Cla97C06G113960.1 (mRNA) Watermelon (97103) v2.5

Overview


Name	Cla97C06G113960.1
Type	mRNA
Organism	Citrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
Description	Ubiquitin-conjugating enzyme family protein
Location	Cla97Chr06: 4916144 .. 4917070 (-)
Sequence length	927
RNA-Seq Expression	Cla97C06G113960.1
Synteny	Cla97C06G113960.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCTCCTCCTCTCTGCTATTCACACGACGGCAAAGACAAACACCCGGCCACCGTCGACGGCGTTGACGAACCTGACAGATCGCCTTCTCCGCCGCCGCCATCGTTCTCCTTCAAACGGTTCGATATCGTCACCGACTACTCCGATCACCATTACGCCGATTCAAACTTGTTCGCGAACTGCATTGGATCAGATGTCTCAAAGAAGATCATGCGAGAATGGAAGATTCTAGAGAGAAATCTTCCGGAATCGATTTTCGTTCGTGCTTACGAAGAGAGAATCGATCTTCTACGAGCAGTGATCGTAGGTCCAGCCGGTACTCCGTATCACGACGGATTGTTCTTCTTCGATCTAGCTTTCACTTCCGATTATCCGATTAGTCCTCCGTTGGTGTATTACCACTCATACGGACTTAGATTAAACCCTAACCTGTACGAAGACGGCTATGTCTGTTTGAGTTTGATAAATACGTGGGATGGAAAAGAAGAAGAGAGATGGAATCCAGCGATTTCGACAGTTTTACAGATTTTAATATCGATTCAAGCGCTGGTGTTGAACGAGAAGCCGTATTTCAACGAACCGGAACTAGAAGAAGAAGCGGAGGCAGAGATTTACTCAGATGCTTACAATAAGGAAGCGTACTTGTTCTGTTGCAAGATGATGATGAGATTGATGAAGAATCCGCCGAAGAATTTCAAGGAATTTGTGAGGAATTACTTTGGGGAGAAAGGGAATAAGATACTGGCGGCGTGCAAGGCTTATTCGGACGGCCGGATTAGGGTTGGGCGGTTCAATGAAAAGGAAAATAGTGAAAGTGAAAGTGATTATTGTAACAAAGCGGACGTTTCTGCCATTTTTAAGGCGTTAATGGAGAAGATGCATCGGGATCTGCACGCAGCTTTCCTCCTCATCTGCACCTCCTAG

mRNA sequence

ATGGCTTCTCCTCCTCTCTGCTATTCACACGACGGCAAAGACAAACACCCGGCCACCGTCGACGGCGTTGACGAACCTGACAGATCGCCTTCTCCGCCGCCGCCATCGTTCTCCTTCAAACGGTTCGATATCGTCACCGACTACTCCGATCACCATTACGCCGATTCAAACTTGTTCGCGAACTGCATTGGATCAGATGTCTCAAAGAAGATCATGCGAGAATGGAAGATTCTAGAGAGAAATCTTCCGGAATCGATTTTCGTTCGTGCTTACGAAGAGAGAATCGATCTTCTACGAGCAGTGATCGTAGGTCCAGCCGGTACTCCGTATCACGACGGATTGTTCTTCTTCGATCTAGCTTTCACTTCCGATTATCCGATTAGTCCTCCGTTGGTGTATTACCACTCATACGGACTTAGATTAAACCCTAACCTGTACGAAGACGGCTATGTCTGTTTGAGTTTGATAAATACGTGGGATGGAAAAGAAGAAGAGAGATGGAATCCAGCGATTTCGACAGTTTTACAGATTTTAATATCGATTCAAGCGCTGGTGTTGAACGAGAAGCCGTATTTCAACGAACCGGAACTAGAAGAAGAAGCGGAGGCAGAGATTTACTCAGATGCTTACAATAAGGAAGCGTACTTGTTCTGTTGCAAGATGATGATGAGATTGATGAAGAATCCGCCGAAGAATTTCAAGGAATTTGTGAGGAATTACTTTGGGGAGAAAGGGAATAAGATACTGGCGGCGTGCAAGGCTTATTCGGACGGCCGGATTAGGGTTGGGCGGTTCAATGAAAAGGAAAATAGTGAAAGTGAAAGTGATTATTGTAACAAAGCGGACGTTTCTGCCATTTTTAAGGCGTTAATGGAGAAGATGCATCGGGATCTGCACGCAGCTTTCCTCCTCATCTGCACCTCCTAG

Coding sequence (CDS)

ATGGCTTCTCCTCCTCTCTGCTATTCACACGACGGCAAAGACAAACACCCGGCCACCGTCGACGGCGTTGACGAACCTGACAGATCGCCTTCTCCGCCGCCGCCATCGTTCTCCTTCAAACGGTTCGATATCGTCACCGACTACTCCGATCACCATTACGCCGATTCAAACTTGTTCGCGAACTGCATTGGATCAGATGTCTCAAAGAAGATCATGCGAGAATGGAAGATTCTAGAGAGAAATCTTCCGGAATCGATTTTCGTTCGTGCTTACGAAGAGAGAATCGATCTTCTACGAGCAGTGATCGTAGGTCCAGCCGGTACTCCGTATCACGACGGATTGTTCTTCTTCGATCTAGCTTTCACTTCCGATTATCCGATTAGTCCTCCGTTGGTGTATTACCACTCATACGGACTTAGATTAAACCCTAACCTGTACGAAGACGGCTATGTCTGTTTGAGTTTGATAAATACGTGGGATGGAAAAGAAGAAGAGAGATGGAATCCAGCGATTTCGACAGTTTTACAGATTTTAATATCGATTCAAGCGCTGGTGTTGAACGAGAAGCCGTATTTCAACGAACCGGAACTAGAAGAAGAAGCGGAGGCAGAGATTTACTCAGATGCTTACAATAAGGAAGCGTACTTGTTCTGTTGCAAGATGATGATGAGATTGATGAAGAATCCGCCGAAGAATTTCAAGGAATTTGTGAGGAATTACTTTGGGGAGAAAGGGAATAAGATACTGGCGGCGTGCAAGGCTTATTCGGACGGCCGGATTAGGGTTGGGCGGTTCAATGAAAAGGAAAATAGTGAAAGTGAAAGTGATTATTGTAACAAAGCGGACGTTTCTGCCATTTTTAAGGCGTTAATGGAGAAGATGCATCGGGATCTGCACGCAGCTTTCCTCCTCATCTGCACCTCCTAG

Protein sequence

MASPPLCYSHDGKDKHPATVDGVDEPDRSPSPPPPSFSFKRFDIVTDYSDHHYADSNLFANCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFTSDYPISPPLVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILISIQALVLNEKPYFNEPELEEEAEAEIYSDAYNKEAYLFCCKMMMRLMKNPPKNFKEFVRNYFGEKGNKILAACKAYSDGRIRVGRFNEKENSESESDYCNKADVSAIFKALMEKMHRDLHAAFLLICTS

Homology

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BLAST of Cla97C06G113960.1 vs. NCBI nr
Match: XP_038907021.1 (probable ubiquitin-conjugating enzyme E2 25 [Benincasa hispida])

HSP 1 Score: 582.0 bits (1499), Expect = 3.0e-162
Identity = 280/306 (91.50%), Postives = 292/306 (95.42%), Query Frame = 0


Query: 2   ASPPLCYSHDGKDKHPATVDGVDEPDRSPSPPPPSFSFKRFDIVTDYSDHHYADSNLFAN 61
           +S PLCYSHDG+DKHPAT DG DEP  + SPPP  FSFK+FDIV+DYSDHHYADSNLFAN
Sbjct: 5   SSSPLCYSHDGEDKHPATEDGGDEP--NGSPPPTPFSFKQFDIVSDYSDHHYADSNLFAN 64

Query: 62  CIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAF 121
           CIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAF
Sbjct: 65  CIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAF 124

Query: 122 TSDYPISPPLVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILISI 181
           TSDYP SPP+VYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILISI
Sbjct: 125 TSDYPNSPPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILISI 184

Query: 182 QALVLNEKPYFNEPELEEEAEAEIYSDAYNKEAYLFCCKMMMRLMKNPPKNFKEFVRNYF 241
           QALVLNEKPYFNEPELEEE EAEIYSDAYNKEAYLFCCKMMMRLMKNPPKN++EFV+NYF
Sbjct: 185 QALVLNEKPYFNEPELEEEVEAEIYSDAYNKEAYLFCCKMMMRLMKNPPKNYEEFVKNYF 244

Query: 242 GEKGNKILAACKAYSDGRIRVGRFNEKENSESESDYCNKADVSAIFKALMEKMHRDLHAA 301
           GEKG  ILAACKAYSDGR+RVGRFNEKENSESESD CN+A+VSAIFKALMEKMHRDLHAA
Sbjct: 245 GEKGKGILAACKAYSDGRVRVGRFNEKENSESESDDCNRAEVSAIFKALMEKMHRDLHAA 304

Query: 302 FLLICT 308
           FLL CT
Sbjct: 305 FLLFCT 308

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BLAST of Cla97C06G113960.1 vs. NCBI nr
Match: XP_008458344.1 (PREDICTED: putative ubiquitin-conjugating enzyme E2 39 [Cucumis melo] >TYK02958.1 putative ubiquitin-conjugating enzyme E2 39 [Cucumis melo var. makuwa])

HSP 1 Score: 531.2 bits (1367), Expect = 6.0e-147
Identity = 262/320 (81.88%), Postives = 281/320 (87.81%), Query Frame = 0


Query: 3   SPPLCYSHDGKDKHPATVDGVDEPDR---SPSPPPPSFSFKRFDIVTDYSDHHYADSNLF 62
           SPPL    DG+DKHP TVDG DEP+R   SPSP PPSFSFKRFDIV+DYSDHHYADSNLF
Sbjct: 9   SPPL----DGEDKHPTTVDGGDEPNRSLSSPSPSPPSFSFKRFDIVSDYSDHHYADSNLF 68

Query: 63  ANCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDL 122
            NCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGP+GTPYHDGLFFFDL
Sbjct: 69  PNCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPSGTPYHDGLFFFDL 128

Query: 123 AFTSDYPISPPLVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILI 182
           AFTSDYPI+PP+VY HSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNP+ISTVLQILI
Sbjct: 129 AFTSDYPITPPMVYCHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPSISTVLQILI 188

Query: 183 SIQALVLNEKPYFNEPELEEEA---EAEIYSDAYNKEAYLFCCKMMMRLMKNPPKNFKEF 242
           SIQALVLNEKPYFNEPELEEE    + EIYSDAYNKE YL CC+MMMRLMKNP KN++EF
Sbjct: 189 SIQALVLNEKPYFNEPELEEEEADDQGEIYSDAYNKEVYLLCCEMMMRLMKNPVKNYEEF 248

Query: 243 VRNYFGEKGNKILAACKAYSDGRIRVGRFNEKE--------NSESESDYCNKADVSAIFK 302
           VRNYFGEKG +ILAAC+AYSDGR+RVGR+NE E        N+ +  + C + DVSAIFK
Sbjct: 249 VRNYFGEKGERILAACEAYSDGRVRVGRYNENEKENNNINNNNNNNGETCKRGDVSAIFK 308

Query: 303 ALMEKMHRDLHAAFLLICTS 309
           ALMEK HRDLHAAFL  CTS
Sbjct: 309 ALMEKTHRDLHAAFLHFCTS 324

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BLAST of Cla97C06G113960.1 vs. NCBI nr
Match: XP_004148800.1 (probable ubiquitin-conjugating enzyme E2 25 [Cucumis sativus] >KGN47459.1 hypothetical protein Csa_021762 [Cucumis sativus])

HSP 1 Score: 529.3 bits (1362), Expect = 2.3e-146
Identity = 261/317 (82.33%), Postives = 281/317 (88.64%), Query Frame = 0


Query: 1   MASPPLCYSH---DGKDKHPATVDGVDEPDRS-PSPPPPSFSFKRFDIVTDYSDHHYADS 60
           MASP L  S    DG+DKHP TVDG DEP+RS  SP PPSFSFKRFDIVTDYSDHHYADS
Sbjct: 1   MASPSLPPSPPPLDGEDKHPTTVDGGDEPNRSLSSPSPPSFSFKRFDIVTDYSDHHYADS 60

Query: 61  NLFANCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFF 120
           NLF NCIGSDVSKKIMREWK+LERNLPESIFVRAYEERIDLLRAVIVGP+GTPYHDGLFF
Sbjct: 61  NLFPNCIGSDVSKKIMREWKVLERNLPESIFVRAYEERIDLLRAVIVGPSGTPYHDGLFF 120

Query: 121 FDLAFTSDYPISPPLVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQ 180
           FDLAFTSDYP +PP+VYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNP+ISTVLQ
Sbjct: 121 FDLAFTSDYPNTPPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPSISTVLQ 180

Query: 181 ILISIQALVLNEKPYFNEPELE--EEAEAEIYSDAYNKEAYLFCCKMMMRLMKNPPKNFK 240
           ILISIQ LVLNEKPYFNEPELE  E  + EIYSDAYNKE YL CC+MMMRLMKNP KN++
Sbjct: 181 ILISIQGLVLNEKPYFNEPELEKDEADQGEIYSDAYNKEVYLLCCEMMMRLMKNPVKNYE 240

Query: 241 EFVRNYFGEKGNKILAACKAYSDGRIRVGRFNEKENSESES---DYCNKADVSAIFKALM 300
           EFVRNYFG KG +ILAAC+AYSDGR+RVGR+NEKEN+ + +   + C + DVSAIFK LM
Sbjct: 241 EFVRNYFGGKGERILAACEAYSDGRVRVGRYNEKENNNNNNNNGENCKRGDVSAIFKTLM 300

Query: 301 EKMHRDLHAAFLLICTS 309
           EKMHRDLHAAFL  CTS
Sbjct: 301 EKMHRDLHAAFLHFCTS 317

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BLAST of Cla97C06G113960.1 vs. NCBI nr
Match: XP_022930350.1 (probable ubiquitin-conjugating enzyme E2 25 [Cucurbita moschata])

HSP 1 Score: 489.2 bits (1258), Expect = 2.6e-134
Identity = 245/302 (81.13%), Postives = 263/302 (87.09%), Query Frame = 0


Query: 6   LCYSHDGKDKHPATVDGVDEPDRSPSPPPPSFSFKRFDIVTDYSDHHYADSNLFANCIGS 65
           LCYSHD +DK P T     EP+RSP        FKRFDIVTD+SDHHYA SNLF NCIGS
Sbjct: 5   LCYSHDAEDKQPPT---ALEPERSP-------PFKRFDIVTDFSDHHYAHSNLFPNCIGS 64

Query: 66  DVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFTSDY 125
           DVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAF+SDY
Sbjct: 65  DVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSDY 124

Query: 126 PISPPLVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILISIQALV 185
           PI PP+VYYHSYGLRLNPNLYEDGYVCLSLINTWDGK+EERWNPAISTVLQILISIQALV
Sbjct: 125 PICPPIVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQALV 184

Query: 186 LNEKPYFNEPELEEEAEAEIYSDAYNKEAYLFCCKMMMRLMKNPPKNFKEFVRNYFGEKG 245
           LNE+PYFNEPEL+E AEAEIYSDAYNKEAYLFCCKM MRL++NPPKNF+EFVR+YF ++G
Sbjct: 185 LNERPYFNEPELDEAAEAEIYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQRG 244

Query: 246 NKILAACKAYSDGRIRVGRF--NEKEN---SESESDYCNKADVSAIFKALMEKMHRDLHA 303
             ILAACKAYS+GR+RVGRF  +E EN   S S S+  N   VSAIFKALMEK HRDL A
Sbjct: 245 KGILAACKAYSEGRVRVGRFSGSETENCSWSSSSSNGGNGTHVSAIFKALMEKTHRDLQA 296

[Close]

BLAST of Cla97C06G113960.1 vs. NCBI nr
Match: XP_022999956.1 (probable ubiquitin-conjugating enzyme E2 25 [Cucurbita maxima])

HSP 1 Score: 488.8 bits (1257), Expect = 3.4e-134
Identity = 245/302 (81.13%), Postives = 263/302 (87.09%), Query Frame = 0


Query: 6   LCYSHDGKDKHPATVDGVDEPDRSPSPPPPSFSFKRFDIVTDYSDHHYADSNLFANCIGS 65
           LCYSH  +DK P T     EP+RSP        FKRFDIVTD+SDHHYA SNLF NCIGS
Sbjct: 5   LCYSHHAEDKQPPT---PHEPERSP-------PFKRFDIVTDFSDHHYAHSNLFPNCIGS 64

Query: 66  DVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFTSDY 125
           DVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAF+SDY
Sbjct: 65  DVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSDY 124

Query: 126 PISPPLVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILISIQALV 185
           PISPP+VYYHSYGLRLNPNLYEDGYVCLSLINTWDGK+EERWNPAISTVLQILISIQALV
Sbjct: 125 PISPPIVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQALV 184

Query: 186 LNEKPYFNEPELEEEAEAEIYSDAYNKEAYLFCCKMMMRLMKNPPKNFKEFVRNYFGEKG 245
           LNE+PYFNEPEL+E AEAEIYSDAYNKEAYLFCCKM MRL++NPPKNF+EFVR+YF ++G
Sbjct: 185 LNERPYFNEPELDEAAEAEIYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQRG 244

Query: 246 NKILAACKAYSDGRIRVGRF--NEKEN---SESESDYCNKADVSAIFKALMEKMHRDLHA 303
             ILAACKAYS+GR+RVGRF  +E EN   S S S+  N   VSAIFKALMEK HRDL A
Sbjct: 245 KGILAACKAYSEGRVRVGRFSGSETENCSWSSSSSNGGNGTHVSAIFKALMEKTHRDLQA 296

[Close]

BLAST of Cla97C06G113960.1 vs. ExPASy Swiss-Prot
Match: Q9LUQ5 (Probable ubiquitin-conjugating enzyme E2 25 OS=Arabidopsis thaliana OX=3702 GN=UBC25 PE=2 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 5.2e-69
Identity = 129/262 (49.24%), Postives = 171/262 (65.27%), Query Frame = 0


Query: 39  FKRFDIVTDYSDHHYADSNLFANCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLL 98
           FKRFDIV D+SDHHYA     +     D +K+I  EW+ILE++LPE IFVRAYE R+DLL
Sbjct: 305 FKRFDIVEDFSDHHYASKGTTSKQHSKDWAKRIQDEWRILEKDLPEMIFVRAYESRMDLL 364

Query: 99  RAVIVGPAGTPYHDGLFFFDLAFTSDYPISPPLVYYHSYGLRLNPNLYEDGYVCLSLINT 158
           RAVI+G  GTPYHDGLFFFD+ F   YP +PP+V+YHS GLR+NPNLY  G VCLSL+ T
Sbjct: 365 RAVIIGAQGTPYHDGLFFFDIFFPDTYPSTPPIVHYHSGGLRINPNLYNCGKVCLSLLGT 424

Query: 159 WDGKEEERWNPAISTVLQILISIQALVLNEKPYFNEPELEE---EAEAEIYSDAYNKEAY 218
           W G + E+W P  ST+LQ+L+SIQ L+LN+KPYFNEP  E     A  E  S AY++  +
Sbjct: 425 WSGNQREKWIPNTSTMLQVLVSIQGLILNQKPYFNEPGYERSAGSAHGESTSKAYSENTF 484

Query: 219 LFCCKMMMRLMKNPPKNFKEFVRNYFGEKGNKILAACKAYSDGR----IRVGRFNEKENS 278
           +   K M+  M+ PPK F++F   +F    + +L AC AY +G     +  G  + +ENS
Sbjct: 485 ILSLKTMVYTMRRPPKYFEDFAYGHFFSCAHDVLKACNAYRNGATPGYLVKGAPDVEENS 544

Query: 279 ESESDYCNKADVSAIFKALMEK 294
              S    + DV+   + ++ K
Sbjct: 545 AGMSSLKFRTDVATFVETVLLK 566

[Close]

BLAST of Cla97C06G113960.1 vs. ExPASy Swiss-Prot
Match: Q8VY10 (Probable ubiquitin-conjugating enzyme E2 24 OS=Arabidopsis thaliana OX=3702 GN=UBC24 PE=1 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 8.4e-67
Identity = 129/269 (47.96%), Postives = 175/269 (65.06%), Query Frame = 0


Query: 39  FKRFDIVTDYSDHHY--ADSNLFANCIGSDVSKKIMREWKILERNLPESIFVRAYEERID 98
           F++FD+V D SDHH+  +D  L  + +     KK+ +EW  LE NLP +I+VR  EER+D
Sbjct: 633 FRQFDMVNDCSDHHFLSSDKGLAQSQVTKSWVKKVQQEWSNLEANLPNTIYVRVCEERMD 692

Query: 99  LLRAVIVGPAGTPYHDGLFFFDLAFTSDYPISPPLVYYHSYGLRLNPNLYEDGYVCLSLI 158
           LLRA +VG  GTPYHDGLFFFD+     YP  PP+V+YHS G+RLNPNLYE G VCLSL+
Sbjct: 693 LLRAALVGAPGTPYHDGLFFFDIMLPPQYPHEPPMVHYHSGGMRLNPNLYESGRVCLSLL 752

Query: 159 NTWDGKEEERWNPAISTVLQILISIQALVLNEKPYFNEPELEEE---AEAEIYSDAYNKE 218
           NTW G   E WN   S++LQ+L+S QALVLNEKPYFNE   +++   AE E  S +YN+ 
Sbjct: 753 NTWSGSGTEVWNAGSSSILQLLLSFQALVLNEKPYFNEAGYDKQLGRAEGEKNSVSYNEN 812

Query: 219 AYLFCCKMMMRLMKNPPKNFKEFVRNYFGEKGNKILAACKAYSDGRIRVGRFNEKENSES 278
           A+L  CK M+ +++ PPK+F+  V+++F  +   +LAACKAY +G + VG         S
Sbjct: 813 AFLITCKSMISMLRKPPKHFEMLVKDHFTHRAQHVLAACKAYMEG-VPVG--------SS 872

Query: 279 ESDYCNKADVSAIFKALMEKMHRDLHAAF 303
            +   N    S  FK ++ K++  L  AF
Sbjct: 873 ANLQGNSTTNSTGFKIMLSKLYPKLLEAF 892

[Close]

BLAST of Cla97C06G113960.1 vs. ExPASy Swiss-Prot
Match: Q9ZVX1 (Probable ubiquitin-conjugating enzyme E2 23 OS=Arabidopsis thaliana OX=3702 GN=UBC23 PE=1 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 5.4e-66
Identity = 135/287 (47.04%), Postives = 178/287 (62.02%), Query Frame = 0


Query: 23   VDEPDRSPSPPPPSFSFKRFDIVTDYSDHHYADSNLFANCIGSDVS-----KKIMREWKI 82
            +++ D+       S SF+RFDI  D  DHH      F    G         KK+ ++WKI
Sbjct: 808  LEDEDKPVPSEGDSCSFRRFDISQDPLDHH------FLGVDGQKTKERQWFKKVDQDWKI 867

Query: 83   LERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFTSDYPISPPLVYYHSY 142
            L+ NLP+ IFVRAYE+R+DLLRAVIVG  GTPY DGLFFFD    SDYP  PP  YYHS 
Sbjct: 868  LQNNLPDGIFVRAYEDRMDLLRAVIVGAFGTPYQDGLFFFDFHLPSDYPSVPPSAYYHSG 927

Query: 143  GLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILISIQALVLNEKPYFNEPEL 202
            G RLNPNLYE+G VCLSL+NTW G+  E W+P  S++LQ+L+S+Q LVLN KPYFNE   
Sbjct: 928  GWRLNPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGY 987

Query: 203  EEE---AEAEIYSDAYNKEAYLFCCKMMMRLMKNPPKNFKEFVRNYFGEKGNKILAACKA 262
            +++   AE E  S  YN+  +L  CK MM LM+ PPK+F+E ++++F ++G  IL AC A
Sbjct: 988  DKQVGTAEGEKNSLGYNENTFLLNCKTMMYLMRKPPKDFEELIKDHFRKRGYYILKACDA 1047

Query: 263  YSDGRIRVGRFNEKENSESESDYCNKADVSAIFKALMEKMHRDLHAA 302
            Y  G + +G   +  +   E    N   V   FK ++ K+   L +A
Sbjct: 1048 YMKGYL-IGSLTKDASVIDERSSANSTSVG--FKLMLAKIAPKLFSA 1085

[Close]

BLAST of Cla97C06G113960.1 vs. ExPASy Swiss-Prot
Match: Q9C918 (Putative ubiquitin-conjugating enzyme E2 38 OS=Arabidopsis thaliana OX=3702 GN=UBC38 PE=3 SV=2)

HSP 1 Score: 245.7 bits (626), Expect = 6.6e-64
Identity = 123/263 (46.77%), Postives = 167/263 (63.50%), Query Frame = 0


Query: 38  SFKRFDIVTDYSDHHYADSNLFANCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDL 97
           +FKRFD V D+SDHHY      +     +  KK+  EWKIL +NLPE+IFVRA E R+DL
Sbjct: 26  NFKRFDSVEDFSDHHYVSKGKASKQHSKNWVKKVQDEWKILNQNLPETIFVRACESRMDL 85

Query: 98  LRAVIVGPAGTPYHDGLFFFDLAFTSDYPISPPLVYYHSYGLRLNPNLYEDGYVCLSLIN 157
           LRAVI+G  GTPYHDGLFFFD+ F   YP  PP VYYHS GLR+NPNLY  G VCLSL+ 
Sbjct: 86  LRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPNVYYHSGGLRINPNLYNCGKVCLSLLG 145

Query: 158 TWDGKEEERWNPAISTVLQILISIQALVLNEKPYFNEPE---LEEEAEAEIYSDAYNKEA 217
           TW G   + W P  ST+LQ+L+SIQALVLNE+PYFNEP    ++     E  S  Y++  
Sbjct: 146 TWHGNARQSWLPKESTMLQVLVSIQALVLNEQPYFNEPGYGLIKGTWLGESKSKVYSENV 205

Query: 218 YLFCCKMMMRLMKNPPKNFKEFVRNYFGEKGNKILAACKAYSD----GRIRVGRFNEKEN 277
           +L   K M+  M+ PP++F+E+V+N++  + + I+ AC AY      G +  G   + E 
Sbjct: 206 FLLSLKTMVYSMRKPPQHFEEYVQNHYFVRSHDIVKACNAYKAGAPLGSMVKGGVQDLEQ 265

Query: 278 SESESDYCNKADVSAIFKALMEK 294
           +        K DV++  + ++++
Sbjct: 266 ARQSGSKKFKTDVASFMQTVVDE 288

[Close]

BLAST of Cla97C06G113960.1 vs. ExPASy Swiss-Prot
Match: Q8GY87 (Probable ubiquitin-conjugating enzyme E2 26 OS=Arabidopsis thaliana OX=3702 GN=UBC26 PE=2 SV=2)

HSP 1 Score: 244.2 bits (622), Expect = 1.9e-63
Identity = 127/300 (42.33%), Postives = 175/300 (58.33%), Query Frame = 0


Query: 5   PLCYSHDGKDKHPATVDGVDEPDRSPSPPPPS----FSFKRFDIVTDYSDHHYADSNLFA 64
           P  Y       HP   + +     S +           FKRFD V D+SDHHYA     +
Sbjct: 206 PFSYDASASSSHPIAAEPISSVQDSSNARKLKEEFLRDFKRFDTVEDFSDHHYASKGKSS 265

Query: 65  NCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLA 124
                +  KK+  +WKILE +LPE+I VRA E R+DLLRAVI+G  GTPYHDGLFFFD+ 
Sbjct: 266 KQHSKNWVKKVQADWKILENDLPEAISVRACESRMDLLRAVIIGAEGTPYHDGLFFFDIQ 325

Query: 125 FTSDYPISPPLVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILIS 184
           F   YP  PP V+YHS GLR+NPNLY  G VCLSL+ TW G   E+W P  ST+LQ+L+S
Sbjct: 326 FPDTYPSVPPNVHYHSGGLRINPNLYNCGKVCLSLLGTWAGSAREKWLPNESTMLQLLVS 385

Query: 185 IQALVLNEKPYFNEPELEEE---AEAEIYSDAYNKEAYLFCCKMMMRLMKNPPKNFKEFV 244
           IQAL+LNEKPYFNEP   +    A  E  S  Y++  +L   K M+  ++ PP++F+E+V
Sbjct: 386 IQALILNEKPYFNEPGYVQSAGTASGESKSKVYSENVFLLSLKTMVYSIRRPPQHFEEYV 445

Query: 245 RNYFGEKGNKILAACKAYSD----GRIRVGRFNEKENSESESDYCNKADVSAIFKALMEK 294
           +N++  + + I+ AC AY      G +  G   + E +        K DV++  + ++++
Sbjct: 446 QNHYFVRSHDIVKACNAYKAGAPLGSMVKGGVQDLEEARQSGSKKFKTDVASFMQTVVDE 505

[Close]

BLAST of Cla97C06G113960.1 vs. ExPASy TrEMBL
Match: A0A5D3BVE0 (Putative ubiquitin-conjugating enzyme E2 39 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G00410 PE=4 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 2.9e-147
Identity = 262/320 (81.88%), Postives = 281/320 (87.81%), Query Frame = 0


Query: 3   SPPLCYSHDGKDKHPATVDGVDEPDR---SPSPPPPSFSFKRFDIVTDYSDHHYADSNLF 62
           SPPL    DG+DKHP TVDG DEP+R   SPSP PPSFSFKRFDIV+DYSDHHYADSNLF
Sbjct: 9   SPPL----DGEDKHPTTVDGGDEPNRSLSSPSPSPPSFSFKRFDIVSDYSDHHYADSNLF 68

Query: 63  ANCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDL 122
            NCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGP+GTPYHDGLFFFDL
Sbjct: 69  PNCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPSGTPYHDGLFFFDL 128

Query: 123 AFTSDYPISPPLVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILI 182
           AFTSDYPI+PP+VY HSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNP+ISTVLQILI
Sbjct: 129 AFTSDYPITPPMVYCHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPSISTVLQILI 188

Query: 183 SIQALVLNEKPYFNEPELEEEA---EAEIYSDAYNKEAYLFCCKMMMRLMKNPPKNFKEF 242
           SIQALVLNEKPYFNEPELEEE    + EIYSDAYNKE YL CC+MMMRLMKNP KN++EF
Sbjct: 189 SIQALVLNEKPYFNEPELEEEEADDQGEIYSDAYNKEVYLLCCEMMMRLMKNPVKNYEEF 248

Query: 243 VRNYFGEKGNKILAACKAYSDGRIRVGRFNEKE--------NSESESDYCNKADVSAIFK 302
           VRNYFGEKG +ILAAC+AYSDGR+RVGR+NE E        N+ +  + C + DVSAIFK
Sbjct: 249 VRNYFGEKGERILAACEAYSDGRVRVGRYNENEKENNNINNNNNNNGETCKRGDVSAIFK 308

Query: 303 ALMEKMHRDLHAAFLLICTS 309
           ALMEK HRDLHAAFL  CTS
Sbjct: 309 ALMEKTHRDLHAAFLHFCTS 324

[Close]

BLAST of Cla97C06G113960.1 vs. ExPASy TrEMBL
Match: A0A1S3C8W9 (putative ubiquitin-conjugating enzyme E2 39 OS=Cucumis melo OX=3656 GN=LOC103497784 PE=4 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 2.9e-147
Identity = 262/320 (81.88%), Postives = 281/320 (87.81%), Query Frame = 0


Query: 3   SPPLCYSHDGKDKHPATVDGVDEPDR---SPSPPPPSFSFKRFDIVTDYSDHHYADSNLF 62
           SPPL    DG+DKHP TVDG DEP+R   SPSP PPSFSFKRFDIV+DYSDHHYADSNLF
Sbjct: 9   SPPL----DGEDKHPTTVDGGDEPNRSLSSPSPSPPSFSFKRFDIVSDYSDHHYADSNLF 68

Query: 63  ANCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDL 122
            NCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGP+GTPYHDGLFFFDL
Sbjct: 69  PNCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPSGTPYHDGLFFFDL 128

Query: 123 AFTSDYPISPPLVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILI 182
           AFTSDYPI+PP+VY HSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNP+ISTVLQILI
Sbjct: 129 AFTSDYPITPPMVYCHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPSISTVLQILI 188

Query: 183 SIQALVLNEKPYFNEPELEEEA---EAEIYSDAYNKEAYLFCCKMMMRLMKNPPKNFKEF 242
           SIQALVLNEKPYFNEPELEEE    + EIYSDAYNKE YL CC+MMMRLMKNP KN++EF
Sbjct: 189 SIQALVLNEKPYFNEPELEEEEADDQGEIYSDAYNKEVYLLCCEMMMRLMKNPVKNYEEF 248

Query: 243 VRNYFGEKGNKILAACKAYSDGRIRVGRFNEKE--------NSESESDYCNKADVSAIFK 302
           VRNYFGEKG +ILAAC+AYSDGR+RVGR+NE E        N+ +  + C + DVSAIFK
Sbjct: 249 VRNYFGEKGERILAACEAYSDGRVRVGRYNENEKENNNINNNNNNNGETCKRGDVSAIFK 308

Query: 303 ALMEKMHRDLHAAFLLICTS 309
           ALMEK HRDLHAAFL  CTS
Sbjct: 309 ALMEKTHRDLHAAFLHFCTS 324

[Close]

BLAST of Cla97C06G113960.1 vs. ExPASy TrEMBL
Match: A0A0A0KF71 (UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G330470 PE=4 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 1.1e-146
Identity = 261/317 (82.33%), Postives = 281/317 (88.64%), Query Frame = 0


Query: 1   MASPPLCYSH---DGKDKHPATVDGVDEPDRS-PSPPPPSFSFKRFDIVTDYSDHHYADS 60
           MASP L  S    DG+DKHP TVDG DEP+RS  SP PPSFSFKRFDIVTDYSDHHYADS
Sbjct: 1   MASPSLPPSPPPLDGEDKHPTTVDGGDEPNRSLSSPSPPSFSFKRFDIVTDYSDHHYADS 60

Query: 61  NLFANCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFF 120
           NLF NCIGSDVSKKIMREWK+LERNLPESIFVRAYEERIDLLRAVIVGP+GTPYHDGLFF
Sbjct: 61  NLFPNCIGSDVSKKIMREWKVLERNLPESIFVRAYEERIDLLRAVIVGPSGTPYHDGLFF 120

Query: 121 FDLAFTSDYPISPPLVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQ 180
           FDLAFTSDYP +PP+VYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNP+ISTVLQ
Sbjct: 121 FDLAFTSDYPNTPPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPSISTVLQ 180

Query: 181 ILISIQALVLNEKPYFNEPELE--EEAEAEIYSDAYNKEAYLFCCKMMMRLMKNPPKNFK 240
           ILISIQ LVLNEKPYFNEPELE  E  + EIYSDAYNKE YL CC+MMMRLMKNP KN++
Sbjct: 181 ILISIQGLVLNEKPYFNEPELEKDEADQGEIYSDAYNKEVYLLCCEMMMRLMKNPVKNYE 240

Query: 241 EFVRNYFGEKGNKILAACKAYSDGRIRVGRFNEKENSESES---DYCNKADVSAIFKALM 300
           EFVRNYFG KG +ILAAC+AYSDGR+RVGR+NEKEN+ + +   + C + DVSAIFK LM
Sbjct: 241 EFVRNYFGGKGERILAACEAYSDGRVRVGRYNEKENNNNNNNNGENCKRGDVSAIFKTLM 300

Query: 301 EKMHRDLHAAFLLICTS 309
           EKMHRDLHAAFL  CTS
Sbjct: 301 EKMHRDLHAAFLHFCTS 317

[Close]

BLAST of Cla97C06G113960.1 vs. ExPASy TrEMBL
Match: A0A6J1EWP5 (probable ubiquitin-conjugating enzyme E2 25 OS=Cucurbita moschata OX=3662 GN=LOC111436820 PE=4 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 1.3e-134
Identity = 245/302 (81.13%), Postives = 263/302 (87.09%), Query Frame = 0


Query: 6   LCYSHDGKDKHPATVDGVDEPDRSPSPPPPSFSFKRFDIVTDYSDHHYADSNLFANCIGS 65
           LCYSHD +DK P T     EP+RSP        FKRFDIVTD+SDHHYA SNLF NCIGS
Sbjct: 5   LCYSHDAEDKQPPT---ALEPERSP-------PFKRFDIVTDFSDHHYAHSNLFPNCIGS 64

Query: 66  DVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFTSDY 125
           DVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAF+SDY
Sbjct: 65  DVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSDY 124

Query: 126 PISPPLVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILISIQALV 185
           PI PP+VYYHSYGLRLNPNLYEDGYVCLSLINTWDGK+EERWNPAISTVLQILISIQALV
Sbjct: 125 PICPPIVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQALV 184

Query: 186 LNEKPYFNEPELEEEAEAEIYSDAYNKEAYLFCCKMMMRLMKNPPKNFKEFVRNYFGEKG 245
           LNE+PYFNEPEL+E AEAEIYSDAYNKEAYLFCCKM MRL++NPPKNF+EFVR+YF ++G
Sbjct: 185 LNERPYFNEPELDEAAEAEIYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQRG 244

Query: 246 NKILAACKAYSDGRIRVGRF--NEKEN---SESESDYCNKADVSAIFKALMEKMHRDLHA 303
             ILAACKAYS+GR+RVGRF  +E EN   S S S+  N   VSAIFKALMEK HRDL A
Sbjct: 245 KGILAACKAYSEGRVRVGRFSGSETENCSWSSSSSNGGNGTHVSAIFKALMEKTHRDLQA 296

[Close]

BLAST of Cla97C06G113960.1 vs. ExPASy TrEMBL
Match: A0A6J1KEJ5 (probable ubiquitin-conjugating enzyme E2 25 OS=Cucurbita maxima OX=3661 GN=LOC111494283 PE=4 SV=1)

HSP 1 Score: 488.8 bits (1257), Expect = 1.7e-134
Identity = 245/302 (81.13%), Postives = 263/302 (87.09%), Query Frame = 0


Query: 6   LCYSHDGKDKHPATVDGVDEPDRSPSPPPPSFSFKRFDIVTDYSDHHYADSNLFANCIGS 65
           LCYSH  +DK P T     EP+RSP        FKRFDIVTD+SDHHYA SNLF NCIGS
Sbjct: 5   LCYSHHAEDKQPPT---PHEPERSP-------PFKRFDIVTDFSDHHYAHSNLFPNCIGS 64

Query: 66  DVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFTSDY 125
           DVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAF+SDY
Sbjct: 65  DVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSDY 124

Query: 126 PISPPLVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILISIQALV 185
           PISPP+VYYHSYGLRLNPNLYEDGYVCLSLINTWDGK+EERWNPAISTVLQILISIQALV
Sbjct: 125 PISPPIVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQALV 184

Query: 186 LNEKPYFNEPELEEEAEAEIYSDAYNKEAYLFCCKMMMRLMKNPPKNFKEFVRNYFGEKG 245
           LNE+PYFNEPEL+E AEAEIYSDAYNKEAYLFCCKM MRL++NPPKNF+EFVR+YF ++G
Sbjct: 185 LNERPYFNEPELDEAAEAEIYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQRG 244

Query: 246 NKILAACKAYSDGRIRVGRF--NEKEN---SESESDYCNKADVSAIFKALMEKMHRDLHA 303
             ILAACKAYS+GR+RVGRF  +E EN   S S S+  N   VSAIFKALMEK HRDL A
Sbjct: 245 KGILAACKAYSEGRVRVGRFSGSETENCSWSSSSSNGGNGTHVSAIFKALMEKTHRDLQA 296

[Close]

BLAST of Cla97C06G113960.1 vs. TAIR 10
Match: AT3G15355.1 (ubiquitin-conjugating enzyme 25 )

HSP 1 Score: 262.7 bits (670), Expect = 3.7e-70
Identity = 129/262 (49.24%), Postives = 171/262 (65.27%), Query Frame = 0


Query: 39  FKRFDIVTDYSDHHYADSNLFANCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLL 98
           FKRFDIV D+SDHHYA     +     D +K+I  EW+ILE++LPE IFVRAYE R+DLL
Sbjct: 305 FKRFDIVEDFSDHHYASKGTTSKQHSKDWAKRIQDEWRILEKDLPEMIFVRAYESRMDLL 364

Query: 99  RAVIVGPAGTPYHDGLFFFDLAFTSDYPISPPLVYYHSYGLRLNPNLYEDGYVCLSLINT 158
           RAVI+G  GTPYHDGLFFFD+ F   YP +PP+V+YHS GLR+NPNLY  G VCLSL+ T
Sbjct: 365 RAVIIGAQGTPYHDGLFFFDIFFPDTYPSTPPIVHYHSGGLRINPNLYNCGKVCLSLLGT 424

Query: 159 WDGKEEERWNPAISTVLQILISIQALVLNEKPYFNEPELEE---EAEAEIYSDAYNKEAY 218
           W G + E+W P  ST+LQ+L+SIQ L+LN+KPYFNEP  E     A  E  S AY++  +
Sbjct: 425 WSGNQREKWIPNTSTMLQVLVSIQGLILNQKPYFNEPGYERSAGSAHGESTSKAYSENTF 484

Query: 219 LFCCKMMMRLMKNPPKNFKEFVRNYFGEKGNKILAACKAYSDGR----IRVGRFNEKENS 278
           +   K M+  M+ PPK F++F   +F    + +L AC AY +G     +  G  + +ENS
Sbjct: 485 ILSLKTMVYTMRRPPKYFEDFAYGHFFSCAHDVLKACNAYRNGATPGYLVKGAPDVEENS 544

Query: 279 ESESDYCNKADVSAIFKALMEK 294
              S    + DV+   + ++ K
Sbjct: 545 AGMSSLKFRTDVATFVETVLLK 566

[Close]

BLAST of Cla97C06G113960.1 vs. TAIR 10
Match: AT2G33770.1 (phosphate 2 )

HSP 1 Score: 255.4 bits (651), Expect = 5.9e-68
Identity = 129/269 (47.96%), Postives = 175/269 (65.06%), Query Frame = 0


Query: 39  FKRFDIVTDYSDHHY--ADSNLFANCIGSDVSKKIMREWKILERNLPESIFVRAYEERID 98
           F++FD+V D SDHH+  +D  L  + +     KK+ +EW  LE NLP +I+VR  EER+D
Sbjct: 633 FRQFDMVNDCSDHHFLSSDKGLAQSQVTKSWVKKVQQEWSNLEANLPNTIYVRVCEERMD 692

Query: 99  LLRAVIVGPAGTPYHDGLFFFDLAFTSDYPISPPLVYYHSYGLRLNPNLYEDGYVCLSLI 158
           LLRA +VG  GTPYHDGLFFFD+     YP  PP+V+YHS G+RLNPNLYE G VCLSL+
Sbjct: 693 LLRAALVGAPGTPYHDGLFFFDIMLPPQYPHEPPMVHYHSGGMRLNPNLYESGRVCLSLL 752

Query: 159 NTWDGKEEERWNPAISTVLQILISIQALVLNEKPYFNEPELEEE---AEAEIYSDAYNKE 218
           NTW G   E WN   S++LQ+L+S QALVLNEKPYFNE   +++   AE E  S +YN+ 
Sbjct: 753 NTWSGSGTEVWNAGSSSILQLLLSFQALVLNEKPYFNEAGYDKQLGRAEGEKNSVSYNEN 812

Query: 219 AYLFCCKMMMRLMKNPPKNFKEFVRNYFGEKGNKILAACKAYSDGRIRVGRFNEKENSES 278
           A+L  CK M+ +++ PPK+F+  V+++F  +   +LAACKAY +G + VG         S
Sbjct: 813 AFLITCKSMISMLRKPPKHFEMLVKDHFTHRAQHVLAACKAYMEG-VPVG--------SS 872

Query: 279 ESDYCNKADVSAIFKALMEKMHRDLHAAF 303
            +   N    S  FK ++ K++  L  AF
Sbjct: 873 ANLQGNSTTNSTGFKIMLSKLYPKLLEAF 892

[Close]

BLAST of Cla97C06G113960.1 vs. TAIR 10
Match: AT2G16920.1 (ubiquitin-conjugating enzyme 23 )

HSP 1 Score: 252.7 bits (644), Expect = 3.9e-67
Identity = 135/287 (47.04%), Postives = 178/287 (62.02%), Query Frame = 0


Query: 23   VDEPDRSPSPPPPSFSFKRFDIVTDYSDHHYADSNLFANCIGSDVS-----KKIMREWKI 82
            +++ D+       S SF+RFDI  D  DHH      F    G         KK+ ++WKI
Sbjct: 808  LEDEDKPVPSEGDSCSFRRFDISQDPLDHH------FLGVDGQKTKERQWFKKVDQDWKI 867

Query: 83   LERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFTSDYPISPPLVYYHSY 142
            L+ NLP+ IFVRAYE+R+DLLRAVIVG  GTPY DGLFFFD    SDYP  PP  YYHS 
Sbjct: 868  LQNNLPDGIFVRAYEDRMDLLRAVIVGAFGTPYQDGLFFFDFHLPSDYPSVPPSAYYHSG 927

Query: 143  GLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILISIQALVLNEKPYFNEPEL 202
            G RLNPNLYE+G VCLSL+NTW G+  E W+P  S++LQ+L+S+Q LVLN KPYFNE   
Sbjct: 928  GWRLNPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGY 987

Query: 203  EEE---AEAEIYSDAYNKEAYLFCCKMMMRLMKNPPKNFKEFVRNYFGEKGNKILAACKA 262
            +++   AE E  S  YN+  +L  CK MM LM+ PPK+F+E ++++F ++G  IL AC A
Sbjct: 988  DKQVGTAEGEKNSLGYNENTFLLNCKTMMYLMRKPPKDFEELIKDHFRKRGYYILKACDA 1047

Query: 263  YSDGRIRVGRFNEKENSESESDYCNKADVSAIFKALMEKMHRDLHAA 302
            Y  G + +G   +  +   E    N   V   FK ++ K+   L +A
Sbjct: 1048 YMKGYL-IGSLTKDASVIDERSSANSTSVG--FKLMLAKIAPKLFSA 1085

[Close]

BLAST of Cla97C06G113960.1 vs. TAIR 10
Match: AT1G53025.1 (Ubiquitin-conjugating enzyme family protein )

HSP 1 Score: 244.2 bits (622), Expect = 1.4e-64
Identity = 127/300 (42.33%), Postives = 175/300 (58.33%), Query Frame = 0


Query: 5   PLCYSHDGKDKHPATVDGVDEPDRSPSPPPPS----FSFKRFDIVTDYSDHHYADSNLFA 64
           P  Y       HP   + +     S +           FKRFD V D+SDHHYA     +
Sbjct: 206 PFSYDASASSSHPIAAEPISSVQDSSNARKLKEEFLRDFKRFDTVEDFSDHHYASKGKSS 265

Query: 65  NCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLA 124
                +  KK+  +WKILE +LPE+I VRA E R+DLLRAVI+G  GTPYHDGLFFFD+ 
Sbjct: 266 KQHSKNWVKKVQADWKILENDLPEAISVRACESRMDLLRAVIIGAEGTPYHDGLFFFDIQ 325

Query: 125 FTSDYPISPPLVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILIS 184
           F   YP  PP V+YHS GLR+NPNLY  G VCLSL+ TW G   E+W P  ST+LQ+L+S
Sbjct: 326 FPDTYPSVPPNVHYHSGGLRINPNLYNCGKVCLSLLGTWAGSAREKWLPNESTMLQLLVS 385

Query: 185 IQALVLNEKPYFNEPELEEE---AEAEIYSDAYNKEAYLFCCKMMMRLMKNPPKNFKEFV 244
           IQAL+LNEKPYFNEP   +    A  E  S  Y++  +L   K M+  ++ PP++F+E+V
Sbjct: 386 IQALILNEKPYFNEPGYVQSAGTASGESKSKVYSENVFLLSLKTMVYSIRRPPQHFEEYV 445

Query: 245 RNYFGEKGNKILAACKAYSD----GRIRVGRFNEKENSESESDYCNKADVSAIFKALMEK 294
           +N++  + + I+ AC AY      G +  G   + E +        K DV++  + ++++
Sbjct: 446 QNHYFVRSHDIVKACNAYKAGAPLGSMVKGGVQDLEEARQSGSKKFKTDVASFMQTVVDE 505

[Close]

BLAST of Cla97C06G113960.1 vs. TAIR 10
Match: AT1G53023.1 (Ubiquitin-conjugating enzyme family protein )

HSP 1 Score: 242.3 bits (617), Expect = 5.2e-64
Identity = 124/232 (53.45%), Postives = 155/232 (66.81%), Query Frame = 0


Query: 39  FKRFDIVTDYSDHHYADSNLFANCI---------GSDVSKKIMREWKILERNLPESIFVR 98
           FKRFD V D+SDHHYA     A              +  K I +EWKIL++NLPE+IFVR
Sbjct: 21  FKRFDTVEDFSDHHYAAEGSPAKLSLWHLKFKGRPKNWVKDIQKEWKILDKNLPETIFVR 80

Query: 99  AYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFTSDYPISPPLVYYHSYGLRLNPNLYEDG 158
           A E RIDLLRAVI+G  GTPYHDGLFFFD+ F   YP  PP V+YHS GLR+NPNLY+ G
Sbjct: 81  ACESRIDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPKVHYHSGGLRINPNLYKCG 140

Query: 159 YVCLSLINTWDGKEEERWNPAISTVLQILISIQALVLNEKPYFNEPELEEEAE---AEIY 218
            VCLSLI+TW GK+ E+W P  ST+LQ+L+SIQAL+LNEKPY+NEP  E+       E Y
Sbjct: 141 KVCLSLISTWTGKKREKWLPKESTMLQLLVSIQALILNEKPYYNEPGYEKSMGTPLGESY 200

Query: 219 SDAYNKEAYLFCCKMMMRLMKNPPKNFKEFVRNYFGEKGNKILAACKAYSDG 259
           S  Y++  ++F  K M         +F+EFVR++F  + + I+ AC AY DG
Sbjct: 201 SKDYSENVFVFSLKTM---------HFEEFVRSHFFVRSHDIVKACNAYKDG 243

The following BLAST results are available for this feature:

BLAST of Cla97C06G113960.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038907021.1	3.0e-162	91.50	probable ubiquitin-conjugating enzyme E2 25 [Benincasa hispida]	[more]
XP_008458344.1	6.0e-147	81.88	PREDICTED: putative ubiquitin-conjugating enzyme E2 39 [Cucumis melo] >TYK02958....	[more]
XP_004148800.1	2.3e-146	82.33	probable ubiquitin-conjugating enzyme E2 25 [Cucumis sativus] >KGN47459.1 hypoth...	[more]
XP_022930350.1	2.6e-134	81.13	probable ubiquitin-conjugating enzyme E2 25 [Cucurbita moschata]	[more]
XP_022999956.1	3.4e-134	81.13	probable ubiquitin-conjugating enzyme E2 25 [Cucurbita maxima]	[more]

BLAST of Cla97C06G113960.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LUQ5	5.2e-69	49.24	Probable ubiquitin-conjugating enzyme E2 25 OS=Arabidopsis thaliana OX=3702 GN=U...	[more]
Q8VY10	8.4e-67	47.96	Probable ubiquitin-conjugating enzyme E2 24 OS=Arabidopsis thaliana OX=3702 GN=U...	[more]
Q9ZVX1	5.4e-66	47.04	Probable ubiquitin-conjugating enzyme E2 23 OS=Arabidopsis thaliana OX=3702 GN=U...	[more]
Q9C918	6.6e-64	46.77	Putative ubiquitin-conjugating enzyme E2 38 OS=Arabidopsis thaliana OX=3702 GN=U...	[more]
Q8GY87	1.9e-63	42.33	Probable ubiquitin-conjugating enzyme E2 26 OS=Arabidopsis thaliana OX=3702 GN=U...	[more]

BLAST of Cla97C06G113960.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BVE0	2.9e-147	81.88	Putative ubiquitin-conjugating enzyme E2 39 OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A1S3C8W9	2.9e-147	81.88	putative ubiquitin-conjugating enzyme E2 39 OS=Cucumis melo OX=3656 GN=LOC103497...	[more]
A0A0A0KF71	1.1e-146	82.33	UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G330470 PE...	[more]
A0A6J1EWP5	1.3e-134	81.13	probable ubiquitin-conjugating enzyme E2 25 OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1KEJ5	1.7e-134	81.13	probable ubiquitin-conjugating enzyme E2 25 OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]

BLAST of Cla97C06G113960.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G15355.1	3.7e-70	49.24	ubiquitin-conjugating enzyme 25	[more]
AT2G33770.1	5.9e-68	47.96	phosphate 2	[more]
AT2G16920.1	3.9e-67	47.04	ubiquitin-conjugating enzyme 23	[more]
AT1G53025.1	1.4e-64	42.33	Ubiquitin-conjugating enzyme family protein	[more]
AT1G53023.1	5.2e-64	53.45	Ubiquitin-conjugating enzyme family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SMART	SM00212	ubc_7	coord: 69..223 e-value: 1.0E-23 score: 94.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 9..23
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..36
None	No IPR available	PANTHER	PTHR46116:SF19	UBIQUITIN-CONJUGATING ENZYME E2 38-RELATED	coord: 38..303
None	No IPR available	PANTHER	PTHR46116	(E3-INDEPENDENT) E2 UBIQUITIN-CONJUGATING ENZYME	coord: 38..303
IPR000608	Ubiquitin-conjugating enzyme E2	PFAM	PF00179	UQ_con	coord: 71..208 e-value: 1.6E-24 score: 86.1
IPR000608	Ubiquitin-conjugating enzyme E2	PROSITE	PS50127	UBC_2	coord: 66..223 score: 37.903706
IPR000608	Ubiquitin-conjugating enzyme E2	CDD	cd00195	UBCc	coord: 68..237 e-value: 1.39039E-38 score: 131.17
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	GENE3D	3.10.110.10	Ubiquitin Conjugating Enzyme	coord: 35..300 e-value: 1.8E-80 score: 272.3
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	SUPERFAMILY	54495	UBC-like	coord: 55..230

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cla97C06G113960	Cla97C06G113960	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cla97C06G113960.1-exon	Cla97C06G113960.1-exon-Cla97Chr06:4916144..4917070	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cla97C06G113960.1-cds	Cla97C06G113960.1-cds-Cla97Chr06:4916144..4917070	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cla97C06G113960.1	Cla97C06G113960.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0061631	ubiquitin conjugating enzyme activity