CmaCh03G002860.1 (mRNA) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh03G002860.1
Type	mRNA
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Ubiquitin-conjugating enzyme E2 28
Location	Cma_Chr03: 3796320 .. 3798853 (+)
Sequence length	519
RNA-Seq Expression	CmaCh03G002860.1
Synteny	CmaCh03G002860.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGGTAACTCGATAACAAATTCATTGTAAAGGAATCAAAATTGATGGACTAACAATTTCTTAGAACAAAAATTCCTAAACATGAATGTGAAGTAAAAAAAAGAGAGGAATGGATAAGATTAGAATGCGGTGATAAAAGAAATGGAGGATTTGGGATCGCTATAAATATTTAAAGTCTTCTATCATTGCTTAGCGATTGGCAAAGTTCTTCGGTCTCTCAAGTTTGTCAAATTCCACAAAAAATCAGAGGGTAAAGAGACCGACGAACAAGCAGCGGTTTCTGGGGGCCGCCATCGATCTGATTCGGAGCTTATTTCCCTTTCCATAGCTCTTTCCCGCCGATCCTGATCTTCATCCCCTTGTCGATCGCAAACCCTTTTGTTTTCTCAAGGTATTTCTCTCATTTCCATCCGATTTTGTTGGTTTTTTTTTTTGTTTTGTTTTGTTTTTTGTTAATCGCATATCCCTTTGTTTGCTCTGTTCTGAGCTTGATGGATTCTTTGAATCATCTCGTTTTCTAATCGATTTTATGTGTAGATCATGGCTTCGAAGCGTATCTTGAAGGAACTGAAGGATTTACAGAAGGATCCGCCCACATCTTGCAGCGCTGGTATGGTTCTAAGCCCTTGATCTCTTTATTTGATGATCTATGTTGAATGAATTGGTTTCTGTTCATTTGCTGTTTGTTCTATCGTTTTTTCCTCTTAGCAGATGGTATGATTGTGTTGTTGACCTTTAGATCCTGTCTTGATGTGAACATGTTTTTTCTTTCTAGTTTTGAGCTTGGGATGTTTTGTTTTTTGTTCTTTTTTTGTTTATCTTTGCTTTTGAGGATAGGTCCTGTAGCTGAAGACATGTTCCATTGGCAAGCAACCATTATGGGACCATCTGATAGTCCTTATTCAGGAGGTGTTTTTCTAGTCAATATTCATTTCCCTCCAGATTATCCTTTCAAGCCACCAAAGGTGAATGATGTTTGTCTTCTGTTTATGATCTGTGTAATATGATTCTATGTTTCTTGTCTTGTTTTCGAGTAAATAGGATTGCGAGGTTTAATGATTTTGTTTTGTTGTTCAGGTTGCTTTTAGGACCAAAGTGTTTCATCCAAATATCAATAGCAATGGAAGCATTTGTCTCGACATCCTTAAAGAACAGTGGAGCCCCGCGCTTACGATTTCTAAGGTTCGTTTGGCGTTTTCTTGAATTCACCCTCGGCTTTTCGTTTGGTTTCTAAATCTGGATTGAGATTGTGTTGTTTGCATAGACATAGCTGTTTAATGTCGTGTTTGAGATCTATATGCTTCTGTCATTAGTGAAGTTATGTGTATCATCGATGCCTACTCGACTGAAGTCCCTATTCGTATGGCTTAAAATTCACGACCTCGGTTGTATGGCTTAAAATCTTCATTGAATGGATGGCGTATTTCAATTTGTTCTTTTGTTTTCTTGGGTGTCTGTTGTTTGATCACATAAACAATATGAAGACTTGATTGAGCCATGGTTTCTCTATGCAATTGGGAGGTCTTTTTTTGTTTGTTGATATGTTGGGTCTCCGAGTCCTACCCATTGCATTGTTCTTATGGTCTACACTCTATTAATTCGAGCAAGTCGAGAATCGTTGTTAACTTGTGTTTCTACTCCAATAAGCGTGAAGGAAGTAATTGTGTTGTTAACCGCTCAAGCCTATTGTTAGCCGATATTGTGCTCTTTGAGCTTTTCTTTCTGCGCTTCCCCTTAAGGTTTTAAAACGCGTCTATTAGGGAGAGACTTTCACATCTTTATAAGAAAGGTTTCGTTCCCCTCTCCAACTGATGTGAGATCCCACAATCCACCCCCTCAGGGGGCCAGTGTCCTCGTTGGCACACCGTCTAGTGTTTGGTTTTGATACCATTTGTAACAGCCCAAGCCTAGCGCTAAGAGATATTGTCTTCTGGGCTTCCTCTCAAGGTTTTAAAATGAGTCTACTTGGGAGAGGCTTTCATACCCTTATAAGAAATGTTTCGTTCCTCTCTTCAACCAATGTGAGATCTCACAATCCATCCTCTTGGGAGGTCCAGCGTTCTCGTTGGCACACCGCCCGGTGTCTAACTCTAATACCATTTGTAACAGCTCAGCTTACCTCTAGTAGATATTGTCCTCTCTAAGCTTCTCCTCAAGGTTTTAAACCGCGGCTACTAGGGAGAGATTTCCACACCCTTATAAAAAATGCTATCTAAGCCCTCATTCTACCCACACCCCACAATTCTAATAAAGAACAAGTTAAATAGTTGAGCTTTATACCCTCACTATAACCTTTTGATCATTCAACAAAAGCCCATTTCAGCAACAAACTCGCTTTTCATATCGACTTCGAACCAAATTCTAAACATAATGTTGTTCATGAAACAGGTCCTGCTCTCCATATGCTCATTGCTGACCGATCCAAACCCAGACGACCCTCTTGTACCGGAGATTGCACACATGTACAAGACCGACCGAGCCAAGTATGAAGCAAGCGCACGGAGCTGGACTCAGAAGTACGCGATGGGTTGA

mRNA sequence

ATGAAGCTCTTTCCCGCCGATCCTGATCTTCATCCCCTTGTCGATCGCAAACCCTTTTGTTTTCTCAAGATCATGGCTTCGAAGCGTATCTTGAAGGAACTGAAGGATTTACAGAAGGATCCGCCCACATCTTGCAGCGCTGGTCCTGTAGCTGAAGACATGTTCCATTGGCAAGCAACCATTATGGGACCATCTGATAGTCCTTATTCAGGAGGTGTTTTTCTAGTCAATATTCATTTCCCTCCAGATTATCCTTTCAAGCCACCAAAGGTTGCTTTTAGGACCAAAGTGTTTCATCCAAATATCAATAGCAATGGAAGCATTTGTCTCGACATCCTTAAAGAACAGTGGAGCCCCGCGCTTACGATTTCTAAGGTCCTGCTCTCCATATGCTCATTGCTGACCGATCCAAACCCAGACGACCCTCTTGTACCGGAGATTGCACACATGTACAAGACCGACCGAGCCAAGTATGAAGCAAGCGCACGGAGCTGGACTCAGAAGTACGCGATGGGTTGA

Coding sequence (CDS)

ATGAAGCTCTTTCCCGCCGATCCTGATCTTCATCCCCTTGTCGATCGCAAACCCTTTTGTTTTCTCAAGATCATGGCTTCGAAGCGTATCTTGAAGGAACTGAAGGATTTACAGAAGGATCCGCCCACATCTTGCAGCGCTGGTCCTGTAGCTGAAGACATGTTCCATTGGCAAGCAACCATTATGGGACCATCTGATAGTCCTTATTCAGGAGGTGTTTTTCTAGTCAATATTCATTTCCCTCCAGATTATCCTTTCAAGCCACCAAAGGTTGCTTTTAGGACCAAAGTGTTTCATCCAAATATCAATAGCAATGGAAGCATTTGTCTCGACATCCTTAAAGAACAGTGGAGCCCCGCGCTTACGATTTCTAAGGTCCTGCTCTCCATATGCTCATTGCTGACCGATCCAAACCCAGACGACCCTCTTGTACCGGAGATTGCACACATGTACAAGACCGACCGAGCCAAGTATGAAGCAAGCGCACGGAGCTGGACTCAGAAGTACGCGATGGGTTGA

Protein sequence

MKLFPADPDLHPLVDRKPFCFLKIMASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYEASARSWTQKYAMG

Homology

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BLAST of CmaCh03G002860.1 vs. ExPASy Swiss-Prot
Match: Q94F47 (Ubiquitin-conjugating enzyme E2 28 OS=Arabidopsis thaliana OX=3702 GN=UBC28 PE=1 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 7.9e-83
Identity = 144/148 (97.30%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 25  MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNIHFPPDY 84
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLV IHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDY 60

Query: 85  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 144
           PFKPPKVAFRTKVFHPN+NSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNVNSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 145 PEIAHMYKTDRAKYEASARSWTQKYAMG 173
           PEIAHMYKTDRAKYE++ARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYESTARSWTQKYAMG 148

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BLAST of CmaCh03G002860.1 vs. ExPASy Swiss-Prot
Match: P35135 (Ubiquitin-conjugating enzyme E2-17 kDa OS=Solanum lycopersicum OX=4081 PE=2 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 3.0e-82
Identity = 143/148 (96.62%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 25  MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNIHFPPDY 84
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP+DSPY+GGVFLV+IHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPTDSPYAGGVFLVSIHFPPDY 60

Query: 85  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 144
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 145 PEIAHMYKTDRAKYEASARSWTQKYAMG 173
           PEIAHMYKTDRAKYE +ARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYETTARSWTQKYAMG 148

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BLAST of CmaCh03G002860.1 vs. ExPASy Swiss-Prot
Match: P35131 (Ubiquitin-conjugating enzyme E2 8 OS=Arabidopsis thaliana OX=3702 GN=UBC8 PE=1 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 3.9e-82
Identity = 143/148 (96.62%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 25  MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNIHFPPDY 84
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP++SPYSGGVFLV IHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPAESPYSGGVFLVTIHFPPDY 60

Query: 85  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 144
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 145 PEIAHMYKTDRAKYEASARSWTQKYAMG 173
           PEIAHMYKTDRAKYEA+AR+WTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYEATARNWTQKYAMG 148

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BLAST of CmaCh03G002860.1 vs. ExPASy Swiss-Prot
Match: P35132 (SUMO-conjugating enzyme UBC9 OS=Arabidopsis thaliana OX=3702 GN=UBC9 PE=1 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 1.5e-81
Identity = 142/148 (95.95%), Postives = 146/148 (98.65%), Query Frame = 0


Query: 25  MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNIHFPPDY 84
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLV IHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDY 60

Query: 85  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 144
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 145 PEIAHMYKTDRAKYEASARSWTQKYAMG 173
           PEIAHMYKTD+ KYE++AR+WTQKYAMG
Sbjct: 121 PEIAHMYKTDKNKYESTARTWTQKYAMG 148

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BLAST of CmaCh03G002860.1 vs. ExPASy Swiss-Prot
Match: Q8S919 (Ubiquitin-conjugating enzyme E2 5B OS=Oryza sativa subsp. japonica OX=39947 GN=UBC5B PE=2 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 2.6e-81
Identity = 141/148 (95.27%), Postives = 146/148 (98.65%), Query Frame = 0


Query: 25  MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNIHFPPDY 84
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQAT+MGPSDSPY+GGVFLV IHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATLMGPSDSPYAGGVFLVTIHFPPDY 60

Query: 85  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 144
           PFKPPKVA +TKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVALKTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 145 PEIAHMYKTDRAKYEASARSWTQKYAMG 173
           PEIAHMYKTDRAKYE++ARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYESTARSWTQKYAMG 148

[Close]

BLAST of CmaCh03G002860.1 vs. ExPASy TrEMBL
Match: A0A5D3CD22 (Ubiquitin-conjugating enzyme E2-17 kDa OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold127G00350 PE=3 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 6.3e-83
Identity = 150/151 (99.34%), Postives = 151/151 (100.00%), Query Frame = 0


Query: 22  LKIMASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNIHFP 81
           L+IMASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNIHFP
Sbjct: 38  LRIMASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNIHFP 97

Query: 82  PDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDD 141
           PDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDD
Sbjct: 98  PDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDD 157

Query: 142 PLVPEIAHMYKTDRAKYEASARSWTQKYAMG 173
           PLVPEIAHMYKTDRAKYEASARSWTQKYAMG
Sbjct: 158 PLVPEIAHMYKTDRAKYEASARSWTQKYAMG 188

[Close]

BLAST of CmaCh03G002860.1 vs. ExPASy TrEMBL
Match: A0A6J1ISN6 (ubiquitin-conjugating enzyme E2 28-like OS=Cucurbita maxima OX=3661 GN=LOC111478335 PE=3 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 9.1e-82
Identity = 148/148 (100.00%), Postives = 148/148 (100.00%), Query Frame = 0


Query: 25  MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNIHFPPDY 84
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNIHFPPDY 60

Query: 85  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 144
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 145 PEIAHMYKTDRAKYEASARSWTQKYAMG 173
           PEIAHMYKTDRAKYEASARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYEASARSWTQKYAMG 148

[Close]

BLAST of CmaCh03G002860.1 vs. ExPASy TrEMBL
Match: A0A6J1EGH9 (ubiquitin-conjugating enzyme E2 28-like OS=Cucurbita moschata OX=3662 GN=LOC111432341 PE=3 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 9.1e-82
Identity = 148/148 (100.00%), Postives = 148/148 (100.00%), Query Frame = 0


Query: 25  MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNIHFPPDY 84
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNIHFPPDY 60

Query: 85  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 144
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 145 PEIAHMYKTDRAKYEASARSWTQKYAMG 173
           PEIAHMYKTDRAKYEASARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYEASARSWTQKYAMG 148

[Close]

BLAST of CmaCh03G002860.1 vs. ExPASy TrEMBL
Match: A0A0A0L2X5 (UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G334720 PE=3 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 9.1e-82
Identity = 148/148 (100.00%), Postives = 148/148 (100.00%), Query Frame = 0


Query: 25  MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNIHFPPDY 84
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNIHFPPDY 60

Query: 85  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 144
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 145 PEIAHMYKTDRAKYEASARSWTQKYAMG 173
           PEIAHMYKTDRAKYEASARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYEASARSWTQKYAMG 148

[Close]

BLAST of CmaCh03G002860.1 vs. ExPASy TrEMBL
Match: A0A6J1CR94 (ubiquitin-conjugating enzyme E2 28-like OS=Momordica charantia OX=3673 GN=LOC111013862 PE=3 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 9.1e-82
Identity = 148/148 (100.00%), Postives = 148/148 (100.00%), Query Frame = 0


Query: 25  MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNIHFPPDY 84
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNIHFPPDY 60

Query: 85  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 144
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 145 PEIAHMYKTDRAKYEASARSWTQKYAMG 173
           PEIAHMYKTDRAKYEASARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYEASARSWTQKYAMG 148

[Close]

BLAST of CmaCh03G002860.1 vs. NCBI nr
Match: KAG6603527.1 (Ubiquitin-conjugating enzyme E2 28, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 354.8 bits (909), Expect = 4.3e-94
Identity = 167/168 (99.40%), Postives = 167/168 (99.40%), Query Frame = 0


Query: 1   MKLFPADPDLHPLVDRKPFCFLKIMASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQAT 60
           M LFPADPDLHPLVDRKPFCFLKIMASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQAT
Sbjct: 1   MNLFPADPDLHPLVDRKPFCFLKIMASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQAT 60

Query: 61  IMGPSDSPYSGGVFLVNIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPA 120
           IMGPSDSPYSGGVFLVNIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPA
Sbjct: 61  IMGPSDSPYSGGVFLVNIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPA 120

Query: 121 LTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYEASARSWTQK 169
           LTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYEASARSWTQK
Sbjct: 121 LTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYEASARSWTQK 168

[Close]

BLAST of CmaCh03G002860.1 vs. NCBI nr
Match: KAG6595545.1 (Ubiquitin-conjugating enzyme E2 28, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 344.4 bits (882), Expect = 5.8e-91
Identity = 163/168 (97.02%), Postives = 164/168 (97.62%), Query Frame = 0


Query: 5   PADPDLHPLVDRKPFCFLKIMASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP 64
           P   D +PLVDRKPFCFLKIMASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP
Sbjct: 134 PYFSDFYPLVDRKPFCFLKIMASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP 193

Query: 65  SDSPYSGGVFLVNIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTIS 124
           SDSPYSGGVFLVNIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTIS
Sbjct: 194 SDSPYSGGVFLVNIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTIS 253

Query: 125 KVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYEASARSWTQKYAMG 173
           KVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYEASARSWTQKYAMG
Sbjct: 254 KVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYEASARSWTQKYAMG 301

[Close]

BLAST of CmaCh03G002860.1 vs. NCBI nr
Match: TYK09753.1 (ubiquitin-conjugating enzyme E2-17 kDa [Cucumis melo var. makuwa])

HSP 1 Score: 316.6 bits (810), Expect = 1.3e-82
Identity = 150/151 (99.34%), Postives = 151/151 (100.00%), Query Frame = 0


Query: 22  LKIMASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNIHFP 81
           L+IMASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNIHFP
Sbjct: 38  LRIMASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNIHFP 97

Query: 82  PDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDD 141
           PDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDD
Sbjct: 98  PDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDD 157

Query: 142 PLVPEIAHMYKTDRAKYEASARSWTQKYAMG 173
           PLVPEIAHMYKTDRAKYEASARSWTQKYAMG
Sbjct: 158 PLVPEIAHMYKTDRAKYEASARSWTQKYAMG 188

[Close]

BLAST of CmaCh03G002860.1 vs. NCBI nr
Match: XP_004142676.1 (ubiquitin-conjugating enzyme E2 28 [Cucumis sativus] >XP_008463276.1 PREDICTED: ubiquitin-conjugating enzyme E2 28 [Cucumis melo] >XP_022144084.1 ubiquitin-conjugating enzyme E2 28-like [Momordica charantia] >XP_022924950.1 ubiquitin-conjugating enzyme E2 28-like [Cucurbita moschata] >XP_022950142.1 ubiquitin-conjugating enzyme E2 28-like [Cucurbita moschata] >XP_022966476.1 ubiquitin-conjugating enzyme E2 28-like [Cucurbita maxima] >XP_022978308.1 ubiquitin-conjugating enzyme E2 28-like [Cucurbita maxima] >XP_023517728.1 ubiquitin-conjugating enzyme E2 28-like [Cucurbita pepo subsp. pepo] >XP_023517729.1 ubiquitin-conjugating enzyme E2 28-like [Cucurbita pepo subsp. pepo] >XP_023543247.1 ubiquitin-conjugating enzyme E2 28-like [Cucurbita pepo subsp. pepo] >XP_038882512.1 ubiquitin-conjugating enzyme E2 28-like [Benincasa hispida] >KAG6595637.1 Ubiquitin-conjugating enzyme E2 28, partial [Cucurbita argyrosperma subsp. sororia] >KGN54461.1 hypothetical protein Csa_012070 [Cucumis sativus])

HSP 1 Score: 312.8 bits (800), Expect = 1.9e-81
Identity = 148/148 (100.00%), Postives = 148/148 (100.00%), Query Frame = 0


Query: 25  MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNIHFPPDY 84
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNIHFPPDY 60

Query: 85  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 144
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 145 PEIAHMYKTDRAKYEASARSWTQKYAMG 173
           PEIAHMYKTDRAKYEASARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYEASARSWTQKYAMG 148

[Close]

BLAST of CmaCh03G002860.1 vs. NCBI nr
Match: XP_022947819.1 (ubiquitin-conjugating enzyme E2-17 kDa-like [Cucurbita moschata])

HSP 1 Score: 312.8 bits (800), Expect = 1.9e-81
Identity = 147/154 (95.45%), Postives = 150/154 (97.40%), Query Frame = 0


Query: 19  FCFLKIMASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNI 78
           F F K MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP+DSPYSGGVFLV+I
Sbjct: 34  FVFWKFMASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPTDSPYSGGVFLVSI 93

Query: 79  HFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPN 138
           HFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPN
Sbjct: 94  HFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPN 153

Query: 139 PDDPLVPEIAHMYKTDRAKYEASARSWTQKYAMG 173
           PDDPLVPEIAHMYKTDRAKYE +ARSWTQKYAMG
Sbjct: 154 PDDPLVPEIAHMYKTDRAKYEGTARSWTQKYAMG 187

[Close]

BLAST of CmaCh03G002860.1 vs. TAIR 10
Match: AT1G64230.1 (ubiquitin-conjugating enzyme 28 )

HSP 1 Score: 307.8 bits (787), Expect = 5.6e-84
Identity = 144/148 (97.30%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 25  MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNIHFPPDY 84
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLV IHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDY 60

Query: 85  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 144
           PFKPPKVAFRTKVFHPN+NSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNVNSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 145 PEIAHMYKTDRAKYEASARSWTQKYAMG 173
           PEIAHMYKTDRAKYE++ARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYESTARSWTQKYAMG 148

[Close]

BLAST of CmaCh03G002860.1 vs. TAIR 10
Match: AT1G64230.2 (ubiquitin-conjugating enzyme 28 )

HSP 1 Score: 307.8 bits (787), Expect = 5.6e-84
Identity = 144/148 (97.30%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 25  MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNIHFPPDY 84
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLV IHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDY 60

Query: 85  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 144
           PFKPPKVAFRTKVFHPN+NSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNVNSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 145 PEIAHMYKTDRAKYEASARSWTQKYAMG 173
           PEIAHMYKTDRAKYE++ARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYESTARSWTQKYAMG 148

[Close]

BLAST of CmaCh03G002860.1 vs. TAIR 10
Match: AT5G41700.2 (ubiquitin conjugating enzyme 8 )

HSP 1 Score: 305.4 bits (781), Expect = 2.8e-83
Identity = 143/148 (96.62%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 25  MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNIHFPPDY 84
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP++SPYSGGVFLV IHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPAESPYSGGVFLVTIHFPPDY 60

Query: 85  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 144
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 145 PEIAHMYKTDRAKYEASARSWTQKYAMG 173
           PEIAHMYKTDRAKYEA+AR+WTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYEATARNWTQKYAMG 148

[Close]

BLAST of CmaCh03G002860.1 vs. TAIR 10
Match: AT5G41700.1 (ubiquitin conjugating enzyme 8 )

HSP 1 Score: 305.4 bits (781), Expect = 2.8e-83
Identity = 143/148 (96.62%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 25  MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNIHFPPDY 84
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP++SPYSGGVFLV IHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPAESPYSGGVFLVTIHFPPDY 60

Query: 85  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 144
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 145 PEIAHMYKTDRAKYEASARSWTQKYAMG 173
           PEIAHMYKTDRAKYEA+AR+WTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYEATARNWTQKYAMG 148

[Close]

BLAST of CmaCh03G002860.1 vs. TAIR 10
Match: AT5G41700.5 (ubiquitin conjugating enzyme 8 )

HSP 1 Score: 305.4 bits (781), Expect = 2.8e-83
Identity = 143/148 (96.62%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 25  MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNIHFPPDY 84
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP++SPYSGGVFLV IHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPAESPYSGGVFLVTIHFPPDY 60

Query: 85  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 144
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 145 PEIAHMYKTDRAKYEASARSWTQKYAMG 173
           PEIAHMYKTDRAKYEA+AR+WTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYEATARNWTQKYAMG 148

The following BLAST results are available for this feature:

BLAST of CmaCh03G002860.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94F47	7.9e-83	97.30	Ubiquitin-conjugating enzyme E2 28 OS=Arabidopsis thaliana OX=3702 GN=UBC28 PE=1...	[more]
P35135	3.0e-82	96.62	Ubiquitin-conjugating enzyme E2-17 kDa OS=Solanum lycopersicum OX=4081 PE=2 SV=1	[more]
P35131	3.9e-82	96.62	Ubiquitin-conjugating enzyme E2 8 OS=Arabidopsis thaliana OX=3702 GN=UBC8 PE=1 S...	[more]
P35132	1.5e-81	95.95	SUMO-conjugating enzyme UBC9 OS=Arabidopsis thaliana OX=3702 GN=UBC9 PE=1 SV=1	[more]
Q8S919	2.6e-81	95.27	Ubiquitin-conjugating enzyme E2 5B OS=Oryza sativa subsp. japonica OX=39947 GN=U...	[more]

BLAST of CmaCh03G002860.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CD22	6.3e-83	99.34	Ubiquitin-conjugating enzyme E2-17 kDa OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A6J1ISN6	9.1e-82	100.00	ubiquitin-conjugating enzyme E2 28-like OS=Cucurbita maxima OX=3661 GN=LOC111478...	[more]
A0A6J1EGH9	9.1e-82	100.00	ubiquitin-conjugating enzyme E2 28-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A0A0L2X5	9.1e-82	100.00	UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G334720 PE...	[more]
A0A6J1CR94	9.1e-82	100.00	ubiquitin-conjugating enzyme E2 28-like OS=Momordica charantia OX=3673 GN=LOC111...	[more]

BLAST of CmaCh03G002860.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6603527.1	4.3e-94	99.40	Ubiquitin-conjugating enzyme E2 28, partial [Cucurbita argyrosperma subsp. soror...	[more]
KAG6595545.1	5.8e-91	97.02	Ubiquitin-conjugating enzyme E2 28, partial [Cucurbita argyrosperma subsp. soror...	[more]
TYK09753.1	1.3e-82	99.34	ubiquitin-conjugating enzyme E2-17 kDa [Cucumis melo var. makuwa]	[more]
XP_004142676.1	1.9e-81	100.00	ubiquitin-conjugating enzyme E2 28 [Cucumis sativus] >XP_008463276.1 PREDICTED: ...	[more]
XP_022947819.1	1.9e-81	95.45	ubiquitin-conjugating enzyme E2-17 kDa-like [Cucurbita moschata]	[more]

BLAST of CmaCh03G002860.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G64230.1	5.6e-84	97.30	ubiquitin-conjugating enzyme 28	[more]
AT1G64230.2	5.6e-84	97.30	ubiquitin-conjugating enzyme 28	[more]
AT5G41700.2	2.8e-83	96.62	ubiquitin conjugating enzyme 8	[more]
AT5G41700.1	2.8e-83	96.62	ubiquitin conjugating enzyme 8	[more]
AT5G41700.5	2.8e-83	96.62	ubiquitin conjugating enzyme 8	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SMART	SM00212	ubc_7	coord: 28..171 e-value: 1.9E-78 score: 276.6
None	No IPR available	PANTHER	PTHR24068:SF346	BNAA01G32320D PROTEIN	coord: 27..169
None	No IPR available	PANTHER	PTHR24068	UBIQUITIN-CONJUGATING ENZYME E2	coord: 27..169
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	GENE3D	3.10.110.10	Ubiquitin Conjugating Enzyme	coord: 21..172 e-value: 8.9E-75 score: 251.6
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	SUPERFAMILY	54495	UBC-like	coord: 23..171
IPR000608	Ubiquitin-conjugating enzyme E2	PFAM	PF00179	UQ_con	coord: 29..165 e-value: 2.7E-53 score: 179.4
IPR000608	Ubiquitin-conjugating enzyme E2	PROSITE	PS50127	UBC_2	coord: 25..171 score: 60.46505
IPR000608	Ubiquitin-conjugating enzyme E2	CDD	cd00195	UBCc	coord: 27..166 e-value: 2.46446E-73 score: 215.144
IPR023313	Ubiquitin-conjugating enzyme, active site	PROSITE	PS00183	UBC_1	coord: 98..113

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmaCh03G002860	CmaCh03G002860	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh03G002860.1:exon:1073	CmaCh03G002860.1:exon:1073	exon
CmaCh03G002860.1:exon:1074	CmaCh03G002860.1:exon:1074	exon
CmaCh03G002860.1:exon:1075	CmaCh03G002860.1:exon:1075	exon
CmaCh03G002860.1:exon:1076	CmaCh03G002860.1:exon:1076	exon
CmaCh03G002860.1:exon:1077	CmaCh03G002860.1:exon:1077	exon
CmaCh03G002860.1:exon:1078	CmaCh03G002860.1:exon:1078	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh03G002860.1:cds	CmaCh03G002860.1:cds	CDS
CmaCh03G002860.1:cds	CmaCh03G002860.1:cds_2	CDS
CmaCh03G002860.1:cds	CmaCh03G002860.1:cds_3	CDS
CmaCh03G002860.1:cds	CmaCh03G002860.1:cds_4	CDS
CmaCh03G002860.1:cds	CmaCh03G002860.1:cds_5	CDS
CmaCh03G002860.1:cds	CmaCh03G002860.1:cds_6	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmaCh03G002860.1	CmaCh03G002860.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000209	protein polyubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0061631	ubiquitin conjugating enzyme activity