Chy12G213140.1 (mRNA) Cucumber (hystrix) v1

Overview


Name	Chy12G213140.1
Type	mRNA
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	EH domain-binding protein 1-like protein 1
Location	chrH12: 10690869 .. 10693396 (+)
Sequence length	378
RNA-Seq Expression	Chy12G213140.1
Synteny	Chy12G213140.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTCTATCATCAACCAATATTGAACTGACTGAAGCACGGCGTTATCTCCCACAATTATTGCCTTCGCTTCCAATCTTCCATGACCTTTCACCCATTATCCCGAAAATTCCTTCATTGCCTTCGTTTCCGATCGTCCCTAACCTTTCACCCATTATCCCGGAAGTTCCTTCATTGCCTTCGCTTCCAATCGTCCCTAATCTTCCACCCATTATCCCGAAAGTTCCTTCATTGCCTTCGATTCCATTCATCCCTAAGATTGCTCCACCTACTAGTATCCCGCCGATCGTTCCTTCTATTCCATCCACCGATTTGCCTCCCCTTCCTAATATTCCAGTCCCCTAATATCCCGAGCATTGATTCCACTCCCTAGTACGCACCAAATACTATTATCCATTTCCTCCTTATATTTCCCTTAAATCCAATTTGTCTCCCCTCAGTATTCCACCTTTGACAATATACTTGTGTGCGTTATCAAAGTAGTGGAGCTTCTCTAGACTGGAACTTTAAAGCATGAGGTTTCCTGGTGGAATTTTAATTGCTTCAAGTTCTTTCTAATCAGTCATGTAAGCTACTATTATCATGTAACTTTAAAAAGTCATATATTTAAATAAAAAATTACTATGAGTGAAAATTATCAGTGTTATTTTGATAACTACCAGACAAATAAAGTTAAATCGAAGGAGAACATCACACATTAATAAAACGGCTTTGGTTATACGAACCAAATCCAAAAATTTGTTTTAAATTAATCAATAAAAACAAATGCGAGAACCAAGGATTGTTTTAAATAATTAAGTTCAATCATTTTGTTGTTGTTTTTTACAATTTAACTTCTTATAGACATTACTTCCATTACTAGCTTTCTTCCTTTCTTCTTTTGTTAGTTACTTTACCAAAACTGTAAAATAAAAATTTCTTTCAACCATAAATATATAGAAAATTATCCTAAAAATAAGAAGAAATAGGACTAACCTTCAAAATTGTTACCAAACAAAACTTTAAAACTATGAAATTACTAGAGACATCAACACGAGTACTTGTGAATCTGTCAATGACCAAGTTTCAAGAATCAAAAGCCTAATCTCACAAAACTATCTAAATTGGAGGAGGGGATCTATTCCAGCCGATTACAATTTGATTCTACGAATTCAAATATGAATTATTTGATAGGTTACCATACAACCCAACTTAGCTGAACCAACTGGTATTGAAAGCCAATCTGTTGAAAAAGTCTAACAAGCTTCCGAACTATTAATTAAACTAACATTATAATTAAACTAACATTATAATTAAACTAAATGTCAATCAACTAGGCACGATAGTGTTTCATGTAAATCTAAATATTTTTATTACTCTGATCAAGTGATACTACGTTCTATAATAATTAGATATGAAGATTGATATAGAGCTGGTTTGATAATTTTTGGTTTTTCTAATTAAGCCTAAAAACAACCATTCAACCTCTAAATTCTTTGTTTATTATCTACGTTTTTACCTTGTTTCATGTTAGCTCTAAAAAGTAGCTATTTACTACGAGAATTGGGAATTTGAGAAAATATTCAAGTATTGAGACAAAAAGAAGAACTACGAGATCAGAAGATAATGACGGCAATATTTAGATTTCTGTTTTCTAGTATATTTCATTCGTACATCGTAAACATTTTAGGTTTTTATATAACATGAAACCCTAGAATGAAGAGGGGCAGAAAAGACAATTTACATGCACATGGCAGAGTTTAATTATTGAATTTTATATGAAAATTAACATGCTCGTAACACCTTTTTTTTTTCATATTGCGAATATGGCAAAATTAATGATATCAAACGGCTATAAGACAATAATCATAGGGCTATCGGAGGGCTATCACGATTATTAGCTTTTAAATTTACTATTTTTGCAATTTAGAAAATATAGTGACATGAACTTTATTATCATACATTTTTTTTTGCTATTTTTCCAAGTTCTCCAATTTTATATAACATACATAATATTCCAAGGCATCCAATCACTATCTGCTTGTCGTGTCCACTATCCCATTCCGGTGCATTTTCTAACTCCTTCGCGTCGTCGTCAAGAAGATTTTCGACTTGAAAAACACAAAGTGAGTGAGACAAGGACTTAATTAGTGTGGCAGTCAACCACTTCATCCACATTAGAACGTAACGAATTTTCAGAGCACCCAGAGAATTTGATCATGTGATATGACTTGAAATGTATGTATTTTGTCTTTTAATTTAGAATGATTTTTTTAATTTAGTTAGGTTTAATGTCATTTGTTAGTGATTTCAAAATTATGTTTGAGATGTCGATTCAAGTGGAAGAGGAGAAAAAGTCCAAGGAGTGCCAAAAACAGGGTAGCGCTGCGATGCTTGCGCAGCAATAACCTTGACGCTTCTTCATGAAATTGAATGTTCCAATAGGTTGGGAAAGAGCACCGCAGTGCTCAGTAAAAAGTGCTGCTATGCTATGTGTAACACAACGGTGTGAAGCAACAAAGGGTCATAAGCGCTATAGCGCTGACTAG

mRNA sequence

ATGGGTCTATCATCAACCAATATTGAACTGACTGAAGCACGGCGTTATCTCCCACAATTATTGCCTTCGCTTCCAATCTTCCATGACCTTTCACCCATTATCCCGAAAATTCCTTCATTGCCTTCGTTTCCGATCGTCCCTAACCTTTCACCCATTATCCCGGAAGTTCCTTCATTGCCTTCGCTTCCAATCGTCCCTAATCTTCCACCCATTATCCCGAAAGTTCCTTCATTGCCTTCGATTCCATTCATCCCTAAGATTGCTCCACCTACTAGTTGGGAAAGAGCACCGCAGTGCTCAGTAAAAAGTGCTGCTATGCTATGTGTAACACAACGGTGTGAAGCAACAAAGGGTCATAAGCGCTATAGCGCTGACTAG

Coding sequence (CDS)

ATGGGTCTATCATCAACCAATATTGAACTGACTGAAGCACGGCGTTATCTCCCACAATTATTGCCTTCGCTTCCAATCTTCCATGACCTTTCACCCATTATCCCGAAAATTCCTTCATTGCCTTCGTTTCCGATCGTCCCTAACCTTTCACCCATTATCCCGGAAGTTCCTTCATTGCCTTCGCTTCCAATCGTCCCTAATCTTCCACCCATTATCCCGAAAGTTCCTTCATTGCCTTCGATTCCATTCATCCCTAAGATTGCTCCACCTACTAGTTGGGAAAGAGCACCGCAGTGCTCAGTAAAAAGTGCTGCTATGCTATGTGTAACACAACGGTGTGAAGCAACAAAGGGTCATAAGCGCTATAGCGCTGACTAG

Protein sequence

MGLSSTNIELTEARRYLPQLLPSLPIFHDLSPIIPKIPSLPSFPIVPNLSPIIPEVPSLPSLPIVPNLPPIIPKVPSLPSIPFIPKIAPPTSWERAPQCSVKSAAMLCVTQRCEATKGHKRYSAD*

Homology

[Close]

BLAST of Chy12G213140.1 vs. ExPASy TrEMBL
Match: A0A5D3CBA5 (Formin-2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2404G00010 PE=4 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 1.4e-10
Identity = 53/102 (51.96%), Postives = 67/102 (65.69%), Query Frame = 0


Query: 3   LSSTNIELTEARRYLPQLLPSL-----PIFHDLSPI-IPKIPSLPSFPIVPNLSPI---- 62
           LSST I+ +EA R+L Q LPS+     P+  ++ P+ IP +P LPS P VPN  P+    
Sbjct: 17  LSSTTIKGSEAGRHLQQSLPSVPSISSPLLPNIPPVTIPNVPLLPSIPFVPNTPPVTIPN 76

Query: 63  --IPEVPSLPSLPIVPNLPPI-IPKVPSLPSIPFIPKIAPPT 92
             IP VPSL S+P+VPN+P + IP VPSLPSIP IP I P T
Sbjct: 77  IPIPNVPSLLSIPLVPNIPSVTIPNVPSLPSIPLIPNIPPVT 118

[Close]

BLAST of Chy12G213140.1 vs. ExPASy TrEMBL
Match: A0A1S4E3J0 (formin-2-like OS=Cucumis melo OX=3656 GN=LOC107991890 PE=4 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 1.4e-10
Identity = 53/102 (51.96%), Postives = 67/102 (65.69%), Query Frame = 0


Query: 3   LSSTNIELTEARRYLPQLLPSL-----PIFHDLSPI-IPKIPSLPSFPIVPNLSPI---- 62
           LSST I+ +EA R+L Q LPS+     P+  ++ P+ IP +P LPS P VPN  P+    
Sbjct: 17  LSSTTIKGSEAGRHLQQSLPSVPSISSPLLPNIPPVTIPNVPLLPSIPFVPNTPPVTIPN 76

Query: 63  --IPEVPSLPSLPIVPNLPPI-IPKVPSLPSIPFIPKIAPPT 92
             IP VPSL S+P+VPN+P + IP VPSLPSIP IP I P T
Sbjct: 77  IPIPNVPSLLSIPLVPNIPSVTIPNVPSLPSIPLIPNIPPVT 118

[Close]

BLAST of Chy12G213140.1 vs. ExPASy TrEMBL
Match: A0A180H1P6 (Uncharacterized protein OS=Puccinia triticina (isolate 1-1 / race 1 (BBBD)) OX=630390 GN=PTTG_02477 PE=4 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 1.9e-07
Identity = 46/124 (37.10%), Postives = 71/124 (57.26%), Query Frame = 0


Query: 4   SSTNIELTEARRYLPQ-------------LLPSLPIFHDLSPIIPKIPSLPSFPIVPNLS 63
           SS   + + A+R++P               +PS+P    + P IP IPS+PS P +P+ +
Sbjct: 249 SSAAAKASRAKRHVPDDNTTVISALPSITAIPSIPSIPAI-PAIPAIPSIPSIPSIPSTT 308

Query: 64  --PIIPEVPSLPSLPIVPNLP-----PIIPKVPSLPSIPFIPKIAPPTSWERAPQCSVKS 108
             P IP +P++PS+P +P++P     P IP +P++PSIP IP I  P+S    PQ ++ S
Sbjct: 309 SIPAIPSIPAIPSIPAIPSIPAIPSIPAIPSIPAIPSIPSIPAIPLPSSAGSTPQTNLTS 368

[Close]

BLAST of Chy12G213140.1 vs. ExPASy TrEMBL
Match: A0A178WGX0 (Prolamin_like domain-containing protein OS=Arabidopsis thaliana OX=3702 GN=AXX17_At1g40250 PE=4 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 2.7e-06
Identity = 40/113 (35.40%), Postives = 65/113 (57.52%), Query Frame = 0


Query: 1   MGLSSTNIELTEARRYLPQLLPSLPIFHDLSPIIPKIPSLPSFPIVPNLSPIIPEVPSLP 60
           +G  ++++   +A    P LLP  P      P +P IP +P  P +PN+ P +P +P +P
Sbjct: 56  IGWGTSSLSDAKAPLSAPVLLPGFPSI----PWLPNIPGIPIIPSLPNIVPSLPNIPEIP 115

Query: 61  SLPIVPNLP--PIIPKVPSLPSIPFIPKIAPPTSWERAPQCSVKSAAMLCVTQ 112
           ++P +PN+P  P IP +P+LPSIP IP + PP  W ++   S  +    C+T+
Sbjct: 116 NIPRIPNIPGLPNIPGLPNLPSIPRIPGL-PPLPWVKSEVVSKSAKVENCLTK 163

[Close]

BLAST of Chy12G213140.1 vs. ExPASy TrEMBL
Match: A0A3P9APD1 (EH domain-binding protein 1-like protein 1 OS=Esox lucius OX=8010 PE=4 SV=2)

HSP 1 Score: 59.7 bits (143), Expect = 1.0e-05
Identity = 46/100 (46.00%), Postives = 60/100 (60.00%), Query Frame = 0


Query: 21  LPSLPIFHDLSPIIPKIPSLPSFPIVPNL-----SPIIPEVPSLPSLPIVPNLP--PIIP 80
           +PSLP    L P +P +PS+PS P VP+L     +P +P +PS+PSLP VP+LP  P +P
Sbjct: 302 VPSLPSVPSL-PSVPSLPSVPSAPSVPSLPSVPSAPSVPSLPSVPSLPSVPSLPSVPSLP 361

Query: 81  KVPSLPSIPFIPKIAPPTSWERAPQCSVKSAAMLCVTQRC 114
            VPS PS+P  P +   +S   AP  SV SA    V  RC
Sbjct: 362 SVPSAPSVPSAPSVPSASSVPSAP--SVPSAPSATVPLRC 398

[Close]

BLAST of Chy12G213140.1 vs. NCBI nr
Match: KAE8652802.1 (hypothetical protein Csa_022842 [Cucumis sativus])

HSP 1 Score: 119 bits (297), Expect = 2.93e-29
Identity = 73/102 (71.57%), Postives = 77/102 (75.49%), Query Frame = 0


Query: 1   MGLSSTNIELTEARRYLPQL-------------LPSLPIFHDLSPIIPKIPSLPSFPIVP 60
           MGLSSTNIELTEARRYLPQL             LPS PI  DLSPIIPKIPSLP   I P
Sbjct: 15  MGLSSTNIELTEARRYLPQLFPSIPSVISGVPSLPSFPIIPDLSPIIPKIPSLP---IAP 74

Query: 61  NLSPIIPEVPSLPSLPIVPNLPPIIPKVPSLPSIPFIPKIAP 89
           NL PIIP VPSLPSLPIVPNLPPIIP+VPSLPS+P +P + P
Sbjct: 75  NLPPIIPGVPSLPSLPIVPNLPPIIPRVPSLPSLPIVPNLPP 113

[Close]

BLAST of Chy12G213140.1 vs. NCBI nr
Match: XP_016902796.1 (PREDICTED: formin-2-like [Cucumis melo] >KAA0038820.1 formin-2-like [Cucumis melo var. makuwa] >TYK08468.1 formin-2-like [Cucumis melo var. makuwa])

HSP 1 Score: 72.8 bits (177), Expect = 6.83e-13
Identity = 53/102 (51.96%), Postives = 67/102 (65.69%), Query Frame = 0


Query: 3   LSSTNIELTEARRYLPQLLPSLP-----IFHDLSPI-IPKIPSLPSFPIVPNLSPI---- 62
           LSST I+ +EA R+L Q LPS+P     +  ++ P+ IP +P LPS P VPN  P+    
Sbjct: 17  LSSTTIKGSEAGRHLQQSLPSVPSISSPLLPNIPPVTIPNVPLLPSIPFVPNTPPVTIPN 76

Query: 63  --IPEVPSLPSLPIVPNLPPI-IPKVPSLPSIPFIPKIAPPT 91
             IP VPSL S+P+VPN+P + IP VPSLPSIP IP I P T
Sbjct: 77  IPIPNVPSLLSIPLVPNIPSVTIPNVPSLPSIPLIPNIPPVT 118

[Close]

BLAST of Chy12G213140.1 vs. NCBI nr
Match: OAV98957.1 (hypothetical protein PTTG_02477 [Puccinia triticina 1-1 BBBD Race 1])

HSP 1 Score: 62.8 bits (151), Expect = 1.87e-08
Identity = 41/94 (43.62%), Postives = 61/94 (64.89%), Query Frame = 0


Query: 21  LPSLPIFHDLSPIIPKIPSLPSFPIVPNLS--PIIPEVPSLPSLPIVPNLP-----PIIP 80
           +PS+P    + P IP IPS+PS P +P+ +  P IP +P++PS+P +P++P     P IP
Sbjct: 279 IPSIPSIPAI-PAIPAIPSIPSIPSIPSTTSIPAIPSIPAIPSIPAIPSIPAIPSIPAIP 338

Query: 81  KVPSLPSIPFIPKIAPPTSWERAPQCSVKSAAML 107
            +P++PSIP IP I  P+S    PQ ++ SAA L
Sbjct: 339 SIPAIPSIPSIPAIPLPSSAGSTPQTNLTSAAQL 371

[Close]

BLAST of Chy12G213140.1 vs. NCBI nr
Match: KAG7648731.1 (Prolamin-like domain [Arabidopsis thaliana x Arabidopsis arenosa] >OAP17496.1 hypothetical protein AXX17_AT1G40250 [Arabidopsis thaliana])

HSP 1 Score: 59.3 bits (142), Expect = 1.45e-07
Identity = 40/113 (35.40%), Postives = 65/113 (57.52%), Query Frame = 0


Query: 1   MGLSSTNIELTEARRYLPQLLPSLPIFHDLSPIIPKIPSLPSFPIVPNLSPIIPEVPSLP 60
           +G  ++++   +A    P LLP  P      P +P IP +P  P +PN+ P +P +P +P
Sbjct: 56  IGWGTSSLSDAKAPLSAPVLLPGFPSI----PWLPNIPGIPIIPSLPNIVPSLPNIPEIP 115

Query: 61  SLPIVPNLP--PIIPKVPSLPSIPFIPKIAPPTSWERAPQCSVKSAAMLCVTQ 111
           ++P +PN+P  P IP +P+LPSIP IP + PP  W ++   S  +    C+T+
Sbjct: 116 NIPRIPNIPGLPNIPGLPNLPSIPRIPGL-PPLPWVKSEVVSKSAKVENCLTK 163

[Close]

BLAST of Chy12G213140.1 vs. NCBI nr
Match: XP_028976810.2 (titin isoform X10 [Esox lucius])

HSP 1 Score: 58.5 bits (140), Expect = 6.03e-07
Identity = 46/99 (46.46%), Postives = 59/99 (59.60%), Query Frame = 0


Query: 22  PSLPIFHDLSPIIPKIPSLPSFPIVPNL-----SPIIPEVPSLPSLPIVPNLP--PIIPK 81
           PSLP    L P +P +PS+PS P VP+L     +P +P +PS+PSLP VP+LP  P +P 
Sbjct: 309 PSLPSVPSL-PSVPSLPSVPSAPSVPSLPSVPSAPSVPSLPSVPSLPSVPSLPSVPSLPS 368

Query: 82  VPSLPSIPFIPKIAPPTSWERAPQCSVKSAAMLCVTQRC 113
           VPS PS+P  P +   +S   AP  SV SA    V  RC
Sbjct: 369 VPSAPSVPSAPSVPSASSVPSAP--SVPSAPSATVPLRC 404

[Close]

BLAST of Chy12G213140.1 vs. TAIR 10
Match: AT1G44224.1 (ECA1 gametogenesis related family protein )

HSP 1 Score: 58.2 bits (139), Expect = 5.7e-09
Identity = 44/111 (39.64%), Postives = 67/111 (60.36%), Query Frame = 0


Query: 1   MGLSSTNIELTEARRYLPQLLPSLPIFHDLSPIIPKIPSLPSFPIVPNLSPIIPEVPSLP 60
           +G  ++++  ++A    P LLP  P    L P IP IP +PS P +P+L P IPE+P++P
Sbjct: 56  IGWGTSSLSDSKAPLSPPVLLPGFPSIPWL-PNIPGIPIIPSLPNIPSL-PTIPEIPNIP 115

Query: 61  SLPIVPNLPPIIPKVPSLPSIPFIPKIAPPTSWERAPQCSVKSAAMLCVTQ 112
            +P +P LP  IP +P+LPSIP IP + PP  W ++   S  +    C+T+
Sbjct: 116 RIPNIPGLPN-IPGLPNLPSIPRIPGL-PPLPWVKSEVVSKSAKVENCLTK 162

[Close]

BLAST of Chy12G213140.1 vs. TAIR 10
Match: AT5G15780.1 (Pollen Ole e 1 allergen and extensin family protein )

HSP 1 Score: 43.5 bits (101), Expect = 1.5e-04
Identity = 32/83 (38.55%), Postives = 48/83 (57.83%), Query Frame = 0


Query: 18  PQLLPSLPIFHDL-------SPIIPKIPSLPSFPIVPNLSPIIPEVPSLPSLPIVPNLPP 77
           P ++P  P+   +       +P+IP  PSLP  P++P   P +P +P LP+ P  P LPP
Sbjct: 269 PSIIPPNPLIPSIPTPTLPPNPLIPSPPSLPPIPLIPT-PPTLPTIPLLPT-PPTPTLPP 328

Query: 78  I--IPKVPSLPSIPFIPKIAPPT 92
           I  IP +P LP +P +P + PP+
Sbjct: 329 IPTIPTLPPLPVLPPVPIVNPPS 349

The following BLAST results are available for this feature:

BLAST of Chy12G213140.1 vs.


Match Name	E-value	Identity	Description

BLAST of Chy12G213140.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CBA5	1.4e-10	51.96	Formin-2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2404G00010...	[more]
A0A1S4E3J0	1.4e-10	51.96	formin-2-like OS=Cucumis melo OX=3656 GN=LOC107991890 PE=4 SV=1	[more]
A0A180H1P6	1.9e-07	37.10	Uncharacterized protein OS=Puccinia triticina (isolate 1-1 / race 1 (BBBD)) OX=6...	[more]
A0A178WGX0	2.7e-06	35.40	Prolamin_like domain-containing protein OS=Arabidopsis thaliana OX=3702 GN=AXX17...	[more]
A0A3P9APD1	1.0e-05	46.00	EH domain-binding protein 1-like protein 1 OS=Esox lucius OX=8010 PE=4 SV=2	[more]

BLAST of Chy12G213140.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8652802.1	2.93e-29	71.57	hypothetical protein Csa_022842 [Cucumis sativus]	[more]
XP_016902796.1	6.83e-13	51.96	PREDICTED: formin-2-like [Cucumis melo] >KAA0038820.1 formin-2-like [Cucumis mel...	[more]
OAV98957.1	1.87e-08	43.62	hypothetical protein PTTG_02477 [Puccinia triticina 1-1 BBBD Race 1]	[more]
KAG7648731.1	1.45e-07	35.40	Prolamin-like domain [Arabidopsis thaliana x Arabidopsis arenosa] >OAP17496.1 hy...	[more]
XP_028976810.2	6.03e-07	46.46	titin isoform X10 [Esox lucius]	[more]

BLAST of Chy12G213140.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G44224.1	5.7e-09	39.64	ECA1 gametogenesis related family protein	[more]
AT5G15780.1	1.5e-04	38.55	Pollen Ole e 1 allergen and extensin family protein	[more]

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Chy12G213140	Chy12G213140	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Chy12G213140.1.exon1	Chy12G213140.1.exon1	exon
Chy12G213140.1.exon2	Chy12G213140.1.exon2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Chy12G213140.1.cds1	Chy12G213140.1.cds1	CDS
Chy12G213140.1.cds2	Chy12G213140.1.cds2	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Chy12G213140.1	Chy12G213140.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006810	transport
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016020	membrane