CmUC07G127360.1 (mRNA) Watermelon (USVL531) v1

Overview


Name	CmUC07G127360.1
Type	mRNA
Organism	Citrullus mucosospermus (Watermelon (USVL531) v1)
Description	Trypsin inhibitor
Location	CmU531Chr07: 774505 .. 774843 (+)
Sequence length	225
RNA-Seq Expression	CmUC07G127360.1
Synteny	CmUC07G127360.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGAAGAAAAAATTGTTCTTCCTTCAGGTTAGTAAGATTTCTCCTTCCCACTTGTGTGTTTTCGAATGATTTTTTGTCAATTTATTTTTCCTTAATATTTTGAATAACTATTTACTTTTTCACCATTTAATTTACCTATAGGGAAATCTTCGTGGCCCGAACTCGTATTCATCGAATCTTCCACTGCAGTATGTCTTATAGAGAGAGATAATCCTAATGTGAAGGTAGTTGTGCTATTAGCTGGATCTCCAGTCACTAAAGATTTAAGGCTAGATCGAGTTCGAGTTTTTGTTAACATAAATAGTGTGGTGGTTAGCGTTCCAACGGTTGGTTGA

mRNA sequence

ATGGCTGAAGAAAAAATTGTTCTTCCTTCAGGGAAATCTTCGTGGCCCGAACTCGTATTCATCGAATCTTCCACTGCAGTATGTCTTATAGAGAGAGATAATCCTAATGTGAAGGTAGTTGTGCTATTAGCTGGATCTCCAGTCACTAAAGATTTAAGGCTAGATCGAGTTCGAGTTTTTGTTAACATAAATAGTGTGGTGGTTAGCGTTCCAACGGTTGGTTGA

Coding sequence (CDS)

ATGGCTGAAGAAAAAATTGTTCTTCCTTCAGGGAAATCTTCGTGGCCCGAACTCGTATTCATCGAATCTTCCACTGCAGTATGTCTTATAGAGAGAGATAATCCTAATGTGAAGGTAGTTGTGCTATTAGCTGGATCTCCAGTCACTAAAGATTTAAGGCTAGATCGAGTTCGAGTTTTTGTTAACATAAATAGTGTGGTGGTTAGCGTTCCAACGGTTGGTTGA

Protein sequence

MAEEKIVLPSGKSSWPELVFIESSTAVCLIERDNPNVKVVVLLAGSPVTKDLRLDRVRVFVNINSVVVSVPTVG

Homology

[Close]

BLAST of CmUC07G127360.1 vs. NCBI nr
Match: XP_011654473.1 (inhibitor of trypsin and hageman factor [Cucumis sativus] >KGN49621.1 hypothetical protein Csa_018430 [Cucumis sativus])

HSP 1 Score: 102.8 bits (255), Expect = 1.3e-18
Identity = 55/75 (73.33%), Postives = 62/75 (82.67%), Query Frame = 0


Query: 1  MAEEKIVLP-SGKSSWPELVFIESSTAVCLIERDNPNVKVVVLLAGSPVTKDLRLDRVRV 60
          MAEEKIV P SGKSSWPELVF++SS AV  IERD P+VK VVLLAGSPVT+DLR +RVR+
Sbjct: 1  MAEEKIVFPSSGKSSWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPVTEDLRPNRVRI 60

Query: 61 FVNINSVVVSVPTVG 75
          FVN+N  VV VP  G
Sbjct: 61 FVNMNDRVVEVPRTG 75

[Close]

BLAST of CmUC07G127360.1 vs. NCBI nr
Match: KGN50840.1 (hypothetical protein Csa_004718 [Cucumis sativus])

HSP 1 Score: 81.3 bits (199), Expect = 4.0e-12
Identity = 41/69 (59.42%), Postives = 55/69 (79.71%), Query Frame = 0


Query: 7  VLPSGKSSWPELVFIESSTAVCLIERDNPNVK-VVVLLAGSPVTKDLRLDRVRVFVNINS 66
          + P GK+ WPELV ++++TA  +I++DNPNV+ VVVLLAGS  T+D+R DRV VFVNI+ 
Sbjct: 4  ICPPGKNQWPELVGVKATTAKYIIKKDNPNVENVVVLLAGSGTTEDIRCDRVWVFVNIHD 63

Query: 67 VVVSVPTVG 75
          +VV VP VG
Sbjct: 64 LVVEVPKVG 72

[Close]

BLAST of CmUC07G127360.1 vs. NCBI nr
Match: KAA0049292.1 (trypsin inhibitor [Cucumis melo var. makuwa] >TYK17266.1 trypsin inhibitor [Cucumis melo var. makuwa])

HSP 1 Score: 79.3 bits (194), Expect = 1.5e-11
Identity = 38/64 (59.38%), Postives = 48/64 (75.00%), Query Frame = 0


Query: 11 GKSSWPELVFIESSTAVCLIERDNPNVKVVVLLAGSPVTKDLRLDRVRVFVNINSVVVSV 70
          GK SWP+LV +  S A  +IER+NPNV+ VVL  G+PVTKD R +RVRV+VN N +V S 
Sbjct: 6  GKRSWPQLVGVGGSVAKAIIERENPNVRAVVLEVGTPVTKDFRCNRVRVWVNKNGLVASP 65

Query: 71 PTVG 75
          PT+G
Sbjct: 66 PTIG 69

[Close]

BLAST of CmUC07G127360.1 vs. NCBI nr
Match: KAA8527727.1 (hypothetical protein F0562_035404 [Nyssa sinensis])

HSP 1 Score: 79.3 bits (194), Expect = 1.5e-11
Identity = 37/64 (57.81%), Postives = 46/64 (71.88%), Query Frame = 0


Query: 11 GKSSWPELVFIESSTAVCLIERDNPNVKVVVLLAGSPVTKDLRLDRVRVFVNINSVVVSV 70
          GKSSWPELV ++  TA   IER+NP V  +++L GSPVT D R DRVRV+VN N +V  V
Sbjct: 7  GKSSWPELVGVQGETATATIERENPLVTALIVLEGSPVTPDFRCDRVRVYVNENGIVTLV 66

Query: 71 PTVG 75
          P +G
Sbjct: 67 PAIG 70

[Close]

BLAST of CmUC07G127360.1 vs. NCBI nr
Match: KAG6571494.1 (hypothetical protein SDJN03_28222, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 77.8 bits (190), Expect = 4.4e-11
Identity = 38/67 (56.72%), Postives = 51/67 (76.12%), Query Frame = 0


Query: 9  PSGKSSWPELVFIESSTAVCLIERDNPNVK-VVVLLAGSPVTKDLRLDRVRVFVNINSVV 68
          P GK+ WPELV I+S+ A  +I +DN NV+ V  +LAGS VT+DLR DRVR+FVNI  ++
Sbjct: 6  PPGKNRWPELVGIKSTIAEHIIRKDNSNVEHVETILAGSAVTEDLRCDRVRIFVNIKGII 65

Query: 69 VSVPTVG 75
          V +PT+G
Sbjct: 66 VQIPTIG 72

[Close]

BLAST of CmUC07G127360.1 vs. ExPASy Swiss-Prot
Match: P19873 (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 1.3e-13
Identity = 37/64 (57.81%), Postives = 46/64 (71.88%), Query Frame = 0


Query: 11 GKSSWPELVFIESSTAVCLIERDNPNVKVVVLLAGSPVTKDLRLDRVRVFVNINSVVVSV 70
          GKSSWP LV +  S A  +IER NPNVK V+L  G+PVTKD R +RVR++VN   +VVS 
Sbjct: 5  GKSSWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKDFRCNRVRIWVNKRGLVVSP 64

Query: 71 PTVG 75
          P +G
Sbjct: 65 PRIG 68

[Close]

BLAST of CmUC07G127360.1 vs. ExPASy Swiss-Prot
Match: P24076 (Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 2.3e-10
Identity = 31/64 (48.44%), Postives = 41/64 (64.06%), Query Frame = 0


Query: 11 GKSSWPELVFIESSTAVCLIERDNPNVKVVVLLAGSPVTKDLRLDRVRVFVNINSVVVSV 70
          GK SWP+LV    + A  +IER+NP V+ V++  GSPVT D R DRVRV+V    +V   
Sbjct: 5  GKRSWPQLVGSTGAAAKAVIERENPRVRAVIVRVGSPVTADFRCDRVRVWVTERGIVARP 64

Query: 71 PTVG 75
          P +G
Sbjct: 65 PAIG 68

[Close]

BLAST of CmUC07G127360.1 vs. ExPASy Swiss-Prot
Match: Q6XNP7 (Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2)

HSP 1 Score: 65.5 bits (158), Expect = 3.0e-10
Identity = 31/63 (49.21%), Postives = 42/63 (66.67%), Query Frame = 0


Query: 12 KSSWPELVFIESSTAVCLIERDNPNVKVVVLLAGSPVTKDLRLDRVRVFVNINSVVVSVP 71
          K+SWPELV      A  +I+ +N NVK +V+  G P+T+DL  +RVRVFV+ N VV  VP
Sbjct: 8  KNSWPELVGTNGDIAAGIIQTENANVKAIVVKEGLPITQDLNFNRVRVFVDENRVVTQVP 67

Query: 72 TVG 75
           +G
Sbjct: 68 AIG 70

[Close]

BLAST of CmUC07G127360.1 vs. ExPASy Swiss-Prot
Match: P16231 (Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 3.0e-10
Identity = 33/64 (51.56%), Postives = 45/64 (70.31%), Query Frame = 0


Query: 11  GKSSWPELVFIESSTAVCLIERDNPNV-KVVVLLAGSPVTKDLRLDRVRVFVNINSVVVS 70
           GK  WPEL+ + +  A  +IE++NP++  + +LL GSPVTKD R DRVR+FVNI   VV 
Sbjct: 47  GKQFWPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILGDVVQ 106

Query: 71  VPTV 74
           +P V
Sbjct: 107 IPRV 110

[Close]

BLAST of CmUC07G127360.1 vs. ExPASy Swiss-Prot
Match: P82381 (Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 1.5e-09
Identity = 32/63 (50.79%), Postives = 41/63 (65.08%), Query Frame = 0


Query: 11 GKSSWPELVFIESSTAVCLIERDNPNVKVVVLLAGSPVTKDLRLDRVRVFVNINSVVVSV 70
          GK++WPELV    + A   +ER+N NV  +VL  GS +TKD R DRV V VN + VV SV
Sbjct: 6  GKNAWPELVGKSGNMAAATVERENRNVHAIVLKEGSAMTKDFRCDRVWVIVNDHGVVTSV 65

Query: 71 PTV 74
          P +
Sbjct: 66 PHI 68

[Close]

BLAST of CmUC07G127360.1 vs. ExPASy TrEMBL
Match: A0A0A0KPI0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=3 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 6.2e-19
Identity = 55/75 (73.33%), Postives = 62/75 (82.67%), Query Frame = 0


Query: 1  MAEEKIVLP-SGKSSWPELVFIESSTAVCLIERDNPNVKVVVLLAGSPVTKDLRLDRVRV 60
          MAEEKIV P SGKSSWPELVF++SS AV  IERD P+VK VVLLAGSPVT+DLR +RVR+
Sbjct: 1  MAEEKIVFPSSGKSSWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPVTEDLRPNRVRI 60

Query: 61 FVNINSVVVSVPTVG 75
          FVN+N  VV VP  G
Sbjct: 61 FVNMNDRVVEVPRTG 75

[Close]

BLAST of CmUC07G127360.1 vs. ExPASy TrEMBL
Match: A0A0A0KME7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G286040 PE=3 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.9e-12
Identity = 41/69 (59.42%), Postives = 55/69 (79.71%), Query Frame = 0


Query: 7  VLPSGKSSWPELVFIESSTAVCLIERDNPNVK-VVVLLAGSPVTKDLRLDRVRVFVNINS 66
          + P GK+ WPELV ++++TA  +I++DNPNV+ VVVLLAGS  T+D+R DRV VFVNI+ 
Sbjct: 4  ICPPGKNQWPELVGVKATTAKYIIKKDNPNVENVVVLLAGSGTTEDIRCDRVWVFVNIHD 63

Query: 67 VVVSVPTVG 75
          +VV VP VG
Sbjct: 64 LVVEVPKVG 72

[Close]

BLAST of CmUC07G127360.1 vs. ExPASy TrEMBL
Match: A0A5A7U0I2 (Trypsin inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001190 PE=3 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 7.3e-12
Identity = 38/64 (59.38%), Postives = 48/64 (75.00%), Query Frame = 0


Query: 11 GKSSWPELVFIESSTAVCLIERDNPNVKVVVLLAGSPVTKDLRLDRVRVFVNINSVVVSV 70
          GK SWP+LV +  S A  +IER+NPNV+ VVL  G+PVTKD R +RVRV+VN N +V S 
Sbjct: 6  GKRSWPQLVGVGGSVAKAIIERENPNVRAVVLEVGTPVTKDFRCNRVRVWVNKNGLVASP 65

Query: 71 PTVG 75
          PT+G
Sbjct: 66 PTIG 69

[Close]

BLAST of CmUC07G127360.1 vs. ExPASy TrEMBL
Match: A0A5J5AE65 (Uncharacterized protein OS=Nyssa sinensis OX=561372 GN=F0562_035404 PE=3 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 7.3e-12
Identity = 37/64 (57.81%), Postives = 46/64 (71.88%), Query Frame = 0


Query: 11 GKSSWPELVFIESSTAVCLIERDNPNVKVVVLLAGSPVTKDLRLDRVRVFVNINSVVVSV 70
          GKSSWPELV ++  TA   IER+NP V  +++L GSPVT D R DRVRV+VN N +V  V
Sbjct: 7  GKSSWPELVGVQGETATATIERENPLVTALIVLEGSPVTPDFRCDRVRVYVNENGIVTLV 66

Query: 71 PTVG 75
          P +G
Sbjct: 67 PAIG 70

[Close]

BLAST of CmUC07G127360.1 vs. ExPASy TrEMBL
Match: A0A0M3SAG8 (Serine proteinase inhibitor OS=Cucumis metulifer OX=61886 PE=2 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 2.1e-11
Identity = 39/69 (56.52%), Postives = 52/69 (75.36%), Query Frame = 0


Query: 7  VLPSGKSSWPELVFIESSTAVCLIERDNPNV-KVVVLLAGSPVTKDLRLDRVRVFVNINS 66
          + P GK+ WPELV I+++TA  +I +DNP+V KV+VLLAG+  TKD+R DRV +FVNI  
Sbjct: 4  ICPPGKNQWPELVGIKATTAEYIIRKDNPSVEKVIVLLAGTGTTKDIRCDRVWLFVNIEE 63

Query: 67 VVVSVPTVG 75
           VV +P VG
Sbjct: 64 KVVEIPKVG 72

[Close]

BLAST of CmUC07G127360.1 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 68.2 bits (165), Expect = 3.2e-12
Identity = 34/63 (53.97%), Postives = 40/63 (63.49%), Query Frame = 0


Query: 12 KSSWPELVFIESSTAVCLIERDNPNVKVVVLLAGSPVTKDLRLDRVRVFVNINSVVVSVP 71
          K+SWPEL       A  +IER+NP V   V+L GSPVT D R DRVRVFV+ N +VV  P
Sbjct: 8  KNSWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFVDGNRIVVKTP 67

Query: 72 TVG 75
            G
Sbjct: 68 KSG 70

[Close]

BLAST of CmUC07G127360.1 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 55.5 bits (132), Expect = 2.2e-08
Identity = 28/64 (43.75%), Postives = 41/64 (64.06%), Query Frame = 0


Query: 11 GKSSWPELVFIESSTAVCLIERDNPNVKVVVLLAGSPVTKDLRLDRVRVFVNINSVVVSV 70
          GKSSWPEL+  +   A  +IER+NP +K V++L G+ V +     RV V+VN   +VV +
Sbjct: 36 GKSSWPELLGAKGEDAKEVIERENPKMKAVIILDGTVVPEIFICSRVYVWVNDCGIVVQI 95

Query: 71 PTVG 75
          P +G
Sbjct: 96 PIIG 99

[Close]

BLAST of CmUC07G127360.1 vs. TAIR 10
Match: AT3G46860.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 43.9 bits (102), Expect = 6.5e-05
Identity = 24/63 (38.10%), Postives = 32/63 (50.79%), Query Frame = 0


Query: 12 KSSWPELVFIESSTAVCLIERDNPNVKVVVLLAGSPVTKDLRLDRVRVFVNINSVVVSVP 71
          K  WPELV      A   IER+NPNV  +VL  G    ++   +RV V++  N  V   P
Sbjct: 23 KYRWPELVGKNGQLAKMTIERENPNVLAIVLRYGEKRIENFCCNRVFVYLGSNGQVADAP 82

Query: 72 TVG 75
           +G
Sbjct: 83 MIG 85

The following BLAST results are available for this feature:

BLAST of CmUC07G127360.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011654473.1	1.3e-18	73.33	inhibitor of trypsin and hageman factor [Cucumis sativus] >KGN49621.1 hypothetic...	[more]
KGN50840.1	4.0e-12	59.42	hypothetical protein Csa_004718 [Cucumis sativus]	[more]
KAA0049292.1	1.5e-11	59.38	trypsin inhibitor [Cucumis melo var. makuwa] >TYK17266.1 trypsin inhibitor [Cucu...	[more]
KAA8527727.1	1.5e-11	57.81	hypothetical protein F0562_035404 [Nyssa sinensis]	[more]
KAG6571494.1	4.4e-11	56.72	hypothetical protein SDJN03_28222, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of CmUC07G127360.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P19873	1.3e-13	57.81	Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]
P24076	2.3e-10	48.44	Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
Q6XNP7	3.0e-10	49.21	Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2	[more]
P16231	3.0e-10	51.56	Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1	[more]
P82381	1.5e-09	50.79	Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1	[more]

BLAST of CmUC07G127360.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KPI0	6.2e-19	73.33	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=3 SV=1	[more]
A0A0A0KME7	1.9e-12	59.42	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G286040 PE=3 SV=1	[more]
A0A5A7U0I2	7.3e-12	59.38	Trypsin inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00...	[more]
A0A5J5AE65	7.3e-12	57.81	Uncharacterized protein OS=Nyssa sinensis OX=561372 GN=F0562_035404 PE=3 SV=1	[more]
A0A0M3SAG8	2.1e-11	56.52	Serine proteinase inhibitor OS=Cucumis metulifer OX=61886 PE=2 SV=1	[more]

BLAST of CmUC07G127360.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38870.1	3.2e-12	53.97	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT5G43580.1	2.2e-08	43.75	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT3G46860.1	6.5e-05	38.10	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (USVL531) v1
Date Performed: 2022-01-31

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PRINTS	PR00292	POTATOINHBTR	coord: 51..61 score: 59.29 coord: 39..50 score: 50.72 coord: 14..27 score: 39.75
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 12..74 e-value: 5.3E-22 score: 78.3
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 6..74
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 9..74 e-value: 3.4E-24 score: 87.0
None	No IPR available	PANTHER	PTHR33091:SF83	ETHYLENE-RESPONSIVE PROTEINASE INHIBITOR 1	coord: 6..74
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 9..74

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmUC07G127360	CmUC07G127360	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmUC07G127360.1-exon	CmUC07G127360.1-exon-CmU531Chr07:774505..774535	exon
CmUC07G127360.1-exon	CmUC07G127360.1-exon-CmU531Chr07:774650..774843	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmUC07G127360.1-cds	CmUC07G127360.1-cds-CmU531Chr07:774505..774535	CDS
CmUC07G127360.1-cds	CmUC07G127360.1-cds-CmU531Chr07:774650..774843	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmUC07G127360.1	CmUC07G127360.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009611	response to wounding
biological_process	GO:0010466	negative regulation of peptidase activity
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity