGATCAGAAGGAGTTTGATTATGACGCTGAAGGAAATAGAGGGCCTACTCATTGGGGAGATCTTAAACGAGAATGGCATGGCTGTAAGACTGGGTTGATGCAATCTCCTATTGATTTGTTGCACAAGAGAGTCCGAATTGTGTCCCATTTTACCAACCTCAAGATCGACTATAAACCCTCCAATGCAACTCTCAAGAACAGAGGTCATGATATCATGCTGAAATGGAGTGGTGGAGCTGGATCTATAAATGTCAATGGAACTCGATACTTTCTAAGACAATGTCATTGGCACTCACCCTCTGAGCACACCATCAATGGAAGAAGGTTTGCCTTAGAAGCTCATTTGGTGCACCAAAGCCGAACTGGAAAAGTTGCTGTCATTGGACTTCTCTACAACATCGGACGACCAGACCATTTCTTGTCCAAATTGGAAGAGTATTTGGAAGATATATCGAATTCAAGGGAATACGATCAATTGAATGTGATCGATCCATCGTTGTTAAAGATGCAAAGCAGTTTATATTATCGATATATCGGCTCTCTTACCGTTCCTCCCTGTTCTCAAAATGTCCTTTGGACGATAGTGAGAGAGATTAGGACGGTTGCACCCGAACAAGTGAAGTTGCTTCGTGTGGCTGTTCATGATGACTCGGACACTAATGCAAGACCTCTGCAACCTCTCAATGATCGAAAGATTCAACTTCGAATAAAAAACTAATCGTGGAGAATTGATTGCATCTACTATATGGAATAAATATTTATTGTATTTTTCATTCCAAACACCATGGCTATCATGCCATATCTTAATTTTTTTATATGTTAACACATGTTATGAAATTTATAATAAAC

Coding sequence (CDS)

ATGGAGAAGCTCCCATTTCACGTCATTTTTGGCTCATTTTTATGTGGGTTTCTTCTGGTTTCGTGGCCTGTAATGTGTCAAGAAGTTGAAGATCAGAAGGAGTTTGATTATGACGCTGAAGGAAATAGAGGGCCTACTCATTGGGGAGATCTTAAACGAGAATGGCATGGCTGTAAGACTGGGTTGATGCAATCTCCTATTGATTTGTTGCACAAGAGAGTCCGAATTGTGTCCCATTTTACCAACCTCAAGATCGACTATAAACCCTCCAATGCAACTCTCAAGAACAGAGGTCATGATATCATGCTGAAATGGAGTGGTGGAGCTGGATCTATAAATGTCAATGGAACTCGATACTTTCTAAGACAATGTCATTGGCACTCACCCTCTGAGCACACCATCAATGGAAGAAGGTTTGCCTTAGAAGCTCATTTGGTGCACCAAAGCCGAACTGGAAAAGTTGCTGTCATTGGACTTCTCTACAACATCGGACGACCAGACCATTTCTTGTCCAAATTGGAAGAGTATTTGGAAGATATATCGAATTCAAGGGAATACGATCAATTGAATGTGATCGATCCATCGTTGTTAAAGATGCAAAGCAGTTTATATTATCGATATATCGGCTCTCTTACCGTTCCTCCCTGTTCTCAAAATGTCCTTTGGACGATAGTGAGAGAGATTAGGACGGTTGCACCCGAACAAGTGAAGTTGCTTCGTGTGGCTGTTCATGATGACTCGGACACTAATGCAAGACCTCTGCAACCTCTCAATGATCGAAAGATTCAACTTCGAATAAAAAACTAA

Protein sequence

MEKLPFHVIFGSFLCGFLLVSWPVMCQEVEDQKEFDYDAEGNRGPTHWGDLKREWHGCKTGLMQSPIDLLHKRVRIVSHFTNLKIDYKPSNATLKNRGHDIMLKWSGGAGSINVNGTRYFLRQCHWHSPSEHTINGRRFALEAHLVHQSRTGKVAVIGLLYNIGRPDHFLSKLEEYLEDISNSREYDQLNVIDPSLLKMQSSLYYRYIGSLTVPPCSQNVLWTIVREIRTVAPEQVKLLRVAVHDDSDTNARPLQPLNDRKIQLRIKN

Homology

[Close]

BLAST of CmoCh01G005330.1 vs. ExPASy Swiss-Prot
Match: Q8L817 (Alpha carbonic anhydrase 7 OS=Arabidopsis thaliana OX=3702 GN=ACA7 PE=2 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 2.6e-80
Identity = 141/238 (59.24%), Postives = 180/238 (75.63%), Query Frame = 0


Query: 28  EVEDQKEFDYDAEGNRGPTHWGDLKREWHGCKTGLMQSPIDLLHKRVRIVSHFTNLKIDY 87
           EVED++EF+Y     +GP  WG+LK EW  C  G MQSPIDL+++RV IVSH   L  DY
Sbjct: 33  EVEDEREFNYKKNDEKGPERWGELKPEWEMCGKGEMQSPIDLMNERVNIVSHLGRLNRDY 92

Query: 88  KPSNATLKNRGHDIMLKWSGGAGSINVNGTRYFLRQCHWHSPSEHTINGRRFALEAHLVH 147
            PSNATLKNRGHDIMLK+  GAG+I +NG  Y L+Q HWHSPSEHTINGRRFALE H+VH
Sbjct: 93  NPSNATLKNRGHDIMLKFEDGAGTIKINGFEYELQQLHWHSPSEHTINGRRFALELHMVH 152

Query: 148 QSRTGKVAVIGLLYNIGRPDHFLSKLEEYLEDISNSREYDQ-LNVIDPSLLKMQSSLYYR 207
           + R  ++AV+ +LY IGR D F+  LE+ LE I+   E ++ + +IDP+ +K+ S  YYR
Sbjct: 153 EGRNRRMAVVTVLYKIGRADTFIRSLEKELEGIAEMEEAEKNVGMIDPTKIKIGSRKYYR 212

Query: 208 YIGSLTVPPCSQNVLWTIVREIRTVAPEQVKLLRVAVHDDSDTNARPLQPLNDRKIQL 265
           Y GSLT PPC+QNV W++VR++RTV  +QVKLLRVAVHDD+++NARP+QP N R + L
Sbjct: 213 YTGSLTTPPCTQNVTWSVVRKVRTVTRKQVKLLRVAVHDDANSNARPVQPTNKRIVHL 270

[Close]

BLAST of CmoCh01G005330.1 vs. ExPASy Swiss-Prot
Match: Q9FM99 (Alpha carbonic anhydrase 8 OS=Arabidopsis thaliana OX=3702 GN=ACA8 PE=3 SV=3)

HSP 1 Score: 281.6 bits (719), Expect = 9.5e-75
Identity = 137/240 (57.08%), Postives = 173/240 (72.08%), Query Frame = 0


Query: 28  EVEDQKEFDYDAEGNRGPTHWGDLKREWHGCKTGLMQSPIDLLHKRVRIVSHFTNLKIDY 87
           EVED+ EF Y+ +GN+GP  WG L  EW  C  G MQSPIDL  K V + + F  L+  Y
Sbjct: 133 EVEDETEFSYETKGNKGPAKWGTLDAEWKMCGIGKMQSPIDLRDKNVVVSNKFGLLRSQY 192

Query: 88  KPSNATLKNRGHDIMLKWSGGAGSINV--NGTRYFLRQCHWHSPSEHTINGRRFALEAHL 147
            PSN T+KNRGHDIMLK+ GG   I V   GTRY L+Q HWHSPSEHTING+RFALE HL
Sbjct: 193 LPSNTTIKNRGHDIMLKFKGGNKGIGVTIRGTRYQLQQLHWHSPSEHTINGKRFALEEHL 252

Query: 148 VHQSRTGKVAVIGLLYNIGRPDHFLSKLEEYLEDISNSREYDQ-LNVIDPSLLKMQSSLY 207
           VH+S+  + AV+  LYN+G  D FL  LE+ L+ I+++   ++ + +IDP  L  +S  Y
Sbjct: 253 VHESKDKRYAVVAFLYNLGASDPFLFSLEKQLKKITDTHASEEHVGIIDPKKLSFESKHY 312

Query: 208 YRYIGSLTVPPCSQNVLWTIVREIRTVAPEQVKLLRVAVHDDSDTNARPLQPLNDRKIQL 265
           YRY GSLT PPCS+NV+W++ +EIRTV+ +QVKLLRVAVHD SD+NARPLQ +N RK+ L
Sbjct: 313 YRYSGSLTAPPCSENVIWSVSKEIRTVSSKQVKLLRVAVHDASDSNARPLQAVNKRKVYL 372

[Close]

BLAST of CmoCh01G005330.1 vs. ExPASy Swiss-Prot
Match: F4HUC4 (Alpha carbonic anhydrase 5 OS=Arabidopsis thaliana OX=3702 GN=ACA5 PE=3 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 5.8e-72
Identity = 131/242 (54.13%), Postives = 175/242 (72.31%), Query Frame = 0


Query: 28  EVEDQKEFDYDAEGNRGPTHWGDLKREWHGCKTGLMQSPIDLLHKRVRIVSHFTNLKIDY 87
           EVED+ +F+Y+ +G +GP +WG LK EW  C  G MQSPIDL  KRV I  +   L+  Y
Sbjct: 28  EVEDETQFNYEKKGEKGPENWGRLKPEWAMCGKGNMQSPIDLTDKRVLIDHNLGYLRSQY 87

Query: 88  KPSNATLKNRGHDIMLKWSGG-AG-SINVNGTRYFLRQCHWHSPSEHTINGRRFALEAHL 147
            PSNAT+KNRGHDIM+K+ GG AG  I +NGT Y L+Q HWHSPSEHT+NG+RF LE H+
Sbjct: 88  LPSNATIKNRGHDIMMKFEGGNAGLGITINGTEYKLQQIHWHSPSEHTLNGKRFVLEEHM 147

Query: 148 VHQSRTGKVAVIGLLYNIGRPDHFLSKLEEYLEDISNSREYDQ-LNVIDPSLLKMQSSLY 207
           VHQS+ G+ AV+   Y +G+PD+FL  LE YL+ I+++ E  + + ++ P     +S  Y
Sbjct: 148 VHQSKDGRNAVVAFFYKLGKPDYFLLTLERYLKRITDTHESQEFVEMVHPRTFGFESKHY 207

Query: 208 YRYIGSLTVPPCSQNVLWTIVREIRTVAPEQVKLLRVAVHDDSDTNARPLQPLNDRKIQL 267
           YR+IGSLT PPCS+NV+WTI +E+RTV  +Q+ +LRV VHD S++NARPLQ  N+R + L
Sbjct: 208 YRFIGSLTTPPCSENVIWTISKEMRTVTLKQLIMLRVTVHDQSNSNARPLQRKNERPVAL 267

[Close]

BLAST of CmoCh01G005330.1 vs. ExPASy Swiss-Prot
Match: Q84UV8 (Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3 OS=Nicotiana langsdorffii x Nicotiana sanderae OX=164110 GN=NEC3 PE=1 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 1.9e-67
Identity = 129/257 (50.19%), Postives = 177/257 (68.87%), Query Frame = 0


Query: 8   VIFGSFLCGFLLVSWPVMCQEVEDQKEFDYDAEGNRGPTHWGDLKREWHGCKTGLMQSPI 67
           ++F SFL  FL   +     EV+D+ EF YD +   GP +WG+++ +W  C +G +QSPI
Sbjct: 9   MLFISFL--FLSSVFLARSGEVDDESEFSYDEKSENGPANWGNIRPDWKEC-SGKLQSPI 68

Query: 68  DLLHKRVRIVSHFTNLKIDYKPSNATLKNRGHDIMLKWSGGAGSINVNGTRYFLRQCHWH 127
           D+   R  +VS+   L+ DYKPSNATL NRGHDIML+   G G + +N T+Y L+Q HWH
Sbjct: 69  DIFDLRAEVVSNLRILQKDYKPSNATLLNRGHDIMLRLDDG-GYLKINETQYQLKQLHWH 128

Query: 128 SPSEHTINGRRFALEAHLVHQSRTGKVAVIGLLYNIGR-PDHFLSKLEEYLEDISNSREY 187
           +PSEHTING RF LEAHLVH+S  GK  VIG++Y IG  PD FLS +E  L+  +N +  
Sbjct: 129 TPSEHTINGERFNLEAHLVHESNNGKFVVIGIVYEIGLWPDPFLSMIENDLKVPANKKGI 188

Query: 188 DQ-LNVIDPSLLKMQSSLYYRYIGSLTVPPCSQNVLWTIVREIRTVAPEQVKLLRVAVHD 247
           ++ + +IDP+ +K+    Y+RYIGSLT PPC++ V+W I R+++TV   Q+KLL+ AVHD
Sbjct: 189 ERGIGIIDPNQIKLDGKKYFRYIGSLTTPPCTEGVVWIIDRKVKTVTRRQIKLLQEAVHD 248

Query: 248 DSDTNARPLQPLNDRKI 263
             +TNARP QP N+R I
Sbjct: 249 GFETNARPTQPENERYI 261

[Close]

BLAST of CmoCh01G005330.1 vs. ExPASy Swiss-Prot
Match: F4IHR4 (Alpha carbonic anhydrase 2 OS=Arabidopsis thaliana OX=3702 GN=ACA2 PE=2 SV=2)

HSP 1 Score: 248.4 bits (633), Expect = 8.9e-65
Identity = 121/225 (53.78%), Postives = 158/225 (70.22%), Query Frame = 0


Query: 8   VIFGSFLCGFLLVSWPVMCQEVEDQKEFDYDAEGNRGPTHWGDLKREWHGCKTGLMQSPI 67
           ++  SF+     +S     +EVED+ EF Y+     GP  WG L+ EW  C  G MQSPI
Sbjct: 12  LVLTSFVTTVSCLSAATDYREVEDEHEFSYEWNQENGPAKWGKLRPEWKMCGKGEMQSPI 71

Query: 68  DLLHKRVRIVSHFTNLKIDYKPSNATLKNRGHDIMLKW-SGGAGSINVNGTRYFLRQCHW 127
           DL++KRVR+V+H   L   YKP NATLKNRGHD+MLK+   G+GSI VNGT Y L Q HW
Sbjct: 72  DLMNKRVRLVTHLKKLTRHYKPCNATLKNRGHDMMLKFGEEGSGSITVNGTEYKLLQLHW 131

Query: 128 HSPSEHTINGRRFALEAHLVHQSRTGKVAVIGLLYNIGRPDHFLSKLEEYLEDISNSREY 187
           HSPSEHT+NGRRFALE H+VH++  G +AV+ +LY IGRPD FL  LE  L  I++  E 
Sbjct: 132 HSPSEHTMNGRRFALELHMVHENINGSLAVVTVLYKIGRPDSFLGLLENKLSAITDQNEA 191

Query: 188 DQ-LNVIDPSLLKMQSSLYYRYIGSLTVPPCSQNVLWTIVREIRT 231
           ++ ++VIDP  +K+ S  +YRYIGSLT PPC+QNV+WT+V+++ T
Sbjct: 192 EKYVDVIDPRDIKIGSRKFYRYIGSLTTPPCTQNVIWTVVKKVNT 236

[Close]

BLAST of CmoCh01G005330.1 vs. ExPASy TrEMBL
Match: A0A6J1G941 (Carbonic anhydrase OS=Cucurbita moschata OX=3662 GN=LOC111452083 PE=3 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 1.8e-156
Identity = 268/268 (100.00%), Postives = 268/268 (100.00%), Query Frame = 0


Query: 1   MEKLPFHVIFGSFLCGFLLVSWPVMCQEVEDQKEFDYDAEGNRGPTHWGDLKREWHGCKT 60
           MEKLPFHVIFGSFLCGFLLVSWPVMCQEVEDQKEFDYDAEGNRGPTHWGDLKREWHGCKT
Sbjct: 1   MEKLPFHVIFGSFLCGFLLVSWPVMCQEVEDQKEFDYDAEGNRGPTHWGDLKREWHGCKT 60

Query: 61  GLMQSPIDLLHKRVRIVSHFTNLKIDYKPSNATLKNRGHDIMLKWSGGAGSINVNGTRYF 120
           GLMQSPIDLLHKRVRIVSHFTNLKIDYKPSNATLKNRGHDIMLKWSGGAGSINVNGTRYF
Sbjct: 61  GLMQSPIDLLHKRVRIVSHFTNLKIDYKPSNATLKNRGHDIMLKWSGGAGSINVNGTRYF 120

Query: 121 LRQCHWHSPSEHTINGRRFALEAHLVHQSRTGKVAVIGLLYNIGRPDHFLSKLEEYLEDI 180
           LRQCHWHSPSEHTINGRRFALEAHLVHQSRTGKVAVIGLLYNIGRPDHFLSKLEEYLEDI
Sbjct: 121 LRQCHWHSPSEHTINGRRFALEAHLVHQSRTGKVAVIGLLYNIGRPDHFLSKLEEYLEDI 180

Query: 181 SNSREYDQLNVIDPSLLKMQSSLYYRYIGSLTVPPCSQNVLWTIVREIRTVAPEQVKLLR 240
           SNSREYDQLNVIDPSLLKMQSSLYYRYIGSLTVPPCSQNVLWTIVREIRTVAPEQVKLLR
Sbjct: 181 SNSREYDQLNVIDPSLLKMQSSLYYRYIGSLTVPPCSQNVLWTIVREIRTVAPEQVKLLR 240

Query: 241 VAVHDDSDTNARPLQPLNDRKIQLRIKN 269
           VAVHDDSDTNARPLQPLNDRKIQLRIKN
Sbjct: 241 VAVHDDSDTNARPLQPLNDRKIQLRIKN 268

[Close]

BLAST of CmoCh01G005330.1 vs. ExPASy TrEMBL
Match: A0A6J1KFK2 (Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111493412 PE=3 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 4.8e-154
Identity = 263/268 (98.13%), Postives = 267/268 (99.63%), Query Frame = 0


Query: 1   MEKLPFHVIFGSFLCGFLLVSWPVMCQEVEDQKEFDYDAEGNRGPTHWGDLKREWHGCKT 60
           MEKLPFHVIFG+FLC FLLVSWPVMCQEVEDQKEFDYDAEG+RGPTHWGDLKREWHGCKT
Sbjct: 1   MEKLPFHVIFGAFLCAFLLVSWPVMCQEVEDQKEFDYDAEGDRGPTHWGDLKREWHGCKT 60

Query: 61  GLMQSPIDLLHKRVRIVSHFTNLKIDYKPSNATLKNRGHDIMLKWSGGAGSINVNGTRYF 120
           GLMQSPIDLLHKRVRIVSHFTNLKIDYKPSNATLKNRGHDIMLKWSGGAGSINVNGTRYF
Sbjct: 61  GLMQSPIDLLHKRVRIVSHFTNLKIDYKPSNATLKNRGHDIMLKWSGGAGSINVNGTRYF 120

Query: 121 LRQCHWHSPSEHTINGRRFALEAHLVHQSRTGKVAVIGLLYNIGRPDHFLSKLEEYLEDI 180
           LRQCHWHSPSEHTINGRRFALEAHLVHQS+TGKVAVIGLLYNIGRPDHFLSKLEEYLEDI
Sbjct: 121 LRQCHWHSPSEHTINGRRFALEAHLVHQSQTGKVAVIGLLYNIGRPDHFLSKLEEYLEDI 180

Query: 181 SNSREYDQLNVIDPSLLKMQSSLYYRYIGSLTVPPCSQNVLWTIVREIRTVAPEQVKLLR 240
           SNSREYD+LNVIDPSLLKMQSSLYYRYIGSLTVPPCSQNVLWTIVREIRTVAPEQVKLLR
Sbjct: 181 SNSREYDELNVIDPSLLKMQSSLYYRYIGSLTVPPCSQNVLWTIVREIRTVAPEQVKLLR 240

Query: 241 VAVHDDSDTNARPLQPLNDRKIQLRIKN 269
           VAVHDDSDTNARPLQPLNDRKIQLRIKN
Sbjct: 241 VAVHDDSDTNARPLQPLNDRKIQLRIKN 268

[Close]

BLAST of CmoCh01G005330.1 vs. ExPASy TrEMBL
Match: A0A6J1HYT6 (Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111469328 PE=3 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 5.5e-126
Identity = 217/268 (80.97%), Postives = 240/268 (89.55%), Query Frame = 0


Query: 1   MEKLPFHVIFGSFLCGFLLVSWPVMCQEVEDQKEFDYDAEGNRGPTHWGDLKREWHGCKT 60
           M+KL FH +F +FLC FLLVSW  M QEVEDQKEFDY+ EG RGP+HWGDLKREWH CK 
Sbjct: 1   MKKLSFHALFCTFLCAFLLVSWRAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60

Query: 61  GLMQSPIDLLHKRVRIVSHFTNLKIDYKPSNATLKNRGHDIMLKWSGGAGSINVNGTRYF 120
           GLMQSPIDLLH+RVRIV HFTNLK +YKP NATLKNRGHDIMLKW  GAG + VNGTRYF
Sbjct: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPCNATLKNRGHDIMLKWGEGAGYMEVNGTRYF 120

Query: 121 LRQCHWHSPSEHTINGRRFALEAHLVHQSRTGKVAVIGLLYNIGRPDHFLSKLEEYLEDI 180
           L+Q HWHSPSEHTINGRRFALEAHLVHQS TG VAVIG+LY IGRPD+FLSK+EEYLE++
Sbjct: 121 LKQFHWHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKMEEYLEEV 180

Query: 181 SNSREYDQLNVIDPSLLKMQSSLYYRYIGSLTVPPCSQNVLWTIVREIRTVAPEQVKLLR 240
           S++RE   +++ DPS L+M SSLYYRYIGSLTVPPCSQNVLWTIVR+IRTV+PEQVKLLR
Sbjct: 181 SDTREDKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKIRTVSPEQVKLLR 240

Query: 241 VAVHDDSDTNARPLQPLNDRKIQLRIKN 269
           VAVHDDSDTNARPLQPLNDRKIQLRIK+
Sbjct: 241 VAVHDDSDTNARPLQPLNDRKIQLRIKS 268

[Close]

BLAST of CmoCh01G005330.1 vs. ExPASy TrEMBL
Match: A0A6J1I3K8 (Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111469329 PE=3 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 8.0e-125
Identity = 214/268 (79.85%), Postives = 240/268 (89.55%), Query Frame = 0


Query: 1   MEKLPFHVIFGSFLCGFLLVSWPVMCQEVEDQKEFDYDAEGNRGPTHWGDLKREWHGCKT 60
           M+KL FH +F +FLC FLLVSW  M QEVEDQKEFDY+ EG RGP+HWGDLKREWH CK 
Sbjct: 1   MKKLSFHALFCTFLCAFLLVSWRAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60

Query: 61  GLMQSPIDLLHKRVRIVSHFTNLKIDYKPSNATLKNRGHDIMLKWSGGAGSINVNGTRYF 120
           GLMQSPIDLLH+RVRIV HFTNLK +YKP NATLKNRGHDIMLKW  GAG + VNGTRYF
Sbjct: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPCNATLKNRGHDIMLKWGEGAGYMEVNGTRYF 120

Query: 121 LRQCHWHSPSEHTINGRRFALEAHLVHQSRTGKVAVIGLLYNIGRPDHFLSKLEEYLEDI 180
           L+Q HWHSPSEHTINGRRFALEAHLVHQS TG VAVIG+LY IGRPD+FLSK+EE+LE++
Sbjct: 121 LKQFHWHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKMEEHLEEV 180

Query: 181 SNSREYDQLNVIDPSLLKMQSSLYYRYIGSLTVPPCSQNVLWTIVREIRTVAPEQVKLLR 240
           S+++E   +++ DPS L+M SSLYYRYIGSLTVPPCSQNVLWTIVR+IRTV+PEQVKLLR
Sbjct: 181 SDTQEDKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKIRTVSPEQVKLLR 240

Query: 241 VAVHDDSDTNARPLQPLNDRKIQLRIKN 269
           VAVHDDSDTNARPLQPLNDRKIQLR+K+
Sbjct: 241 VAVHDDSDTNARPLQPLNDRKIQLRVKS 268

[Close]

BLAST of CmoCh01G005330.1 vs. ExPASy TrEMBL
Match: A0A6J1HKB2 (Carbonic anhydrase OS=Cucurbita moschata OX=3662 GN=LOC111464883 PE=3 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 2.6e-123
Identity = 209/268 (77.99%), Postives = 240/268 (89.55%), Query Frame = 0


Query: 1   MEKLPFHVIFGSFLCGFLLVSWPVMCQEVEDQKEFDYDAEGNRGPTHWGDLKREWHGCKT 60
           MEKL FH +F +FLC FL+VSWP M QEVEDQKEFDY+ EG RGP+HWGDLKREWH CK 
Sbjct: 1   MEKLSFHALFCTFLCAFLMVSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60

Query: 61  GLMQSPIDLLHKRVRIVSHFTNLKIDYKPSNATLKNRGHDIMLKWSGGAGSINVNGTRYF 120
           GLMQSPIDLLH+RVRIV HFTNLK +YKPSNATLKNRGHDIMLKW  GAG + VNGTRYF
Sbjct: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKWGEGAGYMEVNGTRYF 120

Query: 121 LRQCHWHSPSEHTINGRRFALEAHLVHQSRTGKVAVIGLLYNIGRPDHFLSKLEEYLEDI 180
           L+Q HWHSPSEHTINGRRFALEAHLVHQS TG VAVIG+LY IGRPD+FLSK++EYLE++
Sbjct: 121 LKQFHWHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKMKEYLEEV 180

Query: 181 SNSREYDQLNVIDPSLLKMQSSLYYRYIGSLTVPPCSQNVLWTIVREIRTVAPEQVKLLR 240
            +++E   +++  PS L+M SSLYY+YIGSLTVPPCSQNVLWTIVR+++TV+PEQVKLLR
Sbjct: 181 CDTKEDKAIDLGHPSQLEMLSSLYYQYIGSLTVPPCSQNVLWTIVRKVKTVSPEQVKLLR 240

Query: 241 VAVHDDSDTNARPLQPLNDRKIQLRIKN 269
           VAVHDDSDTNARPLQPL+DR+IQLR+K+
Sbjct: 241 VAVHDDSDTNARPLQPLHDRRIQLRVKS 268

[Close]

BLAST of CmoCh01G005330.1 vs. NCBI nr
Match: XP_022948392.1 (alpha carbonic anhydrase 7-like [Cucurbita moschata] >KAG6607147.1 Alpha carbonic anhydrase 7, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036836.1 Alpha carbonic anhydrase 7 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 561.6 bits (1446), Expect = 3.6e-156
Identity = 268/268 (100.00%), Postives = 268/268 (100.00%), Query Frame = 0


Query: 1   MEKLPFHVIFGSFLCGFLLVSWPVMCQEVEDQKEFDYDAEGNRGPTHWGDLKREWHGCKT 60
           MEKLPFHVIFGSFLCGFLLVSWPVMCQEVEDQKEFDYDAEGNRGPTHWGDLKREWHGCKT
Sbjct: 1   MEKLPFHVIFGSFLCGFLLVSWPVMCQEVEDQKEFDYDAEGNRGPTHWGDLKREWHGCKT 60

Query: 61  GLMQSPIDLLHKRVRIVSHFTNLKIDYKPSNATLKNRGHDIMLKWSGGAGSINVNGTRYF 120
           GLMQSPIDLLHKRVRIVSHFTNLKIDYKPSNATLKNRGHDIMLKWSGGAGSINVNGTRYF
Sbjct: 61  GLMQSPIDLLHKRVRIVSHFTNLKIDYKPSNATLKNRGHDIMLKWSGGAGSINVNGTRYF 120

Query: 121 LRQCHWHSPSEHTINGRRFALEAHLVHQSRTGKVAVIGLLYNIGRPDHFLSKLEEYLEDI 180
           LRQCHWHSPSEHTINGRRFALEAHLVHQSRTGKVAVIGLLYNIGRPDHFLSKLEEYLEDI
Sbjct: 121 LRQCHWHSPSEHTINGRRFALEAHLVHQSRTGKVAVIGLLYNIGRPDHFLSKLEEYLEDI 180

Query: 181 SNSREYDQLNVIDPSLLKMQSSLYYRYIGSLTVPPCSQNVLWTIVREIRTVAPEQVKLLR 240
           SNSREYDQLNVIDPSLLKMQSSLYYRYIGSLTVPPCSQNVLWTIVREIRTVAPEQVKLLR
Sbjct: 181 SNSREYDQLNVIDPSLLKMQSSLYYRYIGSLTVPPCSQNVLWTIVREIRTVAPEQVKLLR 240

Query: 241 VAVHDDSDTNARPLQPLNDRKIQLRIKN 269
           VAVHDDSDTNARPLQPLNDRKIQLRIKN
Sbjct: 241 VAVHDDSDTNARPLQPLNDRKIQLRIKN 268

[Close]

BLAST of CmoCh01G005330.1 vs. NCBI nr
Match: XP_022998889.1 (alpha carbonic anhydrase 7-like [Cucurbita maxima])

HSP 1 Score: 553.5 bits (1425), Expect = 9.9e-154
Identity = 263/268 (98.13%), Postives = 267/268 (99.63%), Query Frame = 0


Query: 1   MEKLPFHVIFGSFLCGFLLVSWPVMCQEVEDQKEFDYDAEGNRGPTHWGDLKREWHGCKT 60
           MEKLPFHVIFG+FLC FLLVSWPVMCQEVEDQKEFDYDAEG+RGPTHWGDLKREWHGCKT
Sbjct: 1   MEKLPFHVIFGAFLCAFLLVSWPVMCQEVEDQKEFDYDAEGDRGPTHWGDLKREWHGCKT 60

Query: 61  GLMQSPIDLLHKRVRIVSHFTNLKIDYKPSNATLKNRGHDIMLKWSGGAGSINVNGTRYF 120
           GLMQSPIDLLHKRVRIVSHFTNLKIDYKPSNATLKNRGHDIMLKWSGGAGSINVNGTRYF
Sbjct: 61  GLMQSPIDLLHKRVRIVSHFTNLKIDYKPSNATLKNRGHDIMLKWSGGAGSINVNGTRYF 120

Query: 121 LRQCHWHSPSEHTINGRRFALEAHLVHQSRTGKVAVIGLLYNIGRPDHFLSKLEEYLEDI 180
           LRQCHWHSPSEHTINGRRFALEAHLVHQS+TGKVAVIGLLYNIGRPDHFLSKLEEYLEDI
Sbjct: 121 LRQCHWHSPSEHTINGRRFALEAHLVHQSQTGKVAVIGLLYNIGRPDHFLSKLEEYLEDI 180

Query: 181 SNSREYDQLNVIDPSLLKMQSSLYYRYIGSLTVPPCSQNVLWTIVREIRTVAPEQVKLLR 240
           SNSREYD+LNVIDPSLLKMQSSLYYRYIGSLTVPPCSQNVLWTIVREIRTVAPEQVKLLR
Sbjct: 181 SNSREYDELNVIDPSLLKMQSSLYYRYIGSLTVPPCSQNVLWTIVREIRTVAPEQVKLLR 240

Query: 241 VAVHDDSDTNARPLQPLNDRKIQLRIKN 269
           VAVHDDSDTNARPLQPLNDRKIQLRIKN
Sbjct: 241 VAVHDDSDTNARPLQPLNDRKIQLRIKN 268

[Close]

BLAST of CmoCh01G005330.1 vs. NCBI nr
Match: XP_023523228.1 (alpha carbonic anhydrase 7-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 549.7 bits (1415), Expect = 1.4e-152
Identity = 262/268 (97.76%), Postives = 266/268 (99.25%), Query Frame = 0


Query: 1   MEKLPFHVIFGSFLCGFLLVSWPVMCQEVEDQKEFDYDAEGNRGPTHWGDLKREWHGCKT 60
           M KLPFHVIFGSFLC FLLVSWPVMCQEVEDQKEFDYDAEG+RGPTHWG+LKREWHGCKT
Sbjct: 1   MVKLPFHVIFGSFLCAFLLVSWPVMCQEVEDQKEFDYDAEGDRGPTHWGELKREWHGCKT 60

Query: 61  GLMQSPIDLLHKRVRIVSHFTNLKIDYKPSNATLKNRGHDIMLKWSGGAGSINVNGTRYF 120
           GLMQSPIDLLHKRVRIVSHFTNLKIDYKPSNATLKNRGHDIMLKWSGGAGSINVNGTRYF
Sbjct: 61  GLMQSPIDLLHKRVRIVSHFTNLKIDYKPSNATLKNRGHDIMLKWSGGAGSINVNGTRYF 120

Query: 121 LRQCHWHSPSEHTINGRRFALEAHLVHQSRTGKVAVIGLLYNIGRPDHFLSKLEEYLEDI 180
           LRQCHWHSPSEHTINGRRFALEAHLVHQSRTGKVAVIGLLYNIGRPDHFLSKLEEYLEDI
Sbjct: 121 LRQCHWHSPSEHTINGRRFALEAHLVHQSRTGKVAVIGLLYNIGRPDHFLSKLEEYLEDI 180

Query: 181 SNSREYDQLNVIDPSLLKMQSSLYYRYIGSLTVPPCSQNVLWTIVREIRTVAPEQVKLLR 240
           S+SREYD+LNVIDPSLLKMQSSLYYRYIGSLTVPPCSQNVLWTIVREIRTVAPEQVKLLR
Sbjct: 181 SDSREYDKLNVIDPSLLKMQSSLYYRYIGSLTVPPCSQNVLWTIVREIRTVAPEQVKLLR 240

Query: 241 VAVHDDSDTNARPLQPLNDRKIQLRIKN 269
           VAVHDDSDTNARPLQPLNDRKIQLRIKN
Sbjct: 241 VAVHDDSDTNARPLQPLNDRKIQLRIKN 268

[Close]

BLAST of CmoCh01G005330.1 vs. NCBI nr
Match: XP_023519286.1 (alpha carbonic anhydrase 7-like [Cucurbita pepo subsp. pepo] >XP_023519287.1 alpha carbonic anhydrase 7-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 461.8 bits (1187), Expect = 3.9e-126
Identity = 214/268 (79.85%), Postives = 243/268 (90.67%), Query Frame = 0


Query: 1   MEKLPFHVIFGSFLCGFLLVSWPVMCQEVEDQKEFDYDAEGNRGPTHWGDLKREWHGCKT 60
           MEKL FH +F +FLC FLLVSW  M QEVEDQKEFDY+ EG RGP+HWGDLKREWH CK 
Sbjct: 1   MEKLSFHALFCTFLCAFLLVSWRAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60

Query: 61  GLMQSPIDLLHKRVRIVSHFTNLKIDYKPSNATLKNRGHDIMLKWSGGAGSINVNGTRYF 120
           GLMQSPIDLLH+RVRIV HFTNLK +YKPSNATLKNRGHDIMLKW  GAG + VNGTRYF
Sbjct: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKWGEGAGYMEVNGTRYF 120

Query: 121 LRQCHWHSPSEHTINGRRFALEAHLVHQSRTGKVAVIGLLYNIGRPDHFLSKLEEYLEDI 180
           L+Q HWHSPSEHTINGRRFALEAHLVHQS TG VAVIG+LY IGRPD+FLSK++EYLE++
Sbjct: 121 LKQFHWHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKMKEYLEEV 180

Query: 181 SNSREYDQLNVIDPSLLKMQSSLYYRYIGSLTVPPCSQNVLWTIVREIRTVAPEQVKLLR 240
           S+++E + +++ DPS L+M+SSLYYRYIGSLTVPPCSQNVLWT+VR++RTV+PEQVKLLR
Sbjct: 181 SDTKEDEPIDLADPSQLEMRSSLYYRYIGSLTVPPCSQNVLWTLVRKVRTVSPEQVKLLR 240

Query: 241 VAVHDDSDTNARPLQPLNDRKIQLRIKN 269
           VAVHDDSDTNARPLQPLNDRKIQLR+K+
Sbjct: 241 VAVHDDSDTNARPLQPLNDRKIQLRVKS 268

[Close]

BLAST of CmoCh01G005330.1 vs. NCBI nr
Match: KAG7020784.1 (Alpha carbonic anhydrase 7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 461.5 bits (1186), Expect = 5.1e-126
Identity = 215/268 (80.22%), Postives = 241/268 (89.93%), Query Frame = 0


Query: 1   MEKLPFHVIFGSFLCGFLLVSWPVMCQEVEDQKEFDYDAEGNRGPTHWGDLKREWHGCKT 60
           MEKL FH +F +FLC FLLVSWP M QEVEDQKEFDY+ EG RGP+HWGDLKREWH CK 
Sbjct: 1   MEKLSFHALFCTFLCAFLLVSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60

Query: 61  GLMQSPIDLLHKRVRIVSHFTNLKIDYKPSNATLKNRGHDIMLKWSGGAGSINVNGTRYF 120
           GLMQSPIDLLH+RVRIV HFTNLK +YKPSNATLKNRGHDIMLKW  GAG + VNGTRYF
Sbjct: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKWGEGAGYMEVNGTRYF 120

Query: 121 LRQCHWHSPSEHTINGRRFALEAHLVHQSRTGKVAVIGLLYNIGRPDHFLSKLEEYLEDI 180
           L+Q HWHSPSEHTINGRRFALEAHLVHQS TG VAVIG+LY IGRPD+FLSK++EYLE++
Sbjct: 121 LKQFHWHSPSEHTINGRRFALEAHLVHQSSTGSVAVIGVLYKIGRPDYFLSKMKEYLEEV 180

Query: 181 SNSREYDQLNVIDPSLLKMQSSLYYRYIGSLTVPPCSQNVLWTIVREIRTVAPEQVKLLR 240
            +++E   +++ DPS L+M SSLYYRYIGSLTVPPCSQNVLWTIVR+++TV+PEQVKLLR
Sbjct: 181 CDTKEDKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKVKTVSPEQVKLLR 240

Query: 241 VAVHDDSDTNARPLQPLNDRKIQLRIKN 269
           VAVHDDSDTNARPLQPLNDRKIQLRIK+
Sbjct: 241 VAVHDDSDTNARPLQPLNDRKIQLRIKS 268

[Close]

BLAST of CmoCh01G005330.1 vs. TAIR 10
Match: AT1G08080.1 (alpha carbonic anhydrase 7 )

HSP 1 Score: 300.1 bits (767), Expect = 1.8e-81
Identity = 141/238 (59.24%), Postives = 180/238 (75.63%), Query Frame = 0


Query: 28  EVEDQKEFDYDAEGNRGPTHWGDLKREWHGCKTGLMQSPIDLLHKRVRIVSHFTNLKIDY 87
           EVED++EF+Y     +GP  WG+LK EW  C  G MQSPIDL+++RV IVSH   L  DY
Sbjct: 33  EVEDEREFNYKKNDEKGPERWGELKPEWEMCGKGEMQSPIDLMNERVNIVSHLGRLNRDY 92

Query: 88  KPSNATLKNRGHDIMLKWSGGAGSINVNGTRYFLRQCHWHSPSEHTINGRRFALEAHLVH 147
            PSNATLKNRGHDIMLK+  GAG+I +NG  Y L+Q HWHSPSEHTINGRRFALE H+VH
Sbjct: 93  NPSNATLKNRGHDIMLKFEDGAGTIKINGFEYELQQLHWHSPSEHTINGRRFALELHMVH 152

Query: 148 QSRTGKVAVIGLLYNIGRPDHFLSKLEEYLEDISNSREYDQ-LNVIDPSLLKMQSSLYYR 207
           + R  ++AV+ +LY IGR D F+  LE+ LE I+   E ++ + +IDP+ +K+ S  YYR
Sbjct: 153 EGRNRRMAVVTVLYKIGRADTFIRSLEKELEGIAEMEEAEKNVGMIDPTKIKIGSRKYYR 212

Query: 208 YIGSLTVPPCSQNVLWTIVREIRTVAPEQVKLLRVAVHDDSDTNARPLQPLNDRKIQL 265
           Y GSLT PPC+QNV W++VR++RTV  +QVKLLRVAVHDD+++NARP+QP N R + L
Sbjct: 213 YTGSLTTPPCTQNVTWSVVRKVRTVTRKQVKLLRVAVHDDANSNARPVQPTNKRIVHL 270

[Close]

BLAST of CmoCh01G005330.1 vs. TAIR 10
Match: AT1G08065.1 (alpha carbonic anhydrase 5 )

HSP 1 Score: 272.3 bits (695), Expect = 4.1e-73
Identity = 131/242 (54.13%), Postives = 175/242 (72.31%), Query Frame = 0


Query: 28  EVEDQKEFDYDAEGNRGPTHWGDLKREWHGCKTGLMQSPIDLLHKRVRIVSHFTNLKIDY 87
           EVED+ +F+Y+ +G +GP +WG LK EW  C  G MQSPIDL  KRV I  +   L+  Y
Sbjct: 28  EVEDETQFNYEKKGEKGPENWGRLKPEWAMCGKGNMQSPIDLTDKRVLIDHNLGYLRSQY 87

Query: 88  KPSNATLKNRGHDIMLKWSGG-AG-SINVNGTRYFLRQCHWHSPSEHTINGRRFALEAHL 147
            PSNAT+KNRGHDIM+K+ GG AG  I +NGT Y L+Q HWHSPSEHT+NG+RF LE H+
Sbjct: 88  LPSNATIKNRGHDIMMKFEGGNAGLGITINGTEYKLQQIHWHSPSEHTLNGKRFVLEEHM 147

Query: 148 VHQSRTGKVAVIGLLYNIGRPDHFLSKLEEYLEDISNSREYDQ-LNVIDPSLLKMQSSLY 207
           VHQS+ G+ AV+   Y +G+PD+FL  LE YL+ I+++ E  + + ++ P     +S  Y
Sbjct: 148 VHQSKDGRNAVVAFFYKLGKPDYFLLTLERYLKRITDTHESQEFVEMVHPRTFGFESKHY 207

Query: 208 YRYIGSLTVPPCSQNVLWTIVREIRTVAPEQVKLLRVAVHDDSDTNARPLQPLNDRKIQL 267
           YR+IGSLT PPCS+NV+WTI +E+RTV  +Q+ +LRV VHD S++NARPLQ  N+R + L
Sbjct: 208 YRFIGSLTTPPCSENVIWTISKEMRTVTLKQLIMLRVTVHDQSNSNARPLQRKNERPVAL 267

[Close]

BLAST of CmoCh01G005330.1 vs. TAIR 10
Match: AT2G28210.1 (alpha carbonic anhydrase 2 )

HSP 1 Score: 246.1 bits (627), Expect = 3.1e-65
Identity = 118/208 (56.73%), Postives = 151/208 (72.60%), Query Frame = 0


Query: 25  MCQEVEDQKEFDYDAEGNRGPTHWGDLKREWHGCKTGLMQSPIDLLHKRVRIVSHFTNLK 84
           M + VED+ EF Y+     GP  WG L+ EW  C  G MQSPIDL++KRVR+V+H   L 
Sbjct: 1   MDEYVEDEHEFSYEWNQENGPAKWGKLRPEWKMCGKGEMQSPIDLMNKRVRLVTHLKKLT 60

Query: 85  IDYKPSNATLKNRGHDIMLKW-SGGAGSINVNGTRYFLRQCHWHSPSEHTINGRRFALEA 144
             YKP NATLKNRGHD+MLK+   G+GSI VNGT Y L Q HWHSPSEHT+NGRRFALE 
Sbjct: 61  RHYKPCNATLKNRGHDMMLKFGEEGSGSITVNGTEYKLLQLHWHSPSEHTMNGRRFALEL 120

Query: 145 HLVHQSRTGKVAVIGLLYNIGRPDHFLSKLEEYLEDISNSREYDQ-LNVIDPSLLKMQSS 204
           H+VH++  G +AV+ +LY IGRPD FL  LE  L  I++  E ++ ++VIDP  +K+ S 
Sbjct: 121 HMVHENINGSLAVVTVLYKIGRPDSFLGLLENKLSAITDQNEAEKYVDVIDPRDIKIGSR 180

Query: 205 LYYRYIGSLTVPPCSQNVLWTIVREIRT 231
            +YRYIGSLT PPC+QNV+WT+V+++ T
Sbjct: 181 KFYRYIGSLTTPPCTQNVIWTVVKKVNT 208

[Close]

BLAST of CmoCh01G005330.1 vs. TAIR 10
Match: AT4G20990.1 (alpha carbonic anhydrase 4 )

HSP 1 Score: 238.0 bits (606), Expect = 8.6e-63
Identity = 114/257 (44.36%), Postives = 164/257 (63.81%), Query Frame = 0


Query: 9   IFGSFLCGFLLVSWPVMC---QEVEDQKEFDYDAEGNRGPTHWGDLKREWHGCKTGLMQS 68
           IF   +C F+ +S+P +     EV+D+  F Y+ +  +GP  WG +   W  C TG  QS
Sbjct: 8   IFFMAMC-FIYLSFPNISHAHSEVDDETPFTYEQKTEKGPEGWGKINPHWKVCNTGRYQS 67

Query: 69  PIDLLHKRVRIVSHFTNLKIDYKPSNATLKNRGHDIMLKWSGGAGSINVNGTRYFLRQCH 128
           PIDL ++RV ++ H       YKP+ A + NRGHDIM+ W G AG + +  T + L QCH
Sbjct: 68  PIDLTNERVSLI-HDQAWTRQYKPAPAVITNRGHDIMVSWKGDAGKMTIRKTDFNLVQCH 127

Query: 129 WHSPSEHTINGRRFALEAHLVHQSRTGKVAVIGLLYNIGRPDHFLSKLEEYLEDISNSRE 188
           WHSPSEHT+NG R+ LE H+VH S  G+ AVIG+LY +G P+ FL+KL   ++ + N   
Sbjct: 128 WHSPSEHTVNGTRYDLELHMVHTSARGRTAVIGVLYKLGEPNEFLTKLLNGIKAVGNKE- 187

Query: 189 YDQLNVIDPSLLKMQSSLYYRYIGSLTVPPCSQNVLWTIVREIRTVAPEQVKLLRVAVHD 248
              L +IDP  ++ Q+  +YRYIGSLTVPPC++ V+WT+V+ + T++ EQ+  LR AV D
Sbjct: 188 -INLGMIDPREIRFQTRKFYRYIGSLTVPPCTEGVIWTVVKRVNTISMEQITALRQAVDD 247

Query: 249 DSDTNARPLQPLNDRKI 263
             +TN+RP+Q    R +
Sbjct: 248 GFETNSRPVQDSKGRSV 260

[Close]

BLAST of CmoCh01G005330.1 vs. TAIR 10
Match: AT5G56330.1 (alpha carbonic anhydrase 8 )

HSP 1 Score: 218.0 bits (554), Expect = 9.2e-57
Identity = 116/239 (48.54%), Postives = 144/239 (60.25%), Query Frame = 0


Query: 28  EVEDQKEFDYDAEGNRGPTHWGDLKREWHGCKTGLMQSPIDLLHKRVRIVSHFTNLKIDY 87
           EVED+ EF Y+ +GN+GP  WG L  EW  C  G MQSPIDL  K V + + F  L+  Y
Sbjct: 133 EVEDETEFSYETKGNKGPAKWGTLDAEWKMCGIGKMQSPIDLRDKNVVVSNKFGLLRSQY 192

Query: 88  KPSNATLKNRGHDIMLKWSGGAGSINV--NGTRYFLRQCHWHSPSEHTINGRRFALEAHL 147
            PSN T+KNRGHDIMLK+ GG   I V   GTRY L+Q HWHSPSEHTING+RFALE HL
Sbjct: 193 LPSNTTIKNRGHDIMLKFKGGNKGIGVTIRGTRYQLQQLHWHSPSEHTINGKRFALEEHL 252

Query: 148 VHQSRTGKVAVIGLLYNIGRPDHFLSKLEEYLEDISNSREYDQLNVIDPSLLKMQSSLYY 207
           VH+S+  + AV+  LYN+G  D FL  LE+ L+ I+++   ++                 
Sbjct: 253 VHESKDKRYAVVAFLYNLGASDPFLFSLEKQLKKITDTHASEE----------------- 312

Query: 208 RYIGSLTVPPCSQNVLWTIVREIRTVAPEQVKLLRVAVHDDSDTNARPLQPLNDRKIQL 265
                                 IRTV+ +QVKLLRVAVHD SD+NARPLQ +N RK+ L
Sbjct: 313 ---------------------HIRTVSSKQVKLLRVAVHDASDSNARPLQAVNKRKVYL 333

The following BLAST results are available for this feature:

BLAST of CmoCh01G005330.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L817	2.6e-80	59.24	Alpha carbonic anhydrase 7 OS=Arabidopsis thaliana OX=3702 GN=ACA7 PE=2 SV=1	[more]
Q9FM99	9.5e-75	57.08	Alpha carbonic anhydrase 8 OS=Arabidopsis thaliana OX=3702 GN=ACA8 PE=3 SV=3	[more]
F4HUC4	5.8e-72	54.13	Alpha carbonic anhydrase 5 OS=Arabidopsis thaliana OX=3702 GN=ACA5 PE=3 SV=1	[more]
Q84UV8	1.9e-67	50.19	Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3 OS...	[more]
F4IHR4	8.9e-65	53.78	Alpha carbonic anhydrase 2 OS=Arabidopsis thaliana OX=3702 GN=ACA2 PE=2 SV=2	[more]

BLAST of CmoCh01G005330.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G941	1.8e-156	100.00	Carbonic anhydrase OS=Cucurbita moschata OX=3662 GN=LOC111452083 PE=3 SV=1	[more]
A0A6J1KFK2	4.8e-154	98.13	Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111493412 PE=3 SV=1	[more]
A0A6J1HYT6	5.5e-126	80.97	Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111469328 PE=3 SV=1	[more]
A0A6J1I3K8	8.0e-125	79.85	Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111469329 PE=3 SV=1	[more]
A0A6J1HKB2	2.6e-123	77.99	Carbonic anhydrase OS=Cucurbita moschata OX=3662 GN=LOC111464883 PE=3 SV=1	[more]

BLAST of CmoCh01G005330.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022948392.1	3.6e-156	100.00	alpha carbonic anhydrase 7-like [Cucurbita moschata] >KAG6607147.1 Alpha carboni...	[more]
XP_022998889.1	9.9e-154	98.13	alpha carbonic anhydrase 7-like [Cucurbita maxima]	[more]
XP_023523228.1	1.4e-152	97.76	alpha carbonic anhydrase 7-like [Cucurbita pepo subsp. pepo]	[more]
XP_023519286.1	3.9e-126	79.85	alpha carbonic anhydrase 7-like [Cucurbita pepo subsp. pepo] >XP_023519287.1 alp...	[more]
KAG7020784.1	5.1e-126	80.22	Alpha carbonic anhydrase 7, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of CmoCh01G005330.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G08080.1	1.8e-81	59.24	alpha carbonic anhydrase 7	[more]
AT1G08065.1	4.1e-73	54.13	alpha carbonic anhydrase 5	[more]
AT2G28210.1	3.1e-65	56.73	alpha carbonic anhydrase 2	[more]
AT4G20990.1	8.6e-63	44.36	alpha carbonic anhydrase 4	[more]
AT5G56330.1	9.2e-57	48.54	alpha carbonic anhydrase 8	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001148	Alpha carbonic anhydrase domain	SMART	SM01057	Carb_anhydrase_2a	coord: 37..266 e-value: 2.2E-72 score: 256.4
IPR001148	Alpha carbonic anhydrase domain	PFAM	PF00194	Carb_anhydrase	coord: 36..263 e-value: 2.2E-41 score: 142.1
IPR001148	Alpha carbonic anhydrase domain	PROSITE	PS51144	ALPHA_CA_2	coord: 33..266 score: 46.668339
IPR036398	Alpha carbonic anhydrase domain superfamily	GENE3D	3.10.200.10	Alpha carbonic anhydrase	coord: 31..267 e-value: 1.9E-75 score: 255.4
IPR036398	Alpha carbonic anhydrase domain superfamily	SUPERFAMILY	51069	Carbonic anhydrase	coord: 34..262
None	No IPR available	PANTHER	PTHR18952:SF242	ALPHA CARBONIC ANHYDRASE-RELATED	coord: 23..264
IPR023561	Carbonic anhydrase, alpha-class	PANTHER	PTHR18952	CARBONIC ANHYDRASE	coord: 23..264
IPR018338	Carbonic anhydrase, alpha-class, conserved site	PROSITE	PS00162	ALPHA_CA_1	coord: 130..146
IPR041891	Carbonic anhydrase, prokaryotic-like	CDD	cd03124	alpha_CA_prokaryotic_like	coord: 44..264 e-value: 3.46442E-93 score: 271.838

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmoCh01G005330	CmoCh01G005330	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh01G005330.1:exon:2866	CmoCh01G005330.1:exon:2866	exon
CmoCh01G005330.1:exon:2867	CmoCh01G005330.1:exon:2867	exon
CmoCh01G005330.1:exon:2868	CmoCh01G005330.1:exon:2868	exon
CmoCh01G005330.1:exon:2869	CmoCh01G005330.1:exon:2869	exon
CmoCh01G005330.1:exon:2870	CmoCh01G005330.1:exon:2870	exon
CmoCh01G005330.1:exon:2871	CmoCh01G005330.1:exon:2871	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh01G005330.1:five_prime_utr	CmoCh01G005330.1:five_prime_utr	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh01G005330.1:cds	CmoCh01G005330.1:cds	CDS
CmoCh01G005330.1:cds	CmoCh01G005330.1:cds_2	CDS
CmoCh01G005330.1:cds	CmoCh01G005330.1:cds_3	CDS
CmoCh01G005330.1:cds	CmoCh01G005330.1:cds_4	CDS
CmoCh01G005330.1:cds	CmoCh01G005330.1:cds_5	CDS
CmoCh01G005330.1:cds	CmoCh01G005330.1:cds_6	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh01G005330.1:three_prime_utr	CmoCh01G005330.1:three_prime_utr	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmoCh01G005330.1	CmoCh01G005330.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006730	one-carbon metabolic process
cellular_component	GO:0009570	chloroplast stroma
molecular_function	GO:0004089	carbonate dehydratase activity
molecular_function	GO:0008270	zinc ion binding