Chy4G075310.1 (mRNA) Cucumber (hystrix) v1

Overview


Name	Chy4G075310.1
Type	mRNA
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Glucan endo-1,3-beta-D-glucosidase
Location	chrH04: 10601417 .. 10603765 (+)
Sequence length	1398
RNA-Seq Expression	Chy4G075310.1
Synteny	Chy4G075310.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCCATTCCCTTCCTTTCTCTACTCTTCTTTCTCCTTTTCCTCTCACTCTCAAGTAAACTCCAATATCCTTCTTCTTTTTCTTCTTCTTCTTCTTCTACATTTTTCCATTTCTCTCTAACCTTCTCCTCCTTCATTTCTTGCAGGTGCAGAAATCTCCACCCTCGTCGGAATCAACTACGGTCAGCTCGGCAACAACCTCCCTTCGCCCTCCCATTCCGTCAATTTAATCAAATCCCTTAATGCCCAAATCGTCAAAATCTACGACGCAAATCCCCAAATCCTCAAAGCTCTCAAAAACACCGACCTACGGGTCTCCGTCATGGTTCCTAACGAACTCATCATCAACATTTCTTCTAGCCAGAACCTCGCCGACCAATGGGTTCGGACCAATATCCTCCCTTTTTACCCCCAAACCAAAATCCGTTACCTTCTCGTCGGAAATGAAATCATCTCCTCCACTGGAAATCAAACCTGGTTCAGTTTAGTACCGGCGATGCGCCGAATCAAACATTCTTTGAAAACTTACGGGATTCATAAAGTGAAAGTCGGAACACCCTCTGCTATGGATGTTCTTCAGTCATCTTTTCCGCCTTCGAATGGGACATTCCGGGCGGATATTGCCGATCGTGTCATGAGACCGATGTTGCAGTTTCTTAACCGGACGAAATCGTTCTTCTTCCTTGATGTGTATCCCTATTTCCCTTGGTCTTCCGATTCAGTTAACATTAAACTCGATTATGCTCTGTTTGAATCGAAGAACATTACGTATTCGGATCCGGTTTCGGGCTTGGTTTACACGAATCTCTTCGATCAAATGGTGGATTCAGTGATTTTCGCTATGAAACATCTCGGCTTCCCCGAAATCCGAGTATTCATCGCCGAAACCGGATGGCCTAACGGCGGCGATTACGACCAAATCGGCGCCAACATCCATAATGCGGCTGTGTACAACCGGAACATCGTGAAACGAGTCACTTCGAAGCCGCCGGTTGGTACACCGGCTCGACCGGGACGGGTGTTGCCGACGATAATATTCTCTCTGTATAATGAGAATGAGAAACCGGGTCCGGGTACGGAGAGGCATTTCGGACTGTTGTATCCGAACGGAAAGGCGGTTTATGAGATGGATTTGAGTGGGTCGACAGCGGAGACAAAGTTTAAGCCGCTGCCAGAGGCGGAGAGGAATGAACCGTACAAGGGGAAGATTTGGTGCGTAGTTGGGAAGGGGGTGAATATGAGTGATGTTGTTGGGGCTTTATCGTACGCTTGCTCCCAGGGAAACAAAACGTGCGAACCGATAGGGAAGGGGGGTCCGTGTTATGAACCGGATTCTTTAAAACGACATGCCAGTTTTGCGTTTAGTTCGTACTGGGCCCAGTTTAGAAAGGTCGGTGGAACCTGTTACTTTAATGGCTTAGCAACTCAAACCATCAAAGACCCAAGTAAGCTTTTGTTATTATTATTATTATTTTTTTAATATTTAATTTTTTCAATTTTAACTCGGACCAAAGAAAATAATAATTATTATCGCTTAATTGAAAGACCTAAGTATGTTAATATTATTATTATCACCATTTTTAAACAAATTGAATTCCAACCTGAACAAATAGTACATCTTAATTAGCTTAGAGTGATTTTAAAATATTCAATTTCAAAATCATTTTTAATATTTTCAAAATCAGTATAAAACTTAGTTTTAATCATTACAATAGCTTCTAAACATTTATTTCTTTTTAATCTTTTTAATTCTATCATGAATGTTTTTTCAAGGTATGAAAGTTTGGTGTGCTATTAATTGTTAGAACATATTTAGATATGGTTGCACACCAGTTGGACATAATAGAAGTAATGGTTTTTTTTCCTTCTTAAGTTGCATTCATTGTTATATTCACTTGTTCAAACAAAACAATAATTTAATGACTTGAATGGTTGTTAACAACTATATGTAAAATCCACTTTTTTAGTAAAATAGACATTGGATAGTGAAAATCAAACATTTGAACTCAAAAAAAAAAGAACACATATCAATTATTCTTTACAAGATTTAGTAACACGTTTTTATTTTCTATGAATTTCAAATAATTGAGGTTGTTTGAAATTTATTGTTTTTATAATAATAATAATATAGTATATAAGTAGATTGAGAATGATAATATAGATATGAGGTAATTTTATTAGCATATTATATAGTAAGAAAGATGTTGAGATTATGTTTGAGAGAGACGATAGGTGAGTAGAACTTAATTAGTTAATTTACTTCCAAATATTGGTGAACTAAAGAAAAATAATTAATATTTTGCAGGTTATGGAAAATGCAAGTTCCCAAGTGTGACATTGCAAGCATAA

mRNA sequence

ATGCCCATTCCCTTCCTTTCTCTACTCTTCTTTCTCCTTTTCCTCTCACTCTCAAGTGCAGAAATCTCCACCCTCGTCGGAATCAACTACGGTCAGCTCGGCAACAACCTCCCTTCGCCCTCCCATTCCGTCAATTTAATCAAATCCCTTAATGCCCAAATCGTCAAAATCTACGACGCAAATCCCCAAATCCTCAAAGCTCTCAAAAACACCGACCTACGGGTCTCCGTCATGGTTCCTAACGAACTCATCATCAACATTTCTTCTAGCCAGAACCTCGCCGACCAATGGGTTCGGACCAATATCCTCCCTTTTTACCCCCAAACCAAAATCCGTTACCTTCTCGTCGGAAATGAAATCATCTCCTCCACTGGAAATCAAACCTGGTTCAGTTTAGTACCGGCGATGCGCCGAATCAAACATTCTTTGAAAACTTACGGGATTCATAAAGTGAAAGTCGGAACACCCTCTGCTATGGATGTTCTTCAGTCATCTTTTCCGCCTTCGAATGGGACATTCCGGGCGGATATTGCCGATCGTGTCATGAGACCGATGTTGCAGTTTCTTAACCGGACGAAATCGTTCTTCTTCCTTGATGTGTATCCCTATTTCCCTTGGTCTTCCGATTCAGTTAACATTAAACTCGATTATGCTCTGTTTGAATCGAAGAACATTACGTATTCGGATCCGGTTTCGGGCTTGGTTTACACGAATCTCTTCGATCAAATGGTGGATTCAGTGATTTTCGCTATGAAACATCTCGGCTTCCCCGAAATCCGAGTATTCATCGCCGAAACCGGATGGCCTAACGGCGGCGATTACGACCAAATCGGCGCCAACATCCATAATGCGGCTGTGTACAACCGGAACATCGTGAAACGAGTCACTTCGAAGCCGCCGGTTGGTACACCGGCTCGACCGGGACGGGTGTTGCCGACGATAATATTCTCTCTGTATAATGAGAATGAGAAACCGGGTCCGGGTACGGAGAGGCATTTCGGACTGTTGTATCCGAACGGAAAGGCGGTTTATGAGATGGATTTGAGTGGGTCGACAGCGGAGACAAAGTTTAAGCCGCTGCCAGAGGCGGAGAGGAATGAACCGTACAAGGGGAAGATTTGGTGCGTAGTTGGGAAGGGGGTGAATATGAGTGATGTTGTTGGGGCTTTATCGTACGCTTGCTCCCAGGGAAACAAAACGTGCGAACCGATAGGGAAGGGGGGTCCGTGTTATGAACCGGATTCTTTAAAACGACATGCCAGTTTTGCGTTTAGTTCGTACTGGGCCCAGTTTAGAAAGGTCGGTGGAACCTGTTACTTTAATGGCTTAGCAACTCAAACCATCAAAGACCCAAGTTATGGAAAATGCAAGTTCCCAAGTGTGACATTGCAAGCATAA

Coding sequence (CDS)

ATGCCCATTCCCTTCCTTTCTCTACTCTTCTTTCTCCTTTTCCTCTCACTCTCAAGTGCAGAAATCTCCACCCTCGTCGGAATCAACTACGGTCAGCTCGGCAACAACCTCCCTTCGCCCTCCCATTCCGTCAATTTAATCAAATCCCTTAATGCCCAAATCGTCAAAATCTACGACGCAAATCCCCAAATCCTCAAAGCTCTCAAAAACACCGACCTACGGGTCTCCGTCATGGTTCCTAACGAACTCATCATCAACATTTCTTCTAGCCAGAACCTCGCCGACCAATGGGTTCGGACCAATATCCTCCCTTTTTACCCCCAAACCAAAATCCGTTACCTTCTCGTCGGAAATGAAATCATCTCCTCCACTGGAAATCAAACCTGGTTCAGTTTAGTACCGGCGATGCGCCGAATCAAACATTCTTTGAAAACTTACGGGATTCATAAAGTGAAAGTCGGAACACCCTCTGCTATGGATGTTCTTCAGTCATCTTTTCCGCCTTCGAATGGGACATTCCGGGCGGATATTGCCGATCGTGTCATGAGACCGATGTTGCAGTTTCTTAACCGGACGAAATCGTTCTTCTTCCTTGATGTGTATCCCTATTTCCCTTGGTCTTCCGATTCAGTTAACATTAAACTCGATTATGCTCTGTTTGAATCGAAGAACATTACGTATTCGGATCCGGTTTCGGGCTTGGTTTACACGAATCTCTTCGATCAAATGGTGGATTCAGTGATTTTCGCTATGAAACATCTCGGCTTCCCCGAAATCCGAGTATTCATCGCCGAAACCGGATGGCCTAACGGCGGCGATTACGACCAAATCGGCGCCAACATCCATAATGCGGCTGTGTACAACCGGAACATCGTGAAACGAGTCACTTCGAAGCCGCCGGTTGGTACACCGGCTCGACCGGGACGGGTGTTGCCGACGATAATATTCTCTCTGTATAATGAGAATGAGAAACCGGGTCCGGGTACGGAGAGGCATTTCGGACTGTTGTATCCGAACGGAAAGGCGGTTTATGAGATGGATTTGAGTGGGTCGACAGCGGAGACAAAGTTTAAGCCGCTGCCAGAGGCGGAGAGGAATGAACCGTACAAGGGGAAGATTTGGTGCGTAGTTGGGAAGGGGGTGAATATGAGTGATGTTGTTGGGGCTTTATCGTACGCTTGCTCCCAGGGAAACAAAACGTGCGAACCGATAGGGAAGGGGGGTCCGTGTTATGAACCGGATTCTTTAAAACGACATGCCAGTTTTGCGTTTAGTTCGTACTGGGCCCAGTTTAGAAAGGTCGGTGGAACCTGTTACTTTAATGGCTTAGCAACTCAAACCATCAAAGACCCAAGTTATGGAAAATGCAAGTTCCCAAGTGTGACATTGCAAGCATAA

Protein sequence

MPIPFLSLLFFLLFLSLSSAEISTLVGINYGQLGNNLPSPSHSVNLIKSLNAQIVKIYDANPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEIISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADRVMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLFDQMVDSVIFAMKHLGFPEIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPPVGTPARPGRVLPTIIFSLYNENEKPGPGTERHFGLLYPNGKAVYEMDLSGSTAETKFKPLPEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIGKGGPCYEPDSLKRHASFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQA*

Homology

[Close]

BLAST of Chy4G075310.1 vs. ExPASy Swiss-Prot
Match: Q06915 (Probable glucan endo-1,3-beta-glucosidase A6 OS=Arabidopsis thaliana OX=3702 GN=A6 PE=2 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 4.4e-152
Identity = 246/443 (55.53%), Postives = 333/443 (75.17%), Query Frame = 0


Query: 21  EISTLVGINYGQLGNNLPSPSHSVNLIKSLNAQIVKIYDANPQILKALKNTDLRVSVMVP 80
           ++++ +GINYG+ GNNLPSP  S+N IKS+ A  VK+YDA+P+ L  L  T+L V++ VP
Sbjct: 37  DLASKIGINYGRRGNNLPSPYQSINFIKSIKAGHVKLYDADPESLTLLSQTNLYVTITVP 96

Query: 81  NELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEIISSTGNQTWFSLVPAMRRIK 140
           N  I  +SS+Q +AD+WVRTNILP+YPQT+IR++LVGNEI+S        +LVPAMR+I 
Sbjct: 97  NHQITALSSNQTIADEWVRTNILPYYPQTQIRFVLVGNEILSYNSGNVSVNLVPAMRKIV 156

Query: 141 HSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADRVMRPMLQFLNRTKSFFFLDV 200
           +SL+ +GIH +KVGTP AMD L+SSFPPSNGTFR +I   VM P+L+FLN T S+FFL+V
Sbjct: 157 NSLRLHGIHNIKVGTPLAMDSLRSSFPPSNGTFREEITGPVMLPLLKFLNGTNSYFFLNV 216

Query: 201 YPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLFDQMVDSVIFAMKHLGFPEIR 260
           +PYF WS + +N  LD+ALF+  + TY+DP +GLVY NL DQM+DSV+FAM  LG+P +R
Sbjct: 217 HPYFRWSRNPMNTSLDFALFQGHS-TYTDPQTGLVYRNLLDQMLDSVLFAMTKLGYPHMR 276

Query: 261 VFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPPVGTPARPGRVLPTIIFSLYN 320
           + I+ETGWPN GD D+ GANI NAA YNRN++K++++ PP+GTP+RPG  +PT +FSL+N
Sbjct: 277 LAISETGWPNFGDIDETGANILNAATYNRNLIKKMSASPPIGTPSRPGLPIPTFVFSLFN 336

Query: 321 ENEKPGPGTERHFGLLYPNGKAVYEMDLSGSTAETKFKPLPEAERNEPYKGKIWCVVGKG 380
           EN+K G GT+RH+G+L+P+G  +Y++D +G T  T F PLP+   N PYKG++WCV  +G
Sbjct: 337 ENQKSGSGTQRHWGILHPDGSPIYDVDFTGQTPLTGFNPLPKPTNNVPYKGQVWCVPVEG 396

Query: 381 VNMSDVVGALSYACSQGNKTCEPIGKGGPCYEPDSLKRHASFAFSSYWAQFRKVGGTCYF 440
            N +++   L  AC+Q N TC  +  G  CYEP S+  HAS+A +SYWAQFR     C+F
Sbjct: 397 ANETELEETLRMACAQSNTTCAALAPGRECYEPVSIYWHASYALNSYWAQFRNQSIQCFF 456

Query: 441 NGLATQTIKDPSYGKCKFPSVTL 464
           NGLA +T  +P   +CKFPSVTL
Sbjct: 457 NGLAHETTTNPGNDRCKFPSVTL 478

[Close]

BLAST of Chy4G075310.1 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 1.6e-90
Identity = 171/433 (39.49%), Postives = 250/433 (57.74%), Query Frame = 0


Query: 26  VGINYGQLGNNLPSPSHSVNLIKSLNAQIVKIYDANPQILKALKNTDLRVSVMVPNELII 85
           VG+ YG+  ++LP+PS  V LI+  N + V+IYD N Q+LKA  NT + + + VPN  + 
Sbjct: 26  VGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYDYNSQVLKAFGNTSIELMIGVPNSDLN 85

Query: 86  NISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEIISSTGNQTWFSLVPAMRRIKHSLKT 145
             S SQ+  D W++ ++LP+YP TKI Y+ VG E            +VPAM+ +  +L+ 
Sbjct: 86  AFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAESTDDPHINASSFVVPAMQNVLTALRK 145

Query: 146 YGI-HKVKVGTPSAMDVLQSSFPPSNGTFRADIADRVMRPMLQFLNRTKSFFFLDVYPYF 205
            G+  ++KV T  ++ +L  SFPPS G F +  A   +RPML+FL   KS F +D+YPY+
Sbjct: 146 VGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSYA-YFLRPMLEFLAENKSPFMIDLYPYY 205

Query: 206 PWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLFDQMVDSVIFAMKHLGFPEIRVFIA 265
            +     N+ LDY LFES +    DP +GL+Y N+FD  VD++ +A+  L F  I++ + 
Sbjct: 206 AYRDSPNNVSLDYVLFESSS-EVIDPNTGLLYKNMFDAQVDALYYALTALNFRTIKIMVT 265

Query: 266 ETGWP-NGGDYDQIGANIHNAAVYNRNIVKRVTSKPPVGTPARPGRVLPTIIFSLYNENE 325
           ETGWP  G   ++  A+  NA  YN NI++ V +    GTPA+PG  +   IFSL+NEN 
Sbjct: 266 ETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQ--GTPAKPGEAMNVYIFSLFNENR 325

Query: 326 KPGPGTERHFGLLYPNGKAVYEMDLSGSTAETKFKPLPEAERNEPYKGKIWCVVGKGVNM 385
           K G  +ER++GL YP+  +VY++D +G +          +  N       WC+     + 
Sbjct: 326 KAGLDSERNWGLFYPDQTSVYQLDFTGKSNGFHSN---SSGTNSSGSSNSWCIASSKASE 385

Query: 386 SDVVGALSYACSQGNKTCEPIGKGGPCYEPDSLKRHASFAFSSYWAQFRKVGGTCYFNGL 445
            D+ GAL +AC  GN  C  I    PC++PD+L  HASF F+SY+ Q R     C F G 
Sbjct: 386 RDLKGALDWACGPGNVDCTAIQPSQPCFQPDTLVSHASFVFNSYFQQNRATDVACSFGGA 445

Query: 446 ATQTIKDPSYGKC 457
             +  KDPSY KC
Sbjct: 446 GVKVNKDPSYDKC 451

[Close]

BLAST of Chy4G075310.1 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 1.5e-88
Identity = 172/476 (36.13%), Postives = 268/476 (56.30%), Query Frame = 0


Query: 5   FLSLLFFLLFLSLSSAEISTLVGINYGQLGNNLPSPSHSVNLIKSLNAQIVKIYDANPQI 64
           F+S+L FL F       +++ +GI YG+  +NLPSP+    LI+ LN + V+IYDAN  +
Sbjct: 12  FVSILAFLNF------GMASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDV 71

Query: 65  LKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEIISST 124
           LKA  NT + + + VPN  ++  +  Q+  D W+  NILP+YP TKI  + VG E+  + 
Sbjct: 72  LKAFANTGIELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAP 131

Query: 125 GNQTWFSLVPAMRRIKHSLKTYGI-HKVKVGTPSAMDVLQSSFPPSNGTFRADIADRVMR 184
            N T   ++PAMR I  +LK  G+  K+K+ +  ++ +L  SFPPS+ +F +      ++
Sbjct: 132 DNATGL-VLPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASF-SKKHSAFLK 191

Query: 185 PMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLFDQM 244
           PML+FL   +S F +D+YPY+ +   +  + L+YALFES +    DP +GL+Y+N+FD  
Sbjct: 192 PMLEFLVENESPFMIDLYPYYAYRDSTEKVPLEYALFESSS-QVVDPATGLLYSNMFDAQ 251

Query: 245 VDSVIFAMKHLGFPEIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPPVGT 304
           +D++ FA+  + F  ++V + E+GWP+ G   +  A   NA  YN N+++ V   P  GT
Sbjct: 252 LDAIYFALTAMSFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDP--GT 311

Query: 305 PARPGRVLPTIIFSLYNENEKPGPGTERHFGLLYPNGKAVYEMDLSGSTAETKFKPLPEA 364
           PA+PG  +   +FSL+NEN KPG  +ER++G+ Y NG  VY +D +G    T   P    
Sbjct: 312 PAKPGEEIDVYLFSLFNENRKPGIESERNWGMFYANGTNVYALDFTGENT-TPVSPTNST 371

Query: 365 ERNEPYKG---------------------KIWCVVGKGVNMSDVVGALSYACSQGNKTCE 424
               P                        K WC+     +++++  AL +AC  GN  C 
Sbjct: 372 TGTSPSPSSSPIINGNSTVTIGGGGGGGTKKWCIASSQASVTELQTALDWACGPGNVDCS 431

Query: 425 PIGKGGPCYEPDSLKRHASFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKF 459
            +    PC+EPD++  HAS+AF++Y+ Q       C FNG + +  KDPSYG C +
Sbjct: 432 AVQPDQPCFEPDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEVDKDPSYGNCLY 475

[Close]

BLAST of Chy4G075310.1 vs. ExPASy Swiss-Prot
Match: Q94G86 (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 7.6e-88
Identity = 172/456 (37.72%), Postives = 266/456 (58.33%), Query Frame = 0


Query: 6   LSLLFFLLFLSLSSAEISTLVGINYGQLGNNLPSPSHSVNLIKSLNAQIVKIYDANPQIL 65
           L  L FLL  +  SA   + +G+NYGQL +NLPS   +VNL+KS   Q V+++ A P ++
Sbjct: 10  LLFLVFLLLQNFYSANSQSFLGVNYGQLSDNLPSLQATVNLLKSTTIQKVRLFGAEPAVI 69

Query: 66  KALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEIISSTG 125
           KA  NT + + +   N  I  ++S+ N+A Q+V++N++ FYP + I  + VGNE+++S  
Sbjct: 70  KAFANTGVEIVIGFDNGDIPTLASNPNVASQFVKSNVMSFYPASNIIAITVGNEVLTSGD 129

Query: 126 NQTWFSLVPAMRRIKHSLKTYGI-HKVKVGTPSAMDVLQSSFPPSNGTFRADIADRVMRP 185
            +    L+PAM+ ++++L    +  KVKV T  AM VL  S+PPS+G F   + D  M+ 
Sbjct: 130 QKLISQLLPAMQNVQNALNAASLGGKVKVSTVHAMAVLSQSYPPSSGVFNPGLGD-TMKA 189

Query: 186 MLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLFDQMV 245
           +LQF +   + F +  YPYF + +      L + LF+  N    D  +G  YTN+FD  V
Sbjct: 190 LLQFQSANDAPFMISPYPYFAYKNQPTPDTLAFCLFQ-PNAGQVDSGNGHKYTNMFDAQV 249

Query: 246 DSVIFAMKHLGFPEIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPPVGTP 305
           D+V  A+  +GF +I + +AETGWP+GGD +++G ++ NA  Y  N++  + SK  VGTP
Sbjct: 250 DAVHSALNAMGFKDIEIVVAETGWPHGGDSNEVGPSLDNAKAYVGNLINHLKSK--VGTP 309

Query: 306 ARPGRVLPTIIFSLYNENEKPGPGTERHFGLLYPNGKAVYEMDLSGSTAETKFKPLPEAE 365
             PG+ + T +FSLY+E++K G  +E++FGL  P+G   Y++   G    T+    P   
Sbjct: 310 LMPGKSIDTYLFSLYDEDKKTGASSEKYFGLFKPDGSTTYDV---GLLKNTQNPTTPATP 369

Query: 366 RNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIGKGGPCYEPDSLKRHASFAF 425
              P     WCV   GV+   + G ++YAC QG   C PI  GG C+EP+++K HA++  
Sbjct: 370 TPTPKAAGSWCVPKPGVSDDQLTGNINYACGQG-IDCGPIQPGGACFEPNTVKAHAAYVM 429

Query: 426 SSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPS 461
           + Y+    +    C F+  AT T  +PSYG C FPS
Sbjct: 430 NLYYQSAGRNSWNCDFSQTATLTNTNPSYGACNFPS 457

[Close]

BLAST of Chy4G075310.1 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 320.1 bits (819), Expect = 4.2e-86
Identity = 175/460 (38.04%), Postives = 275/460 (59.78%), Query Frame = 0


Query: 6   LSLLFFLLFLS-LSSAEISTLVGINYGQLGNNLPSPSHSVNLIKSLNAQIVKIYDANPQI 65
           +S+ F L+FLS   S+     +G+NYGQ+ +NLP PS +V L++S + Q V++Y A+P I
Sbjct: 5   ISIYFLLIFLSHFPSSHAEPFIGVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGADPAI 64

Query: 66  LKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEIISST 125
           +KAL  T + + +   N  + +++S  N A QW+ +N+LPFYP +KI  + VGNEI+ S 
Sbjct: 65  IKALAGTGVGIVIGAANGDVPSLASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSN 124

Query: 126 GNQTWFSLVPAMRRIKHSLKTYGI-HKVKVGTPSAMDVLQSSFPPSNGTFRADIADRVMR 185
                  L+PAM+ ++ +L+   +  K+KV T ++M VL SS PPS+G+F A      ++
Sbjct: 125 DPNLVNQLLPAMQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGY-QTGLK 184

Query: 186 PMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLFDQM 245
            +LQFL+ T S F ++ YP+F + SD     L + LFE  N    D  +G+ YTN+FD  
Sbjct: 185 GILQFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFE-PNAGRVDSKTGIKYTNMFDAQ 244

Query: 246 VDSVIFAMKHLGFPEIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPPVGT 305
           VD+V  A+K +GF ++ + +AETGW + GD +++GA++ NA  YN N++  + S   VGT
Sbjct: 245 VDAVHSALKSMGFEKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSM--VGT 304

Query: 306 PARPGRVLPTIIFSLYNENEKPGPGTERHFGLLYPNGKAVYEMDLSGSTAETKFKPLPEA 365
           P  PG+ + T IF+LY+EN KPGP +ER FGL   +   VY++ L+ S++          
Sbjct: 305 PLMPGKPVDTYIFALYDENLKPGPSSERAFGLFKTDLSMVYDVGLAKSSSS--------- 364

Query: 366 ERNEPYKGKI----WCVVGKGVNMSDVVGALSYACSQGNKTCEPIGKGGPCYEPDSLKRH 425
             ++   GK+    WCV  KG    ++  +L +AC  G   C  I  GG C+EP+++  H
Sbjct: 365 --SQTPSGKVTSSGWCVPKKGATNEELQASLDWACGHG-IDCGAIQPGGACFEPNNVVSH 424

Query: 426 ASFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFP 460
           A++A + Y+ +  K    C F+  AT T ++PSY  C +P
Sbjct: 425 AAYAMNMYFQKSPKQPTDCDFSKTATVTSQNPSYNNCVYP 448

[Close]

BLAST of Chy4G075310.1 vs. ExPASy TrEMBL
Match: A0A0A0LBR1 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G634360 PE=3 SV=1)

HSP 1 Score: 930.2 bits (2403), Expect = 3.3e-267
Identity = 455/465 (97.85%), Postives = 459/465 (98.71%), Query Frame = 0


Query: 1   MPIPFLSLLFFLLFLSLSSAEISTLVGINYGQLGNNLPSPSHSVNLIKSLNAQIVKIYDA 60
           MPIPFLSLLFFL FLSLSSAEISTLVGINYGQLGNNLP PSHSVNLIKSLNAQIVKIYDA
Sbjct: 1   MPIPFLSLLFFLFFLSLSSAEISTLVGINYGQLGNNLPPPSHSVNLIKSLNAQIVKIYDA 60

Query: 61  NPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEI 120
           NPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEI
Sbjct: 61  NPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEI 120

Query: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADR 180
           ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADR
Sbjct: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADR 180

Query: 181 VMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240
           VMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF
Sbjct: 181 VMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240

Query: 241 DQMVDSVIFAMKHLGFPEIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPP 300
           DQMVDSVIFAMK LGFP+IRVFIAETGWPNGGDYDQIGA+IHNAAVYNRNIVKRVTSKPP
Sbjct: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGASIHNAAVYNRNIVKRVTSKPP 300

Query: 301 VGTPARPGRVLPTIIFSLYNENEKPGPGTERHFGLLYPNGKAVYEMDLSGSTAETKFKPL 360
           VGTPARPGR+LPTIIFSLYNEN+KPGPGTERHFGLLYP GKAVYEMDLSGS AETKFKPL
Sbjct: 301 VGTPARPGRMLPTIIFSLYNENQKPGPGTERHFGLLYPKGKAVYEMDLSGSRAETKFKPL 360

Query: 361 PEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIGKGGPCYEPDSLKRHA 420
           PEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPI KGGPCYEPDSLKRHA
Sbjct: 361 PEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQA 466
           SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQA
Sbjct: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQA 465

[Close]

BLAST of Chy4G075310.1 vs. ExPASy TrEMBL
Match: A0A5A7VIC4 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold238G00460 PE=3 SV=1)

HSP 1 Score: 909.1 bits (2348), Expect = 7.8e-261
Identity = 444/464 (95.69%), Postives = 453/464 (97.63%), Query Frame = 0


Query: 1   MPIPFLSLLFFLLFLSLSSAEISTLVGINYGQLGNNLPSPSHSVNLIKSLNAQIVKIYDA 60
           MPIPFLSLL F  FLSLSSAEIS+LVGINYGQLGNNLPSPSHSVNLIKSLNAQIVKIYDA
Sbjct: 1   MPIPFLSLLLFPFFLSLSSAEISSLVGINYGQLGNNLPSPSHSVNLIKSLNAQIVKIYDA 60

Query: 61  NPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEI 120
           NPQIL ALKNTDLRVSVMVPNELIINISS+QNLADQWVRTNILPFYPQ KIRYLLVGNEI
Sbjct: 61  NPQILNALKNTDLRVSVMVPNELIINISSNQNLADQWVRTNILPFYPQIKIRYLLVGNEI 120

Query: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADR 180
           ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGT SAMDVLQSSFPPSNGTFR DIADR
Sbjct: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTSSAMDVLQSSFPPSNGTFREDIADR 180

Query: 181 VMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240
           VMRPMLQFLNRTKSF+FLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF
Sbjct: 181 VMRPMLQFLNRTKSFYFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240

Query: 241 DQMVDSVIFAMKHLGFPEIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPP 300
           DQMVDSVIFAMK LGFP+IRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKR+TSKPP
Sbjct: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRMTSKPP 300

Query: 301 VGTPARPGRVLPTIIFSLYNENEKPGPGTERHFGLLYPNGKAVYEMDLSGSTAETKFKPL 360
           VGTPARPGRVLPTIIFSLYNENEK GPGTERHFGLLYP+GKAVYEMDLSGSTAETKFKPL
Sbjct: 301 VGTPARPGRVLPTIIFSLYNENEKGGPGTERHFGLLYPSGKAVYEMDLSGSTAETKFKPL 360

Query: 361 PEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIGKGGPCYEPDSLKRHA 420
           PEAE NEPYKG+IWCVVGKGVNMS+VVGALSYACSQGNKTCEPI KGGPCYEPDSLKRHA
Sbjct: 361 PEAETNEPYKGRIWCVVGKGVNMSEVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ 465
           SFAFSSYWAQFRK+GGTCYFNGLATQTIKDPSYGKCKFPSVTLQ
Sbjct: 421 SFAFSSYWAQFRKIGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ 464

[Close]

BLAST of Chy4G075310.1 vs. ExPASy TrEMBL
Match: A0A1S3CCE3 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103499229 PE=3 SV=1)

HSP 1 Score: 909.1 bits (2348), Expect = 7.8e-261
Identity = 444/464 (95.69%), Postives = 453/464 (97.63%), Query Frame = 0


Query: 1   MPIPFLSLLFFLLFLSLSSAEISTLVGINYGQLGNNLPSPSHSVNLIKSLNAQIVKIYDA 60
           MPIPFLSLL F  FLSLSSAEIS+LVGINYGQLGNNLPSPSHSVNLIKSLNAQIVKIYDA
Sbjct: 1   MPIPFLSLLLFPFFLSLSSAEISSLVGINYGQLGNNLPSPSHSVNLIKSLNAQIVKIYDA 60

Query: 61  NPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEI 120
           NPQIL ALKNTDLRVSVMVPNELIINISS+QNLADQWVRTNILPFYPQ KIRYLLVGNEI
Sbjct: 61  NPQILNALKNTDLRVSVMVPNELIINISSNQNLADQWVRTNILPFYPQIKIRYLLVGNEI 120

Query: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADR 180
           ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGT SAMDVLQSSFPPSNGTFR DIADR
Sbjct: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTSSAMDVLQSSFPPSNGTFREDIADR 180

Query: 181 VMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240
           VMRPMLQFLNRTKSF+FLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF
Sbjct: 181 VMRPMLQFLNRTKSFYFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240

Query: 241 DQMVDSVIFAMKHLGFPEIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPP 300
           DQMVDSVIFAMK LGFP+IRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKR+TSKPP
Sbjct: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRMTSKPP 300

Query: 301 VGTPARPGRVLPTIIFSLYNENEKPGPGTERHFGLLYPNGKAVYEMDLSGSTAETKFKPL 360
           VGTPARPGRVLPTIIFSLYNENEK GPGTERHFGLLYP+GKAVYEMDLSGSTAETKFKPL
Sbjct: 301 VGTPARPGRVLPTIIFSLYNENEKGGPGTERHFGLLYPSGKAVYEMDLSGSTAETKFKPL 360

Query: 361 PEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIGKGGPCYEPDSLKRHA 420
           PEAE NEPYKG+IWCVVGKGVNMS+VVGALSYACSQGNKTCEPI KGGPCYEPDSLKRHA
Sbjct: 361 PEAETNEPYKGRIWCVVGKGVNMSEVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ 465
           SFAFSSYWAQFRK+GGTCYFNGLATQTIKDPSYGKCKFPSVTLQ
Sbjct: 421 SFAFSSYWAQFRKIGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ 464

[Close]

BLAST of Chy4G075310.1 vs. ExPASy TrEMBL
Match: A0A6J1EXC8 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111437372 PE=3 SV=1)

HSP 1 Score: 830.9 bits (2145), Expect = 2.7e-237
Identity = 403/464 (86.85%), Postives = 427/464 (92.03%), Query Frame = 0


Query: 1   MPIPFLSLLFFLLFLSLSSAEISTLVGINYGQLGNNLPSPSHSVNLIKSLNAQIVKIYDA 60
           M IPF SLL    FLSL+SA+ISTLVGINYGQLGNNLP P  SV+LIKSLNAQIVKIYD 
Sbjct: 1   MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV 60

Query: 61  NPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEI 120
           NPQILK+L N+D++VSVMVPN+LIINISS+QNLAD WVRTNILPFYPQ KIRYLLVGNEI
Sbjct: 61  NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI 120

Query: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADR 180
           ISSTGNQTW SLVPAMRRIK SLKTYGIHKVKVGT SAMDVLQSSFPPSNGTFRADI+DR
Sbjct: 121 ISSTGNQTWISLVPAMRRIKRSLKTYGIHKVKVGTSSAMDVLQSSFPPSNGTFRADISDR 180

Query: 181 VMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240
           VM+PMLQFLNRTKSFFFLDVYP+FPW+SD VNIKLDYALFESKNITYSDP SGL YTNLF
Sbjct: 181 VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF 240

Query: 241 DQMVDSVIFAMKHLGFPEIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPP 300
           DQMVDSVIFAMK LG+P+IR+FIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVT+KPP
Sbjct: 241 DQMVDSVIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP 300

Query: 301 VGTPARPGRVLPTIIFSLYNENEKPGPGTERHFGLLYPNGKAVYEMDLSGSTAETKFKPL 360
           VGTPARPG VLPTIIFSLYNEN+KPG GTERHFGLLYPN  AVYEMDLSG T E  FKPL
Sbjct: 301 VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL 360

Query: 361 PEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIGKGGPCYEPDSLKRHA 420
           P AE NEPYKGKIWC+V +G N S+V GAL++AC QGNKTCEPI KGGPCYEPDSL RHA
Sbjct: 361 PAAETNEPYKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ 465
           SFAFSSYWAQFRKVGGTCYFNGLATQTIKDP+YGKCKFPSVTLQ
Sbjct: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPNYGKCKFPSVTLQ 464

[Close]

BLAST of Chy4G075310.1 vs. ExPASy TrEMBL
Match: A0A6J1K875 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491531 PE=3 SV=1)

HSP 1 Score: 823.2 bits (2125), Expect = 5.6e-235
Identity = 399/464 (85.99%), Postives = 425/464 (91.59%), Query Frame = 0


Query: 1   MPIPFLSLLFFLLFLSLSSAEISTLVGINYGQLGNNLPSPSHSVNLIKSLNAQIVKIYDA 60
           M IPF SLL    FLSL+SA+ISTLVGINYGQLGNNLP P  SV+LIKSLNAQIVKIYD 
Sbjct: 1   MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV 60

Query: 61  NPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEI 120
           NPQILK+L N+D++VSVMVPN+LIINISS+QNLAD WVRTNILPFYPQ KIRYLLVGNEI
Sbjct: 61  NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI 120

Query: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADR 180
           ISSTGNQTW SLVPAMRRIK SLK YGIHKVKVG+ SAMD+LQSSFPPSNGTFRADI+DR
Sbjct: 121 ISSTGNQTWISLVPAMRRIKRSLKIYGIHKVKVGSSSAMDILQSSFPPSNGTFRADISDR 180

Query: 181 VMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240
           VM+PMLQFLNRTKSFFFLDVYP+FPW+SD VNIKLDYALFESKNITYSDP SGL YTNLF
Sbjct: 181 VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF 240

Query: 241 DQMVDSVIFAMKHLGFPEIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPP 300
           DQMVDSVIFAMK LG+P+IRVFI+ETGWPNGGDYDQIGANIHNAAVYNRNIVKRVT+KPP
Sbjct: 241 DQMVDSVIFAMKRLGYPDIRVFISETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP 300

Query: 301 VGTPARPGRVLPTIIFSLYNENEKPGPGTERHFGLLYPNGKAVYEMDLSGSTAETKFKPL 360
           VGTPARPG VLPTIIFSLYNEN+KPG GTERHFGLLYPN  AVYEMDLSG T E  FKPL
Sbjct: 301 VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL 360

Query: 361 PEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIGKGGPCYEPDSLKRHA 420
           P AE NEP+KGKIWC+V +G N S+V GAL++AC QGNKTCEPI KGGPCYEPDSL RHA
Sbjct: 361 PAAETNEPFKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ 465
           SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKC FPSVTLQ
Sbjct: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCMFPSVTLQ 464

[Close]

BLAST of Chy4G075310.1 vs. NCBI nr
Match: XP_004144397.1 (probable glucan endo-1,3-beta-glucosidase A6 [Cucumis sativus] >KGN58384.1 hypothetical protein Csa_001975 [Cucumis sativus])

HSP 1 Score: 924 bits (2389), Expect = 0.0
Identity = 455/465 (97.85%), Postives = 459/465 (98.71%), Query Frame = 0


Query: 1   MPIPFLSLLFFLLFLSLSSAEISTLVGINYGQLGNNLPSPSHSVNLIKSLNAQIVKIYDA 60
           MPIPFLSLLFFL FLSLSSAEISTLVGINYGQLGNNLP PSHSVNLIKSLNAQIVKIYDA
Sbjct: 1   MPIPFLSLLFFLFFLSLSSAEISTLVGINYGQLGNNLPPPSHSVNLIKSLNAQIVKIYDA 60

Query: 61  NPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEI 120
           NPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEI
Sbjct: 61  NPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEI 120

Query: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADR 180
           ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADR
Sbjct: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADR 180

Query: 181 VMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240
           VMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF
Sbjct: 181 VMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240

Query: 241 DQMVDSVIFAMKHLGFPEIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPP 300
           DQMVDSVIFAMK LGFP+IRVFIAETGWPNGGDYDQIGA+IHNAAVYNRNIVKRVTSKPP
Sbjct: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGASIHNAAVYNRNIVKRVTSKPP 300

Query: 301 VGTPARPGRVLPTIIFSLYNENEKPGPGTERHFGLLYPNGKAVYEMDLSGSTAETKFKPL 360
           VGTPARPGR+LPTIIFSLYNEN+KPGPGTERHFGLLYP GKAVYEMDLSGS AETKFKPL
Sbjct: 301 VGTPARPGRMLPTIIFSLYNENQKPGPGTERHFGLLYPKGKAVYEMDLSGSRAETKFKPL 360

Query: 361 PEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIGKGGPCYEPDSLKRHA 420
           PEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPI KGGPCYEPDSLKRHA
Sbjct: 361 PEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQA 465
           SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQA
Sbjct: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQA 465

[Close]

BLAST of Chy4G075310.1 vs. NCBI nr
Match: XP_008460404.1 (PREDICTED: probable glucan endo-1,3-beta-glucosidase A6 [Cucumis melo] >KAA0068092.1 putative glucan endo-1,3-beta-glucosidase A6 [Cucumis melo var. makuwa])

HSP 1 Score: 904 bits (2335), Expect = 0.0
Identity = 444/464 (95.69%), Postives = 453/464 (97.63%), Query Frame = 0


Query: 1   MPIPFLSLLFFLLFLSLSSAEISTLVGINYGQLGNNLPSPSHSVNLIKSLNAQIVKIYDA 60
           MPIPFLSLL F  FLSLSSAEIS+LVGINYGQLGNNLPSPSHSVNLIKSLNAQIVKIYDA
Sbjct: 1   MPIPFLSLLLFPFFLSLSSAEISSLVGINYGQLGNNLPSPSHSVNLIKSLNAQIVKIYDA 60

Query: 61  NPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEI 120
           NPQIL ALKNTDLRVSVMVPNELIINISS+QNLADQWVRTNILPFYPQ KIRYLLVGNEI
Sbjct: 61  NPQILNALKNTDLRVSVMVPNELIINISSNQNLADQWVRTNILPFYPQIKIRYLLVGNEI 120

Query: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADR 180
           ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGT SAMDVLQSSFPPSNGTFR DIADR
Sbjct: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTSSAMDVLQSSFPPSNGTFREDIADR 180

Query: 181 VMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240
           VMRPMLQFLNRTKSF+FLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF
Sbjct: 181 VMRPMLQFLNRTKSFYFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240

Query: 241 DQMVDSVIFAMKHLGFPEIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPP 300
           DQMVDSVIFAMK LGFP+IRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKR+TSKPP
Sbjct: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRMTSKPP 300

Query: 301 VGTPARPGRVLPTIIFSLYNENEKPGPGTERHFGLLYPNGKAVYEMDLSGSTAETKFKPL 360
           VGTPARPGRVLPTIIFSLYNENEK GPGTERHFGLLYP+GKAVYEMDLSGSTAETKFKPL
Sbjct: 301 VGTPARPGRVLPTIIFSLYNENEKGGPGTERHFGLLYPSGKAVYEMDLSGSTAETKFKPL 360

Query: 361 PEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIGKGGPCYEPDSLKRHA 420
           PEAE NEPYKG+IWCVVGKGVNMS+VVGALSYACSQGNKTCEPI KGGPCYEPDSLKRHA
Sbjct: 361 PEAETNEPYKGRIWCVVGKGVNMSEVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ 464
           SFAFSSYWAQFRK+GGTCYFNGLATQTIKDPSYGKCKFPSVTLQ
Sbjct: 421 SFAFSSYWAQFRKIGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ 464

[Close]

BLAST of Chy4G075310.1 vs. NCBI nr
Match: XP_038886980.1 (probable glucan endo-1,3-beta-glucosidase A6 [Benincasa hispida])

HSP 1 Score: 867 bits (2240), Expect = 0.0
Identity = 425/464 (91.59%), Postives = 440/464 (94.83%), Query Frame = 0


Query: 1   MPIPFLSLLFFLLFLSLSSAEISTLVGINYGQLGNNLPSPSHSVNLIKSLNAQIVKIYDA 60
           M IPFLSLL    FLSLSSAEIS+LVGINYGQLGNNLP PS SVNLIKSLNAQIVKIYDA
Sbjct: 1   MAIPFLSLLLPFFFLSLSSAEISSLVGINYGQLGNNLPPPSDSVNLIKSLNAQIVKIYDA 60

Query: 61  NPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEI 120
           NPQILK LKNTDLRVSVMVPN+LIINISS+QNLAD WVRTN+ PFYPQTKIRYLLVGNEI
Sbjct: 61  NPQILKTLKNTDLRVSVMVPNDLIINISSNQNLADDWVRTNVRPFYPQTKIRYLLVGNEI 120

Query: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADR 180
           +SSTGN+TWFSLVPAMRRIK SLKTYGI KVKVGT SAMDVLQSSFPPSNGTFRADIA+R
Sbjct: 121 VSSTGNETWFSLVPAMRRIKRSLKTYGIRKVKVGTSSAMDVLQSSFPPSNGTFRADIAER 180

Query: 181 VMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240
           VM+PMLQFLNRTKSFFFLDVYPYFPW+ DSVNIKLDYALFESKNITYSDP SGL+YTNLF
Sbjct: 181 VMKPMLQFLNRTKSFFFLDVYPYFPWAIDSVNIKLDYALFESKNITYSDPGSGLIYTNLF 240

Query: 241 DQMVDSVIFAMKHLGFPEIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPP 300
           DQMVDSVIFAMK LGFP++RVFIAETGWPNGGDYDQIGA+IHNAAVYNRNIVKRVTSKPP
Sbjct: 241 DQMVDSVIFAMKRLGFPDVRVFIAETGWPNGGDYDQIGASIHNAAVYNRNIVKRVTSKPP 300

Query: 301 VGTPARPGRVLPTIIFSLYNENEKPGPGTERHFGLLYPNGKAVYEMDLSGSTAETKFKPL 360
           VGTPARPGRVLPTIIFSLYNEN+KPGPGTERHFGLLYPN  AVYEMDLSGST ET+FKPL
Sbjct: 301 VGTPARPGRVLPTIIFSLYNENQKPGPGTERHFGLLYPNRTAVYEMDLSGSTPETEFKPL 360

Query: 361 PEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIGKGGPCYEPDSLKRHA 420
           P AE NEPYKGKIWCVVGKG NMSDVVGALSYACSQGNKTCEPI KGGPCYEPDSLKRHA
Sbjct: 361 PAAETNEPYKGKIWCVVGKGANMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ 464
           SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSV LQ
Sbjct: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVVLQ 464

[Close]

BLAST of Chy4G075310.1 vs. NCBI nr
Match: XP_022931078.1 (probable glucan endo-1,3-beta-glucosidase A6 [Cucurbita moschata])

HSP 1 Score: 825 bits (2132), Expect = 5.88e-300
Identity = 403/464 (86.85%), Postives = 427/464 (92.03%), Query Frame = 0


Query: 1   MPIPFLSLLFFLLFLSLSSAEISTLVGINYGQLGNNLPSPSHSVNLIKSLNAQIVKIYDA 60
           M IPF SLL    FLSL+SA+ISTLVGINYGQLGNNLP P  SV+LIKSLNAQIVKIYD 
Sbjct: 1   MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV 60

Query: 61  NPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEI 120
           NPQILK+L N+D++VSVMVPN+LIINISS+QNLAD WVRTNILPFYPQ KIRYLLVGNEI
Sbjct: 61  NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI 120

Query: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADR 180
           ISSTGNQTW SLVPAMRRIK SLKTYGIHKVKVGT SAMDVLQSSFPPSNGTFRADI+DR
Sbjct: 121 ISSTGNQTWISLVPAMRRIKRSLKTYGIHKVKVGTSSAMDVLQSSFPPSNGTFRADISDR 180

Query: 181 VMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240
           VM+PMLQFLNRTKSFFFLDVYP+FPW+SD VNIKLDYALFESKNITYSDP SGL YTNLF
Sbjct: 181 VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF 240

Query: 241 DQMVDSVIFAMKHLGFPEIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPP 300
           DQMVDSVIFAMK LG+P+IR+FIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVT+KPP
Sbjct: 241 DQMVDSVIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP 300

Query: 301 VGTPARPGRVLPTIIFSLYNENEKPGPGTERHFGLLYPNGKAVYEMDLSGSTAETKFKPL 360
           VGTPARPG VLPTIIFSLYNEN+KPG GTERHFGLLYPN  AVYEMDLSG T E  FKPL
Sbjct: 301 VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL 360

Query: 361 PEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIGKGGPCYEPDSLKRHA 420
           P AE NEPYKGKIWC+V +G N S+V GAL++AC QGNKTCEPI KGGPCYEPDSL RHA
Sbjct: 361 PAAETNEPYKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ 464
           SFAFSSYWAQFRKVGGTCYFNGLATQTIKDP+YGKCKFPSVTLQ
Sbjct: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPNYGKCKFPSVTLQ 464

[Close]

BLAST of Chy4G075310.1 vs. NCBI nr
Match: XP_023532592.1 (probable glucan endo-1,3-beta-glucosidase A6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 824 bits (2129), Expect = 1.69e-299
Identity = 403/464 (86.85%), Postives = 426/464 (91.81%), Query Frame = 0


Query: 1   MPIPFLSLLFFLLFLSLSSAEISTLVGINYGQLGNNLPSPSHSVNLIKSLNAQIVKIYDA 60
           M IPF SLL    FLSL+SA+ISTLVGINYGQLGNNLP P  SV+LIKSLNAQIVKIYD 
Sbjct: 1   MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV 60

Query: 61  NPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEI 120
           NPQILK+L N+D++VSVMVPN+LIINISS+QNLAD WVRTNILPFYPQ KIRYLLVGNEI
Sbjct: 61  NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI 120

Query: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADR 180
           ISSTGNQTW SLVPAMRRIK SLK YGIHKVKVGT SAMDVLQSSFPPSNGTFRADI+DR
Sbjct: 121 ISSTGNQTWISLVPAMRRIKRSLKIYGIHKVKVGTSSAMDVLQSSFPPSNGTFRADISDR 180

Query: 181 VMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240
           VM+PMLQFLNRTKSFFFLDVYP+FPW+SD VNIKLDYALFESKNITYSDP SGL YTNLF
Sbjct: 181 VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF 240

Query: 241 DQMVDSVIFAMKHLGFPEIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPP 300
           DQMVDSVIFAMK LG+P+IR+FIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVT+KPP
Sbjct: 241 DQMVDSVIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP 300

Query: 301 VGTPARPGRVLPTIIFSLYNENEKPGPGTERHFGLLYPNGKAVYEMDLSGSTAETKFKPL 360
           VGTPARPG VLPTIIFSLYNEN+KPG GTERHFGLLYPN  AVYEMDLSG T E  FKPL
Sbjct: 301 VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL 360

Query: 361 PEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIGKGGPCYEPDSLKRHA 420
           P AE NEPYKGKIWC+V +G N S+V GAL++AC QGNKTCEPI KGGPCYEPDSL RHA
Sbjct: 361 PAAETNEPYKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ 464
           SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ
Sbjct: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ 464

[Close]

BLAST of Chy4G075310.1 vs. TAIR 10
Match: AT3G07320.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 657.5 bits (1695), Expect = 7.9e-189
Identity = 313/458 (68.34%), Postives = 375/458 (81.88%), Query Frame = 0


Query: 6   LSLLFFLLFLSLSSAEISTLVGINYGQLGNNLPSPSHSVNLIKSLNAQIVKIYDANPQIL 65
           L L    L +S+S A+ S   GINYGQLGNNLPSPS SVNLIKSLNA+ VK+YDANP+IL
Sbjct: 5   LHLFALSLLISVSGAKFSGRPGINYGQLGNNLPSPSDSVNLIKSLNAKRVKLYDANPKIL 64

Query: 66  KALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEIISSTG 125
            AL  TD+ VSVMVPNEL++NIS S +L+D W+R+NILPFYP TKIRYLLVGNEI+S   
Sbjct: 65  AALNGTDITVSVMVPNELLVNISKSASLSDDWIRSNILPFYPTTKIRYLLVGNEILSLPD 124

Query: 126 NQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADRVMRPM 185
           ++   SLVPAMR+I+ SLK+ G+ KVKVGT  A DVLQSSFPPS+G FR DI+  +M+PM
Sbjct: 125 SELKSSLVPAMRKIQRSLKSLGVKKVKVGTTLATDVLQSSFPPSSGEFREDISGLIMKPM 184

Query: 186 LQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLFDQMVD 245
           LQFLNRTKSF F+DVYPYF W+ D  ++ LDYA+FES N+T +DPVS L Y NLFDQM+D
Sbjct: 185 LQFLNRTKSFLFVDVYPYFAWAQDPTHVDLDYAIFESTNVTVTDPVSNLTYHNLFDQMID 244

Query: 246 SVIFAMKHLGFPEIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPPVGTPA 305
           + +FAMK +G+P+IR+++AETGWPN GDYDQIGANI+NAA YNRN+VK++ + PPVGTPA
Sbjct: 245 AFVFAMKRVGYPDIRIWVAETGWPNNGDYDQIGANIYNAATYNRNVVKKLAADPPVGTPA 304

Query: 306 RPGRVLPTIIFSLYNENEKPGPGTERHFGLLYPNGKAVYEMDLSGSTAETKFKPLPEAER 365
           RPG+VLP  +F+LYNEN+K GPGTERHFGLL+PNG  VY +DLSG T E K + LP  E 
Sbjct: 305 RPGKVLPAFVFALYNENQKTGPGTERHFGLLHPNGTQVYGIDLSGKT-EYK-ESLPAPEN 364

Query: 366 NEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIGKGGPCYEPDSLKRHASFAFS 425
           N+ YKGKIWCVV KG N + +  ALSYACSQGN TC+PI +GGPC +PD    HAS+AFS
Sbjct: 365 NDLYKGKIWCVVAKGANWTQLGDALSYACSQGNNTCDPIQRGGPCQKPDLTVLHASYAFS 424

Query: 426 SYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTL 464
           SYWAQFRK+GGTC FNGLATQTIKDPSYG+C+FPSVTL
Sbjct: 425 SYWAQFRKIGGTCSFNGLATQTIKDPSYGRCEFPSVTL 460

[Close]

BLAST of Chy4G075310.1 vs. TAIR 10
Match: AT3G23770.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 553.9 bits (1426), Expect = 1.2e-157
Identity = 267/475 (56.21%), Postives = 346/475 (72.84%), Query Frame = 0


Query: 4   PFLSLLFFLLFLSLSSAE------------ISTLVGINYGQLGNNLPSPSHSVNLIKSLN 63
           PF   LF LL LS S               +++ +GINYG+LGNNLP P  S+NLIK++ 
Sbjct: 3   PFALFLFTLLALSSSCCSAIGPQNNRTVLALASRIGINYGKLGNNLPFPYQSINLIKTIK 62

Query: 64  AQIVKIYDANPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKI 123
           A  VK+YDA+P+ LK L  T+L V++MVPN  II+I + Q  AD WV TN+LPF+PQT+I
Sbjct: 63  AGHVKLYDADPETLKLLSTTNLYVTIMVPNNQIISIGADQAAADNWVATNVLPFHPQTRI 122

Query: 124 RYLLVGNEIISSTGN---QTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPP 183
           R++LVGNE++S + +   Q W +LVPAMR++ +SL+  GIH +KVGTP AMD L+SSFPP
Sbjct: 123 RFVLVGNEVLSYSSDQDKQIWANLVPAMRKVVNSLRARGIHNIKVGTPLAMDALRSSFPP 182

Query: 184 SNGTFRADIADRVMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYS 243
           S+GTFR DIA  VM P+L+FLN T SFFFLDVYPYFPWS+D VN  LD+ALFES N TY+
Sbjct: 183 SSGTFREDIAVPVMLPLLKFLNGTNSFFFLDVYPYFPWSTDPVNNHLDFALFES-NSTYT 242

Query: 244 DPVSGLVYTNLFDQMVDSVIFAMKHLGFPEIRVFIAETGWPNGGDYDQIGANIHNAAVYN 303
           DP +GLVYTNL DQM+DSVIFAM  LG+P I + I+ETGWPN GD  + GANI NAA YN
Sbjct: 243 DPQTGLVYTNLLDQMLDSVIFAMTKLGYPNISLAISETGWPNDGDIHETGANIVNAATYN 302

Query: 304 RNIVKRVTSKPPVGTPARPGRVLPTIIFSLYNENEKPGPGTERHFGLLYPNGKAVYEMDL 363
           RN++K++T+ PP+GTPAR G  +PT +FSL+NEN+KPG GTERH+G+L P+G  +Y++D 
Sbjct: 303 RNLIKKMTANPPLGTPARRGAPIPTFLFSLFNENQKPGSGTERHWGILNPDGTPIYDIDF 362

Query: 364 SGSTAETKFKPLPEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIGKGG 423
           SG  + + F  LP+   N P+KG +WCV   G + +++  AL++AC + N TC  +  GG
Sbjct: 363 SGRRSFSGFDSLPKPSNNVPFKGNVWCVAVDGADEAELGQALNFACGRSNATCAALAPGG 422

Query: 424 PCYEPDSLKRHASFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTL 464
            CY P ++  HAS+AFSSYWAQFR     CYFNGLA +T  +P   +CKFPSVTL
Sbjct: 423 ECYAPVTVTWHASYAFSSYWAQFRNQSSQCYFNGLARETTTNPGNERCKFPSVTL 476

[Close]

BLAST of Chy4G075310.1 vs. TAIR 10
Match: AT4G14080.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 539.3 bits (1388), Expect = 3.1e-153
Identity = 246/443 (55.53%), Postives = 333/443 (75.17%), Query Frame = 0


Query: 21  EISTLVGINYGQLGNNLPSPSHSVNLIKSLNAQIVKIYDANPQILKALKNTDLRVSVMVP 80
           ++++ +GINYG+ GNNLPSP  S+N IKS+ A  VK+YDA+P+ L  L  T+L V++ VP
Sbjct: 37  DLASKIGINYGRRGNNLPSPYQSINFIKSIKAGHVKLYDADPESLTLLSQTNLYVTITVP 96

Query: 81  NELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEIISSTGNQTWFSLVPAMRRIK 140
           N  I  +SS+Q +AD+WVRTNILP+YPQT+IR++LVGNEI+S        +LVPAMR+I 
Sbjct: 97  NHQITALSSNQTIADEWVRTNILPYYPQTQIRFVLVGNEILSYNSGNVSVNLVPAMRKIV 156

Query: 141 HSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADRVMRPMLQFLNRTKSFFFLDV 200
           +SL+ +GIH +KVGTP AMD L+SSFPPSNGTFR +I   VM P+L+FLN T S+FFL+V
Sbjct: 157 NSLRLHGIHNIKVGTPLAMDSLRSSFPPSNGTFREEITGPVMLPLLKFLNGTNSYFFLNV 216

Query: 201 YPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLFDQMVDSVIFAMKHLGFPEIR 260
           +PYF WS + +N  LD+ALF+  + TY+DP +GLVY NL DQM+DSV+FAM  LG+P +R
Sbjct: 217 HPYFRWSRNPMNTSLDFALFQGHS-TYTDPQTGLVYRNLLDQMLDSVLFAMTKLGYPHMR 276

Query: 261 VFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPPVGTPARPGRVLPTIIFSLYN 320
           + I+ETGWPN GD D+ GANI NAA YNRN++K++++ PP+GTP+RPG  +PT +FSL+N
Sbjct: 277 LAISETGWPNFGDIDETGANILNAATYNRNLIKKMSASPPIGTPSRPGLPIPTFVFSLFN 336

Query: 321 ENEKPGPGTERHFGLLYPNGKAVYEMDLSGSTAETKFKPLPEAERNEPYKGKIWCVVGKG 380
           EN+K G GT+RH+G+L+P+G  +Y++D +G T  T F PLP+   N PYKG++WCV  +G
Sbjct: 337 ENQKSGSGTQRHWGILHPDGSPIYDVDFTGQTPLTGFNPLPKPTNNVPYKGQVWCVPVEG 396

Query: 381 VNMSDVVGALSYACSQGNKTCEPIGKGGPCYEPDSLKRHASFAFSSYWAQFRKVGGTCYF 440
            N +++   L  AC+Q N TC  +  G  CYEP S+  HAS+A +SYWAQFR     C+F
Sbjct: 397 ANETELEETLRMACAQSNTTCAALAPGRECYEPVSIYWHASYALNSYWAQFRNQSIQCFF 456

Query: 441 NGLATQTIKDPSYGKCKFPSVTL 464
           NGLA +T  +P   +CKFPSVTL
Sbjct: 457 NGLAHETTTNPGNDRCKFPSVTL 478

[Close]

BLAST of Chy4G075310.1 vs. TAIR 10
Match: AT4G26830.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 365.9 bits (938), Expect = 4.7e-101
Identity = 188/463 (40.60%), Postives = 285/463 (61.56%), Query Frame = 0


Query: 1   MPIPFLSLLFFLLFLSLSSAEISTLVGINYGQLGNNLPSPSHSVNLIKSLNAQIVKIYDA 60
           M + FL     L FLS   A  S +VG+NYG++ NNLPSP   VNL+KS     +KI+D 
Sbjct: 1   MAVSFLPYFLILSFLSAIDAH-SGMVGVNYGRIANNLPSPEKVVNLLKSQGINRIKIFDT 60

Query: 61  NPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEI 120
           +  +L AL N+ ++V V +PNEL+ + +S Q+ AD W++T+I+P++P T+I  + VGNE+
Sbjct: 61  DKNVLTALANSKIKVIVALPNELLSSAASHQSFADNWIKTHIMPYFPATEIEAIAVGNEV 120

Query: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHK-VKVGTPSAMDVLQSSFPPSNGTFRADIAD 180
                   +  LV AM+ I  SL  Y + K +K+ +P A+  L +S+PPS+G+F+ ++ +
Sbjct: 121 FVDPTITPY--LVNAMKNIHTSLVKYKLDKAIKISSPIALSALANSYPPSSGSFKPELIE 180

Query: 181 RVMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNL 240
            V++PML  L +T S+  ++ YP+F +++++  I LDYALF+ +N    D  +GL Y +L
Sbjct: 181 PVVKPMLALLQQTSSYLMVNAYPFFAYAANADKISLDYALFK-ENAGNIDSGTGLKYNSL 240

Query: 241 FDQMVDSVIFAMKHLGFPEIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKP 300
           FD  +D+V  A+  +GF  ++V + ETGWP+ GD ++IGA+  NAA YN  +VKRV +  
Sbjct: 241 FDAQIDAVYAALSAVGFKGVKVMVTETGWPSVGDENEIGASESNAAAYNAGLVKRVLTGK 300

Query: 301 PVGTPARPGRVLPTIIFSLYNENEKPGPGTERHFGLLYPNGKAVYEMDLSGSTAETKFKP 360
             GTP RP   L   +F+L+NEN+KPGP +ER++GL YPN   VY +  +  +      P
Sbjct: 301 --GTPLRPTEPLNVYLFALFNENQKPGPTSERNYGLFYPNEGKVYNVPFTKKST----TP 360

Query: 361 LPEAERNEP--YKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIGKGGPCYEPDSLK 420
           +       P  ++G  WCV    V    +  AL YAC +G   C PI  G  CY P+SL+
Sbjct: 361 VNGNRGKVPVTHEGHTWCVSNGEVAKEKLQEALDYACGEGGADCRPIQPGATCYHPESLE 420

Query: 421 RHASFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPS 461
            HAS+AF+SY+ +  +  GTC+F G A    + P YGKC+FP+
Sbjct: 421 AHASYAFNSYYQKNSRRVGTCFFGGAAHVVTQPPRYGKCEFPT 453

[Close]

BLAST of Chy4G075310.1 vs. TAIR 10
Match: AT2G05790.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 363.2 bits (931), Expect = 3.1e-100
Identity = 186/468 (39.74%), Postives = 281/468 (60.04%), Query Frame = 0


Query: 8   LLFFLLFLSLSSAEISTLVGINYGQLGNNLPSPSHSVNLIKSLNAQIVKIYDANPQILKA 67
           L+F LL LS  S   +  +G+NYG++ + LPS    V L+KS     VKI+DA+P +LKA
Sbjct: 7   LIFLLLLLSPFSLSDAGSIGVNYGRISDELPSAFKVVQLLKSQGITRVKIFDADPSVLKA 66

Query: 68  LKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEIISSTGNQ 127
           L  + ++V+V +PNEL+ + +   + A  WV+ N+  ++P T+I  + VGNE+   T N 
Sbjct: 67  LSGSGIKVTVDLPNELLFSAAKRTSFAVSWVKRNVAAYHPSTQIESIAVGNEVFVDTHNT 126

Query: 128 TWFSLVPAMRRIKHSLKTYGIHK-VKVGTPSAMDVLQSSFPPSNGTFRADIADRVMRPML 187
           T F L+PAMR I  +L ++ +H  +K+ +P A+  LQ+S+P S+G+FR ++ D V++PML
Sbjct: 127 TSF-LIPAMRNIHKALMSFNLHSDIKISSPLALSALQNSYPSSSGSFRPELIDSVIKPML 186

Query: 188 QFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLFDQMVDS 247
            FL  T S   ++VYP+F +  +S  I LDYAL   +N    D  +GL Y NLFD  +D+
Sbjct: 187 DFLRETGSRLMINVYPFFAYEGNSDVIPLDYALLR-ENPGMVDSGNGLRYFNLFDAQIDA 246

Query: 248 VIFAMKHLGFPEIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPPVGTPAR 307
           V  AM  L + +I + + ETGWP+ GD +++GA + NAA YN N+++R+ ++   GTP R
Sbjct: 247 VFAAMSALKYDDIEIIVTETGWPSKGDENEVGATLANAASYNGNLIRRILTRG--GTPLR 306

Query: 308 PGRVLPTIIFSLYNENEKPGPGTERHFGLLYPNGKAVYEMDLS----------GSTAET- 367
           P   L   +F+L+NEN+K GP +ER++GL +P+ K VY++  +          G T  T 
Sbjct: 307 PKADLTVYLFALFNENKKLGPTSERNYGLFFPDEKKVYDIPFTTEGLKHYRDGGHTPVTG 366

Query: 368 ---KFKPLPEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIGKGGPCYE 427
                KP      ++   G  WCV         + G L YAC +G   C PI  G  CY 
Sbjct: 367 GDQVTKPPMSGGVSKSLNGYTWCVANGDAGEERLQGGLDYACGEGGADCRPIQPGANCYS 426

Query: 428 PDSLKRHASFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPS 461
           PD+L+ HASFAF+SY+ +  + GG+CYF G A    + P YG+C+FP+
Sbjct: 427 PDTLEAHASFAFNSYYQKKGRAGGSCYFGGAAYVVSQPPKYGRCEFPT 470

The following BLAST results are available for this feature:

BLAST of Chy4G075310.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q06915	4.4e-152	55.53	Probable glucan endo-1,3-beta-glucosidase A6 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q9FJU9	1.6e-90	39.49	Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590...	[more]
Q8VYE5	1.5e-88	36.13	Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360...	[more]
Q94G86	7.6e-88	37.72	Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1	[more]
Q9M069	4.2e-86	38.04	Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ...	[more]

BLAST of Chy4G075310.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LBR1	3.3e-267	97.85	(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G634360 PE=3...	[more]
A0A5A7VIC4	7.8e-261	95.69	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A1S3CCE3	7.8e-261	95.69	(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103499229 PE=3 SV...	[more]
A0A6J1EXC8	2.7e-237	86.85	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111437372 P...	[more]
A0A6J1K875	5.6e-235	85.99	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491531 PE=...	[more]

BLAST of Chy4G075310.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004144397.1	0.0	97.85	probable glucan endo-1,3-beta-glucosidase A6 [Cucumis sativus] >KGN58384.1 hypot...	[more]
XP_008460404.1	0.0	95.69	PREDICTED: probable glucan endo-1,3-beta-glucosidase A6 [Cucumis melo] >KAA00680...	[more]
XP_038886980.1	0.0	91.59	probable glucan endo-1,3-beta-glucosidase A6 [Benincasa hispida]	[more]
XP_022931078.1	5.88e-300	86.85	probable glucan endo-1,3-beta-glucosidase A6 [Cucurbita moschata]	[more]
XP_023532592.1	1.69e-299	86.85	probable glucan endo-1,3-beta-glucosidase A6 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Chy4G075310.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G07320.1	7.9e-189	68.34	O-Glycosyl hydrolases family 17 protein	[more]
AT3G23770.1	1.2e-157	56.21	O-Glycosyl hydrolases family 17 protein	[more]
AT4G14080.1	3.1e-153	55.53	O-Glycosyl hydrolases family 17 protein	[more]
AT4G26830.1	4.7e-101	40.60	O-Glycosyl hydrolases family 17 protein	[more]
AT2G05790.1	3.1e-100	39.74	O-Glycosyl hydrolases family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 373..458 e-value: 2.0E-40 score: 150.3
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 374..445 e-value: 4.1E-18 score: 65.8
None	No IPR available	GENE3D	1.20.58.1040		coord: 369..463 e-value: 1.9E-6 score: 30.1
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 25..355 e-value: 2.4E-119 score: 400.0
None	No IPR available	PANTHER	PTHR32227:SF341	BNAC03G35190D PROTEIN	coord: 12..456
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 26..347 e-value: 3.5E-103 score: 345.5
IPR000490	Glycoside hydrolase family 17	PROSITE	PS00587	GLYCOSYL_HYDROL_F17	coord: 259..272
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 12..456
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 26..347

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Chy4G075310	Chy4G075310	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Chy4G075310.1.exon1	Chy4G075310.1.exon1	exon
Chy4G075310.1.exon2	Chy4G075310.1.exon2	exon
Chy4G075310.1.exon3	Chy4G075310.1.exon3	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Chy4G075310.1.cds1	Chy4G075310.1.cds1	CDS
Chy4G075310.1.cds2	Chy4G075310.1.cds2	CDS
Chy4G075310.1.cds3	Chy4G075310.1.cds3	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Chy4G075310.1	Chy4G075310.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0046658	anchored component of plasma membrane
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity