CmaCh04G009410.1 (mRNA) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh04G009410.1
Type	mRNA
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Thiamine thiazole synthase, chloroplastic
Location	Cma_Chr04: 4842460 .. 4844391 (+)
Sequence length	1588
RNA-Seq Expression	CmaCh04G009410.1
Synteny	CmaCh04G009410.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGTTTGGAGAACCCCATCTCCCCATTCTGAGCCCTTCATTTTCTATCCTCTCCCCCACATTTCCCCATCCCTTAGATATTTTTCTCACTCAACCAATTTGGGGTAAAATTGAATGCNCCATTTCTCTCTATAAAGCCCTTCTTCCCCCCCAACGCCCTTCCCCATCCCATCTCCTCTGTTTTCCTCTGCATCTACAAAAATGGCTTCCATGGCTTCCACTTTCACCACCAAGCTCCAAACCCCCTCCTCCTCCTCCTCCTCCTTACTCGACTCCTCTTTCCATGGCACCCCTCTGGCCCCTCCTCCTCCTCCTCCTTCTCTCCGCCTCAAATCCACCCCCGCCGCTTTCTCCATCTCCGCCTCCGCCTCTCAGCACCAGCCCTACGATTTGAACTCCTTCAAATTCAACCCCATCAAGGAGTCCATCGTCTCACGTGAGATGACCCGCCGTTACATGACCGACATGATCACTTACGCCGACACTGATGTCGTCATCGTCGGCGCTGGCTCTGCCGGCCTCTCTTGCGCTTATGAACTTAGCAAAAACCCTGACATTCGCATCGCCATCATCGAGCAGTCCGTATCCCCCGGCGGCGGTGCATGGCTCGGTGGCCAGCTCTTCTCTGCTATGGTATTCTTTTAATTCCCTTATATGCCATTTATTTGCCTCACCCTTACCCATAACGCCTCCTTTTCTTTCCTGTCTTTTAGCCTTGAATGCTCTGTTTTTTGTTCTTTGTAAGAAAATACCCACAATTAGATCAACATACTAATTCAGTCATAAACTGGCTTAAGCCCAAGAATTTAAATACTCAATCAGTACAAGATCTGATTCTAAAATCATTAATCTGATGGGTTTGGGCCAATTTAATGTTTGATTCATATTCTTTGATAGGTGGTACGGAAGCCAGCCCACTGCTTCTTGGACGAGGTTGGTGTGGAGTACGACGAGCAAGAGGACTACGTTGTAATTAAGCACGCGGCTCTGTTCACCTCAACGATCATGAGCAAGCTGCTGGCACGGCCCAATGTCAAGCTGTTCAACGCCGTGGCAGCAGAGGATTTGATCGTCAAGGGCGGCAGAGTTGGCGGTGTTGTCACCAACTGGGCTTTAGTGTCAATGAACCATGACACACAGTCCTGCATGGACCCTAATGTCATGGAGGCGAAGGTGGTGGTCAGCTCTTGTGGCCACGACGGGCCCTTCGGAGCCACCGGAGTGAAGCGGCTTAAGAGCATTGGTATGATCGATTCCGTCCCGGGAATGAAGGCCCTGGACATGAACACGGCGGAGGATGCCATTGTTAGGCTGACCCGAGAGGTGGTGCCTGGCATGATCGTAACGGGTATGGAGGTGGCGGAGATCGACGGAGCTCCAAGAATGGTAAAGAACTATGTTTAAATTAGTGGTTTAACTAAAAAGCTTAGACCTTTTTTAACAATCAGAGGTGGTGTTGGATGCAGGGGCCAACGTTCGGGGCAATGATGATATCGGGGCAGAAGGCGGCGCACCTGGCGTTGAAGTCATTGGGGCTGGAAAATTGCATTGAGGATGAAGCAGCTGATCTGATACTGGCGGCGGGAGAGTCGCCGGAGGTTGCAGATGGTTGAAGGGTGAGTTTTCATGGATGGAAGGCGGCGGTTGTGTTTTGTGATTCATGGTGGCTCTGTTTCCGTGGCAAACAGATTGGGGTTTGGGGAAAAGCCAATTTTAAGTTAAATTAAATAAAATTGTATCAAAATGTTAATTGCCCTTTTTTCCTCCCTAATTTAAAGTTAAATTAAATAATTAATCTGGAATTAGATATAATTAGTTGGAAGTTATATTAAAATTAAAATTCTTTGGGTTTATGTATTAAAATCCCGGGAAAGAGTCATTCGACTTCCTGTCTATTTTTTTTTTTTATAAATATTGTGAAAATTAAAACCAAA

mRNA sequence

GGTTTGGAGAACCCCATCTCCCCATTCTGAGCCCTTCATTTTCTATCCTCTCCCCCACATTTCCCCATCCCTTAGATATTTTTCTCACTCAACCAATTTGGGGTAAAATTGAATGCNCCATTTCTCTCTATAAAGCCCTTCTTCCCCCCCAACGCCCTTCCCCATCCCATCTCCTCTGTTTTCCTCTGCATCTACAAAAATGGCTTCCATGGCTTCCACTTTCACCACCAAGCTCCAAACCCCCTCCTCCTCCTCCTCCTCCTTACTCGACTCCTCTTTCCATGGCACCCCTCTGGCCCCTCCTCCTCCTCCTCCTTCTCTCCGCCTCAAATCCACCCCCGCCGCTTTCTCCATCTCCGCCTCCGCCTCTCAGCACCAGCCCTACGATTTGAACTCCTTCAAATTCAACCCCATCAAGGAGTCCATCGTCTCACGTGAGATGACCCGCCGTTACATGACCGACATGATCACTTACGCCGACACTGATGTCGTCATCGTCGGCGCTGGCTCTGCCGGCCTCTCTTGCGCTTATGAACTTAGCAAAAACCCTGACATTCGCATCGCCATCATCGAGCAGTCCGTATCCCCCGGCGGCGGTGCATGGCTCGGTGGCCAGCTCTTCTCTGCTATGGTGGTACGGAAGCCAGCCCACTGCTTCTTGGACGAGGTTGGTGTGGAGTACGACGAGCAAGAGGACTACGTTGTAATTAAGCACGCGGCTCTGTTCACCTCAACGATCATGAGCAAGCTGCTGGCACGGCCCAATGTCAAGCTGTTCAACGCCGTGGCAGCAGAGGATTTGATCGTCAAGGGCGGCAGAGTTGGCGGTGTTGTCACCAACTGGGCTTTAGTGTCAATGAACCATGACACACAGTCCTGCATGGACCCTAATGTCATGGAGGCGAAGGTGGTGGTCAGCTCTTGTGGCCACGACGGGCCCTTCGGAGCCACCGGAGTGAAGCGGCTTAAGAGCATTGGTATGATCGATTCCGTCCCGGGAATGAAGGCCCTGGACATGAACACGGCGGAGGATGCCATTGTTAGGCTGACCCGAGAGGTGGTGCCTGGCATGATCGTAACGGGTATGGAGGTGGCGGAGATCGACGGAGCTCCAAGAATGGGGCCAACGTTCGGGGCAATGATGATATCGGGGCAGAAGGCGGCGCACCTGGCGTTGAAGTCATTGGGGCTGGAAAATTGCATTGAGGATGAAGCAGCTGATCTGATACTGGCGGCGGGAGAGTCGCCGGAGGTTGCAGATGGTTGAAGGGTGAGTTTTCATGGATGGAAGGCGGCGGTTGTGTTTTGTGATTCATGGTGGCTCTGTTTCCGTGGCAAACAGATTGGGGTTTGGGGAAAAGCCAATTTTAAGTTAAATTAAATAAAATTGTATCAAAATGTTAATTGCCCTTTTTTCCTCCCTAATTTAAAGTTAAATTAAATAATTAATCTGGAATTAGATATAATTAGTTGGAAGTTATATTAAAATTAAAATTCTTTGGGTTTATGTATTAAAATCCCGGGAAAGAGTCATTCGACTTCCTGTCTATTTTTTTTTTTTATAAATATTGTGAAAATTAAAACCAAA

Coding sequence (CDS)

ATGGCTTCCATGGCTTCCACTTTCACCACCAAGCTCCAAACCCCCTCCTCCTCCTCCTCCTCCTTACTCGACTCCTCTTTCCATGGCACCCCTCTGGCCCCTCCTCCTCCTCCTCCTTCTCTCCGCCTCAAATCCACCCCCGCCGCTTTCTCCATCTCCGCCTCCGCCTCTCAGCACCAGCCCTACGATTTGAACTCCTTCAAATTCAACCCCATCAAGGAGTCCATCGTCTCACGTGAGATGACCCGCCGTTACATGACCGACATGATCACTTACGCCGACACTGATGTCGTCATCGTCGGCGCTGGCTCTGCCGGCCTCTCTTGCGCTTATGAACTTAGCAAAAACCCTGACATTCGCATCGCCATCATCGAGCAGTCCGTATCCCCCGGCGGCGGTGCATGGCTCGGTGGCCAGCTCTTCTCTGCTATGGTGGTACGGAAGCCAGCCCACTGCTTCTTGGACGAGGTTGGTGTGGAGTACGACGAGCAAGAGGACTACGTTGTAATTAAGCACGCGGCTCTGTTCACCTCAACGATCATGAGCAAGCTGCTGGCACGGCCCAATGTCAAGCTGTTCAACGCCGTGGCAGCAGAGGATTTGATCGTCAAGGGCGGCAGAGTTGGCGGTGTTGTCACCAACTGGGCTTTAGTGTCAATGAACCATGACACACAGTCCTGCATGGACCCTAATGTCATGGAGGCGAAGGTGGTGGTCAGCTCTTGTGGCCACGACGGGCCCTTCGGAGCCACCGGAGTGAAGCGGCTTAAGAGCATTGGTATGATCGATTCCGTCCCGGGAATGAAGGCCCTGGACATGAACACGGCGGAGGATGCCATTGTTAGGCTGACCCGAGAGGTGGTGCCTGGCATGATCGTAACGGGTATGGAGGTGGCGGAGATCGACGGAGCTCCAAGAATGGGGCCAACGTTCGGGGCAATGATGATATCGGGGCAGAAGGCGGCGCACCTGGCGTTGAAGTCATTGGGGCTGGAAAATTGCATTGAGGATGAAGCAGCTGATCTGATACTGGCGGCGGGAGAGTCGCCGGAGGTTGCAGATGGTTGA

Protein sequence

MASMASTFTTKLQTPSSSSSSLLDSSFHGTPLAPPPPPPSLRLKSTPAAFSISASASQHQPYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALKSLGLENCIEDEAADLILAAGESPEVADG

Homology

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BLAST of CmaCh04G009410.1 vs. ExPASy Swiss-Prot
Match: O23787 (Thiamine thiazole synthase, chloroplastic OS=Citrus sinensis OX=2711 GN=THI1 PE=2 SV=1)

HSP 1 Score: 548.9 bits (1413), Expect = 4.2e-155
Identity = 295/363 (81.27%), Postives = 319/363 (87.88%), Query Frame = 0


Query: 1   MASMASTFTTKLQTPSSSSSSLLDSSFHGTPLAPPPPPPSLRLK----STPAAFSISASA 60
           MA+MAS  T    + SS+++ L DSSFHG P++    P  LRL+    S P   SISASA
Sbjct: 1   MAAMAS--TAFAPSVSSTTNKLFDSSFHGAPMS----PSLLRLQPIKSSRPNNLSISASA 60

Query: 61  SQHQPYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKN 120
           S   PYDLN+FKF+PIKESIVSREMTRRYMTDMITYADTDVV+VGAGSAGLSCAYELSKN
Sbjct: 61  S--PPYDLNTFKFDPIKESIVSREMTRRYMTDMITYADTDVVVVGAGSAGLSCAYELSKN 120

Query: 121 PDIRIAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALF 180
           P+I+IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+G++YDEQ++YVVIKHAALF
Sbjct: 121 PNIQIAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHIFLDELGIDYDEQDNYVVIKHAALF 180

Query: 181 TSTIMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAK 240
           TSTIMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAK
Sbjct: 181 TSTIMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAK 240

Query: 241 VVVSSCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGM 300
           VVVSSCGHDGPFGATGVKRLKSIGMI+ VPGMKALDMN+AEDAIVRLTREVVPGMIVTGM
Sbjct: 241 VVVSSCGHDGPFGATGVKRLKSIGMIEEVPGMKALDMNSAEDAIVRLTREVVPGMIVTGM 300

Query: 301 EVAEIDGAPRMGPTFGAMMISGQKAAHLALKSLGLENCIEDEAA-----DLILAAGESPE 355
           EVAEIDGAPRMGPTFGAMMISGQKAAHLALKSLG  N ++         +LILAA +S E
Sbjct: 301 EVAEIDGAPRMGPTFGAMMISGQKAAHLALKSLGQPNALDGTYVGGVHPELILAAADSAE 355

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BLAST of CmaCh04G009410.1 vs. ExPASy Swiss-Prot
Match: Q38709 (Thiamine thiazole synthase, chloroplastic OS=Alnus glutinosa OX=3517 GN=THI1 PE=2 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 7.7e-149
Identity = 281/358 (78.49%), Postives = 309/358 (86.31%), Query Frame = 0


Query: 4   MASTFTTKLQTPSSSSSSLLDSSFHGTPLAPPPPPPSLRLKSTPAAF--SISASASQHQP 63
           MAST T+K Q  +   +S   SSF+GTPLA    P S+R++ T A    SIS S +   P
Sbjct: 1   MASTLTSKPQRLALFENSA--SSFYGTPLA----PSSIRVQPTKAGAKPSISMSGAPSPP 60

Query: 64  YDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRI 123
           YDL +F F+PIKESIVSREMTRRYM DMITYADTDVV+VGAGS+GL C YELSKNP +++
Sbjct: 61  YDLKAFTFDPIKESIVSREMTRRYMMDMITYADTDVVVVGAGSSGLVC-YELSKNPSVQV 120

Query: 124 AIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIM 183
           AIIEQSVSPGGGAWLGGQLFS MVVRKPAH FLDE+G+EYDEQ++YVVIKHAALFTSTIM
Sbjct: 121 AIIEQSVSPGGGAWLGGQLFSGMVVRKPAHLFLDELGIEYDEQDNYVVIKHAALFTSTIM 180

Query: 184 SKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSS 243
           SKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSS
Sbjct: 181 SKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSS 240

Query: 244 CGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEI 303
           CGHDGPFGATGVK L+SIGMID+VPGMKALDMN AEDAIVRLTRE+VPGMIVTGMEVAEI
Sbjct: 241 CGHDGPFGATGVKSLRSIGMIDTVPGMKALDMNVAEDAIVRLTREIVPGMIVTGMEVAEI 300

Query: 304 DGAPRMGPTFGAMMISGQKAAHLALKSLGLENCIEDEAA-----DLILAAGESPEVAD 355
           DGAPRMGPTFGAMMISGQKAAHLALK+LGL N ++         +LILAA +S E+AD
Sbjct: 301 DGAPRMGPTFGAMMISGQKAAHLALKALGLPNALDGSYVGGIHPELILAAADSAEIAD 351

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BLAST of CmaCh04G009410.1 vs. ExPASy Swiss-Prot
Match: F6H7K5 (Thiamine thiazole synthase 2, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-2 PE=3 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 4.2e-147
Identity = 282/361 (78.12%), Postives = 303/361 (83.93%), Query Frame = 0


Query: 1   MASMASTFTTKLQTPSSSSSSLLDSSFHGTPLAPPPPPPSLRLKSTPAAFSISASASQHQ 60
           MASMA+T T+    P  +      SSFHG+P++    P  +  +S       + S S   
Sbjct: 1   MASMATTLTSLSSNPKPAFFD-NKSSFHGSPISYRVLPIKVSHQSP------TISMSSVS 60

Query: 61  PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR 120
           PYDL SFKF PIKESIV+REMTRRYM DMITYADTDVVIVGAGSAGLSCAYELSKNP IR
Sbjct: 61  PYDLQSFKFEPIKESIVAREMTRRYMMDMITYADTDVVIVGAGSAGLSCAYELSKNPSIR 120

Query: 121 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI 180
           +AIIEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+G+EYDEQ++YVVIKHAALFTSTI
Sbjct: 121 VAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHHFLDELGIEYDEQDNYVVIKHAALFTSTI 180

Query: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240
           MSKLLARPNVKLFNAVAAEDLIVK  RV GVVTNWALVSMNHDTQSCMDPNVMEAKVVVS
Sbjct: 181 MSKLLARPNVKLFNAVAAEDLIVKEERVAGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240

Query: 241 SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE 300
           SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTRE+VPGMIVTGMEVAE
Sbjct: 241 SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREIVPGMIVTGMEVAE 300

Query: 301 IDGAPRMGPTFGAMMISGQKAAHLALKSLGLENCI-------EDEAADLILAAGESPEVA 355
           IDGAPRMGPTFGAMMISGQKAAHLAL++LG  N I       E    +LILAA E+ E+ 
Sbjct: 301 IDGAPRMGPTFGAMMISGQKAAHLALRALGQPNAIDGNYTEAETMQPELILAAAETGEIV 354

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BLAST of CmaCh04G009410.1 vs. ExPASy Swiss-Prot
Match: F6H9A9 (Thiamine thiazole synthase 1, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-1 PE=3 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 2.7e-146
Identity = 276/360 (76.67%), Postives = 310/360 (86.11%), Query Frame = 0


Query: 1   MASMASTFTTKLQTPSSSSSSLLDSSFHGTPLAPPPPPPSLRLKSTPAAFSISASASQHQ 60
           MA++ S+  +K   P +S      SSFHG P+A         +KSTP   +++A+A    
Sbjct: 1   MATLTSSICSK---PKASVFDPHKSSFHGVPIATQARLSP--VKSTPVNLAVTAAA---M 60

Query: 61  PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR 120
           PYDL SFKF PIKESIVSREMTRRYM DMITYADTDVV+VGAGSAGLSCAYELSKNP ++
Sbjct: 61  PYDLRSFKFEPIKESIVSREMTRRYMMDMITYADTDVVVVGAGSAGLSCAYELSKNPSVQ 120

Query: 121 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI 180
           +AIIEQSVSPGGGAWLGGQLFS+MVVRKPAH FLDE+G+EYDEQ++YVVIKHAALFTSTI
Sbjct: 121 VAIIEQSVSPGGGAWLGGQLFSSMVVRKPAHRFLDELGLEYDEQDNYVVIKHAALFTSTI 180

Query: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240
           MSKLLARPNVKLFNAVAAEDLI+K G+VGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS
Sbjct: 181 MSKLLARPNVKLFNAVAAEDLIIKEGKVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240

Query: 241 SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE 300
           SCGHDGPFGATGVKRL+S+GMIDSVPGMKALDMNTAED IVRLTREVVPGMIVTGMEVAE
Sbjct: 241 SCGHDGPFGATGVKRLRSVGMIDSVPGMKALDMNTAEDEIVRLTREVVPGMIVTGMEVAE 300

Query: 301 IDGAPRMGPTFGAMMISGQKAAHLALKSLGLENCIE-----DEAADLIL-AAGESPEVAD 355
           IDG+PRMGPTFGAMMISGQKAAHLALKSLGL N ++     +   +L+L AA ++ E+A+
Sbjct: 301 IDGSPRMGPTFGAMMISGQKAAHLALKSLGLPNALDGTYIGNLHPELVLAAAADAAEIAE 352

[Close]

BLAST of CmaCh04G009410.1 vs. ExPASy Swiss-Prot
Match: C5X2M4 (Thiamine thiazole synthase 2, chloroplastic OS=Sorghum bicolor OX=4558 GN=THI1-2 PE=3 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 1.4e-145
Identity = 267/330 (80.91%), Postives = 288/330 (87.27%), Query Frame = 0


Query: 16  SSSSSSLLDSSFHGTPLAPPPPPPSLRLKSTPA---------AFSISASASQHQPYDLNS 75
           ++++SSLL SSF G  L     P S R  S+PA         A   S+ +S + PYDL S
Sbjct: 2   ATTASSLLKSSFAGARL-----PASTRTPSSPAVVATPRGAGAIRASSISSSNPPYDLTS 61

Query: 76  FKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIAIIEQ 135
           F+F+PIKES+VSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSK+P + IAI+EQ
Sbjct: 62  FRFSPIKESVVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKDPSVSIAIVEQ 121

Query: 136 SVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKLLA 195
           SVSPGGGAWLGGQLFSAMVVRKPAH FLDE+GV YDE EDYVVIKHAALFTST+MS+LLA
Sbjct: 122 SVSPGGGAWLGGQLFSAMVVRKPAHLFLDELGVAYDEAEDYVVIKHAALFTSTVMSRLLA 181

Query: 196 RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 255
           RPNVKLFNAVA EDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG
Sbjct: 182 RPNVKLFNAVAVEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 241

Query: 256 PFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPR 315
           PFGATGVKRL+ IGMI  VPGMKALDMNTAED IVRLTREVVPGMIVTGMEVAEIDGAPR
Sbjct: 242 PFGATGVKRLQDIGMISDVPGMKALDMNTAEDEIVRLTREVVPGMIVTGMEVAEIDGAPR 301

Query: 316 MGPTFGAMMISGQKAAHLALKSLGLENCIE 337
           MGPTFGAMMISGQKAAHLALK+LG  N ++
Sbjct: 302 MGPTFGAMMISGQKAAHLALKALGRPNAVD 326

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BLAST of CmaCh04G009410.1 vs. ExPASy TrEMBL
Match: A0A6J1JNT3 (Thiamine thiazole synthase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111487533 PE=3 SV=1)

HSP 1 Score: 690.6 bits (1781), Expect = 3.3e-195
Identity = 355/355 (100.00%), Postives = 355/355 (100.00%), Query Frame = 0


Query: 1   MASMASTFTTKLQTPSSSSSSLLDSSFHGTPLAPPPPPPSLRLKSTPAAFSISASASQHQ 60
           MASMASTFTTKLQTPSSSSSSLLDSSFHGTPLAPPPPPPSLRLKSTPAAFSISASASQHQ
Sbjct: 1   MASMASTFTTKLQTPSSSSSSLLDSSFHGTPLAPPPPPPSLRLKSTPAAFSISASASQHQ 60

Query: 61  PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR 120
           PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR
Sbjct: 61  PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR 120

Query: 121 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI 180
           IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI
Sbjct: 121 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI 180

Query: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240
           MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS
Sbjct: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240

Query: 241 SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE 300
           SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE
Sbjct: 241 SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE 300

Query: 301 IDGAPRMGPTFGAMMISGQKAAHLALKSLGLENCIEDEAADLILAAGESPEVADG 356
           IDGAPRMGPTFGAMMISGQKAAHLALKSLGLENCIEDEAADLILAAGESPEVADG
Sbjct: 301 IDGAPRMGPTFGAMMISGQKAAHLALKSLGLENCIEDEAADLILAAGESPEVADG 355

[Close]

BLAST of CmaCh04G009410.1 vs. ExPASy TrEMBL
Match: A0A6J1FWL6 (Thiamine thiazole synthase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111448035 PE=3 SV=1)

HSP 1 Score: 655.2 bits (1689), Expect = 1.5e-184
Identity = 344/355 (96.90%), Postives = 345/355 (97.18%), Query Frame = 0


Query: 1   MASMASTFTTKLQTPSSSSSSLLDSSFHGTPLAPPPPPPSLRLKSTPAAFSISASASQHQ 60
           MAS+AST TTKLQTP   SSSLLDSSFHGTPLA  PPPPSLRLKSTPAAFSI  SASQ Q
Sbjct: 1   MASVASTLTTKLQTP---SSSLLDSSFHGTPLA--PPPPSLRLKSTPAAFSI--SASQQQ 60

Query: 61  PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR 120
           PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR
Sbjct: 61  PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR 120

Query: 121 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI 180
           IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI
Sbjct: 121 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI 180

Query: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240
           MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS
Sbjct: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240

Query: 241 SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE 300
           SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE
Sbjct: 241 SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE 300

Query: 301 IDGAPRMGPTFGAMMISGQKAAHLALKSLGLENCIEDEAADLILAAGESPEVADG 356
           IDGAPRMGPTFGAMMISGQKAAHLALKSLGL NCIEDEAADLILAAGESPEVADG
Sbjct: 301 IDGAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAADLILAAGESPEVADG 348

[Close]

BLAST of CmaCh04G009410.1 vs. ExPASy TrEMBL
Match: A0A0A0L546 (Thiamine thiazole synthase, chloroplastic OS=Cucumis sativus OX=3659 GN=THI1 PE=3 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 1.0e-164
Identity = 320/366 (87.43%), Postives = 328/366 (89.62%), Query Frame = 0


Query: 1   MASMASTFTTKLQTPSSSSSSLLDSSFHGTPLAPPPPPPSLRLKSTPA-AFSISASASQH 60
           MAS+AST TTKLQ P    S L DSSFHGTPLA    P SLRLKST A + +ISASASQ 
Sbjct: 1   MASIASTLTTKLQKP----SLLHDSSFHGTPLA---SPSSLRLKSTAATSLTISASASQ- 60

Query: 61  QPYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDI 120
            PYDLN FKFNPI+ESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNP+I
Sbjct: 61  PPYDLNQFKFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNI 120

Query: 121 RIAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTST 180
           RIAIIEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDEVGVEYDEQ+DYVVIKHAALFTST
Sbjct: 121 RIAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTST 180

Query: 181 IMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVV 240
           IMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVV
Sbjct: 181 IMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVV 240

Query: 241 SSCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVA 300
           SSCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVA
Sbjct: 241 SSCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVA 300

Query: 301 EIDGAPRMGPTFGAMMISGQKAAHLALKSLGLENCIEDEA-----------ADLILAAGE 355
           EIDGAPRMGPTFGAMMISGQKAAHLALKSLG  N I DE             +L+LAAGE
Sbjct: 301 EIDGAPRMGPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGE 358

[Close]

BLAST of CmaCh04G009410.1 vs. ExPASy TrEMBL
Match: A0A1S3CJ81 (Thiamine thiazole synthase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501605 PE=3 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 6.7e-164
Identity = 317/366 (86.61%), Postives = 327/366 (89.34%), Query Frame = 0


Query: 1   MASMASTFTTKLQTPSSSSSSLLDSSFHGTPLAPPPPPPSLRLKSTPAAFSISASASQHQ 60
           MAS+AST TTKLQ P    S L DSSFHGTPLA    PPSLRLKS PAA S++ SAS  Q
Sbjct: 1   MASIASTLTTKLQIP----SLLHDSSFHGTPLA---SPPSLRLKS-PAATSLTISASASQ 60

Query: 61  -PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDI 120
            PYDLN FKFNPI+ESIVSREMTRRYMTDMITYADTDV+IVGAGSAGLSCAYELSKNP+I
Sbjct: 61  PPYDLNQFKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNI 120

Query: 121 RIAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTST 180
           RIAIIEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDEVGVEYDEQ+DYVVIKHAALFTST
Sbjct: 121 RIAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTST 180

Query: 181 IMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVV 240
           IMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVV
Sbjct: 181 IMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVV 240

Query: 241 SSCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVA 300
           SSCGHDGPFGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVA
Sbjct: 241 SSCGHDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVA 300

Query: 301 EIDGAPRMGPTFGAMMISGQKAAHLALKSLGLENCIEDEA-----------ADLILAAGE 355
           EIDGAPRMGPTFGAMMISGQKAAHLALKSLG  N I  E             +L+LAAGE
Sbjct: 301 EIDGAPRMGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGE 358

[Close]

BLAST of CmaCh04G009410.1 vs. ExPASy TrEMBL
Match: A0A6J1J7L4 (Thiamine thiazole synthase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111483293 PE=3 SV=1)

HSP 1 Score: 577.4 bits (1487), Expect = 4.1e-161
Identity = 306/364 (84.07%), Postives = 324/364 (89.01%), Query Frame = 0


Query: 1   MASMASTFTTKLQTPSSSSSSLLDSSFHGTPLAPPPPPPSLRLKSTPAAFSISASASQHQ 60
           MAS+AS+  TKL  P     SLLDSSFHG+PL    P PS RLK T AA S+S SAS  Q
Sbjct: 1   MASIASSLPTKLHRP-----SLLDSSFHGSPLL---PTPSFRLKPTTAAASLSISASAAQ 60

Query: 61  -PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDI 120
            PYDLN FKFNPI+ESIVSREMTRRYMTDMITYADTDV++VGAGSAGLSCAYELSK+P +
Sbjct: 61  PPYDLNQFKFNPIRESIVSREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKDPSV 120

Query: 121 RIAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTST 180
           R+AIIEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+ VEYDEQEDYVVIKHAALFTST
Sbjct: 121 RVAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHYFLDELEVEYDEQEDYVVIKHAALFTST 180

Query: 181 IMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVV 240
           IMSKLLARPNVKLFNAVAAEDLIVK GRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVV
Sbjct: 181 IMSKLLARPNVKLFNAVAAEDLIVKEGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVV 240

Query: 241 SSCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVA 300
           SSCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVA
Sbjct: 241 SSCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVA 300

Query: 301 EIDGAPRMGPTFGAMMISGQKAAHLALKSLGLENCIED---------EAADLILAAGESP 355
           EIDGAPRMGPTFGAMMISGQKAAHLALK+LG  N I++         EAA+++LAAGE+P
Sbjct: 301 EIDGAPRMGPTFGAMMISGQKAAHLALKALGKANSIDEKGENGGRKVEAAEMVLAAGETP 356

[Close]

BLAST of CmaCh04G009410.1 vs. NCBI nr
Match: XP_022990741.1 (thiamine thiazole synthase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 690.6 bits (1781), Expect = 6.9e-195
Identity = 355/355 (100.00%), Postives = 355/355 (100.00%), Query Frame = 0


Query: 1   MASMASTFTTKLQTPSSSSSSLLDSSFHGTPLAPPPPPPSLRLKSTPAAFSISASASQHQ 60
           MASMASTFTTKLQTPSSSSSSLLDSSFHGTPLAPPPPPPSLRLKSTPAAFSISASASQHQ
Sbjct: 1   MASMASTFTTKLQTPSSSSSSLLDSSFHGTPLAPPPPPPSLRLKSTPAAFSISASASQHQ 60

Query: 61  PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR 120
           PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR
Sbjct: 61  PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR 120

Query: 121 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI 180
           IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI
Sbjct: 121 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI 180

Query: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240
           MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS
Sbjct: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240

Query: 241 SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE 300
           SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE
Sbjct: 241 SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE 300

Query: 301 IDGAPRMGPTFGAMMISGQKAAHLALKSLGLENCIEDEAADLILAAGESPEVADG 356
           IDGAPRMGPTFGAMMISGQKAAHLALKSLGLENCIEDEAADLILAAGESPEVADG
Sbjct: 301 IDGAPRMGPTFGAMMISGQKAAHLALKSLGLENCIEDEAADLILAAGESPEVADG 355

[Close]

BLAST of CmaCh04G009410.1 vs. NCBI nr
Match: KAG7031400.1 (Thiamine thiazole synthase 2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 667.9 bits (1722), Expect = 4.8e-188
Identity = 347/355 (97.75%), Postives = 348/355 (98.03%), Query Frame = 0


Query: 1   MASMASTFTTKLQTPSSSSSSLLDSSFHGTPLAPPPPPPSLRLKSTPAAFSISASASQHQ 60
           MASMAST TTKLQTPSSSSSSLLDSSFHGTPLA   PPPSLRLK TPA+FSISASASQ Q
Sbjct: 1   MASMASTLTTKLQTPSSSSSSLLDSSFHGTPLA---PPPSLRLKPTPASFSISASASQQQ 60

Query: 61  PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR 120
           PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR
Sbjct: 61  PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR 120

Query: 121 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI 180
           IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI
Sbjct: 121 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI 180

Query: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240
           MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS
Sbjct: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240

Query: 241 SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE 300
           SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE
Sbjct: 241 SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE 300

Query: 301 IDGAPRMGPTFGAMMISGQKAAHLALKSLGLENCIEDEAADLILAAGESPEVADG 356
           IDGAPRMGPTFGAMMISGQKAAHLALKSLGL NCIEDEAADLILAAGESPEVADG
Sbjct: 301 IDGAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAADLILAAGESPEVADG 352

[Close]

BLAST of CmaCh04G009410.1 vs. NCBI nr
Match: XP_023524627.1 (thiamine thiazole synthase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 662.1 bits (1707), Expect = 2.6e-186
Identity = 345/355 (97.18%), Postives = 347/355 (97.75%), Query Frame = 0


Query: 1   MASMASTFTTKLQTPSSSSSSLLDSSFHGTPLAPPPPPPSLRLKSTPAAFSISASASQHQ 60
           MASMAST TTKLQTP  SSSSLLDSSFHGTPLA  PPPPSLRLK TPA+FSISAS+SQ Q
Sbjct: 1   MASMASTLTTKLQTP--SSSSLLDSSFHGTPLA--PPPPSLRLKPTPASFSISASSSQQQ 60

Query: 61  PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR 120
           PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR
Sbjct: 61  PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR 120

Query: 121 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI 180
           IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI
Sbjct: 121 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI 180

Query: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240
           MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS
Sbjct: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240

Query: 241 SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE 300
           SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE
Sbjct: 241 SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE 300

Query: 301 IDGAPRMGPTFGAMMISGQKAAHLALKSLGLENCIEDEAADLILAAGESPEVADG 356
           IDGAPRMGPTFGAMMISGQKAAHLALKSLGL NCIEDEAADLILAAGESPEVADG
Sbjct: 301 IDGAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAADLILAAGESPEVADG 351

[Close]

BLAST of CmaCh04G009410.1 vs. NCBI nr
Match: KAG6600763.1 (Thiamine thiazole synthase 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 661.0 bits (1704), Expect = 5.8e-186
Identity = 346/356 (97.19%), Postives = 347/356 (97.47%), Query Frame = 0


Query: 1   MASMASTFTTKLQTP-SSSSSSLLDSSFHGTPLAPPPPPPSLRLKSTPAAFSISASASQH 60
           MASMAST T KLQTP SSSSSSLLDSSFHGTPLA   PPPSLRLK TPA+FSISASASQ 
Sbjct: 1   MASMASTLTIKLQTPSSSSSSSLLDSSFHGTPLA---PPPSLRLKPTPASFSISASASQQ 60

Query: 61  QPYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDI 120
           QPYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDI
Sbjct: 61  QPYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDI 120

Query: 121 RIAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTST 180
           RIAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTST
Sbjct: 121 RIAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTST 180

Query: 181 IMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVV 240
           IMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVV
Sbjct: 181 IMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVV 240

Query: 241 SSCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVA 300
           SSCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVA
Sbjct: 241 SSCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVA 300

Query: 301 EIDGAPRMGPTFGAMMISGQKAAHLALKSLGLENCIEDEAADLILAAGESPEVADG 356
           EIDGAPRMGPTFGAMMISGQKAAHLALKSLGL NCIEDEAADLILAAGESPEVADG
Sbjct: 301 EIDGAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAADLILAAGESPEVADG 353

[Close]

BLAST of CmaCh04G009410.1 vs. NCBI nr
Match: XP_022943248.1 (thiamine thiazole synthase, chloroplastic [Cucurbita moschata])

HSP 1 Score: 655.2 bits (1689), Expect = 3.2e-184
Identity = 344/355 (96.90%), Postives = 345/355 (97.18%), Query Frame = 0


Query: 1   MASMASTFTTKLQTPSSSSSSLLDSSFHGTPLAPPPPPPSLRLKSTPAAFSISASASQHQ 60
           MAS+AST TTKLQTP   SSSLLDSSFHGTPLA  PPPPSLRLKSTPAAFSI  SASQ Q
Sbjct: 1   MASVASTLTTKLQTP---SSSLLDSSFHGTPLA--PPPPSLRLKSTPAAFSI--SASQQQ 60

Query: 61  PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR 120
           PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR
Sbjct: 61  PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR 120

Query: 121 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI 180
           IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI
Sbjct: 121 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI 180

Query: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240
           MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS
Sbjct: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240

Query: 241 SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE 300
           SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE
Sbjct: 241 SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE 300

Query: 301 IDGAPRMGPTFGAMMISGQKAAHLALKSLGLENCIEDEAADLILAAGESPEVADG 356
           IDGAPRMGPTFGAMMISGQKAAHLALKSLGL NCIEDEAADLILAAGESPEVADG
Sbjct: 301 IDGAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAADLILAAGESPEVADG 348

[Close]

BLAST of CmaCh04G009410.1 vs. TAIR 10
Match: AT5G54770.1 (thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4) )

HSP 1 Score: 516.9 bits (1330), Expect = 1.3e-146
Identity = 274/359 (76.32%), Postives = 307/359 (85.52%), Query Frame = 0


Query: 1   MASMASTFTTKLQTPSSSSSSLLDSSFHGTPLAPPPPPPSLRLKSTPAAFSISASASQHQ 60
           MA++AST    L   S+    L DSSFHG+ ++    P S+ LK  P +FS+ A+ +   
Sbjct: 1   MAAIAST----LSLSSTKPQRLFDSSFHGSAIS--AAPISIGLK--PRSFSVRATTA--- 60

Query: 61  PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR 120
            YDLN+F F+PIKESIVSREMTRRYMTDMITYA+TDVV+VGAGSAGLS AYE+SKNP+++
Sbjct: 61  GYDLNAFTFDPIKESIVSREMTRRYMTDMITYAETDVVVVGAGSAGLSAAYEISKNPNVQ 120

Query: 121 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI 180
           +AIIEQSVSPGGGAWLGGQLFSAM+VRKPAH FLDE+GV YDEQ+ YVV+KHAALFTSTI
Sbjct: 121 VAIIEQSVSPGGGAWLGGQLFSAMIVRKPAHLFLDEIGVAYDEQDTYVVVKHAALFTSTI 180

Query: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240
           MSKLLARPNVKLFNAVAAEDLIVKG RVGGVVTNWALV+ NH TQSCMDPNVMEAK+VVS
Sbjct: 181 MSKLLARPNVKLFNAVAAEDLIVKGNRVGGVVTNWALVAQNHHTQSCMDPNVMEAKIVVS 240

Query: 241 SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE 300
           SCGHDGPFGATGVKRLKSIGMID VPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE
Sbjct: 241 SCGHDGPFGATGVKRLKSIGMIDHVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE 300

Query: 301 IDGAPRMGPTFGAMMISGQKAAHLALKSLGLENCIE-----DEAADLILAAGESPEVAD 355
           IDGAPRMGPTFGAMMISGQKA  LALK+LGL N I+     + + +L+LAA +S E  D
Sbjct: 301 IDGAPRMGPTFGAMMISGQKAGQLALKALGLPNAIDGTLVGNLSPELVLAAADSAETVD 348

The following BLAST results are available for this feature:

BLAST of CmaCh04G009410.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23787	4.2e-155	81.27	Thiamine thiazole synthase, chloroplastic OS=Citrus sinensis OX=2711 GN=THI1 PE=...	[more]
Q38709	7.7e-149	78.49	Thiamine thiazole synthase, chloroplastic OS=Alnus glutinosa OX=3517 GN=THI1 PE=...	[more]
F6H7K5	4.2e-147	78.12	Thiamine thiazole synthase 2, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-2...	[more]
F6H9A9	2.7e-146	76.67	Thiamine thiazole synthase 1, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-1...	[more]
C5X2M4	1.4e-145	80.91	Thiamine thiazole synthase 2, chloroplastic OS=Sorghum bicolor OX=4558 GN=THI1-2...	[more]

BLAST of CmaCh04G009410.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JNT3	3.3e-195	100.00	Thiamine thiazole synthase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1FWL6	1.5e-184	96.90	Thiamine thiazole synthase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A0A0L546	1.0e-164	87.43	Thiamine thiazole synthase, chloroplastic OS=Cucumis sativus OX=3659 GN=THI1 PE=...	[more]
A0A1S3CJ81	6.7e-164	86.61	Thiamine thiazole synthase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC10350160...	[more]
A0A6J1J7L4	4.1e-161	84.07	Thiamine thiazole synthase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]

BLAST of CmaCh04G009410.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022990741.1	6.9e-195	100.00	thiamine thiazole synthase, chloroplastic [Cucurbita maxima]	[more]
KAG7031400.1	4.8e-188	97.75	Thiamine thiazole synthase 2, chloroplastic [Cucurbita argyrosperma subsp. argyr...	[more]
XP_023524627.1	2.6e-186	97.18	thiamine thiazole synthase, chloroplastic [Cucurbita pepo subsp. pepo]	[more]
KAG6600763.1	5.8e-186	97.19	Thiamine thiazole synthase 2, chloroplastic, partial [Cucurbita argyrosperma sub...	[more]
XP_022943248.1	3.2e-184	96.90	thiamine thiazole synthase, chloroplastic [Cucurbita moschata]	[more]

BLAST of CmaCh04G009410.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G54770.1	1.3e-146	76.32	thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4)	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	6.10.250.2840		coord: 63..93 e-value: 2.4E-19 score: 70.6
None	No IPR available	PFAM	PF01946	Thi4	coord: 78..311 e-value: 1.0E-114 score: 381.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..44
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..27
None	No IPR available	PANTHER	PTHR43422	THIAMINE THIAZOLE SYNTHASE	coord: 1..355
None	No IPR available	PANTHER	PTHR43422:SF11	THIAMINE THIAZOLE SYNTHASE, CHLOROPLASTIC	coord: 1..355
IPR002922	Thiazole biosynthetic enzyme Thi4 family	TIGRFAM	TIGR00292	TIGR00292	coord: 74..329 e-value: 7.2E-82 score: 272.4
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 94..329 e-value: 1.6E-122 score: 409.2
IPR036188	FAD/NAD(P)-binding domain superfamily	SUPERFAMILY	51905	FAD/NAD(P)-binding domain	coord: 57..323
IPR027495	Thiamine thiazole synthase	HAMAP	MF_03158	THI4	coord: 5..338 score: 68.17038

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmaCh04G009410	CmaCh04G009410	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh04G009410.1:exon:484	CmaCh04G009410.1:exon:484	exon
CmaCh04G009410.1:exon:485	CmaCh04G009410.1:exon:485	exon
CmaCh04G009410.1:exon:486	CmaCh04G009410.1:exon:486	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh04G009410.1:five_prime_utr	CmaCh04G009410.1:five_prime_utr	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh04G009410.1:cds	CmaCh04G009410.1:cds	CDS
CmaCh04G009410.1:cds	CmaCh04G009410.1:cds_2	CDS
CmaCh04G009410.1:cds	CmaCh04G009410.1:cds_3	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh04G009410.1:three_prime_utr	CmaCh04G009410.1:three_prime_utr	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmaCh04G009410.1	CmaCh04G009410.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009228	thiamine biosynthetic process
biological_process	GO:0016310	phosphorylation
biological_process	GO:0052837	thiazole biosynthetic process
cellular_component	GO:0009570	chloroplast stroma
cellular_component	GO:0005829	cytosol
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016301	kinase activity
molecular_function	GO:0016763	pentosyltransferase activity