CmaCh09G009540.1 (mRNA) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh09G009540.1
Type	mRNA
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Ubiquitin thioesterase OTU1
Location	Cma_Chr09: 4795337 .. 4801100 (-)
Sequence length	1418
RNA-Seq Expression	CmaCh09G009540.1
Synteny	CmaCh09G009540.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGTACTAAGCTTTAATGAAATTCTTCAACCCTTTTCATCTCTCTATCAATTCTTCTTCTCATCTTACCATGTTGAGGTTGGTTATCGAGTTGAAGCACCACCTGGTTTTCGATAAAGCTCTTTGGTTCTTCGTCGAAGCTTTGAACGCGATCGCTTTCGCTTTGTTTCTATGCCTTCTTGCTCTAGGTATGTCTCGTCTCAAGAAATTATTCGAATAGCTATAACAATACAGGTTAGAGAAATACACGATAACCATACGAGTTATGGTAGTTGGGAGGAGACGACTATATTGAATAATTTTTTGTTTCTATCTCTTGAATTTGTAGTTGGCGCATCGGTGGGAACGGTGATCGGAGCTTTCAAAGGCCATGGCGCTGCCATAGGGGCTATAGTCGGTGCAGCCGCAGCCCTTCAGCTATTGAGCTGCCCCGAACCGGAGTATAGATATTCTTTGTCAAAGGTAGGGTTTTCAATATTAAAGGTTCAAGTTCTTACTTGTTATTAAATTATAAGTTTACGTTCGTAAATCAAATACTTTTAAAAAATTTACGAATTAAATAAACCAAAGTTGAGGGACTAAAAGTGTAATTTAACTAAATTTAAATTTTTTTATGGTGGCAGGAAGCAATAATGGAGAGTCTAATGAATGGGAAGGGGTTGATGAACTGGGTAAGTGAAAAAGCTTGAATGCTGCCATTAATGGCGGATTTCAAGATCCTTCATATTTCTAAAGCAAAACAAAATTTTCAGGCGAGCAACTTCTCGGAGTATATGTACCGAGAGATGTCGGAGTTCGTCGACGTCGCCGGAGAAAGCTGTGTGGAAGGGATGTCAATGGAGAGTGTTCAAAACTTGCCTATTTGTGAATATGATCATAGCAAGATGAAGAACTCCATTTCTTGTCCCATTTGCTTAGAGGTAATACATACATACATACATACATATATATACATACATACATCTCATACATACATACATATATATACATACATACATACATACATATATATACATACATACATACATACATATATATATATATACATACATACTTATATATATATATATATATATATATATATATATATATATATATATTATTTTTGTTTTTTTTTTGGTTTTTCTTTTTGGTTTCTTAAATTTCTTATAATTTCAAAAAAATTAAAAAAAAAATAATTACATTTTTTTAAAAATGAAAAAGAAAAACTAAGAGTTTAAAAAAATGTAAATTAATTATTTCGTTGGCTTGAAAATATATTTTATTGAATATCTTAACTTTAATTTCAATTTTAAATTTATTTTATATTTGTTTAAGAATTTATTAAATATAATTATTTAAAATATACGTATTGTATAGTATGACTAAGAAAGTTGACCAAACTTTAAATATGAGTTTTATTATTGTTGTTATGAATAATTAATATTGTTGACCTAACTAAAAATAAATTAAAAGAAATTTAATATCGTTAAACTCGAAATTTTGAATTGATTTAGTTAGTGATTCAAACAACTCGAATAGAGTTCACAACTCAACACTCAAACATTATGTTGGGTTGGATTGTCCGGTTGTTTTTGTTTTTTTCAAAAAAAAATTATACTTATCCACAACACATGTTATATTAATAATTAAAATATCATAAAATTCAAATATCGTGCATTCACAAATCATAACGTATTGCTAACATCGATTACAAATGTTAAATATTCTTAATTTTCATAATAATCTTAAAAGTAGGGTAGTGTAGGGTTTGTTGTAGCTCTATTTTGTGGTTGGTCGGGTTGCCTCGAAAAAAAATGTAAACCCAGGAACTAATCCGAAGTTTTCGATCTTTAAAAAATTCAACCCAACCGACAATTCGAATTGATCAAGTTCTTGGGTCATATGAATACCCGTTAATTATCTCGTTTTTATTAATTATTATCGAGTAAATTATATATTAATTAATTAATAATTATTATTATTATTTTTATTTTTCAGGAATTTGAAGTTGGAGAGTTTGGTAGGAAACTTCCAAATTGTGATCATTTTTTTCATGTGGATTGCATAGATCAATGGCTACGATTGAATGGATGTTGCCCATTTTGTAGAAAATTTTGTAATTAAACCATACCTCATAATATTCTCAAATTAATTTTTTTTTTTAAGTCACCGTCATTATTAATATTTGAAAAAATATATTAAAAATAAATTAATTGAAATTATGATAATCTCTTAGCGAATTTTATACGCTAATATTTTAAATTCATATATTTTTTTTTAGCTTTTTTTAATATTTAAAAATATAATTTTTAAAATTGTTTGTAAGAAATATTTATTTATTTGTTTAATTTAACATTTAAAATAATCTTTATTTATTTTTATAAATATGATAATTGATGCTTTATATTTGGCCCTTCCTTTTTGACTTTTCAATGATGTGAAAGTACGATTTTACCCTTGTTTTAAAAATGTTTTCAAAAAGTTTTATTATTATTATTATTATTTGGCTCATTTGAAGGAGATTTGGGTTTTTGAAACTAAAAATTTAAACCTTCTTTAAGAAAATGACTCAATTCTAAGTGGATTAGATAAATTCGATTAAAATTTGTAAAGTTAAAGTAAATTATGGTTAAAATTCATGTATTTTAGCCAAATTTTGATTCAACTTCATCAACTCACTCGACGATAGAAAGTCGAGTTATGTTGTGAACTCTATTTAGAAGGACGAGGAAAGTTCTCCATAGGAAACCCTTGATGCAGCTCATTTTTATGGCTTTTGTGTGGTCTAAGCTTTTATCCTTTTGAACAAAATTACATGTTAAATGTTGTTCTATCTGTGAGGTGCTAGATCGGTTGGAGAGTGGAACGAAACATTGTCTCTAAGGGTGTGGAAATTTCTCCCTAATAGATGTGTTTTAACACCTTGAGGGAAAGTCCGAAAAGGAAAGCTCAAAGAGGACAATATCTGCTAGTGATGGGCTTGGATTGTTTCAAATGGTATTAGAGCCCGACACCGGATGATGTGTCAATGAGGGAGCTAAACCCTGAAGGGGGTGGACACCAGGCGGTGTGCCAACGAGGACGCTGGCCTCAAAAGGGGTGGATTGGAGGGTCTTACATCGATTCGAGAAGGGAACGAGTGTCAGTGAGGATGTTGTGTCACAAAGGGGGTGGATTGTGAGATCGCACATCGGTTGGAGAAGGGAGCGAAACATTGTTTATAAGGGCGTGGGAACTTCTCTCTAGTAGACGCATTTTAAAACCTTGAGGGAAAGCCTGGAAGGAAAAGCCTAAAAAGGGCAATATCTTCTAGCGGTAGGTTTGAGTGGTTACAAATGGTATCAGAGCCAGACACCGGGCGATGTGCCAGCAAGGAGGCTGAACTTTGAAGGGGGTGGACACCAGGCGGTGTGCCAGTGAGGATGTTGGGTTCCAAAGGGGGACGAAATATTCTTTACAAGGGTGTGGAAACCTCTTTCTAGTAGATAGCATTTTAAAACCTTGAGGGGAAGTCTATAAGAGAAAACCCAAAGAAGACAATATCTGCTAACGATAGGCTTAGACTGTTGCACTAGAGAAGTTTTTTTGTACTATGTTAACATACAATCTCTCTTTTGTATCATTTCATTTCATCAATGAAATTGTTTCGTATCAGAAAATAAACCTCCGCCGGCGGTTTGTGACCCCACGAGTCAGATTAAGAGAAAAAGTTTAAAGATAAAGATCAAGTTCTTGACATATAACTTCTTAATTGTTTCTAAAAGTATGGGGCTCTCTCTTGTCTGTAGCTATGCCTCATGTTCATTTTTGTGCTACTTCTGTTGCAATGTACATTAGGTATGTTATGGACCACGATAAGCACAAGGCTGCCGAGCTAAGACAGGTTCGTTCTTATTTATAGTGTTTCGTCGTTATTGGAACTACCCTACATTATACTGCTTTCTATCATATCTTTCTTGAAGTTCATATTTGTATTCTTCACTCGGAACATAAGCCACAATATGATTCTCTTGAAGGTCATAGCTGCAACAGTAGCAAGTGATCCAACAAGGTATTGTGAAGCGTTCCCCACTGTCTTGTGTTTCTAGCCTTAGATGATGGGCTAAGTGTTTTGTTGAACTTCCTCATGGATTTCTTGAATATGTTGCTTTGTGTCACAATCGCGCTCTTACGGGACGTTTGTAAGCGATTGTGCAGCATTCACTCTCACTACTGAGTGAGTCAAATTGTCTCGCCTTCAGCCCTACGATATATACCCATGTCTTAGACCTTGTTTGTAAGTAAGATTTGAGAGAAATGAAGAAAGCAATGTTATAGGTCATAAACGAAAAAGGCAATACAATAGCTTAGATAACATATAGCTCCGGGTAGAAAGAATTGATGTCTAGTCGCATCCATCCTCCAAGTGAGATATTTCAAGTCTACCAAGCGTGGACATAATTATGTTGAAACGTCTTACACTTAGAAAATACAATGAAGAAACTATACATCATGAAAGGGGTAAAGGAAAGCAATAAAGTGATACAACACAAGGGTTTTGACATGCATCGGGCATGCAGCCATGTGCAACACGCCTTGGACAAGCATAACTGTGACATTTTGCATGTTTGCTGCACTCTTTGTTCTTCTAATAGTAAACTGTAAACCAAACCGAAACATAAGAAAGCTTTAGGTGTAGCTGGACTGATATCAAATCTATTTCAGTTCGTTTGTTCTTTTTAATCTCATTTTCTCTTACACAGGTGCAATTGAGCTTTCGATACTATTCGAGTATTATGGGCTTGAAATTGCAGCTTACGATATCCAGACCAAACGATGTGATTTGTATGGTCAGGTTAGGATTTTCAGTTCCTATGACCCCAAGAGTAATCCTTTCACCGTTGACAGGAAAGGAAGTATTCAGAAAGGGTTCTGCTGATATATGATGGACTCCACTATGATGCTTTAGTTGTATGATTTTCTGATTCCCTCAAATATAATAAAGCAAGATGACCTTGATAATATGTTCTTTTGTATGAACAGTACCTTATAAAGCATGATCCCTTTGTCTTTGATTGTGTCTGATATATGAATCTTGGGTTCCTGCATCTTTGTTCTTGTCAGATGTCTCCATCTGAGGGAGCTCCAGAGGAATTTGATCAGACCATATTTTCTGTGAATCGGGACAGGACAATTGGTCCCATTGAAGGGCAGGCTCTTCATTTTGTTGAAGAACAGCAAAGGTAGGATCTTGTGTAATGATGAAAGAAACTTGAAGAACTCTTTGAATGTTTCGGCTTCCTGCATCTTAATATACGCTTGCACGTTTCAGGAAACGGAGATTCACCGACACGGCCAACTTCACGCTGCGATGTGGGGTTTGCCAAATCGGAGTCATCGGCCAGACGGTAGTCCTTGTCGAAATTCATTGCTTGCTTCATTAATTCTTCAAACTCTTGTAATTCATTTAGAGAATCCAATGTTCAGGAGGCTGTAGAACACGCAAAAGCCACTGGCCATGTCAACTTCCAAGAGTATAGATGACAGGAAGAATGTTTGACAAAACAATTGAGCTGTTCGGTTTCAGCTATATCGACCTCAACTCCTTCGGCTTGTTGAATTGCTGTGGCATTCTAATTGTCATATTACCATGAATGTTTCATATTTACTGACCAGTGTTGTTCATATTGTGATCTCTTGGGACTGAGCTTCTTTTCTTTGTTCTTGTCCCTTGCTTGTCGATAACATCATATGTTCTTTTACAAAATTTCTAAACTATGGTACTTTCCAAAATATAAGATCAG

mRNA sequence

CAGTACTAAGCTTTAATGAAATTCTTCAACCCTTTTCATCTCTCTATCAATTCTTCTTCTCATCTTACCATGTTGAGGTTGGTTATCGAGTTGAAGCACCACCTGGTTTTCGATAAAGCTCTTTGGTTCTTCGTCGAAGCTTTGAACGCGATCGCTTTCGCTTTGTTTCTATGCCTTCTTGCTCTAGTTGGCGCATCGGTGGGAACGGTGATCGGAGCTTTCAAAGGCCATGGCGCTGCCATAGGGGCTATAGTCGGTGCAGCCGCAGCCCTTCAGCTATTGAGCTGCCCCGAACCGGAGTATAGATATTCTTTGTCAAAGGAAGCAATAATGGAGAGTCTAATGAATGGGAAGGGGTTGATGAACTGGGCGAGCAACTTCTCGGAGTATATGTACCGAGAGATGTCGGAGTTCGTCGACGTCGCCGGAGAAAGCTGTGTGGAAGGGATGTCAATGGAGAGTGTTCAAAACTTGCCTATTTGTGAATATGATCATAGCAAGATGAAGAACTCCATTTCTTGTCCCATTTGCTTAGAGGAATTTGAAGTTGGAGAGTTTGGTAGGAAACTTCCAAATTGTGATCATTTTTTTCATGTGGATTGCATAGATCAATGGCTACGATTGAATGGATGTTGCCCATTTTGTAGAAAATTTTCTATGCCTCATGTTCATTTTTGTGCTACTTCTGTTGCAATGTACATTAGGTATGTTATGGACCACGATAAGCACAAGGCTGCCGAGCTAAGACAGGTCATAGCTGCAACAGTAGCAAGTGATCCAACAAGGTATTGTGAAGCGTTCCCCACTGTCTTGTGTGCAATTGAGCTTTCGATACTATTCGAGTATTATGGGCTTGAAATTGCAGCTTACGATATCCAGACCAAACGATGTGATTTGTATGGTCAGATGTCTCCATCTGAGGGAGCTCCAGAGGAATTTGATCAGACCATATTTTCTGTGAATCGGGACAGGACAATTGGTCCCATTGAAGGGCAGGCTCTTCATTTTGTTGAAGAACAGCAAAGGAAACGGAGATTCACCGACACGGCCAACTTCACGCTGCGATGTGGGGTTTGCCAAATCGGAGTCATCGGCCAGACGGAGGCTGTAGAACACGCAAAAGCCACTGGCCATGTCAACTTCCAAGAGTATAGATGACAGGAAGAATGTTTGACAAAACAATTGAGCTGTTCGGTTTCAGCTATATCGACCTCAACTCCTTCGGCTTGTTGAATTGCTGTGGCATTCTAATTGTCATATTACCATGAATGTTTCATATTTACTGACCAGTGTTGTTCATATTGTGATCTCTTGGGACTGAGCTTCTTTTCTTTGTTCTTGTCCCTTGCTTGTCGATAACATCATATGTTCTTTTACAAAATTTCTAAACTATGGTACTTTCCAAAATATAAGATCAG

Coding sequence (CDS)

ATGAAATTCTTCAACCCTTTTCATCTCTCTATCAATTCTTCTTCTCATCTTACCATGTTGAGGTTGGTTATCGAGTTGAAGCACCACCTGGTTTTCGATAAAGCTCTTTGGTTCTTCGTCGAAGCTTTGAACGCGATCGCTTTCGCTTTGTTTCTATGCCTTCTTGCTCTAGTTGGCGCATCGGTGGGAACGGTGATCGGAGCTTTCAAAGGCCATGGCGCTGCCATAGGGGCTATAGTCGGTGCAGCCGCAGCCCTTCAGCTATTGAGCTGCCCCGAACCGGAGTATAGATATTCTTTGTCAAAGGAAGCAATAATGGAGAGTCTAATGAATGGGAAGGGGTTGATGAACTGGGCGAGCAACTTCTCGGAGTATATGTACCGAGAGATGTCGGAGTTCGTCGACGTCGCCGGAGAAAGCTGTGTGGAAGGGATGTCAATGGAGAGTGTTCAAAACTTGCCTATTTGTGAATATGATCATAGCAAGATGAAGAACTCCATTTCTTGTCCCATTTGCTTAGAGGAATTTGAAGTTGGAGAGTTTGGTAGGAAACTTCCAAATTGTGATCATTTTTTTCATGTGGATTGCATAGATCAATGGCTACGATTGAATGGATGTTGCCCATTTTGTAGAAAATTTTCTATGCCTCATGTTCATTTTTGTGCTACTTCTGTTGCAATGTACATTAGGTATGTTATGGACCACGATAAGCACAAGGCTGCCGAGCTAAGACAGGTCATAGCTGCAACAGTAGCAAGTGATCCAACAAGGTATTGTGAAGCGTTCCCCACTGTCTTGTGTGCAATTGAGCTTTCGATACTATTCGAGTATTATGGGCTTGAAATTGCAGCTTACGATATCCAGACCAAACGATGTGATTTGTATGGTCAGATGTCTCCATCTGAGGGAGCTCCAGAGGAATTTGATCAGACCATATTTTCTGTGAATCGGGACAGGACAATTGGTCCCATTGAAGGGCAGGCTCTTCATTTTGTTGAAGAACAGCAAAGGAAACGGAGATTCACCGACACGGCCAACTTCACGCTGCGATGTGGGGTTTGCCAAATCGGAGTCATCGGCCAGACGGAGGCTGTAGAACACGCAAAAGCCACTGGCCATGTCAACTTCCAAGAGTATAGATGA

Protein sequence

MKFFNPFHLSINSSSHLTMLRLVIELKHHLVFDKALWFFVEALNAIAFALFLCLLALVGASVGTVIGAFKGHGAAIGAIVGAAAALQLLSCPEPEYRYSLSKEAIMESLMNGKGLMNWASNFSEYMYREMSEFVDVAGESCVEGMSMESVQNLPICEYDHSKMKNSISCPICLEEFEVGEFGRKLPNCDHFFHVDCIDQWLRLNGCCPFCRKFSMPHVHFCATSVAMYIRYVMDHDKHKAAELRQVIAATVASDPTRYCEAFPTVLCAIELSILFEYYGLEIAAYDIQTKRCDLYGQMSPSEGAPEEFDQTIFSVNRDRTIGPIEGQALHFVEEQQRKRRFTDTANFTLRCGVCQIGVIGQTEAVEHAKATGHVNFQEYR

Homology

[Close]

BLAST of CmaCh09G009540.1 vs. ExPASy Swiss-Prot
Match: Q9LPT6 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 2 OS=Arabidopsis thaliana OX=3702 GN=OTU2 PE=1 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 6.1e-51
Identity = 114/188 (60.64%), Postives = 129/188 (68.62%), Query Frame = 0


Query: 229 IRYVMDHDKHKAAELRQVIAATVASDPTRYCEAFPTVL----C-----------AIELSI 288
           I YVMD DK+KA ELRQVIAA VAS+  +Y EAF   L    C           AIELSI
Sbjct: 21  IGYVMDKDKNKAPELRQVIAAAVASNKEKYNEAFLGKLNEEYCAWILNPDKWGGAIELSI 80

Query: 289 LFEYYGLEIAAYDIQTKRCDLYGQ---------------------MSPSEGAPEEFDQTI 348
           L +YYG EIAAYDIQT RCDLYGQ                     +SP EGA E+FD TI
Sbjct: 81  LADYYGREIAAYDIQTSRCDLYGQTRNYDERVMLIYDGLHYDALALSPFEGAEEDFDMTI 140

Query: 349 FSVNRDRTIGPIEGQALHFVEEQQRKRRFTDTANFTLRCGVCQIGVIGQTEAVEHAKATG 381
           + V +DR+IG IEG AL+ V++QQRKR +TDTANFTLRCGVCQIGVIGQ EAVEHA+ATG
Sbjct: 141 YPVGKDRSIGSIEGLALNLVKDQQRKRSYTDTANFTLRCGVCQIGVIGQKEAVEHAQATG 200

[Close]

BLAST of CmaCh09G009540.1 vs. ExPASy Swiss-Prot
Match: Q5F3A6 (Ubiquitin thioesterase OTU1 OS=Gallus gallus OX=9031 GN=YOD1 PE=2 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 7.8e-22
Identity = 77/205 (37.56%), Postives = 96/205 (46.83%), Query Frame = 0


Query: 208 PFCRKFSMPHVHFCATSVAMYIRYVMDHDKHKAAELRQVIAATVASDPTRYCEAF----- 267
           P   +  +P  + C  +   Y+     +D   A E+R +IA  VASDP  YCEA      
Sbjct: 101 PVLARRVVPADNSCLFTSVYYVVEGGVYDPGCAPEMRSLIAQIVASDPEAYCEAVLGKTN 160

Query: 268 ----------PTVLCAIELSILFEYYGLEIAAYDIQTKRCDLYGQ--------------- 327
                      T   AIE+SIL ++Y  EI   D QT R D +G+               
Sbjct: 161 REYCEWIRREETWGGAIEVSILSKFYQCEICVVDTQTVRIDRFGEDAGYTKRVLLIYDGI 220

Query: 328 -MSPSEGAPEEFD---QTIFSVNRDRTIGPIEGQALHFVEEQQRKRRFTDTANFTLRCGV 379
              P E    + D   QTIFS   D  +     QAL   +E +RKR+FTD   FTLRC V
Sbjct: 221 HYDPLERKIPDSDVPPQTIFSTTDDVVL----AQALELADEARRKRQFTDVNRFTLRCMV 280

[Close]

BLAST of CmaCh09G009540.1 vs. ExPASy Swiss-Prot
Match: Q567B1 (Ubiquitin thioesterase OTU1 OS=Danio rerio OX=7955 GN=yod1 PE=2 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 5.0e-21
Identity = 74/190 (38.95%), Postives = 92/190 (48.42%), Query Frame = 0


Query: 229 IRYVMD---HDKHKAAELRQVIAATVASDPTRYCEAF---------------PTVLCAIE 288
           + YVM+   +D   A+E+R +IA  VASDPT Y EA                 T   AIE
Sbjct: 118 VNYVMEGGVYDPACASEMRGLIAQIVASDPTAYSEAVLGKTNEEYCTWIRRDDTWGGAIE 177

Query: 289 LSILFEYYGLEIAAYDIQTKRCDLYGQ----------------------MSPSEGAPEEF 348
           +SIL ++Y  EI   D QT R D +G+                      + P    P   
Sbjct: 178 VSILSKFYQCEICVVDTQTVRVDRFGEDAGYTKRVLLIYDGIHYDPLQKVLPGSDVPA-- 237

Query: 349 DQTIFSVNRDRTIGPIEGQALHFVEEQQRKRRFTDTANFTLRCGVCQIGVIGQTEAVEHA 379
            QT+FS   D     I  QAL   +E +RKR+FTD   F LRC VCQ G++GQ EA EHA
Sbjct: 238 -QTVFSTVDD----VILAQALELADEARRKRQFTDVNRFALRCMVCQTGLVGQKEAREHA 297

[Close]

BLAST of CmaCh09G009540.1 vs. ExPASy Swiss-Prot
Match: Q5VVQ6 (Ubiquitin thioesterase OTU1 OS=Homo sapiens OX=9606 GN=YOD1 PE=1 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 2.8e-19
Identity = 68/173 (39.31%), Postives = 85/173 (49.13%), Query Frame = 0


Query: 240 AAELRQVIAATVASDPTRYCEAF---------------PTVLCAIELSILFEYYGLEIAA 299
           A E+R++IA  VASDP  Y EA                 T   AIE+SIL ++Y  EI  
Sbjct: 179 APEMRRLIAQIVASDPDFYSEAILGKTNQEYCDWIKRDDTWGGAIEISILSKFYQCEICV 238

Query: 300 YDIQTKRCDLYGQ-------------------MSPSEGAPEEFDQTIFSVNRDRTIGPIE 359
            D QT R D +G+                   +  +   P+    TIFS N D  +    
Sbjct: 239 VDTQTVRIDRFGEDAGYTKRVLLIYDGIHYDPLQRNFPDPDTPPLTIFSSNDDIVL---- 298

Query: 360 GQALHFVEEQQRKRRFTDTANFTLRCGVCQIGVIGQTEAVEHAKATGHVNFQE 379
            QAL   +E +R+R+FTD   FTLRC VCQ G+ GQ EA EHAK TGH NF E
Sbjct: 299 VQALELADEARRRRQFTDVNRFTLRCMVCQKGLTGQAEAREHAKETGHTNFGE 347

[Close]

BLAST of CmaCh09G009540.1 vs. ExPASy Swiss-Prot
Match: Q8CB27 (Ubiquitin thioesterase OTU1 OS=Mus musculus OX=10090 GN=Yod1 PE=1 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 4.7e-19
Identity = 66/173 (38.15%), Postives = 85/173 (49.13%), Query Frame = 0


Query: 240 AAELRQVIAATVASDPTRYCEAF---------------PTVLCAIELSILFEYYGLEIAA 299
           A E+R++IA  VASDP  Y EA                 T   AIE+SIL ++Y  EI  
Sbjct: 174 APEMRRLIAQIVASDPVLYSEAILGKTNEDYCDWIRRDDTWGGAIEISILSKFYQCEICV 233

Query: 300 YDIQTKRCDLYGQ-------------------MSPSEGAPEEFDQTIFSVNRDRTIGPIE 359
            D QT R D +G+                   +  +   P+    TIFS N D  +    
Sbjct: 234 VDTQTVRIDRFGEDAGYTKRVLLIYDGIHYDPLQRNFPDPDTPPLTIFSSNDDIVL---- 293

Query: 360 GQALHFVEEQQRKRRFTDTANFTLRCGVCQIGVIGQTEAVEHAKATGHVNFQE 379
            QAL   +E +RKR+FTD   FTLRC +CQ G+ GQ EA +HA+ TGH NF E
Sbjct: 294 VQALELADEARRKRQFTDVNRFTLRCMICQKGLTGQAEARDHARETGHTNFGE 342

[Close]

BLAST of CmaCh09G009540.1 vs. ExPASy TrEMBL
Match: A0A6J1IML2 (NEP1-interacting protein-like 2 OS=Cucurbita maxima OX=3661 GN=LOC111476931 PE=4 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 6.0e-118
Identity = 213/213 (100.00%), Postives = 213/213 (100.00%), Query Frame = 0


Query: 1   MKFFNPFHLSINSSSHLTMLRLVIELKHHLVFDKALWFFVEALNAIAFALFLCLLALVGA 60
           MKFFNPFHLSINSSSHLTMLRLVIELKHHLVFDKALWFFVEALNAIAFALFLCLLALVGA
Sbjct: 1   MKFFNPFHLSINSSSHLTMLRLVIELKHHLVFDKALWFFVEALNAIAFALFLCLLALVGA 60

Query: 61  SVGTVIGAFKGHGAAIGAIVGAAAALQLLSCPEPEYRYSLSKEAIMESLMNGKGLMNWAS 120
           SVGTVIGAFKGHGAAIGAIVGAAAALQLLSCPEPEYRYSLSKEAIMESLMNGKGLMNWAS
Sbjct: 61  SVGTVIGAFKGHGAAIGAIVGAAAALQLLSCPEPEYRYSLSKEAIMESLMNGKGLMNWAS 120

Query: 121 NFSEYMYREMSEFVDVAGESCVEGMSMESVQNLPICEYDHSKMKNSISCPICLEEFEVGE 180
           NFSEYMYREMSEFVDVAGESCVEGMSMESVQNLPICEYDHSKMKNSISCPICLEEFEVGE
Sbjct: 121 NFSEYMYREMSEFVDVAGESCVEGMSMESVQNLPICEYDHSKMKNSISCPICLEEFEVGE 180

Query: 181 FGRKLPNCDHFFHVDCIDQWLRLNGCCPFCRKF 214
           FGRKLPNCDHFFHVDCIDQWLRLNGCCPFCRKF
Sbjct: 181 FGRKLPNCDHFFHVDCIDQWLRLNGCCPFCRKF 213

[Close]

BLAST of CmaCh09G009540.1 vs. ExPASy TrEMBL
Match: A0A6J1F6T6 (NEP1-interacting protein-like 2 OS=Cucurbita moschata OX=3662 GN=LOC111442860 PE=4 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 2.1e-107
Identity = 198/213 (92.96%), Postives = 201/213 (94.37%), Query Frame = 0


Query: 1   MKFFNPFHLSINSSSHLTMLRLVIELKHHLVFDKALWFFVEALNAIAFALFLCLLALVGA 60
           MKFFNPFHLSI SSSHLTMLRLVIELKHH VFDKAL FFV+A N  AFALFLCLLALVGA
Sbjct: 1   MKFFNPFHLSIISSSHLTMLRLVIELKHHPVFDKALLFFVKAFNTTAFALFLCLLALVGA 60

Query: 61  SVGTVIGAFKGHGAAIGAIVGAAAALQLLSCPEPEYRYSLSKEAIMESLMNGKGLMNWAS 120
           SVGTV+GAFKGHG AIGAI GAAAALQLLSCPE EYRYSLSKEAIMESLMNGKGLMNWAS
Sbjct: 61  SVGTVVGAFKGHGVAIGAIAGAAAALQLLSCPESEYRYSLSKEAIMESLMNGKGLMNWAS 120

Query: 121 NFSEYMYREMSEFVDVAGESCVEGMSMESVQNLPICEYDHSKMKNSISCPICLEEFEVGE 180
           NFSEYMYREMSEFVDVAGES VEGMSMESVQNLPICEY HSKMKNSISCPICLEEFE GE
Sbjct: 121 NFSEYMYREMSEFVDVAGESSVEGMSMESVQNLPICEYHHSKMKNSISCPICLEEFEDGE 180

Query: 181 FGRKLPNCDHFFHVDCIDQWLRLNGCCPFCRKF 214
           FGR+LPNCDHFFHVDCIDQWLRLNGCCPFCRKF
Sbjct: 181 FGRELPNCDHFFHVDCIDQWLRLNGCCPFCRKF 213

[Close]

BLAST of CmaCh09G009540.1 vs. ExPASy TrEMBL
Match: A0A0A0K5D1 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G209550 PE=4 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 5.7e-76
Identity = 154/213 (72.30%), Postives = 168/213 (78.87%), Query Frame = 0


Query: 1   MKFFNPFHLSINSSSHLTMLRLVIELKHHLVFDKALWFFVEALNAIAFALFLCLLALVGA 60
           M+FF  F + + S S  TM  LV+E K  L F K L F  EAL  I FALFL LLALVGA
Sbjct: 1   MQFFKLFDMFLISCSEFTM--LVLEFKEQLFFHKPLRFLTEALKMITFALFLSLLALVGA 60

Query: 61  SVGTVIGAFKGHGAAIGAIVGAAAALQLLSCPEPEYRYSLSKEAIMESLMNGKGLMNWAS 120
           SVGTVIGAFKGHG  IGAI GAA ALQ+ S     +RY LS+EAIME LM+GKGLMNWAS
Sbjct: 61  SVGTVIGAFKGHGTGIGAIAGAAVALQIFSSSASGHRYCLSREAIMERLMDGKGLMNWAS 120

Query: 121 NFSEYMYREMSEFVDVAGESCVEGMSMESVQNLPICEYDHSKMKNSISCPICLEEFEVGE 180
           NFSEYMYRE+SEFVDVAGESCVEG+SMESVQ LP+CEYDH+ + NSISC ICLEEFE GE
Sbjct: 121 NFSEYMYREISEFVDVAGESCVEGLSMESVQMLPVCEYDHTTV-NSISCSICLEEFEDGE 180

Query: 181 FGRKLPNCDHFFHVDCIDQWLRLNGCCPFCRKF 214
            GR LPNCDHFFHV CIDQWL L+G CPFCRKF
Sbjct: 181 IGRILPNCDHFFHVVCIDQWLHLHGSCPFCRKF 210

[Close]

BLAST of CmaCh09G009540.1 vs. ExPASy TrEMBL
Match: A0A1S3BIQ9 (NEP1-interacting protein-like 2 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490143 PE=4 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 1.5e-73
Identity = 148/193 (76.68%), Postives = 159/193 (82.38%), Query Frame = 0


Query: 22  LVIELKHHLVFDKALWFFVEALNAIAFALFLCLLALVGASVGTVIGAFKGHGAAIGAIVG 81
           LV+E K  L FDKAL F  EAL  IA AL L LLALVGA VGTVIGAFKGHGAAIGAI G
Sbjct: 2   LVVEFKEQLFFDKALRFLAEALKTIALALLLSLLALVGACVGTVIGAFKGHGAAIGAIAG 61

Query: 82  AAAALQLLSCPEPEYR-YSLSKEAIMESLMNGKGLMNWASNFSEYMYREMSEFVDVAGES 141
           AA ALQ++SC     R Y LS+EAIMESLM+GKGLMNWASNFSEYM+RE+SEFVDVAGES
Sbjct: 62  AAVALQIVSCTASGQRYYCLSREAIMESLMDGKGLMNWASNFSEYMHREISEFVDVAGES 121

Query: 142 CVEGMSMESVQNLPICEYDHSKMKNSISCPICLEEFEVGEFGRKLPNCDHFFHVDCIDQW 201
           C EG+SMESVQ LP+CEYDHS + NSISC ICLEEFE GE GR LPNCDHFFHV CIDQW
Sbjct: 122 CAEGLSMESVQMLPVCEYDHSTI-NSISCSICLEEFEDGEIGRILPNCDHFFHVGCIDQW 181

Query: 202 LRLNGCCPFCRKF 214
           L L+G CP CRKF
Sbjct: 182 LHLHGSCPICRKF 193

[Close]

BLAST of CmaCh09G009540.1 vs. ExPASy TrEMBL
Match: A0A1S3BI36 (NEP1-interacting protein-like 2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490143 PE=4 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 2.9e-72
Identity = 147/195 (75.38%), Postives = 158/195 (81.03%), Query Frame = 0


Query: 22  LVIELKHHLVFDKALWFFVEALNAIAFALFLCLLALVGASVGTVIGAFKGHGAAIGAIVG 81
           LV+E K  L FDKAL F  EAL  IA AL L LLALVGA VGTVIGAFKGHGAAIGAI G
Sbjct: 2   LVVEFKEQLFFDKALRFLAEALKTIALALLLSLLALVGACVGTVIGAFKGHGAAIGAIAG 61

Query: 82  AAAALQLLSCPEPEYRY---SLSKEAIMESLMNGKGLMNWASNFSEYMYREMSEFVDVAG 141
           AA ALQ++SC     RY   S  +EAIMESLM+GKGLMNWASNFSEYM+RE+SEFVDVAG
Sbjct: 62  AAVALQIVSCTASGQRYYCLSRWQEAIMESLMDGKGLMNWASNFSEYMHREISEFVDVAG 121

Query: 142 ESCVEGMSMESVQNLPICEYDHSKMKNSISCPICLEEFEVGEFGRKLPNCDHFFHVDCID 201
           ESC EG+SMESVQ LP+CEYDHS + NSISC ICLEEFE GE GR LPNCDHFFHV CID
Sbjct: 122 ESCAEGLSMESVQMLPVCEYDHSTI-NSISCSICLEEFEDGEIGRILPNCDHFFHVGCID 181

Query: 202 QWLRLNGCCPFCRKF 214
           QWL L+G CP CRKF
Sbjct: 182 QWLHLHGSCPICRKF 195

[Close]

BLAST of CmaCh09G009540.1 vs. NCBI nr
Match: XP_022976578.1 (NEP1-interacting protein-like 2 [Cucurbita maxima])

HSP 1 Score: 434.1 bits (1115), Expect = 1.2e-117
Identity = 213/213 (100.00%), Postives = 213/213 (100.00%), Query Frame = 0


Query: 1   MKFFNPFHLSINSSSHLTMLRLVIELKHHLVFDKALWFFVEALNAIAFALFLCLLALVGA 60
           MKFFNPFHLSINSSSHLTMLRLVIELKHHLVFDKALWFFVEALNAIAFALFLCLLALVGA
Sbjct: 1   MKFFNPFHLSINSSSHLTMLRLVIELKHHLVFDKALWFFVEALNAIAFALFLCLLALVGA 60

Query: 61  SVGTVIGAFKGHGAAIGAIVGAAAALQLLSCPEPEYRYSLSKEAIMESLMNGKGLMNWAS 120
           SVGTVIGAFKGHGAAIGAIVGAAAALQLLSCPEPEYRYSLSKEAIMESLMNGKGLMNWAS
Sbjct: 61  SVGTVIGAFKGHGAAIGAIVGAAAALQLLSCPEPEYRYSLSKEAIMESLMNGKGLMNWAS 120

Query: 121 NFSEYMYREMSEFVDVAGESCVEGMSMESVQNLPICEYDHSKMKNSISCPICLEEFEVGE 180
           NFSEYMYREMSEFVDVAGESCVEGMSMESVQNLPICEYDHSKMKNSISCPICLEEFEVGE
Sbjct: 121 NFSEYMYREMSEFVDVAGESCVEGMSMESVQNLPICEYDHSKMKNSISCPICLEEFEVGE 180

Query: 181 FGRKLPNCDHFFHVDCIDQWLRLNGCCPFCRKF 214
           FGRKLPNCDHFFHVDCIDQWLRLNGCCPFCRKF
Sbjct: 181 FGRKLPNCDHFFHVDCIDQWLRLNGCCPFCRKF 213

[Close]

BLAST of CmaCh09G009540.1 vs. NCBI nr
Match: XP_022936186.1 (NEP1-interacting protein-like 2 [Cucurbita moschata])

HSP 1 Score: 399.1 bits (1024), Expect = 4.4e-107
Identity = 198/213 (92.96%), Postives = 201/213 (94.37%), Query Frame = 0


Query: 1   MKFFNPFHLSINSSSHLTMLRLVIELKHHLVFDKALWFFVEALNAIAFALFLCLLALVGA 60
           MKFFNPFHLSI SSSHLTMLRLVIELKHH VFDKAL FFV+A N  AFALFLCLLALVGA
Sbjct: 1   MKFFNPFHLSIISSSHLTMLRLVIELKHHPVFDKALLFFVKAFNTTAFALFLCLLALVGA 60

Query: 61  SVGTVIGAFKGHGAAIGAIVGAAAALQLLSCPEPEYRYSLSKEAIMESLMNGKGLMNWAS 120
           SVGTV+GAFKGHG AIGAI GAAAALQLLSCPE EYRYSLSKEAIMESLMNGKGLMNWAS
Sbjct: 61  SVGTVVGAFKGHGVAIGAIAGAAAALQLLSCPESEYRYSLSKEAIMESLMNGKGLMNWAS 120

Query: 121 NFSEYMYREMSEFVDVAGESCVEGMSMESVQNLPICEYDHSKMKNSISCPICLEEFEVGE 180
           NFSEYMYREMSEFVDVAGES VEGMSMESVQNLPICEY HSKMKNSISCPICLEEFE GE
Sbjct: 121 NFSEYMYREMSEFVDVAGESSVEGMSMESVQNLPICEYHHSKMKNSISCPICLEEFEDGE 180

Query: 181 FGRKLPNCDHFFHVDCIDQWLRLNGCCPFCRKF 214
           FGR+LPNCDHFFHVDCIDQWLRLNGCCPFCRKF
Sbjct: 181 FGRELPNCDHFFHVDCIDQWLRLNGCCPFCRKF 213

[Close]

BLAST of CmaCh09G009540.1 vs. NCBI nr
Match: XP_023536459.1 (NEP1-interacting protein-like 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 397.9 bits (1021), Expect = 9.8e-107
Identity = 197/213 (92.49%), Postives = 200/213 (93.90%), Query Frame = 0


Query: 1   MKFFNPFHLSINSSSHLTMLRLVIELKHHLVFDKALWFFVEALNAIAFALFLCLLALVGA 60
           MKFFNPFHLSI SSSHLTMLRLVIELKHH +FDKAL FFVEAL    FALFLCLLALVGA
Sbjct: 1   MKFFNPFHLSIISSSHLTMLRLVIELKHHPIFDKALRFFVEALKTTTFALFLCLLALVGA 60

Query: 61  SVGTVIGAFKGHGAAIGAIVGAAAALQLLSCPEPEYRYSLSKEAIMESLMNGKGLMNWAS 120
           SVGTVIGAFKGHG AIGAI GAAAALQLLS PE EYRYSLSKEAIMESLMNGKGLMNWAS
Sbjct: 61  SVGTVIGAFKGHGVAIGAIAGAAAALQLLSYPESEYRYSLSKEAIMESLMNGKGLMNWAS 120

Query: 121 NFSEYMYREMSEFVDVAGESCVEGMSMESVQNLPICEYDHSKMKNSISCPICLEEFEVGE 180
           NFSEYMYREMSEFVDVAGESCVEGMSMESVQNLPIC+YDH KMKNSISCPICLEEFE GE
Sbjct: 121 NFSEYMYREMSEFVDVAGESCVEGMSMESVQNLPICDYDHGKMKNSISCPICLEEFEDGE 180

Query: 181 FGRKLPNCDHFFHVDCIDQWLRLNGCCPFCRKF 214
           FGR+LPNCDHFFHVDCIDQWLRLNGCCPFCRKF
Sbjct: 181 FGRRLPNCDHFFHVDCIDQWLRLNGCCPFCRKF 213

[Close]

BLAST of CmaCh09G009540.1 vs. NCBI nr
Match: KAG6607800.1 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 368.2 bits (944), Expect = 8.3e-98
Identity = 227/399 (56.89%), Postives = 247/399 (61.90%), Query Frame = 0


Query: 19  MLRLVIELKHHLVFDKALWFFVEALNAIAFALFLCLLALVGASVGTVIGAFKGHGAAIGA 78
           M+  ++ELK   VFD  L FFV++   I FALFLCLLALVGA VGTV+GAFKGH  +I  
Sbjct: 1   MVWWLVELKEE-VFDTTLRFFVDSFKTILFALFLCLLALVGAMVGTVVGAFKGHSTSIST 60

Query: 79  IVGAAAALQLLSCPEPEYRYSLSKEAIMESLMNGKGLMNWASNFSEYMYREMSEFVD-VA 138
           IVGA  ALQLL      YRYS                   AS+  E+M+RE+SEFVD  A
Sbjct: 61  IVGAIVALQLLEYTTFAYRYSFPN----------------ASDLLEHMHREISEFVDATA 120

Query: 139 GESCVEGMSMESVQNLPICEYDHSKMKNSISCPICLEEFEVGEFGRKLPNCDHFFHVDCI 198
           GESCVEGM MESVQNL + EYDH KM +SISC ICLE               H      +
Sbjct: 121 GESCVEGMPMESVQNLAVSEYDHRKMNSSISCSICLEVI-----------FTHTMEGTVL 180

Query: 199 DQWLRLNGCCPFCRKFSMPHVHFCATSVAMYIRYVMDHDKHKAAELRQVIAATVASDPTR 258
            + +  +  C F                   + YVMDHDK KA ELRQVIAATVASDPTR
Sbjct: 181 RRVIPSDNSCLF-----------------NAVGYVMDHDKDKATELRQVIAATVASDPTR 240

Query: 259 YCEAF----PTVLC-----------AIELSILFEYYGLEIAAYDIQTKRCDLYGQ----- 318
           Y EAF        C           AIELSIL EYYG EIAAYDIQT RCDLYGQ     
Sbjct: 241 YSEAFLGKPNEEYCAWILDSEKWGGAIELSILSEYYGREIAAYDIQTTRCDLYGQEKKYS 300

Query: 319 ----------------MSPSEGAPEEFDQTIFSVNRDRTIGPIEGQALHFVEEQQRKRRF 378
                           MSP EGAPEEFDQTIFSVNRDR+IGPIE QALHFV+EQQRKR F
Sbjct: 301 ERVLLIYDGLHYDALVMSPFEGAPEEFDQTIFSVNRDRSIGPIEEQALHFVKEQQRKRSF 354

Query: 379 TDTANFTLRCGVCQIGVIGQTEAVEHAKATGHVNFQEYR 381
           TD ANFTLRCGVCQIGVIGQTEAVEHAKATGHVNFQEYR
Sbjct: 361 TDAANFTLRCGVCQIGVIGQTEAVEHAKATGHVNFQEYR 354

[Close]

BLAST of CmaCh09G009540.1 vs. NCBI nr
Match: KAG7024921.1 (NEP1-interacting protein-like 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 364.0 bits (933), Expect = 1.6e-96
Identity = 180/195 (92.31%), Postives = 184/195 (94.36%), Query Frame = 0


Query: 19  MLRLVIELKHHLVFDKALWFFVEALNAIAFALFLCLLALVGASVGTVIGAFKGHGAAIGA 78
           MLRLVIELKHH VFDKAL FFV+A N  AFALFLCLLALVGASVGTV+GAFKGHG AIGA
Sbjct: 1   MLRLVIELKHHPVFDKALRFFVKAFNTTAFALFLCLLALVGASVGTVVGAFKGHGVAIGA 60

Query: 79  IVGAAAALQLLSCPEPEYRYSLSKEAIMESLMNGKGLMNWASNFSEYMYREMSEFVDVAG 138
           I GAAAALQLLSCPE EYRYSLSKEAIMESLMNGKGLMNWA+NFSEYMYREMSEFVDVAG
Sbjct: 61  IAGAAAALQLLSCPESEYRYSLSKEAIMESLMNGKGLMNWATNFSEYMYREMSEFVDVAG 120

Query: 139 ESCVEGMSMESVQNLPICEYDHSKMKNSISCPICLEEFEVGEFGRKLPNCDHFFHVDCID 198
           ES VEGMSMESVQNLPICEY HSKMKNSISCPICLEEFE GEFGR+LPNCDHFFHVDCID
Sbjct: 121 ESRVEGMSMESVQNLPICEYHHSKMKNSISCPICLEEFEDGEFGRELPNCDHFFHVDCID 180

Query: 199 QWLRLNGCCPFCRKF 214
           QWLRLNGCCPFCRKF
Sbjct: 181 QWLRLNGCCPFCRKF 195

[Close]

BLAST of CmaCh09G009540.1 vs. TAIR 10
Match: AT1G50670.1 (OTU-like cysteine protease family protein )

HSP 1 Score: 203.0 bits (515), Expect = 4.3e-52
Identity = 114/188 (60.64%), Postives = 129/188 (68.62%), Query Frame = 0


Query: 229 IRYVMDHDKHKAAELRQVIAATVASDPTRYCEAFPTVL----C-----------AIELSI 288
           I YVMD DK+KA ELRQVIAA VAS+  +Y EAF   L    C           AIELSI
Sbjct: 21  IGYVMDKDKNKAPELRQVIAAAVASNKEKYNEAFLGKLNEEYCAWILNPDKWGGAIELSI 80

Query: 289 LFEYYGLEIAAYDIQTKRCDLYGQ---------------------MSPSEGAPEEFDQTI 348
           L +YYG EIAAYDIQT RCDLYGQ                     +SP EGA E+FD TI
Sbjct: 81  LADYYGREIAAYDIQTSRCDLYGQTRNYDERVMLIYDGLHYDALALSPFEGAEEDFDMTI 140

Query: 349 FSVNRDRTIGPIEGQALHFVEEQQRKRRFTDTANFTLRCGVCQIGVIGQTEAVEHAKATG 381
           + V +DR+IG IEG AL+ V++QQRKR +TDTANFTLRCGVCQIGVIGQ EAVEHA+ATG
Sbjct: 141 YPVGKDRSIGSIEGLALNLVKDQQRKRSYTDTANFTLRCGVCQIGVIGQKEAVEHAQATG 200

[Close]

BLAST of CmaCh09G009540.1 vs. TAIR 10
Match: AT3G20395.1 (RING/U-box superfamily protein )

HSP 1 Score: 103.6 bits (257), Expect = 3.6e-22
Identity = 62/180 (34.44%), Postives = 91/180 (50.56%), Query Frame = 0


Query: 51  FLCLLALVGASVGTVIGAFKGH--------GAAIGAIVGAAAALQLLSCPEPEYRYSLSK 110
           F+ +LA     V  ++GA +GH        G+ +G + G   A+QL   P       LSK
Sbjct: 38  FIFILASASVVVAAIVGAIEGHTTDIGFLQGSVLGVVAGVITAVQLFG-PVLHSDQPLSK 97

Query: 111 EAIMESLMNGKGLM-----------NWASNFSEYMYREMSEFVDVAGESCVEGMSMESVQ 170
            A++  ++NGK +M            W     +  Y E S   D   E   +G+S  S+Q
Sbjct: 98  VALLRRVVNGKAIMGLVRPFVLKAYQWQIIALDTSYMESSNLYDFNHEK--KGLSKSSIQ 157

Query: 171 NLPICEYDHSKMKNSISCPICLEEFEVGEFGRKLPNCDHFFHVDCIDQWLRLNGCCPFCR 212
           N+P+  Y+ S+ +   SC ICL+++E GE GRKL  C H FH++CID+WL     CP CR
Sbjct: 158 NIPMF-YNRSEHQTKSSCSICLQDWEEGEVGRKLARCGHTFHMNCIDEWLLRQETCPICR 213

[Close]

BLAST of CmaCh09G009540.1 vs. TAIR 10
Match: AT1G74410.1 (RING/U-box superfamily protein )

HSP 1 Score: 92.0 bits (227), Expect = 1.1e-18
Identity = 58/189 (30.69%), Postives = 89/189 (47.09%), Query Frame = 0


Query: 54  LLALVGASVGTVIGAFKG--------HGAAIGAIVGAAAALQLLSCP------------- 113
           + A+ GA  G V GA  G         GAA+GA+ GA  ++++L                
Sbjct: 37  IFAMAGAFTGAVTGAVAGRAAQYGVLRGAALGAVAGAILSVEVLEASRAYWYLELSGSRG 96

Query: 114 --------EPEYRYSLSKEAIMESLMNGKGLMNWASNFSEYMYREMSEFVDVAGESCVEG 173
                   E  +R  L  E +M +++N     +W    S+  Y E     DV GE    G
Sbjct: 97  PSSMADFVEQLFRGRLVDEQLMSTMINSH---HWQLRISDVSYEERE---DVYGELEARG 156

Query: 174 MSMESVQNLPICEYDHSKMKNSIS-CPICLEEFEVGEFGRKLPNCDHFFHVDCIDQWLRL 213
           +S +S++ LP        ++  ++ C ICL++ + GE  R LP CDH FH+ C+D+WL  
Sbjct: 157 LSGDSLRKLPCYIMSSEMVRRQVTHCTICLQDIKTGEITRSLPKCDHTFHLVCVDKWLIR 216

[Close]

BLAST of CmaCh09G009540.1 vs. TAIR 10
Match: AT5G66070.1 (RING/U-box superfamily protein )

HSP 1 Score: 84.7 bits (208), Expect = 1.7e-16
Identity = 61/199 (30.65%), Postives = 97/199 (48.74%), Query Frame = 0


Query: 38  FFVEALNAIAF-ALFLCLLALVGASVGTVIGAFKG--------HGAAIGAIVGAA----- 97
           F V AL A  F ALF    ALVG  +G + GA  G         GAA+GAI GA      
Sbjct: 22  FAVSALLANLFSALFTFFFALVGTLLGALTGALIGQETESGFIRGAAVGAISGAVFSIEV 81

Query: 98  --AALQLLSCPEPEYRYSLSKEAIMESLMNGK--------GLMNWASNFSEYMYREMSEF 157
             ++L L    E      L    ++ SL++G+         +++   +    +  +  + 
Sbjct: 82  FESSLLLWQSDESGIGCLLYLIDVIASLLSGRLVRERIGPAMLSAVQSQMGAVESQFQDH 141

Query: 158 VDVAGESCVEGMSMESVQNLPICEYDHSKMKNSISCPICLEEFEVGEFGRKLPNCDHFFH 213
            D+   +  +G++ +S+  +P      +     +SC +CL++F+VGE  R LP+C H FH
Sbjct: 142 TDIFDTAISKGLTGDSLNRIPKVRITDTS-PEIVSCSVCLQDFQVGETVRSLPHCHHMFH 201

[Close]

BLAST of CmaCh09G009540.1 vs. TAIR 10
Match: AT2G17730.1 (NEP-interacting protein 2 )

HSP 1 Score: 80.1 bits (196), Expect = 4.2e-15
Identity = 61/206 (29.61%), Postives = 95/206 (46.12%), Query Frame = 0


Query: 35  ALWFFVEA-LNAIAFALFLCLLALVGASVGTVIGAFKG--------HGAAIGAIVGAA-- 94
           A  F V A L  I  A+     ALVG  +G + GA  G         GAAIGAI GA   
Sbjct: 34  ACHFLVSAVLGTIISAILTFFFALVGTLLGALTGALIGQETESGFIRGAAIGAISGAVFS 93

Query: 95  -----AALQLLSCPEPEYRYSLSKEAIMESLMNGK-----------GLMNWASNFSEYMY 154
                ++L L    E  +   L    ++ SL++G+             +       +  +
Sbjct: 94  IEVFESSLDLWKSDESGFGCFLYLIDVIVSLLSGRLVRERIGPAMLSAVQSQMGAVDTAF 153

Query: 155 REMSEFVDVAGESCVEGMSMESVQNLPICEYDHS-KMKNSISCPICLEEFEVGEFGRKLP 213
            + +   D  G   + G  +E +  + I   +++   +N+ SC +CL++F++GE  R LP
Sbjct: 154 DDHTSLFDTGGSKGLTGDLVEKIPKMTITGNNNTDASENTDSCSVCLQDFQLGETVRSLP 213

The following BLAST results are available for this feature:

BLAST of CmaCh09G009540.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LPT6	6.1e-51	60.64	OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 2 OS=Arabidopsis thalian...	[more]
Q5F3A6	7.8e-22	37.56	Ubiquitin thioesterase OTU1 OS=Gallus gallus OX=9031 GN=YOD1 PE=2 SV=1	[more]
Q567B1	5.0e-21	38.95	Ubiquitin thioesterase OTU1 OS=Danio rerio OX=7955 GN=yod1 PE=2 SV=1	[more]
Q5VVQ6	2.8e-19	39.31	Ubiquitin thioesterase OTU1 OS=Homo sapiens OX=9606 GN=YOD1 PE=1 SV=1	[more]
Q8CB27	4.7e-19	38.15	Ubiquitin thioesterase OTU1 OS=Mus musculus OX=10090 GN=Yod1 PE=1 SV=1	[more]

BLAST of CmaCh09G009540.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IML2	6.0e-118	100.00	NEP1-interacting protein-like 2 OS=Cucurbita maxima OX=3661 GN=LOC111476931 PE=4...	[more]
A0A6J1F6T6	2.1e-107	92.96	NEP1-interacting protein-like 2 OS=Cucurbita moschata OX=3662 GN=LOC111442860 PE...	[more]
A0A0A0K5D1	5.7e-76	72.30	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G209550 P...	[more]
A0A1S3BIQ9	1.5e-73	76.68	NEP1-interacting protein-like 2 isoform X2 OS=Cucumis melo OX=3656 GN=LOC1034901...	[more]
A0A1S3BI36	2.9e-72	75.38	NEP1-interacting protein-like 2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1034901...	[more]

BLAST of CmaCh09G009540.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022976578.1	1.2e-117	100.00	NEP1-interacting protein-like 2 [Cucurbita maxima]	[more]
XP_022936186.1	4.4e-107	92.96	NEP1-interacting protein-like 2 [Cucurbita moschata]	[more]
XP_023536459.1	9.8e-107	92.49	NEP1-interacting protein-like 2 [Cucurbita pepo subsp. pepo]	[more]
KAG6607800.1	8.3e-98	56.89	OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 2, partial [Cucurbita ar...	[more]
KAG7024921.1	1.6e-96	92.31	NEP1-interacting protein-like 2, partial [Cucurbita argyrosperma subsp. argyrosp...	[more]

BLAST of CmaCh09G009540.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G50670.1	4.3e-52	60.64	OTU-like cysteine protease family protein	[more]
AT3G20395.1	3.6e-22	34.44	RING/U-box superfamily protein	[more]
AT1G74410.1	1.1e-18	30.69	RING/U-box superfamily protein	[more]
AT5G66070.1	1.7e-16	30.65	RING/U-box superfamily protein	[more]
AT2G17730.1	4.2e-15	29.61	NEP-interacting protein 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 169..210 e-value: 3.1E-5 score: 33.4
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 168..211 e-value: 2.8E-12 score: 46.8
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 169..211 score: 12.061441
None	No IPR available	GENE3D	3.90.70.80		coord: 264..345 e-value: 2.0E-6 score: 29.7
None	No IPR available	PANTHER	PTHR46151:SF12	RING/U-BOX SUPERFAMILY PROTEIN	coord: 25..212
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 150..217
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 135..215 e-value: 2.7E-18 score: 67.4
IPR044523	RING domain-containing protein NIP1/2-like	PANTHER	PTHR46151	NEP1-INTERACTING PROTEIN-LIKE 2	coord: 25..212

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmaCh09G009540	CmaCh09G009540	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh09G009540.1:exon:954	CmaCh09G009540.1:exon:954	exon
CmaCh09G009540.1:exon:953	CmaCh09G009540.1:exon:953	exon
CmaCh09G009540.1:exon:952	CmaCh09G009540.1:exon:952	exon
CmaCh09G009540.1:exon:951	CmaCh09G009540.1:exon:951	exon
CmaCh09G009540.1:exon:950	CmaCh09G009540.1:exon:950	exon
CmaCh09G009540.1:exon:949	CmaCh09G009540.1:exon:949	exon
CmaCh09G009540.1:exon:948	CmaCh09G009540.1:exon:948	exon
CmaCh09G009540.1:exon:947	CmaCh09G009540.1:exon:947	exon
CmaCh09G009540.1:exon:946	CmaCh09G009540.1:exon:946	exon
CmaCh09G009540.1:exon:945	CmaCh09G009540.1:exon:945	exon
CmaCh09G009540.1:exon:944	CmaCh09G009540.1:exon:944	exon

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh09G009540.1:three_prime_utr	CmaCh09G009540.1:three_prime_utr	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh09G009540.1:cds	CmaCh09G009540.1:cds_11	CDS
CmaCh09G009540.1:cds	CmaCh09G009540.1:cds_10	CDS
CmaCh09G009540.1:cds	CmaCh09G009540.1:cds_9	CDS
CmaCh09G009540.1:cds	CmaCh09G009540.1:cds_8	CDS
CmaCh09G009540.1:cds	CmaCh09G009540.1:cds_7	CDS
CmaCh09G009540.1:cds	CmaCh09G009540.1:cds_6	CDS
CmaCh09G009540.1:cds	CmaCh09G009540.1:cds_5	CDS
CmaCh09G009540.1:cds	CmaCh09G009540.1:cds_4	CDS
CmaCh09G009540.1:cds	CmaCh09G009540.1:cds_3	CDS
CmaCh09G009540.1:cds	CmaCh09G009540.1:cds_2	CDS
CmaCh09G009540.1:cds	CmaCh09G009540.1:cds	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh09G009540.1:five_prime_utr	CmaCh09G009540.1:five_prime_utr	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmaCh09G009540.1	CmaCh09G009540.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0044267	cellular protein metabolic process
biological_process	GO:0006508	proteolysis
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0101005	deubiquitinase activity