Clc05G13000.1 (mRNA) Watermelon (cordophanus) v2

Overview
NameClc05G13000.1
TypemRNA
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
DescriptionAuxin-responsive GH3 family protein
LocationClcChr05: 10988964 .. 10991213 (+)
Sequence length2037
RNA-Seq ExpressionClc05G13000.1
SyntenyClc05G13000.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATTTTTATAATTTTAGAGAAAAAAAAGAAAAAAAAACCTTTTCTCCTACCCCACCCAGCTGGGTCCCGACCCAGTCAACCTTCCTACGTGGCACTTCTCCCCTTCTATATATAAGCCCTCTCCCCCCATCTCCTCCTCCGTCCCATCTCACTCATTTCCCATTCTTCTTCTTCCTCTTCTTCTTCTCCCCATGGCTGTCCATTCTTCTCTCTCTTCACCTCTCGGCCCTCCCGCCTGTGAAAACGACGCCAAGCCTCTTCAATTCATTGACCAAATGACTCGCAACGCCGACGCCGTCCAACACGCCGTCCTCGCCGAAATCTTGTCTAGAAACGCCTCCACCGAGTACCTCCGCCGTTACTCCCTCGACGGCGCCACCGATCCCCACACCTTCAAAGCTAAACTCCCCGTCATCACTTACGAGGATCTCCAGCCCGAAATCCAACGGATTGCCAGTGGAGATCGCTCGCCGATTCTCTCCTCCCATCCCATCTCCGAGTTCCTCACTAGGTATATACTCAAAATTTAACAAACATCATTAACAATACCTGAATCCATTCTTCTCGATCTTAATTGATTGATTGATTCGCTTTATTTTGCAGTTCTGGTACTTCTGCCGGTGAAAGAAAACTTATGCCGACGATTAAGGAAGAACTGGATCGTCGTCAACTTCTCTACAGTCTTCTCATGCCAGTTATGAACCTGTAAGTGAAAGCAACAATGGCGAATTTGAATTATTATTAAAATTAAAACCGCGTAAAATTAAATTTTATTTATTTATTTATTTTTTTTTTTTTTTTTTTTTTTTAAATTTTTTTGGGACAGCTATGTGCCTGGGTTGGATAAAGGCAAGGGGCTTTACTTCTTGTTTGTGAAATCGGAGACGAGGACTCCGGGTGGGCTCTTGGCCCGACCCGTTTTGACCAGCTACTACAAGAGTGACCACTTCAAGACCCGACCCTACGACCCGTTTATGGTTTACACCAGCCCTAATGAGGCCATTCTTTGCCCCGATTCCTTCCAGAGTATGTATACTCAGATGCTCTGCGGTCTTCTCCAACGCCACCAAGTCCTCCGCCTCGGCGCCGTCTTCGCCTCCGGCCTACTCCGAGCCATCCGCTTTCTTCAATTGAACTGGCAGGATCTTGCTACTGATATACGAAACGGAACGTTGAATTCGAGGATCACCGATCCGTCGTTGAGAGATTGCATCGAGAAGATCCTGAAGCCAGATGCGGAACTGGCGGATTTCGTTTCGGATGAATGTTCTAAGGAGGATTGGGAGGGAATCGTGACAAGAATTTGGCCAAAAACGAAATACCTCGACGTGATCGTCACCGGAGCGATGGCGCAGTACATTCCAACGCTCGATTACTATAGCGGCGGGTTACCTCTGGCTTGCACTATGTACGCATCATCCGAGTGTTATTTCGGATTGAATCTGAACCCTATGTGTAAACCGTCGGAGGTTTCCTACACTATAATGCCAAACATGGCGTACTTTGAATTCCTCCCTCACGAGCCGAATTCCGGTAACGATTCGCCTCCAAAACTGGTCGACCTGGTAGATGTGGAGGTCGGAAAAGAATACGAACTGGTAATCACAACATACGCGGGATTGTACCGTTACCGCGTGGGCGACATACTCCGCGTGACCGGATTCCACAACGCGGCGCCGCAGTTCCATTTCGTGAGGAGGAAGAACGTGGTGCTGAGTATTGACTCGGATAAAACGGACGAAGCAGAGTTGCAGAAGGCGGTGGAGAATGCGTCGGAGTTGTTGAAGGAATGGAAGGCGAGTGTGGTGGAGTACACGAGTTACGCAGATACAAAGACGATTCCAGGACATTACGTGATATACTGGGAATTGATGGTGAAAGAAGCGGCGGAGGAAGGGAAATGGCCGAGCAAAGAGGTGATGGAACATTGCTGCTTAGCAATGGAGGAATCACTGAACTCAGTTTACCGGCAAGGGCGAGTTGCAGATCATTCAATAGGAGCATTAGAAATCAGAGTGGTGAAGAGTGGAACATTTGAAGAACTGATGGATTACGCGATTTCAAGAGGAGCGTCTATCAACCAATATAAGGTTCCGAGGTGTGTGAATTTTACTCCCATTATGGAACTTCTCGATTCTAGAGTAGTATCGGCGCACTTTAGTCCTGCTCTGCCACATTGGACTCCAGCTAGGACACGCTAATTTCTTTTTTTCTTTTTTTCTTTTTTTCTTTTTTCTTTTCCTTTTCTTT

mRNA sequence

AATTTTTATAATTTTAGAGAAAAAAAAGAAAAAAAAACCTTTTCTCCTACCCCACCCAGCTGGGTCCCGACCCAGTCAACCTTCCTACGTGGCACTTCTCCCCTTCTATATATAAGCCCTCTCCCCCCATCTCCTCCTCCGTCCCATCTCACTCATTTCCCATTCTTCTTCTTCCTCTTCTTCTTCTCCCCATGGCTGTCCATTCTTCTCTCTCTTCACCTCTCGGCCCTCCCGCCTGTGAAAACGACGCCAAGCCTCTTCAATTCATTGACCAAATGACTCGCAACGCCGACGCCGTCCAACACGCCGTCCTCGCCGAAATCTTGTCTAGAAACGCCTCCACCGAGTACCTCCGCCGTTACTCCCTCGACGGCGCCACCGATCCCCACACCTTCAAAGCTAAACTCCCCGTCATCACTTACGAGGATCTCCAGCCCGAAATCCAACGGATTGCCAGTGGAGATCGCTCGCCGATTCTCTCCTCCCATCCCATCTCCGAGTTCCTCACTAGTTCTGGTACTTCTGCCGGTGAAAGAAAACTTATGCCGACGATTAAGGAAGAACTGGATCGTCGTCAACTTCTCTACAGTCTTCTCATGCCAGTTATGAACCTCTATGTGCCTGGGTTGGATAAAGGCAAGGGGCTTTACTTCTTGTTTGTGAAATCGGAGACGAGGACTCCGGGTGGGCTCTTGGCCCGACCCGTTTTGACCAGCTACTACAAGAGTGACCACTTCAAGACCCGACCCTACGACCCGTTTATGGTTTACACCAGCCCTAATGAGGCCATTCTTTGCCCCGATTCCTTCCAGAGTATGTATACTCAGATGCTCTGCGGTCTTCTCCAACGCCACCAAGTCCTCCGCCTCGGCGCCGTCTTCGCCTCCGGCCTACTCCGAGCCATCCGCTTTCTTCAATTGAACTGGCAGGATCTTGCTACTGATATACGAAACGGAACGTTGAATTCGAGGATCACCGATCCGTCGTTGAGAGATTGCATCGAGAAGATCCTGAAGCCAGATGCGGAACTGGCGGATTTCGTTTCGGATGAATGTTCTAAGGAGGATTGGGAGGGAATCGTGACAAGAATTTGGCCAAAAACGAAATACCTCGACGTGATCGTCACCGGAGCGATGGCGCAGTACATTCCAACGCTCGATTACTATAGCGGCGGGTTACCTCTGGCTTGCACTATGTACGCATCATCCGAGTGTTATTTCGGATTGAATCTGAACCCTATGTGTAAACCGTCGGAGGTTTCCTACACTATAATGCCAAACATGGCGTACTTTGAATTCCTCCCTCACGAGCCGAATTCCGGTAACGATTCGCCTCCAAAACTGGTCGACCTGGTAGATGTGGAGGTCGGAAAAGAATACGAACTGGTAATCACAACATACGCGGGATTGTACCGTTACCGCGTGGGCGACATACTCCGCGTGACCGGATTCCACAACGCGGCGCCGCAGTTCCATTTCGTGAGGAGGAAGAACGTGGTGCTGAGTATTGACTCGGATAAAACGGACGAAGCAGAGTTGCAGAAGGCGGTGGAGAATGCGTCGGAGTTGTTGAAGGAATGGAAGGCGAGTGTGGTGGAGTACACGAGTTACGCAGATACAAAGACGATTCCAGGACATTACGTGATATACTGGGAATTGATGGTGAAAGAAGCGGCGGAGGAAGGGAAATGGCCGAGCAAAGAGGTGATGGAACATTGCTGCTTAGCAATGGAGGAATCACTGAACTCAGTTTACCGGCAAGGGCGAGTTGCAGATCATTCAATAGGAGCATTAGAAATCAGAGTGGTGAAGAGTGGAACATTTGAAGAACTGATGGATTACGCGATTTCAAGAGGAGCGTCTATCAACCAATATAAGGTTCCGAGGTGTGTGAATTTTACTCCCATTATGGAACTTCTCGATTCTAGAGTAGTATCGGCGCACTTTAGTCCTGCTCTGCCACATTGGACTCCAGCTAGGACACGCTAATTTCTTTTTTTCTTTTTTTCTTTTTTTCTTTTTTCTTTTCCTTTTCTTT

Coding sequence (CDS)

ATGGCTGTCCATTCTTCTCTCTCTTCACCTCTCGGCCCTCCCGCCTGTGAAAACGACGCCAAGCCTCTTCAATTCATTGACCAAATGACTCGCAACGCCGACGCCGTCCAACACGCCGTCCTCGCCGAAATCTTGTCTAGAAACGCCTCCACCGAGTACCTCCGCCGTTACTCCCTCGACGGCGCCACCGATCCCCACACCTTCAAAGCTAAACTCCCCGTCATCACTTACGAGGATCTCCAGCCCGAAATCCAACGGATTGCCAGTGGAGATCGCTCGCCGATTCTCTCCTCCCATCCCATCTCCGAGTTCCTCACTAGTTCTGGTACTTCTGCCGGTGAAAGAAAACTTATGCCGACGATTAAGGAAGAACTGGATCGTCGTCAACTTCTCTACAGTCTTCTCATGCCAGTTATGAACCTCTATGTGCCTGGGTTGGATAAAGGCAAGGGGCTTTACTTCTTGTTTGTGAAATCGGAGACGAGGACTCCGGGTGGGCTCTTGGCCCGACCCGTTTTGACCAGCTACTACAAGAGTGACCACTTCAAGACCCGACCCTACGACCCGTTTATGGTTTACACCAGCCCTAATGAGGCCATTCTTTGCCCCGATTCCTTCCAGAGTATGTATACTCAGATGCTCTGCGGTCTTCTCCAACGCCACCAAGTCCTCCGCCTCGGCGCCGTCTTCGCCTCCGGCCTACTCCGAGCCATCCGCTTTCTTCAATTGAACTGGCAGGATCTTGCTACTGATATACGAAACGGAACGTTGAATTCGAGGATCACCGATCCGTCGTTGAGAGATTGCATCGAGAAGATCCTGAAGCCAGATGCGGAACTGGCGGATTTCGTTTCGGATGAATGTTCTAAGGAGGATTGGGAGGGAATCGTGACAAGAATTTGGCCAAAAACGAAATACCTCGACGTGATCGTCACCGGAGCGATGGCGCAGTACATTCCAACGCTCGATTACTATAGCGGCGGGTTACCTCTGGCTTGCACTATGTACGCATCATCCGAGTGTTATTTCGGATTGAATCTGAACCCTATGTGTAAACCGTCGGAGGTTTCCTACACTATAATGCCAAACATGGCGTACTTTGAATTCCTCCCTCACGAGCCGAATTCCGGTAACGATTCGCCTCCAAAACTGGTCGACCTGGTAGATGTGGAGGTCGGAAAAGAATACGAACTGGTAATCACAACATACGCGGGATTGTACCGTTACCGCGTGGGCGACATACTCCGCGTGACCGGATTCCACAACGCGGCGCCGCAGTTCCATTTCGTGAGGAGGAAGAACGTGGTGCTGAGTATTGACTCGGATAAAACGGACGAAGCAGAGTTGCAGAAGGCGGTGGAGAATGCGTCGGAGTTGTTGAAGGAATGGAAGGCGAGTGTGGTGGAGTACACGAGTTACGCAGATACAAAGACGATTCCAGGACATTACGTGATATACTGGGAATTGATGGTGAAAGAAGCGGCGGAGGAAGGGAAATGGCCGAGCAAAGAGGTGATGGAACATTGCTGCTTAGCAATGGAGGAATCACTGAACTCAGTTTACCGGCAAGGGCGAGTTGCAGATCATTCAATAGGAGCATTAGAAATCAGAGTGGTGAAGAGTGGAACATTTGAAGAACTGATGGATTACGCGATTTCAAGAGGAGCGTCTATCAACCAATATAAGGTTCCGAGGTGTGTGAATTTTACTCCCATTATGGAACTTCTCGATTCTAGAGTAGTATCGGCGCACTTTAGTCCTGCTCTGCCACATTGGACTCCAGCTAGGACACGCTAA

Protein sequence

MAVHSSLSSPLGPPACENDAKPLQFIDQMTRNADAVQHAVLAEILSRNASTEYLRRYSLDGATDPHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSDHFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRFLQLNWQDLATDIRNGTLNSRITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRIWPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTIMPNMAYFEFLPHEPNSGNDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEWKASVVEYTSYADTKTIPGHYVIYWELMVKEAAEEGKWPSKEVMEHCCLAMEESLNSVYRQGRVADHSIGALEIRVVKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARTR
Homology
BLAST of Clc05G13000.1 vs. NCBI nr
Match: XP_038877583.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Benincasa hispida])

HSP 1 Score: 1196.8 bits (3095), Expect = 0.0e+00
Identity = 589/598 (98.49%), Postives = 592/598 (99.00%), Query Frame = 0

Query: 1   MAVHSSLSSPLGPPACENDAKPLQFIDQMTRNADAVQHAVLAEILSRNASTEYLRRYSLD 60
           MAVHSSLSSPLGPPACENDAKPLQFIDQMTRNADAVQH VLAEILSRNASTEYLRRYSLD
Sbjct: 1   MAVHSSLSSPLGPPACENDAKPLQFIDQMTRNADAVQHTVLAEILSRNASTEYLRRYSLD 60

Query: 61  GATDPHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDP TFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWQDLATDIRNGTLNSRITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300
           LQLNWQDLATDIRNGTLNSRITDPSLRDCIEKILKPDAELADF+SDECSKEDWEGIVTRI
Sbjct: 241 LQLNWQDLATDIRNGTLNSRITDPSLRDCIEKILKPDAELADFLSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGNDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPHEPNSGNDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNSGNDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEWKASVVEYTSYADTKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVE ASELLKEWK SVVEYTSYA+TKTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVEKASELLKEWKTSVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELMVKEAAEEGKWPSKEVMEHCCLAMEESLNSVYRQGRVADHSIGALEIRVVK 540
           GHYVIYWELMVKEA EEGKWPSKEVME CCLAMEESLNSVYRQGRVAD+SIGALEIRVVK
Sbjct: 481 GHYVIYWELMVKEATEEGKWPSKEVMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVK 540

Query: 541 SGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARTR 599
           SGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARTR
Sbjct: 541 SGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARTR 598

BLAST of Clc05G13000.1 vs. NCBI nr
Match: XP_008465614.1 (PREDICTED: probable indole-3-acetic acid-amido synthetase GH3.1 [Cucumis melo] >KAA0037204.1 putative indole-3-acetic acid-amido synthetase GH3.1 [Cucumis melo var. makuwa])

HSP 1 Score: 1164.4 bits (3011), Expect = 0.0e+00
Identity = 574/599 (95.83%), Postives = 585/599 (97.66%), Query Frame = 0

Query: 1   MAVHSSLSSPLGPPACENDAKPLQFIDQMTRNADAVQHAVLAEILSRNASTEYLRRYSLD 60
           MAVHSSLSSPLGPPACE DAKPLQFID+MTRNADAVQH VLAEILSRNASTEYLRRYSLD
Sbjct: 1   MAVHSSLSSPLGPPACETDAKPLQFIDEMTRNADAVQHTVLAEILSRNASTEYLRRYSLD 60

Query: 61  GATDPHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDP TFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQR+QVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRNQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWQDLATDIRNGTLNSRITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300
           LQLNW DLATDIRNGTLNSRITDPSLRDCI KILKPD++LADFVSDECSKEDWEGIVTRI
Sbjct: 241 LQLNWHDLATDIRNGTLNSRITDPSLRDCILKILKPDSQLADFVSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLD+YSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDFYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGNDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLP EPNSG+DS PKLVDLVDVE+GKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPLEPNSGSDSSPKLVDLVDVEIGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEWKASVVEYTSYADTKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEWKASVVEYTSYA+TKTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELMVKEAAEE---GKWPSKEVMEHCCLAMEESLNSVYRQGRVADHSIGALEIR 540
           GHYVIYWELMVKEA ++   GKWPSKEVME CCL MEES+NSVYRQGRVADHSIGALEIR
Sbjct: 481 GHYVIYWELMVKEAGDQEGKGKWPSKEVMEECCLRMEESMNSVYRQGRVADHSIGALEIR 540

Query: 541 VVKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPAR 597
           VVKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVS HFSPALPHWTPAR
Sbjct: 541 VVKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSTHFSPALPHWTPAR 599

BLAST of Clc05G13000.1 vs. NCBI nr
Match: XP_022922958.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita moschata])

HSP 1 Score: 1162.9 bits (3007), Expect = 0.0e+00
Identity = 573/598 (95.82%), Postives = 586/598 (97.99%), Query Frame = 0

Query: 1   MAVHSSLSSPLGPPACENDAKPLQFIDQMTRNADAVQHAVLAEILSRNASTEYLRRYSLD 60
           MAV SSLSSPLGPPACE DAK LQFI+QMTRNADAVQ  VLAEIL+RNA+TEYLRRYSLD
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60

Query: 61  GATDPHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDPHTFK KLPVITYEDL+P+IQRIA+GDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDPF+VYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWQDLATDIRNGTLNSRITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300
           LQLNWQDLA DIRNGTLNS+ITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI
Sbjct: 241 LQLNWQDLANDIRNGTLNSKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGNDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPHEPN+ +DSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEWKASVVEYTSYADTKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENAS+LLKEWKASVVEYTSYA+TKTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELMVKEAAEEGKWPSKEVMEHCCLAMEESLNSVYRQGRVADHSIGALEIRVVK 540
           GHYVIYWELMVKEA  EGKWPSKEVME CCLAMEESLNSVYRQGRVADHSIGALEIRVVK
Sbjct: 481 GHYVIYWELMVKEA--EGKWPSKEVMEECCLAMEESLNSVYRQGRVADHSIGALEIRVVK 540

Query: 541 SGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARTR 599
           +GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALP WTPARTR
Sbjct: 541 NGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARTR 596

BLAST of Clc05G13000.1 vs. NCBI nr
Match: KAG6576928.1 (putative indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014954.1 putative indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1162.1 bits (3005), Expect = 0.0e+00
Identity = 572/598 (95.65%), Postives = 586/598 (97.99%), Query Frame = 0

Query: 1   MAVHSSLSSPLGPPACENDAKPLQFIDQMTRNADAVQHAVLAEILSRNASTEYLRRYSLD 60
           MAV SSLSSPLGPPACE DAK LQFI+QMTRNADAVQ  VLAEIL+RNA+TEYLRRYSLD
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60

Query: 61  GATDPHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDPHTFK KLPVITYEDL+P+IQRIA+GDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDPF+VYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGA+FASGLLRAIRF
Sbjct: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAIFASGLLRAIRF 240

Query: 241 LQLNWQDLATDIRNGTLNSRITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300
           LQLNWQDLA DIRNGTLNS+ITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI
Sbjct: 241 LQLNWQDLANDIRNGTLNSKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGNDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPHEPN+ +DSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEWKASVVEYTSYADTKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENAS+LLKEWKASVVEYTSYA+TKTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELMVKEAAEEGKWPSKEVMEHCCLAMEESLNSVYRQGRVADHSIGALEIRVVK 540
           GHYVIYWELMVKEA  EGKWPSKEVME CCLAMEESLNSVYRQGRVADHSIGALEIRVVK
Sbjct: 481 GHYVIYWELMVKEA--EGKWPSKEVMEDCCLAMEESLNSVYRQGRVADHSIGALEIRVVK 540

Query: 541 SGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARTR 599
           +GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALP WTPARTR
Sbjct: 541 NGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARTR 596

BLAST of Clc05G13000.1 vs. NCBI nr
Match: XP_022985331.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita maxima])

HSP 1 Score: 1160.2 bits (3000), Expect = 0.0e+00
Identity = 570/598 (95.32%), Postives = 585/598 (97.83%), Query Frame = 0

Query: 1   MAVHSSLSSPLGPPACENDAKPLQFIDQMTRNADAVQHAVLAEILSRNASTEYLRRYSLD 60
           MAV SSLSSPLGPPACE DAK LQFI+QMTRNADAVQ  VLAEIL+RNA+TEYLRRYSLD
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60

Query: 61  GATDPHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           G TDPHTFK KLPVITYEDL+P+IQRIA+GDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GTTDPHTFKGKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDPF+VYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWQDLATDIRNGTLNSRITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300
           LQLNWQDLA DIRNGTLN++ITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI
Sbjct: 241 LQLNWQDLANDIRNGTLNTKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGNDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPHEPN+ +DSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEWKASVVEYTSYADTKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENAS+LLKEWKASVVEYTSYA+TKTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELMVKEAAEEGKWPSKEVMEHCCLAMEESLNSVYRQGRVADHSIGALEIRVVK 540
           GHYVIYWELMVKEA  EGKWPSKEVME CCLAMEESLNSVYRQGRVADHSIGALEIRVVK
Sbjct: 481 GHYVIYWELMVKEA--EGKWPSKEVMEECCLAMEESLNSVYRQGRVADHSIGALEIRVVK 540

Query: 541 SGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARTR 599
           +GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRV+SAHFSPALP WTPARTR
Sbjct: 541 NGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVISAHFSPALPRWTPARTR 596

BLAST of Clc05G13000.1 vs. ExPASy Swiss-Prot
Match: O82333 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=3702 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 966.1 bits (2496), Expect = 1.9e-280
Identity = 477/599 (79.63%), Postives = 527/599 (87.98%), Query Frame = 0

Query: 1   MAVHSSLSSPLGPPACENDAKPLQFIDQMTRNADAVQHAVLAEILSRNASTEYLRRYSLD 60
           MAV S+LSSPLGPPACE DAK L+FI++MTRNAD VQ  +LAEIL+RNA TEYLRR++L 
Sbjct: 1   MAVDSNLSSPLGPPACEKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLC 60

Query: 61  GATDPHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATD  TFK K+PVITYEDLQPEIQRIA GDRSPILS+HPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDRDTFKTKIPVITYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKG+YFLFVKSET+TPGGL ARPVLTSYYKS+
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSE 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240
           HF++RPYDP+ VYTSPNEAILCPDSFQSMYTQMLCGLL R  VLR+GAVFASGLLRAIRF
Sbjct: 181 HFRSRPYDPYNVYTSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRF 240

Query: 241 LQLNWQDLATDIRNGTLNSRITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300
           LQL+W   A DI  G L+S ITDPS+R C+  ILKPD  LA+F+  EC  ++WE I+TRI
Sbjct: 241 LQLHWSRFAHDIELGCLDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WP TKYLDVIVTGAMAQYIPTL+YYSGGLP+ACTMYASSECYFGLNLNPM KPSEVSYTI
Sbjct: 301 WPNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGNDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEF+P           K V+LVDV +GKEYELV+TTYAGL RYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFIPL-------GGTKAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELL-KEWKASVVEYTSYADTKTI 480
           HN+APQFHFVRRKNV+LSIDSDKTDE+ELQKAVENAS +L +E  + V EYTSYADT TI
Sbjct: 421 HNSAPQFHFVRRKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTI 480

Query: 481 PGHYVIYWELMVKEAAEEGKWPSKEVMEHCCLAMEESLNSVYRQGRVADHSIGALEIRVV 540
           PGHYV+YWEL+V++ A +   PS E +  CCL MEESLNSVYRQ RVAD+S+G LEIRVV
Sbjct: 481 PGHYVLYWELLVRDGARQ---PSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVV 540

Query: 541 KSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARTR 599
           ++GTFEELMDYAISRGASINQYKVPRCVNFTPI+ELLDSRVVSAHFSP+LPHWTP R R
Sbjct: 541 RNGTFEELMDYAISRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPERRR 589

BLAST of Clc05G13000.1 vs. ExPASy Swiss-Prot
Match: O22190 (Indole-3-acetic acid-amido synthetase GH3.3 OS=Arabidopsis thaliana OX=3702 GN=GH3.3 PE=1 SV=1)

HSP 1 Score: 906.7 bits (2342), Expect = 1.3e-262
Identity = 454/600 (75.67%), Postives = 512/600 (85.33%), Query Frame = 0

Query: 1   MAVHSSLSSPLGPPACENDAKPLQFIDQMTRNADAVQHAVLAEILSRNASTEYLRRYSLD 60
           M V S+L SP+       D K L+FI++MTRN D VQ  V+ EILSRN+ TEYL+R+ L 
Sbjct: 1   MTVDSALRSPMMHSPSTKDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLK 60

Query: 61  GATDPHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           G TD  TFK K+PV+ Y+DL+PEIQRIA+GDRS ILSS+PI+EFLTSSGTSAGERKLMPT
Sbjct: 61  GFTDRKTFKTKVPVVIYDDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           I E++DRRQLLYSLLMPVMNLYVPGLDKGK LYFLFVK+E++TPGGL ARPVLTSYYKS+
Sbjct: 121 IDEDMDRRQLLYSLLMPVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSE 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240
            FK RP DP+ VYTSPNEAILCPDS QSMYTQMLCGLL RH+VLRLGAVFASGLLRAI F
Sbjct: 181 QFKRRPNDPYNVYTSPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGF 240

Query: 241 LQLNWQDLATDIRNGTLNSRITDPSLRDCIEKIL-KPDAELADFVSDECSKED-WEGIVT 300
           LQ NW++LA DI  GTL+SRI+DP++++ + KIL KPD ELADF++  C +++ WEGI+T
Sbjct: 241 LQTNWKELADDISTGTLSSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIIT 300

Query: 301 RIWPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSY 360
           +IWP TKYLDVIVTGAMAQYIP L+YYSGGLP+ACTMYASSE YFG+NL PMCKPSEVSY
Sbjct: 301 KIWPNTKYLDVIVTGAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSY 360

Query: 361 TIMPNMAYFEFLPHEPNSGNDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVT 420
           TIMPNMAYFEFLPH       S  +LV+L DVEVGKEYELVITTYAGL RYRVGDIL+VT
Sbjct: 361 TIMPNMAYFEFLPHHEVPTEKS--ELVELADVEVGKEYELVITTYAGLNRYRVGDILQVT 420

Query: 421 GFHNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEWKASVVEYTSYADTKT 480
           GF+N+APQF FVRRKNV+LSI+SDKTDEAELQ AVENAS LL E    V+EYTSYA+TKT
Sbjct: 421 GFYNSAPQFKFVRRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKT 480

Query: 481 IPGHYVIYWELMVKEAAEEGKWPSKEVMEHCCLAMEESLNSVYRQGRVADHSIGALEIRV 540
           IPGHYVIYWEL+VK+       P+ EVM  CCL MEESLNSVYRQ RVAD SIG LEIRV
Sbjct: 481 IPGHYVIYWELLVKDQTNP---PNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRV 540

Query: 541 VKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARTR 599
           VK+GTFEELMDYAISRGASINQYKVPRCV+FTPIMELLDSRVVS HFSPALPHW+P R R
Sbjct: 541 VKNGTFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHWSPERRR 595

BLAST of Clc05G13000.1 vs. ExPASy Swiss-Prot
Match: A3BLS0 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=39946 GN=GH3.8 PE=1 SV=2)

HSP 1 Score: 896.3 bits (2315), Expect = 1.8e-259
Identity = 445/593 (75.04%), Postives = 495/593 (83.47%), Query Frame = 0

Query: 5   SSLSSPLGPPACENDAKPLQFIDQMTRNADAVQHAVLAEILSRNASTEYLRRYSLDGATD 64
           ++L +P      E D + L+FID+MT N DAVQ  VL EIL RNA TEYL +  LDGATD
Sbjct: 12  TALRTPAAGAVKEGDVEKLRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATD 71

Query: 65  PHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPTIKEE 124
              F+AK+PV++Y+DLQP IQRIA+GDRSPILS+HP+SEFLTSSGTSAGERKLMPTI +E
Sbjct: 72  RAAFRAKVPVVSYDDLQPYIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDE 131

Query: 125 LDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSDHFKT 184
           LDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSET+TPGGL ARPVLTSYYKSDHFK 
Sbjct: 132 LDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKN 191

Query: 185 RPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRFLQLN 244
           RPYDP+  YTSP  AILC D+FQSMY QM+CGL QR+ VLRLGAVFASGLLRAIRFLQLN
Sbjct: 192 RPYDPYHNYTSPTAAILCADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLN 251

Query: 245 WQDLATDIRNGTLNSRITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRIWPKT 304
           W+ LA DI +G L  R+TDPS+R+ +  IL PD ELA  +  ECSK DW GI+TR+WP T
Sbjct: 252 WEQLADDIESGELTPRVTDPSVREAVAAILLPDPELAKLIRAECSKGDWAGIITRVWPNT 311

Query: 305 KYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTIMPNM 364
           KYLDVIVTGAMAQYIPTL++YSGGLP+ACTMYASSECYFGLNL PMC PSEVSYTIMPNM
Sbjct: 312 KYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNM 371

Query: 365 AYFEFLP-HEPNSGNDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNA 424
            YFEFLP  E  + +    +LVDL  VEVG+EYELVITTYAGL RYRVGD+LRVTGFHNA
Sbjct: 372 GYFEFLPVDETGAASGDATQLVDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNA 431

Query: 425 APQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEWKASVVEYTSYADTKTIPGHY 484
           APQF FVRRKNV+LSI+SDKTDEAELQ+AVE AS LL+   ASVVEYTS A TK IPGHY
Sbjct: 432 APQFRFVRRKNVLLSIESDKTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHY 491

Query: 485 VIYWELMVKEAAEEGKWPSKEVMEHCCLAMEESLNSVYRQGRVADHSIGALEIRVVKSGT 544
           VIYWEL+ K A         + +  CCL MEE+LN+VYRQ RVAD SIG LEIRVV+ GT
Sbjct: 492 VIYWELLTKGAG--ATVVDADTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGT 551

Query: 545 FEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPAR 597
           FEELMDYAISRGASINQYKVPRCV F PI+ELLDSRVVS+HFSPALPHWTPAR
Sbjct: 552 FEELMDYAISRGASINQYKVPRCVTFPPIVELLDSRVVSSHFSPALPHWTPAR 602

BLAST of Clc05G13000.1 vs. ExPASy Swiss-Prot
Match: Q0D4Z6 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.8 PE=1 SV=1)

HSP 1 Score: 896.3 bits (2315), Expect = 1.8e-259
Identity = 445/593 (75.04%), Postives = 495/593 (83.47%), Query Frame = 0

Query: 5   SSLSSPLGPPACENDAKPLQFIDQMTRNADAVQHAVLAEILSRNASTEYLRRYSLDGATD 64
           ++L +P      E D + L+FID+MT N DAVQ  VL EIL RNA TEYL +  LDGATD
Sbjct: 12  TALRTPAAGAVKEGDVEKLRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATD 71

Query: 65  PHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPTIKEE 124
              F+AK+PV++Y+DLQP IQRIA+GDRSPILS+HP+SEFLTSSGTSAGERKLMPTI +E
Sbjct: 72  RAAFRAKVPVVSYDDLQPYIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDE 131

Query: 125 LDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSDHFKT 184
           LDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSET+TPGGL ARPVLTSYYKSDHFK 
Sbjct: 132 LDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKN 191

Query: 185 RPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRFLQLN 244
           RPYDP+  YTSP  AILC D+FQSMY QM+CGL QR+ VLRLGAVFASGLLRAIRFLQLN
Sbjct: 192 RPYDPYHNYTSPTAAILCADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLN 251

Query: 245 WQDLATDIRNGTLNSRITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRIWPKT 304
           W+ LA DI +G L  R+TDPS+R+ +  IL PD ELA  +  ECSK DW GI+TR+WP T
Sbjct: 252 WEQLADDIESGELTPRVTDPSVREAVAAILLPDPELAKLIRAECSKGDWAGIITRVWPNT 311

Query: 305 KYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTIMPNM 364
           KYLDVIVTGAMAQYIPTL++YSGGLP+ACTMYASSECYFGLNL PMC PSEVSYTIMPNM
Sbjct: 312 KYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNM 371

Query: 365 AYFEFLP-HEPNSGNDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNA 424
            YFEFLP  E  + +    +LVDL  VEVG+EYELVITTYAGL RYRVGD+LRVTGFHNA
Sbjct: 372 GYFEFLPVDETGAASGDATQLVDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNA 431

Query: 425 APQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEWKASVVEYTSYADTKTIPGHY 484
           APQF FVRRKNV+LSI+SDKTDEAELQ+AVE AS LL+   ASVVEYTS A TK IPGHY
Sbjct: 432 APQFRFVRRKNVLLSIESDKTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHY 491

Query: 485 VIYWELMVKEAAEEGKWPSKEVMEHCCLAMEESLNSVYRQGRVADHSIGALEIRVVKSGT 544
           VIYWEL+ K A         + +  CCL MEE+LN+VYRQ RVAD SIG LEIRVV+ GT
Sbjct: 492 VIYWELLTKGAG--ATVVDADTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGT 551

Query: 545 FEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPAR 597
           FEELMDYAISRGASINQYKVPRCV F PI+ELLDSRVVS+HFSPALPHWTPAR
Sbjct: 552 FEELMDYAISRGASINQYKVPRCVTFPPIVELLDSRVVSSHFSPALPHWTPAR 602

BLAST of Clc05G13000.1 vs. ExPASy Swiss-Prot
Match: P0C0M2 (Probable indole-3-acetic acid-amido synthetase GH3.2 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.2 PE=2 SV=1)

HSP 1 Score: 884.4 bits (2284), Expect = 7.1e-256
Identity = 433/591 (73.27%), Postives = 504/591 (85.28%), Query Frame = 0

Query: 15  ACENDAKPLQFIDQMTRNADAVQHAVLAEILSRNASTEYLRRYSLDGATDPHTFKAKLPV 74
           ACE DA+ L+FI++MTR  DAVQ  VLA IL+RN   EYLRR+ ++G TD   FKA++PV
Sbjct: 25  ACERDAEKLEFIEEMTRGFDAVQERVLAAILARNNGAEYLRRHGMEGRTDREAFKARVPV 84

Query: 75  ITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSL 134
           +TYEDL+PEI+RIA+GDRS I+SSHPI+EFLTSSGTSAGERKLMPTI++ELDRRQ+LYSL
Sbjct: 85  VTYEDLRPEIERIANGDRSNIISSHPITEFLTSSGTSAGERKLMPTIEDELDRRQMLYSL 144

Query: 135 LMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSDHFKTRPYDPFMVYT 194
           LMPVMNLYVPGLDKGKGLYFLF+KSET+TPGGL ARPVLTSYYKSDHFK RP+DP+ VYT
Sbjct: 145 LMPVMNLYVPGLDKGKGLYFLFIKSETKTPGGLPARPVLTSYYKSDHFKHRPFDPYNVYT 204

Query: 195 SPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRFLQLNWQDLATDIRN 254
           SP  AILC D+FQSMY QMLCGL+ R +VLR+GAVFASGLLRAIRFLQL+W++LA DIR 
Sbjct: 205 SPTAAILCTDAFQSMYAQMLCGLVARAEVLRVGAVFASGLLRAIRFLQLHWRELAHDIRT 264

Query: 255 GTLNSRITDPSLRDCIEKIL-KPDAELADFVSDECSKEDWEGIVTRIWPKTKYLDVIVTG 314
           GTL++++T+PS+RD + ++L  PDAELA FV  EC K+ WEGI+TR+WP TKYLDVIVTG
Sbjct: 265 GTLSAKVTEPSIRDAVAEVLAAPDAELAAFVEAECGKDKWEGIITRMWPNTKYLDVIVTG 324

Query: 315 AMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTIMPNMAYFEFLPHE 374
           AMAQYIPTL +YSGGLP+ACTMYASSECYFGLNL PMC PSEVSYTIMPNM YFE +PH+
Sbjct: 325 AMAQYIPTLKFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFELMPHD 384

Query: 375 PNS----GNDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNAAPQFHF 434
           P++     +  PP+LVDL D EVG+EYELVITTYAGL RYRVGDIL+VTGFHNAAPQF F
Sbjct: 385 PDAPPLPRDAPPPRLVDLADAEVGREYELVITTYAGLCRYRVGDILQVTGFHNAAPQFRF 444

Query: 435 VRRKNVVLSIDSDKTDEAELQKAVENASELLKEWKASVVEYTSYADTKTIPGHYVIYWEL 494
           VRRKNV+LSIDSDKTDEAELQ AVE AS LL  + AS+VEYTS AD  TIPGHYV+YWEL
Sbjct: 445 VRRKNVLLSIDSDKTDEAELQAAVERASALLSPYGASIVEYTSQADATTIPGHYVVYWEL 504

Query: 495 MVKEAAEEGKWP------SKEVMEHCCLAMEESLNSVYRQGRVADHSIGALEIRVVKSGT 554
           MV+E    G WP       + V E CCL MEE+LN+VYRQGR  + +IG LEIRVV++GT
Sbjct: 505 MVREG---GAWPPPAEEEGRGVFERCCLEMEEALNAVYRQGRNGE-AIGPLEIRVVRAGT 564

Query: 555 FEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTP 595
           FEE+MDYAISRGASINQYK PRCV+F PI+ELL+SRV+S HFSPA P ++P
Sbjct: 565 FEEVMDYAISRGASINQYKAPRCVSFGPIIELLNSRVISKHFSPACPKYSP 611

BLAST of Clc05G13000.1 vs. ExPASy TrEMBL
Match: A0A5A7T148 (Putative indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold379G001170 PE=3 SV=1)

HSP 1 Score: 1164.4 bits (3011), Expect = 0.0e+00
Identity = 574/599 (95.83%), Postives = 585/599 (97.66%), Query Frame = 0

Query: 1   MAVHSSLSSPLGPPACENDAKPLQFIDQMTRNADAVQHAVLAEILSRNASTEYLRRYSLD 60
           MAVHSSLSSPLGPPACE DAKPLQFID+MTRNADAVQH VLAEILSRNASTEYLRRYSLD
Sbjct: 1   MAVHSSLSSPLGPPACETDAKPLQFIDEMTRNADAVQHTVLAEILSRNASTEYLRRYSLD 60

Query: 61  GATDPHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDP TFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQR+QVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRNQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWQDLATDIRNGTLNSRITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300
           LQLNW DLATDIRNGTLNSRITDPSLRDCI KILKPD++LADFVSDECSKEDWEGIVTRI
Sbjct: 241 LQLNWHDLATDIRNGTLNSRITDPSLRDCILKILKPDSQLADFVSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLD+YSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDFYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGNDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLP EPNSG+DS PKLVDLVDVE+GKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPLEPNSGSDSSPKLVDLVDVEIGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEWKASVVEYTSYADTKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEWKASVVEYTSYA+TKTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELMVKEAAEE---GKWPSKEVMEHCCLAMEESLNSVYRQGRVADHSIGALEIR 540
           GHYVIYWELMVKEA ++   GKWPSKEVME CCL MEES+NSVYRQGRVADHSIGALEIR
Sbjct: 481 GHYVIYWELMVKEAGDQEGKGKWPSKEVMEECCLRMEESMNSVYRQGRVADHSIGALEIR 540

Query: 541 VVKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPAR 597
           VVKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVS HFSPALPHWTPAR
Sbjct: 541 VVKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSTHFSPALPHWTPAR 599

BLAST of Clc05G13000.1 vs. ExPASy TrEMBL
Match: A0A1S3CP72 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo OX=3656 GN=LOC103503255 PE=3 SV=1)

HSP 1 Score: 1164.4 bits (3011), Expect = 0.0e+00
Identity = 574/599 (95.83%), Postives = 585/599 (97.66%), Query Frame = 0

Query: 1   MAVHSSLSSPLGPPACENDAKPLQFIDQMTRNADAVQHAVLAEILSRNASTEYLRRYSLD 60
           MAVHSSLSSPLGPPACE DAKPLQFID+MTRNADAVQH VLAEILSRNASTEYLRRYSLD
Sbjct: 1   MAVHSSLSSPLGPPACETDAKPLQFIDEMTRNADAVQHTVLAEILSRNASTEYLRRYSLD 60

Query: 61  GATDPHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDP TFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQR+QVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRNQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWQDLATDIRNGTLNSRITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300
           LQLNW DLATDIRNGTLNSRITDPSLRDCI KILKPD++LADFVSDECSKEDWEGIVTRI
Sbjct: 241 LQLNWHDLATDIRNGTLNSRITDPSLRDCILKILKPDSQLADFVSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLD+YSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDFYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGNDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLP EPNSG+DS PKLVDLVDVE+GKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPLEPNSGSDSSPKLVDLVDVEIGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEWKASVVEYTSYADTKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEWKASVVEYTSYA+TKTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELMVKEAAEE---GKWPSKEVMEHCCLAMEESLNSVYRQGRVADHSIGALEIR 540
           GHYVIYWELMVKEA ++   GKWPSKEVME CCL MEES+NSVYRQGRVADHSIGALEIR
Sbjct: 481 GHYVIYWELMVKEAGDQEGKGKWPSKEVMEECCLRMEESMNSVYRQGRVADHSIGALEIR 540

Query: 541 VVKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPAR 597
           VVKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVS HFSPALPHWTPAR
Sbjct: 541 VVKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSTHFSPALPHWTPAR 599

BLAST of Clc05G13000.1 vs. ExPASy TrEMBL
Match: A0A6J1E8A0 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita moschata OX=3662 GN=LOC111430783 PE=3 SV=1)

HSP 1 Score: 1162.9 bits (3007), Expect = 0.0e+00
Identity = 573/598 (95.82%), Postives = 586/598 (97.99%), Query Frame = 0

Query: 1   MAVHSSLSSPLGPPACENDAKPLQFIDQMTRNADAVQHAVLAEILSRNASTEYLRRYSLD 60
           MAV SSLSSPLGPPACE DAK LQFI+QMTRNADAVQ  VLAEIL+RNA+TEYLRRYSLD
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60

Query: 61  GATDPHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDPHTFK KLPVITYEDL+P+IQRIA+GDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDPF+VYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWQDLATDIRNGTLNSRITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300
           LQLNWQDLA DIRNGTLNS+ITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI
Sbjct: 241 LQLNWQDLANDIRNGTLNSKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGNDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPHEPN+ +DSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEWKASVVEYTSYADTKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENAS+LLKEWKASVVEYTSYA+TKTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELMVKEAAEEGKWPSKEVMEHCCLAMEESLNSVYRQGRVADHSIGALEIRVVK 540
           GHYVIYWELMVKEA  EGKWPSKEVME CCLAMEESLNSVYRQGRVADHSIGALEIRVVK
Sbjct: 481 GHYVIYWELMVKEA--EGKWPSKEVMEECCLAMEESLNSVYRQGRVADHSIGALEIRVVK 540

Query: 541 SGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARTR 599
           +GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALP WTPARTR
Sbjct: 541 NGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARTR 596

BLAST of Clc05G13000.1 vs. ExPASy TrEMBL
Match: A0A6J1J7V1 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita maxima OX=3661 GN=LOC111483372 PE=3 SV=1)

HSP 1 Score: 1160.2 bits (3000), Expect = 0.0e+00
Identity = 570/598 (95.32%), Postives = 585/598 (97.83%), Query Frame = 0

Query: 1   MAVHSSLSSPLGPPACENDAKPLQFIDQMTRNADAVQHAVLAEILSRNASTEYLRRYSLD 60
           MAV SSLSSPLGPPACE DAK LQFI+QMTRNADAVQ  VLAEIL+RNA+TEYLRRYSLD
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60

Query: 61  GATDPHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           G TDPHTFK KLPVITYEDL+P+IQRIA+GDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GTTDPHTFKGKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDPF+VYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWQDLATDIRNGTLNSRITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300
           LQLNWQDLA DIRNGTLN++ITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI
Sbjct: 241 LQLNWQDLANDIRNGTLNTKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGNDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPHEPN+ +DSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEWKASVVEYTSYADTKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENAS+LLKEWKASVVEYTSYA+TKTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELMVKEAAEEGKWPSKEVMEHCCLAMEESLNSVYRQGRVADHSIGALEIRVVK 540
           GHYVIYWELMVKEA  EGKWPSKEVME CCLAMEESLNSVYRQGRVADHSIGALEIRVVK
Sbjct: 481 GHYVIYWELMVKEA--EGKWPSKEVMEECCLAMEESLNSVYRQGRVADHSIGALEIRVVK 540

Query: 541 SGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARTR 599
           +GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRV+SAHFSPALP WTPARTR
Sbjct: 541 NGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVISAHFSPALPRWTPARTR 596

BLAST of Clc05G13000.1 vs. ExPASy TrEMBL
Match: A0A0A0L698 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G198490 PE=3 SV=1)

HSP 1 Score: 1141.3 bits (2951), Expect = 0.0e+00
Identity = 562/595 (94.45%), Postives = 578/595 (97.14%), Query Frame = 0

Query: 1   MAVHSSLSSPLGPPACENDAKPLQFIDQMTRNADAVQHAVLAEILSRNASTEYLRRYSLD 60
           MAVHSSLSSPLGPPACE D+KPLQFID+MTRNA AVQH +L+EILSRNASTEYLRRYSLD
Sbjct: 1   MAVHSSLSSPLGPPACERDSKPLQFIDEMTRNAHAVQHTLLSEILSRNASTEYLRRYSLD 60

Query: 61  GATDPHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDP TFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQR+QVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRNQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWQDLATDIRNGTLNSRITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300
           LQLNW DLATDIRNGTLNSRITDPSLRDCI+KILKPDA+LADFVSDECSKE+WEGIVTRI
Sbjct: 241 LQLNWHDLATDIRNGTLNSRITDPSLRDCIQKILKPDAQLADFVSDECSKEEWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLD+YSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDFYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGNDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLP E NSG+DS PKLVDLVDVE+GKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPLEQNSGSDSSPKLVDLVDVEMGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEWKASVVEYTSYADTKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVE ASE+LK+WKASVVEYTSYA+TKTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVEKASEVLKQWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELMVKEAAEE-GKWPSKEVMEHCCLAMEESLNSVYRQGRVADHSIGALEIRVV 540
           GHYVIYWELMVKE  EE GKW  KEVME CCL MEES+NSVYRQGRVAD+SIGALEIRVV
Sbjct: 481 GHYVIYWELMVKEGGEEAGKWGRKEVMEECCLRMEESMNSVYRQGRVADNSIGALEIRVV 540

Query: 541 KSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTP 595
           KSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVS HFSPALPHWTP
Sbjct: 541 KSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSTHFSPALPHWTP 595

BLAST of Clc05G13000.1 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 966.1 bits (2496), Expect = 1.3e-281
Identity = 477/599 (79.63%), Postives = 527/599 (87.98%), Query Frame = 0

Query: 1   MAVHSSLSSPLGPPACENDAKPLQFIDQMTRNADAVQHAVLAEILSRNASTEYLRRYSLD 60
           MAV S+LSSPLGPPACE DAK L+FI++MTRNAD VQ  +LAEIL+RNA TEYLRR++L 
Sbjct: 1   MAVDSNLSSPLGPPACEKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLC 60

Query: 61  GATDPHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATD  TFK K+PVITYEDLQPEIQRIA GDRSPILS+HPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDRDTFKTKIPVITYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKG+YFLFVKSET+TPGGL ARPVLTSYYKS+
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSE 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240
           HF++RPYDP+ VYTSPNEAILCPDSFQSMYTQMLCGLL R  VLR+GAVFASGLLRAIRF
Sbjct: 181 HFRSRPYDPYNVYTSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRF 240

Query: 241 LQLNWQDLATDIRNGTLNSRITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300
           LQL+W   A DI  G L+S ITDPS+R C+  ILKPD  LA+F+  EC  ++WE I+TRI
Sbjct: 241 LQLHWSRFAHDIELGCLDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WP TKYLDVIVTGAMAQYIPTL+YYSGGLP+ACTMYASSECYFGLNLNPM KPSEVSYTI
Sbjct: 301 WPNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGNDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEF+P           K V+LVDV +GKEYELV+TTYAGL RYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFIPL-------GGTKAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELL-KEWKASVVEYTSYADTKTI 480
           HN+APQFHFVRRKNV+LSIDSDKTDE+ELQKAVENAS +L +E  + V EYTSYADT TI
Sbjct: 421 HNSAPQFHFVRRKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTI 480

Query: 481 PGHYVIYWELMVKEAAEEGKWPSKEVMEHCCLAMEESLNSVYRQGRVADHSIGALEIRVV 540
           PGHYV+YWEL+V++ A +   PS E +  CCL MEESLNSVYRQ RVAD+S+G LEIRVV
Sbjct: 481 PGHYVLYWELLVRDGARQ---PSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVV 540

Query: 541 KSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARTR 599
           ++GTFEELMDYAISRGASINQYKVPRCVNFTPI+ELLDSRVVSAHFSP+LPHWTP R R
Sbjct: 541 RNGTFEELMDYAISRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPERRR 589

BLAST of Clc05G13000.1 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 919.1 bits (2374), Expect = 1.9e-267
Identity = 458/605 (75.70%), Postives = 519/605 (85.79%), Query Frame = 0

Query: 1   MAVHSSLSSPL-GPPACENDAKPLQFIDQMTRNADAVQHAVLAEILSRNASTEYLRRYSL 60
           MAV S L S +      E D K L+FI++MTRN D+VQ  VL EIL+RN++TEYL+R+ L
Sbjct: 1   MAVDSPLQSRMVSATTSEKDVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDL 60

Query: 61  DGATDPHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMP 120
           DG  D  TFK+K+PV+TYEDL+PEIQRI++GD SPILSSHPI+EFLTSSGTSAGERKLMP
Sbjct: 61  DGVVDRKTFKSKVPVVTYEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMP 120

Query: 121 TIKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKS 180
           TI+E+LDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSE++T GGL ARPVLTSYYKS
Sbjct: 121 TIEEDLDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKS 180

Query: 181 DHFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIR 240
           DHFK RPYDP+ VYTSPNEAILC DS QSMY QMLCGLL RH+VLRLGAVFASGLLRAI 
Sbjct: 181 DHFKRRPYDPYNVYTSPNEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAIS 240

Query: 241 FLQLNWQDLATDIRNGTLNSRITDPSLRDCIEKIL-KPDAELADFVSDECSKEDWEGIVT 300
           FLQ NW++LA DI  GTL+SRI DP++++ + KIL KPD ELA+F+   CS+E+WEGI+T
Sbjct: 241 FLQNNWKELARDISTGTLSSRIFDPAIKNRMSKILTKPDQELAEFLVGVCSQENWEGIIT 300

Query: 301 RIWPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSY 360
           +IWP TKYLDVIVTGAMAQYIPTL+YYSGGLP+ACTMYASSE YFG+NL PMCKPSEVSY
Sbjct: 301 KIWPNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSY 360

Query: 361 TIMPNMAYFEFLPHEPNSGNDS-----PPKLVDLVDVEVGKEYELVITTYAGLYRYRVGD 420
           TIMPNMAYFEFLPH  +    +        LV+L +VEVGKEYELVITTYAGLYRYRVGD
Sbjct: 361 TIMPNMAYFEFLPHNHDGDGAAEASLDETSLVELANVEVGKEYELVITTYAGLYRYRVGD 420

Query: 421 ILRVTGFHNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEWKASVVEYTSY 480
           ILRVTGFHN+APQF F+RRKNV+LS++SDKTDEAELQKAVENAS L  E    V+EYTSY
Sbjct: 421 ILRVTGFHNSAPQFKFIRRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTSY 480

Query: 481 ADTKTIPGHYVIYWELMVKEAAEEGKWPSKEVMEHCCLAMEESLNSVYRQGRVADHSIGA 540
           A+TKTIPGHYVIYWEL+ ++  +     S+EVM  CCL MEESLNSVYRQ RVAD SIG 
Sbjct: 481 AETKTIPGHYVIYWELLGRD--QSNALMSEEVMAKCCLEMEESLNSVYRQSRVADKSIGP 540

Query: 541 LEIRVVKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWT 599
           LEIRVV++GTFEELMDYAISRGASINQYKVPRCV+FTPIMELLDSRVVSAHFSP+LPHW+
Sbjct: 541 LEIRVVRNGTFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPSLPHWS 600

BLAST of Clc05G13000.1 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 906.7 bits (2342), Expect = 9.5e-264
Identity = 454/600 (75.67%), Postives = 512/600 (85.33%), Query Frame = 0

Query: 1   MAVHSSLSSPLGPPACENDAKPLQFIDQMTRNADAVQHAVLAEILSRNASTEYLRRYSLD 60
           M V S+L SP+       D K L+FI++MTRN D VQ  V+ EILSRN+ TEYL+R+ L 
Sbjct: 1   MTVDSALRSPMMHSPSTKDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLK 60

Query: 61  GATDPHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           G TD  TFK K+PV+ Y+DL+PEIQRIA+GDRS ILSS+PI+EFLTSSGTSAGERKLMPT
Sbjct: 61  GFTDRKTFKTKVPVVIYDDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           I E++DRRQLLYSLLMPVMNLYVPGLDKGK LYFLFVK+E++TPGGL ARPVLTSYYKS+
Sbjct: 121 IDEDMDRRQLLYSLLMPVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSE 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240
            FK RP DP+ VYTSPNEAILCPDS QSMYTQMLCGLL RH+VLRLGAVFASGLLRAI F
Sbjct: 181 QFKRRPNDPYNVYTSPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGF 240

Query: 241 LQLNWQDLATDIRNGTLNSRITDPSLRDCIEKIL-KPDAELADFVSDECSKED-WEGIVT 300
           LQ NW++LA DI  GTL+SRI+DP++++ + KIL KPD ELADF++  C +++ WEGI+T
Sbjct: 241 LQTNWKELADDISTGTLSSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIIT 300

Query: 301 RIWPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSY 360
           +IWP TKYLDVIVTGAMAQYIP L+YYSGGLP+ACTMYASSE YFG+NL PMCKPSEVSY
Sbjct: 301 KIWPNTKYLDVIVTGAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSY 360

Query: 361 TIMPNMAYFEFLPHEPNSGNDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVT 420
           TIMPNMAYFEFLPH       S  +LV+L DVEVGKEYELVITTYAGL RYRVGDIL+VT
Sbjct: 361 TIMPNMAYFEFLPHHEVPTEKS--ELVELADVEVGKEYELVITTYAGLNRYRVGDILQVT 420

Query: 421 GFHNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEWKASVVEYTSYADTKT 480
           GF+N+APQF FVRRKNV+LSI+SDKTDEAELQ AVENAS LL E    V+EYTSYA+TKT
Sbjct: 421 GFYNSAPQFKFVRRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKT 480

Query: 481 IPGHYVIYWELMVKEAAEEGKWPSKEVMEHCCLAMEESLNSVYRQGRVADHSIGALEIRV 540
           IPGHYVIYWEL+VK+       P+ EVM  CCL MEESLNSVYRQ RVAD SIG LEIRV
Sbjct: 481 IPGHYVIYWELLVKDQTNP---PNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRV 540

Query: 541 VKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARTR 599
           VK+GTFEELMDYAISRGASINQYKVPRCV+FTPIMELLDSRVVS HFSPALPHW+P R R
Sbjct: 541 VKNGTFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHWSPERRR 595

BLAST of Clc05G13000.1 vs. TAIR 10
Match: AT1G59500.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 858.2 bits (2216), Expect = 3.9e-249
Identity = 430/599 (71.79%), Postives = 503/599 (83.97%), Query Frame = 0

Query: 1   MAVHSSLSSPL-GPPACENDAKPLQFIDQMTRNADAVQHAVLAEILSRNASTEYLRRYSL 60
           MAV S L S +  P   E + K L+FI+++TRN D+VQ  VL EILSRN++TEYL+R+ L
Sbjct: 1   MAVDSLLQSGMASPTTSETEVKALKFIEEITRNPDSVQEKVLGEILSRNSNTEYLKRFDL 60

Query: 61  DGATDPHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMP 120
           +GA D  +FK+K+PV+ YEDL+ +IQRI++GDRSPILSSHPI+EFLTSSGTSAGERKLMP
Sbjct: 61  NGAVDRKSFKSKVPVVIYEDLKTDIQRISNGDRSPILSSHPITEFLTSSGTSAGERKLMP 120

Query: 121 TIKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKS 180
           TI+E+++RRQLL +LLMPVMNLYVPGLDKGKGLYFLFVKSE+ T GGL ARP LTSYYKS
Sbjct: 121 TIEEDINRRQLLGNLLMPVMNLYVPGLDKGKGLYFLFVKSESTTSGGLPARPALTSYYKS 180

Query: 181 DHFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIR 240
           D+F+T   D   VYTSP EAILC DS QSMYTQMLCGLL RH+V RLGAVF SGLLRAI 
Sbjct: 181 DYFRTSDSD--SVYTSPKEAILCCDSSQSMYTQMLCGLLMRHEVNRLGAVFPSGLLRAIS 240

Query: 241 FLQLNWQDLATDIRNGTLNSRITDPSLRDCIEKIL-KPDAELADFVSDECSKEDWEGIVT 300
           FLQ NW++L+ DI  GTL+S+I D +++  +  IL KPD ELA+F+   CS+E+WEGI+T
Sbjct: 241 FLQNNWKELSQDISTGTLSSKIFDHAIKTRMSNILNKPDQELAEFLIGVCSQENWEGIIT 300

Query: 301 RIWPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSY 360
           +IWP TKYLDVIVTGAMA+YIP L+YYSGGLP+A  +YASSE YFG+NLNPMCKPSEVSY
Sbjct: 301 KIWPNTKYLDVIVTGAMAEYIPMLEYYSGGLPMASMIYASSESYFGINLNPMCKPSEVSY 360

Query: 361 TIMPNMAYFEFLPH-EPNSGNDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRV 420
           TI PNMAYFEFLPH     G      LV+L DVEVGKEYELVITTYAGLYRYRVGDILRV
Sbjct: 361 TIFPNMAYFEFLPHNHDGDGGVEATSLVELADVEVGKEYELVITTYAGLYRYRVGDILRV 420

Query: 421 TGFHNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEWKASVVEYTSYADTK 480
           TGFHN+APQF F+RR+NV+LSI+SDKTDEA+LQKAVENAS LL E    V+EYTSYADTK
Sbjct: 421 TGFHNSAPQFKFIRRENVLLSIESDKTDEADLQKAVENASRLLAEQGTRVIEYTSYADTK 480

Query: 481 TIPGHYVIYWELMVKEAAEEGKWPSKEVMEHCCLAMEESLNSVYRQGRVADHSIGALEIR 540
           TIPGHYVIYWEL+ ++  +    PS EVM  CCL MEESLN+VYRQ RV+D SIG LEIR
Sbjct: 481 TIPGHYVIYWELLSRD--QSNALPSDEVMAKCCLEMEESLNAVYRQSRVSDKSIGPLEIR 540

Query: 541 VVKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPAR 597
           VV++GTFEELMD++ISRG+SINQYKVPRCV+ TPIM+LLDSRVVSAHFSP+LPHW+P R
Sbjct: 541 VVQNGTFEELMDFSISRGSSINQYKVPRCVSLTPIMKLLDSRVVSAHFSPSLPHWSPER 595

BLAST of Clc05G13000.1 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 824.3 bits (2128), Expect = 6.2e-239
Identity = 406/591 (68.70%), Postives = 478/591 (80.88%), Query Frame = 0

Query: 17  ENDAKPLQFIDQMTRNADAVQHAVLAEILSRNASTEYLRRYSLDGATDPHTFKAKLPVIT 76
           E +   LQFI+ +T NAD VQ  VL EILSRNA  EYL+R+ L+G TD  TFK  +PV+T
Sbjct: 19  EKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPVVT 78

Query: 77  YEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLM 136
           YED+QPEI RIA+GD+S +L S+PISEFLTSSGTS GERKLMPTI+EELDRR LLYSLLM
Sbjct: 79  YEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLM 138

Query: 137 PVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSDHFKTRPYDPFMVYTSP 196
           PVM+ +VPGLDKGKG+YFLF+KSE++TPGGL ARPVLTSYYKS HFK RPYDP+  YTSP
Sbjct: 139 PVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTSP 198

Query: 197 NEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRFLQLNWQDLATDIRNGT 256
           N+ ILC DS+QSMY+QMLCGL Q  +VLR+GAVFASG +RAI+FL+ +W +LA DIR GT
Sbjct: 199 NQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTGT 258

Query: 257 LNSRITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRIWPKTKYLDVIVTGAMA 316
           L+S ITD S+R+ + +ILKPD +LADFV  EC K  W+GI+TR+WP TKY+DVIVTG M+
Sbjct: 259 LSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGTMS 318

Query: 317 QYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTIMPNMAYFEFLPHEPNS 376
           QYIPTLDYYS GLPL CTMYASSECYFG+NL P+CKPSEVSYT++PNMAYFEFLP   NS
Sbjct: 319 QYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHRNS 378

Query: 377 G---NDSPPK---------LVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNAA 436
           G   + S PK         LVDLVDV++G+EYELV+TTYAGLYRYRVGD+L V GF N A
Sbjct: 379 GVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFKNNA 438

Query: 437 PQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEWKASVVEYTSYADTKTIPGHYV 496
           PQF F+ RKNVVLSIDSDKTDE ELQ AV+NA   L  + AS+ EYTSYADT +IPGHYV
Sbjct: 439 PQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYV 498

Query: 497 IYWELMVKEAAEEGKWP-SKEVMEHCCLAMEESLNSVYRQGRVADHSIGALEIRVVKSGT 556
           ++WEL +      G  P    V E CCL +EESLNSVYRQGRV+D SIG LEI++V+SGT
Sbjct: 499 LFWELCL-----NGNTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGT 558

Query: 557 FEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTP 595
           F++LMDYAIS GASINQYK PRCV F PI+ELL+SRVV ++FSP  P W+P
Sbjct: 559 FDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSP 604

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038877583.10.0e+0098.49probable indole-3-acetic acid-amido synthetase GH3.1 [Benincasa hispida][more]
XP_008465614.10.0e+0095.83PREDICTED: probable indole-3-acetic acid-amido synthetase GH3.1 [Cucumis melo] >... [more]
XP_022922958.10.0e+0095.82probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita moschata][more]
KAG6576928.10.0e+0095.65putative indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma ... [more]
XP_022985331.10.0e+0095.32probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
O823331.9e-28079.63Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=... [more]
O221901.3e-26275.67Indole-3-acetic acid-amido synthetase GH3.3 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
A3BLS01.8e-25975.04Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=399... [more]
Q0D4Z61.8e-25975.04Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japonica OX=3... [more]
P0C0M27.1e-25673.27Probable indole-3-acetic acid-amido synthetase GH3.2 OS=Oryza sativa subsp. japo... [more]
Match NameE-valueIdentityDescription
A0A5A7T1480.0e+0095.83Putative indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo var. makuwa... [more]
A0A1S3CP720.0e+0095.83probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo OX=3656 GN=... [more]
A0A6J1E8A00.0e+0095.82probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita moschata OX=36... [more]
A0A6J1J7V10.0e+0095.32probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita maxima OX=3661... [more]
A0A0A0L6980.0e+0094.45Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G198490 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G14960.11.3e-28179.63Auxin-responsive GH3 family protein [more]
AT4G37390.11.9e-26775.70Auxin-responsive GH3 family protein [more]
AT2G23170.19.5e-26475.67Auxin-responsive GH3 family protein [more]
AT1G59500.13.9e-24971.79Auxin-responsive GH3 family protein [more]
AT5G54510.16.2e-23968.70Auxin-responsive GH3 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 446..466
NoneNo IPR availablePANTHERPTHR31901:SF7INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE GH3.2-RELATEDcoord: 5..596
IPR004993GH3 familyPFAMPF03321GH3coord: 26..569
e-value: 1.6E-205
score: 684.0
IPR004993GH3 familyPANTHERPTHR31901GH3 DOMAIN-CONTAINING PROTEINcoord: 5..596

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Clc05G13000Clc05G13000gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Clc05G13000.1-exonClc05G13000.1-exon-ClcChr05:10988964..10989474exon
Clc05G13000.1-exonClc05G13000.1-exon-ClcChr05:10989567..10989668exon
Clc05G13000.1-exonClc05G13000.1-exon-ClcChr05:10989790..10991213exon


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Clc05G13000.1-five_prime_utrClc05G13000.1-five_prime_utr-ClcChr05:10988964..10989154five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Clc05G13000.1-cdsClc05G13000.1-cds-ClcChr05:10989155..10989474CDS
Clc05G13000.1-cdsClc05G13000.1-cds-ClcChr05:10989567..10989668CDS
Clc05G13000.1-cdsClc05G13000.1-cds-ClcChr05:10989790..10991164CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Clc05G13000.1-three_prime_utrClc05G13000.1-three_prime_utr-ClcChr05:10991165..10991213three_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Clc05G13000.1Clc05G13000.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016881 acid-amino acid ligase activity