CmaCh03G004550.1 (mRNA) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh03G004550.1 vs. ExPASy Swiss-Prot
Match: Q40863 (Embryogenesis-associated protein EMB8 OS=Picea glauca OX=3330 GN=EMB8 PE=2 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 2.6e-133
Identity = 230/355 (64.79%), Postives = 275/355 (77.46%), Query Frame = 0

Query: 89  EVIGGGRDRFLPALKDLNLPYKPFPVIGWNRHLETIFASFYRSCPDVRFRRECLRAADNG 148
           E+IG  R+  +  L  L  PY+ FP +G NRH+ETIFASF+RS P ++ RRECLR  D G
Sbjct: 69  EIIGSDRE-LMSKLTTLGRPYRHFPFMG-NRHVETIFASFFRSWPVIKSRRECLRMEDGG 128

Query: 149 TVALDW-VVGDDL-----RLPIDAPVLILLPGLTGGSQDSYVRHMLVRARDRGWRAVVFN 208
           TV LDW + G+D       LP+++PVLILLPGLTGGS DSYV+HML+RAR  GW +VVFN
Sbjct: 129 TVELDWPLEGEDAELWNGELPVNSPVLILLPGLTGGSDDSYVKHMLLRARKHGWHSVVFN 188

Query: 209 SRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKANVYAVGWSLGGNILVNYLGQDSL 268
           SRGC +SPVTTPQFYSASF  D+ +VV+HV  R+ ++N+YAVGWSLG NILV YLG+ + 
Sbjct: 189 SRGCADSPVTTPQFYSASFTKDLCQVVKHVAVRFSESNIYAVGWSLGANILVRYLGEVAG 248

Query: 269 SCPLSGAVSLCNPFDLVVADEDFRK--GFNNVYDKALARALRRIFSKHADLFNDMPGEFN 328
           +CPLSGAVSLCNPF+LV+ADEDF K  GFNNVYDKALAR LR+IF KH  LF  + GE+N
Sbjct: 249 NCPLSGAVSLCNPFNLVIADEDFHKGLGFNNVYDKALARGLRQIFPKHTRLFEGIEGEYN 308

Query: 329 IPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPD 388
           IP  A AR+VR++D  LTRVSFGF SV +YYS SSSS  IK V+  LLCIQA+NDPIAP 
Sbjct: 309 IPTVAKARSVRDFDGGLTRVSFGFQSVGDYYSNSSSSLSIKYVQTSLLCIQASNDPIAPS 368

Query: 389 RGVPREDIKANPNCLLIVTPKGGHLGWVAGPSAPLGNPWTDPFVIDFLVYLESQE 436
           RG+P EDIK NPNCLL+VTP GGHLGWVAG  AP G PWTDP V+++L  LE  +
Sbjct: 369 RGIPWEDIKENPNCLLVVTPNGGHLGWVAGDDAPFGAPWTDPLVMEYLEVLEKNQ 421

BLAST of CmaCh03G004550.1 vs. ExPASy Swiss-Prot
Match: Q91ZH7 (Phospholipase ABHD3 OS=Mus musculus OX=10090 GN=Abhd3 PE=1 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 3.1e-54
Identity = 112/337 (33.23%), Postives = 180/337 (53.41%), Query Frame = 0

Query: 82  IQPHPSLEVIGGGRDRFL----PALKDLNLPYKPFPVIGWNRHLETIFASFYRSCPDVRF 141
           I   P L + G    RFL    P + +   P     V  W    +T+   F  S P V++
Sbjct: 52  IAKKPQLVIGGESFSRFLQDHCPVVTETYYP----TVWCWESRGQTLLRPFITSKPPVQY 111

Query: 142 RRECLRAADNGTVALDWVVGDDLRLPIDA---PVLILLPGLTGGSQDSYVRHMLVRARDR 201
           R E ++ AD G ++LDW   ++    +DA   P ++LLPGLTG S++SY+ HM+  + + 
Sbjct: 112 RNELIKTADGGQISLDWFDNNNSAYYVDASTRPTILLLPGLTGTSKESYILHMIHLSEEL 171

Query: 202 GWRAVVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKANVYAVGWSLGGNILV 261
           G+R VVFN+RG     + TP+ Y  +   D+  VV HV   YP A   A G S+GG +L+
Sbjct: 172 GYRCVVFNNRGVAGESLLTPRTYCCANTEDLEAVVHHVHSLYPGAPFLAAGVSMGGMLLL 231

Query: 262 NYLGQDSLSCPLSGAVSLCNPFDLVVADEDFRKGFN-NVYDKALARALRRIFSKHADLFN 321
           NYLG+     PL  A +    ++     E   +  N  +++  L   L+    KH  +F 
Sbjct: 232 NYLGKIGSKTPLMAAATFSVGWNTFACSESLERPLNWLLFNYYLTTCLQSSVKKHRHMFV 291

Query: 322 DMPGEFNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAA 381
           +   + ++     A+++RE+D   T V FG+ ++D+YY+ +S ++ +K V IP+LC+ A 
Sbjct: 292 E---QIDMDQVMKAKSIREFDKRFTAVMFGYRTLDDYYTDASPNRRLKSVGIPVLCLNAT 351

Query: 382 NDPIAPDRGVPREDIKANPNCLLIVTPKGGHLGWVAG 411
           +D  +P   +P E  K NPN  L++T  GGH+G++ G
Sbjct: 352 DDVFSPSHAIPIETAKQNPNVALVLTAYGGHIGFLEG 381

BLAST of CmaCh03G004550.1 vs. ExPASy Swiss-Prot
Match: Q0VC00 (Phospholipase ABHD3 OS=Bos taurus OX=9913 GN=ABHD3 PE=2 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 4.1e-54
Identity = 113/337 (33.53%), Postives = 180/337 (53.41%), Query Frame = 0

Query: 82  IQPHPSLEVIGGGRDRFL----PALKDLNLPYKPFPVIGWNRHLETIFASFYRSCPDVRF 141
           I   P L   G    RFL    P + +   P     V  W    +T+   F  S P V++
Sbjct: 52  IAKKPQLVTGGESFSRFLQDHCPVVTETYYP----TVWCWESRGQTLLRPFITSKPLVQY 111

Query: 142 RRECLRAADNGTVALDWVVGDDLRLPIDA---PVLILLPGLTGGSQDSYVRHMLVRARDR 201
           R E ++ AD G ++LDW   D+ +  +DA   P ++LLPGLTG S++SY+ HM+  + + 
Sbjct: 112 RNELIKTADGGQISLDWFDNDNSKHYMDASTRPTVLLLPGLTGTSKESYILHMIHLSEEL 171

Query: 202 GWRAVVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKANVYAVGWSLGGNILV 261
           G+R VVFN+RG     + TP+ Y  S   D+  V+ HV   YP A   A G S+GG +L+
Sbjct: 172 GYRYVVFNNRGVAGENLLTPRTYCCSNTEDLETVIHHVHSLYPSAPFLAAGVSMGGMLLL 231

Query: 262 NYLGQDSLSCPLSGAVSLCNPFDLVVADEDFRKGFN-NVYDKALARALRRIFSKHADLFN 321
           NYLG+     PL  A +    ++     E   K  N  +++  L   L+   +KH  +F 
Sbjct: 232 NYLGKIGPKTPLKAAATFSVGWNTFACSESLEKPLNWLLFNYYLTTCLQSSVNKHRHMF- 291

Query: 322 DMPGEFNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAA 381
               + ++     A+++RE+D   T V FG+ ++D+YY+ +S ++ +K V IP+LC+ + 
Sbjct: 292 --VKQIDVDHVMKAKSIREFDKRFTSVMFGYRTIDDYYTDASPNRRLKSVGIPVLCLNSV 351

Query: 382 NDPIAPDRGVPREDIKANPNCLLIVTPKGGHLGWVAG 411
           +D  +P   +P E  K NPN  L++T  GGH+G++ G
Sbjct: 352 DDVFSPSHAIPIETAKQNPNVALVLTSYGGHIGFLEG 381

BLAST of CmaCh03G004550.1 vs. ExPASy Swiss-Prot
Match: Q8WU67 (Phospholipase ABHD3 OS=Homo sapiens OX=9606 GN=ABHD3 PE=2 SV=2)

HSP 1 Score: 213.0 bits (541), Expect = 7.0e-54
Identity = 113/337 (33.53%), Postives = 178/337 (52.82%), Query Frame = 0

Query: 82  IQPHPSLEVIGGGRDRFL----PALKDLNLPYKPFPVIGWNRHLETIFASFYRSCPDVRF 141
           I   P L   G    RFL    P + +   P     V  W    +T+   F  S P V++
Sbjct: 52  IAKKPQLVTGGESFSRFLQDHCPVVTETYYP----TVWCWEGRGQTLLRPFITSKPPVQY 111

Query: 142 RRECLRAADNGTVALDWVVGDDLRLPIDA---PVLILLPGLTGGSQDSYVRHMLVRARDR 201
           R E ++ AD G ++LDW   D+    +DA   P ++LLPGLTG S++SY+ HM+  + + 
Sbjct: 112 RNELIKTADGGQISLDWFDNDNSTCYMDASTRPTILLLPGLTGTSKESYILHMIHLSEEL 171

Query: 202 GWRAVVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKANVYAVGWSLGGNILV 261
           G+R VVFN+RG     + TP+ Y  +   D+  V+ HV   YP A   A G S+GG +L+
Sbjct: 172 GYRCVVFNNRGVAGENLLTPRTYCCANTEDLETVIHHVHSLYPSAPFLAAGVSMGGMLLL 231

Query: 262 NYLGQDSLSCPLSGAVSLCNPFDLVVADEDFRKGFN-NVYDKALARALRRIFSKHADLFN 321
           NYLG+     PL  A +    ++     E   K  N  +++  L   L+   +KH  +F 
Sbjct: 232 NYLGKIGSKTPLMAAATFSVGWNTFACSESLEKPLNWLLFNYYLTTCLQSSVNKHRHMF- 291

Query: 322 DMPGEFNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAA 381
               + ++     A+++RE+D   T V FG+ ++D+YY+ +S S  +K V IP+LC+ + 
Sbjct: 292 --VKQVDMDHVMKAKSIREFDKRFTSVMFGYQTIDDYYTDASPSPRLKSVGIPVLCLNSV 351

Query: 382 NDPIAPDRGVPREDIKANPNCLLIVTPKGGHLGWVAG 411
           +D  +P   +P E  K NPN  L++T  GGH+G++ G
Sbjct: 352 DDVFSPSHAIPIETAKQNPNVALVLTSYGGHIGFLEG 381

BLAST of CmaCh03G004550.1 vs. ExPASy Swiss-Prot
Match: Q54H38 (Abhydrolase domain-containing protein OS=Dictyostelium discoideum OX=44689 GN=abhd PE=1 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 1.8e-49
Identity = 117/335 (34.93%), Postives = 188/335 (56.12%), Query Frame = 0

Query: 100 PALKDLNLPYKPFPVIGWNRHLETIFASFYRSCPDVRFRRECL-RAADNGTVALDWVVGD 159
           P +K+    Y P+ +  +N H    + S+     +++ RRE L    D GT++LD+    
Sbjct: 53  PTIKNGVTFYPPYYL--YNSHFMNYYGSYKIPKLNLKTRREILVNPIDGGTISLDFFELG 112

Query: 160 DLRLPIDAPVLILLPGLTGGSQDSYVRHMLVRA-RDRGWRAVVFNSRGCGNSPVTTPQFY 219
           + +   D P +++  GLTGGS + YV++   RA +++G+R+VVFN RGC  +P+T  + Y
Sbjct: 113 EFK--EDTPTIVINHGLTGGSHERYVQYFAQRAYKEKGFRSVVFNYRGCAGNPITADRAY 172

Query: 220 SASFLGDMREVVEHVTK-RYPKANV-YAVGWSLGGNILVNYLGQDSLSCPLSGAVSLCNP 279
           SA  L D++ V E++TK   P     + VG+SLG  ILVNY+       P    VS+ NP
Sbjct: 173 SAVQLDDIKFVTEYLTKTALPLVKKWFLVGFSLGSAILVNYMADAGKDSPYLAHVSISNP 232

Query: 280 FDLVVADEDFRKGF--NNVYDKALARALRRIFSKHADLFNDMPGEFNIPLAANARTVREY 339
            ++V   ++    +  N +Y+K LA  L+R+F K     +    +  I     A+T+ ++
Sbjct: 233 MNMVECTKNLSSTYINNLIYNKGLANNLKRLFRKFDGRLDKYATKEQI---MAAQTIADF 292

Query: 340 DDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPDRGVPREDIKANPN 399
           DD +T   FGF +  +YY  +SSSK I+++  P+L I A +DPIAP  G P +D K+NPN
Sbjct: 293 DDLITSKMFGFETAHDYYLAASSSKSIRNLVKPILFINAIDDPIAPTSGFPWKDFKSNPN 352

Query: 400 CLLIVTPKGGHLGWVAGPSAPLGNPWTDPFVIDFL 429
            +L V+  GGHLG++   S      W+D   +++L
Sbjct: 353 TILCVSRWGGHLGFI---SYEDHMSWSDKAAVEYL 377

BLAST of CmaCh03G004550.1 vs. ExPASy TrEMBL
Match: A0A6J1IJD9 (embryogenesis-associated protein EMB8-like OS=Cucurbita maxima OX=3661 GN=LOC111477973 PE=4 SV=1)

HSP 1 Score: 926.4 bits (2393), Expect = 4.6e-266
Identity = 452/452 (100.00%), Postives = 452/452 (100.00%), Query Frame = 0

Query: 1   MACAGPIIITAVPHRHIQTPIPFHFPPMARAFLGSTPSSSLLTTSRLSSFTPSLITNQRR 60
           MACAGPIIITAVPHRHIQTPIPFHFPPMARAFLGSTPSSSLLTTSRLSSFTPSLITNQRR
Sbjct: 1   MACAGPIIITAVPHRHIQTPIPFHFPPMARAFLGSTPSSSLLTTSRLSSFTPSLITNQRR 60

Query: 61  RSLRLTTAAVVAMSEFPSEKPIQPHPSLEVIGGGRDRFLPALKDLNLPYKPFPVIGWNRH 120
           RSLRLTTAAVVAMSEFPSEKPIQPHPSLEVIGGGRDRFLPALKDLNLPYKPFPVIGWNRH
Sbjct: 61  RSLRLTTAAVVAMSEFPSEKPIQPHPSLEVIGGGRDRFLPALKDLNLPYKPFPVIGWNRH 120

Query: 121 LETIFASFYRSCPDVRFRRECLRAADNGTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQ 180
           LETIFASFYRSCPDVRFRRECLRAADNGTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQ
Sbjct: 121 LETIFASFYRSCPDVRFRRECLRAADNGTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQ 180

Query: 181 DSYVRHMLVRARDRGWRAVVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKAN 240
           DSYVRHMLVRARDRGWRAVVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKAN
Sbjct: 181 DSYVRHMLVRARDRGWRAVVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKAN 240

Query: 241 VYAVGWSLGGNILVNYLGQDSLSCPLSGAVSLCNPFDLVVADEDFRKGFNNVYDKALARA 300
           VYAVGWSLGGNILVNYLGQDSLSCPLSGAVSLCNPFDLVVADEDFRKGFNNVYDKALARA
Sbjct: 241 VYAVGWSLGGNILVNYLGQDSLSCPLSGAVSLCNPFDLVVADEDFRKGFNNVYDKALARA 300

Query: 301 LRRIFSKHADLFNDMPGEFNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFI 360
           LRRIFSKHADLFNDMPGEFNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFI
Sbjct: 301 LRRIFSKHADLFNDMPGEFNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFI 360

Query: 361 KDVKIPLLCIQAANDPIAPDRGVPREDIKANPNCLLIVTPKGGHLGWVAGPSAPLGNPWT 420
           KDVKIPLLCIQAANDPIAPDRGVPREDIKANPNCLLIVTPKGGHLGWVAGPSAPLGNPWT
Sbjct: 361 KDVKIPLLCIQAANDPIAPDRGVPREDIKANPNCLLIVTPKGGHLGWVAGPSAPLGNPWT 420

Query: 421 DPFVIDFLVYLESQESKASASSTDLTAAETLV 453
           DPFVIDFLVYLESQESKASASSTDLTAAETLV
Sbjct: 421 DPFVIDFLVYLESQESKASASSTDLTAAETLV 452

BLAST of CmaCh03G004550.1 vs. ExPASy TrEMBL
Match: A0A6J1GD89 (embryogenesis-associated protein EMB8-like OS=Cucurbita moschata OX=3662 GN=LOC111453123 PE=4 SV=1)

HSP 1 Score: 919.5 bits (2375), Expect = 5.6e-264
Identity = 449/452 (99.34%), Postives = 449/452 (99.34%), Query Frame = 0

Query: 1   MACAGPIIITAVPHRHIQTPIPFHFPPMARAFLGSTPSSSLLTTSRLSSFTPSLITNQRR 60
           MACAGPIIITAVPHRHIQTPIPFHFPPMARAFLGSTPSSSLLTTSRLSSFTPSLI NQRR
Sbjct: 1   MACAGPIIITAVPHRHIQTPIPFHFPPMARAFLGSTPSSSLLTTSRLSSFTPSLINNQRR 60

Query: 61  RSLRLTTAAVVAMSEFPSEKPIQPHPSLEVIGGGRDRFLPALKDLNLPYKPFPVIGWNRH 120
           RSLRLTTAAVVAMSEFPSEKPIQPHPSLEVIGGG DRFLPALKDLNLPYKPFPVIGWNRH
Sbjct: 61  RSLRLTTAAVVAMSEFPSEKPIQPHPSLEVIGGGCDRFLPALKDLNLPYKPFPVIGWNRH 120

Query: 121 LETIFASFYRSCPDVRFRRECLRAADNGTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQ 180
           LETIFASFYRSCPDVRFRRECLRAADNGTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQ
Sbjct: 121 LETIFASFYRSCPDVRFRRECLRAADNGTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQ 180

Query: 181 DSYVRHMLVRARDRGWRAVVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKAN 240
           DSYVRHMLVRARDRGWRAVVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKAN
Sbjct: 181 DSYVRHMLVRARDRGWRAVVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKAN 240

Query: 241 VYAVGWSLGGNILVNYLGQDSLSCPLSGAVSLCNPFDLVVADEDFRKGFNNVYDKALARA 300
           VYAVGWSLGGNILVNYLGQDSLSCPLSGAVSLCNPFDLVVADEDFRKGFNNVYDKALARA
Sbjct: 241 VYAVGWSLGGNILVNYLGQDSLSCPLSGAVSLCNPFDLVVADEDFRKGFNNVYDKALARA 300

Query: 301 LRRIFSKHADLFNDMPGEFNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFI 360
           LRRIFSKHADLFNDMPGEFNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFI
Sbjct: 301 LRRIFSKHADLFNDMPGEFNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFI 360

Query: 361 KDVKIPLLCIQAANDPIAPDRGVPREDIKANPNCLLIVTPKGGHLGWVAGPSAPLGNPWT 420
           KDVKIPLLCIQAANDPIAPDRGVPREDIK NPNCLLIVTPKGGHLGWVAGPSAPLGNPWT
Sbjct: 361 KDVKIPLLCIQAANDPIAPDRGVPREDIKENPNCLLIVTPKGGHLGWVAGPSAPLGNPWT 420

Query: 421 DPFVIDFLVYLESQESKASASSTDLTAAETLV 453
           DPFVIDFLVYLESQESKASASSTDLTAAETLV
Sbjct: 421 DPFVIDFLVYLESQESKASASSTDLTAAETLV 452

BLAST of CmaCh03G004550.1 vs. ExPASy TrEMBL
Match: A0A5A7TMR0 (Embryogenesis-associated protein EMB8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001510 PE=4 SV=1)

HSP 1 Score: 770.0 bits (1987), Expect = 5.5e-219
Identity = 373/433 (86.14%), Postives = 399/433 (92.15%), Query Frame = 0

Query: 20  PIPFHFPPMARAFLGSTPSSSLLTTSRLSSFTPS-LITNQRRRSLRLTTAAVVAMSEFPS 79
           PIP H PPMARAFLGST SSS L   R+SSFTPS  +TNQR RSLR TT +VVAM+E PS
Sbjct: 26  PIPSHSPPMARAFLGSTSSSSPLIALRISSFTPSFFLTNQRPRSLRPTTGSVVAMAEVPS 85

Query: 80  EKPIQPHPSLEVIGGGRDRFLPALKDLNLPYKPFPVIGWNRHLETIFASFYRSCPDVRFR 139
           EK I+PHPSLEVIGGG D+FLPA KDL+LPYKPFPVIG NRHLETIFASF+R+CP V   
Sbjct: 86  EKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPSVNLH 145

Query: 140 RECLRAADNGTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQDSYVRHMLVRARDRGWRA 199
           RECLRAADNGTVALDWV GDDLRLP+D+PVLILLPGLTGGSQDSYVRHMLVRARDRGWR 
Sbjct: 146 RECLRAADNGTVALDWVTGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRV 205

Query: 200 VVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKANVYAVGWSLGGNILVNYLG 259
           VVFNSRGCGNSPVTTPQFYSASFLGDMREVV HVTKRYPKANVYAVGWSLGGNILVNYLG
Sbjct: 206 VVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTKRYPKANVYAVGWSLGGNILVNYLG 265

Query: 260 QDSLSCPLSGAVSLCNPFDLVVADEDFRKGFNNVYDKALARALRRIFSKHADLFNDMPGE 319
           QDSL+CPLSGAVSLCNPFDL+VADEDFRKGFNNVYDK+LARALR+IF+KHA LF DMP E
Sbjct: 266 QDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVYDKSLARALRKIFNKHAALFTDMPSE 325

Query: 320 FNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIA 379
           FNI LAA ARTVR+YDDALTRVSFGFN+VDEYYSKSSSSKFIKDVKIPLLCIQAANDPIA
Sbjct: 326 FNIRLAAKARTVRDYDDALTRVSFGFNTVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIA 385

Query: 380 PDRGVPREDIKANPNCLLIVTPKGGHLGWVAGPSAPLGNPWTDPFVIDFLVYLESQESKA 439
           P+RG+PREDIK NPNC+LIVTP+GGHLGWVAGP APLG PWTDPFVIDFL+YLE+Q+SKA
Sbjct: 386 PNRGIPREDIKENPNCMLIVTPRGGHLGWVAGPGAPLGFPWTDPFVIDFLMYLENQKSKA 445

Query: 440 SASSTDLTAAETL 452
           SASSTDLT AE +
Sbjct: 446 SASSTDLTTAEVM 458

BLAST of CmaCh03G004550.1 vs. ExPASy TrEMBL
Match: A0A1S3AU57 (LOW QUALITY PROTEIN: embryogenesis-associated protein EMB8 OS=Cucumis melo OX=3656 GN=LOC103482859 PE=4 SV=1)

HSP 1 Score: 770.0 bits (1987), Expect = 5.5e-219
Identity = 373/433 (86.14%), Postives = 399/433 (92.15%), Query Frame = 0

Query: 20  PIPFHFPPMARAFLGSTPSSSLLTTSRLSSFTPS-LITNQRRRSLRLTTAAVVAMSEFPS 79
           PIP H PPMARAFLGST SSS L   R+SSFTPS  +TNQR RSLR TT +VVAM+E PS
Sbjct: 19  PIPSHSPPMARAFLGSTSSSSPLIALRISSFTPSFFLTNQRPRSLRPTTGSVVAMAEVPS 78

Query: 80  EKPIQPHPSLEVIGGGRDRFLPALKDLNLPYKPFPVIGWNRHLETIFASFYRSCPDVRFR 139
           EK I+PHPSLEVIGGG D+FLPA KDL+LPYKPFPVIG NRHLETIFASF+R+CP V   
Sbjct: 79  EKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPSVNLH 138

Query: 140 RECLRAADNGTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQDSYVRHMLVRARDRGWRA 199
           RECLRAADNGTVALDWV GDDLRLP+D+PVLILLPGLTGGSQDSYVRHMLVRARDRGWR 
Sbjct: 139 RECLRAADNGTVALDWVTGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRV 198

Query: 200 VVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKANVYAVGWSLGGNILVNYLG 259
           VVFNSRGCGNSPVTTPQFYSASFLGDMREVV HVTKRYPKANVYAVGWSLGGNILVNYLG
Sbjct: 199 VVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTKRYPKANVYAVGWSLGGNILVNYLG 258

Query: 260 QDSLSCPLSGAVSLCNPFDLVVADEDFRKGFNNVYDKALARALRRIFSKHADLFNDMPGE 319
           QDSL+CPLSGAVSLCNPFDL+VADEDFRKGFNNVYDK+LARALR+IF+KHA LF DMP E
Sbjct: 259 QDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVYDKSLARALRKIFNKHAALFTDMPSE 318

Query: 320 FNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIA 379
           FNI LAA ARTVR+YDDALTRVSFGFN+VDEYYSKSSSSKFIKDVKIPLLCIQAANDPIA
Sbjct: 319 FNIRLAAKARTVRDYDDALTRVSFGFNTVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIA 378

Query: 380 PDRGVPREDIKANPNCLLIVTPKGGHLGWVAGPSAPLGNPWTDPFVIDFLVYLESQESKA 439
           P+RG+PREDIK NPNC+LIVTP+GGHLGWVAGP APLG PWTDPFVIDFL+YLE+Q+SKA
Sbjct: 379 PNRGIPREDIKENPNCMLIVTPRGGHLGWVAGPGAPLGFPWTDPFVIDFLMYLENQKSKA 438

Query: 440 SASSTDLTAAETL 452
           SASSTDLT AE +
Sbjct: 439 SASSTDLTTAEVM 451

BLAST of CmaCh03G004550.1 vs. ExPASy TrEMBL
Match: A0A0A0KNN2 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G148530 PE=4 SV=1)

HSP 1 Score: 760.8 bits (1963), Expect = 3.3e-216
Identity = 369/433 (85.22%), Postives = 396/433 (91.45%), Query Frame = 0

Query: 20  PIPFHFPPMARAFLGSTPSSSLLTTSRLSSFTPS-LITNQRRRSLRLTTAAVVAMSEFPS 79
           PIP H PPMARAFLGSTPSSS L   RLSSFTPS  +TNQR RSLRLTT  VV M+E PS
Sbjct: 47  PIPSHSPPMARAFLGSTPSSSPLIALRLSSFTPSFFLTNQRPRSLRLTTDTVVPMAELPS 106

Query: 80  EKPIQPHPSLEVIGGGRDRFLPALKDLNLPYKPFPVIGWNRHLETIFASFYRSCPDVRFR 139
           EK I+PHPSLEVIGGG D+FLPA KDL+LPYKPFPVIG NRHLETIFASF+R+CP V   
Sbjct: 107 EKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPSVNLH 166

Query: 140 RECLRAADNGTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQDSYVRHMLVRARDRGWRA 199
           RECLRAADNGTVALDWV GDDLRLP+D+PVLILLPGLTGGSQDSYVRHMLVRARDRGWR 
Sbjct: 167 RECLRAADNGTVALDWVAGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRV 226

Query: 200 VVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKANVYAVGWSLGGNILVNYLG 259
           VVFNSRGCGNSPVTTPQFYSASFLGDMREVV HVT+RYPKAN+YAVGWSLGGNILVNYLG
Sbjct: 227 VVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTERYPKANLYAVGWSLGGNILVNYLG 286

Query: 260 QDSLSCPLSGAVSLCNPFDLVVADEDFRKGFNNVYDKALARALRRIFSKHADLFNDMPGE 319
           QDSL+CPLSGAVSLCNPFDL+VADEDFRKGFNNVYDK+LA ALR+IF+KHA LF DM GE
Sbjct: 287 QDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVYDKSLAGALRKIFNKHAALFTDMTGE 346

Query: 320 FNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIA 379
           FNI LAA A TVR+YDDALTRVSFGFN+VDEYYSKSSSSKFIKDVKIPLLCIQAANDPIA
Sbjct: 347 FNIRLAAQATTVRDYDDALTRVSFGFNTVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIA 406

Query: 380 PDRGVPREDIKANPNCLLIVTPKGGHLGWVAGPSAPLGNPWTDPFVIDFLVYLESQESKA 439
           P+RG+PREDI+ NPNC+LIVTPKGGHLGWVAGP APLG PWTDPFVIDFL+YLE+++ KA
Sbjct: 407 PNRGIPREDIEENPNCMLIVTPKGGHLGWVAGPGAPLGFPWTDPFVIDFLMYLENKKFKA 466

Query: 440 SASSTDLTAAETL 452
           SASSTDLT AE +
Sbjct: 467 SASSTDLTMAEVM 479

BLAST of CmaCh03G004550.1 vs. NCBI nr
Match: XP_022977772.1 (embryogenesis-associated protein EMB8-like [Cucurbita maxima])

HSP 1 Score: 926.4 bits (2393), Expect = 9.4e-266
Identity = 452/452 (100.00%), Postives = 452/452 (100.00%), Query Frame = 0

Query: 1   MACAGPIIITAVPHRHIQTPIPFHFPPMARAFLGSTPSSSLLTTSRLSSFTPSLITNQRR 60
           MACAGPIIITAVPHRHIQTPIPFHFPPMARAFLGSTPSSSLLTTSRLSSFTPSLITNQRR
Sbjct: 1   MACAGPIIITAVPHRHIQTPIPFHFPPMARAFLGSTPSSSLLTTSRLSSFTPSLITNQRR 60

Query: 61  RSLRLTTAAVVAMSEFPSEKPIQPHPSLEVIGGGRDRFLPALKDLNLPYKPFPVIGWNRH 120
           RSLRLTTAAVVAMSEFPSEKPIQPHPSLEVIGGGRDRFLPALKDLNLPYKPFPVIGWNRH
Sbjct: 61  RSLRLTTAAVVAMSEFPSEKPIQPHPSLEVIGGGRDRFLPALKDLNLPYKPFPVIGWNRH 120

Query: 121 LETIFASFYRSCPDVRFRRECLRAADNGTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQ 180
           LETIFASFYRSCPDVRFRRECLRAADNGTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQ
Sbjct: 121 LETIFASFYRSCPDVRFRRECLRAADNGTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQ 180

Query: 181 DSYVRHMLVRARDRGWRAVVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKAN 240
           DSYVRHMLVRARDRGWRAVVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKAN
Sbjct: 181 DSYVRHMLVRARDRGWRAVVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKAN 240

Query: 241 VYAVGWSLGGNILVNYLGQDSLSCPLSGAVSLCNPFDLVVADEDFRKGFNNVYDKALARA 300
           VYAVGWSLGGNILVNYLGQDSLSCPLSGAVSLCNPFDLVVADEDFRKGFNNVYDKALARA
Sbjct: 241 VYAVGWSLGGNILVNYLGQDSLSCPLSGAVSLCNPFDLVVADEDFRKGFNNVYDKALARA 300

Query: 301 LRRIFSKHADLFNDMPGEFNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFI 360
           LRRIFSKHADLFNDMPGEFNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFI
Sbjct: 301 LRRIFSKHADLFNDMPGEFNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFI 360

Query: 361 KDVKIPLLCIQAANDPIAPDRGVPREDIKANPNCLLIVTPKGGHLGWVAGPSAPLGNPWT 420
           KDVKIPLLCIQAANDPIAPDRGVPREDIKANPNCLLIVTPKGGHLGWVAGPSAPLGNPWT
Sbjct: 361 KDVKIPLLCIQAANDPIAPDRGVPREDIKANPNCLLIVTPKGGHLGWVAGPSAPLGNPWT 420

Query: 421 DPFVIDFLVYLESQESKASASSTDLTAAETLV 453
           DPFVIDFLVYLESQESKASASSTDLTAAETLV
Sbjct: 421 DPFVIDFLVYLESQESKASASSTDLTAAETLV 452

BLAST of CmaCh03G004550.1 vs. NCBI nr
Match: KAG6603687.1 (Embryogenesis-associated protein EMB8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 923.3 bits (2385), Expect = 8.0e-265
Identity = 451/452 (99.78%), Postives = 451/452 (99.78%), Query Frame = 0

Query: 1   MACAGPIIITAVPHRHIQTPIPFHFPPMARAFLGSTPSSSLLTTSRLSSFTPSLITNQRR 60
           MACAGPIIITAVPHRHIQTPIPFHFPPMARAFLGSTPSSSLLTTSRLSSFTPSLITNQRR
Sbjct: 1   MACAGPIIITAVPHRHIQTPIPFHFPPMARAFLGSTPSSSLLTTSRLSSFTPSLITNQRR 60

Query: 61  RSLRLTTAAVVAMSEFPSEKPIQPHPSLEVIGGGRDRFLPALKDLNLPYKPFPVIGWNRH 120
           RSLRLTTAAVVAMSEFPSEKPIQPHPSLEVIGGG DRFLPALKDLNLPYKPFPVIGWNRH
Sbjct: 61  RSLRLTTAAVVAMSEFPSEKPIQPHPSLEVIGGGCDRFLPALKDLNLPYKPFPVIGWNRH 120

Query: 121 LETIFASFYRSCPDVRFRRECLRAADNGTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQ 180
           LETIFASFYRSCPDVRFRRECLRAADNGTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQ
Sbjct: 121 LETIFASFYRSCPDVRFRRECLRAADNGTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQ 180

Query: 181 DSYVRHMLVRARDRGWRAVVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKAN 240
           DSYVRHMLVRARDRGWRAVVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKAN
Sbjct: 181 DSYVRHMLVRARDRGWRAVVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKAN 240

Query: 241 VYAVGWSLGGNILVNYLGQDSLSCPLSGAVSLCNPFDLVVADEDFRKGFNNVYDKALARA 300
           VYAVGWSLGGNILVNYLGQDSLSCPLSGAVSLCNPFDLVVADEDFRKGFNNVYDKALARA
Sbjct: 241 VYAVGWSLGGNILVNYLGQDSLSCPLSGAVSLCNPFDLVVADEDFRKGFNNVYDKALARA 300

Query: 301 LRRIFSKHADLFNDMPGEFNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFI 360
           LRRIFSKHADLFNDMPGEFNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFI
Sbjct: 301 LRRIFSKHADLFNDMPGEFNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFI 360

Query: 361 KDVKIPLLCIQAANDPIAPDRGVPREDIKANPNCLLIVTPKGGHLGWVAGPSAPLGNPWT 420
           KDVKIPLLCIQAANDPIAPDRGVPREDIKANPNCLLIVTPKGGHLGWVAGPSAPLGNPWT
Sbjct: 361 KDVKIPLLCIQAANDPIAPDRGVPREDIKANPNCLLIVTPKGGHLGWVAGPSAPLGNPWT 420

Query: 421 DPFVIDFLVYLESQESKASASSTDLTAAETLV 453
           DPFVIDFLVYLESQESKASASSTDLTAAETLV
Sbjct: 421 DPFVIDFLVYLESQESKASASSTDLTAAETLV 452

BLAST of CmaCh03G004550.1 vs. NCBI nr
Match: XP_022949852.1 (embryogenesis-associated protein EMB8-like [Cucurbita moschata])

HSP 1 Score: 919.5 bits (2375), Expect = 1.2e-263
Identity = 449/452 (99.34%), Postives = 449/452 (99.34%), Query Frame = 0

Query: 1   MACAGPIIITAVPHRHIQTPIPFHFPPMARAFLGSTPSSSLLTTSRLSSFTPSLITNQRR 60
           MACAGPIIITAVPHRHIQTPIPFHFPPMARAFLGSTPSSSLLTTSRLSSFTPSLI NQRR
Sbjct: 1   MACAGPIIITAVPHRHIQTPIPFHFPPMARAFLGSTPSSSLLTTSRLSSFTPSLINNQRR 60

Query: 61  RSLRLTTAAVVAMSEFPSEKPIQPHPSLEVIGGGRDRFLPALKDLNLPYKPFPVIGWNRH 120
           RSLRLTTAAVVAMSEFPSEKPIQPHPSLEVIGGG DRFLPALKDLNLPYKPFPVIGWNRH
Sbjct: 61  RSLRLTTAAVVAMSEFPSEKPIQPHPSLEVIGGGCDRFLPALKDLNLPYKPFPVIGWNRH 120

Query: 121 LETIFASFYRSCPDVRFRRECLRAADNGTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQ 180
           LETIFASFYRSCPDVRFRRECLRAADNGTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQ
Sbjct: 121 LETIFASFYRSCPDVRFRRECLRAADNGTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQ 180

Query: 181 DSYVRHMLVRARDRGWRAVVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKAN 240
           DSYVRHMLVRARDRGWRAVVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKAN
Sbjct: 181 DSYVRHMLVRARDRGWRAVVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKAN 240

Query: 241 VYAVGWSLGGNILVNYLGQDSLSCPLSGAVSLCNPFDLVVADEDFRKGFNNVYDKALARA 300
           VYAVGWSLGGNILVNYLGQDSLSCPLSGAVSLCNPFDLVVADEDFRKGFNNVYDKALARA
Sbjct: 241 VYAVGWSLGGNILVNYLGQDSLSCPLSGAVSLCNPFDLVVADEDFRKGFNNVYDKALARA 300

Query: 301 LRRIFSKHADLFNDMPGEFNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFI 360
           LRRIFSKHADLFNDMPGEFNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFI
Sbjct: 301 LRRIFSKHADLFNDMPGEFNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFI 360

Query: 361 KDVKIPLLCIQAANDPIAPDRGVPREDIKANPNCLLIVTPKGGHLGWVAGPSAPLGNPWT 420
           KDVKIPLLCIQAANDPIAPDRGVPREDIK NPNCLLIVTPKGGHLGWVAGPSAPLGNPWT
Sbjct: 361 KDVKIPLLCIQAANDPIAPDRGVPREDIKENPNCLLIVTPKGGHLGWVAGPSAPLGNPWT 420

Query: 421 DPFVIDFLVYLESQESKASASSTDLTAAETLV 453
           DPFVIDFLVYLESQESKASASSTDLTAAETLV
Sbjct: 421 DPFVIDFLVYLESQESKASASSTDLTAAETLV 452

BLAST of CmaCh03G004550.1 vs. NCBI nr
Match: XP_023545020.1 (embryogenesis-associated protein EMB8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 916.0 bits (2366), Expect = 1.3e-262
Identity = 448/452 (99.12%), Postives = 449/452 (99.34%), Query Frame = 0

Query: 1   MACAGPIIITAVPHRHIQTPIPFHFPPMARAFLGSTPSSSLLTTSRLSSFTPSLITNQRR 60
           MACAGPIIITAVPHRHIQT IPFHFPPMARAFLGSTPSSSLLTTSRLSSFTPSLITNQRR
Sbjct: 1   MACAGPIIITAVPHRHIQTRIPFHFPPMARAFLGSTPSSSLLTTSRLSSFTPSLITNQRR 60

Query: 61  RSLRLTTAAVVAMSEFPSEKPIQPHPSLEVIGGGRDRFLPALKDLNLPYKPFPVIGWNRH 120
           RSLRLTTAAVVAMSEFPSEKPIQPHPSLEVIGGG DRFLPALKDLNLPYKPFPVIGWNRH
Sbjct: 61  RSLRLTTAAVVAMSEFPSEKPIQPHPSLEVIGGGCDRFLPALKDLNLPYKPFPVIGWNRH 120

Query: 121 LETIFASFYRSCPDVRFRRECLRAADNGTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQ 180
           LETIFASFYRSCPDVRFRRECLRAADNGTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQ
Sbjct: 121 LETIFASFYRSCPDVRFRRECLRAADNGTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQ 180

Query: 181 DSYVRHMLVRARDRGWRAVVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKAN 240
           DSYVRHMLVRARDRGWRAVVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKAN
Sbjct: 181 DSYVRHMLVRARDRGWRAVVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKAN 240

Query: 241 VYAVGWSLGGNILVNYLGQDSLSCPLSGAVSLCNPFDLVVADEDFRKGFNNVYDKALARA 300
           VYAVGWSLGGNILVNYLGQDS SCPLSGAVSLCNPFDLVVADEDFRKGFNNVYDKALARA
Sbjct: 241 VYAVGWSLGGNILVNYLGQDSSSCPLSGAVSLCNPFDLVVADEDFRKGFNNVYDKALARA 300

Query: 301 LRRIFSKHADLFNDMPGEFNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFI 360
           LRRIFSKHADLFNDMPGEFNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFI
Sbjct: 301 LRRIFSKHADLFNDMPGEFNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFI 360

Query: 361 KDVKIPLLCIQAANDPIAPDRGVPREDIKANPNCLLIVTPKGGHLGWVAGPSAPLGNPWT 420
           KDVKIPLLCIQAANDPIAPDRGVPRE+IKANPNCLLIVTPKGGHLGWVAGPSAPLGNPWT
Sbjct: 361 KDVKIPLLCIQAANDPIAPDRGVPREEIKANPNCLLIVTPKGGHLGWVAGPSAPLGNPWT 420

Query: 421 DPFVIDFLVYLESQESKASASSTDLTAAETLV 453
           DPFVIDFLVYLESQESKASASSTDLTAAETLV
Sbjct: 421 DPFVIDFLVYLESQESKASASSTDLTAAETLV 452

BLAST of CmaCh03G004550.1 vs. NCBI nr
Match: KAG7033864.1 (Embryogenesis-associated protein EMB8 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 864.8 bits (2233), Expect = 3.4e-247
Identity = 424/425 (99.76%), Postives = 424/425 (99.76%), Query Frame = 0

Query: 28  MARAFLGSTPSSSLLTTSRLSSFTPSLITNQRRRSLRLTTAAVVAMSEFPSEKPIQPHPS 87
           MARAFLGSTPSSSLLTTSRLSSFTPSLITNQRRRSLRLTTAAVVAMSEFPSEKPIQPHPS
Sbjct: 1   MARAFLGSTPSSSLLTTSRLSSFTPSLITNQRRRSLRLTTAAVVAMSEFPSEKPIQPHPS 60

Query: 88  LEVIGGGRDRFLPALKDLNLPYKPFPVIGWNRHLETIFASFYRSCPDVRFRRECLRAADN 147
           LEVIGGG DRFLPALKDLNLPYKPFPVIGWNRHLETIFASFYRSCPDVRFRRECLRAADN
Sbjct: 61  LEVIGGGCDRFLPALKDLNLPYKPFPVIGWNRHLETIFASFYRSCPDVRFRRECLRAADN 120

Query: 148 GTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQDSYVRHMLVRARDRGWRAVVFNSRGCG 207
           GTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQDSYVRHMLVRARDRGWRAVVFNSRGCG
Sbjct: 121 GTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQDSYVRHMLVRARDRGWRAVVFNSRGCG 180

Query: 208 NSPVTTPQFYSASFLGDMREVVEHVTKRYPKANVYAVGWSLGGNILVNYLGQDSLSCPLS 267
           NSPVTTPQFYSASFLGDMREVVEHVTKRYPKANVYAVGWSLGGNILVNYLGQDSLSCPLS
Sbjct: 181 NSPVTTPQFYSASFLGDMREVVEHVTKRYPKANVYAVGWSLGGNILVNYLGQDSLSCPLS 240

Query: 268 GAVSLCNPFDLVVADEDFRKGFNNVYDKALARALRRIFSKHADLFNDMPGEFNIPLAANA 327
           GAVSLCNPFDLVVADEDFRKGFNNVYDKALARALRRIFSKHADLFNDMPGEFNIPLAANA
Sbjct: 241 GAVSLCNPFDLVVADEDFRKGFNNVYDKALARALRRIFSKHADLFNDMPGEFNIPLAANA 300

Query: 328 RTVREYDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPDRGVPRED 387
           RTVREYDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPDRGVPRED
Sbjct: 301 RTVREYDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPDRGVPRED 360

Query: 388 IKANPNCLLIVTPKGGHLGWVAGPSAPLGNPWTDPFVIDFLVYLESQESKASASSTDLTA 447
           IKANPNCLLIVTPKGGHLGWVAGPSAPLGNPWTDPFVIDFLVYLESQESKASASSTDLTA
Sbjct: 361 IKANPNCLLIVTPKGGHLGWVAGPSAPLGNPWTDPFVIDFLVYLESQESKASASSTDLTA 420

Query: 448 AETLV 453
           AETLV
Sbjct: 421 AETLV 425

BLAST of CmaCh03G004550.1 vs. TAIR 10
Match: AT3G50790.1 (esterase/lipase/thioesterase family protein )

HSP 1 Score: 579.7 bits (1493), Expect = 2.0e-165
Identity = 279/389 (71.72%), Postives = 327/389 (84.06%), Query Frame = 0

Query: 51  TPSLITNQR--RRSLRLTTAAVVAMSEFPSEKPIQPHPSLEVIGGGRDRFLPALKD-LNL 110
           +P LI++ R  RR   +  AA   MS  PS      H SLEVIGGG DRFLP+LKD L  
Sbjct: 10  SPPLISSVRTLRRHFSVFAAANPEMSR-PSH-----HSSLEVIGGGSDRFLPSLKDSLAK 69

Query: 111 PYKPFPVIGWNRHLETIFASFYRSCPDVRFRRECLRAADNGTVALDWVVGDDLRLPIDAP 170
           PY  FP+IG+NRH+ETI+ASF+RS P VR RRECLR  DNG+VALDWV G+D   P D+P
Sbjct: 70  PYNAFPLIGFNRHVETIYASFFRSVPFVRLRRECLRTKDNGSVALDWVAGEDRHFPPDSP 129

Query: 171 VLILLPGLTGGSQDSYVRHMLVRARDRGWRAVVFNSRGCGNSPVTTPQFYSASFLGDMRE 230
           +LILLPGLTGGSQDSYVRHML+RA+ + WR VVFNSRGCG+SPVTTPQFYSASFLGD+ E
Sbjct: 130 ILILLPGLTGGSQDSYVRHMLLRAQSKKWRCVVFNSRGCGDSPVTTPQFYSASFLGDIGE 189

Query: 231 VVEHVTKRYPKANVYAVGWSLGGNILVNYLGQDSLSCPLSGAVSLCNPFDLVVADEDFRK 290
           V++HV  ++PKAN+YA GWSLGGNILVNYLGQ+S +CPL+ AVSLCNPFDLV+ADEDF K
Sbjct: 190 VIDHVVDKFPKANLYAAGWSLGGNILVNYLGQESHNCPLTAAVSLCNPFDLVIADEDFHK 249

Query: 291 GFNNVYDKALARALRRIFSKHADLFNDMPGEFNIPLAANARTVREYDDALTRVSFGFNSV 350
           GFNNVYDKAL+++LRRIFSKH+ LF D+ GEFNIPLAANA TVR++DD LTRVSFGF SV
Sbjct: 250 GFNNVYDKALSKSLRRIFSKHSLLFEDIGGEFNIPLAANAETVRDFDDGLTRVSFGFKSV 309

Query: 351 DEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPDRGVPREDIKANPNCLLIVTPKGGHLGW 410
           DEYYSKSSSSK IK V+IPLLCIQAANDPIAP+RG+PR+DIKANPNC+LIVTP+GGHLGW
Sbjct: 310 DEYYSKSSSSKAIKHVRIPLLCIQAANDPIAPERGIPRDDIKANPNCVLIVTPRGGHLGW 369

Query: 411 VAGPSAPLGNPWTDPFVIDFLVYLESQES 437
           VAG  AP G PWTD  V++FL ++E++E+
Sbjct: 370 VAGEEAPNGAPWTDQVVMEFLQHVENRET 392

BLAST of CmaCh03G004550.1 vs. TAIR 10
Match: AT5G49950.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 223.4 bits (568), Expect = 3.7e-58
Identity = 109/307 (35.50%), Postives = 176/307 (57.33%), Query Frame = 0

Query: 120 HLETIFASFYRSCPDVRFRRECLRAADNGTVALDWVVGDDLRLPID------------AP 179
           HL+T F + +   P   ++R   +A D GT+ALDW++  D+   I              P
Sbjct: 90  HLQTAFLTLFGKSPPFSYKRILYQATDGGTIALDWLMHSDVVEGISQVVNASNPGTDRTP 149

Query: 180 VLILLPGLTGGSQDSYVRHMLVRARDRGWRAVVFNSRGCGNSPVTTPQFYSASFLGDMRE 239
           + I++PGLT  S  +Y++H+  R    GW  VV N RG G   +T+   Y+A +  D+R+
Sbjct: 150 IAIIVPGLTSDSSAAYIKHIAFRLAKEGWNVVVQNHRGLGGISLTSDCVYTAGWTEDLRK 209

Query: 240 VVEHVTKRYPKANVYAVGWSLGGNILVNYLGQDSLSCPLSGAVSLCNPFDLVVADEDF-R 299
           V+ H+  ++P+A ++AVG S+G N+LV YLG+D  + PL GA ++C+P+DL++ D    R
Sbjct: 210 VIAHIHSQFPEAPLFAVGTSIGANVLVKYLGEDGPNTPLIGATAVCSPWDLLICDRFINR 269

Query: 300 KGFNNVYDKALARALRRIFSKHADLFNDMPGEFNIPLAANARTVREYDDALTRVSFGFNS 359
           K    VYD+ L   L+     H  + + +     I     +R+VRE+D+  TR+   F +
Sbjct: 270 KLVQKVYDRMLTIGLQGYAQLHHSIISRIADWEGI---KKSRSVREFDNYATRLVAKFET 329

Query: 360 VDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPDRGVPREDIKANPNCLLIVTPKGGHLG 414
            D YY +SSSS+++++V +PLLCI A +DP+     +P ++ +AN N +L  T  GGHL 
Sbjct: 330 TDTYYRRSSSSQYVENVAVPLLCISALDDPVCTREAIPWDECRANKNIVLATTTHGGHLA 389

BLAST of CmaCh03G004550.1 vs. TAIR 10
Match: AT1G34340.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 212.6 bits (540), Expect = 6.5e-55
Identity = 103/303 (33.99%), Postives = 170/303 (56.11%), Query Frame = 0

Query: 120 HLETIFASFYRSCPDVRFRRECLRAADNGTVALDWVVGDD-----------LRLPIDAPV 179
           H++T F +F+   P   + R+    +D GT+ALDW+   D           +      P+
Sbjct: 96  HIQTCFLNFHGLPPVFTYTRQLFLTSDGGTIALDWLTNSDVLDGSLHNKSEITKEDTTPI 155

Query: 180 LILLPGLTGGSQDSYVRHMLVRARDRGWRAVVFNSRGCGNSPVTTPQFYSASFLGDMREV 239
            +++PGLT  S  +Y++H+       GW  V+ N RG G   VT+  FY+A +  D+R V
Sbjct: 156 AVVIPGLTSDSSSAYLKHLAYDTAKTGWNVVISNHRGLGGVSVTSDCFYNAGWTDDIRVV 215

Query: 240 VEHVTKRYPKANVYAVGWSLGGNILVNYLGQDSLSCPLSGAVSLCNPFDLVVADEDF-RK 299
           ++++  +YP+A ++A+G S+G N+LV YLG++    PL GAV++C+P+DL++ D    R 
Sbjct: 216 LDYLQHKYPRAPLFAIGTSIGANVLVKYLGEEGEKTPLRGAVAICSPWDLLIGDRFICRT 275

Query: 300 GFNNVYDKALARALRRIFSKHADLFNDMPGEFNIPLAANARTVREYDDALTRVSFGFNSV 359
               +YDKAL   L+     H   F  +     I     +R++R++D+  T +   F +V
Sbjct: 276 LKQKLYDKALTIGLQGYAQLHEPQFLRLANWEGI---KKSRSIRDFDNHATCLVGKFETV 335

Query: 360 DEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPDRGVPREDIKANPNCLLIVTPKGGHLGW 411
           D YY KSSS++++ +V +PLLCI A +DP+     +P ++ +AN N +L  T  GGHL +
Sbjct: 336 DTYYRKSSSTQYVGNVAVPLLCISALDDPLCTKEAIPWDECRANKNIVLATTNHGGHLAF 395

BLAST of CmaCh03G004550.1 vs. TAIR 10
Match: AT2G03140.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 163.3 bits (412), Expect = 4.5e-40
Identity = 93/274 (33.94%), Postives = 146/274 (53.28%), Query Frame = 0

Query: 135 VRFRRECLRAADNGTVALDWVVGDDLR--LPIDAPVLILLPGLTGGSQDSYVRHMLVRAR 194
           + ++R C+   D G V+LDW    D+R    +D  V + +PG   GS +  VR  +  A 
Sbjct: 168 LEYQRVCITMEDGGVVSLDWPANLDIREERGLDTTV-VFIPGTPEGSMEEGVRSFVCEAL 227

Query: 195 DRGWRAVVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKANVYAVGWSLGGNI 254
            RG   VV N RGC  SP+TTP+ ++A    D+   +  ++K  P   + AVG   G N+
Sbjct: 228 RRGVFPVVMNPRGCAGSPLTTPRLFTAGDSDDISTALRFLSKTRPWTTLTAVGRGYGANM 287

Query: 255 LVNYLGQDSLSCPLSGAVSLCNPFDLVVADEDFRKG-FNNVYDKALARALRRIFSKHADL 314
           L  YL +     PL+ AV + NPFDL   +E  R   ++   D+ L R L  I   + +L
Sbjct: 288 LTKYLAEAGERTPLTAAVCIDNPFDL---EEITRTSPYSTSLDQQLTRGLVEILLANKEL 347

Query: 315 FNDMPGEFNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQ 374
           F      F++  A  +++VRE+D AL+ V++G  S++++YS  ++ + I +VK+PLL IQ
Sbjct: 348 FQGRAKAFDVGKALCSKSVREFDKALSMVTYGCESIEDFYSSCATREVIGEVKVPLLFIQ 407

Query: 375 AANDPIAPDRGVPREDIKANPNCLLIVTPKGGHL 406
             ND + P   +PR  I  NP   L++     +L
Sbjct: 408 --NDDVVPPYTIPRSSIAENPFTSLLLCSSSPNL 435

BLAST of CmaCh03G004550.1 vs. TAIR 10
Match: AT2G03140.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 163.3 bits (412), Expect = 4.5e-40
Identity = 93/274 (33.94%), Postives = 146/274 (53.28%), Query Frame = 0

Query: 135 VRFRRECLRAADNGTVALDWVVGDDLR--LPIDAPVLILLPGLTGGSQDSYVRHMLVRAR 194
           + ++R C+   D G V+LDW    D+R    +D  V + +PG   GS +  VR  +  A 
Sbjct: 168 LEYQRVCITMEDGGVVSLDWPANLDIREERGLDTTV-VFIPGTPEGSMEEGVRSFVCEAL 227

Query: 195 DRGWRAVVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKANVYAVGWSLGGNI 254
            RG   VV N RGC  SP+TTP+ ++A    D+   +  ++K  P   + AVG   G N+
Sbjct: 228 RRGVFPVVMNPRGCAGSPLTTPRLFTAGDSDDISTALRFLSKTRPWTTLTAVGRGYGANM 287

Query: 255 LVNYLGQDSLSCPLSGAVSLCNPFDLVVADEDFRKG-FNNVYDKALARALRRIFSKHADL 314
           L  YL +     PL+ AV + NPFDL   +E  R   ++   D+ L R L  I   + +L
Sbjct: 288 LTKYLAEAGERTPLTAAVCIDNPFDL---EEITRTSPYSTSLDQQLTRGLVEILLANKEL 347

Query: 315 FNDMPGEFNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQ 374
           F      F++  A  +++VRE+D AL+ V++G  S++++YS  ++ + I +VK+PLL IQ
Sbjct: 348 FQGRAKAFDVGKALCSKSVREFDKALSMVTYGCESIEDFYSSCATREVIGEVKVPLLFIQ 407

Query: 375 AANDPIAPDRGVPREDIKANPNCLLIVTPKGGHL 406
             ND + P   +PR  I  NP   L++     +L
Sbjct: 408 --NDDVVPPYTIPRSSIAENPFTSLLLCSSSPNL 435