Cla97C11G207850.2 (mRNA) Watermelon (97103) v2.5

Overview


Name	Cla97C11G207850.2
Type	mRNA
Organism	Citrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
Description	Mediator of RNA polymerase II transcription subunit 20
Location	Cla97Chr11: 1646999 .. 1649324 (+)
Sequence length	1009
RNA-Seq Expression	Cla97C11G207850.2
Synteny	Cla97C11G207850.2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAAAAATCAAAAGAATTGTATGGATCCCCAAAGGCCATGGCAAGAAGTACTCAAGTTTGGGCTGGCAGTGGTCTTTATACTTTCCATTGGGCTTTCCGCAAAACTTGCAAATTGTTCCTAACCTGCTCCGGATCCGACTAATCTCCGCCGTCTCGCTGCAGCTTTAGTCCGGCCGGAAGTATGCCGCTAAAATGGTAAAACCCCACAAACCCCGATCGAAAAACTTCTCATCCCTTTTCTCTTGGTTTTGACTCTTGATCTACCTTCATCGTTTTCCTTTGCTTAGGGTTTTGCATTGGCAACCCAATGCCGGCAGCACTGTTAACAGTCAGATACTCAACGAGGTTTCACAATGTGTTGAGAGTATCCATGGCGTTAAAGGAGGAAAATGGAAAGCTACTCTCACTTTCTACAAACCCATCTTACGAGGTAATCGCCCTGTTTTTCTTCAGACGTTTTTCGGAATTTGATTACCGAAATGAATCTTAAATCTGTCAACAGACCAAAACCTACCGGCGGCCGAGTTCCCGCGGGATTTTCTCGGAATTTCGCTGCCCGAGCAACCAAACAAGTATTACCTTATAATTCGTGGCCAACGGATTGTCTTGGAAGCTGATTTCACGATTCAGGCCATAATGGAGAAGTTGCAGTCTTACAAATTGCGCGTTGCTCTCAATTTCGAGGTTGACCACTCAACTTCTCTAGTTCTGATGTACTCTGCATCTATACCATGTCCTGAGATTCGAATTTGAAGTTGATTCTTGTGGCTTTCTAGTCTCTGCTCTTTTGCCCTTTTTGTTGATAAATCGTTGATTACAGGCAGTTCACATTATTGGTTTCTCAGGGGTTCCAGTATCAGTTAGGGGATTTCCAACTGAGAGTGGGAAAAGTTGTTCCCAACAATTCCGAGAATTTGAGAGGAATAGTTATGGAGGTAGGGCTCATTTCATCATTTCATGTCAACTCTGTTTTTGTGTTATTGCATGCTCTTCTGGTTGTTTGTTTTGTTGTTTTTTCATGTATTTTTCCTTATCTGAGCGAGCAGTTTCTTTTCCCTTGGGCTGTTTCAACACAATGTTTCGCCTCTGTCTTCTCATCTTCAAAACAATGTAAATTTTAACACAATCTATAAGTTTAAGCTTTTGGGTTCAAAGTAGAATGTCATTTCCTCCTTCAATTAAAATAGATTTTCACTTGTTGGGACTTGTATAACTTTTCAACCCTACTAGTGAAGAGGATGGTTGAAGTGTTGATAAGTTGCTTGGTTCATTAGTGATTTAAAACAATAGAGTAGAAGAAAAAAGAAATCATTCATCATAATTCTCTGAAAAACTTATCAGTAAGCTTTGGAGTCAGGGATCTTCTGTCAAGAAGTAGTGAGATCTGATTAGGATATCTTTGCAAAAGCCTGTGTAGGATAAGTTATTGAAGTCGTTAATGTGATCTTCACATTTTTTTTTATATTTTATACTCATTGTTGCACACGAATCAAAGTGTTTTGAGTAAATTCCTCCGAAGGAGTTATTTAGTTTGGAAAATGTCTTCCAGTTCACTTGAATGCTTGATGAGAACGTGAATGTGTCTGTCTTTGCTTAATCCTGAAACAGGTAAAGTAAAGAAAGATGCCGATTTCAAATGTCTGTCCTTCATTATCATTTTTTCTTGAAAAAACGCATTATACCATGTTCTCTATTGTATTGTCAAGTCCAAGTAGTAAATTCAATCGCAATTGCTTGCACTTCTCTACCAAATCTTGATTCAGTTTTGTAGTTTGTTTTTAATTAGTACGATGAACATCATATTGGAAGTACGGTTAAATCAAGTGGGATTTAATTAGAAGCTAGAATCTCTCTGTCAAATGTGGCCATGTTCTATTTTTTGGGTGTATATATGAAAATTGCTCTTTGTTGCTGTTCCAATGAAATATTTTGGACACTTGATAGAGATTCAATAGTGTATTAAAAAACCTGCATATGGCAGATTGAGTATCTCCCAATTTCTTCAATGGAGAAATCAAGACAGGTAATGGAGGAGTTCTTCGATGTATGGCAAGAAGCCATGTCAAAAAGTTCATTACCAGGTCGTTTCATGCACATAGAACCAAACTTTGCAGAATATGGACTGTCGGATTACTACACTTCTCAACACACAGCAGTTCAGTATGCCAACGTGATGGCTCAACTTATCGCTACTGTGCAAGCAGTTCAAGCAAGAAATTAGCATCAAAATTGCTTTTATTTATCAGGTTGAGATCTGTACTGATGACCCATTCTCAACTCCCTAATGACTTGAATTAAAAAACAAATGAAATATTTCATATTCTGC

mRNA sequence

CAAAAAATCAAAAGAATTGTATGGATCCCCAAAGGCCATGGCAAGAAGTACTCAAGTTTGGGCTGGCAGTGGTCTTTATACTTTCCATTGGGCTTTCCGCAAAACTTGCAAATTGTTCCTAACCTGCTCCGGATCCGACTAATCTCCGCCGTCTCGCTGCAGCTTTAGTCCGGCCGGAAGTATGCCGCTAAAATGGGTTTTGCATTGGCAACCCAATGCCGGCAGCACTGTTAACAGTCAGATACTCAACGAGGTTTCACAATGTGTTGAGAGTATCCATGGCGTTAAAGGAGGAAAATGGAAAGCTACTCTCACTTTCTACAAACCCATCTTACGAGACCAAAACCTACCGGCGGCCGAGTTCCCGCGGGATTTTCTCGGAATTTCGCTGCCCGAGCAACCAAACAAGTATTACCTTATAATTCGTGGCCAACGGATTGTCTTGGAAGCTGATTTCACGATTCAGGCCATAATGGAGAAGTTGCAGTCTTACAAATTGCGCGTTGCTCTCAATTTCGAGGTTGACCACTCAACTTCTCTAGTTCTGATGCAGTTCACATTATTGGTTTCTCAGGGGTTCCAGTATCAGTTAGGGGATTTCCAACTGAGAGTGGGAAAAGTTGTTCCCAACAATTCCGAGAATTTGAGAGGAATAGTTATGGAGATTGAGTATCTCCCAATTTCTTCAATGGAGAAATCAAGACAGGTAATGGAGGAGTTCTTCGATGTATGGCAAGAAGCCATGTCAAAAAGTTCATTACCAGGTCGTTTCATGCACATAGAACCAAACTTTGCAGAATATGGACTGTCGGATTACTACACTTCTCAACACACAGCAGTTCAGTATGCCAACGTGATGGCTCAACTTATCGCTACTGTGCAAGCAGTTCAAGCAAGAAATTAGCATCAAAATTGCTTTTATTTATCAGGTTGAGATCTGTACTGATGACCCATTCTCAACTCCCTAATGACTTGAATTAAAAAACAAATGAAATATTTCATATTCTGC

Coding sequence (CDS)

ATGCCGCTAAAATGGGTTTTGCATTGGCAACCCAATGCCGGCAGCACTGTTAACAGTCAGATACTCAACGAGGTTTCACAATGTGTTGAGAGTATCCATGGCGTTAAAGGAGGAAAATGGAAAGCTACTCTCACTTTCTACAAACCCATCTTACGAGACCAAAACCTACCGGCGGCCGAGTTCCCGCGGGATTTTCTCGGAATTTCGCTGCCCGAGCAACCAAACAAGTATTACCTTATAATTCGTGGCCAACGGATTGTCTTGGAAGCTGATTTCACGATTCAGGCCATAATGGAGAAGTTGCAGTCTTACAAATTGCGCGTTGCTCTCAATTTCGAGGTTGACCACTCAACTTCTCTAGTTCTGATGCAGTTCACATTATTGGTTTCTCAGGGGTTCCAGTATCAGTTAGGGGATTTCCAACTGAGAGTGGGAAAAGTTGTTCCCAACAATTCCGAGAATTTGAGAGGAATAGTTATGGAGATTGAGTATCTCCCAATTTCTTCAATGGAGAAATCAAGACAGGTAATGGAGGAGTTCTTCGATGTATGGCAAGAAGCCATGTCAAAAAGTTCATTACCAGGTCGTTTCATGCACATAGAACCAAACTTTGCAGAATATGGACTGTCGGATTACTACACTTCTCAACACACAGCAGTTCAGTATGCCAACGTGATGGCTCAACTTATCGCTACTGTGCAAGCAGTTCAAGCAAGAAATTAG

Protein sequence

MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAEFPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEVDHSTSLVLMQFTLLVSQGFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQVMEEFFDVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQARN

Homology

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BLAST of Cla97C11G207850.2 vs. NCBI nr
Match: XP_038889134.1 (mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Benincasa hispida])

HSP 1 Score: 429.9 bits (1104), Expect = 1.5e-116
Identity = 220/241 (91.29%), Postives = 222/241 (92.12%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE
Sbjct: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEVDHSTSL 120
           FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFE       
Sbjct: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFE------- 120

Query: 121 VLMQFTLLVSQGFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQVMEEF 180
                      GFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSR+VMEEF
Sbjct: 121 -----------GFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRKVMEEF 180

Query: 181 FDVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQ-AR 240
           F+VWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQ AR
Sbjct: 181 FEVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSAR 223

[Close]

BLAST of Cla97C11G207850.2 vs. NCBI nr
Match: XP_004141069.1 (mediator of RNA polymerase II transcription subunit 20a [Cucumis sativus] >KGN60509.1 hypothetical protein Csa_019466 [Cucumis sativus])

HSP 1 Score: 426.0 bits (1094), Expect = 2.1e-115
Identity = 219/241 (90.87%), Postives = 219/241 (90.87%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQN PAAE
Sbjct: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNSPAAE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEVDHSTSL 120
           FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFE       
Sbjct: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFE------- 120

Query: 121 VLMQFTLLVSQGFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQVMEEF 180
                      GFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQVMEEF
Sbjct: 121 -----------GFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQVMEEF 180

Query: 181 FDVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQ-AR 240
           FDVWQEAMSKSSLPGRFMHIE NFAEYGLSDYYTSQHTAVQY NVMAQLIATVQAVQ AR
Sbjct: 181 FDVWQEAMSKSSLPGRFMHIESNFAEYGLSDYYTSQHTAVQYGNVMAQLIATVQAVQSAR 223

[Close]

BLAST of Cla97C11G207850.2 vs. NCBI nr
Match: XP_038889135.1 (mediator of RNA polymerase II transcription subunit 20a-like isoform X2 [Benincasa hispida])

HSP 1 Score: 422.2 bits (1084), Expect = 3.1e-114
Identity = 217/241 (90.04%), Postives = 219/241 (90.87%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE
Sbjct: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEVDHSTSL 120
           FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFE       
Sbjct: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFE------- 120

Query: 121 VLMQFTLLVSQGFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQVMEEF 180
                         YQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSR+VMEEF
Sbjct: 121 --------------YQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRKVMEEF 180

Query: 181 FDVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQ-AR 240
           F+VWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQ AR
Sbjct: 181 FEVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSAR 220

[Close]

BLAST of Cla97C11G207850.2 vs. NCBI nr
Match: XP_022946745.1 (mediator of RNA polymerase II transcription subunit 20a-like [Cucurbita moschata] >XP_022999230.1 mediator of RNA polymerase II transcription subunit 20a-like [Cucurbita maxima] >XP_023546929.1 mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023546930.1 mediator of RNA polymerase II transcription subunit 20a-like isoform X2 [Cucurbita pepo subsp. pepo] >KAG6599532.1 Mediator of RNA polymerase II transcription subunit 20a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 421.4 bits (1082), Expect = 5.2e-114
Identity = 215/241 (89.21%), Postives = 219/241 (90.87%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRD N+ A E
Sbjct: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEVDHSTSL 120
           FPRDFLGIS+PEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFE       
Sbjct: 61  FPRDFLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFE------- 120

Query: 121 VLMQFTLLVSQGFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQVMEEF 180
                      GFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKS+QVMEEF
Sbjct: 121 -----------GFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEF 180

Query: 181 FDVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQ-AR 240
           F+VWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQ AR
Sbjct: 181 FEVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSAR 223

[Close]

BLAST of Cla97C11G207850.2 vs. NCBI nr
Match: XP_008459711.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Cucumis melo])

HSP 1 Score: 421.0 bits (1081), Expect = 6.8e-114
Identity = 215/241 (89.21%), Postives = 218/241 (90.46%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQN PAAE
Sbjct: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNSPAAE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEVDHSTSL 120
           FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFE       
Sbjct: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFE------- 120

Query: 121 VLMQFTLLVSQGFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQVMEEF 180
                      GFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQ+M+EF
Sbjct: 121 -----------GFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQIMDEF 180

Query: 181 FDVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQ-AR 240
           FDVWQEAMSKSSLPGRFMHIE NFAEYGLSD+YT QHTAVQY NVMAQLIATVQAVQ AR
Sbjct: 181 FDVWQEAMSKSSLPGRFMHIESNFAEYGLSDFYTPQHTAVQYGNVMAQLIATVQAVQSAR 223

[Close]

BLAST of Cla97C11G207850.2 vs. ExPASy Swiss-Prot
Match: Q6NPF4 (Mediator of RNA polymerase II transcription subunit 20a OS=Arabidopsis thaliana OX=3702 GN=MED20A PE=1 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 9.7e-87
Identity = 161/237 (67.93%), Postives = 192/237 (81.01%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE 60
           MP+KWVLHWQPN GSTV+SQILNE +QCVESI+GVK G+WKATL +YKP+L+DQ     E
Sbjct: 1   MPVKWVLHWQPNQGSTVSSQILNEATQCVESINGVKEGRWKATLNYYKPMLKDQ-ANQLE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEVDHSTSL 120
           FPRDFLGISL +QPNKYY IIR QRIVLEAD +IQ IMEKLQSYK +VAL F+       
Sbjct: 61  FPRDFLGISLADQPNKYYFIIRTQRIVLEADSSIQLIMEKLQSYKSKVALYFD------- 120

Query: 121 VLMQFTLLVSQGFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQVMEEF 180
                      GFQYQLGDF+LRVGKVVP +SEN+RGIVME+EYLPISSMEK+++VMEEF
Sbjct: 121 -----------GFQYQLGDFRLRVGKVVPTHSENVRGIVMEVEYLPISSMEKAQKVMEEF 180

Query: 181 FDVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQ 238
            ++W EA++K SLPG+F++I+ NF E+GL D YT QHTAV+YA VMA +IATVQAV+
Sbjct: 181 LEIWNEALAKRSLPGKFVNIDLNFGEFGLGDIYTPQHTAVRYALVMAHMIATVQAVR 218

[Close]

BLAST of Cla97C11G207850.2 vs. ExPASy Swiss-Prot
Match: Q9M0S0 (Mediator of RNA polymerase II transcription subunit 20b OS=Arabidopsis thaliana OX=3702 GN=MED20B PE=3 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 5.3e-77
Identity = 141/235 (60.00%), Postives = 181/235 (77.02%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE 60
           MP+KW+LHWQPN GST +SQILNEV+Q +ES++GVK G WKATL +YKP+L+DQ    AE
Sbjct: 1   MPVKWLLHWQPNQGSTFSSQILNEVTQSIESLNGVKEGTWKATLNYYKPMLQDQ-ANQAE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEVDHSTSL 120
           FPR+F+GISLPE+P+KYY +IR QRIV+EAD +IQ IME LQSYK +++  FE       
Sbjct: 61  FPREFVGISLPEEPDKYYFVIRSQRIVVEADSSIQMIMESLQSYKCKLSFYFE------- 120

Query: 121 VLMQFTLLVSQGFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQVMEEF 180
                      G +YQLGDF+LRVGKVVP ++E +RG+VME+EYLPISSM  ++++MEEF
Sbjct: 121 -----------GLEYQLGDFRLRVGKVVPTHAETIRGVVMEVEYLPISSMGMAKKLMEEF 180

Query: 181 FDVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQA 236
            ++WQEAMSK SLPG+F++ E NF ++GL D YT QHTAV YA  MA L+A +QA
Sbjct: 181 LEIWQEAMSKRSLPGKFVNKELNFEKFGLGDNYTPQHTAVGYAFFMANLMAAIQA 216

[Close]

BLAST of Cla97C11G207850.2 vs. ExPASy TrEMBL
Match: A0A0A0LHQ1 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis sativus OX=3659 GN=MED20 PE=3 SV=1)

HSP 1 Score: 426.0 bits (1094), Expect = 1.0e-115
Identity = 219/241 (90.87%), Postives = 219/241 (90.87%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQN PAAE
Sbjct: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNSPAAE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEVDHSTSL 120
           FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFE       
Sbjct: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFE------- 120

Query: 121 VLMQFTLLVSQGFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQVMEEF 180
                      GFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQVMEEF
Sbjct: 121 -----------GFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQVMEEF 180

Query: 181 FDVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQ-AR 240
           FDVWQEAMSKSSLPGRFMHIE NFAEYGLSDYYTSQHTAVQY NVMAQLIATVQAVQ AR
Sbjct: 181 FDVWQEAMSKSSLPGRFMHIESNFAEYGLSDYYTSQHTAVQYGNVMAQLIATVQAVQSAR 223

[Close]

BLAST of Cla97C11G207850.2 vs. ExPASy TrEMBL
Match: A0A6J1KEU1 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucurbita maxima OX=3661 GN=LOC111493666 PE=3 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 2.5e-114
Identity = 215/241 (89.21%), Postives = 219/241 (90.87%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRD N+ A E
Sbjct: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEVDHSTSL 120
           FPRDFLGIS+PEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFE       
Sbjct: 61  FPRDFLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFE------- 120

Query: 121 VLMQFTLLVSQGFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQVMEEF 180
                      GFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKS+QVMEEF
Sbjct: 121 -----------GFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEF 180

Query: 181 FDVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQ-AR 240
           F+VWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQ AR
Sbjct: 181 FEVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSAR 223

[Close]

BLAST of Cla97C11G207850.2 vs. ExPASy TrEMBL
Match: A0A6J1G4U3 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucurbita moschata OX=3662 GN=LOC111450720 PE=3 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 2.5e-114
Identity = 215/241 (89.21%), Postives = 219/241 (90.87%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRD N+ A E
Sbjct: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEVDHSTSL 120
           FPRDFLGIS+PEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFE       
Sbjct: 61  FPRDFLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFE------- 120

Query: 121 VLMQFTLLVSQGFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQVMEEF 180
                      GFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKS+QVMEEF
Sbjct: 121 -----------GFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEF 180

Query: 181 FDVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQ-AR 240
           F+VWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQ AR
Sbjct: 181 FEVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSAR 223

[Close]

BLAST of Cla97C11G207850.2 vs. ExPASy TrEMBL
Match: A0A5D3CH02 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis melo var. makuwa OX=1194695 GN=MED20 PE=3 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 3.3e-114
Identity = 215/241 (89.21%), Postives = 218/241 (90.46%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQN PAAE
Sbjct: 49  MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNSPAAE 108

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEVDHSTSL 120
           FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFE       
Sbjct: 109 FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFE------- 168

Query: 121 VLMQFTLLVSQGFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQVMEEF 180
                      GFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQ+M+EF
Sbjct: 169 -----------GFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQIMDEF 228

Query: 181 FDVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQ-AR 240
           FDVWQEAMSKSSLPGRFMHIE NFAEYGLSD+YT QHTAVQY NVMAQLIATVQAVQ AR
Sbjct: 229 FDVWQEAMSKSSLPGRFMHIESNFAEYGLSDFYTPQHTAVQYGNVMAQLIATVQAVQSAR 271

[Close]

BLAST of Cla97C11G207850.2 vs. ExPASy TrEMBL
Match: A0A1S3CAW6 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis melo OX=3656 GN=LOC103498750 PE=3 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 3.3e-114
Identity = 215/241 (89.21%), Postives = 218/241 (90.46%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQN PAAE
Sbjct: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNSPAAE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEVDHSTSL 120
           FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFE       
Sbjct: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFE------- 120

Query: 121 VLMQFTLLVSQGFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQVMEEF 180
                      GFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQ+M+EF
Sbjct: 121 -----------GFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQIMDEF 180

Query: 181 FDVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQ-AR 240
           FDVWQEAMSKSSLPGRFMHIE NFAEYGLSD+YT QHTAVQY NVMAQLIATVQAVQ AR
Sbjct: 181 FDVWQEAMSKSSLPGRFMHIESNFAEYGLSDFYTPQHTAVQYGNVMAQLIATVQAVQSAR 223

[Close]

BLAST of Cla97C11G207850.2 vs. TAIR 10
Match: AT2G28230.1 (TATA-binding related factor (TRF) of subunit 20 of Mediator complex )

HSP 1 Score: 321.2 bits (822), Expect = 6.9e-88
Identity = 161/237 (67.93%), Postives = 192/237 (81.01%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE 60
           MP+KWVLHWQPN GSTV+SQILNE +QCVESI+GVK G+WKATL +YKP+L+DQ     E
Sbjct: 1   MPVKWVLHWQPNQGSTVSSQILNEATQCVESINGVKEGRWKATLNYYKPMLKDQ-ANQLE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEVDHSTSL 120
           FPRDFLGISL +QPNKYY IIR QRIVLEAD +IQ IMEKLQSYK +VAL F+       
Sbjct: 61  FPRDFLGISLADQPNKYYFIIRTQRIVLEADSSIQLIMEKLQSYKSKVALYFD------- 120

Query: 121 VLMQFTLLVSQGFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQVMEEF 180
                      GFQYQLGDF+LRVGKVVP +SEN+RGIVME+EYLPISSMEK+++VMEEF
Sbjct: 121 -----------GFQYQLGDFRLRVGKVVPTHSENVRGIVMEVEYLPISSMEKAQKVMEEF 180

Query: 181 FDVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQ 238
            ++W EA++K SLPG+F++I+ NF E+GL D YT QHTAV+YA VMA +IATVQAV+
Sbjct: 181 LEIWNEALAKRSLPGKFVNIDLNFGEFGLGDIYTPQHTAVRYALVMAHMIATVQAVR 218

[Close]

BLAST of Cla97C11G207850.2 vs. TAIR 10
Match: AT4G09070.1 (TATA-binding related factor (TRF) of subunit 20 of Mediator complex )

HSP 1 Score: 288.9 bits (738), Expect = 3.8e-78
Identity = 141/235 (60.00%), Postives = 181/235 (77.02%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE 60
           MP+KW+LHWQPN GST +SQILNEV+Q +ES++GVK G WKATL +YKP+L+DQ    AE
Sbjct: 1   MPVKWLLHWQPNQGSTFSSQILNEVTQSIESLNGVKEGTWKATLNYYKPMLQDQ-ANQAE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEVDHSTSL 120
           FPR+F+GISLPE+P+KYY +IR QRIV+EAD +IQ IME LQSYK +++  FE       
Sbjct: 61  FPREFVGISLPEEPDKYYFVIRSQRIVVEADSSIQMIMESLQSYKCKLSFYFE------- 120

Query: 121 VLMQFTLLVSQGFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQVMEEF 180
                      G +YQLGDF+LRVGKVVP ++E +RG+VME+EYLPISSM  ++++MEEF
Sbjct: 121 -----------GLEYQLGDFRLRVGKVVPTHAETIRGVVMEVEYLPISSMGMAKKLMEEF 180

Query: 181 FDVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQA 236
            ++WQEAMSK SLPG+F++ E NF ++GL D YT QHTAV YA  MA L+A +QA
Sbjct: 181 LEIWQEAMSKRSLPGKFVNKELNFEKFGLGDNYTPQHTAVGYAFFMANLMAAIQA 216

[Close]

BLAST of Cla97C11G207850.2 vs. TAIR 10
Match: AT2G28020.1 (BEST Arabidopsis thaliana protein match is: TATA-binding related factor (TRF) of subunit 20 of Mediator complex (TAIR:AT2G28230.1); Has 37 Blast hits to 37 proteins in 13 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 37; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 83.2 bits (204), Expect = 3.2e-16
Identity = 42/68 (61.76%), Postives = 55/68 (80.88%), Query Frame = 0


Query: 170 MEKSRQVMEEFFDVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQ-YANVMAQ 229
           ME +R+VMEEF ++W EA++K SLPG+F++ + NF E+GL D YT QHTAV+ YA VMA 
Sbjct: 1   MEIARKVMEEFLEIWNEALAKRSLPGKFVNKDLNFGEFGLGDNYTPQHTAVRNYALVMAH 60

Query: 230 LIATVQAV 237
           +IATVQAV
Sbjct: 61  MIATVQAV 68

The following BLAST results are available for this feature:

BLAST of Cla97C11G207850.2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038889134.1	1.5e-116	91.29	mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Beninca...	[more]
XP_004141069.1	2.1e-115	90.87	mediator of RNA polymerase II transcription subunit 20a [Cucumis sativus] >KGN60...	[more]
XP_038889135.1	3.1e-114	90.04	mediator of RNA polymerase II transcription subunit 20a-like isoform X2 [Beninca...	[more]
XP_022946745.1	5.2e-114	89.21	mediator of RNA polymerase II transcription subunit 20a-like [Cucurbita moschata...	[more]
XP_008459711.1	6.8e-114	89.21	PREDICTED: mediator of RNA polymerase II transcription subunit 20a-like isoform ...	[more]

BLAST of Cla97C11G207850.2 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NPF4	9.7e-87	67.93	Mediator of RNA polymerase II transcription subunit 20a OS=Arabidopsis thaliana ...	[more]
Q9M0S0	5.3e-77	60.00	Mediator of RNA polymerase II transcription subunit 20b OS=Arabidopsis thaliana ...	[more]

BLAST of Cla97C11G207850.2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LHQ1	1.0e-115	90.87	Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis sativus OX=365...	[more]
A0A6J1KEU1	2.5e-114	89.21	Mediator of RNA polymerase II transcription subunit 20 OS=Cucurbita maxima OX=36...	[more]
A0A6J1G4U3	2.5e-114	89.21	Mediator of RNA polymerase II transcription subunit 20 OS=Cucurbita moschata OX=...	[more]
A0A5D3CH02	3.3e-114	89.21	Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis melo var. maku...	[more]
A0A1S3CAW6	3.3e-114	89.21	Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis melo OX=3656 G...	[more]

BLAST of Cla97C11G207850.2 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G28230.1	6.9e-88	67.93	TATA-binding related factor (TRF) of subunit 20 of Mediator complex	[more]
AT4G09070.1	3.8e-78	60.00	TATA-binding related factor (TRF) of subunit 20 of Mediator complex	[more]
AT2G28020.1	3.2e-16	61.76	BEST Arabidopsis thaliana protein match is: TATA-binding related factor (TRF) of...	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013921	Mediator complex, subunit Med20	PFAM	PF08612	Med20	coord: 1..226 e-value: 1.9E-37 score: 128.8
IPR013921	Mediator complex, subunit Med20	PANTHER	PTHR12465	UBIQUITIN SPECIFIC PROTEASE HOMOLOG 49	coord: 6..235
None	No IPR available	PANTHER	PTHR12465:SF0	MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 20	coord: 6..235

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cla97C11G207850	Cla97C11G207850	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cla97C11G207850.2-exon	Cla97C11G207850.2-exon-Cla97Chr11:1646999..1647193	exon
Cla97C11G207850.2-exon	Cla97C11G207850.2-exon-Cla97Chr11:1647286..1647428	exon
Cla97C11G207850.2-exon	Cla97C11G207850.2-exon-Cla97Chr11:1647501..1647711	exon
Cla97C11G207850.2-exon	Cla97C11G207850.2-exon-Cla97Chr11:1647819..1647933	exon
Cla97C11G207850.2-exon	Cla97C11G207850.2-exon-Cla97Chr11:1648980..1649324	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cla97C11G207850.2-five_prime_utr	Cla97C11G207850.2-five_prime_utr-Cla97Chr11:1646999..1647179	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cla97C11G207850.2-cds	Cla97C11G207850.2-cds-Cla97Chr11:1647180..1647193	CDS
Cla97C11G207850.2-cds	Cla97C11G207850.2-cds-Cla97Chr11:1647286..1647428	CDS
Cla97C11G207850.2-cds	Cla97C11G207850.2-cds-Cla97Chr11:1647501..1647711	CDS
Cla97C11G207850.2-cds	Cla97C11G207850.2-cds-Cla97Chr11:1647819..1647933	CDS
Cla97C11G207850.2-cds	Cla97C11G207850.2-cds-Cla97Chr11:1648980..1649219	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cla97C11G207850.2-three_prime_utr	Cla97C11G207850.2-three_prime_utr-Cla97Chr11:1649220..1649324	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cla97C11G207850.2	Cla97C11G207850.2-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006357	regulation of transcription by RNA polymerase II
biological_process	GO:0045893	positive regulation of transcription, DNA-templated
biological_process	GO:0006383	transcription by RNA polymerase III
cellular_component	GO:0016592	mediator complex
cellular_component	GO:0005666	RNA polymerase III complex
molecular_function	GO:0003712	transcription coregulator activity
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity
molecular_function	GO:0003713	transcription coactivator activity