CmaCh13G004860.1 (mRNA) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh13G004860.1
TypemRNA
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description3-hydroxyisobutyrate dehydrogenase
LocationCma_Chr13: 5225216 .. 5233284 (-)
Sequence length1466
RNA-Seq ExpressionCmaCh13G004860.1
SyntenyCmaCh13G004860.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAGCCGAGGCCACTGTCGTAAATTTGCAGGAAGGGAAAGCCAATGCGAAGACGGATCAGTTCTTAATTATTGATCGAAAAGATTCTTTTATAGGCTTTTGATGTGTACGATTGAAGTGATTTTTTCAGTGATCATTTCTTCTTCTTCTTCCTCTTCCTTCCGTCGTCTGTGAACTGACGTGAAGGAGCCATCAATGGCGGTTTCTAGAGTCAGAGCTTCTTTCATGCAGTGCCTGAGGAAATCTCGTTTTAAACCTTTTGCGGTTGAGAGTGAGACATCTACTATACGTAGATTCTTCTCTGCTGATTCTTCGGCTTCATTCCAGGCAAGTTCTTTTCATCTTCGGAATCATTTGTTTATGGCATTGAGAAAATTGTTGCGGTAGCTTTAATTTTTTTTATTTTTTATTTTTATTTCTCCTTCTCTTTCTGAAATGTAATTTCTTGCCCGTAGAGCCAAATGATGATCGGTCTTTTATGGAATTCAACGAACTAGCTAATCTGCGGTTTGAACTGCTGGAAACATTTCAAATTTTAAGTGAATGCTTTGTTCTCGACGACGTGATTATCAAATTCTACTGTTTTTTTTTTTTCCAATTATCAGATTAATTGAAAACTTCAACCTGAAGTTTGTTATATCTATAATTGGATTAATTTTATAAGTATCTAAATTTAAGTCAAATTCAATCTTTAGTTCCTGTGATTGAAGCATAGTCTCACTCTCAACTTCTTATCGCATGATCTTATACTTTTTGAGTCGATAAAATGTGGCTTACTGCTATTTAATTCTTTGTTTCTGGCTCCTCAATTCAATAGTTAGGAAATGTGGATTTGATCTTCTTTCACGTTCTGTTCTGACATGTTTTCAGACTATAAAGAGTGTCTCCAATAATGTTTTGCAGAGTGTTGGATTCATTGGACTGGGAAATATGGGATCCAGAATGGCAAATAATATAATAAAGGCTGGATACAAAATGGTTGTACATGACATGTAATGTACTTAATCTTCTGATGCTACTAAAGGCCACTGTAAAGATTTTGATAAACTGTCCTTTTATTTCGGAAATTTGTTTTAATTTCTTTGCAAAAGCTCCAAAAACAAGTTCTCATGATTGGACGGAAGTGAATACAGTAGGAAACAAGCATGGATTTGGTGAATTGCATCTGATATATTTTTCTGTGGAAAACATTTTGCTACTTTTTGATTAAAAAATTGAGAGCGGTTTTACTCACATTAACTGTGGCATAATATTCTTTTCTATTAGAAACCGCAATACCATGGAGAAGTACTCAGATATGGGAGTTGCCACCAAAGAAACACCTTTTGAAGTTGCCGAAGCTTCTGACGTTGTCATTACAATGCTACCCTCTCCTTCTCATGTGAGTTGTGACCTATGTTTTCTCCCTTTCCTCCTGAAGGGGTCTAATCATGTTACCTGCAGTATCAATTTATTTATTTTATTAGGTTGTGAGTTGTGACTGCATGTTTTATGCTTTGACCTTATGCTATATTCTCCTTCATTCAAGTCCGAACATTATGTTCTATGATAGGTTTATTTAAGCACTTCTAGGTATGTACAGGATGATTATTATGAGATGTATAATATACATATGCAATTACCATGAGAGGGGAGGAAGAATTATATTATATTGATAGATGATTCATCATGTGGATGTTGGAGATGAAGTACTCTCTCTCTCTAAGTGAAATCTTGTCCCTCGTTGTCTCTCACCACTCTAATCTACCATTGATGGGAAATCATATTTGTAATGCAACTTTCTATAGACAAGGGATCGATAAAAATGGAAAAGAAGACGATGAAGTTCGAAAATAAAGTTCTCTCAATTTCTTTTGTCCAAGAAAAAAAGGGTAGGTACATCAAGATTATGGAATGACACATTAGACGCTCCTTTGAAGTTACAATCAAAGAAATTGTTGTTCTCTCAATTGTTGAGGCGCTGGAAGACTTGCTCTGTGCTCATCTCTCACAAAAGTTTTTTAGAAAATTGGAGTCAACGGAAGCCTCATTTGGATTCAAAGGACGACAAACAGAAGAGCCAGTTTCGTAGAGTTAACCAAAGTCTAGAGTTCAGGGGGCAAGAGAAACATTGGCATCCTTTATAACAAAGACTTCAAAGGTTGGGCAGTTTTCAAAACACTTTTGTGTGACTTTTTTTTTTTAACAAGAAGACAGACCACAAGATTTCTCTATTGGTAGGAAAAAGGCTCCATATATCTCTCAGAATGTGTCGTTTGCAGAGGCTTTGAAAAATGGTTAAAAAAAACATGGGAAAAATGAAAGTTTGAAGGTATGGGAGGAGGCTCATGGTTTCTCGACAAAAGAAGCAAGCAGAGAAGATGTGAGGCATCTTGAATGGGAAGAAACCATAGTGATTACACGAAGAGACTTTCACGTGGCTGGGGTAAGGTTCTTGAAATCCCTTTCAACCTGACAAAGCTCTCCTTAAATGTCCTTTGGTGGAGTGTGCTTGTATTCTTGTTACCAATAAAGGGTGGGTCACCTTTGGGCCTATGACAATTAAGTTAGAGCTTGGAGCCCCCTCATTCATGGTCGAACCAGTGTTGCCATTTCTTAAGGGAGTTGGGTTAAATTGAGAAACATCCCCCTGCACTTCTGGAGACTGGAAACATTTAAAGCTGTGGAGATGCGTTTGGAGGTTTTTTGGATTATACTGATTTGATTTATTGGATCATCGTTTGCTTTGAGTTTGCAGGAAAGGTTTAAGGTAACTAATGTGACTTTCTTCCTTCTGAAATTAAATTATTAGATAGGGGGACTTCTTTCTTGGCTTAGGTTTGTATTTTTGAAGATGGTAACTGGCTGATTGTAAAAGAAGTCGGAGCCCACAAAGGCTTTTTGCTAGAGGCGGCTAACATTTTTACAAGCAAGGAAATGGGTCCTCGACAAATCTAGTGGATATTTGGAGAGTTCATGATGGCATCAATTGCCTGGTGATCAAAATTTGGTACTCTGAGACCTTGGGATCTAATAGTCCAATTGGCACTGAAGGACAATTTCAATTTTGGGTGATTTCTTTATCGTCATTCAGATTGCTTGCTTTGATGGTTTTAGCGGTTAGATATCTGGCTTATATGGTCCTACACCGAAGGATAGAGATAAGTTTTGGCAAGGGATTTGTGATCTTTATGACCTGAGTATGAATCATTGGCGTATAGGAGAGAATTTTAATGTTGTCAGATGGATTCTGAAAATTTGAGAGGGTGCTGCATTACCTGAAGCATGTCACAGTTTAATACTCTTATTGAGGATTTGATTCTTGTAGATATTCCCATGAAAAATGGTGACTTCACTTAACCTACTATCAGAGGTGCTCTTTTAGCCATGCTTATTAGACAGATTTCTAGTCTCCTAGGGTTGGGTTGATCACTTCAGAGTGGTGGAGTTAGCTAGATTTGGGCAACAACAAATGGACCCTCCCCTTTTAGGTTTGAAAATGTTTGGTTAGATCATTTGCAGTTCTTAGCAAAAGTTGAGTTGTGGTGGAATTCTATGCTCTTCCAAGGTTGGTCGGGCTACATGTTTATAGAGAGGATAAAGGTTAAAGGGCTTCCTAAAAGTGTAATAAGGAGGAGTTTGGCAGAGTGTAAGATTCCAAACGTCATATCCAAGACAAAATAGGCGAGTTAGATAGCTTGAAAGATAGAGGCTTAATGAATTCGACTTTGAATGCCAAAGAAATAGTTTAAAGATATTATCAGTTCAATGATAGATATCATCCTTAGTATTCTTTTTATTCGCTTGGTGCCAGGTGGTCTATATATTAGCTACTTTATAAAACAATAAATATTCTAATTTGTGCATGTAGGTGCTTGAAGTGTATAATGGTTCACATGGTTTGCTTCATGGTGGCTATCATCTAAGGCCGTGGTTATTAATAGACTCCTCCACCATTGATCCACGAACATCAAGAGAGCTTTCAGCAATTGTATCCAGCCGTATTTTAAAGGAAAAGAGAGGTACCTGGTTTTGAATCAAAGAATTGAGGGCCTAATTCCTAACAACAATAATCGAGAGTTCTTATTTTTACTTTTAGATTAGGAGTTGGTTTCAAGTTGAGTAGGCAAGGCGAGGAAATAAATGGAAGAGAGGTTGCATATTTATGACAATCACTGACACCATGACTGATTTTCTATCTGATTTGTTTTCTTTTAATTCATAGTGTTCTGTTTTCTTAGTCCATAATATTATGTTCTTTTTATAATCATTTGTGCTTCTATTTTCTAATAATTCAACGAGCCAGCCATCATTAAACAAAAACCCAATCTCTCAGTCCGCAATTATGTGTATTCATTGTATCTCGTCACTGATTTTATTTGCAGATTCTTGGGAAAACCCTGTCATGTTGGATGCCCCTGTATCTGGAGGAGTTCTAGCTGCTGATGCTGGGACACTTACGTTCATGGTATGATTCATTTTAAGCCTCCGGTAACTTTGTATGTGTTGTATTTCGATGGAATTTTTTGCCACTTTGATTCAAAAGAAGCAGATGCTGCTTACGGAAATAGTATGGAATATTGTCATGTTTATCTAGTTTCATTAATTTACAAGTTCTTGAAGTGTGAAAGGAATGAACATAGAGACATTAGACAAATCCCCTGGACCTGCTGAAACTTGAATGATGTAGAACGTGGTAATCTTTTATGACGGCAATGCAATTGGCAAAGAAAAAATTGATAACACGATGCCTTGGCTAGCACTCAATGGTTCTGAATTTACAGTGTTTTGCTACTCTAGTTTTAATATGGGTCCACTCCTTTCTTAGTAATAAACCCTTTTTTCTTGTATTGTAATTGAAATATTCTGATTTGGGTGTTATATCATGACTGATAAAGTAGAGTAATGATCATGAATAATGGTTCACCGCGGGGAGAAGCTGTGAGAGGTTTTTGGTTTATTGCCCCTCTAAATAGGAGGCTTCTTGTGTCAGCCATCCAAGAGCGGATCGTGTGGTCAATTTTTCATTTATATAGAAAATTTGGTTAGTTCACAGGAAGTTTTTTGTTGACCTAAACATTTACTAGTTTGGGTGCAAGTGATCTTTATACTTTCGGGATTCTCAATATGAAAGTAATGCCACCTCTTCTTTTATGATAACTAACTGAAGAGTTGTTATTGCAAATTTTTACCAGTAGATTGGTCGAATACTAGTCTACCCCTCCTGTTCGTGGATGTGTGCATTTATTGTTTGTGCTGCTAGCTCTTCTTCTTACTTCTATTTTAAGAAACCAGCTTCTATTACAATTATATGACATAGCAGTTCTGACCAGGGGGGTGGGACATCACACCAGAACTTGAGACTGGTTTAATAGCCTTTTTTATGAAGCAGGAGGGAAATTCTAAGTGTTATTACAATAAGTAGCTTTATTTTTTCAATAACAGAAATGGAAGCAACTGCTTAAAAGAGAACAATTGTTTCAATGGCCATAAAATTTTTGTGGCTTTTACAATTTGGATCTTATTTCTAATTGGATTCTAGTCTCTAGGTTTGTCTCGTCTTCGTTATTTTTCTTTATCAATTTCGTATTCTTTTTTCAACCTACGTCAATTTCTGACTACAAGATATCATATCCTCCTCAGGTTGGTGGTCCTGAGGAATCTTACAATGCTGCAAAAACATTGTTCCTTTCAATGGGCAAAAGTTCAGTTTATTGTGGTGGAGCTGGAAATGGTTCGGTAAGTTTCATTTCTTCTTTTGTTATAAGTTTGTGATCTCTGGGGATTTAAAAGCGGTGTGAGTTCTATAAATGATTGTAGCTACAGCGTTTGGTCTTGATGACATACTAAATATAGGTATTTTGCAGTTGCTTAATAAGTGAGAAAACTAGTTTTGAGCACTTGGTCTCACATCATGATTCATGATTACTTGATTATTGAATAAAATGTTATAGGTGGCTTGTTTGCTTTGCAAGCGTTTATTGCCTTAAATACAAAACAAATGCTTCTAGTTTAACGTGATAACAAAAATGATTCTTCCATGGTATAAAAATGTACATTTATACATATATAGTTTGGAAAAATCAACAAAATGGCCATTATTTGTCAAAGTTCCACCAGTAGATGCCATGTTCTCTCTATTTAAGGGAGACTTGATCAGCCTAAAGATGTTCTCTTTTGTTGTAATGCTCATTTATTTTTTGAAAATCGAAGTTTGTTAGCTATGAAACTTCAAACCACCAACTAAAATGGTATAACTTGCAGTGACTTTCTTTATCCTTAAACATCTCGATCATTTACAGACTTTTTACCTAATTTTCCATCAAGTATGATTTCAACTTTTGTAACATATGGTATTAATGTCATTCTCAGAGGATCTGTTGCCCGACTCTGATACTTGTAATATTGTTCTATCATAGGCAGCAAAGATTTGCAACAACTTAGCAATGGCTGTGAGTATGCTTGGTGTTTCAGAAGCTTTGGCTCTAGGCCAGTCTCTTGGGATTTCTGCAAAAACTCTAACAAAAGTATTAAATTCATCAAGTGCTCGCTGTTGGAGTAGGTATGTATGTTACTCTAATGAGATCATAGTAATATTTCCATCCCCGCAGGTTCAACTTAAAAAATTCTTATTTGTTTCATGCTGGCCCAGTTGCAGCAAGTTTGCTGACATGCCTGGAATATACAATTAATGTTGTTAGTTTGATTCATATAGTTTTTTTAATCATCAGTCTAGGCATTGGATGATTGAAGTTTTTCTGTGAAATTTTGAACTGAAATATATCTTAAATTTAAAGTTCCTGGTTTAGTAGCGTTTTGGAGGAATAAAAATATCAAATTGTTATTTCTTTCCTCATGGTAAACCTTCTCTATTCATGAAAGCTGATCAATGCTGAGTAATTTGAGGTTGGTTTAATCGTCAAGGATTGAAGTCCGTTCCTTTAGTCAGTGCTTGGACTCCCTTTTTTGTGCCCTTATACATTTTAAAACTCGGTTTCTTACTGCAAATTAATGTTGGATAATTGGATACAAAGTGAAAGTTACAATCCAGTTCCTGGGGTGATGGAGAAGGTGCCCTCTGCGAGTGATTATGATGGTGGATTTGCATCAAAGCTTATGGTTAGTCATTCAAACCTGTTCATTCTTCAAAGGAAAAGTATATGTCATTATATCCCTGGAATCGTATGCTTCAGTGCTTTTTAATAAGTTTATGGCTCGAAGGATGCTTCATTTTTATGTTGAAAACCATTTCCAATCTGTTTTCTTGAACAAGGAGAAAATAGACTGGCCCCCACACCGTTGGAGACCTTTCTGATTAATTATGCTGAACATCAGTCTTCACTAATTAAGCTGCTCTCTTATATGGAACTATGATGGCTTGGATCGATGAGAAAATACTCTTTTTTCTGGAGCAAGGGTTTTTGTTCCTCAAAAAAAGATAAATTACAAGTCTATGTACTCTTAAATTTAAGAGAGGTTTGCCAGTTTTGTGCTTGTGCTTTTATAATGGTAGTGCTTTTCAATGAAATATTATTTAAAGAGCTCATATCGTCAAGGGCTTCATTAGGGTGTATTTTAGTGTTTTTAAACTCTTCAAAGATCACTTTTTTACTACAATTAGATACTGACAAAATTTTAAAATTTATATATTTATTTAGCTAGAGCTTTCTTCACTGGTCTTAAAATCTTGCTCTGATTTCTTGTTCAACGCAGGCTAAAGATCTAAACCTTGCAGCAGCATCAGCTAGTGAAATTGGCCTCGAGCATCCACTGACGTCCCAGGCGCAAGAGATGTAAGTTTAGTGGGGAAAAACTTATCTTATTCTCTCCTTTTTAGTGCACATTGCGGAACCACCTTTAACTACAAAAATTATTACACTATGAACTTTTAATTGTATAAATTAAACCCTCAAACTTATAACTTACACCTCTATTTATAAAAATTAAACGTTCCAACTTTCTAAATGAAAATATTGAAACCCTTCGCCTCCCCCTCCCTTGGTAACCCTTTATTTTTTTTAAATGCTCCCATGTGAATGTTATTACATTTAAACTCTTCATTTGTCAGATACAAAAAGCTATGCGAGGATG

mRNA sequence

GAAGCCGAGGCCACTGTCGTAAATTTGCAGGAAGGGAAAGCCAATGCGAAGACGGATCAGTTCTTAATTATTGATCGAAAAGATTCTTTTATAGGCTTTTGATGTGTACGATTGAAGTGATTTTTTCAGTGATCATTTCTTCTTCTTCTTCCTCTTCCTTCCGTCGTCTGTGAACTGACGTGAAGGAGCCATCAATGGCGGTTTCTAGAGTCAGAGCTTCTTTCATGCAGTGCCTGAGGAAATCTCGTTTTAAACCTTTTGCGGTTGAGAGTGAGACATCTACTATACGTAGATTCTTCTCTGCTGATTCTTCGGCTTCATTCCAGAGTGTTGGATTCATTGGACTGGGAAATATGGGATCCAGAATGGCAAATAATATAATAAAGGCTGGATACAAAATGGTTGTACATGACATAAACCGCAATACCATGGAGAAGTACTCAGATATGGGAGTTGCCACCAAAGAAACACCTTTTGAAGTTGCCGAAGCTTCTGACGTTGTCATTACAATGCTACCCTCTCCTTCTCATGTGCTTGAAGTGTATAATGGTTCACATGGTTTGCTTCATGGTGGCTATCATCTAAGGCCGTGGTTATTAATAGACTCCTCCACCATTGATCCACGAACATCAAGAGAGCTTTCAGCAATTGTATCCAGCCGTATTTTAAAGGAAAAGAGAGATTCTTGGGAAAACCCTGTCATGTTGGATGCCCCTGTATCTGGAGGAGTTCTAGCTGCTGATGCTGGGACACTTACGTTCATGGTTGGTGGTCCTGAGGAATCTTACAATGCTGCAAAAACATTGTTCCTTTCAATGGGCAAAAGTTCAGTTTATTGTGGTGGAGCTGGAAATGGTTCGGCAGCAAAGATTTGCAACAACTTAGCAATGGCTGTGAGTATGCTTGGTGTTTCAGAAGCTTTGGCTCTAGGCCAGTCTCTTGGGATTTCTGCAAAAACTCTAACAAAAGTATTAAATTCATCAAGTGCTCGCTGTTGGAGTAGTGAAAGTTACAATCCAGTTCCTGGGGTGATGGAGAAGGTGCCCTCTGCGAGTGATTATGATGGTGGATTTGCATCAAAGCTTATGGCTAAAGATCTAAACCTTGCAGCAGCATCAGCTAGTGAAATTGGCCTCGAGCATCCACTGACGTCCCAGGCGCAAGAGATGTAAGTTTAGTGGGGAAAAACTTATCTTATTCTCTCCTTTTTAGTGCACATTGCGGAACCACCTTTAACTACAAAAATTATTACACTATGAACTTTTAATTGTATAAATTAAACCCTCAAACTTATAACTTACACCTCTATTTATAAAAATTAAACGTTCCAACTTTCTAAATGAAAATATTGAAACCCTTCGCCTCCCCCTCCCTTGGTAACCCTTTATTTTTTTTAAATGCTCCCATGTGAATGTTATTACATTTAAACTCTTCATTTGTCAGATACAAAAAGCTATGCGAGGATG

Coding sequence (CDS)

ATGGCGGTTTCTAGAGTCAGAGCTTCTTTCATGCAGTGCCTGAGGAAATCTCGTTTTAAACCTTTTGCGGTTGAGAGTGAGACATCTACTATACGTAGATTCTTCTCTGCTGATTCTTCGGCTTCATTCCAGAGTGTTGGATTCATTGGACTGGGAAATATGGGATCCAGAATGGCAAATAATATAATAAAGGCTGGATACAAAATGGTTGTACATGACATAAACCGCAATACCATGGAGAAGTACTCAGATATGGGAGTTGCCACCAAAGAAACACCTTTTGAAGTTGCCGAAGCTTCTGACGTTGTCATTACAATGCTACCCTCTCCTTCTCATGTGCTTGAAGTGTATAATGGTTCACATGGTTTGCTTCATGGTGGCTATCATCTAAGGCCGTGGTTATTAATAGACTCCTCCACCATTGATCCACGAACATCAAGAGAGCTTTCAGCAATTGTATCCAGCCGTATTTTAAAGGAAAAGAGAGATTCTTGGGAAAACCCTGTCATGTTGGATGCCCCTGTATCTGGAGGAGTTCTAGCTGCTGATGCTGGGACACTTACGTTCATGGTTGGTGGTCCTGAGGAATCTTACAATGCTGCAAAAACATTGTTCCTTTCAATGGGCAAAAGTTCAGTTTATTGTGGTGGAGCTGGAAATGGTTCGGCAGCAAAGATTTGCAACAACTTAGCAATGGCTGTGAGTATGCTTGGTGTTTCAGAAGCTTTGGCTCTAGGCCAGTCTCTTGGGATTTCTGCAAAAACTCTAACAAAAGTATTAAATTCATCAAGTGCTCGCTGTTGGAGTAGTGAAAGTTACAATCCAGTTCCTGGGGTGATGGAGAAGGTGCCCTCTGCGAGTGATTATGATGGTGGATTTGCATCAAAGCTTATGGCTAAAGATCTAAACCTTGCAGCAGCATCAGCTAGTGAAATTGGCCTCGAGCATCCACTGACGTCCCAGGCGCAAGAGATGTAA

Protein sequence

MAVSRVRASFMQCLRKSRFKPFAVESETSTIRRFFSADSSASFQSVGFIGLGNMGSRMANNIIKAGYKMVVHDINRNTMEKYSDMGVATKETPFEVAEASDVVITMLPSPSHVLEVYNGSHGLLHGGYHLRPWLLIDSSTIDPRTSRELSAIVSSRILKEKRDSWENPVMLDAPVSGGVLAADAGTLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVSEALALGQSLGISAKTLTKVLNSSSARCWSSESYNPVPGVMEKVPSASDYDGGFASKLMAKDLNLAAASASEIGLEHPLTSQAQEM
Homology
BLAST of CmaCh13G004860.1 vs. ExPASy Swiss-Prot
Match: Q9SUC0 (Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g20930 PE=1 SV=3)

HSP 1 Score: 415.2 bits (1066), Expect = 6.7e-115
Identity = 215/320 (67.19%), Postives = 267/320 (83.44%), Query Frame = 0

Query: 7   RASFMQCLRKSRFKPFAVESETSTIRRF-FSADSSASFQSVGFIGLGNMGSRMANNIIKA 66
           RA  + CL K     F     + ++ RF  S+ +S  FQ+VGFIGLGNMG RM NN+I+A
Sbjct: 5   RAQTLLCLSK-----FKTNFVSGSLHRFSSSSQNSNQFQNVGFIGLGNMGFRMVNNLIRA 64

Query: 67  GYKMVVHDINRNTMEKYSDMGVATKETPFEVAEASDVVITMLPSPSHVLEVYNGSHGLLH 126
           GYK+ VHDINR+ M+ +++MGV+++ETP+EVA+ S+VVITMLPS SHV++VY G++GLL 
Sbjct: 65  GYKVTVHDINRDVMKMFTEMGVSSRETPYEVAQDSEVVITMLPSSSHVMDVYTGTNGLLL 124

Query: 127 GGYHLRPWLLIDSSTIDPRTSRELSAIVSSRILKEKRDSWENPVMLDAPVSGGVLAADAG 186
           G   +RP L IDSSTIDP+T+R++S  VS+  LKEKRD+WE PVMLDAPVSGGVLAA+AG
Sbjct: 125 GENDIRPALFIDSSTIDPQTTRKISLAVSNCNLKEKRDNWEKPVMLDAPVSGGVLAAEAG 184

Query: 187 TLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVSEALAL 246
           TLTFMVGGPE++Y AA+ +  SMG++S+YCGG+GNGSAAKICNNLAMAVSMLG SEALAL
Sbjct: 185 TLTFMVGGPEDAYLAARPILQSMGRTSIYCGGSGNGSAAKICNNLAMAVSMLGTSEALAL 244

Query: 247 GQSLGISAKTLTKVLNSSSARCWSSESYNPVPGVMEKVPSASDYDGGFASKLMAKDLNLA 306
           GQSLGISA TLT+VLN+SS RCWSS++YNPVPGVM+ VPS+ DY+GGFASKLMAKDLNLA
Sbjct: 245 GQSLGISASTLTEVLNTSSGRCWSSDAYNPVPGVMKGVPSSRDYNGGFASKLMAKDLNLA 304

Query: 307 AASASEIGLEHPLTSQAQEM 326
           AASA E+G + PL S+AQE+
Sbjct: 305 AASAEEVGHKSPLISKAQEI 319

BLAST of CmaCh13G004860.1 vs. ExPASy Swiss-Prot
Match: Q2HJD7 (3-hydroxyisobutyrate dehydrogenase, mitochondrial OS=Bos taurus OX=9913 GN=HIBADH PE=2 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 6.8e-67
Identity = 149/309 (48.22%), Postives = 199/309 (64.40%), Query Frame = 0

Query: 17  SRFKPFAVESETSTIRRFFSADSSASFQSVGFIGLGNMGSRMANNIIKAGYKMVVHDINR 76
           SR +P AV S  +   R     S AS   VGFIG+GNMG+ MA N++K GY ++++D+  
Sbjct: 18  SRRQPPAVASLAAVCSR-----SMASKTPVGFIGVGNMGNPMAKNLMKHGYPLIIYDVFP 77

Query: 77  NTMEKYSDMGVATKETPFEVAEASDVVITMLPSPSHVLEVYNGSHGLLHGGYHLRPWLLI 136
           +  +++ D G     +P +VAE +D +ITMLP+  + +E Y+G++G+L      +  LLI
Sbjct: 78  DACKEFLDAGEQVVSSPADVAEKADRIITMLPTSINAIEAYSGANGILK--KVKKGSLLI 137

Query: 137 DSSTIDPRTSRELSAIVSSRILKEKRDSWENPVMLDAPVSGGVLAADAGTLTFMVGGPEE 196
           DSSTIDP  S+EL+  V               V +DAPVSGGV AA +G LTFMVGG EE
Sbjct: 138 DSSTIDPMVSKELAKEVEKM----------GAVFMDAPVSGGVGAARSGNLTFMVGGVEE 197

Query: 197 SYNAAKTLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVSEALALGQSLGISAKTL 256
            + AA+ L   MG + VYCG  G G AAKICNNL +A+SM+G +EA+ LG  LG+  K L
Sbjct: 198 EFAAAQELLGCMGSNVVYCGAVGTGQAAKICNNLLLAISMIGTAEAMNLGIRLGLDPKLL 257

Query: 257 TKVLNSSSARCWSSESYNPVPGVMEKVPSASDYDGGFASKLMAKDLNLAAASASEIGLEH 316
            K+LN SS RCWSS++YNPVPGVM+ VPSA++Y GGF + LMAKDL LA  SA+      
Sbjct: 258 AKILNMSSGRCWSSDTYNPVPGVMDGVPSANNYQGGFGTTLMAKDLGLAQDSATSTKSPI 309

Query: 317 PLTSQAQEM 326
            L SQA ++
Sbjct: 318 LLGSQAHQI 309

BLAST of CmaCh13G004860.1 vs. ExPASy Swiss-Prot
Match: Q54CX6 (Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=hibA PE=3 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 7.5e-66
Identity = 136/288 (47.22%), Postives = 185/288 (64.24%), Query Frame = 0

Query: 39  SSASFQSVGFIGLGNMGSRMANNIIKAGYKMVVHDINRNTMEKYSDMGVATKETPFEVAE 98
           S++S ++VGFIGLGNMG   A N+IK G+ ++V D++++ M +  + G     +P EVA+
Sbjct: 18  STSSSKTVGFIGLGNMGGHQAINLIKKGHNLIVFDMSKDNMNRLKEKGAKIANSPAEVAK 77

Query: 99  ASDVVITMLPSPSHVLEVYNGSHGLLHGGYHLRP-WLLIDSSTIDPRTSRELSAIVSSRI 158
            +DV++TMLP+ +HV  VY G +G+      +RP  LL+DSSTIDP T+RE+++I     
Sbjct: 78  EADVIVTMLPASAHVKNVYCGENGIFQ---TVRPGTLLLDSSTIDPATAREVASIAKK-- 137

Query: 159 LKEKRDSWENPVMLDAPVSGGVLAADAGTLTFMVGGPEESYNAAKTLFLSMGKSSVYCGG 218
                       MLD PVSGG   A+AGTLTFMVGG E+ +N AKT    MGK+ V+CG 
Sbjct: 138 --------HQSTMLDCPVSGGTGGAEAGTLTFMVGGSEQDFNTAKTYLECMGKNIVHCGD 197

Query: 219 AGNGSAAKICNNLAMAVSMLGVSEALALGQSLGISAKTLTKVLNSSSARCWSSESYNPVP 278
            G G  AK+CNNL + +SM+ VSEA+ LG   G+  K L  + N+SSARCW+SE YNP P
Sbjct: 198 VGTGQVAKVCNNLVLGISMIAVSEAMNLGVKQGMDPKKLAGIFNTSSARCWTSELYNPCP 257

Query: 279 GVMEKVPSASDYDGGFASKLMAKDLNLAAASASEIGLEHPLTSQAQEM 326
           GV+E  P++  Y GGF S LM KDL LA  SA  IG    L + A ++
Sbjct: 258 GVIETSPASRGYTGGFGSALMTKDLGLAVDSAKSIGEPLLLGNSAHQL 292

BLAST of CmaCh13G004860.1 vs. ExPASy Swiss-Prot
Match: P31937 (3-hydroxyisobutyrate dehydrogenase, mitochondrial OS=Homo sapiens OX=9606 GN=HIBADH PE=1 SV=2)

HSP 1 Score: 251.5 bits (641), Expect = 1.3e-65
Identity = 137/272 (50.37%), Postives = 184/272 (67.65%), Query Frame = 0

Query: 39  SSASFQSVGFIGLGNMGSRMANNIIKAGYKMVVHDINRNTMEKYSDMGVATKETPFEVAE 98
           S AS   VGFIGLGNMG+ MA N++K GY ++++D+  +  +++ D G     +P +VAE
Sbjct: 35  SVASKTPVGFIGLGNMGNPMAKNLMKHGYPLIIYDVFPDACKEFQDAGEQVVSSPADVAE 94

Query: 99  ASDVVITMLPSPSHVLEVYNGSHGLLHGGYHLRPWLLIDSSTIDPRTSRELSAIVSSRIL 158
            +D +ITMLP+  + +E Y+G++G+L      +  LLIDSSTIDP  S+EL+  V     
Sbjct: 95  KADRIITMLPTSINAIEAYSGANGILK--KVKKGSLLIDSSTIDPAVSKELAKEVEKM-- 154

Query: 159 KEKRDSWENPVMLDAPVSGGVLAADAGTLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGA 218
                     V +DAPVSGGV AA +G LTFMVGG E+ + AA+ L   MG + VYCG  
Sbjct: 155 --------GAVFMDAPVSGGVGAARSGNLTFMVGGVEDEFAAAQELLGCMGSNVVYCGAV 214

Query: 219 GNGSAAKICNNLAMAVSMLGVSEALALGQSLGISAKTLTKVLNSSSARCWSSESYNPVPG 278
           G G AAKICNN+ +A+SM+G +EA+ LG  LG+  K L K+LN SS RCWSS++YNPVPG
Sbjct: 215 GTGQAAKICNNMLLAISMIGTAEAMNLGIRLGLDPKLLAKILNMSSGRCWSSDTYNPVPG 274

Query: 279 VMEKVPSASDYDGGFASKLMAKDLNLAAASAS 311
           VM+ VPSA++Y GGF + LMAKDL LA  SA+
Sbjct: 275 VMDGVPSANNYQGGFGTTLMAKDLGLAQDSAT 294

BLAST of CmaCh13G004860.1 vs. ExPASy Swiss-Prot
Match: Q5R5E7 (3-hydroxyisobutyrate dehydrogenase, mitochondrial OS=Pongo abelii OX=9601 GN=HIBADH PE=2 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 1.3e-65
Identity = 137/272 (50.37%), Postives = 184/272 (67.65%), Query Frame = 0

Query: 39  SSASFQSVGFIGLGNMGSRMANNIIKAGYKMVVHDINRNTMEKYSDMGVATKETPFEVAE 98
           S AS   VGFIGLGNMG+ MA N++K GY ++++D+  +  +++ D G     +P +VAE
Sbjct: 35  SVASKTPVGFIGLGNMGNPMAKNLMKHGYPLIIYDVFPDACKEFQDAGEQVVSSPADVAE 94

Query: 99  ASDVVITMLPSPSHVLEVYNGSHGLLHGGYHLRPWLLIDSSTIDPRTSRELSAIVSSRIL 158
            +D +ITMLP+  + +E Y+G++G+L      +  LLIDSSTIDP  S+EL+  V     
Sbjct: 95  KADRIITMLPTSINAIEAYSGANGILK--KVKKGSLLIDSSTIDPAVSKELAKEVEKM-- 154

Query: 159 KEKRDSWENPVMLDAPVSGGVLAADAGTLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGA 218
                     V +DAPVSGGV AA +G LTFMVGG E+ + AA+ L   MG + VYCG  
Sbjct: 155 --------GAVFMDAPVSGGVGAARSGNLTFMVGGVEDEFAAAQELLGCMGSNVVYCGAV 214

Query: 219 GNGSAAKICNNLAMAVSMLGVSEALALGQSLGISAKTLTKVLNSSSARCWSSESYNPVPG 278
           G G AAKICNN+ +A+SM+G +EA+ LG  LG+  K L K+LN SS RCWSS++YNPVPG
Sbjct: 215 GTGQAAKICNNMLLAISMIGTAEAMNLGIRLGLDPKLLAKILNMSSGRCWSSDTYNPVPG 274

Query: 279 VMEKVPSASDYDGGFASKLMAKDLNLAAASAS 311
           VM+ VPSA++Y GGF + LMAKDL LA  SA+
Sbjct: 275 VMDGVPSANNYQGGFGATLMAKDLGLAQDSAT 294

BLAST of CmaCh13G004860.1 vs. ExPASy TrEMBL
Match: A0A6J1KJE2 (3-hydroxyisobutyrate dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111495162 PE=3 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 6.0e-175
Identity = 324/325 (99.69%), Postives = 325/325 (100.00%), Query Frame = 0

Query: 1   MAVSRVRASFMQCLRKSRFKPFAVESETSTIRRFFSADSSASFQSVGFIGLGNMGSRMAN 60
           MAVSRVRASFMQCLRKSRFKPFAVESETSTIRRFFSADSSASFQSVGFIGLGNMGSRMAN
Sbjct: 1   MAVSRVRASFMQCLRKSRFKPFAVESETSTIRRFFSADSSASFQSVGFIGLGNMGSRMAN 60

Query: 61  NIIKAGYKMVVHDINRNTMEKYSDMGVATKETPFEVAEASDVVITMLPSPSHVLEVYNGS 120
           NIIKAGYKMVVHDINRNTMEKYSDMGVATKETPFEVAEASDVVITMLPSPSHVLEVYNGS
Sbjct: 61  NIIKAGYKMVVHDINRNTMEKYSDMGVATKETPFEVAEASDVVITMLPSPSHVLEVYNGS 120

Query: 121 HGLLHGGYHLRPWLLIDSSTIDPRTSRELSAIVSSRILKEKRDSWENPVMLDAPVSGGVL 180
           HGLLHGGYHLRPWLLIDSSTIDPRTSRELSAIVSSRILKEKRDSWENPVMLDAPVSGGVL
Sbjct: 121 HGLLHGGYHLRPWLLIDSSTIDPRTSRELSAIVSSRILKEKRDSWENPVMLDAPVSGGVL 180

Query: 181 AADAGTLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS 240
           AADAGTLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS
Sbjct: 181 AADAGTLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS 240

Query: 241 EALALGQSLGISAKTLTKVLNSSSARCWSSESYNPVPGVMEKVPSASDYDGGFASKLMAK 300
           EALALGQSLGISAKTLTKVLNSSSARCWSSESYNPVPGVMEKVPSASDYDGGFASKLMAK
Sbjct: 241 EALALGQSLGISAKTLTKVLNSSSARCWSSESYNPVPGVMEKVPSASDYDGGFASKLMAK 300

Query: 301 DLNLAAASASEIGLEHPLTSQAQEM 326
           DLNLAAASASEIGLEHPLTSQAQE+
Sbjct: 301 DLNLAAASASEIGLEHPLTSQAQEI 325

BLAST of CmaCh13G004860.1 vs. ExPASy TrEMBL
Match: A0A6J1EHZ4 (3-hydroxyisobutyrate dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111434338 PE=3 SV=1)

HSP 1 Score: 616.3 bits (1588), Expect = 7.3e-173
Identity = 322/326 (98.77%), Postives = 325/326 (99.69%), Query Frame = 0

Query: 1   MAVSRVRASFMQCLRK-SRFKPFAVESETSTIRRFFSADSSASFQSVGFIGLGNMGSRMA 60
           MAVSRVRASFMQCLRK SRFKPFAVESETS+IRRFFSADSSASFQSVGFIGLGNMGSRMA
Sbjct: 1   MAVSRVRASFMQCLRKSSRFKPFAVESETSSIRRFFSADSSASFQSVGFIGLGNMGSRMA 60

Query: 61  NNIIKAGYKMVVHDINRNTMEKYSDMGVATKETPFEVAEASDVVITMLPSPSHVLEVYNG 120
           NN+IKAGYKMVVHDINRNTMEKYSDMGVATKETPFEVAEASDVVITMLPSPSHVLEVYNG
Sbjct: 61  NNLIKAGYKMVVHDINRNTMEKYSDMGVATKETPFEVAEASDVVITMLPSPSHVLEVYNG 120

Query: 121 SHGLLHGGYHLRPWLLIDSSTIDPRTSRELSAIVSSRILKEKRDSWENPVMLDAPVSGGV 180
           SHGLLHGGYHLRPWLLIDSSTIDPRTSRELSAIVSSRILKEKRDSWENPVMLDAPVSGGV
Sbjct: 121 SHGLLHGGYHLRPWLLIDSSTIDPRTSRELSAIVSSRILKEKRDSWENPVMLDAPVSGGV 180

Query: 181 LAADAGTLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGV 240
           LAADAGTLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGV
Sbjct: 181 LAADAGTLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGV 240

Query: 241 SEALALGQSLGISAKTLTKVLNSSSARCWSSESYNPVPGVMEKVPSASDYDGGFASKLMA 300
           SEALALGQSLGISAKTLTKVLNSSSARCWSSESYNPVPGVMEKVPSASDYDGGFASKLMA
Sbjct: 241 SEALALGQSLGISAKTLTKVLNSSSARCWSSESYNPVPGVMEKVPSASDYDGGFASKLMA 300

Query: 301 KDLNLAAASASEIGLEHPLTSQAQEM 326
           KDLNLAAASASEIGLEHPLTSQAQE+
Sbjct: 301 KDLNLAAASASEIGLEHPLTSQAQEI 326

BLAST of CmaCh13G004860.1 vs. ExPASy TrEMBL
Match: A0A0A0M152 (3-hydroxyisobutyrate dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_1G538190 PE=3 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 4.3e-149
Identity = 276/325 (84.92%), Postives = 299/325 (92.00%), Query Frame = 0

Query: 1   MAVSRVRASFMQCLRKSRFKPFAVESETSTIRRFFSADSSASFQSVGFIGLGNMGSRMAN 60
           MA+ R  ASF   + KS FK F V +ET +IRRFFSADS ASFQS+GFIGLGNMG+RMAN
Sbjct: 1   MAIFRAAASFRHYMSKSCFKSFPVGNETPSIRRFFSADSLASFQSIGFIGLGNMGTRMAN 60

Query: 61  NIIKAGYKMVVHDINRNTMEKYSDMGVATKETPFEVAEASDVVITMLPSPSHVLEVYNGS 120
           N+IKAGY MVVHDINRNTM+ YSD+GVATKETPFEVAEASDVVITMLPSPSHVL+VYNGS
Sbjct: 61  NLIKAGYNMVVHDINRNTMKNYSDLGVATKETPFEVAEASDVVITMLPSPSHVLDVYNGS 120

Query: 121 HGLLHGGYHLRPWLLIDSSTIDPRTSRELSAIVSSRILKEKRDSWENPVMLDAPVSGGVL 180
           HGLLHGG H+RPWLLIDSSTIDP+TSRELSA+VSSRILKEKR SWE+PVMLDAPVSGGVL
Sbjct: 121 HGLLHGGCHIRPWLLIDSSTIDPQTSRELSAVVSSRILKEKRGSWESPVMLDAPVSGGVL 180

Query: 181 AADAGTLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS 240
           AADA TLTFMVGG EE+Y+AAK LFLSMGK SVYCGG+GNGSAAKICNNLA+AVSMLGVS
Sbjct: 181 AADARTLTFMVGGLEEAYHAAKALFLSMGKGSVYCGGSGNGSAAKICNNLALAVSMLGVS 240

Query: 241 EALALGQSLGISAKTLTKVLNSSSARCWSSESYNPVPGVMEKVPSASDYDGGFASKLMAK 300
           EALALGQSLGISAKTLTKV NSSSARCWSSESYNPVPGVME+VPSA DYDGGFA+KLM K
Sbjct: 241 EALALGQSLGISAKTLTKVFNSSSARCWSSESYNPVPGVMEEVPSARDYDGGFATKLMFK 300

Query: 301 DLNLAAASASEIGLEHPLTSQAQEM 326
           DLNLA ASA E+GL+HPLTSQAQE+
Sbjct: 301 DLNLAKASAGEVGLQHPLTSQAQEI 325

BLAST of CmaCh13G004860.1 vs. ExPASy TrEMBL
Match: A0A1S3BBY5 (3-hydroxyisobutyrate dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103488390 PE=3 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 9.6e-149
Identity = 276/325 (84.92%), Postives = 298/325 (91.69%), Query Frame = 0

Query: 1   MAVSRVRASFMQCLRKSRFKPFAVESETSTIRRFFSADSSASFQSVGFIGLGNMGSRMAN 60
           MA+ R  ASF Q + KSRFK F V SET +IRRFFSAD+ ASFQSVGFIGLGNMG+RMAN
Sbjct: 1   MAIFRATASFRQYMSKSRFKSFPVGSETPSIRRFFSADALASFQSVGFIGLGNMGTRMAN 60

Query: 61  NIIKAGYKMVVHDINRNTMEKYSDMGVATKETPFEVAEASDVVITMLPSPSHVLEVYNGS 120
           N+IKAGY MVVHDINRNTM+ YSD GVATKETPFEVAEASDVVITMLPSPSHVL+VYNGS
Sbjct: 61  NLIKAGYNMVVHDINRNTMKNYSDRGVATKETPFEVAEASDVVITMLPSPSHVLDVYNGS 120

Query: 121 HGLLHGGYHLRPWLLIDSSTIDPRTSRELSAIVSSRILKEKRDSWENPVMLDAPVSGGVL 180
           HGLLHGG H+RPWLLIDSSTIDP+TSRELSA+VSS +LKEKR SWE+PVMLDAPVSGGVL
Sbjct: 121 HGLLHGGSHIRPWLLIDSSTIDPQTSRELSAVVSSCVLKEKRGSWESPVMLDAPVSGGVL 180

Query: 181 AADAGTLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS 240
           AADA TLTFMVGG EE+Y+AAK LFL+MGK SVYCGGAGNGSAAKICNNLA+AVSMLGVS
Sbjct: 181 AADARTLTFMVGGLEEAYHAAKALFLAMGKGSVYCGGAGNGSAAKICNNLALAVSMLGVS 240

Query: 241 EALALGQSLGISAKTLTKVLNSSSARCWSSESYNPVPGVMEKVPSASDYDGGFASKLMAK 300
           EALALGQSLGISAKTLTKV NSSSARCWSSESYNPVPGVME+VPSA DYDGGFA+KLM K
Sbjct: 241 EALALGQSLGISAKTLTKVFNSSSARCWSSESYNPVPGVMEEVPSARDYDGGFATKLMVK 300

Query: 301 DLNLAAASASEIGLEHPLTSQAQEM 326
           DLNLA ASASE+GL+HPLTSQ  E+
Sbjct: 301 DLNLAKASASEVGLQHPLTSQGLEI 325

BLAST of CmaCh13G004860.1 vs. ExPASy TrEMBL
Match: A0A6J1CHH7 (3-hydroxyisobutyrate dehydrogenase OS=Momordica charantia OX=3673 GN=LOC111010940 PE=3 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 2.8e-148
Identity = 278/325 (85.54%), Postives = 297/325 (91.38%), Query Frame = 0

Query: 1   MAVSRVRASFMQCLRKSRFKPFAVESETSTIRRFFSADSSASFQSVGFIGLGNMGSRMAN 60
           MAVSRVRASF Q + KS FK F  E+ T ++RRFFSAD+ ASFQSVGFIGLGNMGSRMAN
Sbjct: 1   MAVSRVRASFAQYMIKSNFKHFPGETATPSVRRFFSADALASFQSVGFIGLGNMGSRMAN 60

Query: 61  NIIKAGYKMVVHDINRNTMEKYSDMGVATKETPFEVAEASDVVITMLPSPSHVLEVYNGS 120
           N+IKAGYKMVVHDINR+TM+KYSDMGV+TKETPFEVAEASD+VITMLPS SHVL VY GS
Sbjct: 61  NLIKAGYKMVVHDINRDTMKKYSDMGVSTKETPFEVAEASDIVITMLPSSSHVLNVYTGS 120

Query: 121 HGLLHGGYHLRPWLLIDSSTIDPRTSRELSAIVSSRILKEKRDSWENPVMLDAPVSGGVL 180
           HGLLHGG HLRPWLLIDSSTIDPRTSRELSA+VS   L+EKRDSW NP MLDAPVSGGVL
Sbjct: 121 HGLLHGGSHLRPWLLIDSSTIDPRTSRELSAVVSGCSLREKRDSWGNPAMLDAPVSGGVL 180

Query: 181 AADAGTLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS 240
           AADAG LTFMVGG EE+Y++AK+LFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS
Sbjct: 181 AADAGKLTFMVGGSEEAYHSAKSLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS 240

Query: 241 EALALGQSLGISAKTLTKVLNSSSARCWSSESYNPVPGVMEKVPSASDYDGGFASKLMAK 300
           EALALGQ LGISA TLTKV NSSSARCWSSESYNPVPGVME+VPSA +YDGGFASKLMAK
Sbjct: 241 EALALGQCLGISANTLTKVFNSSSARCWSSESYNPVPGVMEEVPSARNYDGGFASKLMAK 300

Query: 301 DLNLAAASASEIGLEHPLTSQAQEM 326
           DLNLAAASA EIGL HPLTS AQE+
Sbjct: 301 DLNLAAASAVEIGLRHPLTSMAQEI 325

BLAST of CmaCh13G004860.1 vs. NCBI nr
Match: XP_023000830.1 (probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial isoform X1 [Cucurbita maxima])

HSP 1 Score: 623.2 bits (1606), Expect = 1.2e-174
Identity = 324/325 (99.69%), Postives = 325/325 (100.00%), Query Frame = 0

Query: 1   MAVSRVRASFMQCLRKSRFKPFAVESETSTIRRFFSADSSASFQSVGFIGLGNMGSRMAN 60
           MAVSRVRASFMQCLRKSRFKPFAVESETSTIRRFFSADSSASFQSVGFIGLGNMGSRMAN
Sbjct: 1   MAVSRVRASFMQCLRKSRFKPFAVESETSTIRRFFSADSSASFQSVGFIGLGNMGSRMAN 60

Query: 61  NIIKAGYKMVVHDINRNTMEKYSDMGVATKETPFEVAEASDVVITMLPSPSHVLEVYNGS 120
           NIIKAGYKMVVHDINRNTMEKYSDMGVATKETPFEVAEASDVVITMLPSPSHVLEVYNGS
Sbjct: 61  NIIKAGYKMVVHDINRNTMEKYSDMGVATKETPFEVAEASDVVITMLPSPSHVLEVYNGS 120

Query: 121 HGLLHGGYHLRPWLLIDSSTIDPRTSRELSAIVSSRILKEKRDSWENPVMLDAPVSGGVL 180
           HGLLHGGYHLRPWLLIDSSTIDPRTSRELSAIVSSRILKEKRDSWENPVMLDAPVSGGVL
Sbjct: 121 HGLLHGGYHLRPWLLIDSSTIDPRTSRELSAIVSSRILKEKRDSWENPVMLDAPVSGGVL 180

Query: 181 AADAGTLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS 240
           AADAGTLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS
Sbjct: 181 AADAGTLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS 240

Query: 241 EALALGQSLGISAKTLTKVLNSSSARCWSSESYNPVPGVMEKVPSASDYDGGFASKLMAK 300
           EALALGQSLGISAKTLTKVLNSSSARCWSSESYNPVPGVMEKVPSASDYDGGFASKLMAK
Sbjct: 241 EALALGQSLGISAKTLTKVLNSSSARCWSSESYNPVPGVMEKVPSASDYDGGFASKLMAK 300

Query: 301 DLNLAAASASEIGLEHPLTSQAQEM 326
           DLNLAAASASEIGLEHPLTSQAQE+
Sbjct: 301 DLNLAAASASEIGLEHPLTSQAQEI 325

BLAST of CmaCh13G004860.1 vs. NCBI nr
Match: KAG6583846.1 (putative 3-hydroxyisobutyrate dehydrogenase, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 619.4 bits (1596), Expect = 1.8e-173
Identity = 320/325 (98.46%), Postives = 325/325 (100.00%), Query Frame = 0

Query: 1   MAVSRVRASFMQCLRKSRFKPFAVESETSTIRRFFSADSSASFQSVGFIGLGNMGSRMAN 60
           MAVSR+RASFMQCLRKSRFKPFAVESETS+IRRFFSADSSASFQSVGFIGLGNMGSRMAN
Sbjct: 1   MAVSRIRASFMQCLRKSRFKPFAVESETSSIRRFFSADSSASFQSVGFIGLGNMGSRMAN 60

Query: 61  NIIKAGYKMVVHDINRNTMEKYSDMGVATKETPFEVAEASDVVITMLPSPSHVLEVYNGS 120
           N+IKAGYKMVVHDINRNTMEKYSDMGVATKETPFEVAEASDVVITMLPSPSHVLEVYNGS
Sbjct: 61  NLIKAGYKMVVHDINRNTMEKYSDMGVATKETPFEVAEASDVVITMLPSPSHVLEVYNGS 120

Query: 121 HGLLHGGYHLRPWLLIDSSTIDPRTSRELSAIVSSRILKEKRDSWENPVMLDAPVSGGVL 180
           HGLLHGGYHLRPWLLIDSSTIDPRTSRELSAIVSSRILKEKRDSWENPVMLDAPVSGGVL
Sbjct: 121 HGLLHGGYHLRPWLLIDSSTIDPRTSRELSAIVSSRILKEKRDSWENPVMLDAPVSGGVL 180

Query: 181 AADAGTLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS 240
           AADAGTLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS
Sbjct: 181 AADAGTLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS 240

Query: 241 EALALGQSLGISAKTLTKVLNSSSARCWSSESYNPVPGVMEKVPSASDYDGGFASKLMAK 300
           EALALGQSLGISAKTLTKVLNSSSARCWSSESYNPVPGVMEKVPSASDYDGGFASKLMAK
Sbjct: 241 EALALGQSLGISAKTLTKVLNSSSARCWSSESYNPVPGVMEKVPSASDYDGGFASKLMAK 300

Query: 301 DLNLAAASASEIGLEHPLTSQAQEM 326
           DLNLAAASA+EIGLEHPLTSQAQE+
Sbjct: 301 DLNLAAASATEIGLEHPLTSQAQEI 325

BLAST of CmaCh13G004860.1 vs. NCBI nr
Match: XP_022927541.1 (probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial isoform X1 [Cucurbita moschata])

HSP 1 Score: 616.3 bits (1588), Expect = 1.5e-172
Identity = 322/326 (98.77%), Postives = 325/326 (99.69%), Query Frame = 0

Query: 1   MAVSRVRASFMQCLRK-SRFKPFAVESETSTIRRFFSADSSASFQSVGFIGLGNMGSRMA 60
           MAVSRVRASFMQCLRK SRFKPFAVESETS+IRRFFSADSSASFQSVGFIGLGNMGSRMA
Sbjct: 1   MAVSRVRASFMQCLRKSSRFKPFAVESETSSIRRFFSADSSASFQSVGFIGLGNMGSRMA 60

Query: 61  NNIIKAGYKMVVHDINRNTMEKYSDMGVATKETPFEVAEASDVVITMLPSPSHVLEVYNG 120
           NN+IKAGYKMVVHDINRNTMEKYSDMGVATKETPFEVAEASDVVITMLPSPSHVLEVYNG
Sbjct: 61  NNLIKAGYKMVVHDINRNTMEKYSDMGVATKETPFEVAEASDVVITMLPSPSHVLEVYNG 120

Query: 121 SHGLLHGGYHLRPWLLIDSSTIDPRTSRELSAIVSSRILKEKRDSWENPVMLDAPVSGGV 180
           SHGLLHGGYHLRPWLLIDSSTIDPRTSRELSAIVSSRILKEKRDSWENPVMLDAPVSGGV
Sbjct: 121 SHGLLHGGYHLRPWLLIDSSTIDPRTSRELSAIVSSRILKEKRDSWENPVMLDAPVSGGV 180

Query: 181 LAADAGTLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGV 240
           LAADAGTLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGV
Sbjct: 181 LAADAGTLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGV 240

Query: 241 SEALALGQSLGISAKTLTKVLNSSSARCWSSESYNPVPGVMEKVPSASDYDGGFASKLMA 300
           SEALALGQSLGISAKTLTKVLNSSSARCWSSESYNPVPGVMEKVPSASDYDGGFASKLMA
Sbjct: 241 SEALALGQSLGISAKTLTKVLNSSSARCWSSESYNPVPGVMEKVPSASDYDGGFASKLMA 300

Query: 301 KDLNLAAASASEIGLEHPLTSQAQEM 326
           KDLNLAAASASEIGLEHPLTSQAQE+
Sbjct: 301 KDLNLAAASASEIGLEHPLTSQAQEI 326

BLAST of CmaCh13G004860.1 vs. NCBI nr
Match: XP_023520240.1 (probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 607.8 bits (1566), Expect = 5.3e-170
Identity = 317/325 (97.54%), Postives = 320/325 (98.46%), Query Frame = 0

Query: 1   MAVSRVRASFMQCLRKSRFKPFAVESETSTIRRFFSADSSASFQSVGFIGLGNMGSRMAN 60
           MAVSRV  SFMQ LRKS  KPFAVESETS+IRRFFSADSSASFQSVGFIGLGNMGSRMAN
Sbjct: 1   MAVSRVGTSFMQYLRKSHVKPFAVESETSSIRRFFSADSSASFQSVGFIGLGNMGSRMAN 60

Query: 61  NIIKAGYKMVVHDINRNTMEKYSDMGVATKETPFEVAEASDVVITMLPSPSHVLEVYNGS 120
           N+IKAGYKMVVHDINRNTMEKYSDMGVATKETPFEVAEASDVVITMLPSPSHVLEVYNGS
Sbjct: 61  NLIKAGYKMVVHDINRNTMEKYSDMGVATKETPFEVAEASDVVITMLPSPSHVLEVYNGS 120

Query: 121 HGLLHGGYHLRPWLLIDSSTIDPRTSRELSAIVSSRILKEKRDSWENPVMLDAPVSGGVL 180
           HGLLHGGYHLRPWLLIDSSTIDPRTSRELSAIVSSRILKEKRDSWENPVMLDAPVSGGVL
Sbjct: 121 HGLLHGGYHLRPWLLIDSSTIDPRTSRELSAIVSSRILKEKRDSWENPVMLDAPVSGGVL 180

Query: 181 AADAGTLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS 240
           AADAGTLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS
Sbjct: 181 AADAGTLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS 240

Query: 241 EALALGQSLGISAKTLTKVLNSSSARCWSSESYNPVPGVMEKVPSASDYDGGFASKLMAK 300
           EALALGQSLGISAKTLTKVLNSSSARCWSSESYNPVPGVMEKVPSASDYDGGFASKLMAK
Sbjct: 241 EALALGQSLGISAKTLTKVLNSSSARCWSSESYNPVPGVMEKVPSASDYDGGFASKLMAK 300

Query: 301 DLNLAAASASEIGLEHPLTSQAQEM 326
           DLNLAAASASEIGLEHPLTSQAQE+
Sbjct: 301 DLNLAAASASEIGLEHPLTSQAQEI 325

BLAST of CmaCh13G004860.1 vs. NCBI nr
Match: XP_038894392.1 (probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial isoform X2 [Benincasa hispida])

HSP 1 Score: 554.3 bits (1427), Expect = 7.0e-154
Identity = 289/326 (88.65%), Postives = 305/326 (93.56%), Query Frame = 0

Query: 1   MAVSRVRASFMQCLRKSRFKPFAVESET-STIRRFFSADSSASFQSVGFIGLGNMGSRMA 60
           MA+SR  ASFMQ   KS FK F V S+T S+ RRFFSAD+ ASFQSVGFIGLGNMG+RMA
Sbjct: 1   MALSRATASFMQYTSKSHFKSFWVGSQTPSSSRRFFSADAFASFQSVGFIGLGNMGTRMA 60

Query: 61  NNIIKAGYKMVVHDINRNTMEKYSDMGVATKETPFEVAEASDVVITMLPSPSHVLEVYNG 120
           NN+IKAGYKMVVHDIN N M+KYSDMGVATKETPFEVAEASDVVITMLPSPSHV EVYNG
Sbjct: 61  NNLIKAGYKMVVHDINCNIMKKYSDMGVATKETPFEVAEASDVVITMLPSPSHVREVYNG 120

Query: 121 SHGLLHGGYHLRPWLLIDSSTIDPRTSRELSAIVSSRILKEKRDSWENPVMLDAPVSGGV 180
           SHGLLHGGYHLRPWLLIDSSTIDP+TSRELSA VSSRILKEKRDSWENPVMLDAPVSGG+
Sbjct: 121 SHGLLHGGYHLRPWLLIDSSTIDPQTSRELSASVSSRILKEKRDSWENPVMLDAPVSGGI 180

Query: 181 LAADAGTLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGV 240
           LAADAGTLTFMVGGPEE+Y AAK+LFLSMGK SVYCGGAGNGSAAKICNNLA+AVSMLGV
Sbjct: 181 LAADAGTLTFMVGGPEEAYYAAKSLFLSMGKGSVYCGGAGNGSAAKICNNLALAVSMLGV 240

Query: 241 SEALALGQSLGISAKTLTKVLNSSSARCWSSESYNPVPGVMEKVPSASDYDGGFASKLMA 300
           SEALALGQSLGISAKTLTKV NSSSARCWSSESYNPVPGVME+VPSA DYDGGFASKLMA
Sbjct: 241 SEALALGQSLGISAKTLTKVFNSSSARCWSSESYNPVPGVMEEVPSARDYDGGFASKLMA 300

Query: 301 KDLNLAAASASEIGLEHPLTSQAQEM 326
           KDLNLAAASA E+GL+HPLTSQAQE+
Sbjct: 301 KDLNLAAASAGEVGLQHPLTSQAQEI 326

BLAST of CmaCh13G004860.1 vs. TAIR 10
Match: AT4G20930.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 415.2 bits (1066), Expect = 4.7e-116
Identity = 215/320 (67.19%), Postives = 267/320 (83.44%), Query Frame = 0

Query: 7   RASFMQCLRKSRFKPFAVESETSTIRRF-FSADSSASFQSVGFIGLGNMGSRMANNIIKA 66
           RA  + CL K     F     + ++ RF  S+ +S  FQ+VGFIGLGNMG RM NN+I+A
Sbjct: 5   RAQTLLCLSK-----FKTNFVSGSLHRFSSSSQNSNQFQNVGFIGLGNMGFRMVNNLIRA 64

Query: 67  GYKMVVHDINRNTMEKYSDMGVATKETPFEVAEASDVVITMLPSPSHVLEVYNGSHGLLH 126
           GYK+ VHDINR+ M+ +++MGV+++ETP+EVA+ S+VVITMLPS SHV++VY G++GLL 
Sbjct: 65  GYKVTVHDINRDVMKMFTEMGVSSRETPYEVAQDSEVVITMLPSSSHVMDVYTGTNGLLL 124

Query: 127 GGYHLRPWLLIDSSTIDPRTSRELSAIVSSRILKEKRDSWENPVMLDAPVSGGVLAADAG 186
           G   +RP L IDSSTIDP+T+R++S  VS+  LKEKRD+WE PVMLDAPVSGGVLAA+AG
Sbjct: 125 GENDIRPALFIDSSTIDPQTTRKISLAVSNCNLKEKRDNWEKPVMLDAPVSGGVLAAEAG 184

Query: 187 TLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVSEALAL 246
           TLTFMVGGPE++Y AA+ +  SMG++S+YCGG+GNGSAAKICNNLAMAVSMLG SEALAL
Sbjct: 185 TLTFMVGGPEDAYLAARPILQSMGRTSIYCGGSGNGSAAKICNNLAMAVSMLGTSEALAL 244

Query: 247 GQSLGISAKTLTKVLNSSSARCWSSESYNPVPGVMEKVPSASDYDGGFASKLMAKDLNLA 306
           GQSLGISA TLT+VLN+SS RCWSS++YNPVPGVM+ VPS+ DY+GGFASKLMAKDLNLA
Sbjct: 245 GQSLGISASTLTEVLNTSSGRCWSSDAYNPVPGVMKGVPSSRDYNGGFASKLMAKDLNLA 304

Query: 307 AASASEIGLEHPLTSQAQEM 326
           AASA E+G + PL S+AQE+
Sbjct: 305 AASAEEVGHKSPLISKAQEI 319

BLAST of CmaCh13G004860.1 vs. TAIR 10
Match: AT4G29120.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 118.2 bits (295), Expect = 1.2e-26
Identity = 89/285 (31.23%), Postives = 138/285 (48.42%), Query Frame = 0

Query: 46  VGFIGLGNMGSRMANNIIKAGYKMVVHDINRNTMEKYSDMGVATKETPFEVAEASDVVIT 105
           +G+IG G MG  M  ++IKAGY + V +   +  +   DMG    ++P  VAE SDVV T
Sbjct: 40  IGWIGTGVMGRSMCGHLIKAGYTVTVFNRTISKAQTLIDMGANVADSPNSVAEQSDVVFT 99

Query: 106 MLPSPSHVLEVY-----NGSHGLLHGGYHLRPWLLIDSSTIDPRTSRELSAIVSSRILKE 165
           ++  PS V  V          GL  GG      +L+D +T +P  + E++          
Sbjct: 100 IVGYPSDVRHVLLDPKSGALSGLRQGG------VLVDMTTSEPSLAEEIA---------- 159

Query: 166 KRDSWENPVMLDAPVSGGVLAADAGTLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGAGN 225
           K  S++N   +DAPVSGG L A  G L+   GG E +      LF  MGK + + G +G 
Sbjct: 160 KAASFKNCFSIDAPVSGGDLGAKNGKLSIFAGGDETTVKRLDPLFSLMGKVN-FMGTSGK 219

Query: 226 GSAAKICNNLAMAVSMLGVSEALALGQSLGISAKTLTKVLNSSSARCWSSESYNPVPGVM 285
           G  AK+ N + +A +MLG+ E L      G+  K   + +++ +A   S + Y     ++
Sbjct: 220 GQFAKLANQITIASTMLGLVEGLIYAHKAGLDVKKFLEAISTGAAGSKSIDLYG--DRIL 279

Query: 286 EKVPSASDYDGGFASKLMAKDLNLAAASASEIGLEHPLTSQAQEM 326
           ++     D+D GF      KDL +       +GL  P  + AQ++
Sbjct: 280 KR-----DFDPGFYVNHFVKDLGICLNECQRMGLALPGLALAQQL 300

BLAST of CmaCh13G004860.1 vs. TAIR 10
Match: AT1G17650.1 (glyoxylate reductase 2 )

HSP 1 Score: 117.9 bits (294), Expect = 1.6e-26
Identity = 89/288 (30.90%), Postives = 130/288 (45.14%), Query Frame = 0

Query: 45  SVGFIGLGNMGSRMANNIIKAGYKMVVHDINRNTMEKYSDMGVATKETPFEVAEASDVVI 104
           S+GF+G+G MGS MA N+IKAG  + V +  ++  +    +G   K +P EV    D+  
Sbjct: 68  SIGFLGMGIMGSPMAQNLIKAGCDVTVWNRTKSKCDPLVGLGAKYKSSPEEVTATCDLTF 127

Query: 105 TMLPSPSHVLEVYNGSHGLLHG-----GYHLRPWLLIDSSTIDPRTSRELSAIVSSRILK 164
            ML  P   ++V  G +G + G     GY       +D ST+D          V+S IL 
Sbjct: 128 AMLADPESAIDVACGKNGAIFGISSGKGY-------VDVSTVD----------VASSILI 187

Query: 165 EKRDSWENPVMLDAPVSGGVLAADAGTLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGAG 224
            K+      + L+APVSG    A+ G L F+  G +  Y  A      MGKS  Y G  G
Sbjct: 188 SKQIKDTGALFLEAPVSGSKKPAEDGQLIFLTAGDKPLYEKAAPFLDIMGKSKFYLGEVG 247

Query: 225 NGSAAKICNNLAMAVSMLGVSEALALGQSLGISAKTLTKVLNSSSARCWSSESYNPVPGV 284
           NG+A K+  N+ M   M   +E + L Q +G+    L +V++  +            P  
Sbjct: 248 NGAAMKLVVNMIMGSMMASFAEGILLSQKVGLDPNVLVEVVSQGAIN---------APMY 307

Query: 285 MEKVPS--ASDYDGGFASKLMAKDLNLAAASASEIGLEHPLTSQAQEM 326
             K PS   S Y   F  K   KD+ LA   A  +    P+ + A E+
Sbjct: 308 SLKGPSMIKSVYPTAFPLKHQQKDMRLALGLAESVSQSTPIAAAANEL 329

BLAST of CmaCh13G004860.1 vs. TAIR 10
Match: AT1G71170.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 101.3 bits (251), Expect = 1.5e-21
Identity = 77/265 (29.06%), Postives = 130/265 (49.06%), Query Frame = 0

Query: 46  VGFIGLGNMGSRMANNIIKAGYKMVVHDINRNTMEKYSDMGVATKETPFEVAEASDVVIT 105
           +G+IG+G MGS M ++I+ AGY + V+  +    +     G  T  +P E+ E SDVV T
Sbjct: 16  IGWIGIGIMGSAMVSHILAAGYSVTVYARDLRKTKDLQTKGGRTANSPKELGEMSDVVFT 75

Query: 106 MLPSPSHVLEVYNGSHGLLHGGYHLRP-WLLIDSSTIDPRTSRELSAIVSSRILKEKRDS 165
           ++ + + V  +  G  G+L G   L+P  + +D ++  P  +RE+ A         +RD 
Sbjct: 76  IVGNSNDVRSLLLGDDGVLSG---LKPGGVTVDMTSSKPGLAREIYAEA------RRRDC 135

Query: 166 WENPVMLDAPVSGGVLAADAGTLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGAGNGSAA 225
           W     +DAPVSGG   A  G LT   GG  E       +  +MG    + GGAG+G + 
Sbjct: 136 W----AVDAPVSGGDAGAREGKLTIFAGGDSEIVEWLAPVMKTMGIVR-FMGGAGSGQSC 195

Query: 226 KICNNLAMAVSMLGVSEALALGQSLGISAKTLTKVLNSSSARCWSSESYNPVPGVMEKVP 285
           KI N + +  +M+G++E +   +  G+      + +   +A        + V  +  ++ 
Sbjct: 196 KIGNQICVGSNMIGLAEGIVFAEKAGLDPVKWLEAVKDGAAG-------SAVMRLFGEMM 255

Query: 286 SASDYDGGFASKLMAKDLNLAAASA 310
           +  DY     ++ M KDL +AA +A
Sbjct: 256 AVRDYKATGFAEYMVKDLGMAAEAA 259

BLAST of CmaCh13G004860.1 vs. TAIR 10
Match: AT1G18270.1 (ketose-bisphosphate aldolase class-II family protein )

HSP 1 Score: 100.1 bits (248), Expect = 3.4e-21
Identity = 83/301 (27.57%), Postives = 142/301 (47.18%), Query Frame = 0

Query: 11  MQCLRKSRFKPFAVESETSTIRRFFSADSSASFQSVGFIGLGNMGSRMANNIIKAGYKMV 70
           ++   +  +KP  +  E +T         +     +GFIGLG MG  MA +++K+ + + 
Sbjct: 297 LEAANRELYKPEDLAKEITT--------QAKPVNRIGFIGLGAMGFGMAAHLLKSNFSVC 356

Query: 71  VHDINRNTMEKYSDMGVATKETPFEVAEASDVVITMLPSPSHVLEVYNGSHGLLHGGYHL 130
            +D+ + T+ ++ + G     +P EV +  DV++ M+ +     +V  G  G +      
Sbjct: 357 GYDVYKPTLVRFENAGGLAANSPAEVTKDVDVLVIMVTNEVQAEDVLYGHLGAVEAIPSG 416

Query: 131 RPWLLIDSSTIDPRTSRELSAIVSSRILKEKRDSWENPVMLDAPVSGGVLAADAGTLTFM 190
              +L  +ST+ P    +L      R+  E +D      ++DAPVSGGV  A  G LT M
Sbjct: 417 ATVVL--ASTVSPAFVSQL----ERRLENEGKDL----KLVDAPVSGGVKRAAMGELTIM 476

Query: 191 VGGPEESYNAAKTLFLSMG-KSSVYCGGAGNGSAAKICNNLAMAVSMLGVSEALALGQSL 250
             G +E+  +A  +  ++  K  V  GG G GS  K+ N L   V +   +EA+A G  L
Sbjct: 477 ASGTDEALKSAGLVLSALSEKLYVIKGGCGAGSGVKMVNQLLAGVHIASAAEAMAFGARL 536

Query: 251 GISAKTLTKVLNSSSARCWSSESYNPVPGVMEKVPSASDYDGGFASKLMAKDLNLAAASA 310
           G++ + L  V+++S    W  E  N VP +++     +DY    A  +  KDL +     
Sbjct: 537 GLNTRKLFNVISNSGGTSWMFE--NRVPHMLD-----NDYTPYSALDIFVKDLGIVTREG 572

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SUC06.7e-11567.19Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial OS=Arabidopsis thalia... [more]
Q2HJD76.8e-6748.223-hydroxyisobutyrate dehydrogenase, mitochondrial OS=Bos taurus OX=9913 GN=HIBAD... [more]
Q54CX67.5e-6647.22Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial OS=Dictyostelium disc... [more]
P319371.3e-6550.373-hydroxyisobutyrate dehydrogenase, mitochondrial OS=Homo sapiens OX=9606 GN=HIB... [more]
Q5R5E71.3e-6550.373-hydroxyisobutyrate dehydrogenase, mitochondrial OS=Pongo abelii OX=9601 GN=HIB... [more]
Match NameE-valueIdentityDescription
A0A6J1KJE26.0e-17599.693-hydroxyisobutyrate dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111495162 P... [more]
A0A6J1EHZ47.3e-17398.773-hydroxyisobutyrate dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111434338... [more]
A0A0A0M1524.3e-14984.923-hydroxyisobutyrate dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_1G538190 PE... [more]
A0A1S3BBY59.6e-14984.923-hydroxyisobutyrate dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103488390 PE=3 ... [more]
A0A6J1CHH72.8e-14885.543-hydroxyisobutyrate dehydrogenase OS=Momordica charantia OX=3673 GN=LOC11101094... [more]
Match NameE-valueIdentityDescription
XP_023000830.11.2e-17499.69probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial isoform X1 [Cucurbita... [more]
KAG6583846.11.8e-17398.46putative 3-hydroxyisobutyrate dehydrogenase, mitochondrial, partial [Cucurbita a... [more]
XP_022927541.11.5e-17298.77probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial isoform X1 [Cucurbita... [more]
XP_023520240.15.3e-17097.54probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial isoform X1 [Cucurbita... [more]
XP_038894392.17.0e-15488.65probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial isoform X2 [Benincasa... [more]
Match NameE-valueIdentityDescription
AT4G20930.14.7e-11667.196-phosphogluconate dehydrogenase family protein [more]
AT4G29120.11.2e-2631.236-phosphogluconate dehydrogenase family protein [more]
AT1G17650.11.6e-2630.90glyoxylate reductase 2 [more]
AT1G71170.11.5e-2129.066-phosphogluconate dehydrogenase family protein [more]
AT1G18270.13.4e-2127.57ketose-bisphosphate aldolase class-II family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0158153-hydroxyisobutyrate dehydrogenase-relatedPIRSFPIRSF000103HIBADHcoord: 39..325
e-value: 2.0E-64
score: 215.4
IPR0291543-hydroxyisobutyrate dehydrogenase, NAD-binding domainPFAMPF14833NAD_binding_11coord: 219..325
e-value: 5.7E-27
score: 94.4
NoneNo IPR availableGENE3D3.40.50.720coord: 39..217
e-value: 1.2E-51
score: 176.8
NoneNo IPR availablePIRSRPIRSR000103-1PIRSR000103-1coord: 45..325
e-value: 1.4E-74
score: 248.4
NoneNo IPR availablePANTHERPTHR229813-HYDROXYISOBUTYRATE DEHYDROGENASE-RELATEDcoord: 35..325
NoneNo IPR availablePANTHERPTHR22981:SF73-HYDROXYISOBUTYRATE DEHYDROGENASE, MITOCHONDRIALcoord: 35..325
IPR0115483-hydroxyisobutyrate dehydrogenaseTIGRFAMTIGR01692TIGR01692coord: 48..325
e-value: 1.1E-94
score: 314.9
IPR0061156-phosphogluconate dehydrogenase, NADP-bindingPFAMPF03446NAD_binding_2coord: 46..216
e-value: 1.5E-37
score: 129.2
IPR0133286-phosphogluconate dehydrogenase, domain 2GENE3D1.10.1040.10coord: 218..325
e-value: 1.2E-38
score: 134.1
IPR0022043-hydroxyisobutyrate dehydrogenase-related, conserved sitePROSITEPS008953_HYDROXYISOBUT_DHcoord: 48..61
IPR0089276-phosphogluconate dehydrogenase-like, C-terminal domain superfamilySUPERFAMILY481796-phosphogluconate dehydrogenase C-terminal domain-likecoord: 218..324
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 45..217

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CmaCh13G004860CmaCh13G004860gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh13G004860.1:exon:696CmaCh13G004860.1:exon:696exon
CmaCh13G004860.1:exon:695CmaCh13G004860.1:exon:695exon
CmaCh13G004860.1:exon:694CmaCh13G004860.1:exon:694exon
CmaCh13G004860.1:exon:693CmaCh13G004860.1:exon:693exon
CmaCh13G004860.1:exon:692CmaCh13G004860.1:exon:692exon
CmaCh13G004860.1:exon:691CmaCh13G004860.1:exon:691exon
CmaCh13G004860.1:exon:690CmaCh13G004860.1:exon:690exon
CmaCh13G004860.1:exon:689CmaCh13G004860.1:exon:689exon
CmaCh13G004860.1:exon:688CmaCh13G004860.1:exon:688exon


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh13G004860.1:three_prime_utrCmaCh13G004860.1:three_prime_utrthree_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh13G004860.1:cdsCmaCh13G004860.1:cds_9CDS
CmaCh13G004860.1:cdsCmaCh13G004860.1:cds_8CDS
CmaCh13G004860.1:cdsCmaCh13G004860.1:cds_7CDS
CmaCh13G004860.1:cdsCmaCh13G004860.1:cds_6CDS
CmaCh13G004860.1:cdsCmaCh13G004860.1:cds_5CDS
CmaCh13G004860.1:cdsCmaCh13G004860.1:cds_4CDS
CmaCh13G004860.1:cdsCmaCh13G004860.1:cds_3CDS
CmaCh13G004860.1:cdsCmaCh13G004860.1:cds_2CDS
CmaCh13G004860.1:cdsCmaCh13G004860.1:cdsCDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh13G004860.1:five_prime_utrCmaCh13G004860.1:five_prime_utrfive_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CmaCh13G004860.1CmaCh13G004860.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006551 leucine metabolic process
biological_process GO:0006574 valine catabolic process
molecular_function GO:0008442 3-hydroxyisobutyrate dehydrogenase activity
molecular_function GO:0051287 NAD binding
molecular_function GO:0050661 NADP binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0043621 protein self-association