CmaCh06G004150.1 (mRNA) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh06G004150.1
Type	mRNA
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	caffeic acid 3-O-methyltransferase
Location	Cma_Chr06: 1924548 .. 1927568 (-)
Sequence length	1621
RNA-Seq Expression	CmaCh06G004150.1
Synteny	CmaCh06G004150.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAACGCGGCTAAGTGTACACCTCTAGTTAAAAACTTGATAGCTAGGTTTTTGTTCTCTTCCCAAAACGTACGTGTGGTCCTTATAGCTCACCAATCTTCCACATCCTGATAAAGCCTAGTACCGTCGTCGTCAATCTCAAAATCAAAATGGTCGGAAAGGAAGATCCGCCGATGGGGAAAGAATCCGCCAACACCGTTGCCGGAAGCGAGAATCATAAAACAGCGAGGCTAGCCATACTAGAGCTCGCAAACATGATCAGCGTTCCGATGTCCCTCAATGCCGTCGTCCGTCTCAAAATCGCCGACGCTATCTGGCAAGGAGGTTCCAATTTTCCGCTCTCTGCTTCTGAGATCCTCGCACGCTTGGTTCCATCCGGTGGCGACGCCGATAATCTCGAGCGGATCCTCCGTATGCTCACTAGCTATGGCGTTTTCGAAGAGCATCTCAACCCTAACTCGTCGGACCGCCGGTATTCCCTCACCGACGTAGGAAAAACTCTGGTCACCGATTCCGATGGTCTCTCTTACGCGTCGTACGTGCTTCAGCATCATCAGGTCCGTGATTATTGCGTATGTCAGATTTCTGTTACAACAGATTAGTTTCGATCCTTTGGCCGTTCTGATTCTGAATTTCTAATTTCTGTGCCGTGTACAGGACGCGCTTATGAGAGCGTGGCCAAGAGTGCACGAGGCTGCGATTGACGCTACTACGGAGCCGTTTGTCAGAGCTAATGGCGAAGCAGCGTACAGTTACTACGGGAAGAATGAGGAGATGAACTTGTTAATGCAGAGAGCGATGGCCGGCGTATCAGTGCCGTTCATGAAGGCTGTATTGGACGGCTACGATGGCTTCGGAGGCGTGGAGAAATTGGTCGACGTCGGCGGCAGCGCCGGTGATTGCTTGAGGATGATCCTACAGAAACATCCTCACATTAAGGAAGGAATCAACTTCGATCTGCCGGAAGTGGTGGCTAGGGCTCCCAGCATCCCCGGTGAGCTTTTTTGTGATTAATTAACGTCTATAGCTGTGATTATGCAGCTTTCATTTTTAATTAATTAACCTGCTTAATTGTAATTATCATTACTAAATTAATTAACGTATTTTAATTATTTATTATTCGTGGTTTAAATTTAAAAAAAAAAATAGCAATTTTTCTTTTAATGAGGATGTATGTAGCGAAAAAGTGAGTAGCTGTATGCTAAACTGACAGGGGTGACTCACGTTGGAGGCGACATGTTCAAGTCCGTCCCCACCGGTGACGCTATTTTCATGAAGGTACGTGTAGCTTTTGCCTTTTTATTTTCTGTAAAAACCCTACGGATTTTAATTTCCATTTCTCGCACGTGACACTGGGCCCTCCCAGTTTCAGCTACCTACTTGAACAATAAATAATTTTGCCTCTTAAAAAATACAAATTAGTATTTATATAATTGTTACAAAATAAAATTTAAAGACTGAATTATTAAAGTTAGGGATTAAATTTCATAAAAGTTTAAGAGCCAAAAATATTTGTCAACAAAAAAAAATGTTTTTAAACCTACTATAAATGAAAATAATATTTATATAATTGAAAAATAACTATAAAAATCCAATATTGATAGAAGAAAATATATATTCCAATTTGTCAATTTAATTGAGTAACATAATAATATTAAAAAATTTGTAGGTTAAAATATTAGTTCAGTTGCCTCATTTTAAGTTCATGTGTGAAATTTAAAATGATATATTATTATTTTATTATTAACCTTTTAATTTATTTTCTATAAATTTTAAAAATATGTTTTCTTGATGTTTTTTTTTTTTTGCATGATTATTAATATCATTTAATCAATTTTGATAAAAATGACGGATTAAATTTAAGATTTATGAAAAAAAATATATGAAAACTAAATGAAAATGCAAATACCAAATTTGTATTATTCAAAGCAAAATACGTATGGACTTTGTCGGTAGGAGTTTTTTTTTTTTTTTTTAGAAATAAAAAATATTTTTAAAAAAATGGATAAGGAAGGAGAAGAAAATGAAAGGTGTAAACTGACATATCTATGTTTTCAAAAACAAAATAAAACAAATAAATATTTTCTTAAAAGAAATGAAATACTACATTTTTATTAAATGATGATTTTATTTTATATAGATTATGTTCAAATGTAAATACTGCATTTTTATTAATAAAAGTTGATTTGAAATACATGTTTTCTTCCAAATTCAAGGTGAAGATATTATTTAGATTATTATTAAAAAAAAAAAAAAAAAATCTCAATTTCAACCCAAAGGGTAATTAGGCATTTTAGAAATGTAGCAACTAAAATAGATAAAAAGTTAGGAAGGCATTCTGAAATTGGATTGTGGGCAGTGGGTTCTGTCAACATGGACGGATGATGAATGCAAGATGATATTGGAGAACTGCTGCAAGTCACTCCCGGCGGGAGGGAAACTGATCGCCTGCGAGCCGACGGTGCCTGAGAAGACGGACGACAGTCACCGGACACGTGCCCTTTTAGCCAACGACATATTCATCATGACCATTTACAGTGCGAAGAGTAAGCAGAGGACTGAGGATGAGTTCCGGCAGCTGGGCCTCTCCGTCGGCTTCTCCGGCATGCGCTCATTCCACGTGGATTACTTCTATTGTGTGTTGGAATTCCAGAAGTGAAGGCTTGGATGTTATTGCAGAGATTTTAGATTCGGCTGTTTACAATTGGCATTGGAATCTGAACAAATATTTTGATTAGTATTCAAACAATGGTGTAGTGGAAGATTGGTTGGGTTGGGTGGAGCAACCTAACACCCCTCACACACTCTGTGATTTTCTGTCTTCTTCCTCATTGTGGGTTCCAGCCATGGCCCTCCCCTTCCCGCCAGTATTCACCCATCCCCATTCTAATCATATCTTACCCACATTTCCATTTTTATTTCTGATTTTTTGTCATATTAATATCCAAATGAGTTTTTTTTTTTTTTTTTACTTGTTATTTTTTACCGTCTTATTTATATCGAGGTGAGCTGTTTTAACCGTG

mRNA sequence

CAACGCGGCTAAGTGTACACCTCTAGTTAAAAACTTGATAGCTAGGTTTTTGTTCTCTTCCCAAAACGTACGTGTGGTCCTTATAGCTCACCAATCTTCCACATCCTGATAAAGCCTAGTACCGTCGTCGTCAATCTCAAAATCAAAATGGTCGGAAAGGAAGATCCGCCGATGGGGAAAGAATCCGCCAACACCGTTGCCGGAAGCGAGAATCATAAAACAGCGAGGCTAGCCATACTAGAGCTCGCAAACATGATCAGCGTTCCGATGTCCCTCAATGCCGTCGTCCGTCTCAAAATCGCCGACGCTATCTGGCAAGGAGGTTCCAATTTTCCGCTCTCTGCTTCTGAGATCCTCGCACGCTTGGTTCCATCCGGTGGCGACGCCGATAATCTCGAGCGGATCCTCCGTATGCTCACTAGCTATGGCGTTTTCGAAGAGCATCTCAACCCTAACTCGTCGGACCGCCGGTATTCCCTCACCGACGTAGGAAAAACTCTGGTCACCGATTCCGATGGTCTCTCTTACGCGTCGTACGTGCTTCAGCATCATCAGGACGCGCTTATGAGAGCGTGGCCAAGAGTGCACGAGGCTGCGATTGACGCTACTACGGAGCCGTTTGTCAGAGCTAATGGCGAAGCAGCGTACAGTTACTACGGGAAGAATGAGGAGATGAACTTGTTAATGCAGAGAGCGATGGCCGGCGTATCAGTGCCGTTCATGAAGGCTGTATTGGACGGCTACGATGGCTTCGGAGGCGTGGAGAAATTGGTCGACGTCGGCGGCAGCGCCGGTGATTGCTTGAGGATGATCCTACAGAAACATCCTCACATTAAGGAAGGAATCAACTTCGATCTGCCGGAAGTGGTGGCTAGGGCTCCCAGCATCCCCGGGGTGACTCACGTTGGAGGCGACATGTTCAAGTCCGTCCCCACCGGTGACGCTATTTTCATGAAGTGGGTTCTGTCAACATGGACGGATGATGAATGCAAGATGATATTGGAGAACTGCTGCAAGTCACTCCCGGCGGGAGGGAAACTGATCGCCTGCGAGCCGACGGTGCCTGAGAAGACGGACGACAGTCACCGGACACGTGCCCTTTTAGCCAACGACATATTCATCATGACCATTTACAGTGCGAAGAGTAAGCAGAGGACTGAGGATGAGTTCCGGCAGCTGGGCCTCTCCGTCGGCTTCTCCGGCATGCGCTCATTCCACGTGGATTACTTCTATTGTGTGTTGGAATTCCAGAAGTGAAGGCTTGGATGTTATTGCAGAGATTTTAGATTCGGCTGTTTACAATTGGCATTGGAATCTGAACAAATATTTTGATTAGTATTCAAACAATGGTGTAGTGGAAGATTGGTTGGGTTGGGTGGAGCAACCTAACACCCCTCACACACTCTGTGATTTTCTGTCTTCTTCCTCATTGTGGGTTCCAGCCATGGCCCTCCCCTTCCCGCCAGTATTCACCCATCCCCATTCTAATCATATCTTACCCACATTTCCATTTTTATTTCTGATTTTTTGTCATATTAATATCCAAATGAGTTTTTTTTTTTTTTTTTACTTGTTATTTTTTACCGTCTTATTTATATCGAGGTGAGCTGTTTTAACCGTG

Coding sequence (CDS)

ATGGTCGGAAAGGAAGATCCGCCGATGGGGAAAGAATCCGCCAACACCGTTGCCGGAAGCGAGAATCATAAAACAGCGAGGCTAGCCATACTAGAGCTCGCAAACATGATCAGCGTTCCGATGTCCCTCAATGCCGTCGTCCGTCTCAAAATCGCCGACGCTATCTGGCAAGGAGGTTCCAATTTTCCGCTCTCTGCTTCTGAGATCCTCGCACGCTTGGTTCCATCCGGTGGCGACGCCGATAATCTCGAGCGGATCCTCCGTATGCTCACTAGCTATGGCGTTTTCGAAGAGCATCTCAACCCTAACTCGTCGGACCGCCGGTATTCCCTCACCGACGTAGGAAAAACTCTGGTCACCGATTCCGATGGTCTCTCTTACGCGTCGTACGTGCTTCAGCATCATCAGGACGCGCTTATGAGAGCGTGGCCAAGAGTGCACGAGGCTGCGATTGACGCTACTACGGAGCCGTTTGTCAGAGCTAATGGCGAAGCAGCGTACAGTTACTACGGGAAGAATGAGGAGATGAACTTGTTAATGCAGAGAGCGATGGCCGGCGTATCAGTGCCGTTCATGAAGGCTGTATTGGACGGCTACGATGGCTTCGGAGGCGTGGAGAAATTGGTCGACGTCGGCGGCAGCGCCGGTGATTGCTTGAGGATGATCCTACAGAAACATCCTCACATTAAGGAAGGAATCAACTTCGATCTGCCGGAAGTGGTGGCTAGGGCTCCCAGCATCCCCGGGGTGACTCACGTTGGAGGCGACATGTTCAAGTCCGTCCCCACCGGTGACGCTATTTTCATGAAGTGGGTTCTGTCAACATGGACGGATGATGAATGCAAGATGATATTGGAGAACTGCTGCAAGTCACTCCCGGCGGGAGGGAAACTGATCGCCTGCGAGCCGACGGTGCCTGAGAAGACGGACGACAGTCACCGGACACGTGCCCTTTTAGCCAACGACATATTCATCATGACCATTTACAGTGCGAAGAGTAAGCAGAGGACTGAGGATGAGTTCCGGCAGCTGGGCCTCTCCGTCGGCTTCTCCGGCATGCGCTCATTCCACGTGGATTACTTCTATTGTGTGTTGGAATTCCAGAAGTGA

Protein sequence

MVGKEDPPMGKESANTVAGSENHKTARLAILELANMISVPMSLNAVVRLKIADAIWQGGSNFPLSASEILARLVPSGGDADNLERILRMLTSYGVFEEHLNPNSSDRRYSLTDVGKTLVTDSDGLSYASYVLQHHQDALMRAWPRVHEAAIDATTEPFVRANGEAAYSYYGKNEEMNLLMQRAMAGVSVPFMKAVLDGYDGFGGVEKLVDVGGSAGDCLRMILQKHPHIKEGINFDLPEVVARAPSIPGVTHVGGDMFKSVPTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLIACEPTVPEKTDDSHRTRALLANDIFIMTIYSAKSKQRTEDEFRQLGLSVGFSGMRSFHVDYFYCVLEFQK

Homology

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BLAST of CmaCh06G004150.1 vs. ExPASy Swiss-Prot
Match: I1M2U5 (Nicotinate N-methyltransferase 1 OS=Glycine max OX=3847 GN=NANMT1 PE=1 SV=1)

HSP 1 Score: 542.0 bits (1395), Expect = 5.4e-153
Identity = 262/351 (74.64%), Postives = 301/351 (85.75%), Query Frame = 0


Query: 20  SENHKTARLAILELANMISVPMSLNAVVRLKIADAIWQGGSNFPLSASEILARLVPS-GG 79
           +E  K ARLAI+ELANMISVPM+LNAVVRL +ADAIWQGG+N PLSA+EIL RL+P+ GG
Sbjct: 7   TEQRKQARLAIMELANMISVPMALNAVVRLNVADAIWQGGANNPLSAAEILPRLLPAGGG 66

Query: 80  DADNLERILRMLTSYGVFEEHLNPNSSDRRYSLTDVGKTLVTDSDGLSYASYVLQHHQDA 139
           DA+NL+R+LRML SYGVF EHL  ++ +R+YSLTDVGKTLVTD  GLSYA YVLQHHQDA
Sbjct: 67  DAENLQRLLRMLASYGVFYEHL--SAGERKYSLTDVGKTLVTDEQGLSYAHYVLQHHQDA 126

Query: 140 LMRAWPRVHEAAIDATTEPFVRANGEAAYSYYGKNEEMNLLMQRAMAGVSVPFMKAVLDG 199
           LMRAWP VHEA +D T EPF RANGE AY YY K+ EMN LM RAM+GVSVPF++A+L+G
Sbjct: 127 LMRAWPMVHEAVVDPTKEPFERANGEPAYGYYLKHPEMNDLMVRAMSGVSVPFIRAMLEG 186

Query: 200 YDGFGGVEKLVDVGGSAGDCLRMILQKHPHIKEGINFDLPEVVARAPSIPGVTHVGGDMF 259
           YDGF GVEKLVDVGGS GDCLRMIL+KHP IKEGINFDLPEVVA+AP IP VTHVGGDMF
Sbjct: 187 YDGFQGVEKLVDVGGSGGDCLRMILEKHPTIKEGINFDLPEVVAKAPQIPFVTHVGGDMF 246

Query: 260 KSVPTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLIACEPTVPEKTDDSHRTRAL 319
           K +P GDAIFMKWVL+TWTD+ECK I++NC K+LP GGKLIACEP +PE +D+SHRTRAL
Sbjct: 247 KFIPQGDAIFMKWVLTTWTDEECKHIMQNCHKALPEGGKLIACEPVLPEDSDESHRTRAL 306

Query: 320 LANDIFIMTIYSAKSKQRTEDEFRQLGLSVGFSGMRSFHVDYFYCVLEFQK 370
           L  DIF+MTIY AK K RTE++FRQL +  GF   R+FHVD+FY VLEFQK
Sbjct: 307 LEGDIFVMTIYRAKGKHRTEEQFRQLAIDAGFPRFRAFHVDHFYTVLEFQK 355

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BLAST of CmaCh06G004150.1 vs. ExPASy Swiss-Prot
Match: Q9SCP7 (Nicotinate N-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=NANMT1 PE=1 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 2.9e-151
Identity = 261/355 (73.52%), Postives = 305/355 (85.92%), Query Frame = 0


Query: 20  SENHKTARLAILELANMISVPMSLNAVVRLKIADAIWQGGSNFPLSASEILARL-VPS-- 79
           SE+   ARLAI+ELANMISVPMSLNA VRL IADAIW GG+N PLSA+EIL RL +PS  
Sbjct: 6   SESRNRARLAIMELANMISVPMSLNAAVRLGIADAIWNGGANSPLSAAEILPRLHLPSHT 65

Query: 80  --GGDADNLERILRMLTSYGVFEEHLNPNSSDRRYSLTDVGKTLVTDSDGLSYASYVLQH 139
             GGD +NL+RILRMLTSYGVF EHL   S +R+YSLTDVGKTLVTDS GLSYA+YVLQH
Sbjct: 66  TIGGDPENLQRILRMLTSYGVFSEHL-VGSIERKYSLTDVGKTLVTDSGGLSYAAYVLQH 125

Query: 140 HQDALMRAWPRVHEAAIDATTEPFVRANGEAAYSYYGKNEEMNLLMQRAMAGVSVPFMKA 199
           HQ+ALMRAWP VH A ++  TEP+V+ANGEAAY+ YGK+EEMN LMQ+AM+GVSVPFMKA
Sbjct: 126 HQEALMRAWPLVHTAVVEPETEPYVKANGEAAYAQYGKSEEMNGLMQKAMSGVSVPFMKA 185

Query: 200 VLDGYDGFGGVEKLVDVGGSAGDCLRMILQKHPHIKEGINFDLPEVVARAPSIPGVTHVG 259
           +LDGYDGF  V+ LVDVGGSAGDCLRMILQ+ P+++EGINFDLPEVVA+AP+IPGVTHVG
Sbjct: 186 ILDGYDGFKSVDILVDVGGSAGDCLRMILQQFPNVREGINFDLPEVVAKAPNIPGVTHVG 245

Query: 260 GDMFKSVPTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLIACEPTVPEKTDDSHR 319
           GDMF+SVP+ DAIFMKWVL+TWTD+ECK I++NC  +LP GGKLIACEP +P++TD+SHR
Sbjct: 246 GDMFQSVPSADAIFMKWVLTTWTDEECKQIMKNCYNALPVGGKLIACEPVLPKETDESHR 305

Query: 320 TRALLANDIFIMTIYSAKSKQRTEDEFRQLGLSVGFSGMRSFHVDYFYCVLEFQK 370
           TRALL  DIF+MTIY  K K RTE+EF +LGLS GF   R F++DYFY +LEFQK
Sbjct: 306 TRALLEGDIFVMTIYRTKGKHRTEEEFIELGLSAGFPTFRPFYIDYFYTILEFQK 359

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BLAST of CmaCh06G004150.1 vs. ExPASy Swiss-Prot
Match: Q6K9X3 (Nicotinate N-methyltransferase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=NANMT1 PE=1 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 2.6e-115
Identity = 212/356 (59.55%), Postives = 267/356 (75.00%), Query Frame = 0


Query: 26  ARLAILELANMISVPMSLNAVVRLKIADAIWQGGSNFPLSASEILARLVPSG-GDADNLE 85
           ARLA++ELANMISVPM+L AV+RL +   +W GG+N PL+A+++L    P+G  D   LE
Sbjct: 14  ARLAMMELANMISVPMALTAVIRLGVPAKLWAGGANAPLAAADLL----PAGHPDPSVLE 73

Query: 86  RILRMLTSYGVFEEHLNPNS-SDRRYSLTDVGKTLV------TDSDGLSYASYVLQHHQD 145
           R+LR+L S GVF EH   +S S RR+SLT VG+TLV          G SYA YVLQHHQD
Sbjct: 74  RLLRLLASRGVFSEHTGSSSPSPRRFSLTAVGRTLVPGGGGSPSGSGASYADYVLQHHQD 133

Query: 146 ALMRAWPRVHEAAIDAT-TEPFVRAN-GEAAYSYYGKNEEMNLLMQRAMAGVSVPFMKAV 205
           AL+RAWP +HEA +D +  EPF RAN G  AY+YYGK+ E N +M RAM GVS PFM+A+
Sbjct: 134 ALVRAWPLLHEAVLDPSGPEPFARANAGVPAYAYYGKDREANEVMLRAMTGVSEPFMEAL 193

Query: 206 LDGY--DGFGGVEKLVDVGGSAGDCLRMILQKHPHIKEGINFDLPEVVARAPSIPGVTHV 265
           L+GY   GF GV  LVDVGGS+G CL MI+++   I++G+NFDLP+VVA AP IPGV HV
Sbjct: 194 LEGYGDGGFEGVSTLVDVGGSSGACLEMIMRRVRTIRDGVNFDLPDVVAAAPPIPGVRHV 253

Query: 266 GGDMFKSVPTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLIACEPTVPEKTDDSH 325
           GGDMFKS+P+GDAIFMKWVL+TWT++EC  IL NC K+LP GGK+IACEP VP+ TD S 
Sbjct: 254 GGDMFKSIPSGDAIFMKWVLTTWTNEECTAILSNCHKALPGGGKVIACEPVVPDTTDGST 313

Query: 326 RTRALLANDIFIMTIYSAKSKQRTEDEFRQLGLSVGFSGMRSFHVDYFYCVLEFQK 370
           RTRALL NDIF+M  Y  + ++R+E+EFR LGL+ GF+  R+ ++D FY VLE+ K
Sbjct: 314 RTRALLENDIFVMATYRTQGRERSEEEFRHLGLAAGFASFRAIYLDPFYAVLEYTK 365

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BLAST of CmaCh06G004150.1 vs. ExPASy Swiss-Prot
Match: Q6T1F5 (Caffeic acid 3-O-methyltransferase OS=Ammi majus OX=48026 GN=COMT PE=1 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 3.6e-64
Identity = 140/354 (39.55%), Postives = 201/354 (56.78%), Query Frame = 0


Query: 20  SENHKTARLAILELANMISVPMSLNAVVRLKIADAIWQGGSNFPLSASEILARLVPSGGD 79
           +++ + A +  ++LA+   +PM L + + L + ++I + G    +S S++ A L  S  D
Sbjct: 15  NDDEEEACMFAMQLASASVLPMVLKSAIELNLLESIAKAGPGAYVSPSQLAAALPSSQPD 74

Query: 80  AD-NLERILRMLTSYGVFEEHLN--PNSS-DRRYSLTDVGKTLVTDSDGLSYASYVLQHH 139
               L+RILR+L SY V    L   P++  +R Y L  V K L  +SDG+S A  +L + 
Sbjct: 75  TPVMLDRILRLLASYSVLNCKLRDLPDARVERLYGLAPVCKFLTKNSDGVSMAPLLLMNQ 134

Query: 140 QDALMRAWPRVHEAAIDATTEPFVRANGEAAYSYYGKNEEMNLLMQRAMAGVSVPFMKAV 199
              LM +W  + +A +D    PF +A G  A+ Y+GK+   N +  + M+  S   MK +
Sbjct: 135 DKILMESWYHLKDAVLDGGI-PFNKAYGMTAFEYHGKDPRFNKVFNQGMSNHSTITMKKI 194

Query: 200 LDGYDGFGGVEKLVDVGGSAGDCLRMILQKHPHIKEGINFDLPEVVARAPSIPGVTHVGG 259
           L  YDGFGG++ +VDVGG  G  L MI+ K+P++K GINFDLP VV  APS  GV HVGG
Sbjct: 195 LQTYDGFGGLKTVVDVGGGTGATLNMIISKYPNLK-GINFDLPHVVEDAPSYAGVEHVGG 254

Query: 260 DMFKSVPTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLIACEPTVPEKTDDSHRT 319
           DMF SVP GDAIFMKW+   W+D  C   L+NC K+LP  GK+I  E  +PE  D    T
Sbjct: 255 DMFVSVPKGDAIFMKWICHDWSDAHCLAFLKNCYKALPKDGKVILAECILPEAPDSKLTT 314

Query: 320 RALLANDIFIMTIYSAKSKQRTEDEFRQLGLSVGFSGMRSFHVDYFYCVLEFQK 370
           + ++  D+ IM  ++   K+RTE EF   G   GF         Y   V+E+ K
Sbjct: 315 KNVILIDV-IMLAHNPGGKERTEKEFEAFGKQAGFKSFNKACCAYNTWVIEYYK 365

[Close]

BLAST of CmaCh06G004150.1 vs. ExPASy Swiss-Prot
Match: Q8W013 (Caffeic acid 3-O-methyltransferase OS=Catharanthus roseus OX=4058 GN=COMT1 PE=2 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 6.7e-63
Identity = 138/354 (38.98%), Postives = 198/354 (55.93%), Query Frame = 0


Query: 20  SENHKTARLAILELANMISVPMSLNAVVRLKIADAIWQGGSNFPLSASEILARLVPSGGD 79
           ++  + A L+ + LA+   +PM L + + L + + I + G    +S SE+ A+L     D
Sbjct: 13  TKEEEEACLSAMRLASASVLPMVLKSAIELDLLELIKKSGPGAYVSPSELAAQLPTQNPD 72

Query: 80  AD-NLERILRMLTSYGVFE---EHLNPNSSDRRYSLTDVGKTLVTDSDGLSYASYVLQHH 139
           A   L+RILR+L SY V     + L     +R YSL  V K L  + DG+S A+ +L + 
Sbjct: 73  APVMLDRILRLLASYSVLNCTLKDLPDGGIERLYSLAPVCKFLTKNEDGVSMAALLLMNQ 132

Query: 140 QDALMRAWPRVHEAAIDATTEPFVRANGEAAYSYYGKNEEMNLLMQRAMAGVSVPFMKAV 199
              LM +W  + +A ++    PF +A G  A+ Y+GK+   N +  + M+  S   MK +
Sbjct: 133 DKVLMESWYHLKDAVLEGGI-PFNKAYGMTAFEYHGKDPRFNKVFNQGMSNHSTIIMKKI 192

Query: 200 LDGYDGFGGVEKLVDVGGSAGDCLRMILQKHPHIKEGINFDLPEVVARAPSIPGVTHVGG 259
           L+ Y GF G++ +VDVGG  G  L MI+ K+P IK GINFDLP V+  APS PGV HVGG
Sbjct: 193 LEIYQGFQGLKTVVDVGGGTGATLNMIVSKYPSIK-GINFDLPHVIEDAPSYPGVDHVGG 252

Query: 260 DMFKSVPTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLIACEPTVPEKTDDSHRT 319
           DMF SVP GDAIFMKW+   W+D  C   L+NC ++LP  GK+I  E  +PE  D +  T
Sbjct: 253 DMFVSVPKGDAIFMKWICHDWSDAHCLKFLKNCHEALPENGKVILAECLLPEAPDSTLST 312

Query: 320 RALLANDIFIMTIYSAKSKQRTEDEFRQLGLSVGFSGMRSFHVDYFYCVLEFQK 370
           +  +  D+ IM  ++   K+RTE EF  L    GF G       Y   ++E  K
Sbjct: 313 QNTVHVDV-IMLAHNPGGKERTEKEFEALAKGAGFRGFIKVCCAYNSWIMELLK 363

[Close]

BLAST of CmaCh06G004150.1 vs. ExPASy TrEMBL
Match: A0A6J1KU70 (caffeic acid 3-O-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111498706 PE=3 SV=1)

HSP 1 Score: 750.0 bits (1935), Expect = 4.8e-213
Identity = 369/369 (100.00%), Postives = 369/369 (100.00%), Query Frame = 0


Query: 1   MVGKEDPPMGKESANTVAGSENHKTARLAILELANMISVPMSLNAVVRLKIADAIWQGGS 60
           MVGKEDPPMGKESANTVAGSENHKTARLAILELANMISVPMSLNAVVRLKIADAIWQGGS
Sbjct: 1   MVGKEDPPMGKESANTVAGSENHKTARLAILELANMISVPMSLNAVVRLKIADAIWQGGS 60

Query: 61  NFPLSASEILARLVPSGGDADNLERILRMLTSYGVFEEHLNPNSSDRRYSLTDVGKTLVT 120
           NFPLSASEILARLVPSGGDADNLERILRMLTSYGVFEEHLNPNSSDRRYSLTDVGKTLVT
Sbjct: 61  NFPLSASEILARLVPSGGDADNLERILRMLTSYGVFEEHLNPNSSDRRYSLTDVGKTLVT 120

Query: 121 DSDGLSYASYVLQHHQDALMRAWPRVHEAAIDATTEPFVRANGEAAYSYYGKNEEMNLLM 180
           DSDGLSYASYVLQHHQDALMRAWPRVHEAAIDATTEPFVRANGEAAYSYYGKNEEMNLLM
Sbjct: 121 DSDGLSYASYVLQHHQDALMRAWPRVHEAAIDATTEPFVRANGEAAYSYYGKNEEMNLLM 180

Query: 181 QRAMAGVSVPFMKAVLDGYDGFGGVEKLVDVGGSAGDCLRMILQKHPHIKEGINFDLPEV 240
           QRAMAGVSVPFMKAVLDGYDGFGGVEKLVDVGGSAGDCLRMILQKHPHIKEGINFDLPEV
Sbjct: 181 QRAMAGVSVPFMKAVLDGYDGFGGVEKLVDVGGSAGDCLRMILQKHPHIKEGINFDLPEV 240

Query: 241 VARAPSIPGVTHVGGDMFKSVPTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLIA 300
           VARAPSIPGVTHVGGDMFKSVPTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLIA
Sbjct: 241 VARAPSIPGVTHVGGDMFKSVPTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLIA 300

Query: 301 CEPTVPEKTDDSHRTRALLANDIFIMTIYSAKSKQRTEDEFRQLGLSVGFSGMRSFHVDY 360
           CEPTVPEKTDDSHRTRALLANDIFIMTIYSAKSKQRTEDEFRQLGLSVGFSGMRSFHVDY
Sbjct: 301 CEPTVPEKTDDSHRTRALLANDIFIMTIYSAKSKQRTEDEFRQLGLSVGFSGMRSFHVDY 360

Query: 361 FYCVLEFQK 370
           FYCVLEFQK
Sbjct: 361 FYCVLEFQK 369

[Close]

BLAST of CmaCh06G004150.1 vs. ExPASy TrEMBL
Match: A0A6J1G5E3 (caffeic acid 3-O-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111450928 PE=3 SV=1)

HSP 1 Score: 726.1 bits (1873), Expect = 7.4e-206
Identity = 355/369 (96.21%), Postives = 365/369 (98.92%), Query Frame = 0


Query: 1   MVGKEDPPMGKESANTVAGSENHKTARLAILELANMISVPMSLNAVVRLKIADAIWQGGS 60
           +VGKE+PPM KESANTVAGSE+HK ARLAILELANMISVPMSLNAVVRL IADAIWQGGS
Sbjct: 2   VVGKENPPMEKESANTVAGSESHKRARLAILELANMISVPMSLNAVVRLNIADAIWQGGS 61

Query: 61  NFPLSASEILARLVPSGGDADNLERILRMLTSYGVFEEHLNPNSSDRRYSLTDVGKTLVT 120
           NFPLSASEILAR+VPSGGDADNLERILRMLTSYGVFEEHLNPNSSDRRYSLTDVGKTLVT
Sbjct: 62  NFPLSASEILARVVPSGGDADNLERILRMLTSYGVFEEHLNPNSSDRRYSLTDVGKTLVT 121

Query: 121 DSDGLSYASYVLQHHQDALMRAWPRVHEAAIDATTEPFVRANGEAAYSYYGKNEEMNLLM 180
           DSDGLSYASYVLQHHQDALMRAWPRVHEAAIDA+TEPFVRANGEAAYSYYGKNEEMNLLM
Sbjct: 122 DSDGLSYASYVLQHHQDALMRAWPRVHEAAIDASTEPFVRANGEAAYSYYGKNEEMNLLM 181

Query: 181 QRAMAGVSVPFMKAVLDGYDGFGGVEKLVDVGGSAGDCLRMILQKHPHIKEGINFDLPEV 240
           QRAMAGVSVPFMKAVLDGYDGFGGVEKLVDVGGSAGDCLRMILQK+PHIKEGINFDLPEV
Sbjct: 182 QRAMAGVSVPFMKAVLDGYDGFGGVEKLVDVGGSAGDCLRMILQKYPHIKEGINFDLPEV 241

Query: 241 VARAPSIPGVTHVGGDMFKSVPTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLIA 300
           VARAPSIPGVTHVGGDMFKSVPTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLIA
Sbjct: 242 VARAPSIPGVTHVGGDMFKSVPTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLIA 301

Query: 301 CEPTVPEKTDDSHRTRALLANDIFIMTIYSAKSKQRTEDEFRQLGLSVGFSGMRSFHVDY 360
           CEPTVPEKTDDSHRTRALLAND+FIMTIYSAKSKQRTED+FRQLGLSVGFS +RSFHVDY
Sbjct: 302 CEPTVPEKTDDSHRTRALLANDVFIMTIYSAKSKQRTEDQFRQLGLSVGFSALRSFHVDY 361

Query: 361 FYCVLEFQK 370
           FYCVLEF+K
Sbjct: 362 FYCVLEFEK 370

[Close]

BLAST of CmaCh06G004150.1 vs. ExPASy TrEMBL
Match: A0A6J1CSN1 (caffeic acid 3-O-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111014390 PE=3 SV=1)

HSP 1 Score: 661.4 bits (1705), Expect = 2.2e-186
Identity = 322/369 (87.26%), Postives = 344/369 (93.22%), Query Frame = 0


Query: 1   MVGKEDPPMGKESANTVAGSENHKTARLAILELANMISVPMSLNAVVRLKIADAIWQGGS 60
           +VGKE  PMGKE+A     SE+   ARLAILELANMISVPMSLNA+VRL IADAIWQ GS
Sbjct: 2   VVGKEKSPMGKETAE----SESQNRARLAILELANMISVPMSLNAIVRLNIADAIWQEGS 61

Query: 61  NFPLSASEILARLVPSGGDADNLERILRMLTSYGVFEEHLNPNSSDRRYSLTDVGKTLVT 120
           N PLSASEIL+R++PSGGDADNL RILRMLTSYGVFEEHL+ NSS+RRYSLTDVGKTLVT
Sbjct: 62  NDPLSASEILSRVLPSGGDADNLNRILRMLTSYGVFEEHLSHNSSERRYSLTDVGKTLVT 121

Query: 121 DSDGLSYASYVLQHHQDALMRAWPRVHEAAIDATTEPFVRANGEAAYSYYGKNEEMNLLM 180
           DSDGLSYA YVLQHHQDALMRAWPRVHEAA DATTEPFVRANGE AYSYYGK  EMN LM
Sbjct: 122 DSDGLSYAPYVLQHHQDALMRAWPRVHEAASDATTEPFVRANGEPAYSYYGKRPEMNELM 181

Query: 181 QRAMAGVSVPFMKAVLDGYDGFGGVEKLVDVGGSAGDCLRMILQKHPHIKEGINFDLPEV 240
           QRAM+GVSVPFMKA+LDGYDGFGGVEKLVDVGGSAGDCLRMILQKHPHIKEGINFDLPEV
Sbjct: 182 QRAMSGVSVPFMKAILDGYDGFGGVEKLVDVGGSAGDCLRMILQKHPHIKEGINFDLPEV 241

Query: 241 VARAPSIPGVTHVGGDMFKSVPTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLIA 300
           VARAPSIPGVTHVGGDMFKS+PTGDAIFMKWVLSTWTD+ECK+ILENCC+SLPAGGKLIA
Sbjct: 242 VARAPSIPGVTHVGGDMFKSIPTGDAIFMKWVLSTWTDEECKIILENCCRSLPAGGKLIA 301

Query: 301 CEPTVPEKTDDSHRTRALLANDIFIMTIYSAKSKQRTEDEFRQLGLSVGFSGMRSFHVDY 360
           CEPT+PEKTDDSHRTRALLAND+FIMTIY AKSKQRTE++FRQLGLS GFS +R FH+DY
Sbjct: 302 CEPTLPEKTDDSHRTRALLANDVFIMTIYRAKSKQRTEEQFRQLGLSAGFSALRPFHIDY 361

Query: 361 FYCVLEFQK 370
           FYC+LEFQK
Sbjct: 362 FYCLLEFQK 366

[Close]

BLAST of CmaCh06G004150.1 vs. ExPASy TrEMBL
Match: A0A0A0LED0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G747630 PE=3 SV=1)

HSP 1 Score: 659.4 bits (1700), Expect = 8.5e-186
Identity = 318/369 (86.18%), Postives = 343/369 (92.95%), Query Frame = 0


Query: 1   MVGKEDPPMGKESANTVAGSENHKTARLAILELANMISVPMSLNAVVRLKIADAIWQGGS 60
           +VG E PPM + + +T A SE    ARLAI+ELANMISVPMSLNA+VRL +ADAIWQ GS
Sbjct: 2   VVGNETPPMEQHNTSTTADSEIQNKARLAIIELANMISVPMSLNAIVRLNVADAIWQNGS 61

Query: 61  NFPLSASEILARLVPSGGDADNLERILRMLTSYGVFEEHLNPNSSDRRYSLTDVGKTLVT 120
           N PLSASEILAR+VPSGGDA NLERILRMLTSYGVFEEHL+PNSS+ RYSLTDVGKTLVT
Sbjct: 62  NSPLSASEILARVVPSGGDAHNLERILRMLTSYGVFEEHLSPNSSNHRYSLTDVGKTLVT 121

Query: 121 DSDGLSYASYVLQHHQDALMRAWPRVHEAAIDATTEPFVRANGEAAYSYYGKNEEMNLLM 180
           DSDGLSYA YVLQHHQDALMRAWPRVHEAAID+TTEPFVRANGEAAYSYYGK  EMN LM
Sbjct: 122 DSDGLSYAPYVLQHHQDALMRAWPRVHEAAIDSTTEPFVRANGEAAYSYYGKKTEMNELM 181

Query: 181 QRAMAGVSVPFMKAVLDGYDGFGGVEKLVDVGGSAGDCLRMILQKHPHIKEGINFDLPEV 240
           QRAMAGVSVPFMKAVLDGYDGF GVEKLVDVGGSAGDCLRMILQK+PHIKEGINFDLPEV
Sbjct: 182 QRAMAGVSVPFMKAVLDGYDGFKGVEKLVDVGGSAGDCLRMILQKYPHIKEGINFDLPEV 241

Query: 241 VARAPSIPGVTHVGGDMFKSVPTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLIA 300
           VARAP+IPGV+HVGGDMFKS+PTGDAIFMKWVLSTWTDDECK+ILENCCKSLP GGKLIA
Sbjct: 242 VARAPTIPGVSHVGGDMFKSIPTGDAIFMKWVLSTWTDDECKIILENCCKSLPVGGKLIA 301

Query: 301 CEPTVPEKTDDSHRTRALLANDIFIMTIYSAKSKQRTEDEFRQLGLSVGFSGMRSFHVDY 360
           CEPT+PEKTD+SHRTRALLA+D+FIMTIY AKSKQRTE++FRQLGLS GFS +R FH+DY
Sbjct: 302 CEPTLPEKTDESHRTRALLASDVFIMTIYKAKSKQRTEEQFRQLGLSAGFSALRPFHIDY 361

Query: 361 FYCVLEFQK 370
           FYC+LEFQK
Sbjct: 362 FYCLLEFQK 370

[Close]

BLAST of CmaCh06G004150.1 vs. ExPASy TrEMBL
Match: A0A5A7TKW0 (Caffeic acid 3-O-methyltransferase isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G002960 PE=3 SV=1)

HSP 1 Score: 642.9 bits (1657), Expect = 8.2e-181
Identity = 314/370 (84.86%), Postives = 339/370 (91.62%), Query Frame = 0


Query: 1   MVGKEDPPMGKESANTVAGSENHKTARLAILELANMISVPMSLNAVVRLKIADAIWQGGS 60
           +VGKE  PM + +A+T A SE    ARLAILELANMISVPMSLNA+VRL +ADAIWQ GS
Sbjct: 2   VVGKETAPMEQHNASTTADSEIQNKARLAILELANMISVPMSLNAIVRLNVADAIWQNGS 61

Query: 61  NFPLSASEILARLVPS-GGDADNLERILRMLTSYGVFEEHLNPNSSDRRYSLTDVGKTLV 120
           N PLSASEILAR+VPS GGDA NLERILRMLTSYGVFEEHL+PNSS+ RYSLTDVGKTLV
Sbjct: 62  NSPLSASEILARVVPSGGGDAHNLERILRMLTSYGVFEEHLSPNSSNHRYSLTDVGKTLV 121

Query: 121 TDSDGLSYASYVLQHHQDALMRAWPRVHEAAIDATTEPFVRANGEAAYSYYGKNEEMNLL 180
           TD+DGLSYA YVLQHHQDALMRAWPRVHEAAID+TTEPFVRANGE AYSYYGK  EMN L
Sbjct: 122 TDTDGLSYAPYVLQHHQDALMRAWPRVHEAAIDSTTEPFVRANGEPAYSYYGKKTEMNEL 181

Query: 181 MQRAMAGVSVPFMKAVLDGYDGFGGVEKLVDVGGSAGDCLRMILQKHPHIKEGINFDLPE 240
           MQRAMAGVSVPFMKAVLDGYDGF GVEKLVDVGGSAGDCLRMILQK+P IKEGINFDLPE
Sbjct: 182 MQRAMAGVSVPFMKAVLDGYDGFKGVEKLVDVGGSAGDCLRMILQKYPSIKEGINFDLPE 241

Query: 241 VVARAPSIPGVTHVGGDMFKSVPTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLI 300
           VVARAP IPGV+HVGGDMFKS+PT DA+FMKWVLSTWTDDECK+ILENCCKSLP GGKLI
Sbjct: 242 VVARAPPIPGVSHVGGDMFKSIPTADAMFMKWVLSTWTDDECKIILENCCKSLPVGGKLI 301

Query: 301 ACEPTVPEKTDDSHRTRALLANDIFIMTIYSAKSKQRTEDEFRQLGLSVGFSGMRSFHVD 360
           ACEPT+PEKTD+SHRTRALLA+D+FIMTIY AKSKQRTE++FRQLGLS GFS +R FH+D
Sbjct: 302 ACEPTLPEKTDESHRTRALLASDVFIMTIYKAKSKQRTEEQFRQLGLSAGFSALRPFHID 361

Query: 361 YFYCVLEFQK 370
           YFYC+LEF K
Sbjct: 362 YFYCLLEFTK 371

[Close]

BLAST of CmaCh06G004150.1 vs. NCBI nr
Match: XP_023005812.1 (caffeic acid 3-O-methyltransferase [Cucurbita maxima])

HSP 1 Score: 750.0 bits (1935), Expect = 9.9e-213
Identity = 369/369 (100.00%), Postives = 369/369 (100.00%), Query Frame = 0


Query: 1   MVGKEDPPMGKESANTVAGSENHKTARLAILELANMISVPMSLNAVVRLKIADAIWQGGS 60
           MVGKEDPPMGKESANTVAGSENHKTARLAILELANMISVPMSLNAVVRLKIADAIWQGGS
Sbjct: 1   MVGKEDPPMGKESANTVAGSENHKTARLAILELANMISVPMSLNAVVRLKIADAIWQGGS 60

Query: 61  NFPLSASEILARLVPSGGDADNLERILRMLTSYGVFEEHLNPNSSDRRYSLTDVGKTLVT 120
           NFPLSASEILARLVPSGGDADNLERILRMLTSYGVFEEHLNPNSSDRRYSLTDVGKTLVT
Sbjct: 61  NFPLSASEILARLVPSGGDADNLERILRMLTSYGVFEEHLNPNSSDRRYSLTDVGKTLVT 120

Query: 121 DSDGLSYASYVLQHHQDALMRAWPRVHEAAIDATTEPFVRANGEAAYSYYGKNEEMNLLM 180
           DSDGLSYASYVLQHHQDALMRAWPRVHEAAIDATTEPFVRANGEAAYSYYGKNEEMNLLM
Sbjct: 121 DSDGLSYASYVLQHHQDALMRAWPRVHEAAIDATTEPFVRANGEAAYSYYGKNEEMNLLM 180

Query: 181 QRAMAGVSVPFMKAVLDGYDGFGGVEKLVDVGGSAGDCLRMILQKHPHIKEGINFDLPEV 240
           QRAMAGVSVPFMKAVLDGYDGFGGVEKLVDVGGSAGDCLRMILQKHPHIKEGINFDLPEV
Sbjct: 181 QRAMAGVSVPFMKAVLDGYDGFGGVEKLVDVGGSAGDCLRMILQKHPHIKEGINFDLPEV 240

Query: 241 VARAPSIPGVTHVGGDMFKSVPTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLIA 300
           VARAPSIPGVTHVGGDMFKSVPTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLIA
Sbjct: 241 VARAPSIPGVTHVGGDMFKSVPTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLIA 300

Query: 301 CEPTVPEKTDDSHRTRALLANDIFIMTIYSAKSKQRTEDEFRQLGLSVGFSGMRSFHVDY 360
           CEPTVPEKTDDSHRTRALLANDIFIMTIYSAKSKQRTEDEFRQLGLSVGFSGMRSFHVDY
Sbjct: 301 CEPTVPEKTDDSHRTRALLANDIFIMTIYSAKSKQRTEDEFRQLGLSVGFSGMRSFHVDY 360

Query: 361 FYCVLEFQK 370
           FYCVLEFQK
Sbjct: 361 FYCVLEFQK 369

[Close]

BLAST of CmaCh06G004150.1 vs. NCBI nr
Match: XP_023540354.1 (caffeic acid 3-O-methyltransferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 729.6 bits (1882), Expect = 1.4e-206
Identity = 357/369 (96.75%), Postives = 365/369 (98.92%), Query Frame = 0


Query: 1   MVGKEDPPMGKESANTVAGSENHKTARLAILELANMISVPMSLNAVVRLKIADAIWQGGS 60
           +VGKE+PPM KESANTVAGSE+HK ARLAILELANMISVPMSLNAVVRL IADAIWQGGS
Sbjct: 2   VVGKENPPMEKESANTVAGSESHKRARLAILELANMISVPMSLNAVVRLNIADAIWQGGS 61

Query: 61  NFPLSASEILARLVPSGGDADNLERILRMLTSYGVFEEHLNPNSSDRRYSLTDVGKTLVT 120
           NFPLSASEILAR+VPSGGDADNLERILRMLTSYGVFEEHLNPNSSDRRYSLTDVGKTLVT
Sbjct: 62  NFPLSASEILARVVPSGGDADNLERILRMLTSYGVFEEHLNPNSSDRRYSLTDVGKTLVT 121

Query: 121 DSDGLSYASYVLQHHQDALMRAWPRVHEAAIDATTEPFVRANGEAAYSYYGKNEEMNLLM 180
           DSDGLSYASYVLQHHQDALMRAWPRVHEAAIDA+TEPFVRANGEAAYSYYGKNEEMNLLM
Sbjct: 122 DSDGLSYASYVLQHHQDALMRAWPRVHEAAIDASTEPFVRANGEAAYSYYGKNEEMNLLM 181

Query: 181 QRAMAGVSVPFMKAVLDGYDGFGGVEKLVDVGGSAGDCLRMILQKHPHIKEGINFDLPEV 240
           QRAMAGVSVPFMKAVLDGYDGFGGVEKLVDVGGSAGDCLRMILQKHPHIKEGINFDLPEV
Sbjct: 182 QRAMAGVSVPFMKAVLDGYDGFGGVEKLVDVGGSAGDCLRMILQKHPHIKEGINFDLPEV 241

Query: 241 VARAPSIPGVTHVGGDMFKSVPTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLIA 300
           VARAPSIPGVTHVGGDMFKSVPTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLIA
Sbjct: 242 VARAPSIPGVTHVGGDMFKSVPTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLIA 301

Query: 301 CEPTVPEKTDDSHRTRALLANDIFIMTIYSAKSKQRTEDEFRQLGLSVGFSGMRSFHVDY 360
           CEPTVPEKTDDSHRTRALLAND+FIMTIYSAKSKQRTED+FRQLGLSVGFS MRSFHVDY
Sbjct: 302 CEPTVPEKTDDSHRTRALLANDVFIMTIYSAKSKQRTEDQFRQLGLSVGFSAMRSFHVDY 361

Query: 361 FYCVLEFQK 370
           FYCVLEF+K
Sbjct: 362 FYCVLEFEK 370

[Close]

BLAST of CmaCh06G004150.1 vs. NCBI nr
Match: KAG6596509.1 (Caffeic acid 3-O-methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 726.9 bits (1875), Expect = 9.0e-206
Identity = 355/369 (96.21%), Postives = 365/369 (98.92%), Query Frame = 0


Query: 1   MVGKEDPPMGKESANTVAGSENHKTARLAILELANMISVPMSLNAVVRLKIADAIWQGGS 60
           +VGKE+PPM KESANTVAGSE+HK ARLAILELANMISVPMSLNAVVRL IADAIWQGGS
Sbjct: 2   VVGKENPPMEKESANTVAGSESHKRARLAILELANMISVPMSLNAVVRLNIADAIWQGGS 61

Query: 61  NFPLSASEILARLVPSGGDADNLERILRMLTSYGVFEEHLNPNSSDRRYSLTDVGKTLVT 120
           NFPLSASEILAR+VPSGGDADNLERILRMLTSYGVFEEHLNPNSSDRRYSLTDVGKTLVT
Sbjct: 62  NFPLSASEILARVVPSGGDADNLERILRMLTSYGVFEEHLNPNSSDRRYSLTDVGKTLVT 121

Query: 121 DSDGLSYASYVLQHHQDALMRAWPRVHEAAIDATTEPFVRANGEAAYSYYGKNEEMNLLM 180
           DSDGLSYASYVLQHHQDALMRAWPRVHEAAIDA+TEPFVRANGEAAYSYYGKNEEMNLLM
Sbjct: 122 DSDGLSYASYVLQHHQDALMRAWPRVHEAAIDASTEPFVRANGEAAYSYYGKNEEMNLLM 181

Query: 181 QRAMAGVSVPFMKAVLDGYDGFGGVEKLVDVGGSAGDCLRMILQKHPHIKEGINFDLPEV 240
           QRAMAGVSVPFMKAVLDGYDGFGGVEKLVDVGGSAGDCLRMILQK+PHIKEGINFDLPEV
Sbjct: 182 QRAMAGVSVPFMKAVLDGYDGFGGVEKLVDVGGSAGDCLRMILQKYPHIKEGINFDLPEV 241

Query: 241 VARAPSIPGVTHVGGDMFKSVPTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLIA 300
           VARAPSIPGVTHVGGDMFKS+PTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLIA
Sbjct: 242 VARAPSIPGVTHVGGDMFKSIPTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLIA 301

Query: 301 CEPTVPEKTDDSHRTRALLANDIFIMTIYSAKSKQRTEDEFRQLGLSVGFSGMRSFHVDY 360
           CEPTVPEKTDDSHRTRALLAND+FIMTIYSAKSKQRTEDEFRQLGLSVGFS +RSFHVDY
Sbjct: 302 CEPTVPEKTDDSHRTRALLANDVFIMTIYSAKSKQRTEDEFRQLGLSVGFSALRSFHVDY 361

Query: 361 FYCVLEFQK 370
           FYCVLEF+K
Sbjct: 362 FYCVLEFEK 370

[Close]

BLAST of CmaCh06G004150.1 vs. NCBI nr
Match: XP_022946930.1 (caffeic acid 3-O-methyltransferase [Cucurbita moschata])

HSP 1 Score: 726.1 bits (1873), Expect = 1.5e-205
Identity = 355/369 (96.21%), Postives = 365/369 (98.92%), Query Frame = 0


Query: 1   MVGKEDPPMGKESANTVAGSENHKTARLAILELANMISVPMSLNAVVRLKIADAIWQGGS 60
           +VGKE+PPM KESANTVAGSE+HK ARLAILELANMISVPMSLNAVVRL IADAIWQGGS
Sbjct: 2   VVGKENPPMEKESANTVAGSESHKRARLAILELANMISVPMSLNAVVRLNIADAIWQGGS 61

Query: 61  NFPLSASEILARLVPSGGDADNLERILRMLTSYGVFEEHLNPNSSDRRYSLTDVGKTLVT 120
           NFPLSASEILAR+VPSGGDADNLERILRMLTSYGVFEEHLNPNSSDRRYSLTDVGKTLVT
Sbjct: 62  NFPLSASEILARVVPSGGDADNLERILRMLTSYGVFEEHLNPNSSDRRYSLTDVGKTLVT 121

Query: 121 DSDGLSYASYVLQHHQDALMRAWPRVHEAAIDATTEPFVRANGEAAYSYYGKNEEMNLLM 180
           DSDGLSYASYVLQHHQDALMRAWPRVHEAAIDA+TEPFVRANGEAAYSYYGKNEEMNLLM
Sbjct: 122 DSDGLSYASYVLQHHQDALMRAWPRVHEAAIDASTEPFVRANGEAAYSYYGKNEEMNLLM 181

Query: 181 QRAMAGVSVPFMKAVLDGYDGFGGVEKLVDVGGSAGDCLRMILQKHPHIKEGINFDLPEV 240
           QRAMAGVSVPFMKAVLDGYDGFGGVEKLVDVGGSAGDCLRMILQK+PHIKEGINFDLPEV
Sbjct: 182 QRAMAGVSVPFMKAVLDGYDGFGGVEKLVDVGGSAGDCLRMILQKYPHIKEGINFDLPEV 241

Query: 241 VARAPSIPGVTHVGGDMFKSVPTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLIA 300
           VARAPSIPGVTHVGGDMFKSVPTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLIA
Sbjct: 242 VARAPSIPGVTHVGGDMFKSVPTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLIA 301

Query: 301 CEPTVPEKTDDSHRTRALLANDIFIMTIYSAKSKQRTEDEFRQLGLSVGFSGMRSFHVDY 360
           CEPTVPEKTDDSHRTRALLAND+FIMTIYSAKSKQRTED+FRQLGLSVGFS +RSFHVDY
Sbjct: 302 CEPTVPEKTDDSHRTRALLANDVFIMTIYSAKSKQRTEDQFRQLGLSVGFSALRSFHVDY 361

Query: 361 FYCVLEFQK 370
           FYCVLEF+K
Sbjct: 362 FYCVLEFEK 370

[Close]

BLAST of CmaCh06G004150.1 vs. NCBI nr
Match: KAG7028050.1 (Caffeic acid 3-O-methyltransferase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 713.4 bits (1840), Expect = 1.0e-201
Identity = 349/361 (96.68%), Postives = 357/361 (98.89%), Query Frame = 0


Query: 9   MGKESANTVAGSENHKTARLAILELANMISVPMSLNAVVRLKIADAIWQGGSNFPLSASE 68
           M KESANTVAGSE+HK ARLAILELANMISVPMSLNAVVRL IADAIWQGGSNFPLSASE
Sbjct: 1   MEKESANTVAGSESHKRARLAILELANMISVPMSLNAVVRLNIADAIWQGGSNFPLSASE 60

Query: 69  ILARLVPSGGDADNLERILRMLTSYGVFEEHLNPNSSDRRYSLTDVGKTLVTDSDGLSYA 128
           ILAR+VPSGGDADNLERILRMLTSYGVFEEHLNPNSSDRRYSLTDVGKTLVTDSDGLSYA
Sbjct: 61  ILARVVPSGGDADNLERILRMLTSYGVFEEHLNPNSSDRRYSLTDVGKTLVTDSDGLSYA 120

Query: 129 SYVLQHHQDALMRAWPRVHEAAIDATTEPFVRANGEAAYSYYGKNEEMNLLMQRAMAGVS 188
           SYVLQHHQDALMRAWPRVHEAAIDA+TEPFVRANGEAAYSYYGKNEEMNLLMQRAMAGVS
Sbjct: 121 SYVLQHHQDALMRAWPRVHEAAIDASTEPFVRANGEAAYSYYGKNEEMNLLMQRAMAGVS 180

Query: 189 VPFMKAVLDGYDGFGGVEKLVDVGGSAGDCLRMILQKHPHIKEGINFDLPEVVARAPSIP 248
           VPFMKAVLDGYDGFGGVEKLVDVGGSAGDCLRMILQK+PHIKEGINFDLPEVVARAPSIP
Sbjct: 181 VPFMKAVLDGYDGFGGVEKLVDVGGSAGDCLRMILQKYPHIKEGINFDLPEVVARAPSIP 240

Query: 249 GVTHVGGDMFKSVPTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLIACEPTVPEK 308
           GVTHVGGDMFKS+PTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLIACEPTVPEK
Sbjct: 241 GVTHVGGDMFKSIPTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLIACEPTVPEK 300

Query: 309 TDDSHRTRALLANDIFIMTIYSAKSKQRTEDEFRQLGLSVGFSGMRSFHVDYFYCVLEFQ 368
           TDDSHRTRALLAND+FIMTIYSAKSKQRTEDEFRQLGLSVGFS +RSFHVDYFYCVLEF+
Sbjct: 301 TDDSHRTRALLANDVFIMTIYSAKSKQRTEDEFRQLGLSVGFSALRSFHVDYFYCVLEFE 360

Query: 369 K 370
           K
Sbjct: 361 K 361

[Close]

BLAST of CmaCh06G004150.1 vs. TAIR 10
Match: AT3G53140.1 (O-methyltransferase family protein )

HSP 1 Score: 536.2 bits (1380), Expect = 2.1e-152
Identity = 261/355 (73.52%), Postives = 305/355 (85.92%), Query Frame = 0


Query: 20  SENHKTARLAILELANMISVPMSLNAVVRLKIADAIWQGGSNFPLSASEILARL-VPS-- 79
           SE+   ARLAI+ELANMISVPMSLNA VRL IADAIW GG+N PLSA+EIL RL +PS  
Sbjct: 6   SESRNRARLAIMELANMISVPMSLNAAVRLGIADAIWNGGANSPLSAAEILPRLHLPSHT 65

Query: 80  --GGDADNLERILRMLTSYGVFEEHLNPNSSDRRYSLTDVGKTLVTDSDGLSYASYVLQH 139
             GGD +NL+RILRMLTSYGVF EHL   S +R+YSLTDVGKTLVTDS GLSYA+YVLQH
Sbjct: 66  TIGGDPENLQRILRMLTSYGVFSEHL-VGSIERKYSLTDVGKTLVTDSGGLSYAAYVLQH 125

Query: 140 HQDALMRAWPRVHEAAIDATTEPFVRANGEAAYSYYGKNEEMNLLMQRAMAGVSVPFMKA 199
           HQ+ALMRAWP VH A ++  TEP+V+ANGEAAY+ YGK+EEMN LMQ+AM+GVSVPFMKA
Sbjct: 126 HQEALMRAWPLVHTAVVEPETEPYVKANGEAAYAQYGKSEEMNGLMQKAMSGVSVPFMKA 185

Query: 200 VLDGYDGFGGVEKLVDVGGSAGDCLRMILQKHPHIKEGINFDLPEVVARAPSIPGVTHVG 259
           +LDGYDGF  V+ LVDVGGSAGDCLRMILQ+ P+++EGINFDLPEVVA+AP+IPGVTHVG
Sbjct: 186 ILDGYDGFKSVDILVDVGGSAGDCLRMILQQFPNVREGINFDLPEVVAKAPNIPGVTHVG 245

Query: 260 GDMFKSVPTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLIACEPTVPEKTDDSHR 319
           GDMF+SVP+ DAIFMKWVL+TWTD+ECK I++NC  +LP GGKLIACEP +P++TD+SHR
Sbjct: 246 GDMFQSVPSADAIFMKWVLTTWTDEECKQIMKNCYNALPVGGKLIACEPVLPKETDESHR 305

Query: 320 TRALLANDIFIMTIYSAKSKQRTEDEFRQLGLSVGFSGMRSFHVDYFYCVLEFQK 370
           TRALL  DIF+MTIY  K K RTE+EF +LGLS GF   R F++DYFY +LEFQK
Sbjct: 306 TRALLEGDIFVMTIYRTKGKHRTEEEFIELGLSAGFPTFRPFYIDYFYTILEFQK 359

[Close]

BLAST of CmaCh06G004150.1 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 242.3 bits (617), Expect = 6.2e-64
Identity = 135/333 (40.54%), Postives = 197/333 (59.16%), Query Frame = 0


Query: 26  ARLAILELANMISVPMSLNAVVRLKIADAIWQGGSNFPLSASEILARLVPSGGDAD-NLE 85
           A L  ++LA+   +PM+L + + L + + + + GS  P+S +EI ++L     +A   L+
Sbjct: 19  AALFAMQLASASVLPMALKSALELDLLEIMAKNGS--PMSPTEIASKLPTKNPEAPVMLD 78

Query: 86  RILRMLTSYGVF---EEHLNPNSSDRRYSLTDVGKTLVTDSDGLSYASYVLQHHQDALMR 145
           RILR+LTSY V       L+ +  +R Y L  V K L  + DG+S A+  L +    LM 
Sbjct: 79  RILRLLTSYSVLTCSNRKLSGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLME 138

Query: 146 AWPRVHEAAIDATTEPFVRANGEAAYSYYGKNEEMNLLMQRAMAGVSVPFMKAVLDGYDG 205
           +W  + +A +D    PF +A G +A+ Y+G +   N +    M+  S   MK +L+ Y G
Sbjct: 139 SWYHLKDAILDGGI-PFNKAYGMSAFEYHGTDPRFNKVFNNGMSNHSTITMKKILETYKG 198

Query: 206 FGGVEKLVDVGGSAGDCLRMILQKHPHIKEGINFDLPEVVARAPSIPGVTHVGGDMFKSV 265
           F G+  LVDVGG  G  L+MI+ K+P++K GINFDLP V+  APS PG+ HVGGDMF SV
Sbjct: 199 FEGLTSLVDVGGGIGATLKMIVSKYPNLK-GINFDLPHVIEDAPSHPGIEHVGGDMFVSV 258

Query: 266 PTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLIACEPTVPEKTDDSHRTRALLAN 325
           P GDAIFMKW+   W+D+ C   L+NC +SLP  GK+I  E  +PE  D S  T+ ++  
Sbjct: 259 PKGDAIFMKWICHDWSDEHCVKFLKNCYESLPEDGKVILAECILPETPDSSLSTKQVVHV 318

Query: 326 DIFIMTIYSAKSKQRTEDEFRQLGLSVGFSGMR 355
           D  IM  ++   K+RTE EF  L  + GF G++
Sbjct: 319 DC-IMLAHNPGGKERTEKEFEALAKASGFKGIK 346

[Close]

BLAST of CmaCh06G004150.1 vs. TAIR 10
Match: AT1G77530.1 (O-methyltransferase family protein )

HSP 1 Score: 209.9 bits (533), Expect = 3.4e-54
Identity = 131/362 (36.19%), Postives = 191/362 (52.76%), Query Frame = 0


Query: 22  NHKTARLAILELANMISVPMSLNAVVRLKIADAIWQGGSNFPLSASEILARL--VPSGGD 81
           + K   L    + N ++ PM L A + L + D I    +   LS SEI   L   P+  +
Sbjct: 25  DEKMVSLQAESIVNAVAFPMVLKAALELGVIDTIAAASNGTWLSPSEIAVSLPNKPTNPE 84

Query: 82  AD-NLERILRMLTSYGVFE-------EHLNPNSSDRRYSLTDVGKTLVTDSDGL-SYASY 141
           A   L+R+LR+L S+ + +       E+      +R Y+   + K  + DSDG  S +S 
Sbjct: 85  APVLLDRMLRLLVSHSILKCCMVESRENGQTGKIERVYAAEPICKYFLKDSDGSGSLSSL 144

Query: 142 VLQHHQDALMRAWPRVHEAAIDATTEPFVRANGEAAYSYYGKNEEMNLLMQRAMAGVSVP 201
           +L  H   +++ W  + +  ++   + F  A+    + Y   +++ + L  RAM+  S  
Sbjct: 145 LLLLHSQVILKTWTNLKDVILEG-KDAFSSAHDMRLFEYISSDDQFSKLFHRAMSESSTM 204

Query: 202 FMKAVLDGYDGFGGVEKLVDVGGSAGDCLRMILQKHPHIKEGINFDLPEVVARAPSIPGV 261
            MK VL+ Y GF  V  LVDVGG  G  L +I  K+PHIK G+NFDL +V+ +AP  PGV
Sbjct: 205 VMKKVLEEYRGFEDVNTLVDVGGGIGTILGLITSKYPHIK-GVNFDLAQVLTQAPFYPGV 264

Query: 262 THVGGDMFKSVPTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLIACE---PTVPE 321
            HV GDMF  VP GDAIFMKW+L  W D++C  IL+NC KSLP  GK+I  E   P  P+
Sbjct: 265 KHVSGDMFIEVPKGDAIFMKWILHDWGDEDCIKILKNCWKSLPEKGKVIIVEMITPMEPK 324

Query: 322 KTDDSHRTRALLANDIFIMTIYSAKSKQRTEDEFRQLGLSVGFSGMRSFHVDYFYCVLEF 370
             D S  T  +L  D+ ++T  S   K+R+  +F  L  + GF       + Y Y V+EF
Sbjct: 325 PNDFSCNT--VLGMDLLMLTQCSG-GKERSLSQFENLAFASGFLLCEIICLSYSYSVIEF 381

[Close]

BLAST of CmaCh06G004150.1 vs. TAIR 10
Match: AT1G51990.1 (O-methyltransferase family protein )

HSP 1 Score: 209.9 bits (533), Expect = 3.4e-54
Identity = 131/362 (36.19%), Postives = 184/362 (50.83%), Query Frame = 0


Query: 13  SANTVAGSENHKTARLAILELANMISVPMSLNAVVRLKIADAIWQG---GSNFPLSASEI 72
           S  T  GS   +   LAI +L  +  VP  +     L + + + +    GS   LS  ++
Sbjct: 3   SLQTSGGSSEEEDMLLAI-QLGGLNFVPYIVKTARELDLFEIMAKARPLGSY--LSPVDL 62

Query: 73  LARLVPSGGDAD-NLERILRMLTSYGVFEEHL---NPNSSDRRYSLTDVGKTLVTDSDGL 132
            +   P    A   ++R+LR L +Y V    L         R Y L  VGK L+ D DG 
Sbjct: 63  ASMAAPKNPHAPMMIDRLLRFLVAYSVCTCKLVKDEEGRESRAYGLGKVGKKLIKDEDGF 122

Query: 133 SYASYVLQHHQDALMRAWPRVHEAAIDATTEPFVRANGEAAYSYYGKNEEMNLLMQRAMA 192
           S A YVL     A    W  + EA  +     + RAN    + Y  KNE +  +   +M 
Sbjct: 123 SIAPYVLAGCTKAKGGVWSYLTEAIQEGGASAWERANEALIFEYMKKNENLKKIFNESMT 182

Query: 193 GVSVPFMKAVLDGYDGFGGVEKLVDVGGSAGDCLRMILQKHPHIKEGINFDLPEVVARAP 252
             +   MK +L+ Y GF GV   VDVGGS G  L  IL K+PHIK GINFDLP +V  AP
Sbjct: 183 NHTSIVMKKILENYIGFEGVSDFVDVGGSLGSNLAQILSKYPHIK-GINFDLPHIVKEAP 242

Query: 253 SIPGVTHVGGDMFKSVPTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLIACEPTV 312
            I GV H+GGDMF  +P G+ I MKW+L  W D++C  IL+NC K+LP  G++I  E  V
Sbjct: 243 QIHGVEHIGGDMFDEIPRGEVILMKWILHDWNDEKCVEILKNCKKALPETGRIIVIEMIV 302

Query: 313 PEKTDDSH-RTRALLANDIFIMTIYSAKSKQRTEDEFRQLGLSVGFSGMRSFHVDYFYCV 367
           P +  ++   T+  L+ D+ +M++ S   K+RT+ EF  L    GF   +  +  Y Y +
Sbjct: 303 PREVSETDLATKNSLSADLTMMSLTSG-GKERTKKEFEDLAKEAGFKLPKIIYGAYSYWI 359

[Close]

BLAST of CmaCh06G004150.1 vs. TAIR 10
Match: AT1G51990.2 (O-methyltransferase family protein )

HSP 1 Score: 209.9 bits (533), Expect = 3.4e-54
Identity = 131/362 (36.19%), Postives = 183/362 (50.55%), Query Frame = 0


Query: 13  SANTVAGSENHKTARLAILELANMISVPMSLNAVVRLKIADAIWQG---GSNFPLSASEI 72
           S  T  GS   +   LAI +L  +  VP  +     L + + + +    GS   LS  ++
Sbjct: 3   SLQTSGGSSEEEDMLLAI-QLGGLNFVPYIVKTARELDLFEIMAKARPLGSY--LSPVDL 62

Query: 73  LARLVPSGGDAD-NLERILRMLTSYGVFEEHL---NPNSSDRRYSLTDVGKTLVTDSDGL 132
            +   P    A   ++R+LR L +Y V    L         R Y L  VGK L+ D DG 
Sbjct: 63  ASMAAPKNPHAPMMIDRLLRFLVAYSVCTCKLVKDEEGRESRAYGLGKVGKKLIKDEDGF 122

Query: 133 SYASYVLQHHQDALMRAWPRVHEAAIDATTEPFVRANGEAAYSYYGKNEEMNLLMQRAMA 192
           S A YVL     A    W  V  A  +     + RAN    + Y  KNE +  +   +M 
Sbjct: 123 SIAPYVLAGCTKAKGGVWYNVQHAIQEGGASAWERANEALIFEYMKKNENLKKIFNESMT 182

Query: 193 GVSVPFMKAVLDGYDGFGGVEKLVDVGGSAGDCLRMILQKHPHIKEGINFDLPEVVARAP 252
             +   MK +L+ Y GF GV   VDVGGS G  L  IL K+PHIK GINFDLP +V  AP
Sbjct: 183 NHTSIVMKKILENYIGFEGVSDFVDVGGSLGSNLAQILSKYPHIK-GINFDLPHIVKEAP 242

Query: 253 SIPGVTHVGGDMFKSVPTGDAIFMKWVLSTWTDDECKMILENCCKSLPAGGKLIACEPTV 312
            I GV H+GGDMF  +P G+ I MKW+L  W D++C  IL+NC K+LP  G++I  E  V
Sbjct: 243 QIHGVEHIGGDMFDEIPRGEVILMKWILHDWNDEKCVEILKNCKKALPETGRIIVIEMIV 302

Query: 313 PEKTDDSH-RTRALLANDIFIMTIYSAKSKQRTEDEFRQLGLSVGFSGMRSFHVDYFYCV 367
           P +  ++   T+  L+ D+ +M++ S   K+RT+ EF  L    GF   +  +  Y Y +
Sbjct: 303 PREVSETDLATKNSLSADLTMMSLTSG-GKERTKKEFEDLAKEAGFKLPKIIYGAYSYWI 359

The following BLAST results are available for this feature:

BLAST of CmaCh06G004150.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
I1M2U5	5.4e-153	74.64	Nicotinate N-methyltransferase 1 OS=Glycine max OX=3847 GN=NANMT1 PE=1 SV=1	[more]
Q9SCP7	2.9e-151	73.52	Nicotinate N-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=NANMT1 PE=1 ...	[more]
Q6K9X3	2.6e-115	59.55	Nicotinate N-methyltransferase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=NAN...	[more]
Q6T1F5	3.6e-64	39.55	Caffeic acid 3-O-methyltransferase OS=Ammi majus OX=48026 GN=COMT PE=1 SV=1	[more]
Q8W013	6.7e-63	38.98	Caffeic acid 3-O-methyltransferase OS=Catharanthus roseus OX=4058 GN=COMT1 PE=2 ...	[more]

BLAST of CmaCh06G004150.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KU70	4.8e-213	100.00	caffeic acid 3-O-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111498706 P...	[more]
A0A6J1G5E3	7.4e-206	96.21	caffeic acid 3-O-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111450928...	[more]
A0A6J1CSN1	2.2e-186	87.26	caffeic acid 3-O-methyltransferase OS=Momordica charantia OX=3673 GN=LOC11101439...	[more]
A0A0A0LED0	8.5e-186	86.18	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G747630 PE=3 SV=1	[more]
A0A5A7TKW0	8.2e-181	84.86	Caffeic acid 3-O-methyltransferase isoform X2 OS=Cucumis melo var. makuwa OX=119...	[more]

BLAST of CmaCh06G004150.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023005812.1	9.9e-213	100.00	caffeic acid 3-O-methyltransferase [Cucurbita maxima]	[more]
XP_023540354.1	1.4e-206	96.75	caffeic acid 3-O-methyltransferase [Cucurbita pepo subsp. pepo]	[more]
KAG6596509.1	9.0e-206	96.21	Caffeic acid 3-O-methyltransferase, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_022946930.1	1.5e-205	96.21	caffeic acid 3-O-methyltransferase [Cucurbita moschata]	[more]
KAG7028050.1	1.0e-201	96.68	Caffeic acid 3-O-methyltransferase, partial [Cucurbita argyrosperma subsp. argyr...	[more]

BLAST of CmaCh06G004150.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G53140.1	2.1e-152	73.52	O-methyltransferase family protein	[more]
AT5G54160.1	6.2e-64	40.54	O-methyltransferase 1	[more]
AT1G77530.1	3.4e-54	36.19	O-methyltransferase family protein	[more]
AT1G51990.1	3.4e-54	36.19	O-methyltransferase family protein	[more]
AT1G51990.2	3.4e-54	36.19	O-methyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001077	O-methyltransferase domain	PFAM	PF00891	Methyltransf_2	coord: 149..351 e-value: 3.9E-43 score: 147.2
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 12..123 e-value: 1.2E-24 score: 88.0
IPR016461	O-methyltransferase COMT-type	PIRSF	PIRSF005739	O-mtase	coord: 9..369 e-value: 9.2E-107 score: 355.0
IPR016461	O-methyltransferase COMT-type	PANTHER	PTHR11746	O-METHYLTRANSFERASE	coord: 19..368
IPR016461	O-methyltransferase COMT-type	PROSITE	PS51683	SAM_OMT_II	coord: 31..369 score: 59.120846
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 124..369 e-value: 6.0E-71 score: 240.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..20
None	No IPR available	PANTHER	PTHR11746:SF90	OS02G0823400 PROTEIN	coord: 19..368
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 22..121
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 123..367

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmaCh06G004150	CmaCh06G004150	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh06G004150.1:exon:2063	CmaCh06G004150.1:exon:2063	exon
CmaCh06G004150.1:exon:2062	CmaCh06G004150.1:exon:2062	exon
CmaCh06G004150.1:exon:2061	CmaCh06G004150.1:exon:2061	exon
CmaCh06G004150.1:exon:2060	CmaCh06G004150.1:exon:2060	exon

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh06G004150.1:three_prime_utr	CmaCh06G004150.1:three_prime_utr	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh06G004150.1:cds	CmaCh06G004150.1:cds_4	CDS
CmaCh06G004150.1:cds	CmaCh06G004150.1:cds_3	CDS
CmaCh06G004150.1:cds	CmaCh06G004150.1:cds_2	CDS
CmaCh06G004150.1:cds	CmaCh06G004150.1:cds	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh06G004150.1:five_prime_utr	CmaCh06G004150.1:five_prime_utr	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmaCh06G004150.1	CmaCh06G004150.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019438	aromatic compound biosynthetic process
biological_process	GO:0032259	methylation
biological_process	GO:1901847	nicotinate metabolic process
cellular_component	GO:0005829	cytosol
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0008171	O-methyltransferase activity
molecular_function	GO:0008938	nicotinate N-methyltransferase activity
molecular_function	GO:0046983	protein dimerization activity