CmaCh02G011510.1 (mRNA) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh02G011510.1 vs. ExPASy Swiss-Prot
Match: Q00624 (L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 1.2e-97
Identity = 165/238 (69.33%), Postives = 187/238 (78.57%), Query Frame = 0

Query: 4   GLFLFALLLCLSTASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPNINS 63
           G+ L A  L L+ A+   V AEDPY    W VTYGT SPLGVPQQ ILING+FPGPNINS
Sbjct: 3   GVKLLAACLYLAAAATVVVHAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNINS 62

Query: 64  TTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIG 123
           T+NNNV++N+FN+LDEPFL+ W+GIQHRKNCWQDG PGT CPI  GTN+TYHFQ KDQIG
Sbjct: 63  TSNNNVIINVFNNLDEPFLLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQIG 122

Query: 124 SFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLRGR 183
           S+FYYP+T MHRAAGG+GGLR+NSRLLIPVPYADPEDDYTVLIGDWYTK           
Sbjct: 123 SYFYYPTTGMHRAAGGYGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTK----------- 182

Query: 184 LHRPHWRLSHTALKQLLDSGRTLARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRI 242
                   SHT LK+ LD GRT+ RPDGI+INGK+ KGDGSD PLFT+KP KTY+ RI
Sbjct: 183 --------SHTQLKKFLDGGRTIGRPDGIVINGKSGKGDGSDAPLFTLKPGKTYRVRI 221

BLAST of CmaCh02G011510.1 vs. ExPASy Swiss-Prot
Match: P29162 (L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 4.8e-86
Identity = 150/241 (62.24%), Postives = 178/241 (73.86%), Query Frame = 0

Query: 1   MGSGLFLF-ALLLCLSTASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGP 60
           MGSG   F ALLLCLS      V AEDPYL+F W VTYGTI+PLGVPQQGILING+FPGP
Sbjct: 1   MGSGKVTFVALLLCLSVG----VIAEDPYLYFNWNVTYGTIAPLGVPQQGILINGQFPGP 60

Query: 61  NINSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVK 120
            IN T+NNN+VVN+FN+LDEPFL  W+G+QHRKN WQDG PGT CPI  G NFTY FQVK
Sbjct: 61  RINCTSNNNIVVNVFNNLDEPFLFTWNGVQHRKNSWQDGTPGTMCPIMPGQNFTYRFQVK 120

Query: 121 DQIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQ 180
           DQIGS+ Y+P+TA+HRAAGG+G L ++SR LIPVP+ +P D+Y V +GDWY K       
Sbjct: 121 DQIGSYSYFPTTALHRAAGGYGALNVHSRALIPVPFDNPADEYNVFVGDWYNK------- 180

Query: 181 LRGRLHRPHWRLSHTALKQLLDSGRTLARPDGILINGKNAKGDGSDEPLFTMKPEKTYKY 240
                        H  LK++LD GRT+ RPDGI+INGK+AK   + EPLFTM+  KTY+Y
Sbjct: 181 ------------GHKTLKKILDGGRTIGRPDGIIINGKSAKVGEAKEPLFTMEAGKTYRY 218

BLAST of CmaCh02G011510.1 vs. ExPASy Swiss-Prot
Match: Q8VXX5 (Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=2 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 6.8e-64
Identity = 123/247 (49.80%), Postives = 160/247 (64.78%), Query Frame = 0

Query: 1   MGSGLFLFALLLCLSTASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPN 60
           + S L  FALL  +S A+       DP++ + ++V+Y T SPLGVPQQ I +NG+FPGP 
Sbjct: 8   LASFLLCFALLSAVSFAA-------DPFVSYDFRVSYLTASPLGVPQQVIAVNGQFPGPL 67

Query: 61  INSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKD 120
           +N+TTN NVVVN+FNHLDEP L+ W GIQ R+N WQDG+ GT CPIP   NFTY FQVKD
Sbjct: 68  LNATTNYNVVVNVFNHLDEPLLLTWPGIQMRRNSWQDGVLGTNCPIPPRWNFTYQFQVKD 127

Query: 121 QIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQL 180
           QIGSFFY PS    RA+GGFG + IN+R +IP+P+  P+ +   +IGDWYT+        
Sbjct: 128 QIGSFFYSPSLNFQRASGGFGPIVINNRDIIPIPFPQPDGELIFIIGDWYTQ-------- 187

Query: 181 RGRLHRPHWRLSHTALKQLLDSGRTLARPDGILINGK------NAKGDGSDEPLFTMKPE 240
                       H AL++ LDSG+ L  PDG+LINGK      ++  DG D   F ++P 
Sbjct: 188 -----------DHKALRRALDSGKELGMPDGVLINGKGPYKYNSSVPDGIDYLTFHVEPG 228

Query: 241 KTYKYRI 242
           KTY+ R+
Sbjct: 248 KTYRIRV 228

BLAST of CmaCh02G011510.1 vs. ExPASy Swiss-Prot
Match: Q9SU40 (Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=1 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 8.9e-64
Identity = 121/242 (50.00%), Postives = 153/242 (63.22%), Query Frame = 0

Query: 7   LFALLLCLSTASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPNINSTTN 66
           LF +LL +   ++    A DPY F+ ++V+Y T SPLGVPQQ I ING+FPGP IN TTN
Sbjct: 3   LFKILLLVFFVNISFCFAADPYSFYNFEVSYITASPLGVPQQVIAINGKFPGPTINVTTN 62

Query: 67  NNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGSFF 126
            N+VVN+ N LDE  L+HW+GIQ R+  WQDG+ GT CPIP   N+TY FQVKDQIGSFF
Sbjct: 63  ENLVVNVRNKLDEGLLLHWNGIQQRRVSWQDGVLGTNCPIPPKWNWTYEFQVKDQIGSFF 122

Query: 127 YYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLRGRLHR 186
           Y+PS    RA+GGFG   +N R +IPVP++ P+ D TV IGDWY +              
Sbjct: 123 YFPSLHFQRASGGFGSFVVNPRAIIPVPFSTPDGDITVTIGDWYIR-------------- 182

Query: 187 PHWRLSHTALKQLLDSGRTLARPDGILINGK-------NAKGDGSDEPLFTMKPEKTYKY 242
                +HTAL++ LD G+ L  PDG+LINGK           DG D    T+ P KTY+ 
Sbjct: 183 -----NHTALRKALDDGKDLGMPDGVLINGKGPYRYNDTLVADGIDFETITVHPGKTYRL 225

BLAST of CmaCh02G011510.1 vs. ExPASy Swiss-Prot
Match: Q9FHN6 (Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=2 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 4.9e-62
Identity = 117/243 (48.15%), Postives = 156/243 (64.20%), Query Frame = 0

Query: 5   LFLFALLLCLSTASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPNINST 64
           +F FAL+   S A        DPY+ + + ++Y T SPLGVPQQ I +NG+FPGP IN+T
Sbjct: 11  VFSFALIFGFSFAG-------DPYVSYDFTLSYITASPLGVPQQVIAVNGKFPGPVINAT 70

Query: 65  TNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGS 124
           TN NV VN+ NHLDEP L+ W G+Q R+N WQDG+ GT CPIP   NFTY FQ+KDQIGS
Sbjct: 71  TNYNVHVNVLNHLDEPLLLTWPGVQMRRNSWQDGVLGTNCPIPPNWNFTYDFQLKDQIGS 130

Query: 125 FFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLRGRL 184
           +FY PS    RA+GGFG L IN+R L+P+P+ +P+ +   +IGDWYT+            
Sbjct: 131 YFYSPSLNFQRASGGFGALIINNRDLVPIPFTEPDGEIIFIIGDWYTQ------------ 190

Query: 185 HRPHWRLSHTALKQLLDSGRTLARPDGILINGK------NAKGDGSDEPLFTMKPEKTYK 242
                  +HTAL+++LDSG+ L  PDG+LINGK      ++  DG +     + P KTY+
Sbjct: 191 -------NHTALRRILDSGKELGMPDGVLINGKGPFKYNSSVPDGIEHETVNVDPGKTYR 227

BLAST of CmaCh02G011510.1 vs. TAIR 10
Match: AT1G55570.1 (SKU5 similar 12 )

HSP 1 Score: 367.1 bits (941), Expect = 1.1e-101
Identity = 170/241 (70.54%), Postives = 193/241 (80.08%), Query Frame = 0

Query: 1   MGSGLFLFALLLCLSTASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPN 60
           M  G+ L A+ LC++TA++  V+AEDPY    W VTYGT SPLGVPQQ ILING+FPGPN
Sbjct: 1   MKGGVKLLAVCLCVATATVMMVQAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPN 60

Query: 61  INSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKD 120
           INST+NNNV+VN+FN+LDEPFLI W+GIQHRKNCWQDG  GT CPIP G NFTYHFQ KD
Sbjct: 61  INSTSNNNVIVNVFNNLDEPFLITWAGIQHRKNCWQDGTAGTMCPIPPGQNFTYHFQPKD 120

Query: 121 QIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQL 180
           QIGS+FYYP+TAMHRAAGGFGGLR+NSRLLIPVPYADPEDDYT+LI DWYTK        
Sbjct: 121 QIGSYFYYPTTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTILINDWYTK-------- 180

Query: 181 RGRLHRPHWRLSHTALKQLLDSGRTLARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYR 240
                      SHT LK+ LDSGRT+ RPDGILINGK+ K DGSD+PLFT+KP KTY+ R
Sbjct: 181 -----------SHTQLKKFLDSGRTIGRPDGILINGKSGKTDGSDKPLFTLKPGKTYRVR 222

Query: 241 I 242
           I
Sbjct: 241 I 222

BLAST of CmaCh02G011510.1 vs. TAIR 10
Match: AT3G13390.1 (SKU5 similar 11 )

HSP 1 Score: 366.7 bits (940), Expect = 1.4e-101
Identity = 170/238 (71.43%), Postives = 191/238 (80.25%), Query Frame = 0

Query: 4   GLFLFALLLCLSTASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPNINS 63
           G+ L A  L L+ A+   VRAEDPY    W VTYGT+SPLGVPQQ ILING+FPGPN+NS
Sbjct: 3   GVKLLAACLYLAAAATVVVRAEDPYFHHVWNVTYGTVSPLGVPQQVILINGQFPGPNVNS 62

Query: 64  TTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIG 123
           T+NNNV++N+FN+LDEPFL+ W+GIQHRKNCWQDG PGT CPI  GTN+TYHFQ KDQIG
Sbjct: 63  TSNNNVIINVFNNLDEPFLLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQIG 122

Query: 124 SFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLRGR 183
           S+FYYPSTAMHR+AGGFGGLR+NSRLLIPVPYADPEDDYTVLIGDWYTK           
Sbjct: 123 SYFYYPSTAMHRSAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTK----------- 182

Query: 184 LHRPHWRLSHTALKQLLDSGRTLARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRI 242
                   SHT LK+ LDSGRTL RPDGILINGK+ KGDGSD PLFT+KP KTY+ RI
Sbjct: 183 --------SHTQLKKFLDSGRTLGRPDGILINGKSGKGDGSDAPLFTLKPGKTYRVRI 221

BLAST of CmaCh02G011510.1 vs. TAIR 10
Match: AT1G55560.1 (SKU5 similar 14 )

HSP 1 Score: 352.1 bits (902), Expect = 3.7e-97
Identity = 162/235 (68.94%), Postives = 187/235 (79.57%), Query Frame = 0

Query: 7   LFALLLCLSTASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPNINSTTN 66
           L  +L+CL  +++  V A DPY F TW VTYGT SPLGVPQ+ ILING+FPGPN+NST+N
Sbjct: 5   LLTVLVCL-VSTVAIVNAGDPYFFHTWNVTYGTASPLGVPQKVILINGQFPGPNLNSTSN 64

Query: 67  NNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGSFF 126
           NNVV+N+FNHLDEPFL+ WSGIQHRKNCWQDG+ GT+CPIPAG NFTYHFQ KDQIGS+F
Sbjct: 65  NNVVINVFNHLDEPFLLTWSGIQHRKNCWQDGVAGTSCPIPAGQNFTYHFQPKDQIGSYF 124

Query: 127 YYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLRGRLHR 186
           YYP+T++HR AGGFGGLR+NSRLLIPVPYADPEDDYTVL+GDWYT               
Sbjct: 125 YYPTTSLHRFAGGFGGLRVNSRLLIPVPYADPEDDYTVLLGDWYT--------------- 184

Query: 187 PHWRLSHTALKQLLDSGRTLARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRI 242
                 HTALK  LDSGRTL  P+G+LINGK+ K  G +EPLFTMKP KTYKYR+
Sbjct: 185 ----AGHTALKNFLDSGRTLGLPNGVLINGKSGKVGGKNEPLFTMKPGKTYKYRL 219

BLAST of CmaCh02G011510.1 vs. TAIR 10
Match: AT3G13400.1 (SKU5 similar 13 )

HSP 1 Score: 347.4 bits (890), Expect = 9.0e-96
Identity = 163/241 (67.63%), Postives = 187/241 (77.59%), Query Frame = 0

Query: 1   MGSGLFLFALLLCLSTASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPN 60
           M  G  L  L+   ST +L  V A DPY ++TW VTYGT +PLG+PQQ ILING+FPGPN
Sbjct: 1   MQGGRLLTVLVCLASTVAL--VSAGDPYFYYTWNVTYGTAAPLGIPQQVILINGQFPGPN 60

Query: 61  INSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKD 120
           +NST+NNNVV+N+FN+LDEPFL+ WSG+QHRKN WQDG+ GT+CPIPAGTNFTYHFQ KD
Sbjct: 61  LNSTSNNNVVINVFNNLDEPFLLTWSGLQHRKNSWQDGVTGTSCPIPAGTNFTYHFQPKD 120

Query: 121 QIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQL 180
           QIGS+FYYPSTA+HR AGGFGGLR+NSRLLIPVPYADPEDD T+LI DWY K        
Sbjct: 121 QIGSYFYYPSTALHRFAGGFGGLRVNSRLLIPVPYADPEDDRTILINDWYAK-------- 180

Query: 181 RGRLHRPHWRLSHTALKQLLDSGRTLARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYR 240
                      SHTALK  LDSGRTL  PDG+LINGK+ K  G++ PLFTMKP KTYKYR
Sbjct: 181 -----------SHTALKNFLDSGRTLGSPDGVLINGKSGKLGGNNAPLFTMKPGKTYKYR 220

Query: 241 I 242
           I
Sbjct: 241 I 220

BLAST of CmaCh02G011510.1 vs. TAIR 10
Match: AT4G22010.1 (SKU5 similar 4 )

HSP 1 Score: 263.8 bits (673), Expect = 1.3e-70
Identity = 118/220 (53.64%), Postives = 158/220 (71.82%), Query Frame = 0

Query: 22  VRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPNINSTTNNNVVVNIFNHLDEPF 81
           V  ++PY FFTWK+TYG I PLGV QQGILING+FPGP+I++ TN+N+++++FN+L EPF
Sbjct: 19  VLGDNPYRFFTWKITYGDIYPLGVKQQGILINGQFPGPHIDAITNDNIIISVFNYLKEPF 78

Query: 82  LIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGSFFYYPSTAMHRAAGGFG 141
           LI W+G+Q RKN WQDG+ GTTCPIP G NFTY  QVKDQIGSF+Y+PS A H+AAG FG
Sbjct: 79  LISWNGVQQRKNSWQDGVVGTTCPIPPGKNFTYVIQVKDQIGSFYYFPSLAFHKAAGAFG 138

Query: 142 GLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLRGRLHRPHWRLSHTALKQLLD 201
            +R+ SR  IPVP++ P+ D+ +L GDWY                   + +H  L++LL+
Sbjct: 139 AIRVWSRPRIPVPFSPPDGDFWLLAGDWY-------------------KTNHYVLRRLLE 198

Query: 202 SGRTLARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRI 242
           +GR L  PDG+LING+   G+      FT++P KTY++RI
Sbjct: 199 AGRNLPNPDGVLINGRGWGGN-----TFTVQPGKTYRFRI 214