CmaCh03G000780.1 (mRNA) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh03G000780.1
TypemRNA
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionpolygalacturonase inhibitor-like
LocationCma_Chr03: 782690 .. 783676 (-)
Sequence length987
RNA-Seq ExpressionCmaCh03G000780.1
SyntenyCmaCh03G000780.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTTCTTCTCCATCCACCTTCCTCTTAATCTTCTTCCTATTCTTCACCATTTCATTTGCAGAACTCTGCCATCCAAATGACAAGAAAGCCCTTCTCAACATCAAAAAAGCCTTCAACAATCCCTACATTCTCACCTCATGGAAGCCTGAAGAAGATTGTTGCACCTGGTACTGCGTCGAATGTGATCGGAATTCCCACCGGATCGTCTCCCTCACCATCTTCGCTGACACGAGACTCTCGGGACAAATCCCGCCGTTCGTTGGCGACCTCCCGTACCTCGAAAACCTCATGTTCCACAAGCTTCCAAATCTCACTGGCCCCATCCAACCCGCCATTGCCAAACTCCATAACCTAAAATACCTTGACCTAAGTTGGAACACTCTCACTGGTTCAATCCCAAATTTCCTCAGCTCCCTCACCAATCTCGAACTTCTCGACCTCTCTTTCAACCGACTCACCGGCTCCATCCCGAGCTCCCTCGCCAACCTCCAACGCCTTGGCACTCTCCATTTAGACCGAAACAAATTAACCGGACCGATCCCCGATTCATTCGGAAACTTCAAGGGAAAAATCCCATATCTTTATCTTTCACACAACCAACTCTCTGGGAAAATTCCAACTTCCTTCGAGAGAGTGGATTTCAATGTGATTGATTTGTCGAGGAACAGGCTCGAGGGCGATGGATCGGTGATATTCGGAGGGAAGAAGACGACGGAGATCGTCGACTTGTCGAGGAACTTGTTGGAGTTCGACATGTCGAAGGTCGTGCTTCCTCGGAGCTTGACTTGGTTGGATCTTAACCATAATAAGATCTTCGGGAAAATTCCTGTGATGGCTGTTAACTTGCAGTTGCAGCTGCTGAATGTGAGTTACAACAAGCTTTGTGGTCGGATTCCGGTCGGAGGGAAGTTGCAAAGCTTCGATGTCTACTCCTATTCTAATAACAAGTGCTTGTGTGGAAAGCCACTTGGTAAATGCAAATGA

mRNA sequence

ATGTCTTCTTCTCCATCCACCTTCCTCTTAATCTTCTTCCTATTCTTCACCATTTCATTTGCAGAACTCTGCCATCCAAATGACAAGAAAGCCCTTCTCAACATCAAAAAAGCCTTCAACAATCCCTACATTCTCACCTCATGGAAGCCTGAAGAAGATTGTTGCACCTGGTACTGCGTCGAATGTGATCGGAATTCCCACCGGATCGTCTCCCTCACCATCTTCGCTGACACGAGACTCTCGGGACAAATCCCGCCGTTCGTTGGCGACCTCCCGTACCTCGAAAACCTCATGTTCCACAAGCTTCCAAATCTCACTGGCCCCATCCAACCCGCCATTGCCAAACTCCATAACCTAAAATACCTTGACCTAAGTTGGAACACTCTCACTGGTTCAATCCCAAATTTCCTCAGCTCCCTCACCAATCTCGAACTTCTCGACCTCTCTTTCAACCGACTCACCGGCTCCATCCCGAGCTCCCTCGCCAACCTCCAACGCCTTGGCACTCTCCATTTAGACCGAAACAAATTAACCGGACCGATCCCCGATTCATTCGGAAACTTCAAGGGAAAAATCCCATATCTTTATCTTTCACACAACCAACTCTCTGGGAAAATTCCAACTTCCTTCGAGAGAGTGGATTTCAATGTGATTGATTTGTCGAGGAACAGGCTCGAGGGCGATGGATCGGTGATATTCGGAGGGAAGAAGACGACGGAGATCGTCGACTTGTCGAGGAACTTGTTGGAGTTCGACATGTCGAAGGTCGTGCTTCCTCGGAGCTTGACTTGGTTGGATCTTAACCATAATAAGATCTTCGGGAAAATTCCTGTGATGGCTGTTAACTTGCAGTTGCAGCTGCTGAATGTGAGTTACAACAAGCTTTGTGGTCGGATTCCGGTCGGAGGGAAGTTGCAAAGCTTCGATGTCTACTCCTATTCTAATAACAAGTGCTTGTGTGGAAAGCCACTTGGTAAATGCAAATGA

Coding sequence (CDS)

ATGTCTTCTTCTCCATCCACCTTCCTCTTAATCTTCTTCCTATTCTTCACCATTTCATTTGCAGAACTCTGCCATCCAAATGACAAGAAAGCCCTTCTCAACATCAAAAAAGCCTTCAACAATCCCTACATTCTCACCTCATGGAAGCCTGAAGAAGATTGTTGCACCTGGTACTGCGTCGAATGTGATCGGAATTCCCACCGGATCGTCTCCCTCACCATCTTCGCTGACACGAGACTCTCGGGACAAATCCCGCCGTTCGTTGGCGACCTCCCGTACCTCGAAAACCTCATGTTCCACAAGCTTCCAAATCTCACTGGCCCCATCCAACCCGCCATTGCCAAACTCCATAACCTAAAATACCTTGACCTAAGTTGGAACACTCTCACTGGTTCAATCCCAAATTTCCTCAGCTCCCTCACCAATCTCGAACTTCTCGACCTCTCTTTCAACCGACTCACCGGCTCCATCCCGAGCTCCCTCGCCAACCTCCAACGCCTTGGCACTCTCCATTTAGACCGAAACAAATTAACCGGACCGATCCCCGATTCATTCGGAAACTTCAAGGGAAAAATCCCATATCTTTATCTTTCACACAACCAACTCTCTGGGAAAATTCCAACTTCCTTCGAGAGAGTGGATTTCAATGTGATTGATTTGTCGAGGAACAGGCTCGAGGGCGATGGATCGGTGATATTCGGAGGGAAGAAGACGACGGAGATCGTCGACTTGTCGAGGAACTTGTTGGAGTTCGACATGTCGAAGGTCGTGCTTCCTCGGAGCTTGACTTGGTTGGATCTTAACCATAATAAGATCTTCGGGAAAATTCCTGTGATGGCTGTTAACTTGCAGTTGCAGCTGCTGAATGTGAGTTACAACAAGCTTTGTGGTCGGATTCCGGTCGGAGGGAAGTTGCAAAGCTTCGATGTCTACTCCTATTCTAATAACAAGTGCTTGTGTGGAAAGCCACTTGGTAAATGCAAATGA

Protein sequence

MSSSPSTFLLIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCVECDRNSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLKYLDLSWNTLTGSIPNFLSSLTNLELLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGPIPDSFGNFKGKIPYLYLSHNQLSGKIPTSFERVDFNVIDLSRNRLEGDGSVIFGGKKTTEIVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGKIPVMAVNLQLQLLNVSYNKLCGRIPVGGKLQSFDVYSYSNNKCLCGKPLGKCK
Homology
BLAST of CmaCh03G000780.1 vs. ExPASy Swiss-Prot
Match: Q05091 (Polygalacturonase inhibitor OS=Pyrus communis OX=23211 GN=PGIP PE=1 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 1.9e-125
Identity = 221/326 (67.79%), Postives = 254/326 (77.91%), Query Frame = 0

Query: 6   STFLLIFFLFFTI---SFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCVEC 65
           STFL +  LF ++   + ++LC+P+DKK LL IKKAF +PY+L SWK + DCC WYCV C
Sbjct: 6   STFLSLTLLFSSVLNPALSDLCNPDDKKVLLQIKKAFGDPYVLASWKSDTDCCDWYCVTC 65

Query: 66  DRNSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLKYL 125
           D  ++RI SLTIFA  ++SGQIP  VGDLPYLE L FHK PNLTGPIQPAIAKL  LK L
Sbjct: 66  DSTTNRINSLTIFAG-QVSGQIPALVGDLPYLETLEFHKQPNLTGPIQPAIAKLKGLKSL 125

Query: 126 DLSWNTLTGSIPNFLSSLTNLELLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGPIP 185
            LSW  L+GS+P+FLS L NL  LDLSFN LTG+IPSSL+ L  LG L LDRNKLTG IP
Sbjct: 126 RLSWTNLSGSVPDFLSQLKNLTFLDLSFNNLTGAIPSSLSELPNLGALRLDRNKLTGHIP 185

Query: 186 DSFGNFKGKIPYLYLSHNQLSGKIPTSFERVDFNVIDLSRNRLEGDGSVIFGGKKTTEIV 245
            SFG F G +P LYLSHNQLSG IPTSF ++DF  IDLSRN+LEGD SVIFG  KTT+IV
Sbjct: 186 ISFGQFIGNVPDLYLSHNQLSGNIPTSFAQMDFTSIDLSRNKLEGDASVIFGLNKTTQIV 245

Query: 246 DLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGKIPVMAVNLQLQLLNVSYNKLCGRIPVG 305
           DLSRNLLEF++SKV  P SLT LD+NHNKI+G IPV    L  Q LNVSYN+LCG+IPVG
Sbjct: 246 DLSRNLLEFNLSKVEFPTSLTSLDINHNKIYGSIPVEFTQLNFQFLNVSYNRLCGQIPVG 305

Query: 306 GKLQSFDVYSYSNNKCLCGKPLGKCK 329
           GKLQSFD YSY +N+CLCG PL  CK
Sbjct: 306 GKLQSFDEYSYFHNRCLCGAPLPSCK 330

BLAST of CmaCh03G000780.1 vs. ExPASy Swiss-Prot
Match: A7PW81 (Polygalacturonase inhibitor OS=Vitis vinifera OX=29760 GN=pgip PE=1 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 1.2e-116
Identity = 203/310 (65.48%), Postives = 234/310 (75.48%), Query Frame = 0

Query: 19  SFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCVECDRNSHRIVSLTIFADT 78
           S +E C+P DKK LL IKKA +NPYIL SW P  DCC WYCVECD  +HRI SLTIF+  
Sbjct: 25  SLSERCNPKDKKVLLQIKKALDNPYILASWNPNTDCCGWYCVECDLTTHRINSLTIFSG- 84

Query: 79  RLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLKYLDLSWNTLTGSIPNFLS 138
           +LSGQIP  VGDLP+LE L+F KL NLTG I PAIAKL +LK + LSW  L+G +P F S
Sbjct: 85  QLSGQIPDAVGDLPFLETLIFRKLSNLTGQIPPAIAKLKHLKMVRLSWTNLSGPVPAFFS 144

Query: 139 SLTNLELLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGPIPDSFGNFKGKIPYLYLS 198
            L NL  LDLSFN L+G IP SL+ L  LG LHLDRN LTGPIPDSFG F G  P L+LS
Sbjct: 145 ELKNLTYLDLSFNNLSGPIPGSLSLLPNLGALHLDRNHLTGPIPDSFGKFAGSTPGLHLS 204

Query: 199 HNQLSGKIPTSFERVDFNVIDLSRNRLEGDGSVIFGGKKTTEIVDLSRNLLEFDMSKVVL 258
           HNQLSGKIP SF   D NV+DLSRN+LEGD S+ F   K+T+IVD SRNL +FD+S+V  
Sbjct: 205 HNQLSGKIPYSFRGFDPNVMDLSRNKLEGDLSIFFNANKSTQIVDFSRNLFQFDLSRVEF 264

Query: 259 PRSLTWLDLNHNKIFGKIPVMAVNLQLQLLNVSYNKLCGRIPVGGKLQSFDVYSYSNNKC 318
           P+SLT LDL+HNKI G +P M  +L LQ LNVSYN+LCG+IPVGGKLQSFD  SY +N+C
Sbjct: 265 PKSLTSLDLSHNKIAGSLPEMMTSLDLQFLNVSYNRLCGKIPVGGKLQSFDYDSYFHNRC 324

Query: 319 LCGKPLGKCK 329
           LCG PL  CK
Sbjct: 325 LCGAPLQSCK 333

BLAST of CmaCh03G000780.1 vs. ExPASy Swiss-Prot
Match: Q9M5J9 (Polygalacturonase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PGIP1 PE=1 SV=1)

HSP 1 Score: 404.1 bits (1037), Expect = 1.6e-111
Identity = 199/331 (60.12%), Postives = 240/331 (72.51%), Query Frame = 0

Query: 1   MSSSPSTFLLIFFLFFTISFA-ELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYC 60
           M  + +  LL  F F T   + +LC+ NDK  LL IKK+ NNPY L SW P+ DCC+WYC
Sbjct: 1   MDKTATLCLLFLFTFLTTCLSKDLCNQNDKNTLLKIKKSLNNPYHLASWDPQTDCCSWYC 60

Query: 61  VECD--RNSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLH 120
           +EC     +HR+ +LTIF+  ++SGQIP  VGDLPYLE L+F KL NLTG IQP IAKL 
Sbjct: 61  LECGDATVNHRVTALTIFSG-QISGQIPAEVGDLPYLETLVFRKLSNLTGTIQPTIAKLK 120

Query: 121 NLKYLDLSWNTLTGSIPNFLSSLTNLELLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKL 180
           NL+ L LSW  LTG IP+F+S L NLE L+LSFN L+GSIPSSL+ L ++  L L RNKL
Sbjct: 121 NLRMLRLSWTNLTGPIPDFISQLKNLEFLELSFNDLSGSIPSSLSTLPKILALELSRNKL 180

Query: 181 TGPIPDSFGNFKGKIPYLYLSHNQLSGKIPTSFERVDFNVIDLSRNRLEGDGSVIFGGKK 240
           TG IP+SFG+F G +P L LSHNQLSG IP S   +DFN IDLSRN+L+GD S++FG  K
Sbjct: 181 TGSIPESFGSFPGTVPDLRLSHNQLSGPIPKSLGNIDFNRIDLSRNKLQGDASMLFGSNK 240

Query: 241 TTEIVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGKIPVMAVNLQLQLLNVSYNKLCG 300
           TT  +DLSRN+ +FD+SKV +P++L  LDLNHN I G IPV      LQ  NVSYNKLCG
Sbjct: 241 TTWSIDLSRNMFQFDISKVDIPKTLGILDLNHNGITGNIPVQWTEAPLQFFNVSYNKLCG 300

Query: 301 RIPVGGKLQSFDVYSYSNNKCLCGKPLGKCK 329
            IP GGKLQ+FD YSY +NKCLCG PL  CK
Sbjct: 301 HIPTGGKLQTFDSYSYFHNKCLCGAPLEICK 330

BLAST of CmaCh03G000780.1 vs. ExPASy Swiss-Prot
Match: Q9M5J8 (Polygalacturonase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PGIP2 PE=2 SV=2)

HSP 1 Score: 404.1 bits (1037), Expect = 1.6e-111
Identity = 200/331 (60.42%), Postives = 240/331 (72.51%), Query Frame = 0

Query: 1   MSSSPSTFLLIFFLFFTISFA-ELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYC 60
           M  + + FLL+  L  T S A +LCH +DK  LL IKK+ NNPY L SW P+ DCC+WYC
Sbjct: 1   MDKTMTLFLLLSTLLLTTSLAKDLCHKDDKTTLLKIKKSLNNPYHLASWDPKTDCCSWYC 60

Query: 61  VECD--RNSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLH 120
           +EC     +HR+ SL I  D  +SGQIPP VGDLPYL +L+F KL NLTG IQP IAKL 
Sbjct: 61  LECGDATVNHRVTSL-IIQDGEISGQIPPEVGDLPYLTSLIFRKLTNLTGHIQPTIAKLK 120

Query: 121 NLKYLDLSWNTLTGSIPNFLSSLTNLELLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKL 180
           NL +L LSW  LTG +P FLS L NLE +DLSFN L+GSIPSSL++L++L  L L RNKL
Sbjct: 121 NLTFLRLSWTNLTGPVPEFLSQLKNLEYIDLSFNDLSGSIPSSLSSLRKLEYLELSRNKL 180

Query: 181 TGPIPDSFGNFKGKIPYLYLSHNQLSGKIPTSFERVDFNVIDLSRNRLEGDGSVIFGGKK 240
           TGPIP+SFG F GK+P L+LSHNQLSG IP S    DF  IDLSRN+L+GD S++FG KK
Sbjct: 181 TGPIPESFGTFSGKVPSLFLSHNQLSGTIPKSLGNPDFYRIDLSRNKLQGDASILFGAKK 240

Query: 241 TTEIVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGKIPVMAVNLQLQLLNVSYNKLCG 300
           TT IVD+SRN+ +FD+SKV L ++L  LD+NHN I G IP        QLLNVSYN+LCG
Sbjct: 241 TTWIVDISRNMFQFDLSKVKLAKTLNNLDMNHNGITGSIPAEWSKAYFQLLNVSYNRLCG 300

Query: 301 RIPVGGKLQSFDVYSYSNNKCLCGKPLGKCK 329
           RIP G  +Q FD YS+ +NKCLCG PL  CK
Sbjct: 301 RIPKGEYIQRFDSYSFFHNKCLCGAPLPSCK 330

BLAST of CmaCh03G000780.1 vs. ExPASy Swiss-Prot
Match: Q9LH52 (Leucine-rich repeat protein FLOR 1 OS=Arabidopsis thaliana OX=3702 GN=FLR1 PE=1 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 1.5e-82
Identity = 162/322 (50.31%), Postives = 207/322 (64.29%), Query Frame = 0

Query: 9   LLIFF--LFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCT-WYCVECDRN 68
           L IFF  LF T+  +  C  NDK ALL IKKA  NP +L+SW P  DCCT W  VEC   
Sbjct: 7   LSIFFSILFITLPSSYSCTENDKNALLQIKKALGNPPLLSSWNPRTDCCTGWTGVEC--T 66

Query: 69  SHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLKYLDLS 128
           + R+  L++     +SGQI   +GDL  L  L F  LP+LTG I   I KL NL  L L 
Sbjct: 67  NRRVTGLSV-TSGEVSGQISYQIGDLVDLRTLDFSYLPHLTGNIPRTITKLKNLNTLYLK 126

Query: 129 WNTLTGSIPNFLSSLTNLELLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGPIPDSF 188
             +L+G IP+++S L +L  LDLSFN+ TG IP SL+ + +L  + ++ NKLTG IP+SF
Sbjct: 127 HTSLSGPIPDYISELKSLTFLDLSFNQFTGPIPGSLSQMPKLEAIQINDNKLTGSIPNSF 186

Query: 189 GNFKGKIPYLYLSHNQLSGKIPTSFERVDFNVIDLSRNRLEGDGSVIFGGKKTTEIVDLS 248
           G+F G +P LYLS+N+LSGKIP S  + DFN +DLS N  EGD  + FG  KTT  VDLS
Sbjct: 187 GSFVGNVPNLYLSNNKLSGKIPESLSKYDFNAVDLSGNGFEGDAFMFFGRNKTTVRVDLS 246

Query: 249 RNLLEFDMSKVVLPRSLTWLDLNHNKIFGKIPVMAVNLQLQLLNVSYNKLCGRIPVGGKL 308
           RN+  FD+ KV   RS+  LDL+ N I+GKIP     L L+  NVS N LCG+IP GG L
Sbjct: 247 RNMFNFDLVKVKFARSIVSLDLSQNHIYGKIPPALTKLHLEHFNVSDNHLCGKIPSGGLL 306

Query: 309 QSFDVYSYSNNKCLCGKPLGKC 328
           Q+F+  ++++N CLCG PL  C
Sbjct: 307 QTFEPSAFAHNICLCGTPLKAC 325

BLAST of CmaCh03G000780.1 vs. ExPASy TrEMBL
Match: A0A6J1HW96 (polygalacturonase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111467229 PE=4 SV=1)

HSP 1 Score: 681.0 bits (1756), Expect = 2.4e-192
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 0

Query: 1   MSSSPSTFLLIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCV 60
           MSSSPSTFLLIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCV
Sbjct: 1   MSSSPSTFLLIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCV 60

Query: 61  ECDRNSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLK 120
           ECDRNSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLK
Sbjct: 61  ECDRNSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLK 120

Query: 121 YLDLSWNTLTGSIPNFLSSLTNLELLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGP 180
           YLDLSWNTLTGSIPNFLSSLTNLELLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGP
Sbjct: 121 YLDLSWNTLTGSIPNFLSSLTNLELLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGP 180

Query: 181 IPDSFGNFKGKIPYLYLSHNQLSGKIPTSFERVDFNVIDLSRNRLEGDGSVIFGGKKTTE 240
           IPDSFGNFKGKIPYLYLSHNQLSGKIPTSFERVDFNVIDLSRNRLEGDGSVIFGGKKTTE
Sbjct: 181 IPDSFGNFKGKIPYLYLSHNQLSGKIPTSFERVDFNVIDLSRNRLEGDGSVIFGGKKTTE 240

Query: 241 IVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGKIPVMAVNLQLQLLNVSYNKLCGRIP 300
           IVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGKIPVMAVNLQLQLLNVSYNKLCGRIP
Sbjct: 241 IVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGKIPVMAVNLQLQLLNVSYNKLCGRIP 300

Query: 301 VGGKLQSFDVYSYSNNKCLCGKPLGKCK 329
           VGGKLQSFDVYSYSNNKCLCGKPLGKCK
Sbjct: 301 VGGKLQSFDVYSYSNNKCLCGKPLGKCK 328

BLAST of CmaCh03G000780.1 vs. ExPASy TrEMBL
Match: A0A6J1ELI8 (polygalacturonase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111435518 PE=4 SV=1)

HSP 1 Score: 670.6 bits (1729), Expect = 3.3e-189
Identity = 322/328 (98.17%), Postives = 325/328 (99.09%), Query Frame = 0

Query: 1   MSSSPSTFLLIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCV 60
           MSSSPSTFL IFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCV
Sbjct: 1   MSSSPSTFLFIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCV 60

Query: 61  ECDRNSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLK 120
           ECDR SHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLK
Sbjct: 61  ECDRKSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLK 120

Query: 121 YLDLSWNTLTGSIPNFLSSLTNLELLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGP 180
           YLDLSWN+LTGSIPNFLSSLTNL+LLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGP
Sbjct: 121 YLDLSWNSLTGSIPNFLSSLTNLDLLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGP 180

Query: 181 IPDSFGNFKGKIPYLYLSHNQLSGKIPTSFERVDFNVIDLSRNRLEGDGSVIFGGKKTTE 240
           IPDSFGNFKGKIPYLYLSHNQLSGKIPTS ERVDFNVIDLSRNRLEGDGSVIFGGKKTTE
Sbjct: 181 IPDSFGNFKGKIPYLYLSHNQLSGKIPTSLERVDFNVIDLSRNRLEGDGSVIFGGKKTTE 240

Query: 241 IVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGKIPVMAVNLQLQLLNVSYNKLCGRIP 300
           IVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFG+IPVMAVNLQLQLLNVSYNKLCGRIP
Sbjct: 241 IVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGEIPVMAVNLQLQLLNVSYNKLCGRIP 300

Query: 301 VGGKLQSFDVYSYSNNKCLCGKPLGKCK 329
           VGGKLQSFDVYSYSNNKCLCGKPLGKCK
Sbjct: 301 VGGKLQSFDVYSYSNNKCLCGKPLGKCK 328

BLAST of CmaCh03G000780.1 vs. ExPASy TrEMBL
Match: Q7X9Q7 (Polygalacturonase-inhibiting protein OS=Cucumis melo OX=3656 GN=LOC103499550 PE=2 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 7.6e-162
Identity = 269/320 (84.06%), Postives = 293/320 (91.56%), Query Frame = 0

Query: 9   LLIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCVECDRNSHR 68
           LL+ F FFT+SFAELCHPNDK+ LLNIKKAFNNPYILTSWKPEEDCCTWYCVECD  SHR
Sbjct: 7   LLLLFFFFTVSFAELCHPNDKEVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECDLKSHR 66

Query: 69  IVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLKYLDLSWNT 128
           I++LTIFAD  LSG IPPFVGDLP+LENLMFHKLPNLTGPI P IAKLHNLKYLDLSWN 
Sbjct: 67  IIALTIFADDELSGPIPPFVGDLPFLENLMFHKLPNLTGPIPPTIAKLHNLKYLDLSWNG 126

Query: 129 LTGSIPNFLSSLTNLELLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGPIPDSFGNF 188
           L+G IP+FL SL+NL++LDLSFNR TGSIPSSLANL+RLGTLHLDRNKLTGPIPDSFGNF
Sbjct: 127 LSGPIPSFLGSLSNLDILDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPDSFGNF 186

Query: 189 KGKIPYLYLSHNQLSGKIPTSFERVDFNVIDLSRNRLEGDGSVIFGGKKTTEIVDLSRNL 248
           KGK+PYLYLSHNQLSGKIPTS  +VDFN IDLSRN+L GDGS+IFG KKTTEIVDLSRNL
Sbjct: 187 KGKVPYLYLSHNQLSGKIPTSMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIVDLSRNL 246

Query: 249 LEFDMSKVVLPRSLTWLDLNHNKIFGKIPVMAVNLQLQLLNVSYNKLCGRIPVGGKLQSF 308
           LEF++SKVV PR+LTWLDLNHNKIFG+IP   V L+LQ+LNVSYN LCGRIP+GGKLQSF
Sbjct: 247 LEFNISKVVFPRTLTWLDLNHNKIFGEIPTGVVKLELQMLNVSYNALCGRIPMGGKLQSF 306

Query: 309 DVYSYSNNKCLCGKPLGKCK 329
           DVYSY +NKCLCGKPLG CK
Sbjct: 307 DVYSYFHNKCLCGKPLGSCK 326

BLAST of CmaCh03G000780.1 vs. ExPASy TrEMBL
Match: A0A5D3BX47 (Polygalacturonase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold4559G00050 PE=4 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 7.6e-162
Identity = 269/320 (84.06%), Postives = 293/320 (91.56%), Query Frame = 0

Query: 9   LLIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCVECDRNSHR 68
           LL+ F FFT+SFAELCHPNDK+ LLNIKKAFNNPYILTSWKPEEDCCTWYCVECD  SHR
Sbjct: 7   LLLLFFFFTVSFAELCHPNDKEVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECDLKSHR 66

Query: 69  IVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLKYLDLSWNT 128
           I++LTIFAD  LSG IPPFVGDLP+LENLMFHKLPNLTGPI P IAKLHNLKYLDLSWN 
Sbjct: 67  IIALTIFADDELSGPIPPFVGDLPFLENLMFHKLPNLTGPIPPTIAKLHNLKYLDLSWNG 126

Query: 129 LTGSIPNFLSSLTNLELLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGPIPDSFGNF 188
           L+G IP+FL SL+NL++LDLSFNR TGSIPSSLANL+RLGTLHLDRNKLTGPIPDSFGNF
Sbjct: 127 LSGPIPSFLGSLSNLDILDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPDSFGNF 186

Query: 189 KGKIPYLYLSHNQLSGKIPTSFERVDFNVIDLSRNRLEGDGSVIFGGKKTTEIVDLSRNL 248
           KGK+PYLYLSHNQLSGKIPTS  +VDFN IDLSRN+L GDGS+IFG KKTTEIVDLSRNL
Sbjct: 187 KGKVPYLYLSHNQLSGKIPTSMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIVDLSRNL 246

Query: 249 LEFDMSKVVLPRSLTWLDLNHNKIFGKIPVMAVNLQLQLLNVSYNKLCGRIPVGGKLQSF 308
           LEF++SKVV PR+LTWLDLNHNKIFG+IP   V L+LQ+LNVSYN LCGRIP+GGKLQSF
Sbjct: 247 LEFNISKVVFPRTLTWLDLNHNKIFGEIPTGVVKLELQMLNVSYNALCGRIPMGGKLQSF 306

Query: 309 DVYSYSNNKCLCGKPLGKCK 329
           DVYSY +NKCLCGKPLG CK
Sbjct: 307 DVYSYFHNKCLCGKPLGSCK 326

BLAST of CmaCh03G000780.1 vs. ExPASy TrEMBL
Match: K9MZG2 (Polygalacturonase-inhibiting protein OS=Cucumis melo subsp. agrestis OX=217619 GN=pgip PE=4 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 1.7e-161
Identity = 269/320 (84.06%), Postives = 292/320 (91.25%), Query Frame = 0

Query: 9   LLIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCVECDRNSHR 68
           LL+ F FFT+SFAELCHPNDK+ LLNIKKAFNNPYILTSWKPEEDCCTWYCVECD  SHR
Sbjct: 7   LLLLFFFFTVSFAELCHPNDKEVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECDLKSHR 66

Query: 69  IVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLKYLDLSWNT 128
           I +LTIFAD  LSG IPPFVGDLP+LENLMFHKLPNLTGPI P IAKLHNLKYLDLSWN 
Sbjct: 67  ITALTIFADDELSGPIPPFVGDLPFLENLMFHKLPNLTGPIPPTIAKLHNLKYLDLSWNG 126

Query: 129 LTGSIPNFLSSLTNLELLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGPIPDSFGNF 188
           L+G IP+FL SL+NL++LDLSFNR TGSIPSSLANL+RLGTLHLDRNKLTGPIPDSFGNF
Sbjct: 127 LSGPIPSFLGSLSNLDILDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPDSFGNF 186

Query: 189 KGKIPYLYLSHNQLSGKIPTSFERVDFNVIDLSRNRLEGDGSVIFGGKKTTEIVDLSRNL 248
           KGK+PYLYLSHNQLSGKIPTS  +VDFN IDLSRN+L GDGS+IFG KKTTEIVDLSRNL
Sbjct: 187 KGKVPYLYLSHNQLSGKIPTSMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIVDLSRNL 246

Query: 249 LEFDMSKVVLPRSLTWLDLNHNKIFGKIPVMAVNLQLQLLNVSYNKLCGRIPVGGKLQSF 308
           LEF++SKVV PR+LTWLDLNHNKIFG+IP   V L+LQ+LNVSYN LCGRIP+GGKLQSF
Sbjct: 247 LEFNISKVVFPRTLTWLDLNHNKIFGEIPTGVVKLELQMLNVSYNALCGRIPMGGKLQSF 306

Query: 309 DVYSYSNNKCLCGKPLGKCK 329
           DVYSY +NKCLCGKPLG CK
Sbjct: 307 DVYSYFHNKCLCGKPLGSCK 326

BLAST of CmaCh03G000780.1 vs. NCBI nr
Match: XP_022967838.1 (polygalacturonase inhibitor-like [Cucurbita maxima])

HSP 1 Score: 681.0 bits (1756), Expect = 5.0e-192
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 0

Query: 1   MSSSPSTFLLIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCV 60
           MSSSPSTFLLIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCV
Sbjct: 1   MSSSPSTFLLIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCV 60

Query: 61  ECDRNSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLK 120
           ECDRNSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLK
Sbjct: 61  ECDRNSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLK 120

Query: 121 YLDLSWNTLTGSIPNFLSSLTNLELLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGP 180
           YLDLSWNTLTGSIPNFLSSLTNLELLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGP
Sbjct: 121 YLDLSWNTLTGSIPNFLSSLTNLELLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGP 180

Query: 181 IPDSFGNFKGKIPYLYLSHNQLSGKIPTSFERVDFNVIDLSRNRLEGDGSVIFGGKKTTE 240
           IPDSFGNFKGKIPYLYLSHNQLSGKIPTSFERVDFNVIDLSRNRLEGDGSVIFGGKKTTE
Sbjct: 181 IPDSFGNFKGKIPYLYLSHNQLSGKIPTSFERVDFNVIDLSRNRLEGDGSVIFGGKKTTE 240

Query: 241 IVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGKIPVMAVNLQLQLLNVSYNKLCGRIP 300
           IVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGKIPVMAVNLQLQLLNVSYNKLCGRIP
Sbjct: 241 IVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGKIPVMAVNLQLQLLNVSYNKLCGRIP 300

Query: 301 VGGKLQSFDVYSYSNNKCLCGKPLGKCK 329
           VGGKLQSFDVYSYSNNKCLCGKPLGKCK
Sbjct: 301 VGGKLQSFDVYSYSNNKCLCGKPLGKCK 328

BLAST of CmaCh03G000780.1 vs. NCBI nr
Match: XP_022928674.1 (polygalacturonase inhibitor-like [Cucurbita moschata] >XP_023543570.1 polygalacturonase inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 670.6 bits (1729), Expect = 6.8e-189
Identity = 322/328 (98.17%), Postives = 325/328 (99.09%), Query Frame = 0

Query: 1   MSSSPSTFLLIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCV 60
           MSSSPSTFL IFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCV
Sbjct: 1   MSSSPSTFLFIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCV 60

Query: 61  ECDRNSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLK 120
           ECDR SHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLK
Sbjct: 61  ECDRKSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLK 120

Query: 121 YLDLSWNTLTGSIPNFLSSLTNLELLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGP 180
           YLDLSWN+LTGSIPNFLSSLTNL+LLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGP
Sbjct: 121 YLDLSWNSLTGSIPNFLSSLTNLDLLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGP 180

Query: 181 IPDSFGNFKGKIPYLYLSHNQLSGKIPTSFERVDFNVIDLSRNRLEGDGSVIFGGKKTTE 240
           IPDSFGNFKGKIPYLYLSHNQLSGKIPTS ERVDFNVIDLSRNRLEGDGSVIFGGKKTTE
Sbjct: 181 IPDSFGNFKGKIPYLYLSHNQLSGKIPTSLERVDFNVIDLSRNRLEGDGSVIFGGKKTTE 240

Query: 241 IVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGKIPVMAVNLQLQLLNVSYNKLCGRIP 300
           IVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFG+IPVMAVNLQLQLLNVSYNKLCGRIP
Sbjct: 241 IVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGEIPVMAVNLQLQLLNVSYNKLCGRIP 300

Query: 301 VGGKLQSFDVYSYSNNKCLCGKPLGKCK 329
           VGGKLQSFDVYSYSNNKCLCGKPLGKCK
Sbjct: 301 VGGKLQSFDVYSYSNNKCLCGKPLGKCK 328

BLAST of CmaCh03G000780.1 vs. NCBI nr
Match: KAG6603233.1 (Polygalacturonase inhibitor, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 668.3 bits (1723), Expect = 3.4e-188
Identity = 320/328 (97.56%), Postives = 325/328 (99.09%), Query Frame = 0

Query: 1   MSSSPSTFLLIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCV 60
           MSSSPSTFL IFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCV
Sbjct: 1   MSSSPSTFLFIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCV 60

Query: 61  ECDRNSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLK 120
           +CDR SHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLK
Sbjct: 61  KCDRKSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLK 120

Query: 121 YLDLSWNTLTGSIPNFLSSLTNLELLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGP 180
           YLDLSWN+LTGSIPNFLSSLTNL++LDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGP
Sbjct: 121 YLDLSWNSLTGSIPNFLSSLTNLDILDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGP 180

Query: 181 IPDSFGNFKGKIPYLYLSHNQLSGKIPTSFERVDFNVIDLSRNRLEGDGSVIFGGKKTTE 240
           IPDSFGNFKGKIPYLYLSHNQLSGKIPTS ERVDFNVIDLSRNRLEGDGSVIFGGKKTTE
Sbjct: 181 IPDSFGNFKGKIPYLYLSHNQLSGKIPTSLERVDFNVIDLSRNRLEGDGSVIFGGKKTTE 240

Query: 241 IVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGKIPVMAVNLQLQLLNVSYNKLCGRIP 300
           IVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFG+IPVMAVNLQLQLLNVSYNKLCGRIP
Sbjct: 241 IVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGEIPVMAVNLQLQLLNVSYNKLCGRIP 300

Query: 301 VGGKLQSFDVYSYSNNKCLCGKPLGKCK 329
           VGGKLQSFDVYSYSNNKCLCGKPLGKCK
Sbjct: 301 VGGKLQSFDVYSYSNNKCLCGKPLGKCK 328

BLAST of CmaCh03G000780.1 vs. NCBI nr
Match: NP_001303611.1 (polygalacturonase inhibitor-like precursor [Cucumis melo] >AAP41199.1 polygalacturonase-inhibiting protein [Cucumis melo] >KAA0053950.1 polygalacturonase inhibitor-like precursor [Cucumis melo var. makuwa] >TYK04261.1 polygalacturonase inhibitor-like precursor [Cucumis melo var. makuwa])

HSP 1 Score: 579.7 bits (1493), Expect = 1.6e-161
Identity = 269/320 (84.06%), Postives = 293/320 (91.56%), Query Frame = 0

Query: 9   LLIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCVECDRNSHR 68
           LL+ F FFT+SFAELCHPNDK+ LLNIKKAFNNPYILTSWKPEEDCCTWYCVECD  SHR
Sbjct: 7   LLLLFFFFTVSFAELCHPNDKEVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECDLKSHR 66

Query: 69  IVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLKYLDLSWNT 128
           I++LTIFAD  LSG IPPFVGDLP+LENLMFHKLPNLTGPI P IAKLHNLKYLDLSWN 
Sbjct: 67  IIALTIFADDELSGPIPPFVGDLPFLENLMFHKLPNLTGPIPPTIAKLHNLKYLDLSWNG 126

Query: 129 LTGSIPNFLSSLTNLELLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGPIPDSFGNF 188
           L+G IP+FL SL+NL++LDLSFNR TGSIPSSLANL+RLGTLHLDRNKLTGPIPDSFGNF
Sbjct: 127 LSGPIPSFLGSLSNLDILDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPDSFGNF 186

Query: 189 KGKIPYLYLSHNQLSGKIPTSFERVDFNVIDLSRNRLEGDGSVIFGGKKTTEIVDLSRNL 248
           KGK+PYLYLSHNQLSGKIPTS  +VDFN IDLSRN+L GDGS+IFG KKTTEIVDLSRNL
Sbjct: 187 KGKVPYLYLSHNQLSGKIPTSMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIVDLSRNL 246

Query: 249 LEFDMSKVVLPRSLTWLDLNHNKIFGKIPVMAVNLQLQLLNVSYNKLCGRIPVGGKLQSF 308
           LEF++SKVV PR+LTWLDLNHNKIFG+IP   V L+LQ+LNVSYN LCGRIP+GGKLQSF
Sbjct: 247 LEFNISKVVFPRTLTWLDLNHNKIFGEIPTGVVKLELQMLNVSYNALCGRIPMGGKLQSF 306

Query: 309 DVYSYSNNKCLCGKPLGKCK 329
           DVYSY +NKCLCGKPLG CK
Sbjct: 307 DVYSYFHNKCLCGKPLGSCK 326

BLAST of CmaCh03G000780.1 vs. NCBI nr
Match: AFY10521.1 (polygalacturonase-inhibiting protein [Cucumis melo subsp. agrestis])

HSP 1 Score: 578.6 bits (1490), Expect = 3.5e-161
Identity = 269/320 (84.06%), Postives = 292/320 (91.25%), Query Frame = 0

Query: 9   LLIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCVECDRNSHR 68
           LL+ F FFT+SFAELCHPNDK+ LLNIKKAFNNPYILTSWKPEEDCCTWYCVECD  SHR
Sbjct: 7   LLLLFFFFTVSFAELCHPNDKEVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECDLKSHR 66

Query: 69  IVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLKYLDLSWNT 128
           I +LTIFAD  LSG IPPFVGDLP+LENLMFHKLPNLTGPI P IAKLHNLKYLDLSWN 
Sbjct: 67  ITALTIFADDELSGPIPPFVGDLPFLENLMFHKLPNLTGPIPPTIAKLHNLKYLDLSWNG 126

Query: 129 LTGSIPNFLSSLTNLELLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGPIPDSFGNF 188
           L+G IP+FL SL+NL++LDLSFNR TGSIPSSLANL+RLGTLHLDRNKLTGPIPDSFGNF
Sbjct: 127 LSGPIPSFLGSLSNLDILDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPDSFGNF 186

Query: 189 KGKIPYLYLSHNQLSGKIPTSFERVDFNVIDLSRNRLEGDGSVIFGGKKTTEIVDLSRNL 248
           KGK+PYLYLSHNQLSGKIPTS  +VDFN IDLSRN+L GDGS+IFG KKTTEIVDLSRNL
Sbjct: 187 KGKVPYLYLSHNQLSGKIPTSMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIVDLSRNL 246

Query: 249 LEFDMSKVVLPRSLTWLDLNHNKIFGKIPVMAVNLQLQLLNVSYNKLCGRIPVGGKLQSF 308
           LEF++SKVV PR+LTWLDLNHNKIFG+IP   V L+LQ+LNVSYN LCGRIP+GGKLQSF
Sbjct: 247 LEFNISKVVFPRTLTWLDLNHNKIFGEIPTGVVKLELQMLNVSYNALCGRIPMGGKLQSF 306

Query: 309 DVYSYSNNKCLCGKPLGKCK 329
           DVYSY +NKCLCGKPLG CK
Sbjct: 307 DVYSYFHNKCLCGKPLGSCK 326

BLAST of CmaCh03G000780.1 vs. TAIR 10
Match: AT5G06860.1 (polygalacturonase inhibiting protein 1 )

HSP 1 Score: 404.1 bits (1037), Expect = 1.1e-112
Identity = 199/331 (60.12%), Postives = 240/331 (72.51%), Query Frame = 0

Query: 1   MSSSPSTFLLIFFLFFTISFA-ELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYC 60
           M  + +  LL  F F T   + +LC+ NDK  LL IKK+ NNPY L SW P+ DCC+WYC
Sbjct: 1   MDKTATLCLLFLFTFLTTCLSKDLCNQNDKNTLLKIKKSLNNPYHLASWDPQTDCCSWYC 60

Query: 61  VECD--RNSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLH 120
           +EC     +HR+ +LTIF+  ++SGQIP  VGDLPYLE L+F KL NLTG IQP IAKL 
Sbjct: 61  LECGDATVNHRVTALTIFSG-QISGQIPAEVGDLPYLETLVFRKLSNLTGTIQPTIAKLK 120

Query: 121 NLKYLDLSWNTLTGSIPNFLSSLTNLELLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKL 180
           NL+ L LSW  LTG IP+F+S L NLE L+LSFN L+GSIPSSL+ L ++  L L RNKL
Sbjct: 121 NLRMLRLSWTNLTGPIPDFISQLKNLEFLELSFNDLSGSIPSSLSTLPKILALELSRNKL 180

Query: 181 TGPIPDSFGNFKGKIPYLYLSHNQLSGKIPTSFERVDFNVIDLSRNRLEGDGSVIFGGKK 240
           TG IP+SFG+F G +P L LSHNQLSG IP S   +DFN IDLSRN+L+GD S++FG  K
Sbjct: 181 TGSIPESFGSFPGTVPDLRLSHNQLSGPIPKSLGNIDFNRIDLSRNKLQGDASMLFGSNK 240

Query: 241 TTEIVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGKIPVMAVNLQLQLLNVSYNKLCG 300
           TT  +DLSRN+ +FD+SKV +P++L  LDLNHN I G IPV      LQ  NVSYNKLCG
Sbjct: 241 TTWSIDLSRNMFQFDISKVDIPKTLGILDLNHNGITGNIPVQWTEAPLQFFNVSYNKLCG 300

Query: 301 RIPVGGKLQSFDVYSYSNNKCLCGKPLGKCK 329
            IP GGKLQ+FD YSY +NKCLCG PL  CK
Sbjct: 301 HIPTGGKLQTFDSYSYFHNKCLCGAPLEICK 330

BLAST of CmaCh03G000780.1 vs. TAIR 10
Match: AT5G06870.1 (polygalacturonase inhibiting protein 2 )

HSP 1 Score: 404.1 bits (1037), Expect = 1.1e-112
Identity = 200/331 (60.42%), Postives = 240/331 (72.51%), Query Frame = 0

Query: 1   MSSSPSTFLLIFFLFFTISFA-ELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYC 60
           M  + + FLL+  L  T S A +LCH +DK  LL IKK+ NNPY L SW P+ DCC+WYC
Sbjct: 1   MDKTMTLFLLLSTLLLTTSLAKDLCHKDDKTTLLKIKKSLNNPYHLASWDPKTDCCSWYC 60

Query: 61  VECD--RNSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLH 120
           +EC     +HR+ SL I  D  +SGQIPP VGDLPYL +L+F KL NLTG IQP IAKL 
Sbjct: 61  LECGDATVNHRVTSL-IIQDGEISGQIPPEVGDLPYLTSLIFRKLTNLTGHIQPTIAKLK 120

Query: 121 NLKYLDLSWNTLTGSIPNFLSSLTNLELLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKL 180
           NL +L LSW  LTG +P FLS L NLE +DLSFN L+GSIPSSL++L++L  L L RNKL
Sbjct: 121 NLTFLRLSWTNLTGPVPEFLSQLKNLEYIDLSFNDLSGSIPSSLSSLRKLEYLELSRNKL 180

Query: 181 TGPIPDSFGNFKGKIPYLYLSHNQLSGKIPTSFERVDFNVIDLSRNRLEGDGSVIFGGKK 240
           TGPIP+SFG F GK+P L+LSHNQLSG IP S    DF  IDLSRN+L+GD S++FG KK
Sbjct: 181 TGPIPESFGTFSGKVPSLFLSHNQLSGTIPKSLGNPDFYRIDLSRNKLQGDASILFGAKK 240

Query: 241 TTEIVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGKIPVMAVNLQLQLLNVSYNKLCG 300
           TT IVD+SRN+ +FD+SKV L ++L  LD+NHN I G IP        QLLNVSYN+LCG
Sbjct: 241 TTWIVDISRNMFQFDLSKVKLAKTLNNLDMNHNGITGSIPAEWSKAYFQLLNVSYNRLCG 300

Query: 301 RIPVGGKLQSFDVYSYSNNKCLCGKPLGKCK 329
           RIP G  +Q FD YS+ +NKCLCG PL  CK
Sbjct: 301 RIPKGEYIQRFDSYSFFHNKCLCGAPLPSCK 330

BLAST of CmaCh03G000780.1 vs. TAIR 10
Match: AT3G12145.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 307.8 bits (787), Expect = 1.1e-83
Identity = 162/322 (50.31%), Postives = 207/322 (64.29%), Query Frame = 0

Query: 9   LLIFF--LFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCT-WYCVECDRN 68
           L IFF  LF T+  +  C  NDK ALL IKKA  NP +L+SW P  DCCT W  VEC   
Sbjct: 7   LSIFFSILFITLPSSYSCTENDKNALLQIKKALGNPPLLSSWNPRTDCCTGWTGVEC--T 66

Query: 69  SHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLKYLDLS 128
           + R+  L++     +SGQI   +GDL  L  L F  LP+LTG I   I KL NL  L L 
Sbjct: 67  NRRVTGLSV-TSGEVSGQISYQIGDLVDLRTLDFSYLPHLTGNIPRTITKLKNLNTLYLK 126

Query: 129 WNTLTGSIPNFLSSLTNLELLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGPIPDSF 188
             +L+G IP+++S L +L  LDLSFN+ TG IP SL+ + +L  + ++ NKLTG IP+SF
Sbjct: 127 HTSLSGPIPDYISELKSLTFLDLSFNQFTGPIPGSLSQMPKLEAIQINDNKLTGSIPNSF 186

Query: 189 GNFKGKIPYLYLSHNQLSGKIPTSFERVDFNVIDLSRNRLEGDGSVIFGGKKTTEIVDLS 248
           G+F G +P LYLS+N+LSGKIP S  + DFN +DLS N  EGD  + FG  KTT  VDLS
Sbjct: 187 GSFVGNVPNLYLSNNKLSGKIPESLSKYDFNAVDLSGNGFEGDAFMFFGRNKTTVRVDLS 246

Query: 249 RNLLEFDMSKVVLPRSLTWLDLNHNKIFGKIPVMAVNLQLQLLNVSYNKLCGRIPVGGKL 308
           RN+  FD+ KV   RS+  LDL+ N I+GKIP     L L+  NVS N LCG+IP GG L
Sbjct: 247 RNMFNFDLVKVKFARSIVSLDLSQNHIYGKIPPALTKLHLEHFNVSDNHLCGKIPSGGLL 306

Query: 309 QSFDVYSYSNNKCLCGKPLGKC 328
           Q+F+  ++++N CLCG PL  C
Sbjct: 307 QTFEPSAFAHNICLCGTPLKAC 325

BLAST of CmaCh03G000780.1 vs. TAIR 10
Match: AT3G12610.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 191.4 bits (485), Expect = 1.1e-48
Identity = 121/364 (33.24%), Postives = 183/364 (50.27%), Query Frame = 0

Query: 3   SSPSTFLLIFFLFFTISFAELCHPNDKKALLNIKKAFNNPY--ILTSWKPEEDCC-TWYC 62
           +SP + LL       IS    C P D+ AL   K + + P   I  +W    DCC  WY 
Sbjct: 6   ASPFSSLLAVVFISVISVVRCCSPKDQTALNAFKSSLSEPNLGIFNTWSENTDCCKEWYG 65

Query: 63  VECDRNSHRIVSLTIFADTR------------LSGQIPPFVGDLPYLENLMFHKLPNLTG 122
           + CD +S R+  +++  ++             +SG I P V DL  L +L+      +TG
Sbjct: 66  ISCDPDSGRVTDISLRGESEDAIFQKAGRSGYMSGSIDPAVCDLTALTSLVLADWKGITG 125

Query: 123 PIQPAIAKLHNLKYLDLSWNTLTGSIPNFLSSLTNLELLDLSFNRLTGSIPSSLANLQRL 182
            I P I  L +L+ LDL+ N +TG IP  +  L+ L +L+L+ N+++G IP+SL +L  L
Sbjct: 126 EIPPCITSLASLRILDLAGNKITGEIPAEIGKLSKLAVLNLAENQMSGEIPASLTSLIEL 185

Query: 183 GTLHLDRNKLTGPIPDSFGNFKGKIPYLYLSHNQLSGKIPTSFERVD-FNVIDLSRNRLE 242
             L L  N +TG IP  FG+ K  +  + L  N+L+G IP S   ++    +DLS+N +E
Sbjct: 186 KHLELTENGITGVIPADFGSLK-MLSRVLLGRNELTGSIPESISGMERLADLDLSKNHIE 245

Query: 243 GDGSVIFGGKKT------------------------TEIVDLSRNLLEFDMSKVVLPRS- 302
           G      G  K                          ++ +LSRN LE  +  V   ++ 
Sbjct: 246 GPIPEWMGNMKVLSLLNLDCNSLTGPIPGSLLSNSGLDVANLSRNALEGTIPDVFGSKTY 305

Query: 303 LTWLDLNHNKIFGKIPVMAVNLQ-LQLLNVSYNKLCGRIPVGGKLQSFDVYSYSNNKCLC 325
           L  LDL+HN + G+IP    + + +  L++S+NKLCGRIP G      +  S+S+N+CLC
Sbjct: 306 LVSLDLSHNSLSGRIPDSLSSAKFVGHLDISHNKLCGRIPTGFPFDHLEATSFSDNQCLC 365

BLAST of CmaCh03G000780.1 vs. TAIR 10
Match: AT3G20820.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 178.3 bits (451), Expect = 9.8e-45
Identity = 117/359 (32.59%), Postives = 178/359 (49.58%), Query Frame = 0

Query: 9   LLIFFLFFTISFAELCHPNDKKALLNIKKAFNNPY--ILTSWKPEEDCCTWYCVECDRNS 68
           +++  L   IS A  C P+D++ALL  + A + PY  I  SW  ++ C  WY + CD  +
Sbjct: 5   VVVLLLLLLISSATCCPPSDRRALLAFRSALHEPYLGIFNSWTGQDCCHNWYGISCDSLT 64

Query: 69  HRIVSLTIFADTR------------LSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIA 128
           HR+  + +  ++             ++G I   + +L  L  +       ++G I   I 
Sbjct: 65  HRVADINLRGESEDPIFERAHRTGYMTGHISASICELTRLSAITIADWKGISGEIPKCIT 124

Query: 129 KLHNLKYLDLSWNTLTGSIPNFLSSLTNLELLDLSFNRLTGSIPSSLANLQRLGTLHLDR 188
           +L  L+ LDL  N ++G IP  +  L  L +L+++ NR++GSIP SL NL  L  L L  
Sbjct: 125 RLPFLRTLDLIGNQISGGIPYDIGRLNRLAVLNVADNRISGSIPKSLTNLSSLMHLDLRN 184

Query: 189 NKLTGPIPDSFGNFKGKIPYLYLSHNQLSGKIPTSFERV-DFNVIDLSRNRLEG------ 248
           N ++G IP   G  K  +    LS N+++G+IP S   +     +DLS N+L G      
Sbjct: 185 NLISGVIPSDVGRLK-MLSRALLSGNRITGRIPESLTNIYRLADVDLSGNQLYGTIPPSL 244

Query: 249 -----------DGSVIFGGKKTTEI------VDLSRNLLEFDMSKVVLPRS-LTWLDLNH 308
                      DG+ I G    T +      ++LSRNLL+  + +   PRS  T LDL++
Sbjct: 245 GRMSVLATLNLDGNKISGEIPQTLMTSSVMNLNLSRNLLQGKIPEGFGPRSYFTVLDLSY 304

Query: 309 NKIFGKIPVMAVNLQ-LQLLNVSYNKLCGRIPVGGKLQSFDVYSYSNNKCLCGKPLGKC 328
           N + G IP        +  L++S+N LCGRIPVG      +  S+  N CLCGKPL  C
Sbjct: 305 NNLKGPIPRSISGASFIGHLDLSHNHLCGRIPVGSPFDHLEAASFMFNDCLCGKPLRAC 362

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q050911.9e-12567.79Polygalacturonase inhibitor OS=Pyrus communis OX=23211 GN=PGIP PE=1 SV=1[more]
A7PW811.2e-11665.48Polygalacturonase inhibitor OS=Vitis vinifera OX=29760 GN=pgip PE=1 SV=1[more]
Q9M5J91.6e-11160.12Polygalacturonase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PGIP1 PE=1 SV=1[more]
Q9M5J81.6e-11160.42Polygalacturonase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PGIP2 PE=2 SV=2[more]
Q9LH521.5e-8250.31Leucine-rich repeat protein FLOR 1 OS=Arabidopsis thaliana OX=3702 GN=FLR1 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1HW962.4e-192100.00polygalacturonase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111467229 PE=... [more]
A0A6J1ELI83.3e-18998.17polygalacturonase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111435518 P... [more]
Q7X9Q77.6e-16284.06Polygalacturonase-inhibiting protein OS=Cucumis melo OX=3656 GN=LOC103499550 PE=... [more]
A0A5D3BX477.6e-16284.06Polygalacturonase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
K9MZG21.7e-16184.06Polygalacturonase-inhibiting protein OS=Cucumis melo subsp. agrestis OX=217619 G... [more]
Match NameE-valueIdentityDescription
XP_022967838.15.0e-192100.00polygalacturonase inhibitor-like [Cucurbita maxima][more]
XP_022928674.16.8e-18998.17polygalacturonase inhibitor-like [Cucurbita moschata] >XP_023543570.1 polygalact... [more]
KAG6603233.13.4e-18897.56Polygalacturonase inhibitor, partial [Cucurbita argyrosperma subsp. sororia][more]
NP_001303611.11.6e-16184.06polygalacturonase inhibitor-like precursor [Cucumis melo] >AAP41199.1 polygalact... [more]
AFY10521.13.5e-16184.06polygalacturonase-inhibiting protein [Cucumis melo subsp. agrestis][more]
Match NameE-valueIdentityDescription
AT5G06860.11.1e-11260.12polygalacturonase inhibiting protein 1 [more]
AT5G06870.11.1e-11260.42polygalacturonase inhibiting protein 2 [more]
AT3G12145.11.1e-8350.31Leucine-rich repeat (LRR) family protein [more]
AT3G12610.11.1e-4833.24Leucine-rich repeat (LRR) family protein [more]
AT3G20820.19.8e-4532.59Leucine-rich repeat (LRR) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00019LEURICHRPTcoord: 140..153
score: 56.81
coord: 119..132
score: 53.88
NoneNo IPR availablePANTHERPTHR48059POLYGALACTURONASE INHIBITOR 1coord: 2..327
NoneNo IPR availablePANTHERPTHR48059:SF10POLYGALACTURONASE INHIBITOR 2coord: 2..327
NoneNo IPR availableSUPERFAMILY52058L domain-likecoord: 23..326
IPR003591Leucine-rich repeat, typical subtypeSMARTSM00369LRR_typ_2coord: 164..187
e-value: 81.0
score: 5.8
coord: 116..139
e-value: 44.0
score: 8.0
coord: 140..163
e-value: 16.0
score: 11.5
IPR001611Leucine-rich repeatPFAMPF00560LRR_1coord: 118..140
e-value: 0.0067
score: 16.8
IPR001611Leucine-rich repeatPFAMPF13855LRR_8coord: 142..202
e-value: 1.1E-7
score: 31.4
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 142..165
score: 7.32692
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 118..140
score: 7.504036
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 22..328
e-value: 1.1E-86
score: 292.9
IPR013210Leucine-rich repeat-containing N-terminal, plant-typePFAMPF08263LRRNT_2coord: 26..63
e-value: 2.4E-11
score: 43.7

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CmaCh03G000780CmaCh03G000780gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh03G000780.1:exon:472CmaCh03G000780.1:exon:472exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh03G000780.1:cdsCmaCh03G000780.1:cdsCDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CmaCh03G000780.1CmaCh03G000780.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
molecular_function GO:0005515 protein binding
molecular_function GO:0090353 polygalacturonase inhibitor activity