CmaCh02G004530.1 (mRNA) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh02G004530.1
Type	mRNA
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Inositol-tetrakisphosphate 1-kinase
Location	Cma_Chr02: 2352761 .. 2353741 (-)
Sequence length	981
RNA-Seq Expression	CmaCh02G004530.1
Synteny	CmaCh02G004530.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTTCCCCGCGCTTCCGAATCGGCTACGCCCTTTCCCCCAAAAAGGAGCGTTCATTCATCCTCCCTTCACTCATCGACTACGCCAAACTCCATGGCATAGACCTTGTCCGCATCGACCCTCTTCTCCCTCTTGTTCATCAGCTCCCCTTTCACTGCATCATCCACAAGCTTTACGACCCCTCCTGGGTTCAAAAATTACACGATTTCACTTCCCAATTCCCTGATGTCATCATTATTGACCCACCTAAGCTAATCTCCCGCCTTCTCAATCGTGACTCCATGCTTCAAGTCGTCAATGACGTCAAAATCCCTAAAGGGGTTGAGCGAATTGAGACACCCAGACAAGTTGTGGTGAATGATTTGGATATCATAATCAAAGATGGGTTGAATGCATTTCGCGATTTGGGGCTCAAATACCCTGTAATCGCCAAGCCCTTGGAGTCCGATGGCTCTGCAAAATCGCATCAATTGAGCTTGGTGTCGAACGATAACGGATTAAAGGGATTAAACCCACCAATTGTGCTGCAAGAATTCGTCAATCATGGCGGTGTTGTTTTCAAAGTGTATGTCGTCGGGGAGTGCGTGGTGTGCGTAACGAGGAAATCTTTGCAGGACATTTCGGCGGAGGAGGTCGAGAAATTGGAAGCGGTTTCGAGGTTTTCGCAGATATCAAATTCAGGGGCTCAAGAGGACGACGAGGGTAATGTTGAAATGCCATCTTTGGATTTTGTGATGCATGTGGCTGCTGGGTTAAGGGAAGCAACAGGGCTTAGGCTTTTTAACTTTGATATGATTAGGGATAGTAATGATCTGAATAGATATCTTGTGATTGATATTAATTACTTACCTGGGTATGCCAAAATGCCTAACTATGAGCCTTTCTTGACCAAATTTTTCTTGGATATTGTTCATAAACCGAGTGATGGGGTAGCCTTTAGTTCTTCCAATGTCAATGAAAATGTGGTAATGTTCTGTTGA

mRNA sequence

ATGTCTTCCCCGCGCTTCCGAATCGGCTACGCCCTTTCCCCCAAAAAGGAGCGTTCATTCATCCTCCCTTCACTCATCGACTACGCCAAACTCCATGGCATAGACCTTGTCCGCATCGACCCTCTTCTCCCTCTTGTTCATCAGCTCCCCTTTCACTGCATCATCCACAAGCTTTACGACCCCTCCTGGGTTCAAAAATTACACGATTTCACTTCCCAATTCCCTGATGTCATCATTATTGACCCACCTAAGCTAATCTCCCGCCTTCTCAATCGTGACTCCATGCTTCAAGTCGTCAATGACGTCAAAATCCCTAAAGGGGTTGAGCGAATTGAGACACCCAGACAAGTTGTGGTGAATGATTTGGATATCATAATCAAAGATGGGTTGAATGCATTTCGCGATTTGGGGCTCAAATACCCTGTAATCGCCAAGCCCTTGGAGTCCGATGGCTCTGCAAAATCGCATCAATTGAGCTTGGTGTCGAACGATAACGGATTAAAGGGATTAAACCCACCAATTGTGCTGCAAGAATTCGTCAATCATGGCGGTGTTGTTTTCAAAGTGTATGTCGTCGGGGAGTGCGTGGTGTGCGTAACGAGGAAATCTTTGCAGGACATTTCGGCGGAGGAGGTCGAGAAATTGGAAGCGGTTTCGAGGTTTTCGCAGATATCAAATTCAGGGGCTCAAGAGGACGACGAGGGTAATGTTGAAATGCCATCTTTGGATTTTGTGATGCATGTGGCTGCTGGGTTAAGGGAAGCAACAGGGCTTAGGCTTTTTAACTTTGATATGATTAGGGATAGTAATGATCTGAATAGATATCTTGTGATTGATATTAATTACTTACCTGGGTATGCCAAAATGCCTAACTATGAGCCTTTCTTGACCAAATTTTTCTTGGATATTGTTCATAAACCGAGTGATGGGGTAGCCTTTAGTTCTTCCAATGTCAATGAAAATGTGGTAATGTTCTGTTGA

Coding sequence (CDS)

ATGTCTTCCCCGCGCTTCCGAATCGGCTACGCCCTTTCCCCCAAAAAGGAGCGTTCATTCATCCTCCCTTCACTCATCGACTACGCCAAACTCCATGGCATAGACCTTGTCCGCATCGACCCTCTTCTCCCTCTTGTTCATCAGCTCCCCTTTCACTGCATCATCCACAAGCTTTACGACCCCTCCTGGGTTCAAAAATTACACGATTTCACTTCCCAATTCCCTGATGTCATCATTATTGACCCACCTAAGCTAATCTCCCGCCTTCTCAATCGTGACTCCATGCTTCAAGTCGTCAATGACGTCAAAATCCCTAAAGGGGTTGAGCGAATTGAGACACCCAGACAAGTTGTGGTGAATGATTTGGATATCATAATCAAAGATGGGTTGAATGCATTTCGCGATTTGGGGCTCAAATACCCTGTAATCGCCAAGCCCTTGGAGTCCGATGGCTCTGCAAAATCGCATCAATTGAGCTTGGTGTCGAACGATAACGGATTAAAGGGATTAAACCCACCAATTGTGCTGCAAGAATTCGTCAATCATGGCGGTGTTGTTTTCAAAGTGTATGTCGTCGGGGAGTGCGTGGTGTGCGTAACGAGGAAATCTTTGCAGGACATTTCGGCGGAGGAGGTCGAGAAATTGGAAGCGGTTTCGAGGTTTTCGCAGATATCAAATTCAGGGGCTCAAGAGGACGACGAGGGTAATGTTGAAATGCCATCTTTGGATTTTGTGATGCATGTGGCTGCTGGGTTAAGGGAAGCAACAGGGCTTAGGCTTTTTAACTTTGATATGATTAGGGATAGTAATGATCTGAATAGATATCTTGTGATTGATATTAATTACTTACCTGGGTATGCCAAAATGCCTAACTATGAGCCTTTCTTGACCAAATTTTTCTTGGATATTGTTCATAAACCGAGTGATGGGGTAGCCTTTAGTTCTTCCAATGTCAATGAAAATGTGGTAATGTTCTGTTGA

Protein sequence

MSSPRFRIGYALSPKKERSFILPSLIDYAKLHGIDLVRIDPLLPLVHQLPFHCIIHKLYDPSWVQKLHDFTSQFPDVIIIDPPKLISRLLNRDSMLQVVNDVKIPKGVERIETPRQVVVNDLDIIIKDGLNAFRDLGLKYPVIAKPLESDGSAKSHQLSLVSNDNGLKGLNPPIVLQEFVNHGGVVFKVYVVGECVVCVTRKSLQDISAEEVEKLEAVSRFSQISNSGAQEDDEGNVEMPSLDFVMHVAAGLREATGLRLFNFDMIRDSNDLNRYLVIDINYLPGYAKMPNYEPFLTKFFLDIVHKPSDGVAFSSSNVNENVVMFC

Homology

[Close]

BLAST of CmaCh02G004530.1 vs. ExPASy Swiss-Prot
Match: Q9SBA5 (Inositol-tetrakisphosphate 1-kinase 1 OS=Arabidopsis thaliana OX=3702 GN=ITPK1 PE=1 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 1.4e-83
Identity = 167/313 (53.35%), Postives = 213/313 (68.05%), Query Frame = 0


Query: 5   RFRIGYALSPKKERSFILPSLIDYAKLHGIDLVRIDPLLPLVHQLPFHCIIHKLYDPSWV 64
           R+ +GYAL+ KK+ SFI PSLI++++  GIDLV++DP   L+ Q    CIIHKLYD  W 
Sbjct: 8   RYLVGYALAAKKQHSFIQPSLIEHSRQRGIDLVKLDPTKSLLEQGKLDCIIHKLYDVYWK 67

Query: 65  QKLHDFTSQFPDVIIIDPPKLISRLLNRDSMLQVVNDVKIP-KGVERIETPRQVVVNDLD 124
           + LH+F  + P V +ID P+ I RL NR SML+V+  ++ P    ER   P QVVV D  
Sbjct: 68  ENLHEFREKCPGVPVIDLPEAIERLHNRVSMLEVITQLRFPVSDSERFGVPEQVVVMDSS 127

Query: 125 IIIKDGLNAFRDLG-LKYPVIAKPLESDGSAKSHQLSLVSNDNGLKGLNPPIVLQEFVNH 184
           ++   G      LG LK+PVIAKPL++DGSAKSH++ L+ +  G+K L  PIVLQEFVNH
Sbjct: 128 VLSGGGA-----LGELKFPVIAKPLDADGSAKSHKMFLIYDQEGMKILKAPIVLQEFVNH 187

Query: 185 GGVVFKVYVVGECVVCVTRKSLQDISAEEVEKLEAVSRFSQISNSGAQEDDE-------- 244
           GGV+FKVYVVG+ V CV R+SL DIS E++   +    FSQISN  AQED          
Sbjct: 188 GGVIFKVYVVGDHVKCVKRRSLPDISEEKIGTSKGSLPFSQISNLTAQEDKNIEYGEDRS 247

Query: 245 -GNVEMPSLDFVMHVAAGLREATGLRLFNFDMIRDSNDLNRYLVIDINYLPGYAKMPNYE 304
              VEMP L F+  +A  +RE+ GL LFNFD+IRD+ D NRYL+IDINY PGYAKMP+YE
Sbjct: 248 LEKVEMPPLSFLTDLAKAMRESMGLNLFNFDVIRDAKDANRYLIIDINYFPGYAKMPSYE 307

Query: 305 PFLTKFFLDIVHK 307
           P LT+FF D+V K
Sbjct: 308 PVLTEFFWDMVTK 315

[Close]

BLAST of CmaCh02G004530.1 vs. ExPASy Swiss-Prot
Match: A0JJZ6 (Inositol-tetrakisphosphate 1-kinase 5 OS=Oryza sativa subsp. indica OX=39946 GN=ITPK5 PE=3 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 1.3e-78
Identity = 166/330 (50.30%), Postives = 214/330 (64.85%), Query Frame = 0


Query: 5   RFRIGYALSPKKERSFILPSLIDYAKLHGIDLVRIDPLLPLVHQLPFHCIIHKLYDPSWV 64
           R+ IGYAL+PKK++SFI PSL+  A   G+DLV +DP  PL  Q PFH +IHKLY   W 
Sbjct: 15  RYLIGYALAPKKQQSFIQPSLVSRAAGRGMDLVPVDPSRPLPEQGPFHLLIHKLYGEEWR 74

Query: 65  QKLHDFTSQFPDVIIIDPPKLISRLLNRDSMLQVVNDVKIPKGVERIET---PRQVVVND 124
            +L  F++  P V ++DPP  I RL NR SMLQVV+++ +P       T   P QVVV D
Sbjct: 75  GQLDAFSAAHPAVPVVDPPHAIDRLHNRISMLQVVSELDVPLHAHHHHTFGIPSQVVVYD 134

Query: 125 LDIIIKDGLNAFRDLGLKYPVIAKPLESDGSAKSHQLSLVSNDNGLKGLNPPIVLQEFVN 184
              +   GL A     L++P+IAKPL +DG+AKSH++SLV +  GL+ L PP+VLQEFVN
Sbjct: 135 AAALSDSGLLA----ALRFPLIAKPLVADGTAKSHKMSLVYHREGLRKLRPPLVLQEFVN 194

Query: 185 HGGVVFKVYVVGECVVCVTRKSLQDISAEEVE--KLEAVSRFSQISN-----SGAQEDDE 244
           HGGV+FKVYVVG  V CV R+SL D+S++ ++    E    FSQ+SN     +  +  D+
Sbjct: 195 HGGVIFKVYVVGAHVTCVKRRSLPDVSSDVLQDASAEGSLSFSQVSNLPNERTAQEYYDD 254

Query: 245 GNVE---MPSLDFVMHVAAGLREATGLRLFNFDMIRDSNDLNRYLVIDINYLPGYAKMPN 304
             +E   MP   F+  +AA LR A GL LFNFDMIRD+   +RYLVIDINY PGYAKMP 
Sbjct: 255 MRLEDAIMPPTAFINDIAAALRRALGLHLFNFDMIRDARAGDRYLVIDINYFPGYAKMPG 314

Query: 305 YEPFLTKFFLDIVHKPSDGVAFSSSNVNEN 322
           YE  LT FF ++VHK  D     + N+N N
Sbjct: 315 YETVLTDFFWEMVHKDDD-----TPNLNPN 335

[Close]

BLAST of CmaCh02G004530.1 vs. ExPASy Swiss-Prot
Match: Q7XBW0 (Inositol-tetrakisphosphate 1-kinase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=ITPK5 PE=2 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 1.3e-78
Identity = 166/330 (50.30%), Postives = 214/330 (64.85%), Query Frame = 0


Query: 5   RFRIGYALSPKKERSFILPSLIDYAKLHGIDLVRIDPLLPLVHQLPFHCIIHKLYDPSWV 64
           R+ IGYAL+PKK++SFI PSL+  A   G+DLV +DP  PL  Q PFH +IHKLY   W 
Sbjct: 15  RYLIGYALAPKKQQSFIQPSLVSRAAGRGMDLVPVDPSRPLPEQGPFHLLIHKLYGEEWR 74

Query: 65  QKLHDFTSQFPDVIIIDPPKLISRLLNRDSMLQVVNDVKIPKGVERIET---PRQVVVND 124
            +L  F++  P V ++DPP  I RL NR SMLQVV+++ +P       T   P QVVV D
Sbjct: 75  GQLDAFSAAHPAVPVVDPPHAIDRLHNRISMLQVVSELDVPLHAHHHHTFGIPSQVVVYD 134

Query: 125 LDIIIKDGLNAFRDLGLKYPVIAKPLESDGSAKSHQLSLVSNDNGLKGLNPPIVLQEFVN 184
              +   GL A     L++P+IAKPL +DG+AKSH++SLV +  GL+ L PP+VLQEFVN
Sbjct: 135 AAALSDSGLLA----ALRFPLIAKPLVADGTAKSHKMSLVYHREGLRKLRPPLVLQEFVN 194

Query: 185 HGGVVFKVYVVGECVVCVTRKSLQDISAEEVE--KLEAVSRFSQISN-----SGAQEDDE 244
           HGGV+FKVYVVG  V CV R+SL D+S++ ++    E    FSQ+SN     +  +  D+
Sbjct: 195 HGGVIFKVYVVGAHVTCVKRRSLPDVSSDVLQDASAEGSLSFSQVSNLPNERTAQEYYDD 254

Query: 245 GNVE---MPSLDFVMHVAAGLREATGLRLFNFDMIRDSNDLNRYLVIDINYLPGYAKMPN 304
             +E   MP   F+  +AA LR A GL LFNFDMIRD+   +RYLVIDINY PGYAKMP 
Sbjct: 255 MRLEDAIMPPTAFINDIAAALRRALGLHLFNFDMIRDARAGDRYLVIDINYFPGYAKMPG 314

Query: 305 YEPFLTKFFLDIVHKPSDGVAFSSSNVNEN 322
           YE  LT FF ++VHK  D     + N+N N
Sbjct: 315 YETVLTDFFWEMVHKDDD-----TPNLNPN 335

[Close]

BLAST of CmaCh02G004530.1 vs. ExPASy Swiss-Prot
Match: Q84Y01 (Inositol-tetrakisphosphate 1-kinase 1 OS=Zea mays OX=4577 GN=ITPK1 PE=2 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 1.2e-76
Identity = 163/329 (49.54%), Postives = 204/329 (62.01%), Query Frame = 0


Query: 4   PRFRIGYALSPKKERSFILPSLIDYAKLHGIDLVRIDPLLPLVHQLPFHCIIHKLYDPSW 63
           PR+ IGYAL+PKK++SFI PSL+  A   G+DLV +D   PL  Q PFH +IHKLY   W
Sbjct: 17  PRYVIGYALAPKKQQSFIQPSLVAQAASRGMDLVPVDASQPLAEQGPFHLLIHKLYGDDW 76

Query: 64  VQKLHDFTSQFPDVIIIDPPKLISRLLNRDSMLQVVNDVKIPKGVE-RIETPRQVVVNDL 123
             +L  F ++ P V I+DPP  I RL NR SMLQVV+++      +     P QVVV D 
Sbjct: 77  RAQLVAFAARHPAVPIVDPPHAIDRLHNRISMLQVVSELDHAADQDSTFGIPSQVVVYDA 136

Query: 124 DIIIKDGLNAFRDLGLKYPVIAKPLESDGSAKSHQLSLVSNDNGLKGLNPPIVLQEFVNH 183
             +   GL A     L++P+IAKPL +DG+AKSH++SLV +  GL  L PP+VLQEFVNH
Sbjct: 137 AALADFGLLA----ALRFPLIAKPLVADGTAKSHKMSLVYHREGLGKLRPPLVLQEFVNH 196

Query: 184 GGVVFKVYVVGECVVCVTRKSLQDISAEEVEKLEAVSRFSQISNSGAQEDDE-------- 243
           GGV+FKVYVVG  V CV R+SL D+S E+    +    FSQ+SN   +   E        
Sbjct: 197 GGVIFKVYVVGGHVTCVKRRSLPDVSPEDDASAQGSVSFSQVSNLPTERTAEEYYGEKSL 256

Query: 244 GNVEMPSLDFVMHVAAGLREATGLRLFNFDMIRDSNDLNRYLVIDINYLPGYAKMPNYEP 303
            +  +P   F+  +A GLR A GL+LFNFDMIRD    +RYLVIDINY PGYAKMP YE 
Sbjct: 257 EDAVVPPAAFINQIAGGLRRALGLQLFNFDMIRDVRAGDRYLVIDINYFPGYAKMPGYET 316

Query: 304 FLTKFFLDIVHKPSDGVAFSSSNVNENVV 324
            LT FF ++VHK   G        N  VV
Sbjct: 317 VLTDFFWEMVHKDGVGNQQEEKGANHVVV 341

[Close]

BLAST of CmaCh02G004530.1 vs. ExPASy Swiss-Prot
Match: A2Z4C5 (Inositol-tetrakisphosphate 1-kinase 1 OS=Oryza sativa subsp. indica OX=39946 GN=ITPK1 PE=3 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 1.4e-64
Identity = 137/311 (44.05%), Postives = 183/311 (58.84%), Query Frame = 0


Query: 1   MSSPRFRIGYALSPKKERSFILPSLIDYAKLHGIDLVRIDPLLPLVHQLPFHCIIHKLYD 60
           ++  R  +GYAL+ KK +SF+ P L+  A+   I  V ID   PL  Q PF  I+HKL D
Sbjct: 39  VAGQRLVVGYALTKKKVKSFLQPKLLSLARKKSIHFVSIDETRPLSEQGPFDIILHKLTD 98

Query: 61  PSWVQKLHDFTSQFPDVIIIDPPKLISRLLNRDSMLQVVNDVKIPKGVERIETPRQVVVN 120
             W Q L D+  + P+V ++DPP  I  L NR SMLQ V D+ +      + TPRQ+V  
Sbjct: 99  KEWQQVLEDYREEHPEVTVLDPPNAIQHLHNRQSMLQEVADLNLSNAYGEVCTPRQLV-- 158

Query: 121 DLDIIIKDGL---NAFRDLGLKYPVIAKPLESDGSAKSHQLSLVSNDNGLKGLNPPIVLQ 180
               I+KD L   +A    GL  P++AKPL  DG++KSH+LSL   +  L  L+PP+VLQ
Sbjct: 159 ----IMKDPLSIPSAVAKAGLTLPLVAKPLVVDGTSKSHELSLAYVETSLSMLDPPLVLQ 218

Query: 181 EFVNHGGVVFKVYVVGECVVCVTRKSLQDISAEEVEKLEAVSRFSQIS----NSGAQEDD 240
           EFVNHGG++FKVYVVGE +  V R SL D++  ++E  + + RF ++S     +   E D
Sbjct: 219 EFVNHGGILFKVYVVGETIRVVRRFSLPDVNIYDLENNDGIFRFPRVSCATNTAEDAEVD 278

Query: 241 EGNVEMPSLDFVMHVAAGLREATGLRLFNFDMIRDSNDLNRYLVIDINYLPGYAKMPNYE 300
               E+P    +  +   LR   GLRLFNFDMIR+    +RY VIDINY PGY KMP YE
Sbjct: 279 PSIAELPPKPLLEKLGRELRRRLGLRLFNFDMIREHGRKDRYYVIDINYFPGYGKMPGYE 338

Query: 301 PFLTKFFLDIV 305
                F L +V
Sbjct: 339 HIFIDFLLSLV 343

[Close]

BLAST of CmaCh02G004530.1 vs. TAIR 10
Match: AT5G16760.1 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 311.2 bits (796), Expect = 9.7e-85
Identity = 167/313 (53.35%), Postives = 213/313 (68.05%), Query Frame = 0


Query: 5   RFRIGYALSPKKERSFILPSLIDYAKLHGIDLVRIDPLLPLVHQLPFHCIIHKLYDPSWV 64
           R+ +GYAL+ KK+ SFI PSLI++++  GIDLV++DP   L+ Q    CIIHKLYD  W 
Sbjct: 8   RYLVGYALAAKKQHSFIQPSLIEHSRQRGIDLVKLDPTKSLLEQGKLDCIIHKLYDVYWK 67

Query: 65  QKLHDFTSQFPDVIIIDPPKLISRLLNRDSMLQVVNDVKIP-KGVERIETPRQVVVNDLD 124
           + LH+F  + P V +ID P+ I RL NR SML+V+  ++ P    ER   P QVVV D  
Sbjct: 68  ENLHEFREKCPGVPVIDLPEAIERLHNRVSMLEVITQLRFPVSDSERFGVPEQVVVMDSS 127

Query: 125 IIIKDGLNAFRDLG-LKYPVIAKPLESDGSAKSHQLSLVSNDNGLKGLNPPIVLQEFVNH 184
           ++   G      LG LK+PVIAKPL++DGSAKSH++ L+ +  G+K L  PIVLQEFVNH
Sbjct: 128 VLSGGGA-----LGELKFPVIAKPLDADGSAKSHKMFLIYDQEGMKILKAPIVLQEFVNH 187

Query: 185 GGVVFKVYVVGECVVCVTRKSLQDISAEEVEKLEAVSRFSQISNSGAQEDDE-------- 244
           GGV+FKVYVVG+ V CV R+SL DIS E++   +    FSQISN  AQED          
Sbjct: 188 GGVIFKVYVVGDHVKCVKRRSLPDISEEKIGTSKGSLPFSQISNLTAQEDKNIEYGEDRS 247

Query: 245 -GNVEMPSLDFVMHVAAGLREATGLRLFNFDMIRDSNDLNRYLVIDINYLPGYAKMPNYE 304
              VEMP L F+  +A  +RE+ GL LFNFD+IRD+ D NRYL+IDINY PGYAKMP+YE
Sbjct: 248 LEKVEMPPLSFLTDLAKAMRESMGLNLFNFDVIRDAKDANRYLIIDINYFPGYAKMPSYE 307

Query: 305 PFLTKFFLDIVHK 307
           P LT+FF D+V K
Sbjct: 308 PVLTEFFWDMVTK 315

[Close]

BLAST of CmaCh02G004530.1 vs. TAIR 10
Match: AT4G08170.2 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 245.4 bits (625), Expect = 6.5e-65
Identity = 135/301 (44.85%), Postives = 179/301 (59.47%), Query Frame = 0


Query: 8   IGYALSPKKERSFILPSLIDYAKLHGIDLVRIDPLLPLVHQLPFHCIIHKLYDPSWVQKL 67
           +GYAL+ KK +SF+ P L   A+  GI  V ID   PL  Q PF  ++HK     W + L
Sbjct: 43  VGYALTSKKIKSFLQPKLEGLARNKGILFVAIDQNKPLSEQGPFDIVLHKQIGKEWRRIL 102

Query: 68  HDFTSQFPDVIIIDPPKLISRLLNRDSMLQVVNDVKIPKGVERIETPRQVVVNDLDIIIK 127
            +F    PDV ++DPP  I  L NR SMLQ V D+ +     R+  P+Q+V+      I 
Sbjct: 103 EEFRLAHPDVTVLDPPDAILHLRNRQSMLQCVADMNLSDSNGRVGVPKQLVIKKDASSIP 162

Query: 128 DGLNAFRDLGLKYPVIAKPLESDGSAKSHQLSLVSNDNGLKGLNPPIVLQEFVNHGGVVF 187
           + +N   + GL+ P++AKPL +DGSAKSH+LSL  + + L  L PP+VLQEFVNHGGV+F
Sbjct: 163 EAVN---NAGLRLPLVAKPLVADGSAKSHELSLAYDQHSLLKLEPPLVLQEFVNHGGVLF 222

Query: 188 KVYVVGECVVCVTRKSLQDISAEEVEKLEAVSRFSQISNSGAQED----DEGNVEMPSLD 247
           KVY+VGE +  V R SL D+S  E+ K   V RF ++S + A  D    D    E+P   
Sbjct: 223 KVYIVGEAIRVVRRFSLPDVSRRELPKSAGVFRFPRVSCAAASADDADLDPSIAELPPRP 282

Query: 248 FVMHVAAGLREATGLRLFNFDMIRDSNDLNRYLVIDINYLPGYAKMPNYEPFLTKFFLDI 305
            +  +A  LR   GLRLFN D+IR+    +R+ VIDINY PGY KMP YE   T F L +
Sbjct: 283 LLERLAKELRRGLGLRLFNLDIIREHGTRDRFYVIDINYFPGYGKMPEYEHVFTDFLLSV 340

[Close]

BLAST of CmaCh02G004530.1 vs. TAIR 10
Match: AT4G33770.1 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 233.8 bits (595), Expect = 2.0e-61
Identity = 129/303 (42.57%), Postives = 184/303 (60.73%), Query Frame = 0


Query: 8   IGYALSPKKERSFILPSLIDYAKLHGIDLVRIDPLLPLVHQLPFHCIIHKLYDPSWVQKL 67
           +GYAL+ KK++SF+ P L   A+  GI  V ID   PL  Q PF  ++HKL    W + +
Sbjct: 83  VGYALTSKKKKSFLQPKLELLARRKGIFFVAIDLNRPLSEQGPFDVVLHKLLGKEWEEVI 142

Query: 68  HDFTSQFPDVIIIDPPKLISRLLNRDSMLQVVNDVKIPKGVERIETPRQVVVNDLDIIIK 127
            D+  + P+V ++DPP  I R+ NR SMLQ + D+K+      +  P+Q+VV      +K
Sbjct: 143 EDYQQKHPEVTVLDPPGSIQRIYNRQSMLQGMADLKLSDCSGSLFVPKQMVV------LK 202

Query: 128 D---GLNAFRDLGLKYPVIAKPLESDGSAKSHQLSLVSNDNGLKGLNPPIVLQEFVNHGG 187
           D     +A  + GLK+P++AKPL  DG+AKSHQL L  +   L  L+PP+VLQEFVNHGG
Sbjct: 203 DSAASADAVVEAGLKFPLVAKPLWIDGTAKSHQLYLAYDRRSLAELDPPLVLQEFVNHGG 262

Query: 188 VVFKVYVVGECVVCVTRKSLQDISAEEVEKLEAVSRFSQISNSGAQED----DEGNVEMP 247
           V+FKV+VVG+ +  + R SL ++S  E  K++ V +F ++S++ A  D    D    E+P
Sbjct: 263 VMFKVFVVGDVIKVMRRFSLPNVSNCEKAKVDGVFQFPRVSSAAASADNADLDPRVAELP 322

Query: 248 SLDFVMHVAAGLREATGLRLFNFDMIRDSNDLNRYLVIDINYLPGYAKMPNYEPFLTKFF 304
              F+  +   LR   GLRLFN DMIR+    N + VIDINY PGY K+P+YE     FF
Sbjct: 323 PKPFLEALVKELRSLLGLRLFNIDMIREHGSKNVFYVIDINYFPGYGKLPDYEQVFVDFF 379

[Close]

BLAST of CmaCh02G004530.1 vs. TAIR 10
Match: AT4G33770.2 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 213.0 bits (541), Expect = 3.6e-55
Identity = 118/278 (42.45%), Postives = 167/278 (60.07%), Query Frame = 0


Query: 33  GIDLVRIDPLLPLVHQLPFHCIIHKLYDPSWVQKLHDFTSQFPDVIIIDPPKLISRLLNR 92
           GI  V ID   PL  Q PF  ++HKL    W + + D+  + P+V ++DPP  I R+ NR
Sbjct: 15  GIFFVAIDLNRPLSEQGPFDVVLHKLLGKEWEEVIEDYQQKHPEVTVLDPPGSIQRIYNR 74

Query: 93  DSMLQVVNDVKIPKGVERIETPRQVVVNDLDIIIKD---GLNAFRDLGLKYPVIAKPLES 152
            SMLQ + D+K+      +  P+Q+VV      +KD     +A  + GLK+P++AKPL  
Sbjct: 75  QSMLQGMADLKLSDCSGSLFVPKQMVV------LKDSAASADAVVEAGLKFPLVAKPLWI 134

Query: 153 DGSAKSHQLSLVSNDNGLKGLNPPIVLQEFVNHGGVVFKVYVVGECVVCVTRKSLQDISA 212
           DG+AKSHQL L  +   L  L+PP+VLQEFVNHGGV+FKV+VVG+ +  + R SL ++S 
Sbjct: 135 DGTAKSHQLYLAYDRRSLAELDPPLVLQEFVNHGGVMFKVFVVGDVIKVMRRFSLPNVSN 194

Query: 213 EEVEKLEAVSRFSQISNSGAQED----DEGNVEMPSLDFVMHVAAGLREATGLRLFNFDM 272
            E  K++ V +F ++S++ A  D    D    E+P   F+  +   LR   GLRLFN DM
Sbjct: 195 CEKAKVDGVFQFPRVSSAAASADNADLDPRVAELPPKPFLEALVKELRSLLGLRLFNIDM 254

Query: 273 IRDSNDLNRYLVIDINYLPGYAKMPNYEPFLTKFFLDI 304
           IR+    N + VIDINY PGY K+P+YE     FF ++
Sbjct: 255 IREHGSKNVFYVIDINYFPGYGKLPDYEQVFVDFFQNL 286

[Close]

BLAST of CmaCh02G004530.1 vs. TAIR 10
Match: AT4G08170.1 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 207.6 bits (527), Expect = 1.5e-53
Identity = 112/246 (45.53%), Postives = 149/246 (60.57%), Query Frame = 0


Query: 63  WVQKLHDFTSQFPDVIIIDPPKLISRLLNRDSMLQVVNDVKIPKGVERIETPRQVVVNDL 122
           W + L +F    PDV ++DPP  I  L NR SMLQ V D+ +     R+  P+Q+V+   
Sbjct: 10  WRRILEEFRLAHPDVTVLDPPDAILHLRNRQSMLQCVADMNLSDSNGRVGVPKQLVIKKD 69

Query: 123 DIIIKDGLNAFRDLGLKYPVIAKPLESDGSAKSHQLSLVSNDNGLKGLNPPIVLQEFVNH 182
              I + +N   + GL+ P++AKPL +DGSAKSH+LSL  + + L  L PP+VLQEFVNH
Sbjct: 70  ASSIPEAVN---NAGLRLPLVAKPLVADGSAKSHELSLAYDQHSLLKLEPPLVLQEFVNH 129

Query: 183 GGVVFKVYVVGECVVCVTRKSLQDISAEEVEKLEAVSRFSQISNSGAQED----DEGNVE 242
           GGV+FKVY+VGE +  V R SL D+S  E+ K   V RF ++S + A  D    D    E
Sbjct: 130 GGVLFKVYIVGEAIRVVRRFSLPDVSRRELPKSAGVFRFPRVSCAAASADDADLDPSIAE 189

Query: 243 MPSLDFVMHVAAGLREATGLRLFNFDMIRDSNDLNRYLVIDINYLPGYAKMPNYEPFLTK 302
           +P    +  +A  LR   GLRLFN D+IR+    +R+ VIDINY PGY KMP YE   T 
Sbjct: 190 LPPRPLLERLAKELRRGLGLRLFNLDIIREHGTRDRFYVIDINYFPGYGKMPEYEHVFTD 249

Query: 303 FFLDIV 305
           F L +V
Sbjct: 250 FLLSVV 252

The following BLAST results are available for this feature:

BLAST of CmaCh02G004530.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SBA5	1.4e-83	53.35	Inositol-tetrakisphosphate 1-kinase 1 OS=Arabidopsis thaliana OX=3702 GN=ITPK1 P...	[more]
A0JJZ6	1.3e-78	50.30	Inositol-tetrakisphosphate 1-kinase 5 OS=Oryza sativa subsp. indica OX=39946 GN=...	[more]
Q7XBW0	1.3e-78	50.30	Inositol-tetrakisphosphate 1-kinase 5 OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
Q84Y01	1.2e-76	49.54	Inositol-tetrakisphosphate 1-kinase 1 OS=Zea mays OX=4577 GN=ITPK1 PE=2 SV=1	[more]
A2Z4C5	1.4e-64	44.05	Inositol-tetrakisphosphate 1-kinase 1 OS=Oryza sativa subsp. indica OX=39946 GN=...	[more]

BLAST of CmaCh02G004530.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G16760.1	9.7e-85	53.35	Inositol 1,3,4-trisphosphate 5/6-kinase family protein	[more]
AT4G08170.2	6.5e-65	44.85	Inositol 1,3,4-trisphosphate 5/6-kinase family protein	[more]
AT4G33770.1	2.0e-61	42.57	Inositol 1,3,4-trisphosphate 5/6-kinase family protein	[more]
AT4G33770.2	3.6e-55	42.45	Inositol 1,3,4-trisphosphate 5/6-kinase family protein	[more]
AT4G08170.1	1.5e-53	45.53	Inositol 1,3,4-trisphosphate 5/6-kinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR041429	Inositol-tetrakisphosphate 1-kinase, N-terminal	PFAM	PF17927	Ins134_P3_kin_N	coord: 7..86 e-value: 4.3E-26 score: 91.1
IPR040464	Inositol 1,3,4-trisphosphate 5/6-kinase, ATP-grasp domain	PFAM	PF05770	Ins134_P3_kin	coord: 109..302 e-value: 4.7E-71 score: 238.5
IPR008656	Inositol-tetrakisphosphate 1-kinase	PIRSF	PIRSF038186	ITPK	coord: 1..315 e-value: 5.1E-107 score: 355.6
IPR008656	Inositol-tetrakisphosphate 1-kinase	PANTHER	PTHR14217	INOSITOL-TETRAKISPHOSPHATE 1-KINASE	coord: 2..307
None	No IPR available	GENE3D	3.30.470.20		coord: 82..285 e-value: 6.8E-56 score: 191.3
None	No IPR available	PANTHER	PTHR14217:SF24	INOSITOL-TETRAKISPHOSPHATE 1-KINASE 1	coord: 2..307
None	No IPR available	SUPERFAMILY	56059	Glutathione synthetase ATP-binding domain-like	coord: 82..290
IPR011761	ATP-grasp fold	PROSITE	PS50975	ATP_GRASP	coord: 103..312 score: 10.940201

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmaCh02G004530	CmaCh02G004530	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh02G004530.1:exon:2153	CmaCh02G004530.1:exon:2153	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh02G004530.1:cds	CmaCh02G004530.1:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmaCh02G004530.1	CmaCh02G004530.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032957	inositol trisphosphate metabolic process
biological_process	GO:0052746	inositol phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0052726	inositol-1,3,4-trisphosphate 5-kinase activity
molecular_function	GO:0052725	inositol-1,3,4-trisphosphate 6-kinase activity
molecular_function	GO:0047325	inositol tetrakisphosphate 1-kinase activity
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0046872	metal ion binding