Chy12G210330.1 (mRNA) Cucumber (hystrix) v1

BLAST of Chy12G210330.1 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 2.1e-142
Identity = 262/535 (48.97%), Postives = 350/535 (65.42%), Query Frame = 0

Query: 11  FISFTIVSLFSTQWSTASSNKNKDFLQCLSIH-----STDIPIYTPINSSYSSILRSYAQ 70
           F+   I    S     + +N  ++FL+C S H     +    +YT  +  Y SIL S  Q
Sbjct: 10  FVCKIIFFFLSFHIQISIANPRENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQ 69

Query: 71  NPRFSTQYKTLKPLVIIKPSRVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYISDLPFV 130
           N RF +   T KPLVI+ PS  SH+Q+T++CSK  GLQIR RSGGHD EG+SYIS +PFV
Sbjct: 70  NLRFISD-TTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFV 129

Query: 131 VVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGGHFSGG 190
           VVDL N+ SI++D  + TAWV++GAT+GE+YY I EK+  L+FP G CPTVGVGGHFSGG
Sbjct: 130 VVDLRNMHSIKIDVHSQTAWVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGG 189

Query: 191 GYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVGWKVKL 250
           GYG LMR YGLAADN+IDA+LV+ +G V DR++MGE LFWAIRGGGG +FGI+  WK+KL
Sbjct: 190 GYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKL 249

Query: 251 VRVPPIVTTCRLDKSLD-KNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKRNPT- 310
           V VP   T   + K+++     K+  +WQ +A K +++L++  +    N T    +N T 
Sbjct: 250 VAVPSKSTIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTT 309

Query: 311 --ASFFSLYLGKTDKLVTIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPL----E 370
               F S++ G  D LV +MN +FP LG+ K +CKE SWI +T+  +G  N        E
Sbjct: 310 VHGYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKE 369

Query: 371 ILLTKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNIS 430
           ILL + +     ++ IK DYVK+PI + A   I ++L  ++V      L+PYGG M  IS
Sbjct: 370 ILLDR-SAGKKTAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEIS 429

Query: 431 SSKTPFSHRAEFLYKISYTVGWAEKGTGANERHLNWIREFYSFMTPFVSNSPRAAYVNYR 490
            S  PF HRA  +Y++ YT  W ++    NE+H+NW+R  Y+F TP+VS +PR AY+NYR
Sbjct: 430 ESAIPFPHRAGIMYELWYTASWEKQED--NEKHINWVRSVYNFTTPYVSQNPRLAYLNYR 489

Query: 491 DLDIGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPL 533
           DLD+G  N     +Y +A IWG KYFG NF RLV+VKT VDP+NFFR+EQSIPPL
Sbjct: 490 DLDLGKTNHASPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 540

BLAST of Chy12G210330.1 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 4.7e-142
Identity = 264/535 (49.35%), Postives = 356/535 (66.54%), Query Frame = 0

Query: 11  FISFTIVSLFSTQWSTASSNKNKDFLQCLSIH----STDIP-IYTPINSSYSSILRSYAQ 70
           F+   I   FS    T+ +N  ++FL+C S +    +T++  +YT  N  Y S+L S   
Sbjct: 10  FVCKIIFFFFSFNIQTSIANPRENFLKCFSQYIPNNATNLKLVYTQNNPLYMSVLNSTIH 69

Query: 71  NPRFSTQYKTLKPLVIIKPSRVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYISDLPFV 130
           N RF T   T KPLVI+ PS VSH+Q T++CSK  GLQIR RSGGHD+EG+SYIS +PFV
Sbjct: 70  NLRF-TSDTTPKPLVIVTPSHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFV 129

Query: 131 VVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGGHFSGG 190
           +VDL N++SI++D  + TAWV++GAT+GE+YY + EK+  L+  AG CPTV  GGHF GG
Sbjct: 130 IVDLRNMRSIKIDVHSQTAWVEAGATLGEVYYWVNEKNENLSLAAGYCPTVCAGGHFGGG 189

Query: 191 GYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVGWKVKL 250
           GYG LMR YGLAADN+IDA+LV+ +G V DR++MGE LFWA+RGGG  SFGI+V WK++L
Sbjct: 190 GYGPLMRNYGLAADNIIDAHLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKIRL 249

Query: 251 VRVPPIVTTCRLDKSLD-KNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKRNPTA 310
           V VP   T   + K ++     K+V +WQ +A K +++LL+  +    N T    +N TA
Sbjct: 250 VAVPK-STMFSVKKIMEIHELVKLVNKWQNIAYKYDKDLLLMTHFITRNITDNQGKNKTA 309

Query: 311 ---SFFSLYLGKTDKLVTIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQP----LE 370
               F S++LG  D LV +MN +FP LG+ K +C++ SWI + +  +G  N        E
Sbjct: 310 IHTYFSSVFLGGVDSLVDLMNKSFPELGIKKTDCRQLSWIDTIIFYSGVVNYDTDNFNKE 369

Query: 371 ILLTKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNIS 430
           ILL + +   N ++KIK DYVK+PI +  F  I ++L  +++      L+PYGG M  IS
Sbjct: 370 ILLDR-SAGQNGAFKIKLDYVKKPIPESVFVQILEKLYEEDIGAGMYALYPYGGIMDEIS 429

Query: 431 SSKTPFSHRAEFLYKISYTVGWAEKGTGANERHLNWIREFYSFMTPFVSNSPRAAYVNYR 490
            S  PF HRA  LY++ Y   W ++    NE+HLNWIR  Y+FMTP+VS +PR AY+NYR
Sbjct: 430 ESAIPFPHRAGILYELWYICSWEKQED--NEKHLNWIRNIYNFMTPYVSKNPRLAYLNYR 489

Query: 491 DLDIGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPL 533
           DLDIG N+     +Y +A IWG KYFG NF RLV+VKT VDP+NFFR+EQSIPPL
Sbjct: 490 DLDIGINDPKNPNNYTQARIWGEKYFGKNFDRLVKVKTLVDPNNFFRNEQSIPPL 539

BLAST of Chy12G210330.1 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 502.7 bits (1293), Expect = 5.2e-141
Identity = 259/535 (48.41%), Postives = 348/535 (65.05%), Query Frame = 0

Query: 11  FISFTIVSLFSTQWSTASSNKNKDFLQCLSIHSTDIP-----IYTPINSSYSSILRSYAQ 70
           F+   I    S     + +N  ++FL+C S +  + P     IYT  +  Y S+L S  Q
Sbjct: 10  FVCKIIFFFLSFNIQISIANPQENFLKCFSEYIPNNPANPKFIYTQHDQLYMSVLNSTIQ 69

Query: 71  NPRFSTQYKTLKPLVIIKPSRVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYISDLPFV 130
           N RF T   T KPLVI+ PS VSH+Q++++CSK  GLQIR RSGGHD EGLSYIS +PF 
Sbjct: 70  NLRF-TSDTTPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFA 129

Query: 131 VVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGGHFSGG 190
           +VDL N+ +++VD  + TAWV++GAT+GE+YY I E +   +FP G CPTVGVGGHFSGG
Sbjct: 130 IVDLRNMHTVKVDIHSQTAWVEAGATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGG 189

Query: 191 GYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVGWKVKL 250
           GYG LMR YGLAADN+IDA+LV+ +G V DR++MGE LFWAIRGGGG +FGI+  WK+KL
Sbjct: 190 GYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKL 249

Query: 251 VRVPPIVTTCRLDKSLD-KNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKRNPT- 310
           V VP   T   + K+++     K+  +WQ +A K +++L++  +    N T    +N T 
Sbjct: 250 VVVPSKATIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKTT 309

Query: 311 --ASFFSLYLGKTDKLVTIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPL----E 370
               F S++LG  D LV +MN +FP LG+ K +CKE SWI +T+  +G  N        E
Sbjct: 310 VHGYFSSIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFKKE 369

Query: 371 ILLTKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNIS 430
           ILL + +     ++ IK DYVK+ I + A   I ++L  +EV      L+PYGG M  IS
Sbjct: 370 ILLDR-SAGKKTAFSIKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEIS 429

Query: 431 SSKTPFSHRAEFLYKISYTVGWAEKGTGANERHLNWIREFYSFMTPFVSNSPRAAYVNYR 490
            S  PF HRA  +Y++ YT  W ++    NE+H+NW+R  Y+F TP+VS +PR AY+NYR
Sbjct: 430 ESAIPFPHRAGIMYELWYTATWEKQED--NEKHINWVRSVYNFTTPYVSQNPRLAYLNYR 489

Query: 491 DLDIGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPL 533
           DLD+G  N     +Y +A IWG KYFG NF RLV+VKT  DP+NFFR+EQSIPPL
Sbjct: 490 DLDLGKTNPESPNNYTQARIWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIPPL 540

BLAST of Chy12G210330.1 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 2.0e-140
Identity = 262/537 (48.79%), Postives = 349/537 (64.99%), Query Frame = 0

Query: 11  FISFTIVSLFSTQWSTASSNKNKDFLQCLSIH-STDIP----IYTPINSSYSSILRSYAQ 70
           F+SF I          + +N  ++FL+C S +  T++     +YT  +  Y SIL S  Q
Sbjct: 18  FLSFNI--------QISIANPQENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQ 77

Query: 71  NPRFSTQYKTLKPLVIIKPSRVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYISDLPFV 130
           N RF T   T KPLVII P  VSH+Q T++CSK  GLQIR RSGGHD EG+SYIS +PFV
Sbjct: 78  NLRF-TSDTTPKPLVIITPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFV 137

Query: 131 VVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGGHFSGG 190
           +VDL N+ S+++D  + TAWV++GAT+GE+YY I E +  L+FPAG CPTVG GGHFSGG
Sbjct: 138 IVDLRNMHSVKIDVHSQTAWVEAGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGG 197

Query: 191 GYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVGWKVKL 250
           GYG LMR YGLAADN+IDA+LV+ +G V DR++MGE LFWAIRGGGG +FGI+  WK++L
Sbjct: 198 GYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRL 257

Query: 251 VRVPPIVTTCRLDKSLD-KNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKRNPT- 310
           V VP + T   + K+++     K+V +WQ +A   E+ LL+  +    N T    +N T 
Sbjct: 258 VAVPSMSTIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTT 317

Query: 311 --ASFFSLYLGKTDKLVTIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPL----E 370
             + F S++ G  D LV +MN +FP LG+ K +CK+ SWI + +  +G  N        E
Sbjct: 318 IHSYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKE 377

Query: 371 ILLTKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNIS 430
           ILL + +     ++ IK DYVK+PI + A   I ++L  ++V       +PYGG M  IS
Sbjct: 378 ILLDR-SGGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEIS 437

Query: 431 SSKTPFSHRAEFLYKISYTVGWAEKGTGANERHLNWIREFYSFMTPFVSNSPRAAYVNYR 490
            S  PF HRA  +Y+I Y   W ++    NE+H+NWIR  Y+F TP+VS +PR AY+NYR
Sbjct: 438 ESAIPFPHRAGIMYEIWYIASWEKQED--NEKHINWIRNVYNFTTPYVSQNPRMAYLNYR 497

Query: 491 DLDIGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPLTL 535
           DLD+G  N     +Y +A IWG KYFG NF RLV+VKT VDP NFFR+EQSIPPL L
Sbjct: 498 DLDLGKTNFESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPLPL 542

BLAST of Chy12G210330.1 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 2.0e-140
Identity = 263/537 (48.98%), Postives = 348/537 (64.80%), Query Frame = 0

Query: 11  FISFTIVSLFSTQWSTASSNKNKDFLQCLSIH-STDIP----IYTPINSSYSSILRSYAQ 70
           F+SF I          + +N  ++FL+CLS +  T++     +YT  +  Y SIL S  Q
Sbjct: 18  FLSFNI--------QISIANPQENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQ 77

Query: 71  NPRFSTQYKTLKPLVIIKPSRVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYISDLPFV 130
           N RF T   T KPLVI  P  VSH+Q T++CSK  GLQIR RSGGHD EG+SYIS +PFV
Sbjct: 78  NLRF-TSDTTPKPLVITTPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFV 137

Query: 131 VVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGGHFSGG 190
           +VDL N+ S+++D  + TAWV+SGAT+GE+YY I E +  L+FPAG CPTVG GGHFSGG
Sbjct: 138 IVDLRNMHSVKIDVHSQTAWVESGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGG 197

Query: 191 GYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVGWKVKL 250
           GYG LMR YGLAADN+IDA+LV+ +G V DR++MGE LFWAIRGGGG +FGI+  WK++L
Sbjct: 198 GYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRL 257

Query: 251 VRVPPIVTTCRLDKSLD-KNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKRNPT- 310
           V VP + T   + K+++     K+V +WQ +A   E+ LL+  +    N T    +N T 
Sbjct: 258 VAVPSMSTIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTT 317

Query: 311 --ASFFSLYLGKTDKLVTIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPL----E 370
             + F S++ G  D LV +MN +FP LG+ K +CK+ SWI + +  +G  N        E
Sbjct: 318 IHSYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTNFKKE 377

Query: 371 ILLTKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNIS 430
           ILL + +     ++ IK DYVK+PI + A   I ++L  ++V       +PYGG M  IS
Sbjct: 378 ILLDR-SGGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEIS 437

Query: 431 SSKTPFSHRAEFLYKISYTVGWAEKGTGANERHLNWIREFYSFMTPFVSNSPRAAYVNYR 490
            S  PF HRA   Y+I Y   W ++    NE+H+NWIR  Y+F TP+VS +PR AY+NYR
Sbjct: 438 ESAIPFPHRAGITYEIWYIASWEKQED--NEKHINWIRNVYNFTTPYVSQNPRMAYLNYR 497

Query: 491 DLDIGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPLTL 535
           DLD+G  N     +Y +A IWG KYFG NF RLV+VKT VDP NFFR+EQSIPPL L
Sbjct: 498 DLDLGKTNFESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPLPL 542

BLAST of Chy12G210330.1 vs. ExPASy TrEMBL
Match: A0A5A7TYM8 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00390 PE=3 SV=1)

HSP 1 Score: 1037.7 bits (2682), Expect = 1.7e-299
Identity = 507/535 (94.77%), Postives = 519/535 (97.01%), Query Frame = 0

Query: 1   MKLFPLNPLLFISFTIVSLFSTQWSTASSNKNKDFLQCLSIHSTDIPIYTPINSSYSSIL 60
           MK FPLNPLLF SFT+VSL STQWSTASS KNKDFL+CLSIHSTDIPIYTPINSSY+S L
Sbjct: 1   MKSFPLNPLLFTSFTVVSLSSTQWSTASSYKNKDFLRCLSIHSTDIPIYTPINSSYTSTL 60

Query: 61  RSYAQNPRFSTQYKTLKPLVIIKPSRVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYIS 120
           RSYAQNPRFSTQYKTLKPL IIKPS VSH+QSTVICSKSHGLQIRIRSGGHDTEGLSYIS
Sbjct: 61  RSYAQNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEGLSYIS 120

Query: 121 DLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGG 180
           DLPF+VVDLINLKSIEVDTTNNT WVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGG
Sbjct: 121 DLPFIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGG 180

Query: 181 HFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVG 240
            FSGGGYGWLMRKYGLAADNVIDAYLVDANG VFD EAMGEGLFWAIRGGGGGSFGIVVG
Sbjct: 181 QFSGGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGGGGGSFGIVVG 240

Query: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKR 300
           WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKR
Sbjct: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKR 300

Query: 301 NPTASFFSLYLGKTDKLVTIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360
           NPTASFFSLYLGKTDKL+ IMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL
Sbjct: 301 NPTASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360

Query: 361 TKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNISSSK 420
           TKPTLSNNISYKIKSDYVKQPIS+HAFKGIWDRL+S EVETSQLFLFPYGGKM+NISSSK
Sbjct: 361 TKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPYGGKMNNISSSK 420

Query: 421 TPFSHRAEFLYKISYTVGWAEKGTGANERHLNWIREFYSFMTPFVSNSPRAAYVNYRDLD 480
           TPFSHRAEFLYKISYTVGWAE+GTGANERHLNWIRE YSFMTPFVSNSPRAAYVNYRDLD
Sbjct: 421 TPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSNSPRAAYVNYRDLD 480

Query: 481 IGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPLTLS 536
           IGTNNK GKTSYEEASIWG+KYFGNNFKRLVQVKT VDPSNFFRH+QSIPPLTLS
Sbjct: 481 IGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSIPPLTLS 535

BLAST of Chy12G210330.1 vs. ExPASy TrEMBL
Match: A0A1S3BDG4 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488681 PE=3 SV=1)

HSP 1 Score: 1037.7 bits (2682), Expect = 1.7e-299
Identity = 507/535 (94.77%), Postives = 519/535 (97.01%), Query Frame = 0

Query: 1   MKLFPLNPLLFISFTIVSLFSTQWSTASSNKNKDFLQCLSIHSTDIPIYTPINSSYSSIL 60
           MK FPLNPLLF SFT+VSL STQWSTASS KNKDFL+CLSIHSTDIPIYTPINSSY+S L
Sbjct: 1   MKSFPLNPLLFTSFTVVSLSSTQWSTASSYKNKDFLRCLSIHSTDIPIYTPINSSYTSTL 60

Query: 61  RSYAQNPRFSTQYKTLKPLVIIKPSRVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYIS 120
           RSYAQNPRFSTQYKTLKPL IIKPS VSH+QSTVICSKSHGLQIRIRSGGHDTEGLSYIS
Sbjct: 61  RSYAQNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEGLSYIS 120

Query: 121 DLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGG 180
           DLPF+VVDLINLKSIEVDTTNNT WVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGG
Sbjct: 121 DLPFIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGG 180

Query: 181 HFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVG 240
            FSGGGYGWLMRKYGLAADNVIDAYLVDANG VFD EAMGEGLFWAIRGGGGGSFGIVVG
Sbjct: 181 QFSGGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGGGGGSFGIVVG 240

Query: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKR 300
           WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKR
Sbjct: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKR 300

Query: 301 NPTASFFSLYLGKTDKLVTIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360
           NPTASFFSLYLGKTDKL+ IMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL
Sbjct: 301 NPTASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360

Query: 361 TKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNISSSK 420
           TKPTLSNNISYKIKSDYVKQPIS+HAFKGIWDRL+S EVETSQLFLFPYGGKM+NISSSK
Sbjct: 361 TKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPYGGKMNNISSSK 420

Query: 421 TPFSHRAEFLYKISYTVGWAEKGTGANERHLNWIREFYSFMTPFVSNSPRAAYVNYRDLD 480
           TPFSHRAEFLYKISYTVGWAE+GTGANERHLNWIRE YSFMTPFVSNSPRAAYVNYRDLD
Sbjct: 421 TPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSNSPRAAYVNYRDLD 480

Query: 481 IGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPLTLS 536
           IGTNNK GKTSYEEASIWG+KYFGNNFKRLVQVKT VDPSNFFRH+QSIPPLTLS
Sbjct: 481 IGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSIPPLTLS 535

BLAST of Chy12G210330.1 vs. ExPASy TrEMBL
Match: A0A6J1EGP1 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111434010 PE=3 SV=1)

HSP 1 Score: 785.4 bits (2027), Expect = 1.5e-223
Identity = 382/523 (73.04%), Postives = 440/523 (84.13%), Query Frame = 0

Query: 16  IVSLFSTQWSTASSNKNKDFLQCLSIHSTDIP--IYTPINSSYSSILRSYAQNPRFSTQY 75
           I+S F+  W+ A ++K++ FLQCLSIHS DI   IYTPINSSYSS+L SYA NPR S   
Sbjct: 11  ILSSFTVLWAAALAHKHEHFLQCLSIHSRDISKLIYTPINSSYSSLLTSYALNPRVSIP- 70

Query: 76  KTLKPLVIIKPSRVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYIS-DLPFVVVDLINL 135
            T  P VIIKPS +SH+Q+ V+CSKSHG QIR RSGGHD EGLSYIS  LPF++VDL NL
Sbjct: 71  ATPNPHVIIKPSHLSHIQAAVVCSKSHGFQIRTRSGGHDAEGLSYISHHLPFIIVDLKNL 130

Query: 136 KSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGGHFSGGGYGWLMR 195
            SI V+  NNTAWVQSGATIGE+YY IA+KSRTLAFPAGVCPTVG GGH SGGGYGWLMR
Sbjct: 131 NSITVNIENNTAWVQSGATIGEVYYEIAKKSRTLAFPAGVCPTVGFGGHLSGGGYGWLMR 190

Query: 196 KYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVGWKVKLVRVPPIV 255
           KYGLAADNV+DAYLVDA G V DREAMGE LFWAIRGGGGGSFGIVVGWKVKLV VP IV
Sbjct: 191 KYGLAADNVVDAYLVDAYGKVLDREAMGEDLFWAIRGGGGGSFGIVVGWKVKLVAVPAIV 250

Query: 256 TTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKRNPTASFFSLYLG 315
           TTC+L+K+LD   KK+VYQWQYVANKM+ENLL+G+NLTGGNPT+G K N  A+FFSL+LG
Sbjct: 251 TTCQLEKNLDGYAKKLVYQWQYVANKMDENLLLGVNLTGGNPTQGAKPNHKATFFSLFLG 310

Query: 316 KTDKLVTIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILLTKPTLSNNISYK 375
            +  L+  MNTTFP LGLT  +C++TSWIQSTL+AAGFT+  PLE LLTK T  N ++YK
Sbjct: 311 NSHDLIATMNTTFPQLGLTNNDCQQTSWIQSTLLAAGFTSRDPLEFLLTK-TSFNTVAYK 370

Query: 376 IKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNISSSKTPFSHRAEFLYK 435
           IKSDYV +PIS+HAFKGIWDRLKS ++E++QL LFPYGG+MS I + +TPFSHRAEF+YK
Sbjct: 371 IKSDYVNKPISKHAFKGIWDRLKSPKMESAQLLLFPYGGRMSKIPTWETPFSHRAEFMYK 430

Query: 436 ISYTVGWAEKGTGANERHLNWIREFYSFMTPFVSNSPRAAYVNYRDLDIGTNNKYGKTSY 495
           ISY VGWAE+   A+ER+LNW+REFY +M+PFVSNSPRAAYVNYRDLDIG NN+YG TSY
Sbjct: 431 ISYVVGWAEQSREASERNLNWVREFYEYMSPFVSNSPRAAYVNYRDLDIGMNNEYGSTSY 490

Query: 496 EEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPLTLS 536
           EEA +WG  YFG+NFKRLV+VK  VDP NFFRHEQS+PPL LS
Sbjct: 491 EEARVWGEMYFGDNFKRLVRVKNKVDPLNFFRHEQSVPPLALS 531

BLAST of Chy12G210330.1 vs. ExPASy TrEMBL
Match: A0A6J1DF27 (berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111020224 PE=3 SV=1)

HSP 1 Score: 705.3 bits (1819), Expect = 2.0e-199
Identity = 354/545 (64.95%), Postives = 430/545 (78.90%), Query Frame = 0

Query: 1   MKLFPLNPLLFISFTIVSLFSTQWSTASSNKNKDFLQCLSIHSTDIP-----IYTPINSS 60
           MK  PL  +L ++F ++S  S+ W+ AS+ K++ FLQCLS HS D       IYTPIN S
Sbjct: 1   MKYSPLT-ILSLAFILLS-SSSSWAAASAEKHEAFLQCLSHHSADSSSISKVIYTPINPS 60

Query: 61  YSSILRSYAQNPRFSTQYKTLKPLVIIKPSRVSHLQSTVICSKSHGLQIRIRSGGHDTEG 120
           YSS+L    +N RFS+  +T KPL+II PS VSH+Q+ VICSK+HGLQIRIRSGGHD EG
Sbjct: 61  YSSVLNFSVRNLRFSSA-ETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEG 120

Query: 121 LSYISDLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPT 180
           LSY++ LPF+++DLINLKSI V+  N+TAW+QSGAT+GELYY IA+KSRTLAFPAGVCPT
Sbjct: 121 LSYVAHLPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPT 180

Query: 181 VGVGGHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSF 240
           VGVGGHFSGGGYG ++RKYGLAADNVIDAYL+DANG  +DRE+MGE LFWAIRGGGGGSF
Sbjct: 181 VGVGGHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPT 300
           GIVV WKVKLV VP IVTTC  D++LD++T  ++++WQ VA K++ENL  GI L GGN +
Sbjct: 241 GIVVAWKVKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGGNAS 300

Query: 301 K--GVKR-NPTASFFSLYLGKTDKLVTIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTN 360
              GVK+ NPTA+FFSL+LGK+D+L+ I+NT FP L L K +C ETSWI+STL+AA  T 
Sbjct: 301 TEGGVKKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAA--TR 360

Query: 361 GQPLEIL-----LTKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLF 420
            Q +E L      T  T+ N    K+KSDYVK+PI +   +GIW+RLK+Q++E  QL   
Sbjct: 361 NQTIESLESLLNRTPLTIENT---KLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFV 420

Query: 421 PYGGKMSNISSSKTPFSHRAEFLYKISYTVGWAEKGTGANERHLNWIREFYSFMTPFVSN 480
           PYGG+MS IS S+TPFSHRA  LYKI Y VGW E+   A ERHL+WIRE Y +MTPFVS 
Sbjct: 421 PYGGRMSRISDSETPFSHRARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSK 480

Query: 481 SPRAAYVNYRDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQ 533
           SPRA YVNYRDLDIGTNNKYGKTSY++AS+WG KYFGNNF RLV +K+ VDPSNFF++EQ
Sbjct: 481 SPRATYVNYRDLDIGTNNKYGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQ 537

BLAST of Chy12G210330.1 vs. ExPASy TrEMBL
Match: A0A6J1DEU9 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020168 PE=3 SV=1)

HSP 1 Score: 697.6 bits (1799), Expect = 4.1e-197
Identity = 341/537 (63.50%), Postives = 426/537 (79.33%), Query Frame = 0

Query: 1   MKLFPLNPLLFISFTIVSLFSTQWSTASSNKNKDFLQCLSIHSTDIP-----IYTPINSS 60
           MK FPLNPL F     + L S+ +  AS++K++ FLQCL+ HS         IYTP N S
Sbjct: 1   MKSFPLNPLAFAFACSIVLSSSLFWAASAHKHEGFLQCLTRHSPSNNSISKFIYTPANFS 60

Query: 61  YSSILRSYAQNPRFSTQYKTLKPLVIIKPSRVSHLQSTVICSKSHGLQIRIRSGGHDTEG 120
           YSSIL    QN RFST  +T KPL++I PS +SH+Q+ VICSK+HGLQIR RSGGHD EG
Sbjct: 61  YSSILNFSIQNLRFSTA-ETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGGHDFEG 120

Query: 121 LSYISDLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPT 180
           LSY+S LPF+V+DLI+L+SI +D  N+TAWVQSGAT+GELYYRIA+KS+TLAFPAG CPT
Sbjct: 121 LSYVSHLPFIVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPAGSCPT 180

Query: 181 VGVGGHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSF 240
           VGVGGH SGGGYG+L+RKYGLAAD+VIDA+LVDANG + +RE+M + LFW IRGGGGGSF
Sbjct: 181 VGVGGHLSGGGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGGGGGSF 240

Query: 241 GIVVGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGN-- 300
           GIVV WK++LV VP  +TTC ++++LD++  K++++WQYVA+K+E+NLL+GI+LTGG+  
Sbjct: 241 GIVVAWKLRLVPVPTTLTTCTVNRTLDRDGVKLIHRWQYVAHKLEDNLLLGISLTGGHIL 300

Query: 301 PTKGVKRNPTASFFSLYLGKTDKLVTIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNG 360
             +G K NP ASF + +LGK D+LV I+NTTFP LGLTK +C ETSWI+STLI AGF+ G
Sbjct: 301 TQQGGKTNPVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIESTLITAGFSAG 360

Query: 361 QPLEILLTKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKM 420
           + LE LL +  L  NIS KIKSDYVK+PIS+  FKGIW+RL +Q++E +Q+   PYGG+M
Sbjct: 361 ESLETLLYRRPLV-NISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVPYGGRM 420

Query: 421 SNISSSKTPFSHRAEFLYKISYTVGWAEKGTGANERHLNWIREFYSFMTPFVSNSPRAAY 480
           S  S S+TP+ HRAE +YK+ Y V W E+   A +RHLNW+RE Y +M P+V+ SPRAAY
Sbjct: 421 SEFSESETPYPHRAENMYKMGYIVKWEEQSAKAEKRHLNWVREIYDYMAPYVTKSPRAAY 480

Query: 481 VNYRDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIP 531
           VNYRDLDIG NN+Y KT+YE A +WGLKYFG NF RLVQVKT VDPS+FFRHEQSIP
Sbjct: 481 VNYRDLDIGRNNEYEKTNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQSIP 535

BLAST of Chy12G210330.1 vs. NCBI nr
Match: XP_004135741.1 (tetrahydrocannabinolic acid synthase [Cucumis sativus] >KAE8653321.1 hypothetical protein Csa_023149 [Cucumis sativus])

HSP 1 Score: 1050 bits (2714), Expect = 0.0
Identity = 518/535 (96.82%), Postives = 524/535 (97.94%), Query Frame = 0

Query: 1   MKLFPLNPLLFISFTIVSLFSTQWSTASSNKNKDFLQCLSIHSTDIPIYTPINSSYSSIL 60
           MK FPLNPL  ISFTIVSLFSTQWSTASS KNKDFLQCLSIHST IPIYTPINSSYSSIL
Sbjct: 1   MKFFPLNPLFLISFTIVSLFSTQWSTASSYKNKDFLQCLSIHSTHIPIYTPINSSYSSIL 60

Query: 61  RSYAQNPRFSTQYKTLKPLVIIKPSRVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYIS 120
           RSYAQN RFSTQYKTLKPLVIIKPS VSHLQSTVICSKSH LQIRIRSGGHDTEGLSYIS
Sbjct: 61  RSYAQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHDLQIRIRSGGHDTEGLSYIS 120

Query: 121 DLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGG 180
           DLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVG+GG
Sbjct: 121 DLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGG 180

Query: 181 HFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVG 240
           HFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGE LFWAIRGGGGGSFGIVVG
Sbjct: 181 HFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWAIRGGGGGSFGIVVG 240

Query: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKR 300
           WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVAN+MEE LLIGINLTGGNPTKGVKR
Sbjct: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKR 300

Query: 301 NPTASFFSLYLGKTDKLVTIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360
           NPTASFFSLYLGKTDKLVTIMNTT PNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL
Sbjct: 301 NPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360

Query: 361 TKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNISSSK 420
           +KPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNISSSK
Sbjct: 361 SKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNISSSK 420

Query: 421 TPFSHRAEFLYKISYTVGWAEKGTGANERHLNWIREFYSFMTPFVSNSPRAAYVNYRDLD 480
           TPFSHRAEFLYKISYTVGWAE+G+GANERHLNWIREFYSFMTPFVSNSPRAAYVNYRDLD
Sbjct: 421 TPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYVNYRDLD 480

Query: 481 IGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPLTLS 535
           IGTNNKYGKTSYEEASIWGLKYFGNNFK+LVQVKTTVDPSNFFRHEQSIPPLTLS
Sbjct: 481 IGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHEQSIPPLTLS 535

BLAST of Chy12G210330.1 vs. NCBI nr
Match: XP_008445749.1 (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo] >KAA0047086.1 tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa] >TYK05069.1 tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1033 bits (2670), Expect = 0.0
Identity = 507/535 (94.77%), Postives = 519/535 (97.01%), Query Frame = 0

Query: 1   MKLFPLNPLLFISFTIVSLFSTQWSTASSNKNKDFLQCLSIHSTDIPIYTPINSSYSSIL 60
           MK FPLNPLLF SFT+VSL STQWSTASS KNKDFL+CLSIHSTDIPIYTPINSSY+S L
Sbjct: 1   MKSFPLNPLLFTSFTVVSLSSTQWSTASSYKNKDFLRCLSIHSTDIPIYTPINSSYTSTL 60

Query: 61  RSYAQNPRFSTQYKTLKPLVIIKPSRVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYIS 120
           RSYAQNPRFSTQYKTLKPL IIKPS VSH+QSTVICSKSHGLQIRIRSGGHDTEGLSYIS
Sbjct: 61  RSYAQNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEGLSYIS 120

Query: 121 DLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGG 180
           DLPF+VVDLINLKSIEVDTTNNT WVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGG
Sbjct: 121 DLPFIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGG 180

Query: 181 HFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVG 240
            FSGGGYGWLMRKYGLAADNVIDAYLVDANG VFD EAMGEGLFWAIRGGGGGSFGIVVG
Sbjct: 181 QFSGGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGGGGGSFGIVVG 240

Query: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKR 300
           WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKR
Sbjct: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKR 300

Query: 301 NPTASFFSLYLGKTDKLVTIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360
           NPTASFFSLYLGKTDKL+ IMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL
Sbjct: 301 NPTASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360

Query: 361 TKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNISSSK 420
           TKPTLSNNISYKIKSDYVKQPIS+HAFKGIWDRL+S EVETSQLFLFPYGGKM+NISSSK
Sbjct: 361 TKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPYGGKMNNISSSK 420

Query: 421 TPFSHRAEFLYKISYTVGWAEKGTGANERHLNWIREFYSFMTPFVSNSPRAAYVNYRDLD 480
           TPFSHRAEFLYKISYTVGWAE+GTGANERHLNWIRE YSFMTPFVSNSPRAAYVNYRDLD
Sbjct: 421 TPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSNSPRAAYVNYRDLD 480

Query: 481 IGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPLTLS 535
           IGTNNK GKTSYEEASIWG+KYFGNNFKRLVQVKT VDPSNFFRH+QSIPPLTLS
Sbjct: 481 IGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSIPPLTLS 535

BLAST of Chy12G210330.1 vs. NCBI nr
Match: XP_038895165.1 (tetrahydrocannabinolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 940 bits (2430), Expect = 0.0
Identity = 459/536 (85.63%), Postives = 496/536 (92.54%), Query Frame = 0

Query: 1   MKLFPLNPLL-FISFTIVSLFSTQWSTASSNKNKDFLQCLSIHSTDIPIYTPINSSYSSI 60
           MK+FPLNPLL F +F IVS F  QW+ ASS K++DFLQCLSI+S DIPIYTPINSSYSS+
Sbjct: 1   MKVFPLNPLLTFTNFIIVSSFLVQWTAASSYKHEDFLQCLSIYSRDIPIYTPINSSYSSL 60

Query: 61  LRSYAQNPRFSTQYKTLKPLVIIKPSRVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYI 120
           L SYAQN RFSTQ+KTLKPLVIIKPS +SH+Q+ VICSKSHGLQIRIRSGGHDTEGLSYI
Sbjct: 61  LTSYAQNTRFSTQHKTLKPLVIIKPSHISHIQAIVICSKSHGLQIRIRSGGHDTEGLSYI 120

Query: 121 SDLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVG 180
           SD+PF+V+DLINLKSIEVDT NNTAWVQSGATIGELYY+IAEKSRTLAFPAGVCPTVG+G
Sbjct: 121 SDVPFIVIDLINLKSIEVDTKNNTAWVQSGATIGELYYKIAEKSRTLAFPAGVCPTVGIG 180

Query: 181 GHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVV 240
           GHFSGGGYGWLMRKYGLAADNV+DAYLVD +G VFDRE MGE LFWAIRGGGGGSFGIVV
Sbjct: 181 GHFSGGGYGWLMRKYGLAADNVMDAYLVDDDGRVFDRETMGEDLFWAIRGGGGGSFGIVV 240

Query: 241 GWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVK 300
           GWKVKLVRVPPIVTTCRL+KSLDKNTKKIVYQWQYVANKME+ LLIGINLTGGNPT+GVK
Sbjct: 241 GWKVKLVRVPPIVTTCRLEKSLDKNTKKIVYQWQYVANKMEDTLLIGINLTGGNPTQGVK 300

Query: 301 RNPTASFFSLYLGKTDKLVTIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEIL 360
           RNPTASFFSL+LGKT+KL+TIMNTTFP LGLTK+NC+ETSWIQSTLI+AGFTNG+ LE+L
Sbjct: 301 RNPTASFFSLFLGKTNKLITIMNTTFPELGLTKSNCEETSWIQSTLISAGFTNGEALEVL 360

Query: 361 LTKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNISSS 420
           L KP +   ISYKIKSDYVKQPISQ A KGIW+RLKSQ  ETSQLFLFPYGGKMS ISSS
Sbjct: 361 LAKPPIKT-ISYKIKSDYVKQPISQQALKGIWERLKSQVTETSQLFLFPYGGKMSKISSS 420

Query: 421 KTPFSHRAEFLYKISYTVGWAEKGTGANERHLNWIREFYSFMTPFVSNSPRAAYVNYRDL 480
           K PFSHRAEFLYKISYTVGW E+   ANERHLNWIRE Y +MTPFVSNSPRAAY+NYRDL
Sbjct: 421 KIPFSHRAEFLYKISYTVGWTEQSIVANERHLNWIRELYDYMTPFVSNSPRAAYINYRDL 480

Query: 481 DIGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPLTLS 535
           DIGTNNKYG TSY++ASIWGLKYFGNNFKRLVQVKT VDPSNFFRHEQSIPPL+LS
Sbjct: 481 DIGTNNKYGNTSYKQASIWGLKYFGNNFKRLVQVKTKVDPSNFFRHEQSIPPLSLS 535

BLAST of Chy12G210330.1 vs. NCBI nr
Match: XP_023520034.1 (tetrahydrocannabinolic acid synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 790 bits (2041), Expect = 7.79e-284
Identity = 388/523 (74.19%), Postives = 442/523 (84.51%), Query Frame = 0

Query: 16  IVSLFSTQWSTASSNKNKDFLQCLSIHSTDIP--IYTPINSSYSSILRSYAQNPRFSTQY 75
           I+S FS  W  A ++K++ FLQCLSIHS DI   IYTPINSSYSS+L SYA NPR ST  
Sbjct: 11  ILSSFSVLWVVALAHKHEHFLQCLSIHSRDISKVIYTPINSSYSSLLSSYALNPRVSTP- 70

Query: 76  KTLKPLVIIKPSRVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYISD-LPFVVVDLINL 135
            T  P VIIKPS +SH+Q+ V+CSKSHG +IR RSGGHD EGLSYIS  LPF+VVDL NL
Sbjct: 71  ATPNPRVIIKPSHLSHIQAAVVCSKSHGFRIRTRSGGHDAEGLSYISHHLPFIVVDLKNL 130

Query: 136 KSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGGHFSGGGYGWLMR 195
            SI VD  NNTAWVQSGATIGE+YY IA+KSRTLAFPAGVCPTVG GGH SGGGYGWLMR
Sbjct: 131 NSIAVDIENNTAWVQSGATIGEVYYEIAKKSRTLAFPAGVCPTVGFGGHLSGGGYGWLMR 190

Query: 196 KYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVGWKVKLVRVPPIV 255
           KYGLAADNV+DAYLVDA G V DREAMGE LFWAIRGGGGGSFGIVVGWKVKLV VP IV
Sbjct: 191 KYGLAADNVVDAYLVDAYGKVLDREAMGEDLFWAIRGGGGGSFGIVVGWKVKLVAVPAIV 250

Query: 256 TTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKRNPTASFFSLYLG 315
           TTC+L+K+LD   KK+VYQWQYVANKM+ENLL+G+NLTGGNPT+G K N  A+FFSL+LG
Sbjct: 251 TTCQLEKNLDGYAKKLVYQWQYVANKMDENLLLGVNLTGGNPTEGAKPNHKATFFSLFLG 310

Query: 316 KTDKLVTIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILLTKPTLSNNISYK 375
            + +L+  MNTTFP LGLT  +C++TSWIQSTL+AAGFTN  PLE LLTK T  N ++YK
Sbjct: 311 NSHQLIATMNTTFPQLGLTNNDCQQTSWIQSTLLAAGFTNRDPLEFLLTK-TSFNTVAYK 370

Query: 376 IKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNISSSKTPFSHRAEFLYK 435
           IKSDYV +PIS+HAFKGIWDRLKS ++E++QL LFPYGG+MS I + +TPFSHRAEF+YK
Sbjct: 371 IKSDYVNKPISKHAFKGIWDRLKSPKMESAQLLLFPYGGRMSQIPTWETPFSHRAEFMYK 430

Query: 436 ISYTVGWAEKGTGANERHLNWIREFYSFMTPFVSNSPRAAYVNYRDLDIGTNNKYGKTSY 495
           ISY VGWAE+   ANER+LNW+REFY +M+PFVSNSPRAAYVNYRDLDIG NN+YG TSY
Sbjct: 431 ISYVVGWAEQSMEANERNLNWVREFYEYMSPFVSNSPRAAYVNYRDLDIGMNNEYGSTSY 490

Query: 496 EEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPLTLS 535
           EEA +WG  YFG+NFKRLV+VK  VDP NFFRHEQS+PPLTLS
Sbjct: 491 EEARVWGEMYFGDNFKRLVRVKNKVDPLNFFRHEQSVPPLTLS 531

BLAST of Chy12G210330.1 vs. NCBI nr
Match: XP_022927076.1 (berberine bridge enzyme-like 18 [Cucurbita moschata])

HSP 1 Score: 780 bits (2015), Expect = 7.03e-280
Identity = 382/523 (73.04%), Postives = 440/523 (84.13%), Query Frame = 0

Query: 16  IVSLFSTQWSTASSNKNKDFLQCLSIHSTDIP--IYTPINSSYSSILRSYAQNPRFSTQY 75
           I+S F+  W+ A ++K++ FLQCLSIHS DI   IYTPINSSYSS+L SYA NPR S   
Sbjct: 11  ILSSFTVLWAAALAHKHEHFLQCLSIHSRDISKLIYTPINSSYSSLLTSYALNPRVSIP- 70

Query: 76  KTLKPLVIIKPSRVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYISD-LPFVVVDLINL 135
            T  P VIIKPS +SH+Q+ V+CSKSHG QIR RSGGHD EGLSYIS  LPF++VDL NL
Sbjct: 71  ATPNPHVIIKPSHLSHIQAAVVCSKSHGFQIRTRSGGHDAEGLSYISHHLPFIIVDLKNL 130

Query: 136 KSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGGHFSGGGYGWLMR 195
            SI V+  NNTAWVQSGATIGE+YY IA+KSRTLAFPAGVCPTVG GGH SGGGYGWLMR
Sbjct: 131 NSITVNIENNTAWVQSGATIGEVYYEIAKKSRTLAFPAGVCPTVGFGGHLSGGGYGWLMR 190

Query: 196 KYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVGWKVKLVRVPPIV 255
           KYGLAADNV+DAYLVDA G V DREAMGE LFWAIRGGGGGSFGIVVGWKVKLV VP IV
Sbjct: 191 KYGLAADNVVDAYLVDAYGKVLDREAMGEDLFWAIRGGGGGSFGIVVGWKVKLVAVPAIV 250

Query: 256 TTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKRNPTASFFSLYLG 315
           TTC+L+K+LD   KK+VYQWQYVANKM+ENLL+G+NLTGGNPT+G K N  A+FFSL+LG
Sbjct: 251 TTCQLEKNLDGYAKKLVYQWQYVANKMDENLLLGVNLTGGNPTQGAKPNHKATFFSLFLG 310

Query: 316 KTDKLVTIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILLTKPTLSNNISYK 375
            +  L+  MNTTFP LGLT  +C++TSWIQSTL+AAGFT+  PLE LLTK T  N ++YK
Sbjct: 311 NSHDLIATMNTTFPQLGLTNNDCQQTSWIQSTLLAAGFTSRDPLEFLLTK-TSFNTVAYK 370

Query: 376 IKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNISSSKTPFSHRAEFLYK 435
           IKSDYV +PIS+HAFKGIWDRLKS ++E++QL LFPYGG+MS I + +TPFSHRAEF+YK
Sbjct: 371 IKSDYVNKPISKHAFKGIWDRLKSPKMESAQLLLFPYGGRMSKIPTWETPFSHRAEFMYK 430

Query: 436 ISYTVGWAEKGTGANERHLNWIREFYSFMTPFVSNSPRAAYVNYRDLDIGTNNKYGKTSY 495
           ISY VGWAE+   A+ER+LNW+REFY +M+PFVSNSPRAAYVNYRDLDIG NN+YG TSY
Sbjct: 431 ISYVVGWAEQSREASERNLNWVREFYEYMSPFVSNSPRAAYVNYRDLDIGMNNEYGSTSY 490

Query: 496 EEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPLTLS 535
           EEA +WG  YFG+NFKRLV+VK  VDP NFFRHEQS+PPL LS
Sbjct: 491 EEARVWGEMYFGDNFKRLVRVKNKVDPLNFFRHEQSVPPLALS 531

BLAST of Chy12G210330.1 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 495.4 bits (1274), Expect = 5.9e-140
Identity = 258/529 (48.77%), Postives = 347/529 (65.60%), Query Frame = 0

Query: 14  FTIVSLFSTQWS---TASSNKNKDFLQCLSIHSTDIP-----IYTPINSSYSSILRSYAQ 73
           FTI+ LFS   S   +A  + ++DFL+CLS    D       I+T  +SS+ SIL S  Q
Sbjct: 8   FTIL-LFSLNISPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSSIQ 67

Query: 74  NPRFSTQYKTLKPLVIIKPSRVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYIS-DLPF 133
           NPRFS   +T KP+ II P + S +Q+ + C++ HG+ +R RS GH  EGLSYI+ + PF
Sbjct: 68  NPRFSVS-ETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPF 127

Query: 134 VVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGGHFSG 193
            V+DL NL+SI +D  N T WVQ+GAT GELYY I + +++LAFPAG+ PTVGVGG FSG
Sbjct: 128 AVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSG 187

Query: 194 GGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVGWKVK 253
           GGYG L+RKYGLAADN+IDA +VDA+G + DR+AMGE  FWAIRGGGG SFG+++ WKVK
Sbjct: 188 GGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVK 247

Query: 254 LVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKRNPTA 313
           LV VP  +T  ++ K+  K   +I+ +WQY A+K+ ++L I   L   N     K    A
Sbjct: 248 LVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSN-----KNAVHA 307

Query: 314 SFFSLYLGKTDKLVTIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILLTKPT 373
            F  LY+G  + L+ +M   FP LGL K  C+E SWI+S L  A F  G+ L +L  +  
Sbjct: 308 LFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADFPKGESLGVLTNRER 367

Query: 374 LSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNISSSKTPFS 433
            S  +S+K K D+V++PI + A + IW RL++ E    ++ L P+GGKMS ++  +TPF 
Sbjct: 368 TS--LSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPFP 427

Query: 434 HRAEFLYKISYTVGWAE---KGTGANERHLNWIREFYSFMTPFVSNSPRAAYVNYRDLDI 493
           HR   LY+I Y   W E   K     +++L W+   Y FMTP+VS SPR AYVN++D+D+
Sbjct: 428 HRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKDMDL 487

Query: 494 GTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIP 531
           G      KT YEE   WG+KYF NNF+RLV+VKT VDP++FF  EQSIP
Sbjct: 488 GMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of Chy12G210330.1 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 490.3 bits (1261), Expect = 1.9e-138
Identity = 259/531 (48.78%), Postives = 351/531 (66.10%), Query Frame = 0

Query: 10  LFISFTIVSLFSTQWSTASSNK--NKDFLQCLS--IHSTDIP-IYTPINSSYSSILRSYA 69
           LFI F +++L  + + + S+ +  +++FL+CLS  I+  D   I+T  + SY SIL S  
Sbjct: 10  LFIYFLLLNLSLSHFPSISAQRTNHENFLKCLSHRINEDDSRIIHTSKDPSYFSILNSSI 69

Query: 70  QNPRFSTQYKTLKPLVIIKPSRVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYIS-DLP 129
           QNPRF    +T KP+ II P + + +QST+ C++ HG+ IR RSGGHD EGLSY++   P
Sbjct: 70  QNPRFFV-LETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRP 129

Query: 130 FVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGGHFS 189
           FVV+DL NL+SI +D  N T WVQSGATIGELYY I + S++LAFPAG+ PTVG+GG F 
Sbjct: 130 FVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFG 189

Query: 190 GGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVGWKV 249
           GGGYG LMRKYGL+ADNVIDA++VDANG   DR+ MGE  FWAIRGGGG SF +V+ WK+
Sbjct: 190 GGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKI 249

Query: 250 KLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKRNPT 309
           +L+ VP +VT  ++ K+ +K    I+ +WQY+A+K+  +L I   L         +    
Sbjct: 250 RLLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAMLQ-------KETEVY 309

Query: 310 ASFFSLYLGKTDKLVTIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILLTKP 369
           ASF  LYLG    L+ +M   FP LGL   NC+E SWI+S L    F  G+ +EIL  + 
Sbjct: 310 ASFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVL---WFIKGESMEILAKRK 369

Query: 370 TLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNISSSKTPF 429
             S   S+K K D++++PI + A + +W R ++ E   +++ L P+GGKMS I+ ++ PF
Sbjct: 370 RTSR--SFKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPF 429

Query: 430 SHRAEFLYKISYTVGWAE---KGTGANERHLNWIREFYSFMTPFVSNSPRAAYVNYRDLD 489
            HR   LY+I Y   W+E   K     E++L W+   Y FMTP+VS SPR AYVN+RD+D
Sbjct: 430 PHREGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDID 489

Query: 490 IGTNNKYG-KTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIP 531
           +G       KT YEEA +WG+KYF NNF RLV+VKT VDP +FF  EQSIP
Sbjct: 490 LGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIP 527

BLAST of Chy12G210330.1 vs. TAIR 10
Match: AT5G44360.1 (FAD-binding Berberine family protein )

HSP 1 Score: 488.4 bits (1256), Expect = 7.3e-138
Identity = 254/535 (47.48%), Postives = 358/535 (66.92%), Query Frame = 0

Query: 13  SFTIVSLFSTQW--STASSNKNKD-FLQCLSIHSTD------IPIYTPINSSYSSILRS- 72
           + ++  +F  +W  S +SS+ +KD FL C+S HS          I+ P +  Y+   +S 
Sbjct: 8   ALSLFLVFLVKWVNSDSSSSPSKDQFLSCMSTHSDSSFINPKSFIHKPDSRVYTDFSQSL 67

Query: 73  YAQNPRFST-QYKTLKPLVIIKPSRVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYIS- 132
            +QN RF T  + + KP++I+ P   + +Q +++CS+  G+++R +SGGHD EGLSY+S 
Sbjct: 68  ISQNYRFLTLNFTSQKPILIVTPRTDTEIQRSLLCSRKLGVKVRTKSGGHDYEGLSYLSL 127

Query: 133 DLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGG 192
             PF+++DL+N++SIE++  + TAWV +GATIGELYY+IA+ S+   FPAG CP+VGVGG
Sbjct: 128 HSPFIILDLVNVRSIEINLADETAWVGAGATIGELYYKIAKSSKIHGFPAGTCPSVGVGG 187

Query: 193 HFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFD-REAMGEGLFWAIRGGGGGSFGIVV 252
           HFSGGG+G +MRK+GLAADNV+DA  VDANG +++ R  MGE LFWAIRGGG  SFG+V+
Sbjct: 188 HFSGGGFGAMMRKHGLAADNVVDARFVDANGRIYNSRREMGEDLFWAIRGGGAASFGVVL 247

Query: 253 GWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIG--INLTGGNPTKG 312
            WKVKLVRVP  VT  R +  L +N  KIV++WQ +A ++++NL I   ++++GG     
Sbjct: 248 SWKVKLVRVPEKVTCFRRNLPLTQNMTKIVHRWQQIAAELDDNLFIRVIVSISGG----- 307

Query: 313 VKRNPTASFFSLYLGKTDKLVTIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLE 372
              +   +F + YLG  DKL+ +MN  FP LGLT  +C E +WI S ++   +  GQPLE
Sbjct: 308 ---SVQTTFQANYLGGIDKLIPLMNQKFPELGLTFQDCSEMTWIDS-IMYFNWKKGQPLE 367

Query: 373 ILLTKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNIS 432
            LL +    N++ +K KSD+VK PI +   +GIW R    EVE+  + + P GGKM  I 
Sbjct: 368 TLLDRGQRYNDLYFKAKSDFVKNPIPEIGLEGIWTRF--HEVESPIMIMEPLGGKMYEIG 427

Query: 433 SSKTPFSHRAEFLYKISYTVGWAEKGTGANERHLNWIREFYSFMTPFVSNSPRAAYVNYR 492
            ++TPF HR   LY I Y V W  K  G  E+H+ W+R  Y +M  +VS SPR AY+NYR
Sbjct: 428 ETETPFPHRRGNLYNIQYMVKWRLKDIGVMEKHVTWMRLLYRYMRVYVSASPRGAYLNYR 487

Query: 493 DLDIGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPL 533
           DLD+G N     TS+E+A +WG +YFG+NFKRL  VK  +DP+NFFR+EQS+PPL
Sbjct: 488 DLDLGMNRGV-NTSFEDAKLWGFRYFGSNFKRLAIVKGKIDPTNFFRNEQSVPPL 530

BLAST of Chy12G210330.1 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 485.3 bits (1248), Expect = 6.2e-137
Identity = 252/530 (47.55%), Postives = 349/530 (65.85%), Query Frame = 0

Query: 9   LLFISFTIVSLFSTQWSTASSNKNKDFLQCLSIHSTDI-----PIYTPINSSYSSILRSY 68
           L+F + + + L      +A+ +    FLQCLS+   D       I+TP ++S+SS+L S 
Sbjct: 11  LIFFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASS 70

Query: 69  AQNPRFSTQYKTLKPLVIIKPSRVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYISDLP 128
            QN RFS      KP++I+ P + S +QS V C++  G+ IR RSGGHD EGLSY++  P
Sbjct: 71  IQNQRFSAP-DVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKP 130

Query: 129 FVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGGHFS 188
           FV++DL NL+SI VD  N + WVQ+GATIGELYY I +K+RTLAFPAGVCPTVGVGGHFS
Sbjct: 131 FVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFS 190

Query: 189 GGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVGWKV 248
           GGGYG L+RK+GLAAD+VIDA +VDA G + +R  MGE  FWAIRGGGG SF +V+ WK+
Sbjct: 191 GGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKI 250

Query: 249 KLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKRNPT 308
            L+ VP  VT   + K  +++  KI+++WQ+VA+K+ ++L I + L      +  K    
Sbjct: 251 GLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVML------QRYKNMVR 310

Query: 309 ASFFSLYLGKTDKLVTIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILLTKP 368
           ASF  LYLG    L+ ++N  FP LGL + +C E SWI+S +  A     +P+ + LTK 
Sbjct: 311 ASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAEL-GEEPINV-LTKR 370

Query: 369 TLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNISSSKTPF 428
           T + ++++K KSD+V++P+ + A   +W RL+  E E +QL   P+GGKMS I+  +TPF
Sbjct: 371 TRA-SLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPF 430

Query: 429 SHRAEFLYKISYTVGWAEKGTGANERHLNWIREFYSFMTPFVSNSPRAAYVNYRDLDIGT 488
            HR   +Y+I Y   W        E+++ W+   Y  M+ FV+ SPR AY+N RDLD+G 
Sbjct: 431 PHRKGNIYEIQYLNYWR---GDVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGM 490

Query: 489 NNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPLT 534
                ++ YEE   WG+KYF NNF+RLV+VKT+VDPS+FF  EQSIPP T
Sbjct: 491 YVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIPPFT 527

BLAST of Chy12G210330.1 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 481.1 bits (1237), Expect = 1.2e-135
Identity = 246/542 (45.39%), Postives = 344/542 (63.47%), Query Frame = 0

Query: 8   PLLFISFTIVSLFSTQWSTASSNKNKDFLQCLSIHSTDIPIYTPIN----------SSYS 67
           PL  I     SL++   +++ ++    F+ C+     +  +Y P+           S +S
Sbjct: 8   PLFSILVLYFSLYTITPTSSLASLQDQFINCV---QRNTHVYFPLEKTFFAPTKNVSMFS 67

Query: 68  SILRSYAQNPRFSTQYKTLKPLVIIKPSRVSHLQSTVICSKSHGLQIRIRSGGHDTEGLS 127
            +L S AQN RF  +    KP  I  P   SH+Q+++ICSK   + +R+RSGGHD EGLS
Sbjct: 68  QVLESTAQNLRF-LKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLS 127

Query: 128 YIS--DLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPT 187
           Y+S  D PF+++DL  ++ + ++  +N+AWVQSGAT+GELYYRIAEKS+   FPAG+C +
Sbjct: 128 YVSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSS 187

Query: 188 VGVGGHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSF 247
           +G+GGH +GG YG +MRKYGL ADNV+DA +VDANG + DR AMGE  FWAIRGG GGSF
Sbjct: 188 LGIGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSF 247

Query: 248 GIVVGWKVKLVRVPPIVTTCRLDKSLDKNT-KKIVYQWQYVANKMEENLLIGINL----T 307
           GI++ WK+KLV VP  VT   + K+L ++   KI+ +WQ VA+K+ E L I +      T
Sbjct: 248 GIILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGT 307

Query: 308 GGNPTKGVKRNPTASFFSLYLGKTDKLVTIMNTTFPNLGLTKANCKETSWIQSTLIAAGF 367
           GGN T       T S+ +L+LG    L+ +M  +FP LGLT  +C E SW++S    +GF
Sbjct: 308 GGNKT------VTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGF 367

Query: 368 TNGQPLEILLTKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYG 427
               P  +LL   +    +S+K KSD+VK PI +   +GI+ +L  +++    +   PYG
Sbjct: 368 PTHTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPL--MIWNPYG 427

Query: 428 GKMSNISSSKTPFSHRAEFLYKISYTVGWAEKGTGANERHLNWIREFYSFMTPFVSNSPR 487
           G M+ I  S+ PF HR   L+K+ Y   W +     + RH+NWIR+ YS+MTP+VS++PR
Sbjct: 428 GMMAKIPESQIPFPHRKGVLFKVQYVTSWLDSDKRPS-RHINWIRDLYSYMTPYVSSNPR 487

Query: 488 AAYVNYRDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIP 533
            AYVNYRDLD+G N K  KT  ++A +WG  YF NNF RL+ +K  VDP NFFRHEQSIP
Sbjct: 488 EAYVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIP 536