Chy8G151830.1 (mRNA) Cucumber (hystrix) v1

BLAST of Chy8G151830.1 vs. ExPASy Swiss-Prot
Match: Q9C7N2 (MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=2 SV=1)

HSP 1 Score: 694.5 bits (1791), Expect = 1.0e-198
Identity = 340/578 (58.82%), Postives = 426/578 (73.70%), Query Frame = 0

Query: 10  TASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVDVDDEHSRDICLYDDVVLHNVSWDIKN 69
           T  NA+QALGRGFDV  D RLLYCKG  GS +V +++  +RD+ L    +L NV  DI  
Sbjct: 20  TLRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPNVPADIDC 79

Query: 70  FQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNCAFGFTGLKQTDASATKSLSTD 129
            +   G +   +CSF EMA+ FN + G+    P G FN  F +TG  Q DA++TKSL+  
Sbjct: 80  SRGNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALV 139

Query: 130 GFYIPLAKFQFTKTPLELRENVKRTVPTTWDPPSLASFIENFGTHVITSITIGGKDVIYV 189
           G++IPL   +  K  L L   ++R VP++WDP SLASFIEN+GTH++TS+TIGG+DV+Y+
Sbjct: 140 GYFIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYI 199

Query: 190 KQHQSSPLSTMEVKRYVQDIANQRFSDTQGQMGSGSIKLKEKGLEGGLFNNQGVYPQPTS 249
           +QHQSSPL   E++ YV D+   RF + + Q  +G +K K+K                  
Sbjct: 200 RQHQSSPLPVSEIENYVNDMIKHRFHEAESQSITGPLKYKDK------------------ 259

Query: 250 APYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALLDGVAGKEHL 309
                   D+TVIFRRRGGDDLEQ+H RWA TV ++PD+I MTF PI +LL+GV G  HL
Sbjct: 260 --------DITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHL 319

Query: 310 TRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKIPGLQRKEPVCPSLQFSIMGQKLYV 369
           TRAI LYLEYKPP+E+L+YFL++QI R WAP   +   LQRKEPVC SLQFS+MG KL++
Sbjct: 320 TRAIELYLEYKPPIEDLQYFLDYQIARAWAP---EQSNLQRKEPVCSSLQFSLMGPKLFI 379

Query: 370 SIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDAHVAIGAPKWQGPE 429
           S +Q+++GRKPVTG+RLSLEGSKQNRLSIHLQHL SLPKI+ P+WD+HV IGAPKWQGPE
Sbjct: 380 SADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWDSHVPIGAPKWQGPE 439

Query: 430 EQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGSRNVLYMKLL 489
           EQDSRWFEP+KWKNFSHVST+PIE+ ET +G+LSGV+IVTGAQLGVWDFGS+NVL++KLL
Sbjct: 440 EQDSRWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLKLL 499

Query: 490 YSRLPGCTIRRTLWDHLPQIERSKKLVSSSDNTNNADNSSSDTKNTQG-SNKLAKLVDTT 549
           +S++PGCTIRR++WDH P       + SS        ++SS T+   G S KLAK+VD++
Sbjct: 500 FSKVPGCTIRRSVWDHTP-------VASSGRLEPGGPSTSSSTEEVSGQSGKLAKIVDSS 559

Query: 550 EMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           EM KGPQD PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 560 EMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 561

BLAST of Chy8G151830.1 vs. ExPASy Swiss-Prot
Match: Q9STW5 (MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4g24290 PE=2 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 4.0e-118
Identity = 254/607 (41.85%), Postives = 363/607 (59.80%), Query Frame = 0

Query: 7   ALHTASNAVQALGRGFDVNFDTRLLYCKGVA-GSTIVDV-DDEHSRDICLYDDVVLHNVS 66
           A   A  A+ ++G G+D+  D RL YCKG +  S ++D+ + + + +I L   + + NVS
Sbjct: 7   ASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVS 66

Query: 67  WDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNCAFGFTGLKQTDASATK 126
             IK  +       S I  F +MA+ FNQ+  L+   PSG FN  F F+   Q DA+ TK
Sbjct: 67  KSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTK 126

Query: 127 SLSTDGFYIPLAKFQFTKTPLELRENVKRTVPTTWDPPSLASFIENFGTHVITSITIGGK 186
           +L+ DG +I L      K+ + LRE+VK+ VP+TWDP +LA FI+ +GTH+I S+ +GGK
Sbjct: 127 NLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGK 186

Query: 187 DVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQMGSGS--------IKLKEKGLEGG 246
           DVIY KQ  SS L   ++++ ++++A++RF +      +GS        ++ KE+ L   
Sbjct: 187 DVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFA 246

Query: 247 LFNNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQ-NHVRWARTVRSSPDVIEMTFNP 306
             ++ G Y           KED   + +RRGG+D     H  W +TV+  PDVI M+F P
Sbjct: 247 DTSSLGSY---------ANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIP 306

Query: 307 ITALLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKIP-GLQRKEPV 366
           IT+LL+GV G   L+ AI LYL YKPP+EEL  FLEFQ+PR WAP+  ++P G QRK+  
Sbjct: 307 ITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQS 366

Query: 367 CPSLQFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYW 426
           C SLQFS  G KLYV+   + +G++P+TGMRL LEG + NRL+IHLQHL+SLPKI     
Sbjct: 367 CASLQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLED 426

Query: 427 DAHVAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLG 486
           D + +I        E  D R++E V WKN+SHV T P+E+ +    +LS   +VTGAQL 
Sbjct: 427 DLNRSI------RQESHDRRYYEKVNWKNYSHVCTEPVESDD----DLS---VVTGAQLH 486

Query: 487 VWDFGSRNVLYMKLLYSRLPGCT-IRRTLWDHLPQIERSKKLVS---------------- 546
           V   G +NVL+++L +SR+ G T ++ + WD          L+S                
Sbjct: 487 VESHGFKNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPR 546

Query: 547 -SSDNTNNADNSSSDTKNTQGSNKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIV 584
            +  N N+A         TQ + KL K VDT+EM++GPQ+SPG+W+V+GA+L VEKGKI 
Sbjct: 547 PADVNINSAIYPGGPPVPTQ-APKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKIS 590

BLAST of Chy8G151830.1 vs. ExPASy Swiss-Prot
Match: Q9SGN6 (MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=2 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 4.2e-107
Identity = 238/602 (39.53%), Postives = 345/602 (57.31%), Query Frame = 0

Query: 11  ASNAVQALGRGFDVNFDTRLLYCKGVA-GSTIVDVDDEHSRDICLYDDVVLHNVSWDIKN 70
           A  AV  +G G+D+  D R   CK    GS +V++D   +RD+     +V++NVS  IK 
Sbjct: 15  AEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIKC 74

Query: 71  FQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNCAFGFTGLKQTDASATKSLSTD 130
            +       S I SF++M++ FNQ   LS   PSG FN  F F+     DAS+ K+L+ D
Sbjct: 75  DKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLAYD 134

Query: 131 GFYIPLAKFQFTKTPLELRENVKRTVPTTWDPPSLASFIENFGTHVITSITIGGKDVIYV 190
           G++I L   +  +  L LR+ VKR VP++WD  +LA FIE +GTHV+  +T+GGKDVI+V
Sbjct: 135 GWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIHV 194

Query: 191 KQHQSSPLSTMEVKRYVQDIANQRF------SDTQGQMGSGSIKLKEKGLEGGLFNNQGV 250
           KQ + S     E+++ ++   ++RF      S +   + SG  K +E  L+ GL   Q  
Sbjct: 195 KQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPK-EENLLQWGL---QPF 254

Query: 251 YPQPTSAPYL-TGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALLDG 310
               +SA  + T  E++  +  RRGG DL Q+H RW  TV  +P+VI M F PIT+LL G
Sbjct: 255 GTSVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSLLSG 314

Query: 311 VAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKIP-GLQRKEPVCPSLQFS 370
           + G   L+ A+ LYL YKPP+EEL  FLEFQ+PR WAP++  +P GL+R +   PSLQFS
Sbjct: 315 LPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQFS 374

Query: 371 IMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMH-PYWDAHVAI 430
           +MG KLYV+  ++  G +PVTG+R  LEG K N L+IHLQHL++ P  +H  + D +  I
Sbjct: 375 LMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEPI 434

Query: 431 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 490
             P  +G       ++ PVKW  FSHV T P++       + +   IVT A L V   G 
Sbjct: 435 EEPVEKG-------YYVPVKWGIFSHVCTYPVQYNGARSDDTAS--IVTKAWLEVKGMGM 494

Query: 491 RNVLYMKLLYSRLPGCTIRRTLWDHLPQIERSK---KLVSSSDNTNNADNSSSDTKNTQ- 550
           R VL+++L +S       R++ WD+L    R      ++S+  +T  + N ++    ++ 
Sbjct: 495 RKVLFLRLGFSLDASAVTRKSCWDNLSTNSRKSGVFSMISTRLSTGLSPNPATTKPQSKI 554

Query: 551 --------------GSNKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYS 585
                            KL  LVDT E+ +GP++ PG+W+VTGAKL VE GKI ++ KYS
Sbjct: 555 DINSAVYPRGPSPPVKPKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYS 603

BLAST of Chy8G151830.1 vs. ExPASy Swiss-Prot
Match: Q8L612 (MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1g14780 PE=2 SV=1)

HSP 1 Score: 359.8 bits (922), Expect = 6.0e-98
Identity = 223/623 (35.79%), Postives = 333/623 (53.45%), Query Frame = 0

Query: 14  AVQALGRGFDVNFDTRLLYCKGVAGST----IVDVDDEHSRDICLYDDVVLHNVSWDIKN 73
           AV++LG+GFD+  D RL YCK   GS     +V +D   +R++ +    V  NVS DI  
Sbjct: 12  AVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADINC 71

Query: 74  FQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNCAFGF-TGLKQTDASATKSLST 133
            +       S I  F++M++YFNQ+  ++   PSG+FN  FGF +G   TDA+  KSL  
Sbjct: 72  DKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSLGL 131

Query: 134 DGFYIPLAKFQF-TKTPLELRENVKRTVPTTWDPPSLASFIENFGTHVITSITIGGKDVI 193
           D   + L          L L + V+  VP++WDP  LA FIE +GTHVIT +++GG+DV+
Sbjct: 132 DASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQDVV 191

Query: 194 YVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQMGSGSIKLKEKGLEGGLFNNQ------ 253
            V+Q +SS L    ++ ++ D+ +Q F        +GS  L  + L     ++       
Sbjct: 192 VVRQDKSSDLDNDLLRHHLYDLGDQLF--------TGSCLLSTRRLNKAYHHSHSQPKFP 251

Query: 254 ---GVYPQPTSAPY----LTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFN 313
               V+    +  +    +  +  +TVI  +RGGD   ++H  W  TV   PD I   F 
Sbjct: 252 EAFNVFDDKQTVAFNNFSINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFI 311

Query: 314 PITALLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKIP--GLQRKE 373
           PIT+LL  V G   L+ A+ LYL YKPPL +L+YFL+F  PR WAP+H+ +P        
Sbjct: 312 PITSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMA 371

Query: 374 PVCPSLQFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASL-PKIMH 433
              P+L  + MG KLYV+   ++  + PVTGMR  LEG K NRL+IHLQHL +    +  
Sbjct: 372 SAYPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGE 431

Query: 434 PYWDAHVAIGAPKWQGPEE--QDSRWFEPVKWKNFSHVSTAPIENPETFMGELSG----- 493
              D H+      W+G ++   + R+FEP+  K FSHV T P++    ++   S      
Sbjct: 432 KITDEHI------WRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQN 491

Query: 494 --VYIVTGAQLGVWDFGSRNVLYMKLLYSRLPGCTIRRTLWDHLPQIERSKK-------- 553
              +IVTGAQL V   GS++VL+++L Y+++    + +  W H P I  S+K        
Sbjct: 492 DVAFIVTGAQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGP-IGTSQKSGIFSSMS 551

Query: 554 --LVSSSDNTNNADNSSSDTKNTQG----------SNKLAKLVDTTEMSKGPQDSPGHWL 586
             L S S + N      ++     G          +NK+ K VD +++ +GPQ SPGHWL
Sbjct: 552 MPLTSGSVHHNMIQKDKNEVVLDSGVFPGGPPVPANNKIVKFVDLSQLCRGPQHSPGHWL 611

BLAST of Chy8G151830.1 vs. ExPASy TrEMBL
Match: A0A0A0L2V7 (MACPF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G332620 PE=4 SV=1)

HSP 1 Score: 1171.0 bits (3028), Expect = 0.0e+00
Identity = 571/586 (97.44%), Postives = 578/586 (98.63%), Query Frame = 0

Query: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVDVDDEHSRDICLYDDVVL 60
           MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIV+VDDEH+RDICLYDDVVL
Sbjct: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNCAFGFTGLKQTDA 120
           HNV WDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFN  FGFTGLKQTDA
Sbjct: 61  HNVPWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSVFGFTGLKQTDA 120

Query: 121 SATKSLSTDGFYIPLAKFQFTKTPLELRENVKRTVPTTWDPPSLASFIENFGTHVITSIT 180
           SATKSLSTDGFYIPLAKFQFTKTPLEL+ENVKR VPTTWDPPSLASFIENFGTHVITSIT
Sbjct: 121 SATKSLSTDGFYIPLAKFQFTKTPLELKENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQMGSGSIKLKEKGLEGGLFNN 240
           IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQ GSGSIKLKEKGLEGGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLEGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALL 300
           QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKIPGLQRKEPVCPSLQF 360
           DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKIPG QRKEPVCPSLQF
Sbjct: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKIPGRQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDAHVAI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIM PYWDAH+AI
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMVPYWDAHIAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVY+VTGAQLGVWDFGS
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYVVTGAQLGVWDFGS 480

Query: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPQIERSKKLVSSSDNTNNADNSSSDTKNTQGSNK 540
           RNVLYMKLLYSRLPGCTIRRTLWDHLP IE+SKKLVSSSDNTNNADNSSSDTKNTQG+NK
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSDTKNTQGNNK 540

Query: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 586

BLAST of Chy8G151830.1 vs. ExPASy TrEMBL
Match: A0A5D3CHB3 (MACPF domain-containing protein CAD1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold127G00610 PE=4 SV=1)

HSP 1 Score: 1166.4 bits (3016), Expect = 0.0e+00
Identity = 568/586 (96.93%), Postives = 577/586 (98.46%), Query Frame = 0

Query: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVDVDDEHSRDICLYDDVVL 60
           MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIV+VDDEHSRDICLYDDVVL
Sbjct: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHSRDICLYDDVVL 60

Query: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNCAFGFTGLKQTDA 120
           HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFN AFGFTGL+QTDA
Sbjct: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSAFGFTGLRQTDA 120

Query: 121 SATKSLSTDGFYIPLAKFQFTKTPLELRENVKRTVPTTWDPPSLASFIENFGTHVITSIT 180
           SATKSLSTDGFYIPLAKFQFTKTPLEL+EN+KR VPTTWDPPSLASFIENFGTHVITSIT
Sbjct: 121 SATKSLSTDGFYIPLAKFQFTKTPLELKENIKRAVPTTWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQMGSGSIKLKEKGLEGGLFNN 240
           IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQ GSGSIKLKEKGL+GGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALL 300
           QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTF PITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKIPGLQRKEPVCPSLQF 360
           DGV GKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDK+PG QRKEPVCPSLQF
Sbjct: 301 DGVVGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKLPGRQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDAHVAI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWD HVAI
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480

Query: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPQIERSKKLVSSSDNTNNADNSSSDTKNTQGSNK 540
           RNVLYMKLLYSRLPGCTIRRTLWDHLP IE+SKKLVSSSDNTNNADNSSS TK+TQG+NK
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSGTKSTQGNNK 540

Query: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 586

BLAST of Chy8G151830.1 vs. ExPASy TrEMBL
Match: A0A1S3CJ73 (MACPF domain-containing protein CAD1-like OS=Cucumis melo OX=3656 GN=LOC103501463 PE=4 SV=1)

HSP 1 Score: 1166.4 bits (3016), Expect = 0.0e+00
Identity = 568/586 (96.93%), Postives = 577/586 (98.46%), Query Frame = 0

Query: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVDVDDEHSRDICLYDDVVL 60
           MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIV+VDDEHSRDICLYDDVVL
Sbjct: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHSRDICLYDDVVL 60

Query: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNCAFGFTGLKQTDA 120
           HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFN AFGFTGL+QTDA
Sbjct: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSAFGFTGLRQTDA 120

Query: 121 SATKSLSTDGFYIPLAKFQFTKTPLELRENVKRTVPTTWDPPSLASFIENFGTHVITSIT 180
           SATKSLSTDGFYIPLAKFQFTKTPLEL+EN+KR VPTTWDPPSLASFIENFGTHVITSIT
Sbjct: 121 SATKSLSTDGFYIPLAKFQFTKTPLELKENIKRAVPTTWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQMGSGSIKLKEKGLEGGLFNN 240
           IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQ GSGSIKLKEKGL+GGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALL 300
           QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTF PITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKIPGLQRKEPVCPSLQF 360
           DGV GKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDK+PG QRKEPVCPSLQF
Sbjct: 301 DGVVGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKLPGRQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDAHVAI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWD HVAI
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480

Query: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPQIERSKKLVSSSDNTNNADNSSSDTKNTQGSNK 540
           RNVLYMKLLYSRLPGCTIRRTLWDHLP IE+SKKLVSSSDNTNNADNSSS TK+TQG+NK
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSGTKSTQGNNK 540

Query: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 586

BLAST of Chy8G151830.1 vs. ExPASy TrEMBL
Match: A0A6J1CR77 (MACPF domain-containing protein CAD1-like OS=Momordica charantia OX=3673 GN=LOC111013848 PE=4 SV=1)

HSP 1 Score: 1077.8 bits (2786), Expect = 0.0e+00
Identity = 530/586 (90.44%), Postives = 553/586 (94.37%), Query Frame = 0

Query: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVDVDDEHSRDICLYDDVVL 60
           MG++GTALHTA NAVQALGRGFDVNFDTRLLYCKGV+GSTIV+VDDEH+RDICLYDDVVL
Sbjct: 1   MGDTGTALHTAINAVQALGRGFDVNFDTRLLYCKGVSGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNCAFGFTGLKQTDA 120
            NVSWDIKNFQEPIGHRSSGICSFDEM +YFNQKDGLSEGY SGSFN AFGF G KQ DA
Sbjct: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGFNGSKQIDA 120

Query: 121 SATKSLSTDGFYIPLAKFQFTKTPLELRENVKRTVPTTWDPPSLASFIENFGTHVITSIT 180
           + TK+LS DGFYIPLAKFQFTKTPL L+ENVKR +PT+WDPPSLASFIENFGTHVITSIT
Sbjct: 121 ATTKNLSMDGFYIPLAKFQFTKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQMGSGSIKLKEKGLEGGLFNN 240
           IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQG  GSGSIKLKEKGL+GGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGLTGSGSIKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALL 300
           QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVR SPDVIEMTF PITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRLSPDVIEMTFFPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKIPGLQRKEPVCPSLQF 360
           DGV GKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAP+ D +PG QRKEPVCPSLQF
Sbjct: 301 DGVTGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTVPGHQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDAHVAI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKI+ PYWD HV I
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHVGI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFG 
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGP 480

Query: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPQIERSKKLVSSSDNTNNADNSSSDTKNTQGSNK 540
           RNVLYMKLLYSRLPGCTI+RTLWDHL   ++SKKLVSSSDN +NAD+SSS ++N QG NK
Sbjct: 481 RNVLYMKLLYSRLPGCTIQRTLWDHLLN-DKSKKLVSSSDN-SNADDSSSGSRNIQG-NK 540

Query: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           LAKLVDT+EMSKGP D PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKLVDTSEMSKGPHDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 583

BLAST of Chy8G151830.1 vs. ExPASy TrEMBL
Match: A0A6J1EBB6 (MACPF domain-containing protein CAD1-like OS=Cucurbita moschata OX=3662 GN=LOC111432562 PE=4 SV=1)

HSP 1 Score: 1073.2 bits (2774), Expect = 4.4e-310
Identity = 530/587 (90.29%), Postives = 552/587 (94.04%), Query Frame = 0

Query: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVDVDDEHSRDICLYDDVVL 60
           M ESGTALHTA NAVQALGRGFDVNFDTRLLYCKGVAGSTIV+VDDEH+RDICLYDDVVL
Sbjct: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNCAFGFTGLKQTDA 120
            NVSWDIKNFQ+PIGHRSSGICSFDEM DYFNQKDGLSEGYPSGSFN AFGFTG KQ DA
Sbjct: 61  LNVSWDIKNFQQPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNTAFGFTGSKQIDA 120

Query: 121 SATKSLSTDGFYIPLAKFQFTKTPLELRENVKRTVPTTWDPPSLASFIENFGTHVITSIT 180
           +A K+LSTDGFYIPLAKFQ T T L L ENVKR+VPT+WDPP+LASFIENFGTHVITSIT
Sbjct: 121 AAAKNLSTDGFYIPLAKFQLTSTSLLLHENVKRSVPTSWDPPALASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQMGSGSIKLKEKGLEGGLFNN 240
           IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQ GSGS KLKEKGL+GGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQAGSGSRKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALL 300
           QGVYPQPTSAPY +GKEDVTVIFRRRGGDDLEQNHV+WA+TVRSSPDVIEMTF PITALL
Sbjct: 241 QGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLEQNHVKWAKTVRSSPDVIEMTFYPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKIPGLQRKEPVCPSLQF 360
           DGVAGKEHLTRAI LYLEYKPPLEELRYFLEFQIPRVWAP+ D IPG QRKEPVCPSLQF
Sbjct: 301 DGVAGKEHLTRAIRLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDAHVAI 420
           SIMGQKLYVSIEQI IGRKPVTGM LSLEG KQNRLSIHLQHLA+LPKI+ PYWD H+AI
Sbjct: 361 SIMGQKLYVSIEQILIGRKPVTGMCLSLEGGKQNRLSIHLQHLATLPKILQPYWDTHIAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVW FGS
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWGFGS 480

Query: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPQIERSKKLVSSSDNTN-NADNSSSDTKNTQGSN 540
           RNVLYMKLLYSRLPGCTIRRTLWDHLP  ++SKKLVSSSDN+N  AD+SSS +KN QG N
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPN-DKSKKLVSSSDNSNAAADDSSSGSKNIQG-N 540

Query: 541 KLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           KLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEK +IVLRVKYSLLNY
Sbjct: 541 KLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKSRIVLRVKYSLLNY 585

BLAST of Chy8G151830.1 vs. NCBI nr
Match: XP_011653720.1 (MACPF domain-containing protein CAD1 [Cucumis sativus] >KGN54446.1 hypothetical protein Csa_011952 [Cucumis sativus])

HSP 1 Score: 1164 bits (3012), Expect = 0.0
Identity = 571/586 (97.44%), Postives = 578/586 (98.63%), Query Frame = 0

Query: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVDVDDEHSRDICLYDDVVL 60
           MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIV+VDDEH+RDICLYDDVVL
Sbjct: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNCAFGFTGLKQTDA 120
           HNV WDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFN  FGFTGLKQTDA
Sbjct: 61  HNVPWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSVFGFTGLKQTDA 120

Query: 121 SATKSLSTDGFYIPLAKFQFTKTPLELRENVKRTVPTTWDPPSLASFIENFGTHVITSIT 180
           SATKSLSTDGFYIPLAKFQFTKTPLEL+ENVKR VPTTWDPPSLASFIENFGTHVITSIT
Sbjct: 121 SATKSLSTDGFYIPLAKFQFTKTPLELKENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQMGSGSIKLKEKGLEGGLFNN 240
           IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQ GSGSIKLKEKGLEGGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLEGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALL 300
           QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKIPGLQRKEPVCPSLQF 360
           DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKIPG QRKEPVCPSLQF
Sbjct: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKIPGRQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDAHVAI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIM PYWDAH+AI
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMVPYWDAHIAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVY+VTGAQLGVWDFGS
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYVVTGAQLGVWDFGS 480

Query: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPQIERSKKLVSSSDNTNNADNSSSDTKNTQGSNK 540
           RNVLYMKLLYSRLPGCTIRRTLWDHLP IE+SKKLVSSSDNTNNADNSSSDTKNTQG+NK
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSDTKNTQGNNK 540

Query: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 586
           LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 586

BLAST of Chy8G151830.1 vs. NCBI nr
Match: XP_008463260.1 (PREDICTED: MACPF domain-containing protein CAD1-like [Cucumis melo] >TYK09776.1 MACPF domain-containing protein CAD1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1160 bits (3000), Expect = 0.0
Identity = 568/586 (96.93%), Postives = 577/586 (98.46%), Query Frame = 0

Query: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVDVDDEHSRDICLYDDVVL 60
           MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIV+VDDEHSRDICLYDDVVL
Sbjct: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHSRDICLYDDVVL 60

Query: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNCAFGFTGLKQTDA 120
           HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFN AFGFTGL+QTDA
Sbjct: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSAFGFTGLRQTDA 120

Query: 121 SATKSLSTDGFYIPLAKFQFTKTPLELRENVKRTVPTTWDPPSLASFIENFGTHVITSIT 180
           SATKSLSTDGFYIPLAKFQFTKTPLEL+EN+KR VPTTWDPPSLASFIENFGTHVITSIT
Sbjct: 121 SATKSLSTDGFYIPLAKFQFTKTPLELKENIKRAVPTTWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQMGSGSIKLKEKGLEGGLFNN 240
           IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQ GSGSIKLKEKGL+GGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALL 300
           QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTF PITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKIPGLQRKEPVCPSLQF 360
           DGV GKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDK+PG QRKEPVCPSLQF
Sbjct: 301 DGVVGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKLPGRQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDAHVAI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWD HVAI
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480

Query: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPQIERSKKLVSSSDNTNNADNSSSDTKNTQGSNK 540
           RNVLYMKLLYSRLPGCTIRRTLWDHLP IE+SKKLVSSSDNTNNADNSSS TK+TQG+NK
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSGTKSTQGNNK 540

Query: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 586
           LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 586

BLAST of Chy8G151830.1 vs. NCBI nr
Match: XP_038882143.1 (MACPF domain-containing protein CAD1-like [Benincasa hispida])

HSP 1 Score: 1100 bits (2846), Expect = 0.0
Identity = 542/586 (92.49%), Postives = 562/586 (95.90%), Query Frame = 0

Query: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVDVDDEHSRDICLYDDVVL 60
           MGESGTALHTA NAVQALGRGFDVNFD+RLLYCKGV+GSTIV++DDEHSRDICLYDDVVL
Sbjct: 1   MGESGTALHTAMNAVQALGRGFDVNFDSRLLYCKGVSGSTIVEIDDEHSRDICLYDDVVL 60

Query: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNCAFGFTGLKQTDA 120
            NVSWDIKNFQEPIGHRSSGICSFDEM DYFNQKDGLSEGYPSGSFN AFGFTG KQ DA
Sbjct: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSAFGFTGFKQIDA 120

Query: 121 SATKSLSTDGFYIPLAKFQFTKTPLELRENVKRTVPTTWDPPSLASFIENFGTHVITSIT 180
           +ATKSLSTDGFYIPLAKFQFTKTPL L+ENVKR VPTTWDPPSLASFIENFGTHVITSIT
Sbjct: 121 AATKSLSTDGFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQMGSGSIKLKEKGLEGGLFNN 240
           IGGKDVIY+KQHQSSPLSTME+KRYVQDIANQRFSDTQGQMGSGSIK KEKGL+GGLFNN
Sbjct: 181 IGGKDVIYIKQHQSSPLSTMELKRYVQDIANQRFSDTQGQMGSGSIKHKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALL 300
           QGVYPQPTSAPYLTGKEDVTVIFRRRGG+DLEQNHVRWAR VRSSPDVIEMTF PITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGNDLEQNHVRWARNVRSSPDVIEMTFCPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKIPGLQRKEPVCPSLQF 360
           DGVAGKEHLTRAILLYL+YKPPLEELRYFLEFQIPRVWAP  D IPG QRKEPVCPSLQF
Sbjct: 301 DGVAGKEHLTRAILLYLDYKPPLEELRYFLEFQIPRVWAPTCDTIPGHQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDAHVAI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWD HVAI
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSH+STAPIENPETFMGELSGVYIVTGAQLGVWDFGS
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHISTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480

Query: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPQIERSKKLVSSSDNTNNADNSSSDTKNTQGSNK 540
           RNVLYMKLLYSRLPGCTIRR LWDHLP  ++SKKLV+SSDN++  D+SSS TK+ QG NK
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRMLWDHLPN-DKSKKLVTSSDNSSKVDDSSSGTKSIQG-NK 540

Query: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 586
           LAK+VDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKVVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 584

BLAST of Chy8G151830.1 vs. NCBI nr
Match: XP_022144069.1 (MACPF domain-containing protein CAD1-like [Momordica charantia])

HSP 1 Score: 1071 bits (2771), Expect = 0.0
Identity = 530/586 (90.44%), Postives = 553/586 (94.37%), Query Frame = 0

Query: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVDVDDEHSRDICLYDDVVL 60
           MG++GTALHTA NAVQALGRGFDVNFDTRLLYCKGV+GSTIV+VDDEH+RDICLYDDVVL
Sbjct: 1   MGDTGTALHTAINAVQALGRGFDVNFDTRLLYCKGVSGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNCAFGFTGLKQTDA 120
            NVSWDIKNFQEPIGHRSSGICSFDEM +YFNQKDGLSEGY SGSFN AFGF G KQ DA
Sbjct: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGFNGSKQIDA 120

Query: 121 SATKSLSTDGFYIPLAKFQFTKTPLELRENVKRTVPTTWDPPSLASFIENFGTHVITSIT 180
           + TK+LS DGFYIPLAKFQFTKTPL L+ENVKR +PT+WDPPSLASFIENFGTHVITSIT
Sbjct: 121 ATTKNLSMDGFYIPLAKFQFTKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQMGSGSIKLKEKGLEGGLFNN 240
           IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQG  GSGSIKLKEKGL+GGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGLTGSGSIKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALL 300
           QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVR SPDVIEMTF PITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRLSPDVIEMTFFPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKIPGLQRKEPVCPSLQF 360
           DGV GKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAP+ D +PG QRKEPVCPSLQF
Sbjct: 301 DGVTGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTVPGHQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDAHVAI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKI+ PYWD HV I
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHVGI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFG 
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGP 480

Query: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPQIERSKKLVSSSDNTNNADNSSSDTKNTQGSNK 540
           RNVLYMKLLYSRLPGCTI+RTLWDHL   ++SKKLVSSSDN+N AD+SSS ++N QG NK
Sbjct: 481 RNVLYMKLLYSRLPGCTIQRTLWDHLLN-DKSKKLVSSSDNSN-ADDSSSGSRNIQG-NK 540

Query: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 586
           LAKLVDT+EMSKGP D PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKLVDTSEMSKGPHDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 583

BLAST of Chy8G151830.1 vs. NCBI nr
Match: XP_022925277.1 (MACPF domain-containing protein CAD1-like [Cucurbita moschata])

HSP 1 Score: 1066 bits (2758), Expect = 0.0
Identity = 530/587 (90.29%), Postives = 552/587 (94.04%), Query Frame = 0

Query: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVDVDDEHSRDICLYDDVVL 60
           M ESGTALHTA NAVQALGRGFDVNFDTRLLYCKGVAGSTIV+VDDEH+RDICLYDDVVL
Sbjct: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNCAFGFTGLKQTDA 120
            NVSWDIKNFQ+PIGHRSSGICSFDEM DYFNQKDGLSEGYPSGSFN AFGFTG KQ DA
Sbjct: 61  LNVSWDIKNFQQPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNTAFGFTGSKQIDA 120

Query: 121 SATKSLSTDGFYIPLAKFQFTKTPLELRENVKRTVPTTWDPPSLASFIENFGTHVITSIT 180
           +A K+LSTDGFYIPLAKFQ T T L L ENVKR+VPT+WDPP+LASFIENFGTHVITSIT
Sbjct: 121 AAAKNLSTDGFYIPLAKFQLTSTSLLLHENVKRSVPTSWDPPALASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQMGSGSIKLKEKGLEGGLFNN 240
           IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQ GSGS KLKEKGL+GGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQAGSGSRKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALL 300
           QGVYPQPTSAPY +GKEDVTVIFRRRGGDDLEQNHV+WA+TVRSSPDVIEMTF PITALL
Sbjct: 241 QGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLEQNHVKWAKTVRSSPDVIEMTFYPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKIPGLQRKEPVCPSLQF 360
           DGVAGKEHLTRAI LYLEYKPPLEELRYFLEFQIPRVWAP+ D IPG QRKEPVCPSLQF
Sbjct: 301 DGVAGKEHLTRAIRLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDAHVAI 420
           SIMGQKLYVSIEQI IGRKPVTGM LSLEG KQNRLSIHLQHLA+LPKI+ PYWD H+AI
Sbjct: 361 SIMGQKLYVSIEQILIGRKPVTGMCLSLEGGKQNRLSIHLQHLATLPKILQPYWDTHIAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVW FGS
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWGFGS 480

Query: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPQIERSKKLVSSSDNTNNA-DNSSSDTKNTQGSN 540
           RNVLYMKLLYSRLPGCTIRRTLWDHLP  ++SKKLVSSSDN+N A D+SSS +KN QG N
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPN-DKSKKLVSSSDNSNAAADDSSSGSKNIQG-N 540

Query: 541 KLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 586
           KLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEK +IVLRVKYSLLNY
Sbjct: 541 KLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKSRIVLRVKYSLLNY 585

BLAST of Chy8G151830.1 vs. TAIR 10
Match: AT1G29690.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 694.5 bits (1791), Expect = 7.3e-200
Identity = 340/578 (58.82%), Postives = 426/578 (73.70%), Query Frame = 0

Query: 10  TASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVDVDDEHSRDICLYDDVVLHNVSWDIKN 69
           T  NA+QALGRGFDV  D RLLYCKG  GS +V +++  +RD+ L    +L NV  DI  
Sbjct: 20  TLRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPNVPADIDC 79

Query: 70  FQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNCAFGFTGLKQTDASATKSLSTD 129
            +   G +   +CSF EMA+ FN + G+    P G FN  F +TG  Q DA++TKSL+  
Sbjct: 80  SRGNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALV 139

Query: 130 GFYIPLAKFQFTKTPLELRENVKRTVPTTWDPPSLASFIENFGTHVITSITIGGKDVIYV 189
           G++IPL   +  K  L L   ++R VP++WDP SLASFIEN+GTH++TS+TIGG+DV+Y+
Sbjct: 140 GYFIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYI 199

Query: 190 KQHQSSPLSTMEVKRYVQDIANQRFSDTQGQMGSGSIKLKEKGLEGGLFNNQGVYPQPTS 249
           +QHQSSPL   E++ YV D+   RF + + Q  +G +K K+K                  
Sbjct: 200 RQHQSSPLPVSEIENYVNDMIKHRFHEAESQSITGPLKYKDK------------------ 259

Query: 250 APYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALLDGVAGKEHL 309
                   D+TVIFRRRGGDDLEQ+H RWA TV ++PD+I MTF PI +LL+GV G  HL
Sbjct: 260 --------DITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHL 319

Query: 310 TRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKIPGLQRKEPVCPSLQFSIMGQKLYV 369
           TRAI LYLEYKPP+E+L+YFL++QI R WAP   +   LQRKEPVC SLQFS+MG KL++
Sbjct: 320 TRAIELYLEYKPPIEDLQYFLDYQIARAWAP---EQSNLQRKEPVCSSLQFSLMGPKLFI 379

Query: 370 SIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDAHVAIGAPKWQGPE 429
           S +Q+++GRKPVTG+RLSLEGSKQNRLSIHLQHL SLPKI+ P+WD+HV IGAPKWQGPE
Sbjct: 380 SADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWDSHVPIGAPKWQGPE 439

Query: 430 EQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGSRNVLYMKLL 489
           EQDSRWFEP+KWKNFSHVST+PIE+ ET +G+LSGV+IVTGAQLGVWDFGS+NVL++KLL
Sbjct: 440 EQDSRWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLKLL 499

Query: 490 YSRLPGCTIRRTLWDHLPQIERSKKLVSSSDNTNNADNSSSDTKNTQG-SNKLAKLVDTT 549
           +S++PGCTIRR++WDH P       + SS        ++SS T+   G S KLAK+VD++
Sbjct: 500 FSKVPGCTIRRSVWDHTP-------VASSGRLEPGGPSTSSSTEEVSGQSGKLAKIVDSS 559

Query: 550 EMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           EM KGPQD PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 560 EMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 561

BLAST of Chy8G151830.1 vs. TAIR 10
Match: AT4G24290.2 (MAC/Perforin domain-containing protein )

HSP 1 Score: 426.8 bits (1096), Expect = 2.8e-119
Identity = 254/607 (41.85%), Postives = 363/607 (59.80%), Query Frame = 0

Query: 7   ALHTASNAVQALGRGFDVNFDTRLLYCKGVA-GSTIVDV-DDEHSRDICLYDDVVLHNVS 66
           A   A  A+ ++G G+D+  D RL YCKG +  S ++D+ + + + +I L   + + NVS
Sbjct: 7   ASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVS 66

Query: 67  WDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNCAFGFTGLKQTDASATK 126
             IK  +       S I  F +MA+ FNQ+  L+   PSG FN  F F+   Q DA+ TK
Sbjct: 67  KSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTK 126

Query: 127 SLSTDGFYIPLAKFQFTKTPLELRENVKRTVPTTWDPPSLASFIENFGTHVITSITIGGK 186
           +L+ DG +I L      K+ + LRE+VK+ VP+TWDP +LA FI+ +GTH+I S+ +GGK
Sbjct: 127 NLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGK 186

Query: 187 DVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQMGSGS--------IKLKEKGLEGG 246
           DVIY KQ  SS L   ++++ ++++A++RF +      +GS        ++ KE+ L   
Sbjct: 187 DVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFA 246

Query: 247 LFNNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQ-NHVRWARTVRSSPDVIEMTFNP 306
             ++ G Y           KED   + +RRGG+D     H  W +TV+  PDVI M+F P
Sbjct: 247 DTSSLGSY---------ANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIP 306

Query: 307 ITALLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKIP-GLQRKEPV 366
           IT+LL+GV G   L+ AI LYL YKPP+EEL  FLEFQ+PR WAP+  ++P G QRK+  
Sbjct: 307 ITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQS 366

Query: 367 CPSLQFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYW 426
           C SLQFS  G KLYV+   + +G++P+TGMRL LEG + NRL+IHLQHL+SLPKI     
Sbjct: 367 CASLQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLED 426

Query: 427 DAHVAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLG 486
           D + +I        E  D R++E V WKN+SHV T P+E+ +    +LS   +VTGAQL 
Sbjct: 427 DLNRSI------RQESHDRRYYEKVNWKNYSHVCTEPVESDD----DLS---VVTGAQLH 486

Query: 487 VWDFGSRNVLYMKLLYSRLPGCT-IRRTLWDHLPQIERSKKLVS---------------- 546
           V   G +NVL+++L +SR+ G T ++ + WD          L+S                
Sbjct: 487 VESHGFKNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPR 546

Query: 547 -SSDNTNNADNSSSDTKNTQGSNKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIV 584
            +  N N+A         TQ + KL K VDT+EM++GPQ+SPG+W+V+GA+L VEKGKI 
Sbjct: 547 PADVNINSAIYPGGPPVPTQ-APKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKIS 590

BLAST of Chy8G151830.1 vs. TAIR 10
Match: AT1G28380.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 390.2 bits (1001), Expect = 2.9e-108
Identity = 238/602 (39.53%), Postives = 345/602 (57.31%), Query Frame = 0

Query: 11  ASNAVQALGRGFDVNFDTRLLYCKGVA-GSTIVDVDDEHSRDICLYDDVVLHNVSWDIKN 70
           A  AV  +G G+D+  D R   CK    GS +V++D   +RD+     +V++NVS  IK 
Sbjct: 15  AEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIKC 74

Query: 71  FQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNCAFGFTGLKQTDASATKSLSTD 130
            +       S I SF++M++ FNQ   LS   PSG FN  F F+     DAS+ K+L+ D
Sbjct: 75  DKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLAYD 134

Query: 131 GFYIPLAKFQFTKTPLELRENVKRTVPTTWDPPSLASFIENFGTHVITSITIGGKDVIYV 190
           G++I L   +  +  L LR+ VKR VP++WD  +LA FIE +GTHV+  +T+GGKDVI+V
Sbjct: 135 GWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIHV 194

Query: 191 KQHQSSPLSTMEVKRYVQDIANQRF------SDTQGQMGSGSIKLKEKGLEGGLFNNQGV 250
           KQ + S     E+++ ++   ++RF      S +   + SG  K +E  L+ GL   Q  
Sbjct: 195 KQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPK-EENLLQWGL---QPF 254

Query: 251 YPQPTSAPYL-TGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALLDG 310
               +SA  + T  E++  +  RRGG DL Q+H RW  TV  +P+VI M F PIT+LL G
Sbjct: 255 GTSVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSLLSG 314

Query: 311 VAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKIP-GLQRKEPVCPSLQFS 370
           + G   L+ A+ LYL YKPP+EEL  FLEFQ+PR WAP++  +P GL+R +   PSLQFS
Sbjct: 315 LPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQFS 374

Query: 371 IMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMH-PYWDAHVAI 430
           +MG KLYV+  ++  G +PVTG+R  LEG K N L+IHLQHL++ P  +H  + D +  I
Sbjct: 375 LMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEPI 434

Query: 431 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 490
             P  +G       ++ PVKW  FSHV T P++       + +   IVT A L V   G 
Sbjct: 435 EEPVEKG-------YYVPVKWGIFSHVCTYPVQYNGARSDDTAS--IVTKAWLEVKGMGM 494

Query: 491 RNVLYMKLLYSRLPGCTIRRTLWDHLPQIERSK---KLVSSSDNTNNADNSSSDTKNTQ- 550
           R VL+++L +S       R++ WD+L    R      ++S+  +T  + N ++    ++ 
Sbjct: 495 RKVLFLRLGFSLDASAVTRKSCWDNLSTNSRKSGVFSMISTRLSTGLSPNPATTKPQSKI 554

Query: 551 --------------GSNKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYS 585
                            KL  LVDT E+ +GP++ PG+W+VTGAKL VE GKI ++ KYS
Sbjct: 555 DINSAVYPRGPSPPVKPKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYS 603

BLAST of Chy8G151830.1 vs. TAIR 10
Match: AT1G14780.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 359.8 bits (922), Expect = 4.3e-99
Identity = 223/623 (35.79%), Postives = 333/623 (53.45%), Query Frame = 0

Query: 14  AVQALGRGFDVNFDTRLLYCKGVAGST----IVDVDDEHSRDICLYDDVVLHNVSWDIKN 73
           AV++LG+GFD+  D RL YCK   GS     +V +D   +R++ +    V  NVS DI  
Sbjct: 12  AVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADINC 71

Query: 74  FQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNCAFGF-TGLKQTDASATKSLST 133
            +       S I  F++M++YFNQ+  ++   PSG+FN  FGF +G   TDA+  KSL  
Sbjct: 72  DKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSLGL 131

Query: 134 DGFYIPLAKFQF-TKTPLELRENVKRTVPTTWDPPSLASFIENFGTHVITSITIGGKDVI 193
           D   + L          L L + V+  VP++WDP  LA FIE +GTHVIT +++GG+DV+
Sbjct: 132 DASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQDVV 191

Query: 194 YVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQMGSGSIKLKEKGLEGGLFNNQ------ 253
            V+Q +SS L    ++ ++ D+ +Q F        +GS  L  + L     ++       
Sbjct: 192 VVRQDKSSDLDNDLLRHHLYDLGDQLF--------TGSCLLSTRRLNKAYHHSHSQPKFP 251

Query: 254 ---GVYPQPTSAPY----LTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFN 313
               V+    +  +    +  +  +TVI  +RGGD   ++H  W  TV   PD I   F 
Sbjct: 252 EAFNVFDDKQTVAFNNFSINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFI 311

Query: 314 PITALLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKIP--GLQRKE 373
           PIT+LL  V G   L+ A+ LYL YKPPL +L+YFL+F  PR WAP+H+ +P        
Sbjct: 312 PITSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMA 371

Query: 374 PVCPSLQFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASL-PKIMH 433
              P+L  + MG KLYV+   ++  + PVTGMR  LEG K NRL+IHLQHL +    +  
Sbjct: 372 SAYPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGE 431

Query: 434 PYWDAHVAIGAPKWQGPEE--QDSRWFEPVKWKNFSHVSTAPIENPETFMGELSG----- 493
              D H+      W+G ++   + R+FEP+  K FSHV T P++    ++   S      
Sbjct: 432 KITDEHI------WRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQN 491

Query: 494 --VYIVTGAQLGVWDFGSRNVLYMKLLYSRLPGCTIRRTLWDHLPQIERSKK-------- 553
              +IVTGAQL V   GS++VL+++L Y+++    + +  W H P I  S+K        
Sbjct: 492 DVAFIVTGAQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGP-IGTSQKSGIFSSMS 551

Query: 554 --LVSSSDNTNNADNSSSDTKNTQG----------SNKLAKLVDTTEMSKGPQDSPGHWL 586
             L S S + N      ++     G          +NK+ K VD +++ +GPQ SPGHWL
Sbjct: 552 MPLTSGSVHHNMIQKDKNEVVLDSGVFPGGPPVPANNKIVKFVDLSQLCRGPQHSPGHWL 611

BLAST of Chy8G151830.1 vs. TAIR 10
Match: AT4G24290.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 207.6 bits (527), Expect = 2.7e-53
Identity = 129/338 (38.17%), Postives = 193/338 (57.10%), Query Frame = 0

Query: 7   ALHTASNAVQALGRGFDVNFDTRLLYCKGVA-GSTIVDV-DDEHSRDICLYDDVVLHNVS 66
           A   A  A+ ++G G+D+  D RL YCKG +  S ++D+ + + + +I L   + + NVS
Sbjct: 7   ASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVS 66

Query: 67  WDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNCAFGFTGLKQTDASATK 126
             IK  +       S I  F +MA+ FNQ+  L+   PSG FN  F F+   Q DA+ TK
Sbjct: 67  KSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTK 126

Query: 127 SLSTDGFYIPLAKFQFTKTPLELRENVKRTVPTTWDPPSLASFIENFGTHVITSITIGGK 186
           +L+ DG +I L      K+ + LRE+VK+ VP+TWDP +LA FI+ +GTH+I S+ +GGK
Sbjct: 127 NLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGK 186

Query: 187 DVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQMGSGS--------IKLKEKGLEGG 246
           DVIY KQ  SS L   ++++ ++++A++RF +      +GS        ++ KE+ L   
Sbjct: 187 DVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFA 246

Query: 247 LFNNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQ-NHVRWARTVRSSPDVIEMTFNP 306
             ++ G Y           KED   + +RRGG+D     H  W +TV+  PDVI M+F P
Sbjct: 247 DTSSLGSY---------ANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIP 306

Query: 307 ITALLDGVAGKEHLTRAILLYLEYKP--PLEELRYFLE 332
           IT+LL+GV G   L+ AI LYL  KP   L  + +FLE
Sbjct: 307 ITSLLNGVPGSGFLSHAINLYLRCKPINHLRFVHFFLE 335