ClCG02G022260.2 (mRNA) Watermelon (Charleston Gray) v2.5

Overview


Name	ClCG02G022260.2
Type	mRNA
Organism	Citrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Description	DNA polymerase epsilon subunit 3
Location	CG_Chr02: 36706590 .. 36709387 (-)
Sequence length	1069
RNA-Seq Expression	ClCG02G022260.2
Synteny	ClCG02G022260.2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTAGTAAATATCACAAAAAGATGTGTTAATCCGGCCCGGCCCATTTTCCTGCCACAGGCACTAGCATCTAAAACGTCCAATAAAGCGAGGACGACATGTCGTCTTGTTTATCCAATCGGGTTTACGTAGATAACAGAAGATCAAGAAAAAAAGCCCACAAAACTGATCGGGCCGCCGTAACACGAAACTACCAGAACGATACTAATGGCGGGAAACAGACTCGAGGAGTCCGCCGGCACTCATTTACGCCGAAGAACCATAGAAGAAGAAGAAGAAGAACACATCGAGACAGCGTCAAGGGAGTAGAGAAATATGGAGAAAAAAGTAGGTGCCGGGATTGAAGAGCTTCCAAAGACTATAGTTCGCCGAGTGGTCAAAGAGAAGCTCTCTCAGTGCTCTCGGGACCAGGACATCTCCGTCAACAAAGACTCGCTTCTTGCCTTTTGCGAGAGCGCTCGAATCTTCATCCATTACCTCTCCGCTACGTAAGCTGCGTAACTTGCCCTAATGTGTTTAATTCCGAATGAAATTCTGTCCATTCTGATTAAAGCGATAAATCTATTGTTGTTGTGGCGCTAGGGTTAACGAAAATGTTGTTATGTAATAGTTAGTTCTTGCGTTCGTATTAACTGGTAGATCTCTAAGCAGGTCGAATGATATATGCAATGAATCAAAGAGGCAGACGATTAAGGCGGAGGATGTATTGAAAGCTCTAGAAGATATGGAATTTCCCGAATTGGTTAGGCCTCTTAAGGTCTCCCTCAATGGTGAGAAAGCTTCGCACCGCTTTGTTTACATTTTTTTAAAAAGAAAAAACTATGTCTTATTGGAGCCTGAAATTGCACGCTTAATTGGAGAACTATGTGTTGATAGTTTTCACTTGCAAAGCATTGTTCAAAAGGATGTTCTAAAACTTCTCTTCGACCTGCATTACAGTTGCGCATTAGTTAAACACATGAATCTTATTTGCGATGAAGTTCTCCATTTACCCTTTTTTTACTACTCGGTTGTTAGCATGGTCTTGTCTTTATGTGCTTGCTTGATTATCCTGCCTAAAAATGCCTTGGATTGCGAGACGTTAAACACTTCAAGAGTGTGAGGTATTGCACGGTTAGATGAAGCCCTATGCAAAGAGGACGTTGACATTATAAAACCAATCCTTATCAGTTCAAGCATACAAGATGAGTGAATTTAGCATTATGATAGGAATTGGACAAACATTTGTCAATGTGAAAAGTGGATACAAGTTGCAAATGAATATTTAATGCAAGTCTAGATGACTGCCGTTAGTTTCATTAGTTGAATTACGCTTGTAATATAAGAAGGCTGCATGAAATTCATCTCAGTTTCCTTAGTTTTACTTGATTGAATTTCGATTCTGAGAGAGCTGGTGAGAGCTCAAAGAACTTGTGCGTACTTCTATTAAAGGATTACGAAATAAATCTTTGAGTGCCTATGAATCCTCAGAACTTTTGGTTTGATGTCTTTTTATTTACTTGTATCCAGAAATGCTATCGAGAAATTGAGTTCTTTAAAACCTTGCTATATAGTGATGGGTTAGAGTTGGGATTTACTTTCTGGATTTCTTTTAGAAGTACATACTTATAGCGCTCCAAAGAGTATGTAAAGTGAGCAAGAATTTCTGTTTACTGGGGTGTAACAGAGGTTTTGTTTATTGAATTCTGGAATAGGGGTATTGCAATGTACTTTTAACTATTTCTAATTTCGTTTGTAGTTGAATATATGAGAATTGATATTGAGTCTGATCACACTTCACATTCTTGGATGGAGAGATACATTCTTAATGCATATATAGAAAGATTTATTAGTTCTTAATGCTGCAATGCAAGTTCAATGTGCAGATACTTTTCTTGTGTTATCTTTGTTGCTCGGTTTATTATGAGGCCAGAAAGGGTAATTGGTCATGTGACAGTATGCCTATTCTTGTTGGATAGTACATACAAGTATTATAATGTCAAAGCGGTTTAGTCTGAAATAGACGATTGCTGTGTTTAATTTAATGGAAAAGAAATGTGATATTCGGTTGCGCGCAGAAAGCAATATTAATCTCCACAGGCTATGATTTTCTATCTCATGGTCGCCACATTCATGTTCATACTTATCCAAGATCAATATTAAGATAAGCAGCTGAACTTTTTTAATGATTTATTCATTGTGCAAACCTTTGCTTGGCTGAATATAGAATTCAGACGCAAGAATGCCGGAAAGAAGGCAGCTGCGTCCAGAGAAAAAGAAGTGAAAAAGAAGAGGAAGGTAGAAGAGCCAACAGCTGAAGGTACAGATGGTGACAGCAATGATGGCGTCGGAAATGAGGATAATGATGAAGCCGAAGATGAAGATGAAATTGGCGCCAGTGATAACTGAGAAACACGAACTGTTTCACTAATTAGGTACTTGAAATGAGGTTACTCTACACTACTACGTTTATTTTCAAACTAGAAAACCCGATTTACGAGAAGGTTTACAAATCTTCTGGAAAGGAAGGTTTATTTAGGTTGTATATTGTGAAAGGCAGCTGCACATTTTAGCAAGTTTTGATCTCTTTGTAACCGTCTTTGTTAGAGAATTCCCCCCAATACTTAATAGTTCATTTGTTACTGCGTTTCTTTTTGTTATGTTGATGCAAAGACATTAAAAGGACATTTCTGTATCCTTGTTTGTTGCTTCAAATCCAGAATATGTTTAGTCGCATGCTATCAAAAACTTGCCGACCATGAAAATTTTCGATTGAAATCTTTGTTGTTTTATCGGGTAGTCACATATGTAATTATAG

mRNA sequence

ATGGTTAATAACAGAAGATCAAGAAAAAAAGCCCACAAAACTGATCGGGCCGCCGTAACACGAAACTACCAGAACGATACTAATGGCGGGAAACAGACTCGAGGAGTCCGCCGGCACTCATTTACGCCGAAGAACCATAGAAGAAGAAGAAGAAGAACACATCGAGACAGCGTCAAGGGAGTAGAGAAATATGGAGAAAAAAGTGCCGGGATTGAAGAGCTTCCAAAGACTATAGTTCGCCGAGTGGTCAAAGAGAAGCTCTCTCAGTGCTCTCGGGACCAGGACATCTCCGTCAACAAAGACTCGCTTCTTGCCTTTTGCGAGAGCGCTCGAATCTTCATCCATTACCTCTCCGCTACGTCGAATGATATATGCAATGAATCAAAGAGGCAGACGATTAAGGCGGAGGATGTATTGAAAGCTCTAGAAGATATGGAATTTCCCGAATTGGTTAGGCCTCTTAAGGTCTCCCTCAATGAATTCAGACGCAAGAATGCCGGAAAGAAGGCAGCTGCGTCCAGAGAAAAAGAAGTGAAAAAGAAGAGGAAGGTAGAAGAGCCAACAGCTGAAGGTACAGATGGTGACAGCAATGATGGCGTCGGAAATGAGGATAATGATGAAGCCGAAGATGAAGATGAAATTGGCGCCAGTGATAACTGAGAAACACGAACTGTTTCACTAATTAGGTACTTGAAATGAGGTTACTCTACACTACTACGTTTATTTTCAAACTAGAAAACCCGATTTACGAGAAGGTTTACAAATCTTCTGGAAAGGAAGGTTTATTTAGGTTGTATATTGTGAAAGGCAGCTGCACATTTTAGCAAGTTTTGATCTCTTTGTAACCGTCTTTGTTAGAGAATTCCCCCCAATACTTAATAGTTCATTTGTTACTGCGTTTCTTTTTGTTATGTTGATGCAAAGACATTAAAAGGACATTTCTGTATCCTTGTTTGTTGCTTCAAATCCAGAATATGTTTAGTCGCATGCTATCAAAAACTTGCCGACCATGAAAATTTTCGATTGAAATCTTTGTTGTTTTATCGGGTAGTCACATATGTAATTATAG

Coding sequence (CDS)

ATGGTTAATAACAGAAGATCAAGAAAAAAAGCCCACAAAACTGATCGGGCCGCCGTAACACGAAACTACCAGAACGATACTAATGGCGGGAAACAGACTCGAGGAGTCCGCCGGCACTCATTTACGCCGAAGAACCATAGAAGAAGAAGAAGAAGAACACATCGAGACAGCGTCAAGGGAGTAGAGAAATATGGAGAAAAAAGTGCCGGGATTGAAGAGCTTCCAAAGACTATAGTTCGCCGAGTGGTCAAAGAGAAGCTCTCTCAGTGCTCTCGGGACCAGGACATCTCCGTCAACAAAGACTCGCTTCTTGCCTTTTGCGAGAGCGCTCGAATCTTCATCCATTACCTCTCCGCTACGTCGAATGATATATGCAATGAATCAAAGAGGCAGACGATTAAGGCGGAGGATGTATTGAAAGCTCTAGAAGATATGGAATTTCCCGAATTGGTTAGGCCTCTTAAGGTCTCCCTCAATGAATTCAGACGCAAGAATGCCGGAAAGAAGGCAGCTGCGTCCAGAGAAAAAGAAGTGAAAAAGAAGAGGAAGGTAGAAGAGCCAACAGCTGAAGGTACAGATGGTGACAGCAATGATGGCGTCGGAAATGAGGATAATGATGAAGCCGAAGATGAAGATGAAATTGGCGCCAGTGATAACTGA

Protein sequence

MVNNRRSRKKAHKTDRAAVTRNYQNDTNGGKQTRGVRRHSFTPKNHRRRRRRTHRDSVKGVEKYGEKSAGIEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATSNDICNESKRQTIKAEDVLKALEDMEFPELVRPLKVSLNEFRRKNAGKKAAASREKEVKKKRKVEEPTAEGTDGDSNDGVGNEDNDEAEDEDEIGASDN

Homology

[Close]

BLAST of ClCG02G022260.2 vs. NCBI nr
Match: XP_038900596.1 (DNA polymerase epsilon subunit 3 [Benincasa hispida] >XP_038900597.1 DNA polymerase epsilon subunit 3 [Benincasa hispida])

HSP 1 Score: 249.2 bits (635), Expect = 3.3e-62
Identity = 137/151 (90.73%), Postives = 142/151 (94.04%), Query Frame = 0


Query: 69  AGIEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATSNDICNES 128
           A IEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATS DIC ES
Sbjct: 7   AAIEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATSYDICKES 66

Query: 129 KRQTIKAEDVLKALEDMEFPELVRPLKVSLNEFRRKNAGKKAAASREKEVKKKRKVEEPT 188
           KRQTIKAEDVLKALEDMEFPELVRPLK SLNEFRRKNAGKKAAASREKEVKKKRK+EEPT
Sbjct: 67  KRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRRKNAGKKAAASREKEVKKKRKIEEPT 126

Query: 189 AEGTDGDSNDGVGNEDNDEAEDEDEIGASDN 220
            EGTDG+S DGVGN+DNDE  +EDE GASD+
Sbjct: 127 VEGTDGESVDGVGNDDNDEDGNEDENGASDD 157

[Close]

BLAST of ClCG02G022260.2 vs. NCBI nr
Match: XP_008449581.1 (PREDICTED: DNA polymerase epsilon subunit D [Cucumis melo])

HSP 1 Score: 247.7 bits (631), Expect = 9.5e-62
Identity = 138/151 (91.39%), Postives = 143/151 (94.70%), Query Frame = 0


Query: 69  AGIEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATSNDICNES 128
           AGIEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSAT+NDIC ES
Sbjct: 7   AGIEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATANDICKES 66

Query: 129 KRQTIKAEDVLKALEDMEFPELVRPLKVSLNEFRRKNAGKKAAASREKEVKKKRKVEEPT 188
           KRQTIKAEDVLKALEDMEFPELVRPLK SLNEFRRKNAGKKAAA REKEVKKKRKVEEPT
Sbjct: 67  KRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRRKNAGKKAAALREKEVKKKRKVEEPT 126

Query: 189 AEGTDGDSNDGVGNEDNDEAEDEDEIGASDN 220
            EGTDG+S DGVG++DNDE EDE+E  ASDN
Sbjct: 127 VEGTDGESIDGVGDDDNDEDEDENE--ASDN 155

[Close]

BLAST of ClCG02G022260.2 vs. NCBI nr
Match: XP_004140162.1 (DNA polymerase epsilon subunit D [Cucumis sativus] >XP_031742448.1 DNA polymerase epsilon subunit D [Cucumis sativus] >KGN48033.1 hypothetical protein Csa_004222 [Cucumis sativus])

HSP 1 Score: 239.2 bits (609), Expect = 3.4e-59
Identity = 135/153 (88.24%), Postives = 140/153 (91.50%), Query Frame = 0


Query: 70  GIEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATSNDICNESK 129
           G EELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSAT+NDIC ESK
Sbjct: 8   GNEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATANDICKESK 67

Query: 130 RQTIKAEDVLKALEDMEFPELVRPLKVSLNEFRRKNAGKKAAASREKEVKKKRKVEEPTA 189
           RQTIKAEDVLKALEDMEFPELVRPLK SL+EFRRKNAGKKAAASREKE KKKRKVEEPT 
Sbjct: 68  RQTIKAEDVLKALEDMEFPELVRPLKASLDEFRRKNAGKKAAASREKEAKKKRKVEEPTV 127

Query: 190 EGTDGDSNDGVG---NEDNDEAEDEDEIGASDN 220
           EGTDG+S D VG   N+DNDE EDEDE  ASD+
Sbjct: 128 EGTDGESIDDVGNDDNDDNDEDEDEDENRASDD 160

[Close]

BLAST of ClCG02G022260.2 vs. NCBI nr
Match: XP_022152961.1 (DNA polymerase epsilon subunit 3 [Momordica charantia])

HSP 1 Score: 224.9 bits (572), Expect = 6.6e-55
Identity = 130/161 (80.75%), Postives = 138/161 (85.71%), Query Frame = 0


Query: 68  SAGIEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATSNDICNE 127
           +A IEELPKTIVRRVVKEKLSQCSR+QDISVNKDSLLAFCESARIFIHYLSATSNDIC E
Sbjct: 6   AAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSNDICKE 65

Query: 128 SKRQTIKAEDVLKALEDMEFPELVRPLKVSLNEFRRKNAGKKAAASREKEVKKKRKVEEP 187
           SKRQTIKA+DVLKALEDMEFPELVRPLK SLNEFRRK+AGKKAAASREKE KKKRK+EEP
Sbjct: 66  SKRQTIKADDVLKALEDMEFPELVRPLKASLNEFRRKSAGKKAAASREKEAKKKRKIEEP 125

Query: 188 TAEGTDGDSNDGVGNEDND----------EAEDEDEIGASD 219
           T E    +S D VGN+DND          E EDE+E GASD
Sbjct: 126 TVE---DESKDDVGNDDNDNDHDEDEDEVEDEDEEENGASD 163

[Close]

BLAST of ClCG02G022260.2 vs. NCBI nr
Match: KAG7035929.1 (DNA polymerase epsilon subunit 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 223.0 bits (567), Expect = 2.5e-54
Identity = 126/155 (81.29%), Postives = 135/155 (87.10%), Query Frame = 0


Query: 66  EKSAG--IEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATSND 125
           EK  G  IEELPKTIVRRVVKEKLSQCSR+QDISVNKDSLLAFCESARIFIHYLSATSND
Sbjct: 2   EKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSND 61

Query: 126 ICNESKRQTIKAEDVLKALEDMEFPELVRPLKVSLNEFRRKNAGKKAAASREKEVKKKRK 185
           IC ESKRQTIKAEDVLKALEDMEFPELVRPLK SLNEFR KNAGKKAAASR KE KK++ 
Sbjct: 62  ICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRKI 121

Query: 186 VEEPTAEGTDGDSNDGVGNEDNDEAEDEDEIGASD 219
            EEPT EGTDG+S D V N+++D  +DEDE+   D
Sbjct: 122 EEEPTVEGTDGESKDDVENDNDDNDKDEDEVEDED 156

[Close]

BLAST of ClCG02G022260.2 vs. ExPASy Swiss-Prot
Match: Q3SZN5 (DNA polymerase epsilon subunit 3 OS=Bos taurus OX=9913 GN=POLE3 PE=2 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.1e-12
Identity = 48/140 (34.29%), Postives = 81/140 (57.86%), Query Frame = 0


Query: 74  LPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATSNDICNESKRQTI 133
           LP  ++ R++KE L        ++++K++  A   +A +F+ Y ++ +N+   + KR+T+
Sbjct: 10  LPNAVITRIIKEAL-----PDGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTL 69

Query: 134 KAEDVLKALEDMEFPELVRPLKVSLNEFRRKNAGKKAAASREKEVKKKRKVEEPTAEGTD 193
            A DVL A+E+MEF   V PLK +L  +RR+  GKK A+ ++K+ K K+         TD
Sbjct: 70  NASDVLSAMEEMEFQRFVTPLKEALEAYRREQKGKKEASEQKKKDKDKK---------TD 129

Query: 194 GDSNDGVGNEDNDEAEDEDE 214
            +  D   +EDNDE E+  E
Sbjct: 130 SEEQDKSRDEDNDEDEERLE 135

[Close]

BLAST of ClCG02G022260.2 vs. ExPASy Swiss-Prot
Match: Q9NRF9 (DNA polymerase epsilon subunit 3 OS=Homo sapiens OX=9606 GN=POLE3 PE=1 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.1e-12
Identity = 48/140 (34.29%), Postives = 81/140 (57.86%), Query Frame = 0


Query: 74  LPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATSNDICNESKRQTI 133
           LP  ++ R++KE L        ++++K++  A   +A +F+ Y ++ +N+   + KR+T+
Sbjct: 10  LPNAVITRIIKEAL-----PDGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTL 69

Query: 134 KAEDVLKALEDMEFPELVRPLKVSLNEFRRKNAGKKAAASREKEVKKKRKVEEPTAEGTD 193
            A DVL A+E+MEF   V PLK +L  +RR+  GKK A+ ++K+ K K+         TD
Sbjct: 70  NASDVLSAMEEMEFQRFVTPLKEALEAYRREQKGKKEASEQKKKDKDKK---------TD 129

Query: 194 GDSNDGVGNEDNDEAEDEDE 214
            +  D   +EDNDE E+  E
Sbjct: 130 SEEQDKSRDEDNDEDEERLE 135

[Close]

BLAST of ClCG02G022260.2 vs. ExPASy Swiss-Prot
Match: Q5R4W3 (DNA polymerase epsilon subunit 3 OS=Pongo abelii OX=9601 GN=POLE3 PE=2 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.1e-12
Identity = 48/140 (34.29%), Postives = 81/140 (57.86%), Query Frame = 0


Query: 74  LPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATSNDICNESKRQTI 133
           LP  ++ R++KE L        ++++K++  A   +A +F+ Y ++ +N+   + KR+T+
Sbjct: 10  LPNAVITRIIKEAL-----PDGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTL 69

Query: 134 KAEDVLKALEDMEFPELVRPLKVSLNEFRRKNAGKKAAASREKEVKKKRKVEEPTAEGTD 193
            A DVL A+E+MEF   V PLK +L  +RR+  GKK A+ ++K+ K K+         TD
Sbjct: 70  NASDVLSAMEEMEFQRFVTPLKEALEAYRREQKGKKEASEQKKKDKDKK---------TD 129

Query: 194 GDSNDGVGNEDNDEAEDEDE 214
            +  D   +EDNDE E+  E
Sbjct: 130 SEEQDKSRDEDNDEDEERLE 135

[Close]

BLAST of ClCG02G022260.2 vs. ExPASy Swiss-Prot
Match: Q642A5 (DNA polymerase epsilon subunit 3 OS=Rattus norvegicus OX=10116 GN=Pole3 PE=1 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.2e-11
Identity = 45/141 (31.91%), Postives = 82/141 (58.16%), Query Frame = 0


Query: 74  LPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATSNDICNESKRQTI 133
           LP  ++ R++KE L        ++++K++  A   +A +F+ Y ++ +N+   + KR+T+
Sbjct: 10  LPNAVITRIIKEAL-----PDGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTL 69

Query: 134 KAEDVLKALEDMEFPELVRPLKVSLNEFRRKNAGKKAAASREKEVKKKRKVEEPTAEGTD 193
            A DVL A+E+MEF   V PLK +L  +RR+  GKK A+ ++K+ K K+  EE      +
Sbjct: 70  NASDVLSAMEEMEFQRFVTPLKEALEAYRREQKGKKEASEQKKKDKDKKDCEEQDKSREE 129

Query: 194 GDSNDGVGNEDNDEAEDEDEI 215
            D ++     D +E  +E+E+
Sbjct: 130 EDEDE--ERLDEEEQNEEEEV 143

[Close]

BLAST of ClCG02G022260.2 vs. ExPASy Swiss-Prot
Match: Q9JKP7 (DNA polymerase epsilon subunit 3 OS=Mus musculus OX=10090 GN=Pole3 PE=1 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.7e-11
Identity = 44/140 (31.43%), Postives = 80/140 (57.14%), Query Frame = 0


Query: 74  LPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATSNDICNESKRQTI 133
           LP  ++ R++KE L        ++++K++  A   +A +F+ Y ++ +N+   + KR+T+
Sbjct: 10  LPNAVITRIIKEAL-----PDGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTL 69

Query: 134 KAEDVLKALEDMEFPELVRPLKVSLNEFRRKNAGKKAAASREKEVKKKRKVEEPTAEGTD 193
            A DVL A+E+MEF   + PLK +L  +RR   GKK A+ ++K+ K K+  EE       
Sbjct: 70  NASDVLSAMEEMEFQRFITPLKEALEAYRRDEKGKKEASEQKKKDKDKKDSEEQ------ 129

Query: 194 GDSNDGVGNEDNDEAEDEDE 214
            D +  V  ED +  +++D+
Sbjct: 130 -DKSREVEEEDEERLDEDDQ 137

[Close]

BLAST of ClCG02G022260.2 vs. ExPASy TrEMBL
Match: A0A1S3BNA4 (DNA polymerase epsilon subunit D OS=Cucumis melo OX=3656 GN=LOC103491421 PE=4 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 4.6e-62
Identity = 138/151 (91.39%), Postives = 143/151 (94.70%), Query Frame = 0


Query: 69  AGIEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATSNDICNES 128
           AGIEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSAT+NDIC ES
Sbjct: 7   AGIEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATANDICKES 66

Query: 129 KRQTIKAEDVLKALEDMEFPELVRPLKVSLNEFRRKNAGKKAAASREKEVKKKRKVEEPT 188
           KRQTIKAEDVLKALEDMEFPELVRPLK SLNEFRRKNAGKKAAA REKEVKKKRKVEEPT
Sbjct: 67  KRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRRKNAGKKAAALREKEVKKKRKVEEPT 126

Query: 189 AEGTDGDSNDGVGNEDNDEAEDEDEIGASDN 220
            EGTDG+S DGVG++DNDE EDE+E  ASDN
Sbjct: 127 VEGTDGESIDGVGDDDNDEDEDENE--ASDN 155

[Close]

BLAST of ClCG02G022260.2 vs. ExPASy TrEMBL
Match: A0A0A0KEV7 (CBFD_NFYB_HMF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G425700 PE=4 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 1.6e-59
Identity = 135/153 (88.24%), Postives = 140/153 (91.50%), Query Frame = 0


Query: 70  GIEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATSNDICNESK 129
           G EELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSAT+NDIC ESK
Sbjct: 8   GNEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATANDICKESK 67

Query: 130 RQTIKAEDVLKALEDMEFPELVRPLKVSLNEFRRKNAGKKAAASREKEVKKKRKVEEPTA 189
           RQTIKAEDVLKALEDMEFPELVRPLK SL+EFRRKNAGKKAAASREKE KKKRKVEEPT 
Sbjct: 68  RQTIKAEDVLKALEDMEFPELVRPLKASLDEFRRKNAGKKAAASREKEAKKKRKVEEPTV 127

Query: 190 EGTDGDSNDGVG---NEDNDEAEDEDEIGASDN 220
           EGTDG+S D VG   N+DNDE EDEDE  ASD+
Sbjct: 128 EGTDGESIDDVGNDDNDDNDEDEDEDENRASDD 160

[Close]

BLAST of ClCG02G022260.2 vs. ExPASy TrEMBL
Match: A0A6J1DJA4 (DNA polymerase epsilon subunit 3 OS=Momordica charantia OX=3673 GN=LOC111020575 PE=4 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 3.2e-55
Identity = 130/161 (80.75%), Postives = 138/161 (85.71%), Query Frame = 0


Query: 68  SAGIEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATSNDICNE 127
           +A IEELPKTIVRRVVKEKLSQCSR+QDISVNKDSLLAFCESARIFIHYLSATSNDIC E
Sbjct: 6   AAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSNDICKE 65

Query: 128 SKRQTIKAEDVLKALEDMEFPELVRPLKVSLNEFRRKNAGKKAAASREKEVKKKRKVEEP 187
           SKRQTIKA+DVLKALEDMEFPELVRPLK SLNEFRRK+AGKKAAASREKE KKKRK+EEP
Sbjct: 66  SKRQTIKADDVLKALEDMEFPELVRPLKASLNEFRRKSAGKKAAASREKEAKKKRKIEEP 125

Query: 188 TAEGTDGDSNDGVGNEDND----------EAEDEDEIGASD 219
           T E    +S D VGN+DND          E EDE+E GASD
Sbjct: 126 TVE---DESKDDVGNDDNDNDHDEDEDEVEDEDEEENGASD 163

[Close]

BLAST of ClCG02G022260.2 vs. ExPASy TrEMBL
Match: A0A6J1H495 (DNA polymerase epsilon subunit 3 OS=Cucurbita moschata OX=3662 GN=LOC111459853 PE=4 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 6.0e-54
Identity = 130/164 (79.27%), Postives = 139/164 (84.76%), Query Frame = 0


Query: 66  EKSAG--IEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATSND 125
           EK  G  IEELPKTIVRRVVKEKLSQCSR+QDISVNKDSLLAFCESARIFIHYLSATSND
Sbjct: 2   EKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSND 61

Query: 126 ICNESKRQTIKAEDVLKALEDMEFPELVRPLKVSLNEFRRKNAGKKAAASREKEVKKKRK 185
           IC ESKRQTIKAEDVLKALEDMEFPELVRPLK SLNEFR KNAGKKAAASR KE KK++ 
Sbjct: 62  ICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRKI 121

Query: 186 VEEPTAEGTDGDSNDGVGNED------NDEAEDEDEI--GASDN 220
            EEPT EGTDG+S D V N++      ND+ EDEDE+  G SD+
Sbjct: 122 EEEPTVEGTDGESKDDVENDNDDNDDGNDKDEDEDEVENGGSDD 165

[Close]

BLAST of ClCG02G022260.2 vs. ExPASy TrEMBL
Match: A0A6J1K184 (DNA polymerase epsilon subunit 3 OS=Cucurbita maxima OX=3661 GN=LOC111490783 PE=4 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 6.0e-54
Identity = 127/156 (81.41%), Postives = 134/156 (85.90%), Query Frame = 0


Query: 66  EKSAG--IEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATSND 125
           EK  G  IEELPKTIVRRVVKEKLSQCSR+QDISVNKDSLLAFCESAR+FIHYLSATSND
Sbjct: 2   EKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARVFIHYLSATSND 61

Query: 126 ICNESKRQTIKAEDVLKALEDMEFPELVRPLKVSLNEFRRKNAGKKAAASREKEVKKKRK 185
           IC ESKRQTIKAEDVLKALEDMEFPELVRPLK SLNEFR KNAGKKAAASR KE KK++ 
Sbjct: 62  ICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRKI 121

Query: 186 VEEPTAEGTDGDSNDGVGN------EDNDEAEDEDE 214
            EEPT EGTDG+S D V N      +DND+ EDEDE
Sbjct: 122 EEEPTVEGTDGESKDDVENDNDDDDDDNDKDEDEDE 157

[Close]

BLAST of ClCG02G022260.2 vs. TAIR 10
Match: AT2G27470.1 (nuclear factor Y, subunit B11 )

HSP 1 Score: 136.3 bits (342), Expect = 2.9e-32
Identity = 82/174 (47.13%), Postives = 111/174 (63.79%), Query Frame = 0


Query: 71  IEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATSNDICNESKR 130
           ++ELP  IVRRVVK+KLS+CS D D+S++K++LLAF ESARIFIHYLSAT+ND C +++R
Sbjct: 8   VDELPLAIVRRVVKKKLSECSPDYDVSIHKEALLAFSESARIFIHYLSATANDFCKDARR 67

Query: 131 QTIKAEDVLKALEDMEFPELVRPLKVSLNEFRRKNAGKKA-------------------A 190
           QT+KA+DV KALE+M+F E + PLK SL +F++KNAGKKA                    
Sbjct: 68  QTMKADDVFKALEEMDFSEFLEPLKSSLEDFKKKNAGKKAGAAAASYPAGGAALKSSSGT 127

Query: 191 ASREKEVKK-------------KRKVEEPTAEGTDGDSNDGVGNEDNDEAEDED 213
           AS+ KE KK             K K++E T    +   ND    E+ ++ EDE+
Sbjct: 128 ASKPKETKKRKQEEPSTQKGARKSKIDEETKRNDEETENDNTEEENGNDEEDEN 181

[Close]

BLAST of ClCG02G022260.2 vs. TAIR 10
Match: AT5G23090.2 (nuclear factor Y, subunit B13 )

HSP 1 Score: 50.8 bits (120), Expect = 1.6e-06
Identity = 30/96 (31.25%), Postives = 51/96 (53.12%), Query Frame = 0


Query: 67  KSAGIEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATSNDICN 126
           KS     LPK  + +++KE L       D+ V +D+     E    FI+ +S+ SND+CN
Sbjct: 9   KSKEDASLPKATMTKIIKEML-----PPDVRVARDAQDLLIECCVEFINLVSSESNDVCN 68

Query: 127 ESKRQTIKAEDVLKALEDMEFPELVRPLKVSLNEFR 163
           +  ++TI  E VLKAL+ + F E +  +  +  + +
Sbjct: 69  KEDKRTIAPEHVLKALQVLGFGEYIEEVYAAYEQHK 99

[Close]

BLAST of ClCG02G022260.2 vs. TAIR 10
Match: AT5G23090.1 (nuclear factor Y, subunit B13 )

HSP 1 Score: 50.8 bits (120), Expect = 1.6e-06
Identity = 30/96 (31.25%), Postives = 51/96 (53.12%), Query Frame = 0


Query: 67  KSAGIEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATSNDICN 126
           KS     LPK  + +++KE L       D+ V +D+     E    FI+ +S+ SND+CN
Sbjct: 9   KSKEDASLPKATMTKIIKEML-----PPDVRVARDAQDLLIECCVEFINLVSSESNDVCN 68

Query: 127 ESKRQTIKAEDVLKALEDMEFPELVRPLKVSLNEFR 163
           +  ++TI  E VLKAL+ + F E +  +  +  + +
Sbjct: 69  KEDKRTIAPEHVLKALQVLGFGEYIEEVYAAYEQHK 99

[Close]

BLAST of ClCG02G022260.2 vs. TAIR 10
Match: AT5G23090.4 (nuclear factor Y, subunit B13 )

HSP 1 Score: 50.8 bits (120), Expect = 1.6e-06
Identity = 30/96 (31.25%), Postives = 51/96 (53.12%), Query Frame = 0


Query: 67  KSAGIEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATSNDICN 126
           KS     LPK  + +++KE L       D+ V +D+     E    FI+ +S+ SND+CN
Sbjct: 9   KSKEDASLPKATMTKIIKEML-----PPDVRVARDAQDLLIECCVEFINLVSSESNDVCN 68

Query: 127 ESKRQTIKAEDVLKALEDMEFPELVRPLKVSLNEFR 163
           +  ++TI  E VLKAL+ + F E +  +  +  + +
Sbjct: 69  KEDKRTIAPEHVLKALQVLGFGEYIEEVYAAYEQHK 99

[Close]

BLAST of ClCG02G022260.2 vs. TAIR 10
Match: AT5G08190.1 (nuclear factor Y, subunit B12 )

HSP 1 Score: 50.1 bits (118), Expect = 2.7e-06
Identity = 30/96 (31.25%), Postives = 51/96 (53.12%), Query Frame = 0


Query: 67  KSAGIEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATSNDICN 126
           KS     LPK  + +++KE L       D+ V +D+     E    FI+ +S+ SN++CN
Sbjct: 9   KSKEDASLPKATMTKIIKEML-----PADVRVARDAQDLLIECCVEFINLISSESNEVCN 68

Query: 127 ESKRQTIKAEDVLKALEDMEFPELVRPLKVSLNEFR 163
           +  ++TI  E VLKAL+ + F E V  +  +  + +
Sbjct: 69  KEDKRTIAPEHVLKALQVLGFGEYVEEVYAAYEQHK 99

The following BLAST results are available for this feature:

BLAST of ClCG02G022260.2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038900596.1	3.3e-62	90.73	DNA polymerase epsilon subunit 3 [Benincasa hispida] >XP_038900597.1 DNA polymer...	[more]
XP_008449581.1	9.5e-62	91.39	PREDICTED: DNA polymerase epsilon subunit D [Cucumis melo]	[more]
XP_004140162.1	3.4e-59	88.24	DNA polymerase epsilon subunit D [Cucumis sativus] >XP_031742448.1 DNA polymeras...	[more]
XP_022152961.1	6.6e-55	80.75	DNA polymerase epsilon subunit 3 [Momordica charantia]	[more]
KAG7035929.1	2.5e-54	81.29	DNA polymerase epsilon subunit 3 [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of ClCG02G022260.2 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q3SZN5	1.1e-12	34.29	DNA polymerase epsilon subunit 3 OS=Bos taurus OX=9913 GN=POLE3 PE=2 SV=1	[more]
Q9NRF9	1.1e-12	34.29	DNA polymerase epsilon subunit 3 OS=Homo sapiens OX=9606 GN=POLE3 PE=1 SV=1	[more]
Q5R4W3	1.1e-12	34.29	DNA polymerase epsilon subunit 3 OS=Pongo abelii OX=9601 GN=POLE3 PE=2 SV=1	[more]
Q642A5	1.2e-11	31.91	DNA polymerase epsilon subunit 3 OS=Rattus norvegicus OX=10116 GN=Pole3 PE=1 SV=...	[more]
Q9JKP7	2.7e-11	31.43	DNA polymerase epsilon subunit 3 OS=Mus musculus OX=10090 GN=Pole3 PE=1 SV=1	[more]

BLAST of ClCG02G022260.2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BNA4	4.6e-62	91.39	DNA polymerase epsilon subunit D OS=Cucumis melo OX=3656 GN=LOC103491421 PE=4 SV...	[more]
A0A0A0KEV7	1.6e-59	88.24	CBFD_NFYB_HMF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G4257...	[more]
A0A6J1DJA4	3.2e-55	80.75	DNA polymerase epsilon subunit 3 OS=Momordica charantia OX=3673 GN=LOC111020575 ...	[more]
A0A6J1H495	6.0e-54	79.27	DNA polymerase epsilon subunit 3 OS=Cucurbita moschata OX=3662 GN=LOC111459853 P...	[more]
A0A6J1K184	6.0e-54	81.41	DNA polymerase epsilon subunit 3 OS=Cucurbita maxima OX=3661 GN=LOC111490783 PE=...	[more]

BLAST of ClCG02G022260.2 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G27470.1	2.9e-32	47.13	nuclear factor Y, subunit B11	[more]
AT5G23090.2	1.6e-06	31.25	nuclear factor Y, subunit B13	[more]
AT5G23090.1	1.6e-06	31.25	nuclear factor Y, subunit B13	[more]
AT5G23090.4	1.6e-06	31.25	nuclear factor Y, subunit B13	[more]
AT5G08190.1	2.7e-06	31.25	nuclear factor Y, subunit B12	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009072	Histone-fold	GENE3D	1.10.20.10	Histone, subunit A	coord: 63..215 e-value: 1.0E-32 score: 114.8
IPR009072	Histone-fold	SUPERFAMILY	47113	Histone-fold	coord: 74..183
IPR003958	Transcription factor CBF/NF-Y/archaeal histone domain	PFAM	PF00808	CBFD_NFYB_HMF	coord: 73..142 e-value: 1.1E-8 score: 35.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 164..219
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 37..56
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 18..35
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 164..190
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..64
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..15
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 197..219
None	No IPR available	PANTHER	PTHR46172	DNA POLYMERASE EPSILON SUBUNIT 3	coord: 69..211

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
ClCG02G022260	ClCG02G022260	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
ClCG02G022260.2-exon	ClCG02G022260.2-exon-CG_Chr02:36706590..36707180	exon
ClCG02G022260.2-exon	ClCG02G022260.2-exon-CG_Chr02:36708617..36708735	exon
ClCG02G022260.2-exon	ClCG02G022260.2-exon-CG_Chr02:36708899..36709055	exon
ClCG02G022260.2-exon	ClCG02G022260.2-exon-CG_Chr02:36709060..36709254	exon
ClCG02G022260.2-exon	ClCG02G022260.2-exon-CG_Chr02:36709381..36709387	exon

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
ClCG02G022260.2-three_prime_utr	ClCG02G022260.2-three_prime_utr-CG_Chr02:36706590..36706998	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
ClCG02G022260.2-cds	ClCG02G022260.2-cds-CG_Chr02:36706999..36707180	CDS
ClCG02G022260.2-cds	ClCG02G022260.2-cds-CG_Chr02:36708617..36708735	CDS
ClCG02G022260.2-cds	ClCG02G022260.2-cds-CG_Chr02:36708899..36709055	CDS
ClCG02G022260.2-cds	ClCG02G022260.2-cds-CG_Chr02:36709060..36709254	CDS
ClCG02G022260.2-cds	ClCG02G022260.2-cds-CG_Chr02:36709381..36709387	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
ClCG02G022260.2	ClCG02G022260.2-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006974	cellular response to DNA damage stimulus
biological_process	GO:0031507	heterochromatin assembly
biological_process	GO:0006272	leading strand elongation
biological_process	GO:0042766	nucleosome mobilization
cellular_component	GO:0008623	CHRAC
cellular_component	GO:0008622	epsilon DNA polymerase complex
molecular_function	GO:0046982	protein heterodimerization activity
molecular_function	GO:0031490	chromatin DNA binding