CmaCh13G002140.1 (mRNA) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh13G002140.1 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 2.6e-29
Identity = 134/537 (24.95%), Postives = 230/537 (42.83%), Query Frame = 0

Query: 20  RWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINTLGFAKDLGSNIGIIAGLLA 79
           +W  + A + +    GT + F  YS  +K+    SQ ++N L  A DLG   G  +G+  
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 80  EVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFILVCFGTNSSNWANTAIMVT 139
              P  V+    A   F+G+ + WL IT  I  P +  +F+       S  W NTA  + 
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP-YSLVFLCCLLAGLSICWFNTACFIL 162

Query: 140 SVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPSNLVLLFAWLPSLLILIL-F 199
            +R+FP+ R + L L   + GI  A+ S  + AI  +   SNL LL   L SL+ L++ F
Sbjct: 163 CIRHFPNNRALALSLTVSFNGISAALYSLAFNAI--NPSSSNLYLL---LNSLVPLVVSF 222

Query: 200 PSIRPIRIR-------KHPNELKVFYQLLYVSILLAIFILFLTIAQKQVVFSQAGYVGGA 259
            ++ P+  +        + +     +    +++L  I    L ++      ++  ++G  
Sbjct: 223 AALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFIGAV 282

Query: 260 SFVVSLLCVPILIACREELLLY---KLNKQTAGPSVTVSMVHQNPPTLPASADSEIPEIS 319
             +V  LC P+L+  R+  L     +LN +++G  +      +N  T  +S      E  
Sbjct: 283 VLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGY--EHM 342

Query: 320 PSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQVGESLGYPQ 379
            +  E   N    G++ +    +   +  L  +A F G    L   +NLGQ+ +SLG   
Sbjct: 343 GTAKEG--NTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQNS 402

Query: 380 RAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCVGQLFIAYPFPG 439
                LV+  S F+FFGR+ S    + +  +++L R   F+ A L T +    +A     
Sbjct: 403 TT---LVTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQ 462

Query: 440 SVYL--AAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQLAVPLGSYILNVDVVGK 499
              L  A  +IG   G       ++ S++FG        N     +P+GS +    +   
Sbjct: 463 QTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGY-IAAS 522

Query: 500 LYDMEALREGKKIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRTREFYKR 544
           +Y+  A  +   I    + C G  C+  +F      ++ G V  L L  RT+  Y R
Sbjct: 523 IYEANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVYHR 564

BLAST of CmaCh13G002140.1 vs. ExPASy TrEMBL
Match: A0A6J1I5D7 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469398 PE=4 SV=1)

HSP 1 Score: 1131.3 bits (2925), Expect = 0.0e+00
Identity = 573/573 (100.00%), Postives = 573/573 (100.00%), Query Frame = 0

Query: 1   MGNKGGSEAWGFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60
           MGNKGGSEAWGFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT
Sbjct: 1   MGNKGGSEAWGFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFI 120
           LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFI
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPS 180
           LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPS
Sbjct: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPS 180

Query: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQV 240
           NLVLLFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 VFSQAGYVGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMVHQNPPTLPASA 300
           VFSQAGYVGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMVHQNPPTLPASA
Sbjct: 241 VFSQAGYVGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMVHQNPPTLPASA 300

Query: 301 DSEIPEISPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV 360
           DSEIPEISPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV
Sbjct: 301 DSEIPEISPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV 360

Query: 361 GESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCVGQL 420
           GESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCVGQL
Sbjct: 361 GESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCVGQL 420

Query: 421 FIAYPFPGSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQLAVPLGSYILN 480
           FIAYPFPGSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQLAVPLGSYILN
Sbjct: 421 FIAYPFPGSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQLAVPLGSYILN 480

Query: 481 VDVVGKLYDMEALREGKKIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRTREF 540
           VDVVGKLYDMEALREGKKIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRTREF
Sbjct: 481 VDVVGKLYDMEALREGKKIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRTREF 540

Query: 541 YKRDVYKNFNEEIWIPQTQMEFYRLDSKKSIED 574
           YKRDVYKNFNEEIWIPQTQMEFYRLDSKKSIED
Sbjct: 541 YKRDVYKNFNEEIWIPQTQMEFYRLDSKKSIED 573

BLAST of CmaCh13G002140.1 vs. ExPASy TrEMBL
Match: A0A6J1I2B3 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469248 PE=4 SV=1)

HSP 1 Score: 1109.7 bits (2869), Expect = 0.0e+00
Identity = 560/573 (97.73%), Postives = 564/573 (98.43%), Query Frame = 0

Query: 1   MGNKGGSEAWGFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60
           MGNKGGSEAWGFIKQVV GRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT
Sbjct: 1   MGNKGGSEAWGFIKQVVTGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFI 120
           LGFAKDLGSN+GIIAGLLAEVTPTWVLFI+GAFQNF GFFLIWLSITARIAKPKFWQMFI
Sbjct: 61  LGFAKDLGSNVGIIAGLLAEVTPTWVLFIVGAFQNFTGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPS 180
           LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQ YLAIYGHNDPS
Sbjct: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQLYLAIYGHNDPS 180

Query: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQV 240
           NLVLLFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 VFSQAGYVGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMVHQNPPTLPASA 300
           VFSQAGYVGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMVHQNPPTL ASA
Sbjct: 241 VFSQAGYVGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMVHQNPPTLSASA 300

Query: 301 DSEIPEISPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV 360
           DSEIPEI PSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV
Sbjct: 301 DSEIPEILPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV 360

Query: 361 GESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCVGQL 420
           GESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCVGQL
Sbjct: 361 GESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCVGQL 420

Query: 421 FIAYPFPGSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQLAVPLGSYILN 480
           FIAYPFPGSVY AAIIIGFGFGAQNPMLFAVISEMFGLR YSTLFNCGQL VPLGSYILN
Sbjct: 421 FIAYPFPGSVYFAAIIIGFGFGAQNPMLFAVISEMFGLRHYSTLFNCGQLTVPLGSYILN 480

Query: 481 VDVVGKLYDMEALREGKKIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRTREF 540
           VDVVGKLYDMEAL EGK+IAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRTREF
Sbjct: 481 VDVVGKLYDMEALHEGKQIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRTREF 540

Query: 541 YKRDVYKNFNEEIWIPQTQMEFYRLDSKKSIED 574
           YKRDVYKNFNEEIWIPQTQMEFYRLD+KKSIED
Sbjct: 541 YKRDVYKNFNEEIWIPQTQMEFYRLDNKKSIED 573

BLAST of CmaCh13G002140.1 vs. ExPASy TrEMBL
Match: A0A6J1HLT1 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111464780 PE=4 SV=1)

HSP 1 Score: 1088.2 bits (2813), Expect = 0.0e+00
Identity = 547/573 (95.46%), Postives = 560/573 (97.73%), Query Frame = 0

Query: 1   MGNKGGSEAWGFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60
           MGNKGGSEAW FIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT
Sbjct: 1   MGNKGGSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFI 120
           LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNF GFFLIWLSITARIAKPKFWQMFI
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPS 180
           LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAI+SQ YLAIYGHNDPS
Sbjct: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPS 180

Query: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQV 240
           NLVLLFAWLPSLLILILFPSIRPIRIRKHP+ELKVFYQLLYVSILLAIFILFLTIAQKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 VFSQAGYVGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMVHQNPPTLPASA 300
           VFSQAGYVGGASFVV LLCVPILIACREELLLYKLNK+TA PSV +SM+HQNPPTLP SA
Sbjct: 241 VFSQAGYVGGASFVVCLLCVPILIACREELLLYKLNKETAAPSVALSMLHQNPPTLPTSA 300

Query: 301 DSEIPEISPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV 360
           DSEIPEISPSCC NIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV
Sbjct: 301 DSEIPEISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV 360

Query: 361 GESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCVGQL 420
           GESLGY QRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTF+FAFLITCVGQL
Sbjct: 361 GESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAFLITCVGQL 420

Query: 421 FIAYPFPGSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQLAVPLGSYILN 480
           FIAYPFPGS+YLAAIIIGFGFGAQNPMLFAVISEMFGL+RYSTLFNCGQLA P GSYILN
Sbjct: 421 FIAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAAPFGSYILN 480

Query: 481 VDVVGKLYDMEALREGKKIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRTREF 540
           VDVVGKLYDMEALRE K+IAGKGLTCTGAHCFGGSFT+LAAATLFGAVVM VLAYRTREF
Sbjct: 481 VDVVGKLYDMEALRERKQIAGKGLTCTGAHCFGGSFTVLAAATLFGAVVMFVLAYRTREF 540

Query: 541 YKRDVYKNFNEEIWIPQTQMEFYRLDSKKSIED 574
           YKRDVYKNFNEEIWIPQT+MEFYRLD+KK+IED
Sbjct: 541 YKRDVYKNFNEEIWIPQTEMEFYRLDNKKNIED 573

BLAST of CmaCh13G002140.1 vs. ExPASy TrEMBL
Match: A0A6J1HP70 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111464775 PE=4 SV=1)

HSP 1 Score: 1087.0 bits (2810), Expect = 0.0e+00
Identity = 547/573 (95.46%), Postives = 559/573 (97.56%), Query Frame = 0

Query: 1   MGNKGGSEAWGFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60
           MGNKGGSEAW FIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT
Sbjct: 1   MGNKGGSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFI 120
           LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNF GFFLIWLSITARIAKPKFWQMFI
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPS 180
           LVCF TNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAI+SQ YLAIYGHNDPS
Sbjct: 121 LVCFATNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPS 180

Query: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQV 240
           NLVLLFAWLPSLLILILFPSIRPIRIRKHP+ELKVFYQLLYVSILLAIFILFLTIAQKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 VFSQAGYVGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMVHQNPPTLPASA 300
           VFSQAGYVGGASFVV LLCVPILIACREELLLYKLNKQTA PSV +SM+HQNPPTLP SA
Sbjct: 241 VFSQAGYVGGASFVVCLLCVPILIACREELLLYKLNKQTAAPSVALSMLHQNPPTLPTSA 300

Query: 301 DSEIPEISPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV 360
           DSEIPEISPSCC NIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV
Sbjct: 301 DSEIPEISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV 360

Query: 361 GESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCVGQL 420
           GESLGY QRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTF+FAFLITCVGQL
Sbjct: 361 GESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAFLITCVGQL 420

Query: 421 FIAYPFPGSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQLAVPLGSYILN 480
           FIAYPFPGS+YLAAIIIGFGFGAQNPMLFAVISEMFGL+RYSTLFNCGQLA P GSYILN
Sbjct: 421 FIAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAAPFGSYILN 480

Query: 481 VDVVGKLYDMEALREGKKIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRTREF 540
           VDVVGKLYDMEALRE K+IAGKGLTCTGAHCFGGSFT+LAAATLFGAVVM VLAYRTREF
Sbjct: 481 VDVVGKLYDMEALRERKQIAGKGLTCTGAHCFGGSFTVLAAATLFGAVVMFVLAYRTREF 540

Query: 541 YKRDVYKNFNEEIWIPQTQMEFYRLDSKKSIED 574
           YKRDVYKNFNEEIWIPQT+MEFYRLD+KK+IED
Sbjct: 541 YKRDVYKNFNEEIWIPQTEMEFYRLDNKKNIED 573

BLAST of CmaCh13G002140.1 vs. ExPASy TrEMBL
Match: A0A6J1HLS1 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111464770 PE=4 SV=1)

HSP 1 Score: 1086.6 bits (2809), Expect = 0.0e+00
Identity = 546/573 (95.29%), Postives = 560/573 (97.73%), Query Frame = 0

Query: 1   MGNKGGSEAWGFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60
           MGNKGGSEAW FIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT
Sbjct: 1   MGNKGGSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFI 120
           LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNF GFFLIWLSITARIAKPKFWQMFI
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPS 180
           LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAI+SQ YLAIYGHNDPS
Sbjct: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPS 180

Query: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQV 240
           NLVLLFAWLPSLLILILFPSIRPIRIRKHP+ELKVFYQLLYVSILLAIFILFLTIAQKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 VFSQAGYVGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMVHQNPPTLPASA 300
           VFSQAGYVGGASFVV LLCVPILIACREELLLYKLNK+TA PSV +SM+HQNPPTLP SA
Sbjct: 241 VFSQAGYVGGASFVVCLLCVPILIACREELLLYKLNKETAAPSVALSMLHQNPPTLPTSA 300

Query: 301 DSEIPEISPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV 360
           DSEIPEISPSCC NIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV
Sbjct: 301 DSEIPEISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV 360

Query: 361 GESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCVGQL 420
           GESLGY QRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTF+FAFLITCVGQL
Sbjct: 361 GESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAFLITCVGQL 420

Query: 421 FIAYPFPGSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQLAVPLGSYILN 480
           FIAYPFPGS+YLAAIIIGFGFGAQNPMLFAVISEMFGL+RYSTLFNCGQLA P GSYILN
Sbjct: 421 FIAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAAPFGSYILN 480

Query: 481 VDVVGKLYDMEALREGKKIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRTREF 540
           VDVVGKLYDMEALRE K+IAGKGLTCTGAHCFGGSFT+LAAATLFGAVVM VLAY+TREF
Sbjct: 481 VDVVGKLYDMEALRERKQIAGKGLTCTGAHCFGGSFTVLAAATLFGAVVMFVLAYQTREF 540

Query: 541 YKRDVYKNFNEEIWIPQTQMEFYRLDSKKSIED 574
           YKRDVYKNFNEEIWIPQT+MEFYRLD+KK+IED
Sbjct: 541 YKRDVYKNFNEEIWIPQTEMEFYRLDNKKNIED 573

BLAST of CmaCh13G002140.1 vs. NCBI nr
Match: XP_022970365.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima] >XP_022970399.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 1131.3 bits (2925), Expect = 0.0e+00
Identity = 573/573 (100.00%), Postives = 573/573 (100.00%), Query Frame = 0

Query: 1   MGNKGGSEAWGFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60
           MGNKGGSEAWGFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT
Sbjct: 1   MGNKGGSEAWGFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFI 120
           LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFI
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPS 180
           LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPS
Sbjct: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPS 180

Query: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQV 240
           NLVLLFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 VFSQAGYVGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMVHQNPPTLPASA 300
           VFSQAGYVGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMVHQNPPTLPASA
Sbjct: 241 VFSQAGYVGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMVHQNPPTLPASA 300

Query: 301 DSEIPEISPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV 360
           DSEIPEISPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV
Sbjct: 301 DSEIPEISPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV 360

Query: 361 GESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCVGQL 420
           GESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCVGQL
Sbjct: 361 GESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCVGQL 420

Query: 421 FIAYPFPGSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQLAVPLGSYILN 480
           FIAYPFPGSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQLAVPLGSYILN
Sbjct: 421 FIAYPFPGSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQLAVPLGSYILN 480

Query: 481 VDVVGKLYDMEALREGKKIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRTREF 540
           VDVVGKLYDMEALREGKKIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRTREF
Sbjct: 481 VDVVGKLYDMEALREGKKIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRTREF 540

Query: 541 YKRDVYKNFNEEIWIPQTQMEFYRLDSKKSIED 574
           YKRDVYKNFNEEIWIPQTQMEFYRLDSKKSIED
Sbjct: 541 YKRDVYKNFNEEIWIPQTQMEFYRLDSKKSIED 573

BLAST of CmaCh13G002140.1 vs. NCBI nr
Match: XP_022970240.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 1109.7 bits (2869), Expect = 0.0e+00
Identity = 560/573 (97.73%), Postives = 564/573 (98.43%), Query Frame = 0

Query: 1   MGNKGGSEAWGFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60
           MGNKGGSEAWGFIKQVV GRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT
Sbjct: 1   MGNKGGSEAWGFIKQVVTGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFI 120
           LGFAKDLGSN+GIIAGLLAEVTPTWVLFI+GAFQNF GFFLIWLSITARIAKPKFWQMFI
Sbjct: 61  LGFAKDLGSNVGIIAGLLAEVTPTWVLFIVGAFQNFTGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPS 180
           LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQ YLAIYGHNDPS
Sbjct: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQLYLAIYGHNDPS 180

Query: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQV 240
           NLVLLFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 VFSQAGYVGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMVHQNPPTLPASA 300
           VFSQAGYVGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMVHQNPPTL ASA
Sbjct: 241 VFSQAGYVGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMVHQNPPTLSASA 300

Query: 301 DSEIPEISPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV 360
           DSEIPEI PSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV
Sbjct: 301 DSEIPEILPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV 360

Query: 361 GESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCVGQL 420
           GESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCVGQL
Sbjct: 361 GESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCVGQL 420

Query: 421 FIAYPFPGSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQLAVPLGSYILN 480
           FIAYPFPGSVY AAIIIGFGFGAQNPMLFAVISEMFGLR YSTLFNCGQL VPLGSYILN
Sbjct: 421 FIAYPFPGSVYFAAIIIGFGFGAQNPMLFAVISEMFGLRHYSTLFNCGQLTVPLGSYILN 480

Query: 481 VDVVGKLYDMEALREGKKIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRTREF 540
           VDVVGKLYDMEAL EGK+IAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRTREF
Sbjct: 481 VDVVGKLYDMEALHEGKQIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRTREF 540

Query: 541 YKRDVYKNFNEEIWIPQTQMEFYRLDSKKSIED 574
           YKRDVYKNFNEEIWIPQTQMEFYRLD+KKSIED
Sbjct: 541 YKRDVYKNFNEEIWIPQTQMEFYRLDNKKSIED 573

BLAST of CmaCh13G002140.1 vs. NCBI nr
Match: XP_022964780.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata])

HSP 1 Score: 1088.2 bits (2813), Expect = 0.0e+00
Identity = 547/573 (95.46%), Postives = 560/573 (97.73%), Query Frame = 0

Query: 1   MGNKGGSEAWGFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60
           MGNKGGSEAW FIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT
Sbjct: 1   MGNKGGSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFI 120
           LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNF GFFLIWLSITARIAKPKFWQMFI
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPS 180
           LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAI+SQ YLAIYGHNDPS
Sbjct: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPS 180

Query: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQV 240
           NLVLLFAWLPSLLILILFPSIRPIRIRKHP+ELKVFYQLLYVSILLAIFILFLTIAQKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 VFSQAGYVGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMVHQNPPTLPASA 300
           VFSQAGYVGGASFVV LLCVPILIACREELLLYKLNK+TA PSV +SM+HQNPPTLP SA
Sbjct: 241 VFSQAGYVGGASFVVCLLCVPILIACREELLLYKLNKETAAPSVALSMLHQNPPTLPTSA 300

Query: 301 DSEIPEISPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV 360
           DSEIPEISPSCC NIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV
Sbjct: 301 DSEIPEISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV 360

Query: 361 GESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCVGQL 420
           GESLGY QRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTF+FAFLITCVGQL
Sbjct: 361 GESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAFLITCVGQL 420

Query: 421 FIAYPFPGSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQLAVPLGSYILN 480
           FIAYPFPGS+YLAAIIIGFGFGAQNPMLFAVISEMFGL+RYSTLFNCGQLA P GSYILN
Sbjct: 421 FIAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAAPFGSYILN 480

Query: 481 VDVVGKLYDMEALREGKKIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRTREF 540
           VDVVGKLYDMEALRE K+IAGKGLTCTGAHCFGGSFT+LAAATLFGAVVM VLAYRTREF
Sbjct: 481 VDVVGKLYDMEALRERKQIAGKGLTCTGAHCFGGSFTVLAAATLFGAVVMFVLAYRTREF 540

Query: 541 YKRDVYKNFNEEIWIPQTQMEFYRLDSKKSIED 574
           YKRDVYKNFNEEIWIPQT+MEFYRLD+KK+IED
Sbjct: 541 YKRDVYKNFNEEIWIPQTEMEFYRLDNKKNIED 573

BLAST of CmaCh13G002140.1 vs. NCBI nr
Match: XP_022964774.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata])

HSP 1 Score: 1087.0 bits (2810), Expect = 0.0e+00
Identity = 547/573 (95.46%), Postives = 559/573 (97.56%), Query Frame = 0

Query: 1   MGNKGGSEAWGFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60
           MGNKGGSEAW FIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT
Sbjct: 1   MGNKGGSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFI 120
           LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNF GFFLIWLSITARIAKPKFWQMFI
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPS 180
           LVCF TNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAI+SQ YLAIYGHNDPS
Sbjct: 121 LVCFATNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPS 180

Query: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQV 240
           NLVLLFAWLPSLLILILFPSIRPIRIRKHP+ELKVFYQLLYVSILLAIFILFLTIAQKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 VFSQAGYVGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMVHQNPPTLPASA 300
           VFSQAGYVGGASFVV LLCVPILIACREELLLYKLNKQTA PSV +SM+HQNPPTLP SA
Sbjct: 241 VFSQAGYVGGASFVVCLLCVPILIACREELLLYKLNKQTAAPSVALSMLHQNPPTLPTSA 300

Query: 301 DSEIPEISPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV 360
           DSEIPEISPSCC NIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV
Sbjct: 301 DSEIPEISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV 360

Query: 361 GESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCVGQL 420
           GESLGY QRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTF+FAFLITCVGQL
Sbjct: 361 GESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAFLITCVGQL 420

Query: 421 FIAYPFPGSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQLAVPLGSYILN 480
           FIAYPFPGS+YLAAIIIGFGFGAQNPMLFAVISEMFGL+RYSTLFNCGQLA P GSYILN
Sbjct: 421 FIAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAAPFGSYILN 480

Query: 481 VDVVGKLYDMEALREGKKIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRTREF 540
           VDVVGKLYDMEALRE K+IAGKGLTCTGAHCFGGSFT+LAAATLFGAVVM VLAYRTREF
Sbjct: 481 VDVVGKLYDMEALRERKQIAGKGLTCTGAHCFGGSFTVLAAATLFGAVVMFVLAYRTREF 540

Query: 541 YKRDVYKNFNEEIWIPQTQMEFYRLDSKKSIED 574
           YKRDVYKNFNEEIWIPQT+MEFYRLD+KK+IED
Sbjct: 541 YKRDVYKNFNEEIWIPQTEMEFYRLDNKKNIED 573

BLAST of CmaCh13G002140.1 vs. NCBI nr
Match: KAG6583526.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG6583533.1 Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG6583538.1 Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG6583544.1 Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1087.0 bits (2810), Expect = 0.0e+00
Identity = 547/573 (95.46%), Postives = 559/573 (97.56%), Query Frame = 0

Query: 1   MGNKGGSEAWGFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60
           MGNKGGSEAW FIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT
Sbjct: 1   MGNKGGSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFI 120
           LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNF GFFLIWLSITARIAKPKFWQMFI
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPS 180
           LVC GTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAI+SQ YLAIYGHNDPS
Sbjct: 121 LVCLGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPS 180

Query: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQV 240
           NLVLLFAWLPSLLILILFPSIRPIRIRKHP+ELKVFYQLLYVSILLAIFILFLTIAQKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 VFSQAGYVGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMVHQNPPTLPASA 300
           VFSQAGYVGGASFVV LLCVPILIACREELLLYKLNKQTA PSV +SM+HQNPPTLP SA
Sbjct: 241 VFSQAGYVGGASFVVCLLCVPILIACREELLLYKLNKQTAAPSVALSMLHQNPPTLPTSA 300

Query: 301 DSEIPEISPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV 360
           DSEIPEISPSCC NIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV
Sbjct: 301 DSEIPEISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV 360

Query: 361 GESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCVGQL 420
           GESLGY QRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTF+FAFLITCVGQL
Sbjct: 361 GESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAFLITCVGQL 420

Query: 421 FIAYPFPGSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQLAVPLGSYILN 480
           FIAYPFPGS+YLAAIIIGFGFGAQNPMLFAVISEMFGL+RYSTLFNCGQLA P GSYILN
Sbjct: 421 FIAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAAPFGSYILN 480

Query: 481 VDVVGKLYDMEALREGKKIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRTREF 540
           VDVVGKLYDMEALRE K+IAGKGLTCTGAHCFGGSFT+LAAATLFGAVVM VLAYRTREF
Sbjct: 481 VDVVGKLYDMEALRERKQIAGKGLTCTGAHCFGGSFTVLAAATLFGAVVMFVLAYRTREF 540

Query: 541 YKRDVYKNFNEEIWIPQTQMEFYRLDSKKSIED 574
           YKRDVYKNFNEEIWIPQT+MEFYRLD+KK+IED
Sbjct: 541 YKRDVYKNFNEEIWIPQTEMEFYRLDNKKNIED 573

BLAST of CmaCh13G002140.1 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 570.9 bits (1470), Expect = 1.2e-162
Identity = 299/583 (51.29%), Postives = 398/583 (68.27%), Query Frame = 0

Query: 5   GGSEAWGFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINTLGFA 64
           G  E   F+     GRWF VFA  ++M   G TY+F TYSK IK+   Y QT +N LGF 
Sbjct: 2   GNDETVRFLIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFF 61

Query: 65  KDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFILVCF 124
           KDLG+N+G+++GL+AEVTPTW +  IG+  NF+G+F+IWL++T ++AKPK WQM + +C 
Sbjct: 62  KDLGANVGVLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICI 121

Query: 125 GTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPSNLVL 184
           G NS N+ANT  +VT V+NFP+ RG++LGLLKGYVG+ GAI +Q Y AIYGH D  +L+L
Sbjct: 122 GANSQNFANTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGH-DSKSLIL 181

Query: 185 LFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQVVFSQ 244
           L AWLP+ + L+    IR  ++ +  NEL VFYQ LY+SI LA+F++ + IA+KQV FS+
Sbjct: 182 LIAWLPAAVSLVFVYLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSK 241

Query: 245 AGYVGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMVHQNPPTLPASADSE- 304
           A Y   A+   +LL VP+ ++ ++EL ++ + K    P    S V    P      D + 
Sbjct: 242 AAYAASATICCALLFVPLTVSVKQELEVWNMMKL---PIEEPSEVKVEKPKKELDLDQDK 301

Query: 305 ------IPEISPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNL 364
                   + + SC   +++ P RGED+TILQA+ S DM ++ +ATF G GSSL A+DNL
Sbjct: 302 AAKVNGEEKETKSCFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNL 361

Query: 365 GQVGESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCV 424
           GQ+GESLGYP   +   VS VSI+N+FGRVFSGF+SE L+ KYKLPRPL  +   L++C 
Sbjct: 362 GQIGESLGYPNHTVSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCA 421

Query: 425 GQLFIAYPFPGSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQLAVPLGSY 484
           G L IA+P PGSVY+A+I++GF FGAQ P+LFA+ISE+FGL+ YSTLFNCGQLA PLGSY
Sbjct: 422 GHLLIAFPVPGSVYIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSY 481

Query: 485 ILNVDVVGKLYDMEALREGKKIAGKG--------LTCTGAHCFGGSFTILAAATLFGAVV 544
           ILNV V G LYD EAL   K++  +G        LTC G+ C+   F ILAA T FGA+V
Sbjct: 482 ILNVRVTGMLYDKEAL---KQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALV 541

Query: 545 MLVLAYRTREFYKRDVYKNFNEEIWIPQTQMEFYRLDSKKSIE 573
            L LA RTREFYK D+YK F E    P+++ E    DS+K++E
Sbjct: 542 SLGLAIRTREFYKGDIYKKFRES---PESESELVP-DSRKAVE 573

BLAST of CmaCh13G002140.1 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 520.4 bits (1339), Expect = 1.8e-147
Identity = 262/560 (46.79%), Postives = 373/560 (66.61%), Query Frame = 0

Query: 15  QVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINTLGFAKDLGSNIGII 74
           Q++ GRWF  F  L++M   G TY+F  YS  IK    Y QT +N L F KDLG+N+G++
Sbjct: 16  QILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGVL 75

Query: 75  AGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFILVCFGTNSSNWANT 134
           AGLL EVTP W + +IGA  NF G+F+IWL++T RI+KP+ W M + +C G NS ++ANT
Sbjct: 76  AGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFANT 135

Query: 135 AIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPSNLVLLFAWLPSLLI 194
             +VT V+NFP+ RG++LG+LKGYVG+ GAI++Q Y A YG  D   L+L+  WLP+++ 
Sbjct: 136 GSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYG-EDTKELILMIGWLPAIVS 195

Query: 195 LILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQVVFSQAGYVGGASFV 254
                +IR +++++  NELKVFY  LY+S+ LA F++ + I  K   F+Q+ + G A+ V
Sbjct: 196 FAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGSAAVV 255

Query: 255 VSLLCVPILIACREELLLYKLNKQTAGPSVTVSMVHQNPPTLPASADSEIPEIS------ 314
           + LL +PI++   EE  L+K  +        +++V + P    +    +  E S      
Sbjct: 256 IVLLLLPIIVVILEEKKLWKEKQVALNDPAPINVVTEKPKLDSSEFKDDDGEESKEVVEK 315

Query: 315 ---PSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQVGESLG 374
              PSC   ++N PERG+D+TILQA+FS DM ++ LAT  G G +L AIDNLGQ+G SLG
Sbjct: 316 VKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLGQIGNSLG 375

Query: 375 YPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCVGQLFIAYP 434
           YP+R++   VS VSI+N++GRV SG +SE  + KYK PRPL  +   L++C G L IA+ 
Sbjct: 376 YPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAGHLLIAFN 435

Query: 435 FPGSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQLAVPLGSYILNVDVVG 494
            PG +Y+A++IIGF FGAQ P+LFA+ISE+FGL+ YSTL+N G +A P+GSY+LNV V G
Sbjct: 436 VPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYLLNVRVAG 495

Query: 495 KLYDMEALREGK-----KIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRTREF 554
            LYD+EA ++ K     ++ G+ L C G  CF  SF I+AA TLFG +V +VL  RT++F
Sbjct: 496 YLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMVLVIRTKKF 555

Query: 555 YKRDVYKNFNEEIWIPQTQM 561
           YK D+YK F E+    + +M
Sbjct: 556 YKSDIYKKFREKALAAEMEM 574

BLAST of CmaCh13G002140.1 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 300.4 bits (768), Expect = 3.0e-81
Identity = 192/561 (34.22%), Postives = 295/561 (52.58%), Query Frame = 0

Query: 17  VAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINTLGFAKDLGSNIGIIAG 76
           +  RW    A + +    G  Y+F + S VIK+  +Y+Q +++ LG AKDLG ++G IAG
Sbjct: 13  INNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKDLGDSVGFIAG 72

Query: 77  LLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFILVCFGTNSSNWANTAI 136
            L+E+ P W   ++GA QN IG+  +WL +T R      W M +L+  G N   + NT  
Sbjct: 73  TLSEILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGNNGETYFNTGA 132

Query: 137 MVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPSNLVLLFAWLPSLLILI 196
           +V+ V+NFP  RG ++G+LKG+ G+GGAI+SQ Y  I+  N P++L+L+ A  P+++++ 
Sbjct: 133 LVSGVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHSSN-PASLILMVAVTPAVVVVC 192

Query: 197 LFPSIRPIRIRK--HPNELKVFYQLLYVSILLAIFILFLTIAQKQVVFSQAGYVGGASFV 256
           L   IRP+   K   P +   F  +  V +LLA +++ + + Q  VV S          +
Sbjct: 193 LMFFIRPVGGHKQIRPTDGASFTFIYGVCLLLAAYLMSVMLIQDLVVVSHNVITVFTIVL 252

Query: 257 VSLLCVPILIACR--------------EELLLYKLNKQTAG---PSVTVSMVHQNPP--- 316
             +L VPIL+                 EE L+ K   Q  G   P + +S V    P   
Sbjct: 253 FVILVVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQTPDLILSEVEDEKPKDV 312

Query: 317 -TLPAS-ADSEIPEISPSCCE---------NIWNKPERGEDFTILQAVFSKDMALICLAT 376
             LPAS     I  +     +         N    P RGEDFT+ QA+   D  LI  + 
Sbjct: 313 DLLPASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALVKADFWLIFFSL 372

Query: 377 FSGCGSSLAAIDNLGQVGESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLP 436
             G GS L  IDNLGQ+ +SLGY      +LVS +SI+NF GR+  G+ SE ++  Y  P
Sbjct: 373 LLGSGSGLTVIDNLGQMSQSLGYDN--THVLVSMISIWNFLGRIGGGYFSELVVRDYAYP 432

Query: 437 RPLTFSFAFLITCVGQLFIAYPFPGSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYST 496
           RP+  + A LI  VG +F AY +PG++Y+  ++IG G+GA   ++ A  SE+FGL+++  
Sbjct: 433 RPVAMAVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAIVPATASELFGLKKFGA 492

Query: 497 LFNCGQLAVPLGSYILNVDVVGKLYDMEALREGKKIA---GKGLTCTGAHCFGGSFTILA 542
           L+N   LA P GS + +  +   +YD EA R+           L C G+ CF  +  I++
Sbjct: 493 LYNFLTLANPAGSLVFSGMIASSIYDREAERQAHGSVFDPDDALRCNGSICFFLTSLIMS 552

BLAST of CmaCh13G002140.1 vs. TAIR 10
Match: AT5G50630.1 (Major facilitator superfamily protein )

HSP 1 Score: 290.4 bits (742), Expect = 3.1e-78
Identity = 176/537 (32.77%), Postives = 293/537 (54.56%), Query Frame = 0

Query: 13  IKQVVAGRWFSVFAGLIMMIGNGTTYVF-PTYSKVIKTQFDYSQTQINTLGFAKDLGSNI 72
           ++ +V  RW      + +    G  Y+F  + S  IKT   Y+Q QI  LG AK+LG  I
Sbjct: 9   LELLVNDRWLVFVCAMWVQSVAGVGYLFGGSMSPAIKTSLGYNQKQIALLGVAKNLGDAI 68

Query: 73  GIIAGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFILVCFGTNSSNW 132
           G ++G L+EV+P+WV+ ++GA QN  G+ ++WL +T ++     W +F+ +  GTN   +
Sbjct: 69  GFVSGALSEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTNGETY 128

Query: 133 ANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPSNLVLLFAWLPS 192
            NTA +V+ + NFP+ RG ++G+LKG+ G+ GAI++Q YL ++  +  S+++L+ A  P 
Sbjct: 129 YNTASLVSCIHNFPESRGPVVGILKGFSGLSGAILTQVYL-MFNPSHDSSVILMVALGPP 188

Query: 193 LLILILFPSIRPI----RIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQVVFSQAGY 252
           +++L L   +RP+    R     ++L+ F  +    ++LA+++L L + Q     +Q   
Sbjct: 189 VVVLALLFIVRPVERSCRTNLRSDDLR-FLAIYGFCVVLAVYLLGLLVLQSVFDMTQTII 248

Query: 253 VGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMVHQNPPTLPASADSEIPEI 312
               + +V  + VP+L+     + +   N  +  P    S V Q+            PE 
Sbjct: 249 TTSGAILVIFMVVPVLVPF-SSVFISGNNVTSVKPEEGTSNVDQHEARTLIERSDRPPEK 308

Query: 313 SPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQVGESLGYP 372
             + C         GEDFT+LQA+   D  LI ++   G GS +  IDNLGQ+  SLGY 
Sbjct: 309 KRAPC--------IGEDFTLLQALGQADFWLIFMSLVLGVGSGITIIDNLGQICYSLGYS 368

Query: 373 QRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCVGQLFIAYPFP 432
                I VS +SI NF GRV  G+ SE ++ K  LPR L  S    I  +G ++ A  +P
Sbjct: 369 NTK--IFVSLISISNFLGRVAGGYFSELIIRKLSLPRTLAMSVVQAIMSLGLIYYAIDWP 428

Query: 433 GSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQLAVPLGSYILNVDVVGKL 492
           G +Y+  I+IG G+GA   +  A +S++FGL+ + +L+N    A+P+GS++ +  +   +
Sbjct: 429 GKIYVVTIVIGMGYGAHWAIAPASVSDIFGLKSFGSLYNFQITALPIGSFVFSGVIASNI 488

Query: 493 YDMEALRE-GKKIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRTREFYKR 544
           YD  A ++ G     + L CTG+ C+  + ++++   L   V+ L + YRTR+FY R
Sbjct: 489 YDYYARKQAGPTTETESLVCTGSVCYSVTCSLMSMLCLMAMVLSLSVVYRTRKFYLR 532

BLAST of CmaCh13G002140.1 vs. TAIR 10
Match: AT5G50520.1 (Major facilitator superfamily protein )

HSP 1 Score: 290.4 bits (742), Expect = 3.1e-78
Identity = 176/537 (32.77%), Postives = 293/537 (54.56%), Query Frame = 0

Query: 13  IKQVVAGRWFSVFAGLIMMIGNGTTYVF-PTYSKVIKTQFDYSQTQINTLGFAKDLGSNI 72
           ++ +V  RW      + +    G  Y+F  + S  IKT   Y+Q QI  LG AK+LG  I
Sbjct: 9   LELLVNDRWLVFVCAMWVQSVAGVGYLFGGSMSPAIKTSLGYNQKQIALLGVAKNLGDAI 68

Query: 73  GIIAGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFILVCFGTNSSNW 132
           G ++G L+EV+P+WV+ ++GA QN  G+ ++WL +T ++     W +F+ +  GTN   +
Sbjct: 69  GFVSGALSEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTNGETY 128

Query: 133 ANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPSNLVLLFAWLPS 192
            NTA +V+ + NFP+ RG ++G+LKG+ G+ GAI++Q YL ++  +  S+++L+ A  P 
Sbjct: 129 YNTASLVSCIHNFPESRGPVVGILKGFSGLSGAILTQVYL-MFNPSHDSSVILMVALGPP 188

Query: 193 LLILILFPSIRPI----RIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQVVFSQAGY 252
           +++L L   +RP+    R     ++L+ F  +    ++LA+++L L + Q     +Q   
Sbjct: 189 VVVLALLFIVRPVERSCRTNLRSDDLR-FLAIYGFCVVLAVYLLGLLVLQSVFDMTQTII 248

Query: 253 VGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMVHQNPPTLPASADSEIPEI 312
               + +V  + VP+L+     + +   N  +  P    S V Q+            PE 
Sbjct: 249 TTSGAILVIFMVVPVLVPF-SSVFISGNNVTSVKPEEGTSNVDQHEARTLIERSDRPPEK 308

Query: 313 SPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQVGESLGYP 372
             + C         GEDFT+LQA+   D  LI ++   G GS +  IDNLGQ+  SLGY 
Sbjct: 309 KRAPC--------IGEDFTLLQALGQADFWLIFMSLVLGVGSGITIIDNLGQICYSLGYS 368

Query: 373 QRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCVGQLFIAYPFP 432
                I VS +SI NF GRV  G+ SE ++ K  LPR L  S    I  +G ++ A  +P
Sbjct: 369 NTK--IFVSLISISNFLGRVAGGYFSELIIRKLSLPRTLAMSVVQAIMSLGLIYYAIDWP 428

Query: 433 GSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQLAVPLGSYILNVDVVGKL 492
           G +Y+  I+IG G+GA   +  A +S++FGL+ + +L+N    A+P+GS++ +  +   +
Sbjct: 429 GKIYVVTIVIGMGYGAHWAIAPASVSDIFGLKSFGSLYNFQITALPIGSFVFSGVIASNI 488

Query: 493 YDMEALRE-GKKIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRTREFYKR 544
           YD  A ++ G     + L CTG+ C+  + ++++   L   V+ L + YRTR+FY R
Sbjct: 489 YDYYARKQAGPTTETESLVCTGSVCYSVTCSLMSMLCLMAMVLSLSVVYRTRKFYLR 532