CmaCh19G008520.1 (mRNA) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh19G008520.1
Type	mRNA
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
Location	Cma_Chr19: 8010532 .. 8011836 (+)
Sequence length	1305
RNA-Seq Expression	CmaCh19G008520.1
Synteny	CmaCh19G008520.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTATTAATCAGAAACCTCGCCTCAAAGATTCTGCTCTGAGACGTTTTAAATTGGTTATTCTTGTGACATTATTGGGGTTCTTGCTGTTTATGATCCTTATGGAGACGAATTTGTTTCAGAATTTTGGTCCTTGGTTTTCTGATAATATTCGTGAGGATCCATATGCTGGCTTTGGTCGTTTTGATTCTAATGATGTACTTAAGCTGCCCAAACAAACTGAGTTATCGGTTCGTTTAGAAAAGCGAAATCGATTGCCACTTCGAAACTTAGATCTGTATCCAAATCTAGCCAAGGATCATATACCTATTGTACTTTATGTGCACAATCGTCCGCAGTATCTTCGAGTTGTAATCGATAGCTTGTCCAAAGTTACAGGAATAAGTGAAACCTTGCTAATTGTTAGCCATGATGGGTACTTTGAAGAAATGGACAAGCTTGTCCAGAACATCAGGTTTTGTTCAGTGAAACAGATTTTTGCTCCATACTCTCCTCATCTCTTTCCCAACAGCTTTCCCGGTGTCTCGCCTACAGACTGTAAGGGGAAACATTATCCTATGATAACAACAAATTGCAGAGGGAATCCTGATCAGTATGGAAACCATCGGTTGCCTAAAATCGTTTCGTTAAAGCACCATTGGTGGTGGATGATGAATACGGTGTGGGATGGATTGGAGGAGACGCGGGGGTTGTCGGGTCATATTTTGTTTATTGAGGAAGATCATTTTATATTGCCAAATGCGTATCGGAATCTGCAGTTACTCGTGACGTTGAAGCCTAAAAAATGTCCTGAATGTCATGCTGTGAATCTAGCTCCTAGTGATGTGAAATCAAGAGGTGAGGGTAACAATTTTCTCGTTGCAGAGAGGATGGGAAATATTGGTTATGCTTTCAATAGGTCTGTTTGGAAGAAAATCCATAAGAAAGCAAAGGAGTTCTGTTTTTTTGATGAATACAATTGGGATATAACAATGTGGGCAACCGTTTTTCCCTCGTTTGGCAGCCCTGTTTATTCACTTCGAGGCCCTTGGACGAGCGCAATTCACTTTGGCAAATGTGGTTTACACCAGGGTCAGGACGAGGGGGATGTTTGTATTGATAACGGTTTGTTAAACATCGACGTTAAAGATATCGACGAAGTTGTGAACATTAATTCGGAGTGGCCAGTCGAAGTCTTCAGAGGTCAGCCTGGCTATGGAGCTGGGTTCAGGGGTTGGGGAGGTTGGGGTGATGAAAGAGACCGTCAGTTGTGTCTAAATTTTGCCCAAATGTATCGTTCCATGCCGAACGTATCCCGTTTGTGA

mRNA sequence

ATGGCTATTAATCAGAAACCTCGCCTCAAAGATTCTGCTCTGAGACGTTTTAAATTGGTTATTCTTGTGACATTATTGGGGTTCTTGCTGTTTATGATCCTTATGGAGACGAATTTGTTTCAGAATTTTGGTCCTTGGTTTTCTGATAATATTCGTGAGGATCCATATGCTGGCTTTGGTCGTTTTGATTCTAATGATGTACTTAAGCTGCCCAAACAAACTGAGTTATCGGTTCGTTTAGAAAAGCGAAATCGATTGCCACTTCGAAACTTAGATCTGTATCCAAATCTAGCCAAGGATCATATACCTATTGTACTTTATGTGCACAATCGTCCGCAGTATCTTCGAGTTGTAATCGATAGCTTGTCCAAAGTTACAGGAATAAGTGAAACCTTGCTAATTGTTAGCCATGATGGGTACTTTGAAGAAATGGACAAGCTTGTCCAGAACATCAGGTTTTGTTCAGTGAAACAGATTTTTGCTCCATACTCTCCTCATCTCTTTCCCAACAGCTTTCCCGGTGTCTCGCCTACAGACTGTAAGGGGAAACATTATCCTATGATAACAACAAATTGCAGAGGGAATCCTGATCAGTATGGAAACCATCGGTTGCCTAAAATCGTTTCGTTAAAGCACCATTGGTGGTGGATGATGAATACGGTGTGGGATGGATTGGAGGAGACGCGGGGGTTGTCGGGTCATATTTTGTTTATTGAGGAAGATCATTTTATATTGCCAAATGCGTATCGGAATCTGCAGTTACTCGTGACGTTGAAGCCTAAAAAATGTCCTGAATGTCATGCTGTGAATCTAGCTCCTAGTGATGTGAAATCAAGAGGTGAGGGTAACAATTTTCTCGTTGCAGAGAGGATGGGAAATATTGGTTATGCTTTCAATAGGTCTGTTTGGAAGAAAATCCATAAGAAAGCAAAGGAGTTCTGTTTTTTTGATGAATACAATTGGGATATAACAATGTGGGCAACCGTTTTTCCCTCGTTTGGCAGCCCTGTTTATTCACTTCGAGGCCCTTGGACGAGCGCAATTCACTTTGGCAAATGTGGTTTACACCAGGGTCAGGACGAGGGGGATGTTTGTATTGATAACGGTTTGTTAAACATCGACGTTAAAGATATCGACGAAGTTGTGAACATTAATTCGGAGTGGCCAGTCGAAGTCTTCAGAGGTCAGCCTGGCTATGGAGCTGGGTTCAGGGGTTGGGGAGGTTGGGGTGATGAAAGAGACCGTCAGTTGTGTCTAAATTTTGCCCAAATGTATCGTTCCATGCCGAACGTATCCCGTTTGTGA

Coding sequence (CDS)

ATGGCTATTAATCAGAAACCTCGCCTCAAAGATTCTGCTCTGAGACGTTTTAAATTGGTTATTCTTGTGACATTATTGGGGTTCTTGCTGTTTATGATCCTTATGGAGACGAATTTGTTTCAGAATTTTGGTCCTTGGTTTTCTGATAATATTCGTGAGGATCCATATGCTGGCTTTGGTCGTTTTGATTCTAATGATGTACTTAAGCTGCCCAAACAAACTGAGTTATCGGTTCGTTTAGAAAAGCGAAATCGATTGCCACTTCGAAACTTAGATCTGTATCCAAATCTAGCCAAGGATCATATACCTATTGTACTTTATGTGCACAATCGTCCGCAGTATCTTCGAGTTGTAATCGATAGCTTGTCCAAAGTTACAGGAATAAGTGAAACCTTGCTAATTGTTAGCCATGATGGGTACTTTGAAGAAATGGACAAGCTTGTCCAGAACATCAGGTTTTGTTCAGTGAAACAGATTTTTGCTCCATACTCTCCTCATCTCTTTCCCAACAGCTTTCCCGGTGTCTCGCCTACAGACTGTAAGGGGAAACATTATCCTATGATAACAACAAATTGCAGAGGGAATCCTGATCAGTATGGAAACCATCGGTTGCCTAAAATCGTTTCGTTAAAGCACCATTGGTGGTGGATGATGAATACGGTGTGGGATGGATTGGAGGAGACGCGGGGGTTGTCGGGTCATATTTTGTTTATTGAGGAAGATCATTTTATATTGCCAAATGCGTATCGGAATCTGCAGTTACTCGTGACGTTGAAGCCTAAAAAATGTCCTGAATGTCATGCTGTGAATCTAGCTCCTAGTGATGTGAAATCAAGAGGTGAGGGTAACAATTTTCTCGTTGCAGAGAGGATGGGAAATATTGGTTATGCTTTCAATAGGTCTGTTTGGAAGAAAATCCATAAGAAAGCAAAGGAGTTCTGTTTTTTTGATGAATACAATTGGGATATAACAATGTGGGCAACCGTTTTTCCCTCGTTTGGCAGCCCTGTTTATTCACTTCGAGGCCCTTGGACGAGCGCAATTCACTTTGGCAAATGTGGTTTACACCAGGGTCAGGACGAGGGGGATGTTTGTATTGATAACGGTTTGTTAAACATCGACGTTAAAGATATCGACGAAGTTGTGAACATTAATTCGGAGTGGCCAGTCGAAGTCTTCAGAGGTCAGCCTGGCTATGGAGCTGGGTTCAGGGGTTGGGGAGGTTGGGGTGATGAAAGAGACCGTCAGTTGTGTCTAAATTTTGCCCAAATGTATCGTTCCATGCCGAACGTATCCCGTTTGTGA

Protein sequence

MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFGPWFSDNIREDPYAGFGRFDSNDVLKLPKQTELSVRLEKRNRLPLRNLDLYPNLAKDHIPIVLYVHNRPQYLRVVIDSLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPNSFPGVSPTDCKGKHYPMITTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEEDHFILPNAYRNLQLLVTLKPKKCPECHAVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNRSVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQDEGDVCIDNGLLNIDVKDIDEVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLNFAQMYRSMPNVSRL

Homology

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BLAST of CmaCh19G008520.1 vs. ExPASy Swiss-Prot
Match: Q9FT88 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Arabidopsis thaliana OX=3702 GN=GNT2 PE=1 SV=1)

HSP 1 Score: 568.2 bits (1463), Expect = 8.2e-161
Identity = 272/423 (64.30%), Postives = 328/423 (77.54%), Query Frame = 0


Query: 5   QKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFGPWFSDNIREDPYAGFGRFDS 64
           +K RL+D+ L R  ++  VTL   L+ + +  T L  +      D + +D  A      +
Sbjct: 6   KKQRLRDTGLCRLGILFAVTLSIVLMLVSVPRTALNGSSIDDDLDGLDKDLEAKL----N 65

Query: 65  NDVLKLPKQTELSVRLEKRNRLPLRNLDLYPNLAKDHIPIVLYVHNRPQYLRVVIDSLSK 124
             +L + +   +S+RL +RN    RN DL+P+LAKD + IVLYVHNR QY RV ++SLSK
Sbjct: 66  ASLLSVARGNRMSLRLHRRNHFSPRNTDLFPDLAKDRVVIVLYVHNRAQYFRVTVESLSK 125

Query: 125 VTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPNSFPGVSPTDCKGKH 184
           V GISETLLIVSHDGYFEEM+++V++I+FC VKQIF+PYSPH++  SFPGV+  DCK K 
Sbjct: 126 VKGISETLLIVSHDGYFEEMNRIVESIKFCQVKQIFSPYSPHIYRTSFPGVTLNDCKNKG 185

Query: 185 YPMITTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEEDHFI 244
                 +C GNPDQYGNHR PKIVSLKHHWWWMMNTVWDGLEET+G  GHILFIEEDHF+
Sbjct: 186 -DEAKGHCEGNPDQYGNHRSPKIVSLKHHWWWMMNTVWDGLEETKGHEGHILFIEEDHFL 245

Query: 245 LPNAYRNLQLLVTLKPKKCPECHAVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNRSVWK 304
            PNAYRN+Q L  LKP KCP+C A NLAPSDVKSRGEG   LVAERMGN+GY+FNRSVW+
Sbjct: 246 FPNAYRNIQTLTRLKPAKCPDCFAANLAPSDVKSRGEGLESLVAERMGNVGYSFNRSVWE 305

Query: 305 KIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQ-DEGD 364
            IH+KA+EFCFFD+YNWDITMWATVFPSFGSPVY+LRGP TSA+HFGKCGLHQG+ DEGD
Sbjct: 306 NIHQKAREFCFFDDYNWDITMWATVFPSFGSPVYTLRGPRTSAVHFGKCGLHQGRGDEGD 365

Query: 365 VCIDNGLLNIDVKDIDEVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLNFAQ 424
            CIDNG++NI+VK+ D+VVNI   W V V++ Q GY AGF GWGGWGD+RDR LCL+FA 
Sbjct: 366 -CIDNGVVNIEVKETDKVVNIKEGWGVRVYKHQAGYKAGFEGWGGWGDDRDRHLCLDFAT 422

Query: 425 MYR 427
           MYR
Sbjct: 426 MYR 422

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BLAST of CmaCh19G008520.1 vs. ExPASy Swiss-Prot
Match: Q10469 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Homo sapiens OX=9606 GN=MGAT2 PE=1 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 4.5e-50
Identity = 120/355 (33.80%), Postives = 184/355 (51.83%), Query Frame = 0


Query: 88  LRNLDLYPNLAKDHIPIVLYVHNRPQYLRVVIDSLSKVTGISETLLIVSHDGYFEEMDKL 147
           LRN+D     A   + +V+ VHNRP+YLR+++DSL K  GI   L+I SHD +  E+++L
Sbjct: 104 LRNVDKAGTWAPRELVLVVQVHNRPEYLRLLLDSLRKAQGIDNVLVIFSHDFWSTEINQL 163

Query: 148 VQNIRFCSVKQIFAPYSPHLFPNSFPGVSPTDC-----KGKHYPMITTNCRGNPDQYGNH 207
           +  + FC V Q+F P+S  L+PN FPG  P DC     K     +   N    PD +G++
Sbjct: 164 IAGVNFCPVLQVFFPFSIQLYPNEFPGSDPRDCPRDLPKNAALKLGCINAE-YPDSFGHY 223

Query: 208 RLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEEDHFILPNAYRNLQLLVTLKPKK 267
           R  K    KHHWWW ++ VW+ ++  R  +G ILF+EEDH++ P+ Y   + +  LK ++
Sbjct: 224 REAKFSQTKHHWWWKLHFVWERVKILRDYAGLILFLEEDHYLAPDFYHVFKKMWKLKQQE 283

Query: 268 CPECHAVNLAPSDVKSRGEGNNFLVAERM-----GNIGYAFNRSVWKKIHKKAKEFCFFD 327
           CPEC  ++L          G    V  +       N+G A  R+ ++K+ +    FC +D
Sbjct: 284 CPECDVLSLGTYSASRSFYGMADKVDVKTWKSTEHNMGLALTRNAYQKLIECTDTFCTYD 343

Query: 328 EYNWDITM-WATV--FPSFGSPVYSLRGPWTSAI-HFGKCGLHQGQ----DEGDVCIDNG 387
           +YNWD T+ + TV   P F    + +  P    I H G CG+H  +          I++ 
Sbjct: 344 DYNWDWTLQYLTVSCLPKF----WKVLVPQIPRIFHAGDCGMHHKKTCRPSTQSAQIESL 403

Query: 388 LLNIDVKDIDEVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLNFAQM 425
           L N       E + I+ ++ V V    P      R  GGWGD RD +LC ++ ++
Sbjct: 404 LNNNKQYMFPETLTISEKFTV-VAISPP------RKNGGWGDIRDHELCKSYRRL 446

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BLAST of CmaCh19G008520.1 vs. ExPASy Swiss-Prot
Match: O19071 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Sus scrofa OX=9823 GN=MGAT2 PE=3 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 2.9e-49
Identity = 115/357 (32.21%), Postives = 184/357 (51.54%), Query Frame = 0


Query: 88  LRNLDLYPNLAKDHIPIVLYVHNRPQYLRVVIDSLSKVTGISETLLIVSHDGYFEEMDKL 147
           LRN+D   +     + +V+ VHNR +YL++++DSL K  GI   L+I SHD +  E+++L
Sbjct: 104 LRNVDKVSSWVPRELVLVVQVHNRAEYLKLLLDSLRKAQGIDNVLVIFSHDFWSTEINQL 163

Query: 148 VQNIRFCSVKQIFAPYSPHLFPNSFPGVSPTDC-----KGKHYPMITTNCRGNPDQYGNH 207
           +  + FC V Q+F P+S  L+PN FPG  P DC     K     M   N    PD +G++
Sbjct: 164 IAGVDFCPVLQVFFPFSIQLYPNEFPGTDPRDCPRDLEKNAALKMGCINAE-YPDSFGHY 223

Query: 208 RLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEEDHFILPNAYRNLQLLVTLKPKK 267
           R  K    KHHWWW ++ VW+ ++  R  +G ILF+EEDH++ P+ Y   + +  LK ++
Sbjct: 224 REAKFSQTKHHWWWKLHFVWERVKVLRDYAGLILFLEEDHYVAPDFYHVFKKMWNLKQQE 283

Query: 268 CPECHAVNLAP-------SDVKSRGEGNNFLVAERMGNIGYAFNRSVWKKIHKKAKEFCF 327
           CPEC  ++L          DV  + +   +   E   N+G A  R  ++K+ +    FC 
Sbjct: 284 CPECDVLSLGTYTTVRSFRDVADKVDVKTWKSTEH--NMGLALTRDAYQKLIECTDTFCT 343

Query: 328 FDEYNWDITM-WATV--FPSFGSPVYSLRGPWTSAI-HFGKCGLHQGQDEGDVCIDNGLL 387
           +D+YNWD T+ + TV   P F    + +  P    I H G CG+H  +     C      
Sbjct: 344 YDDYNWDWTLQYLTVSCLPKF----WKVLVPQVPRIFHAGDCGMHHKK----TCRP---- 403

Query: 388 NIDVKDIDEVVNINSEW----PVEVFRGQPGYGAGFRGWGGWGDERDRQLCLNFAQM 425
           +     I+ ++N N ++     + +        +  R  GGWGD RD +LC ++ ++
Sbjct: 404 STQSAQIESLLNSNKQYMFPETLTISEKLTAALSPPRKNGGWGDIRDHELCKSYRRL 445

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BLAST of CmaCh19G008520.1 vs. ExPASy Swiss-Prot
Match: Q09326 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Rattus norvegicus OX=10116 GN=Mgat2 PE=1 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 8.5e-49
Identity = 113/355 (31.83%), Postives = 180/355 (50.70%), Query Frame = 0


Query: 88  LRNLDLYPNLAKDHIPIVLYVHNRPQYLRVVIDSLSKVTGISETLLIVSHDGYFEEMDKL 147
           LRN+D     +   + +V+ VHNRP+YLR++IDSL K  GI E L+I SHD +  E++ L
Sbjct: 99  LRNVDKDGTWSPGELVLVVQVHNRPEYLRLLIDSLRKAQGIREVLVIFSHDFWSAEINSL 158

Query: 148 VQNIRFCSVKQIFAPYSPHLFPNSFPGVSPTDCKG--KHYPMITTNC--RGNPDQYGNHR 207
           + ++ FC V Q+F P+S  L+P+ FPG  P DC    K    +   C     PD +G++R
Sbjct: 159 ISSVDFCPVLQVFFPFSIQLYPSEFPGSDPRDCPRDLKKNAALKLGCINAEYPDSFGHYR 218

Query: 208 LPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEEDHFILPNAYRNLQLLVTLKPKKC 267
             K    KHHWWW ++ VW+ ++  +  +G ILF+EEDH++ P+ Y   + +  LK ++C
Sbjct: 219 EAKFSQTKHHWWWKLHFVWERVKVLQDYTGLILFLEEDHYLAPDFYHVFKKMWKLKQQEC 278

Query: 268 PECHAVNLAPSDVKSRGEGNNFLVAERMG---------NIGYAFNRSVWKKIHKKAKEFC 327
           P C  ++L          G    +A+++          N+G A  R  ++K+ +    FC
Sbjct: 279 PGCDVLSLGTYTTIRSFYG----IADKVDVKTWKSTEHNMGLALTRDAYQKLIECTDTFC 338

Query: 328 FFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAI-HFGKCGLHQGQ----DEGDVCIDNG 387
            +D+YNWD T+           V+ +  P    I H G CG+H  +          I++ 
Sbjct: 339 TYDDYNWDWTLQYLTLACL-PKVWKVLVPQAPRIFHAGDCGMHHKKTCRPSTQSAQIESL 398

Query: 388 LLNIDVKDIDEVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLNFAQM 425
           L N       E + I  ++P+             R  GGWGD RD +LC ++ ++
Sbjct: 399 LNNNKQYLFPETLVIGEKFPMAAISPP-------RKNGGWGDIRDHELCKSYRRL 441

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BLAST of CmaCh19G008520.1 vs. ExPASy Swiss-Prot
Match: Q921V5 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Mus musculus OX=10090 GN=Mgat2 PE=1 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 2.5e-48
Identity = 113/356 (31.74%), Postives = 181/356 (50.84%), Query Frame = 0


Query: 88  LRNLDLYPNLAKDHIPIVLYVHNRPQYLRVVIDSLSKVTGISETLLIVSHDGYFEEMDKL 147
           LRN+      +   + +V+ VHNRP+YLR++IDSL K  GI E L+I SHD +  E++ L
Sbjct: 99  LRNVGNDGTWSPGELVLVVQVHNRPEYLRLLIDSLRKAQGIQEVLVIFSHDFWSAEINSL 158

Query: 148 VQNIRFCSVKQIFAPYSPHLFPNSFPGVSPTDCKG--KHYPMITTNC--RGNPDQYGNHR 207
           +  + FC V Q+F P+S  L+PN FPG  P DC    K    +   C     PD +G++R
Sbjct: 159 ISRVDFCPVLQVFFPFSIQLYPNEFPGSDPRDCPRDLKKNAALKLGCINAEYPDSFGHYR 218

Query: 208 LPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEEDHFILPNAYRNLQLLVTLKPKKC 267
             K    KHHWWW ++ VW+ ++  +  +G ILF+EEDH++ P+ Y   + +  LK ++C
Sbjct: 219 EAKFSQTKHHWWWKLHFVWERVKVLQDYTGLILFLEEDHYLAPDFYHVFKKMWKLKQQEC 278

Query: 268 PECHAVNLAPSDVKSRGEGNNFLVAERMG---------NIGYAFNRSVWKKIHKKAKEFC 327
           P C  ++L          G    +A+++          N+G A  R  ++K+ +    FC
Sbjct: 279 PGCDVLSLGTYTTIRSFYG----IADKVDVKTWKSTEHNMGLALTRDAYQKLIECTDTFC 338

Query: 328 FFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAI-HFGKCGLHQGQDEGDVCIDNGLLNI 387
            +D+YNWD T+           ++ +  P    I H G CG+H  +     C      + 
Sbjct: 339 TYDDYNWDWTLQYLTLACL-PKIWKVLVPQAPRIFHAGDCGMHHKK----TCRP----ST 398

Query: 388 DVKDIDEVVNINSEW--PVEVFRGQPGYGAGF---RGWGGWGDERDRQLCLNFAQM 425
               I+ ++N N ++  P  +  G+    A     R  GGWGD RD +LC ++ ++
Sbjct: 399 QSAQIESLLNSNKQYLFPETLVIGEKFPMAAISPPRKNGGWGDIRDHELCKSYRRL 441

[Close]

BLAST of CmaCh19G008520.1 vs. ExPASy TrEMBL
Match: A0A6J1HWS5 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111468194 PE=3 SV=1)

HSP 1 Score: 919.5 bits (2375), Expect = 5.4e-264
Identity = 434/434 (100.00%), Postives = 434/434 (100.00%), Query Frame = 0


Query: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFGPWFSDNIREDPYAGFG 60
           MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFGPWFSDNIREDPYAGFG
Sbjct: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFGPWFSDNIREDPYAGFG 60

Query: 61  RFDSNDVLKLPKQTELSVRLEKRNRLPLRNLDLYPNLAKDHIPIVLYVHNRPQYLRVVID 120
           RFDSNDVLKLPKQTELSVRLEKRNRLPLRNLDLYPNLAKDHIPIVLYVHNRPQYLRVVID
Sbjct: 61  RFDSNDVLKLPKQTELSVRLEKRNRLPLRNLDLYPNLAKDHIPIVLYVHNRPQYLRVVID 120

Query: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPNSFPGVSPTDC 180
           SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPNSFPGVSPTDC
Sbjct: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPNSFPGVSPTDC 180

Query: 181 KGKHYPMITTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240
           KGKHYPMITTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE
Sbjct: 181 KGKHYPMITTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240

Query: 241 DHFILPNAYRNLQLLVTLKPKKCPECHAVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300
           DHFILPNAYRNLQLLVTLKPKKCPECHAVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR
Sbjct: 241 DHFILPNAYRNLQLLVTLKPKKCPECHAVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300

Query: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD 360
           SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD
Sbjct: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD 360

Query: 361 EGDVCIDNGLLNIDVKDIDEVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420
           EGDVCIDNGLLNIDVKDIDEVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN
Sbjct: 361 EGDVCIDNGLLNIDVKDIDEVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420

Query: 421 FAQMYRSMPNVSRL 435
           FAQMYRSMPNVSRL
Sbjct: 421 FAQMYRSMPNVSRL 434

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BLAST of CmaCh19G008520.1 vs. ExPASy TrEMBL
Match: A0A6J1GLB7 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111455025 PE=3 SV=1)

HSP 1 Score: 891.3 bits (2302), Expect = 1.6e-255
Identity = 419/433 (96.77%), Postives = 426/433 (98.38%), Query Frame = 0


Query: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFGPWFSDNIREDPYAGFG 60
           MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNF PW SDNIREDPYAGFG
Sbjct: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFAPWSSDNIREDPYAGFG 60

Query: 61  RFDSNDVLKLPKQTELSVRLEKRNRLPLRNLDLYPNLAKDHIPIVLYVHNRPQYLRVVID 120
           RFDSND LKLPKQTELS+RLEKRNRLPLRNLDLYPNLAKDHIPIVLYVHNRPQYLRVVID
Sbjct: 61  RFDSNDELKLPKQTELSIRLEKRNRLPLRNLDLYPNLAKDHIPIVLYVHNRPQYLRVVID 120

Query: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPNSFPGVSPTDC 180
           SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFP+SFPGVSPTDC
Sbjct: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDC 180

Query: 181 KGKHYPMITTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240
            GKHYPM+TTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE
Sbjct: 181 MGKHYPMLTTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240

Query: 241 DHFILPNAYRNLQLLVTLKPKKCPECHAVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300
           DHFILPNAYRNLQ LV+LKPKKCPEC++VNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR
Sbjct: 241 DHFILPNAYRNLQFLVSLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300

Query: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD 360
           SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGS VYSLRGPWTSAIHFGKCGLHQGQD
Sbjct: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSSVYSLRGPWTSAIHFGKCGLHQGQD 360

Query: 361 EGDVCIDNGLLNIDVKDIDEVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420
           EGDVCIDNGLLNIDVKDID+VVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN
Sbjct: 361 EGDVCIDNGLLNIDVKDIDKVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420

Query: 421 FAQMYRSMPNVSR 434
           FAQMYRS PNVSR
Sbjct: 421 FAQMYRSTPNVSR 433

[Close]

BLAST of CmaCh19G008520.1 vs. ExPASy TrEMBL
Match: A0A5A7UTF0 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold295G00360 PE=3 SV=1)

HSP 1 Score: 786.9 bits (2031), Expect = 4.2e-224
Identity = 366/430 (85.12%), Postives = 395/430 (91.86%), Query Frame = 0


Query: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFGPWFSDNIREDPYAGFG 60
           MAINQKPRLKD+AL+RFKLV+ VTLLG LLFMIL ETN   NF  W SD+I EDPY GFG
Sbjct: 1   MAINQKPRLKDAALKRFKLVVFVTLLGLLLFMILTETNSSWNFAAWSSDDIDEDPYLGFG 60

Query: 61  RFDSNDVLKLPKQTELSVRLEKRNRLPLRNLDLYPNLAKDHIPIVLYVHNRPQYLRVVID 120
            FDSN  L+LPKQ+ELS+RLEKRNRLP RNLDLYP LAK+HIPIVLYVHNRPQYLRVV+D
Sbjct: 61  HFDSNVELRLPKQSELSIRLEKRNRLPPRNLDLYPKLAKNHIPIVLYVHNRPQYLRVVVD 120

Query: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPNSFPGVSPTDC 180
           SL+KV GISETLL+VSHDGYFEEMDKLVQ+IRFC VKQIFAPYSPHLFPNSFPGVSP DC
Sbjct: 121 SLAKVIGISETLLVVSHDGYFEEMDKLVQSIRFCQVKQIFAPYSPHLFPNSFPGVSPADC 180

Query: 181 KGKHYPMITTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240
           KGK  P    +CRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGL+ET+G+SGHILFIEE
Sbjct: 181 KGKDDPK-AVHCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKETQGMSGHILFIEE 240

Query: 241 DHFILPNAYRNLQLLVTLKPKKCPECHAVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300
           DHFILPNAYRNLQLLVTLKPKKCPEC++VNLAPSDVKSRGEG+NFLVAERMGNIGYAFNR
Sbjct: 241 DHFILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSRGEGSNFLVAERMGNIGYAFNR 300

Query: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD 360
           +VW+KIHK+AKEFCFFDEYNWDITMWATV+PSFG PVYSLRG W SA+HFGKCGLHQGQ 
Sbjct: 301 TVWRKIHKQAKEFCFFDEYNWDITMWATVYPSFGKPVYSLRGTWASAVHFGKCGLHQGQG 360

Query: 361 EGDVCIDNGLLNIDVKDIDEVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420
           + DVCIDNG+LNIDVKDID+V NINSEW V+VFR QPGYGAGFRGWGGWGDERDRQLCLN
Sbjct: 361 DRDVCIDNGVLNIDVKDIDKVANINSEWRVDVFRDQPGYGAGFRGWGGWGDERDRQLCLN 420

Query: 421 FAQMYRSMPN 431
           FA MY S+PN
Sbjct: 421 FAHMYHSVPN 429

[Close]

BLAST of CmaCh19G008520.1 vs. ExPASy TrEMBL
Match: A0A1S3CG23 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucumis melo OX=3656 GN=LOC103500510 PE=3 SV=1)

HSP 1 Score: 786.9 bits (2031), Expect = 4.2e-224
Identity = 366/430 (85.12%), Postives = 395/430 (91.86%), Query Frame = 0


Query: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFGPWFSDNIREDPYAGFG 60
           MAINQKPRLKD+AL+RFKLV+ VTLLG LLFMIL ETN   NF  W SD+I EDPY GFG
Sbjct: 1   MAINQKPRLKDAALKRFKLVVFVTLLGLLLFMILTETNSSWNFAAWSSDDIDEDPYLGFG 60

Query: 61  RFDSNDVLKLPKQTELSVRLEKRNRLPLRNLDLYPNLAKDHIPIVLYVHNRPQYLRVVID 120
            FDSN  L+LPKQ+ELS+RLEKRNRLP RNLDLYP LAK+HIPIVLYVHNRPQYLRVV+D
Sbjct: 61  HFDSNVELRLPKQSELSIRLEKRNRLPPRNLDLYPKLAKNHIPIVLYVHNRPQYLRVVVD 120

Query: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPNSFPGVSPTDC 180
           SL+KV GISETLL+VSHDGYFEEMDKLVQ+IRFC VKQIFAPYSPHLFPNSFPGVSP DC
Sbjct: 121 SLAKVIGISETLLVVSHDGYFEEMDKLVQSIRFCQVKQIFAPYSPHLFPNSFPGVSPADC 180

Query: 181 KGKHYPMITTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240
           KGK  P    +CRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGL+ET+G+SGHILFIEE
Sbjct: 181 KGKDDPK-AVHCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKETQGMSGHILFIEE 240

Query: 241 DHFILPNAYRNLQLLVTLKPKKCPECHAVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300
           DHFILPNAYRNLQLLVTLKPKKCPEC++VNLAPSDVKSRGEG+NFLVAERMGNIGYAFNR
Sbjct: 241 DHFILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSRGEGSNFLVAERMGNIGYAFNR 300

Query: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD 360
           +VW+KIHK+AKEFCFFDEYNWDITMWATV+PSFG PVYSLRG W SA+HFGKCGLHQGQ 
Sbjct: 301 TVWRKIHKQAKEFCFFDEYNWDITMWATVYPSFGKPVYSLRGTWASAVHFGKCGLHQGQG 360

Query: 361 EGDVCIDNGLLNIDVKDIDEVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420
           + DVCIDNG+LNIDVKDID+V NINSEW V+VFR QPGYGAGFRGWGGWGDERDRQLCLN
Sbjct: 361 DRDVCIDNGVLNIDVKDIDKVANINSEWRVDVFRDQPGYGAGFRGWGGWGDERDRQLCLN 420

Query: 421 FAQMYRSMPN 431
           FA MY S+PN
Sbjct: 421 FAHMYHSVPN 429

[Close]

BLAST of CmaCh19G008520.1 vs. ExPASy TrEMBL
Match: A0A0A0K343 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G031700 PE=3 SV=1)

HSP 1 Score: 771.2 bits (1990), Expect = 2.4e-219
Identity = 361/435 (82.99%), Postives = 395/435 (90.80%), Query Frame = 0


Query: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFGPWFSDNIREDPYAGFG 60
           MAINQKPR KD+AL+RFKLV+ VTLLG LLFMIL ETNL  NF  W SD+I EDPY GFG
Sbjct: 1   MAINQKPRPKDAALKRFKLVVFVTLLGLLLFMILTETNLSWNFAAWSSDDIDEDPYLGFG 60

Query: 61  RFDSNDVLKLPKQTELSVRLEKRNRLPLRNLDLYPNLAKDHIPIVLYVHNRPQYLRVVID 120
             DSN  L+LPKQ++LS+RLEKRNRLP RNLDLYP LA +HI IVLYVHNRPQYLRVV+D
Sbjct: 61  --DSNVELRLPKQSQLSIRLEKRNRLPPRNLDLYPKLANNHIAIVLYVHNRPQYLRVVVD 120

Query: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPNSFPGVSPTDC 180
           SL+KV GISETLLIVSHDGYFEEMDKLVQ+IRFC VKQIFAPYSPHLFPNSFPGVSP DC
Sbjct: 121 SLAKVIGISETLLIVSHDGYFEEMDKLVQSIRFCQVKQIFAPYSPHLFPNSFPGVSPADC 180

Query: 181 KGKHYPMITTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240
           KGK  P    +CRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGL+ET+G+SGHILFIEE
Sbjct: 181 KGKDDPK-AVHCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKETQGMSGHILFIEE 240

Query: 241 DHFILPNAYRNLQLLVTLKPKKCPECHAVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300
           DHFILPNAYRNLQLLVTLKPKKCPEC++VNLAPSDVKSRGEG+NFL+AERMGNIGYAFNR
Sbjct: 241 DHFILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSRGEGSNFLIAERMGNIGYAFNR 300

Query: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD 360
           +VW+KI+K+AKEFCFFDEYNWDITMWATV+PSFG PVYSLRGPW SA+HFGKCGLHQG  
Sbjct: 301 TVWRKIYKQAKEFCFFDEYNWDITMWATVYPSFGKPVYSLRGPWASAVHFGKCGLHQGHG 360

Query: 361 EGDVCIDNGLLNIDVKDIDEVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420
           + D+CIDNG+LNIDVKDID+V NINSEW V++FR QPGYGAGFRGWGGWGDERDRQLCLN
Sbjct: 361 DNDICIDNGVLNIDVKDIDKVANINSEWRVDIFRDQPGYGAGFRGWGGWGDERDRQLCLN 420

Query: 421 FAQMYRSM---PNVS 433
           FA+MY SM   PN+S
Sbjct: 421 FARMYHSMSNGPNIS 432

[Close]

BLAST of CmaCh19G008520.1 vs. NCBI nr
Match: XP_022969101.1 (alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [Cucurbita maxima])

HSP 1 Score: 919.5 bits (2375), Expect = 1.1e-263
Identity = 434/434 (100.00%), Postives = 434/434 (100.00%), Query Frame = 0


Query: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFGPWFSDNIREDPYAGFG 60
           MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFGPWFSDNIREDPYAGFG
Sbjct: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFGPWFSDNIREDPYAGFG 60

Query: 61  RFDSNDVLKLPKQTELSVRLEKRNRLPLRNLDLYPNLAKDHIPIVLYVHNRPQYLRVVID 120
           RFDSNDVLKLPKQTELSVRLEKRNRLPLRNLDLYPNLAKDHIPIVLYVHNRPQYLRVVID
Sbjct: 61  RFDSNDVLKLPKQTELSVRLEKRNRLPLRNLDLYPNLAKDHIPIVLYVHNRPQYLRVVID 120

Query: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPNSFPGVSPTDC 180
           SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPNSFPGVSPTDC
Sbjct: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPNSFPGVSPTDC 180

Query: 181 KGKHYPMITTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240
           KGKHYPMITTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE
Sbjct: 181 KGKHYPMITTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240

Query: 241 DHFILPNAYRNLQLLVTLKPKKCPECHAVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300
           DHFILPNAYRNLQLLVTLKPKKCPECHAVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR
Sbjct: 241 DHFILPNAYRNLQLLVTLKPKKCPECHAVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300

Query: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD 360
           SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD
Sbjct: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD 360

Query: 361 EGDVCIDNGLLNIDVKDIDEVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420
           EGDVCIDNGLLNIDVKDIDEVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN
Sbjct: 361 EGDVCIDNGLLNIDVKDIDEVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420

Query: 421 FAQMYRSMPNVSRL 435
           FAQMYRSMPNVSRL
Sbjct: 421 FAQMYRSMPNVSRL 434

[Close]

BLAST of CmaCh19G008520.1 vs. NCBI nr
Match: KAG7011860.1 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 894.0 bits (2309), Expect = 5.0e-256
Identity = 420/433 (97.00%), Postives = 428/433 (98.85%), Query Frame = 0


Query: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFGPWFSDNIREDPYAGFG 60
           MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNF PW SDNIREDPYAGFG
Sbjct: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFAPWSSDNIREDPYAGFG 60

Query: 61  RFDSNDVLKLPKQTELSVRLEKRNRLPLRNLDLYPNLAKDHIPIVLYVHNRPQYLRVVID 120
           RFDSND LKLPKQTELS+RLEKRNRLPLRNLDLYPNLAKDHIPI+LYVHNRPQYLRVVID
Sbjct: 61  RFDSNDELKLPKQTELSIRLEKRNRLPLRNLDLYPNLAKDHIPILLYVHNRPQYLRVVID 120

Query: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPNSFPGVSPTDC 180
           SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFP+SFPGVSPTDC
Sbjct: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDC 180

Query: 181 KGKHYPMITTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240
            GKHYPM+TTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE
Sbjct: 181 IGKHYPMLTTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240

Query: 241 DHFILPNAYRNLQLLVTLKPKKCPECHAVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300
           DHFILPNAYRNLQLLV+LKPKKCPEC++VNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR
Sbjct: 241 DHFILPNAYRNLQLLVSLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300

Query: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD 360
           SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD
Sbjct: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD 360

Query: 361 EGDVCIDNGLLNIDVKDIDEVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420
           EGDVCIDNGLLNIDVKDID+VVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN
Sbjct: 361 EGDVCIDNGLLNIDVKDIDKVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420

Query: 421 FAQMYRSMPNVSR 434
           FAQMYRS PNVSR
Sbjct: 421 FAQMYRSTPNVSR 433

[Close]

BLAST of CmaCh19G008520.1 vs. NCBI nr
Match: XP_023554482.1 (alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 893.6 bits (2308), Expect = 6.5e-256
Identity = 418/433 (96.54%), Postives = 428/433 (98.85%), Query Frame = 0


Query: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFGPWFSDNIREDPYAGFG 60
           MA+NQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNF PW SDNIREDPYAGFG
Sbjct: 1   MAMNQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFAPWSSDNIREDPYAGFG 60

Query: 61  RFDSNDVLKLPKQTELSVRLEKRNRLPLRNLDLYPNLAKDHIPIVLYVHNRPQYLRVVID 120
           RFDSND LKLPKQTELS+RLEKRNRLPLRNLDLYPN+AKDHIPIVLYVHNRPQYLRVVID
Sbjct: 61  RFDSNDELKLPKQTELSIRLEKRNRLPLRNLDLYPNIAKDHIPIVLYVHNRPQYLRVVID 120

Query: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPNSFPGVSPTDC 180
           SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFP+SFPGVSPTDC
Sbjct: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDC 180

Query: 181 KGKHYPMITTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240
            GKHYPM+TTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE
Sbjct: 181 MGKHYPMLTTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240

Query: 241 DHFILPNAYRNLQLLVTLKPKKCPECHAVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300
           DHFILPNAYRNLQLLV+LKPKKCPEC++VNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR
Sbjct: 241 DHFILPNAYRNLQLLVSLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300

Query: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD 360
           SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD
Sbjct: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD 360

Query: 361 EGDVCIDNGLLNIDVKDIDEVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420
           EGDVCIDNGLLNIDVKDID+VVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN
Sbjct: 361 EGDVCIDNGLLNIDVKDIDKVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420

Query: 421 FAQMYRSMPNVSR 434
           FAQMYRS PN+SR
Sbjct: 421 FAQMYRSTPNISR 433

[Close]

BLAST of CmaCh19G008520.1 vs. NCBI nr
Match: KAG6572221.1 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 892.5 bits (2305), Expect = 1.5e-255
Identity = 419/433 (96.77%), Postives = 427/433 (98.61%), Query Frame = 0


Query: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFGPWFSDNIREDPYAGFG 60
           MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNF PW SDNIREDPYAGFG
Sbjct: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFAPWSSDNIREDPYAGFG 60

Query: 61  RFDSNDVLKLPKQTELSVRLEKRNRLPLRNLDLYPNLAKDHIPIVLYVHNRPQYLRVVID 120
           RFDSND LKLPKQTELS+RLEKRNRLPLRNLDLYPNLAKDHIPI+LYVHNRPQYLRVVID
Sbjct: 61  RFDSNDELKLPKQTELSIRLEKRNRLPLRNLDLYPNLAKDHIPILLYVHNRPQYLRVVID 120

Query: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPNSFPGVSPTDC 180
           SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFP+SFPGVSPTDC
Sbjct: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDC 180

Query: 181 KGKHYPMITTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240
            GKHYPM+TTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE
Sbjct: 181 IGKHYPMLTTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240

Query: 241 DHFILPNAYRNLQLLVTLKPKKCPECHAVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300
           DHFILPNAYRNLQLLV+LKPKKCPEC++VNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR
Sbjct: 241 DHFILPNAYRNLQLLVSLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300

Query: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD 360
           SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD
Sbjct: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD 360

Query: 361 EGDVCIDNGLLNIDVKDIDEVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420
           EGDVCIDNGLLNIDVKDID+VVNINSEWPVEVFRGQPGYG GFRGWGGWGDERDRQLCLN
Sbjct: 361 EGDVCIDNGLLNIDVKDIDKVVNINSEWPVEVFRGQPGYGVGFRGWGGWGDERDRQLCLN 420

Query: 421 FAQMYRSMPNVSR 434
           FAQMYRS PNVSR
Sbjct: 421 FAQMYRSTPNVSR 433

[Close]

BLAST of CmaCh19G008520.1 vs. NCBI nr
Match: XP_022952309.1 (alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [Cucurbita moschata])

HSP 1 Score: 891.3 bits (2302), Expect = 3.2e-255
Identity = 419/433 (96.77%), Postives = 426/433 (98.38%), Query Frame = 0


Query: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFGPWFSDNIREDPYAGFG 60
           MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNF PW SDNIREDPYAGFG
Sbjct: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFAPWSSDNIREDPYAGFG 60

Query: 61  RFDSNDVLKLPKQTELSVRLEKRNRLPLRNLDLYPNLAKDHIPIVLYVHNRPQYLRVVID 120
           RFDSND LKLPKQTELS+RLEKRNRLPLRNLDLYPNLAKDHIPIVLYVHNRPQYLRVVID
Sbjct: 61  RFDSNDELKLPKQTELSIRLEKRNRLPLRNLDLYPNLAKDHIPIVLYVHNRPQYLRVVID 120

Query: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPNSFPGVSPTDC 180
           SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFP+SFPGVSPTDC
Sbjct: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDC 180

Query: 181 KGKHYPMITTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240
            GKHYPM+TTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE
Sbjct: 181 MGKHYPMLTTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240

Query: 241 DHFILPNAYRNLQLLVTLKPKKCPECHAVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300
           DHFILPNAYRNLQ LV+LKPKKCPEC++VNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR
Sbjct: 241 DHFILPNAYRNLQFLVSLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300

Query: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD 360
           SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGS VYSLRGPWTSAIHFGKCGLHQGQD
Sbjct: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSSVYSLRGPWTSAIHFGKCGLHQGQD 360

Query: 361 EGDVCIDNGLLNIDVKDIDEVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420
           EGDVCIDNGLLNIDVKDID+VVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN
Sbjct: 361 EGDVCIDNGLLNIDVKDIDKVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420

Query: 421 FAQMYRSMPNVSR 434
           FAQMYRS PNVSR
Sbjct: 421 FAQMYRSTPNVSR 433

[Close]

BLAST of CmaCh19G008520.1 vs. TAIR 10
Match: AT2G05320.1 (beta-1,2-N-acetylglucosaminyltransferase II )

HSP 1 Score: 568.2 bits (1463), Expect = 5.8e-162
Identity = 272/423 (64.30%), Postives = 328/423 (77.54%), Query Frame = 0


Query: 5   QKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFGPWFSDNIREDPYAGFGRFDS 64
           +K RL+D+ L R  ++  VTL   L+ + +  T L  +      D + +D  A      +
Sbjct: 6   KKQRLRDTGLCRLGILFAVTLSIVLMLVSVPRTALNGSSIDDDLDGLDKDLEAKL----N 65

Query: 65  NDVLKLPKQTELSVRLEKRNRLPLRNLDLYPNLAKDHIPIVLYVHNRPQYLRVVIDSLSK 124
             +L + +   +S+RL +RN    RN DL+P+LAKD + IVLYVHNR QY RV ++SLSK
Sbjct: 66  ASLLSVARGNRMSLRLHRRNHFSPRNTDLFPDLAKDRVVIVLYVHNRAQYFRVTVESLSK 125

Query: 125 VTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPNSFPGVSPTDCKGKH 184
           V GISETLLIVSHDGYFEEM+++V++I+FC VKQIF+PYSPH++  SFPGV+  DCK K 
Sbjct: 126 VKGISETLLIVSHDGYFEEMNRIVESIKFCQVKQIFSPYSPHIYRTSFPGVTLNDCKNKG 185

Query: 185 YPMITTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEEDHFI 244
                 +C GNPDQYGNHR PKIVSLKHHWWWMMNTVWDGLEET+G  GHILFIEEDHF+
Sbjct: 186 -DEAKGHCEGNPDQYGNHRSPKIVSLKHHWWWMMNTVWDGLEETKGHEGHILFIEEDHFL 245

Query: 245 LPNAYRNLQLLVTLKPKKCPECHAVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNRSVWK 304
            PNAYRN+Q L  LKP KCP+C A NLAPSDVKSRGEG   LVAERMGN+GY+FNRSVW+
Sbjct: 246 FPNAYRNIQTLTRLKPAKCPDCFAANLAPSDVKSRGEGLESLVAERMGNVGYSFNRSVWE 305

Query: 305 KIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQ-DEGD 364
            IH+KA+EFCFFD+YNWDITMWATVFPSFGSPVY+LRGP TSA+HFGKCGLHQG+ DEGD
Sbjct: 306 NIHQKAREFCFFDDYNWDITMWATVFPSFGSPVYTLRGPRTSAVHFGKCGLHQGRGDEGD 365

Query: 365 VCIDNGLLNIDVKDIDEVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLNFAQ 424
            CIDNG++NI+VK+ D+VVNI   W V V++ Q GY AGF GWGGWGD+RDR LCL+FA 
Sbjct: 366 -CIDNGVVNIEVKETDKVVNIKEGWGVRVYKHQAGYKAGFEGWGGWGDDRDRHLCLDFAT 422

Query: 425 MYR 427
           MYR
Sbjct: 426 MYR 422

The following BLAST results are available for this feature:

BLAST of CmaCh19G008520.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FT88	8.2e-161	64.30	Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Arabid...	[more]
Q10469	4.5e-50	33.80	Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Homo s...	[more]
O19071	2.9e-49	32.21	Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Sus sc...	[more]
Q09326	8.5e-49	31.83	Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Rattus...	[more]
Q921V5	2.5e-48	31.74	Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Mus mu...	[more]

BLAST of CmaCh19G008520.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HWS5	5.4e-264	100.00	Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucurb...	[more]
A0A6J1GLB7	1.6e-255	96.77	Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucurb...	[more]
A0A5A7UTF0	4.2e-224	85.12	Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucumi...	[more]
A0A1S3CG23	4.2e-224	85.12	Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucumi...	[more]
A0A0A0K343	2.4e-219	82.99	Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucumi...	[more]

BLAST of CmaCh19G008520.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022969101.1	1.1e-263	100.00	alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [Cucurbit...	[more]
KAG7011860.1	5.0e-256	97.00	Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, partial ...	[more]
XP_023554482.1	6.5e-256	96.54	alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [Cucurbit...	[more]
KAG6572221.1	1.5e-255	96.77	Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, partial ...	[more]
XP_022952309.1	3.2e-255	96.77	alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [Cucurbit...	[more]

BLAST of CmaCh19G008520.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G05320.1	5.8e-162	64.30	beta-1,2-N-acetylglucosaminyltransferase II	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007754	N-acetylglucosaminyltransferase II	PFAM	PF05060	MGAT2	coord: 85..418 e-value: 3.8E-100 score: 335.3
IPR007754	N-acetylglucosaminyltransferase II	PANTHER	PTHR12871	BETA-1,2-N-ACETYLGLUCOSAMINYLTRANSFERASE II	coord: 6..425
IPR029044	Nucleotide-diphospho-sugar transferases	GENE3D	3.90.550.10	Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A	coord: 101..361 e-value: 1.7E-27 score: 98.4
IPR029044	Nucleotide-diphospho-sugar transferases	SUPERFAMILY	53448	Nucleotide-diphospho-sugar transferases	coord: 100..421
None	No IPR available	PANTHER	PTHR12871:SF0	ALPHA-1,6-MANNOSYL-GLYCOPROTEIN 2-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASE	coord: 6..425

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmaCh19G008520	CmaCh19G008520	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh19G008520.1:exon:2196	CmaCh19G008520.1:exon:2196	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh19G008520.1:cds	CmaCh19G008520.1:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmaCh19G008520.1	CmaCh19G008520.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009312	oligosaccharide biosynthetic process
biological_process	GO:0006487	protein N-linked glycosylation
cellular_component	GO:0005795	Golgi stack
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0000139	Golgi membrane
molecular_function	GO:0008455	alpha-1,6-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity
molecular_function	GO:0046872	metal ion binding