Chy12G209840.1 (mRNA) Cucumber (hystrix) v1

BLAST of Chy12G209840.1 vs. ExPASy Swiss-Prot
Match: F4IP69 (Sec1 family domain-containing protein MIP3 OS=Arabidopsis thaliana OX=3702 GN=MIP3 PE=1 SV=1)

HSP 1 Score: 950.7 bits (2456), Expect = 1.2e-275
Identity = 509/870 (58.51%), Postives = 637/870 (73.22%), Query Frame = 0

Query: 1   MALLDVTRSCLDSISQIADHLKGSILYLDAGCVESFQILGGFPLLLDHGVNVVCSLENMA 60
           MAL+DV  SCL+SI +I + +K +I+Y+DAGC ESFQ +G FPL L+ G   VCSLENM 
Sbjct: 1   MALIDVAISCLNSIREIEEDVKDAIVYIDAGCTESFQFVGAFPLFLELGARAVCSLENMT 60

Query: 61  SLDAVIDWNPAS--ATKLVVITSRLLSDAHRYILRCLTTHQDVRHCTIFTSISEIAQSVY 120
           SLDAV DWN  S  A ++V++TSRLL+DAHRY+LRCL+TH+ V+ CT+FTSISE + S  
Sbjct: 61  SLDAVADWNSKSDCAKRIVIMTSRLLNDAHRYMLRCLSTHEGVQRCTVFTSISEGSHSAI 120

Query: 121 PDSPLGPDAFHEYESLLVQDYEELVKKDEKRALPSEDKNLEKCISSEDEGWSRLTSSEED 180
           PDSPLGPDA+ EYE+LLVQDY E  KK +K               S+D+G S+ +S+ E 
Sbjct: 121 PDSPLGPDAYREYETLLVQDYNEHTKKSDK--------------ISKDKGVSKFSSALES 180

Query: 181 ITQVEASSSGRDSYEDVLTSHRQDVGKKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEAC 240
           +T     S       D+ +   Q     LVVSVHHFP+I+CPF+PR FVLPS+G +AEA 
Sbjct: 181 LTMEPIESEN----VDISSGGAQG----LVVSVHHFPLIICPFTPRAFVLPSQGSVAEAS 240

Query: 241 LSAENVDSLSPGLPPLYTGMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDLSKT 300
           LS ++ DSLS GLPP+ TG   D DD+PPGATLTAHFLY  A KM+LK+EIFS+GD SK 
Sbjct: 241 LSRQHEDSLSFGLPPISTGSMSDTDDVPPGATLTAHFLYQLALKMELKLEIFSLGDQSKN 300

Query: 301 VGKILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRTSPVT 360
           VGKILTDMSS+YDV RRK+SAGLLLVDRTLDL+TPCCHGDSL DR+F SLPR +R S   
Sbjct: 301 VGKILTDMSSVYDVARRKRSAGLLLVDRTLDLITPCCHGDSLFDRIFSSLPRAERFS--- 360

Query: 361 HVKGPETSLKKGPRICRRAPLDVRIPFAEILTEDEGKADKFRLGERIEAFLSGWNSGNST 420
                +  LK+G     R  LDV++P  E+L E+  K     L E IEAFL GW+S  S 
Sbjct: 361 ----SQAQLKQGVPSINRPSLDVQVPLGELLNEEPSKIRDSGLPEGIEAFLRGWDSYTSA 420

Query: 421 SQNFNKGGESNRDQTLQSPIYDPELLSGCFVSSENFRGTPYMEAILDRKTKDGTVLIKKW 480
            QN     E ++  T        ELL+G  V++E FRGTPY+EA++DRKTKDG+VL+KKW
Sbjct: 421 PQNVGLFNECDKKSTTNW----TELLNGSLVATECFRGTPYLEAMIDRKTKDGSVLVKKW 480

Query: 481 LQETMRKENVAVNGKIRPGFPTKLELESMIKALAKSQTCLLRNKGVLQLAAAATVAIEEL 540
           LQE +R+EN++VN + RPG+ TK EL++MIKAL++SQ+ LL+NKG++QL AA   A++E 
Sbjct: 481 LQEALRRENISVNVRARPGYATKPELQAMIKALSQSQSSLLKNKGIIQLGAATAAALDES 540

Query: 541 NSTRWDAFLSAEKILRASAEDTSQGLAAQIVDLINKSVL----VVKSE----ASKGVLSF 600
            S +WD F SAE +L  SA DTSQGLAAQI DLINKS +      K+E    +S+G+LSF
Sbjct: 541 QSAKWDTFSSAEMMLNVSAGDTSQGLAAQISDLINKSAVAELQAKKNEKPDSSSRGLLSF 600

Query: 601 EDALLLTITGYILAGENFPTSGSDGPFSWQEEHFIKEAIIDAILENPVDGKLKFLHGLIE 660
            DALLLTI GYILAGENFPTSGS GPFSWQEEHF+KEAI+DA+LENP  G LKFL+GL E
Sbjct: 601 RDALLLTIVGYILAGENFPTSGSGGPFSWQEEHFLKEAIVDAVLENPSAGNLKFLNGLTE 660

Query: 661 ELQTNRDRMISKGTKEMGSSQIKDDDF--DDQWESWGDDDTD--INTTNEEVYDDMQLKL 720
           EL+   +R+ S+ TKE+ S    D D   DD W  WGD++ +   N+  +E YDDMQLKL
Sbjct: 661 ELEGRLNRLKSEETKEIPSDDQLDIDALDDDPWGKWGDEEEEEVDNSKADESYDDMQLKL 720

Query: 721 ELRDRVDSLFKTLHKLSGTKKINLLLKE-TLNSENILNGDQYANKGVLYKLLARILNKHD 780
           +LRDRVDSLF+ LHKLS  +  NL L+E +L SE+   G+   NKG++Y+L+ ++L+K +
Sbjct: 721 DLRDRVDSLFRFLHKLSSLRTRNLPLREGSLASESSFPGEPSGNKGLVYRLITKVLSKQE 780

Query: 781 LPNLEYHSSTMGRLFKSGFGRFGLGQAKPSLADQNVILVFVIGGINGLEVREAQEALSES 840
           +P LEYHSST+GR  KSGFGRFGLGQAKPSLADQ+VILVFVIGGING+EV EAQEA+SES
Sbjct: 781 IPGLEYHSSTVGRFIKSGFGRFGLGQAKPSLADQSVILVFVIGGINGIEVLEAQEAVSES 837

Query: 841 GRPDIELIVGGTTFLTPHDMFDLLLGDSAY 856
           GRPDI L++GGTT LTP DMF+LLLG  ++
Sbjct: 841 GRPDINLVIGGTTLLTPDDMFELLLGQFSH 837

BLAST of Chy12G209840.1 vs. ExPASy Swiss-Prot
Match: Q8WU76 (Sec1 family domain-containing protein 2 OS=Homo sapiens OX=9606 GN=SCFD2 PE=1 SV=2)

HSP 1 Score: 82.0 bits (201), Expect = 3.5e-14
Identity = 96/411 (23.36%), Postives = 182/411 (44.28%), Query Frame = 0

Query: 446 GCFVSSENFRGTPYMEAILDRKTKDGTVLIKKWLQETMRKENVAVN---GKIRPGFPTKL 505
           GC   S +       EA+L+ K K+  + +++ L E   +EN+ +    G++ PG     
Sbjct: 330 GCLSQSSDTTAKALWEALLNTKHKEAVMEVRRHLVEAASRENLPIKMSMGRVTPG----- 389

Query: 506 ELESMIKALAKSQTCLLRNKGVLQLAAAATVAIEELNSTRWDAFLSAEKILRASAEDTSQ 565
           +L S I+    +   L+ + G+LQL  A    ++   + +WD FL+ E++L  S  +++ 
Sbjct: 390 QLMSYIQLFKNNLKALMNHCGLLQLGLATAQTLKHPQTAKWDNFLAFERLLLQSIGESAM 449

Query: 566 GLAAQIVDLINKSVLVVK--SEASKGVLSFEDALLLTITGYILAGENFPTSGSDGPFSWQ 625
            +      ++N+ + ++K  ++ +    S E+ L+L I  Y + GE   T   D     +
Sbjct: 450 SV------VLNQLLPMIKPVTQRTNEDYSPEELLILLIYIYSVTGE--LTVDKD---LCE 509

Query: 626 EEHFIKEAIIDAILENPVDGKLKFLHGLIEELQTNRDRMISKGTKEMGSSQIKDDDFDDQ 685
            E  +K+A+     E           GL   LQ   D                       
Sbjct: 510 AEEKVKKALAQVFCEE---------SGLSPLLQKITD----------------------- 569

Query: 686 WESWGDDDTDINTTNEEVYDDMQLKLELRDRVDSLFKTLHKLSGTKKINLLLKETLNSEN 745
              W   D+ IN T            + +  VD LF +L  ++G + +    K      N
Sbjct: 570 ---W---DSSINLTFH----------KSKIAVDELFTSLRDIAGARSLLKQFKSVYVPGN 629

Query: 746 ILNGDQYANKGVLYKLLARILN--KHDLPNLEYHSSTMGRLFKSGFGRFGLGQAKPSLAD 805
             +  Q + K +L +++  I +  + D  ++E+ SS +  L K+GF  F +  ++P  +D
Sbjct: 630 --HTHQASYKPLLKQVVEEIFHPERPDSVDIEHMSSGLTDLLKTGFSMF-MKVSRPHPSD 672

Query: 806 QNVILVFVIGGINGLEVREAQEALSESGRPDIELIVGGTTFLTPHDMFDLL 850
             ++++FV+GG+   EV+  ++ L  S +P  ++IV  T  L P ++ +LL
Sbjct: 690 YPLLILFVVGGVTVSEVKMVKD-LVASLKPGTQVIVLSTRLLKPLNIPELL 672

BLAST of Chy12G209840.1 vs. ExPASy Swiss-Prot
Match: Q8BTY8 (Sec1 family domain-containing protein 2 OS=Mus musculus OX=10090 GN=Scfd2 PE=1 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 6.0e-14
Identity = 96/412 (23.30%), Postives = 178/412 (43.20%), Query Frame = 0

Query: 446 GCFVSSENFRGTPYMEAILDRKTKDGTVLIKKWLQETMRKENVAVN---GKIRPGFPTKL 505
           GC   S         EA+L+ K K+  + +++ L E   +EN+ +    G++ PG     
Sbjct: 330 GCLAPSNEASAKALWEALLNSKHKEAVMEVRRHLVEAASRENLPIKMSMGRVTPG----- 389

Query: 506 ELESMIKALAKSQTCLLRNKGVLQLAAAATVAIEELNSTRWDAFLSAEKILRASAED-TS 565
           +L S I+    +   L  + G+LQL  A    ++   + +WD FL+ E++L  S  D T 
Sbjct: 390 QLMSYIQLFKNNLRALRNHCGLLQLGMATVQTLKHPQTAKWDNFLAFERLLLQSLGDSTM 449

Query: 566 QGLAAQIVDLINKSVLVVKSEASKGVLSFEDALLLTITGYILAGENFPTSGSDGPFSWQE 625
            G+  Q++ +I  S     S+ +   L+ E+ L+L I  Y + G                
Sbjct: 450 AGVLNQLLPMIKSS-----SQRTSDDLNPEELLILLIYIYSVPG---------------- 509

Query: 626 EHFIKEAIIDAILENPVDGKLK--FLHGLIEELQTNRDRMISKGTKEMGSSQIKDDDFDD 685
                +  +D  L   V+ K+K    H L EE + +   ++ K T               
Sbjct: 510 -----DVTLDRDL-GDVEEKVKKALAHVLSEESELS--PLLQKIT--------------- 569

Query: 686 QWESWGDDDTDINTTNEEVYDDMQLKLELRDRVDSLFKTLHKLSGTKKINLLLKETLNSE 745
                 D   D+     ++             V+ +F  L +++G + +    K      
Sbjct: 570 ----GCDSAVDLTLPKSQI------------AVNDVFMALREIAGARNLMRQFKSVYVPG 629

Query: 746 NILNGDQYANKGVLYKLLARILN--KHDLPNLEYHSSTMGRLFKSGFGRFGLGQAKPSLA 805
           N  N  Q + K +L +++  I N  K D  ++E+ SS +  L K+GF  F +  ++P  +
Sbjct: 630 N--NTHQASYKPLLKQVVEEIFNPEKSDPIDIEHMSSGLTDLLKTGFSMF-MKVSRPHPS 672

Query: 806 DQNVILVFVIGGINGLEVREAQEALSESGRPDIELIVGGTTFLTPHDMFDLL 850
           D  ++++FV+GG+   E +  ++ L  S +P  +++V  T  L P ++ +LL
Sbjct: 690 DHPLLILFVVGGVTVAEAKMVKD-LVASLKPGTQVMVLSTRLLKPLNIPELL 672

BLAST of Chy12G209840.1 vs. ExPASy TrEMBL
Match: A0A0A0LYK3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G538250 PE=3 SV=1)

HSP 1 Score: 1649.0 bits (4269), Expect = 0.0e+00
Identity = 836/856 (97.66%), Postives = 844/856 (98.60%), Query Frame = 0

Query: 1   MALLDVTRSCLDSISQIADHLKGSILYLDAGCVESFQILGGFPLLLDHGVNVVCSLENMA 60
           MALLDVTRSCLDSI+QIADHLKGSILYLDAGCVESFQILGGFPLLLDHGVNVVCSLENMA
Sbjct: 1   MALLDVTRSCLDSITQIADHLKGSILYLDAGCVESFQILGGFPLLLDHGVNVVCSLENMA 60

Query: 61  SLDAVIDWNPASATKLVVITSRLLSDAHRYILRCLTTHQDVRHCTIFTSISEIAQSVYPD 120
           +LDAVIDWNPASATKLVVITSRLLSDAHRYILRCLTTHQDVRHCTIFTSISEIA SVYPD
Sbjct: 61  ALDAVIDWNPASATKLVVITSRLLSDAHRYILRCLTTHQDVRHCTIFTSISEIAHSVYPD 120

Query: 121 SPLGPDAFHEYESLLVQDYEELVKKDEKRALPSEDKNLEKCISSEDEGWSRLTSSEEDIT 180
           SPLGPDAFHEYESLLVQDYEELVKKDEK+ALPSED+ LEKCISSEDEGWSRLTSSEEDIT
Sbjct: 121 SPLGPDAFHEYESLLVQDYEELVKKDEKKALPSEDRILEKCISSEDEGWSRLTSSEEDIT 180

Query: 181 QVEASSSGRDSYEDVLTSHRQDVGKKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS 240
           Q+EASSSGRDSYEDVLTSHR+DVG+KLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS
Sbjct: 181 QLEASSSGRDSYEDVLTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS 240

Query: 241 AENVDSLSPGLPPLYTGMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDLSKTVG 300
            ENVDSLSPGLPPLYTGMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDLSKTVG
Sbjct: 241 PENVDSLSPGLPPLYTGMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDLSKTVG 300

Query: 301 KILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRTSPVTHV 360
           KILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRTSPVTHV
Sbjct: 301 KILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRTSPVTHV 360

Query: 361 KGPETSLKKGPRICRRAPLDVRIPFAEILTEDEGKADKFRLGERIEAFLSGWNSGNSTSQ 420
           KGPETSLKKGPRICRRAPLDVRIPFAEILTEDEGKADKFRLGERIEAFLSGWNS NSTSQ
Sbjct: 361 KGPETSLKKGPRICRRAPLDVRIPFAEILTEDEGKADKFRLGERIEAFLSGWNSRNSTSQ 420

Query: 421 NFNKGGESNRDQTLQSPIYDPELLSGCFVSSENFRGTPYMEAILDRKTKDGTVLIKKWLQ 480
           NFN  GESNRDQTLQSPIYDPELLSGCFVSSENFRG PYMEAILDRKTKDGTVLIKKWLQ
Sbjct: 421 NFNNSGESNRDQTLQSPIYDPELLSGCFVSSENFRGIPYMEAILDRKTKDGTVLIKKWLQ 480

Query: 481 ETMRKENVAVNGKIRPGFPTKLELESMIKALAKSQTCLLRNKGVLQLAAAATVAIEELNS 540
           ETMRKENV VNGKIRPGFPTK+ELESMIKALAKSQTC LRNKGVLQLAAAATVAIEELNS
Sbjct: 481 ETMRKENVVVNGKIRPGFPTKVELESMIKALAKSQTCWLRNKGVLQLAAAATVAIEELNS 540

Query: 541 TRWDAFLSAEKILRASAEDTSQGLAAQIVDLINKSVLVVKSEASKGVLSFEDALLLTITG 600
           TRWDAFLSAEKILRASAEDTSQGLAAQIVDLINKSVLVVKSEASKGVLSFEDALLLTITG
Sbjct: 541 TRWDAFLSAEKILRASAEDTSQGLAAQIVDLINKSVLVVKSEASKGVLSFEDALLLTITG 600

Query: 601 YILAGENFPTSGSDGPFSWQEEHFIKEAIIDAILENPVDGKLKFLHGLIEELQTNRDRMI 660
           YILAGENFPTSGSDGPFSWQEEHFIKEAIIDAILENPVDGKLKFLHGLIEELQTNRDRM 
Sbjct: 601 YILAGENFPTSGSDGPFSWQEEHFIKEAIIDAILENPVDGKLKFLHGLIEELQTNRDRMK 660

Query: 661 SKGTKEMGSSQIKDDDFDDQWESWGDDDTDINTTNEEVYDDMQLKLELRDRVDSLFKTLH 720
           SKGTKEMGSSQIKDDDFDDQWESWGDDD DINTTNEEVYDDMQLKLELRDRVDSLFKTLH
Sbjct: 661 SKGTKEMGSSQIKDDDFDDQWESWGDDDADINTTNEEVYDDMQLKLELRDRVDSLFKTLH 720

Query: 721 KLSGTKKINLLLKETLNSENILNGDQYANKGVLYKLLARILNKHDLPNLEYHSSTMGRLF 780
           KLSGTKKINLLLKETLNSENILNGDQYANKGVLYKLL RILNKHDLPNLEYHSSTMGRLF
Sbjct: 721 KLSGTKKINLLLKETLNSENILNGDQYANKGVLYKLLTRILNKHDLPNLEYHSSTMGRLF 780

Query: 781 KSGFGRFGLGQAKPSLADQNVILVFVIGGINGLEVREAQEALSESGRPDIELIVGGTTFL 840
           KSGFGRFGLGQAKPSLADQNVILVFVIGGINGLEVREAQEALSESGRPDIELIVGGTTFL
Sbjct: 781 KSGFGRFGLGQAKPSLADQNVILVFVIGGINGLEVREAQEALSESGRPDIELIVGGTTFL 840

Query: 841 TPHDMFDLLLGDSAYV 857
           TPHDMFDLLLGDSAYV
Sbjct: 841 TPHDMFDLLLGDSAYV 856

BLAST of Chy12G209840.1 vs. ExPASy TrEMBL
Match: A0A5A7T609 (Sec1 family domain-containing protein MIP3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold178G00550 PE=3 SV=1)

HSP 1 Score: 1623.2 bits (4202), Expect = 0.0e+00
Identity = 819/856 (95.68%), Postives = 841/856 (98.25%), Query Frame = 0

Query: 1   MALLDVTRSCLDSISQIADHLKGSILYLDAGCVESFQILGGFPLLLDHGVNVVCSLENMA 60
           MALLDVTRSCLDSISQIADHLKGSILYLDAGCVESFQILGGFPLLLDHGVNVVCSLENMA
Sbjct: 1   MALLDVTRSCLDSISQIADHLKGSILYLDAGCVESFQILGGFPLLLDHGVNVVCSLENMA 60

Query: 61  SLDAVIDWNPASATKLVVITSRLLSDAHRYILRCLTTHQDVRHCTIFTSISEIAQSVYPD 120
           SLDAVIDWNPASATKLVVITSRLLSDAHRYILRCLTTHQ VRHCTIFTSISE+A S YPD
Sbjct: 61  SLDAVIDWNPASATKLVVITSRLLSDAHRYILRCLTTHQGVRHCTIFTSISELAHSAYPD 120

Query: 121 SPLGPDAFHEYESLLVQDYEELVKKDEKRALPSEDKNLEKCISSEDEGWSRLTSSEEDIT 180
           SPLGPDAFHEYESLLVQDYEELVKKDEK+ALPSED+NLEK ISSEDEGWSRLTSSEEDIT
Sbjct: 121 SPLGPDAFHEYESLLVQDYEELVKKDEKKALPSEDRNLEKYISSEDEGWSRLTSSEEDIT 180

Query: 181 QVEASSSGRDSYEDVLTSHRQDVGKKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS 240
           Q+EASSSGR+SYED+LT+H++DVG+KLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS
Sbjct: 181 QLEASSSGRESYEDILTNHQEDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS 240

Query: 241 AENVDSLSPGLPPLYTGMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDLSKTVG 300
           AE+VDSLSPGLPPLYTGMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDLSKTVG
Sbjct: 241 AESVDSLSPGLPPLYTGMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDLSKTVG 300

Query: 301 KILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRTSPVTHV 360
           K+LTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRTSPVTHV
Sbjct: 301 KMLTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRTSPVTHV 360

Query: 361 KGPETSLKKGPRICRRAPLDVRIPFAEILTEDEGKADKFRLGERIEAFLSGWNSGNSTSQ 420
           K PETSLKKGPRICRRAP+DVRIPFAEILTED GKADKFRLGERIEAFLSGWNSGNS SQ
Sbjct: 361 KSPETSLKKGPRICRRAPVDVRIPFAEILTEDGGKADKFRLGERIEAFLSGWNSGNSISQ 420

Query: 421 NFNKGGESNRDQTLQSPIYDPELLSGCFVSSENFRGTPYMEAILDRKTKDGTVLIKKWLQ 480
           NFNK GESNRDQ LQSPIYDPELLSGCFVSSENFRGTPYMEAILDRKTKDGTVLIKKWLQ
Sbjct: 421 NFNKSGESNRDQALQSPIYDPELLSGCFVSSENFRGTPYMEAILDRKTKDGTVLIKKWLQ 480

Query: 481 ETMRKENVAVNGKIRPGFPTKLELESMIKALAKSQTCLLRNKGVLQLAAAATVAIEELNS 540
           ETMRKENV VNGKIRPGFPTKLELESMIKALA+SQTCLL+NKGVLQLAAAATVAIEELN+
Sbjct: 481 ETMRKENVVVNGKIRPGFPTKLELESMIKALARSQTCLLKNKGVLQLAAAATVAIEELNT 540

Query: 541 TRWDAFLSAEKILRASAEDTSQGLAAQIVDLINKSVLVVKSEASKGVLSFEDALLLTITG 600
           TRWDAFLSAEKILRASAEDTSQGLAAQIVDLINKSVLVVKSE+SKG+LSFEDALLLTITG
Sbjct: 541 TRWDAFLSAEKILRASAEDTSQGLAAQIVDLINKSVLVVKSESSKGILSFEDALLLTITG 600

Query: 601 YILAGENFPTSGSDGPFSWQEEHFIKEAIIDAILENPVDGKLKFLHGLIEELQTNRDRMI 660
           YILAGENFPTSGSDGPFSWQEEHFIKEAIIDAILENPV GKLKFLHGLIEELQTNRDR+ 
Sbjct: 601 YILAGENFPTSGSDGPFSWQEEHFIKEAIIDAILENPVGGKLKFLHGLIEELQTNRDRIK 660

Query: 661 SKGTKEMGSSQIKDDDFDDQWESWGDDDTDINTTNEEVYDDMQLKLELRDRVDSLFKTLH 720
           SKGTK +GSSQIKDDDFDDQW+SWGDDD DINTTNEEVYDDMQLKLELRDRVDSLFKTLH
Sbjct: 661 SKGTKGIGSSQIKDDDFDDQWDSWGDDDADINTTNEEVYDDMQLKLELRDRVDSLFKTLH 720

Query: 721 KLSGTKKINLLLKETLNSENILNGDQYANKGVLYKLLARILNKHDLPNLEYHSSTMGRLF 780
           KLSGTKKINLLLKETLNSENILNGDQYANKGVLYKLLARILNKHDLPNLEYHSSTMGRLF
Sbjct: 721 KLSGTKKINLLLKETLNSENILNGDQYANKGVLYKLLARILNKHDLPNLEYHSSTMGRLF 780

Query: 781 KSGFGRFGLGQAKPSLADQNVILVFVIGGINGLEVREAQEALSESGRPDIELIVGGTTFL 840
           KSGFGRFGLGQAKPSLADQNVILVFVIGG+NGLEVREAQEALSESGRPDIELIVGGTTFL
Sbjct: 781 KSGFGRFGLGQAKPSLADQNVILVFVIGGVNGLEVREAQEALSESGRPDIELIVGGTTFL 840

Query: 841 TPHDMFDLLLGDSAYV 857
           TP DMFDLLLGDSAYV
Sbjct: 841 TPDDMFDLLLGDSAYV 856

BLAST of Chy12G209840.1 vs. ExPASy TrEMBL
Match: A0A1S4DSJ8 (sec1 family domain-containing protein MIP3 OS=Cucumis melo OX=3656 GN=LOC103488296 PE=3 SV=1)

HSP 1 Score: 1575.8 bits (4079), Expect = 0.0e+00
Identity = 799/856 (93.34%), Postives = 822/856 (96.03%), Query Frame = 0

Query: 1   MALLDVTRSCLDSISQIADHLKGSILYLDAGCVESFQILGGFPLLLDHGVNVVCSLENMA 60
           MALLDVTRSCLDSISQIADHLKGSILYLDAGCVESFQILGGFPLLLDHGVNVVCSLENMA
Sbjct: 1   MALLDVTRSCLDSISQIADHLKGSILYLDAGCVESFQILGGFPLLLDHGVNVVCSLENMA 60

Query: 61  SLDAVIDWNPASATKLVVITSRLLSDAHRYILRCLTTHQDVRHCTIFTSISEIAQSVYPD 120
           SLDAVIDWNPASATKLVVITSRLLSDAHRYILRCLTTHQ VRHCTIFTSISE+A S YPD
Sbjct: 61  SLDAVIDWNPASATKLVVITSRLLSDAHRYILRCLTTHQGVRHCTIFTSISELAHSAYPD 120

Query: 121 SPLGPDAFHEYESLLVQDYEELVKKDEKRALPSEDKNLEKCISSEDEGWSRLTSSEEDIT 180
           SPLGPDAFHE                  +ALPSED+NLEK ISSEDEGWSRLTSSEEDIT
Sbjct: 121 SPLGPDAFHE------------------KALPSEDRNLEKYISSEDEGWSRLTSSEEDIT 180

Query: 181 QVEASSSGRDSYEDVLTSHRQDVGKKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS 240
           Q+EASSSGR+SYED+LT+H++DVG+KLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS
Sbjct: 181 QLEASSSGRESYEDILTNHQEDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS 240

Query: 241 AENVDSLSPGLPPLYTGMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDLSKTVG 300
           AE+VDSLSPGLPPLYTGMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDLSKTVG
Sbjct: 241 AESVDSLSPGLPPLYTGMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDLSKTVG 300

Query: 301 KILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRTSPVTHV 360
           K+LTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRTSPVTHV
Sbjct: 301 KMLTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRTSPVTHV 360

Query: 361 KGPETSLKKGPRICRRAPLDVRIPFAEILTEDEGKADKFRLGERIEAFLSGWNSGNSTSQ 420
           K PETS KKGPRICRRAPLDVRIPFAEILTED GKADKFRLGERIEAFLSGWNSGNS SQ
Sbjct: 361 KSPETSQKKGPRICRRAPLDVRIPFAEILTEDGGKADKFRLGERIEAFLSGWNSGNSISQ 420

Query: 421 NFNKGGESNRDQTLQSPIYDPELLSGCFVSSENFRGTPYMEAILDRKTKDGTVLIKKWLQ 480
           NFN+ GESNRDQ LQSPIYDPELLSGCFVSSENFRGTPYMEAILDRKTKDGTVLIKKWLQ
Sbjct: 421 NFNRSGESNRDQALQSPIYDPELLSGCFVSSENFRGTPYMEAILDRKTKDGTVLIKKWLQ 480

Query: 481 ETMRKENVAVNGKIRPGFPTKLELESMIKALAKSQTCLLRNKGVLQLAAAATVAIEELNS 540
           ETMRKENV VNGKIRPGFPTKLELESMIKALA+SQTCLL+NKGVLQLAAAATVAIEELN+
Sbjct: 481 ETMRKENVVVNGKIRPGFPTKLELESMIKALARSQTCLLKNKGVLQLAAAATVAIEELNT 540

Query: 541 TRWDAFLSAEKILRASAEDTSQGLAAQIVDLINKSVLVVKSEASKGVLSFEDALLLTITG 600
           TRWDAFLSAEKILRASAEDTSQGLAAQIVDLINKSVLVVKSE+SKG+LSFEDALLLTITG
Sbjct: 541 TRWDAFLSAEKILRASAEDTSQGLAAQIVDLINKSVLVVKSESSKGILSFEDALLLTITG 600

Query: 601 YILAGENFPTSGSDGPFSWQEEHFIKEAIIDAILENPVDGKLKFLHGLIEELQTNRDRMI 660
           Y+LAGENFPTSGSDGPFSWQEEHFIKEAIIDAILENPV GKLKFLHGLIEELQTNRDR+ 
Sbjct: 601 YMLAGENFPTSGSDGPFSWQEEHFIKEAIIDAILENPVGGKLKFLHGLIEELQTNRDRIK 660

Query: 661 SKGTKEMGSSQIKDDDFDDQWESWGDDDTDINTTNEEVYDDMQLKLELRDRVDSLFKTLH 720
           SKGTK +GSSQIKDDDFDDQW+SWGDDD DINTTNEEVYDDMQLKLELRDRVDSLFKTLH
Sbjct: 661 SKGTKGIGSSQIKDDDFDDQWDSWGDDDADINTTNEEVYDDMQLKLELRDRVDSLFKTLH 720

Query: 721 KLSGTKKINLLLKETLNSENILNGDQYANKGVLYKLLARILNKHDLPNLEYHSSTMGRLF 780
           KLSGTKKINLLLKETLNSENILNGDQYANKGVLYKLLARILNKHDLPNLEYHSSTMGRLF
Sbjct: 721 KLSGTKKINLLLKETLNSENILNGDQYANKGVLYKLLARILNKHDLPNLEYHSSTMGRLF 780

Query: 781 KSGFGRFGLGQAKPSLADQNVILVFVIGGINGLEVREAQEALSESGRPDIELIVGGTTFL 840
           KSGFGRFGLGQAKPSLADQNVILVFVIGG+NGLEVREAQEALSESGRPDIELIVGGTTFL
Sbjct: 781 KSGFGRFGLGQAKPSLADQNVILVFVIGGVNGLEVREAQEALSESGRPDIELIVGGTTFL 838

Query: 841 TPHDMFDLLLGDSAYV 857
           TP DMFDLLLGDSAYV
Sbjct: 841 TPDDMFDLLLGDSAYV 838

BLAST of Chy12G209840.1 vs. ExPASy TrEMBL
Match: A0A6J1GK80 (sec1 family domain-containing protein MIP3 OS=Cucurbita moschata OX=3662 GN=LOC111455034 PE=3 SV=1)

HSP 1 Score: 1525.4 bits (3948), Expect = 0.0e+00
Identity = 772/856 (90.19%), Postives = 806/856 (94.16%), Query Frame = 0

Query: 1   MALLDVTRSCLDSISQIADHLKGSILYLDAGCVESFQILGGFPLLLDHGVNVVCSLENMA 60
           MALLDVTRSCLDSISQI+DHL+GSILYLDAGCVESFQILGGFPLLLDHGV VVCSLENM 
Sbjct: 1   MALLDVTRSCLDSISQISDHLEGSILYLDAGCVESFQILGGFPLLLDHGVQVVCSLENMT 60

Query: 61  SLDAVIDWNPASATKLVVITSRLLSDAHRYILRCLTTHQDVRHCTIFTSISEIAQSVYPD 120
           SLDAVI WN AS  KLVV TSRLLSDAHRYILRCLT HQ VRHCTIFTSISE+A S YPD
Sbjct: 61  SLDAVIGWNLASTKKLVVFTSRLLSDAHRYILRCLTAHQGVRHCTIFTSISEVAHSAYPD 120

Query: 121 SPLGPDAFHEYESLLVQDYEELVKKDEKRALPSEDKNLEKCISSEDEGWSRLTSSEEDIT 180
           SPLGPDAFHEYESLLVQDYEELVKK EK+ + SED+N EK ISSEDEGWSRLTSSEEDIT
Sbjct: 121 SPLGPDAFHEYESLLVQDYEELVKKGEKKVVLSEDRNSEKYISSEDEGWSRLTSSEEDIT 180

Query: 181 QVEASSSGRDSYEDVLTSHRQDVGKKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS 240
            VEASSSGR+SYE +LTSHR+DVG+KLVVSVHHFPMILCPFSPRVFVLPSEGL+AEACLS
Sbjct: 181 HVEASSSGRNSYEGILTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLVAEACLS 240

Query: 241 AENVDSLSPGLPPLYTGMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDLSKTVG 300
           AE+VDSLSPGLPPLYTGMPPD DDIPPGATLTAHFLYHFAAKMDLKMEIFSIGD+SKTVG
Sbjct: 241 AESVDSLSPGLPPLYTGMPPDADDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDMSKTVG 300

Query: 301 KILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRTSPVTHV 360
           K+LTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVD MFLSLPRRKR +PVTH 
Sbjct: 301 KLLTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDSMFLSLPRRKRMAPVTHA 360

Query: 361 KGPETSLKKGPRICRRAPLDVRIPFAEILTEDEGKADKFRLGERIEAFLSGWNSGNSTSQ 420
           KGPE SLK  PRICRRAPLDVRIPFAEILTED GKADKFR+G RIEAFLSGWNSGNS+SQ
Sbjct: 361 KGPEASLKHEPRICRRAPLDVRIPFAEILTEDGGKADKFRVGARIEAFLSGWNSGNSSSQ 420

Query: 421 NFNKGGESNRDQTLQSPIYDPELLSGCFVSSENFRGTPYMEAILDRKTKDGTVLIKKWLQ 480
           NF+K GESNRDQ LQSPIYDPELLSGCFVSSENFRGTP++EAILDRK KD TVLIKKWLQ
Sbjct: 421 NFDKSGESNRDQNLQSPIYDPELLSGCFVSSENFRGTPFLEAILDRKIKDATVLIKKWLQ 480

Query: 481 ETMRKENVAVNGKIRPGFPTKLELESMIKALAKSQTCLLRNKGVLQLAAAATVAIEELNS 540
           ETMRKE+V VNGKIR GFPTKLELESMIKALAKSQ+CLLRNKGVLQLAAAATVAIEE N 
Sbjct: 481 ETMRKESVVVNGKIRAGFPTKLELESMIKALAKSQSCLLRNKGVLQLAAAATVAIEESNR 540

Query: 541 TRWDAFLSAEKILRASAEDTSQGLAAQIVDLINKSVLVVKSEASKGVLSFEDALLLTITG 600
           TRWDAF SAEK+LRASAEDT QGLAAQIVDLINKSVLV KSE+SKGVLSF+DALLLTITG
Sbjct: 541 TRWDAFRSAEKVLRASAEDTIQGLAAQIVDLINKSVLVGKSESSKGVLSFQDALLLTITG 600

Query: 601 YILAGENFPTSGSDGPFSWQEEHFIKEAIIDAILENPVDGKLKFLHGLIEELQTNRDRMI 660
           YILAGENFPTSGSDGPFSWQEEHF+KEAI DAILENPV G+LKFLHGL EELQTNRDR+ 
Sbjct: 601 YILAGENFPTSGSDGPFSWQEEHFMKEAITDAILENPVGGELKFLHGLTEELQTNRDRIK 660

Query: 661 SKGTKEMGSSQIKDDDFDDQWESWGDDDTDINTTNEEVYDDMQLKLELRDRVDSLFKTLH 720
           SKGTKE   S+IKDDDFDDQWESWGD+D D NTTNEEVYDDMQLKLELRDRVDSLFKTLH
Sbjct: 661 SKGTKETSLSEIKDDDFDDQWESWGDEDADNNTTNEEVYDDMQLKLELRDRVDSLFKTLH 720

Query: 721 KLSGTKKINLLLKETLNSENILNGDQYANKGVLYKLLARILNKHDLPNLEYHSSTMGRLF 780
           KLSGTK  NLLLKETLNSENILNGDQ+ANKGVLYKLLARILNKHDLPNLEYHSSTMGRLF
Sbjct: 721 KLSGTKTRNLLLKETLNSENILNGDQFANKGVLYKLLARILNKHDLPNLEYHSSTMGRLF 780

Query: 781 KSGFGRFGLGQAKPSLADQNVILVFVIGGINGLEVREAQEALSESGRPDIELIVGGTTFL 840
           KSGFGRFGLGQAKPSLADQNVILVFV+GGINGLEVREAQEALS+SGRPDIELIVGGTTFL
Sbjct: 781 KSGFGRFGLGQAKPSLADQNVILVFVLGGINGLEVREAQEALSDSGRPDIELIVGGTTFL 840

Query: 841 TPHDMFDLLLGDSAYV 857
           TP DMFDLLLG+SAYV
Sbjct: 841 TPDDMFDLLLGESAYV 856

BLAST of Chy12G209840.1 vs. ExPASy TrEMBL
Match: A0A6J1HV02 (sec1 family domain-containing protein MIP3 OS=Cucurbita maxima OX=3661 GN=LOC111468119 PE=3 SV=1)

HSP 1 Score: 1523.1 bits (3942), Expect = 0.0e+00
Identity = 772/856 (90.19%), Postives = 805/856 (94.04%), Query Frame = 0

Query: 1   MALLDVTRSCLDSISQIADHLKGSILYLDAGCVESFQILGGFPLLLDHGVNVVCSLENMA 60
           MALLDVTRSCLDSISQI+DHL+GSILYLDAGCVESFQILGG PLLLDHGV VVCSLENM 
Sbjct: 1   MALLDVTRSCLDSISQISDHLEGSILYLDAGCVESFQILGGLPLLLDHGVQVVCSLENMT 60

Query: 61  SLDAVIDWNPASATKLVVITSRLLSDAHRYILRCLTTHQDVRHCTIFTSISEIAQSVYPD 120
           SLDAVI WN ASA KLVV TSRLLSDAHRYILRCLT HQ VRHCTIFTSISE+A S YPD
Sbjct: 61  SLDAVIGWNLASAKKLVVFTSRLLSDAHRYILRCLTAHQGVRHCTIFTSISEVAHSAYPD 120

Query: 121 SPLGPDAFHEYESLLVQDYEELVKKDEKRALPSEDKNLEKCISSEDEGWSRLTSSEEDIT 180
           SPLGPDAFHEYESLLVQDYEELVKK EK+ + SED+N  K ISSEDEGWSRLTSSEEDIT
Sbjct: 121 SPLGPDAFHEYESLLVQDYEELVKKGEKKVVLSEDRNSVKYISSEDEGWSRLTSSEEDIT 180

Query: 181 QVEASSSGRDSYEDVLTSHRQDVGKKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS 240
            VEASSSGR+SYE +LTSHR+DVG+KLVVSVHHFPMILCPFSPRVFVLPSEGL+AEACLS
Sbjct: 181 HVEASSSGRNSYEGILTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLVAEACLS 240

Query: 241 AENVDSLSPGLPPLYTGMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDLSKTVG 300
           AE+VDSLSPGLPPLYTGMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGD+SKTVG
Sbjct: 241 AESVDSLSPGLPPLYTGMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDMSKTVG 300

Query: 301 KILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRTSPVTHV 360
           K+LTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVD MFLSLPRRKR +PVTH 
Sbjct: 301 KLLTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDSMFLSLPRRKRMAPVTHA 360

Query: 361 KGPETSLKKGPRICRRAPLDVRIPFAEILTEDEGKADKFRLGERIEAFLSGWNSGNSTSQ 420
           KGPE SLK  PRICRRAPLDVRIPFAEILTED GKADKFRLG RIEAFLSGWNSGNS+SQ
Sbjct: 361 KGPEASLKHEPRICRRAPLDVRIPFAEILTEDGGKADKFRLGARIEAFLSGWNSGNSSSQ 420

Query: 421 NFNKGGESNRDQTLQSPIYDPELLSGCFVSSENFRGTPYMEAILDRKTKDGTVLIKKWLQ 480
           NF+K GESNRDQ LQSPIYDPELLSGCFVSSENFRGTP++EAILDRK KD TVLIKKWLQ
Sbjct: 421 NFDKSGESNRDQNLQSPIYDPELLSGCFVSSENFRGTPFLEAILDRKIKDATVLIKKWLQ 480

Query: 481 ETMRKENVAVNGKIRPGFPTKLELESMIKALAKSQTCLLRNKGVLQLAAAATVAIEELNS 540
           ETMRKE+V VNGKIR GFPTKLELESMIKALAKSQ+CLLRNKGVLQLAAAATVAIEE N 
Sbjct: 481 ETMRKESVVVNGKIRAGFPTKLELESMIKALAKSQSCLLRNKGVLQLAAAATVAIEESNR 540

Query: 541 TRWDAFLSAEKILRASAEDTSQGLAAQIVDLINKSVLVVKSEASKGVLSFEDALLLTITG 600
           TRWDAF SAEK+LRASAEDT QGLAAQIVDLINKSVLV KSE+SKGVLSF+DALLLTITG
Sbjct: 541 TRWDAFRSAEKVLRASAEDTIQGLAAQIVDLINKSVLVGKSESSKGVLSFQDALLLTITG 600

Query: 601 YILAGENFPTSGSDGPFSWQEEHFIKEAIIDAILENPVDGKLKFLHGLIEELQTNRDRMI 660
           YILAGENFPTSGSDGPFSWQEEHF+KEAI DAILENPV G+LKFLHGL EELQTNRDR+ 
Sbjct: 601 YILAGENFPTSGSDGPFSWQEEHFMKEAITDAILENPVGGELKFLHGLTEELQTNRDRIK 660

Query: 661 SKGTKEMGSSQIKDDDFDDQWESWGDDDTDINTTNEEVYDDMQLKLELRDRVDSLFKTLH 720
           SKGTK M  S+IKDDDFDDQWESWGD+D D NTTNEEVYDDMQLKLELRDRVDSLFK LH
Sbjct: 661 SKGTKAMSLSEIKDDDFDDQWESWGDEDADNNTTNEEVYDDMQLKLELRDRVDSLFKMLH 720

Query: 721 KLSGTKKINLLLKETLNSENILNGDQYANKGVLYKLLARILNKHDLPNLEYHSSTMGRLF 780
           KLSGTK  NLLLKETLNSENILNGDQ+ANKGVLYKLLARILNKHDLPNLEYHSSTMGRLF
Sbjct: 721 KLSGTKTRNLLLKETLNSENILNGDQFANKGVLYKLLARILNKHDLPNLEYHSSTMGRLF 780

Query: 781 KSGFGRFGLGQAKPSLADQNVILVFVIGGINGLEVREAQEALSESGRPDIELIVGGTTFL 840
           KSGFGRFGLGQAKPSLADQNVILVFV+GGINGLEVREAQEALS+SGRPDIELIVGGTTFL
Sbjct: 781 KSGFGRFGLGQAKPSLADQNVILVFVLGGINGLEVREAQEALSDSGRPDIELIVGGTTFL 840

Query: 841 TPHDMFDLLLGDSAYV 857
           TP DMFDLLLG+SAYV
Sbjct: 841 TPDDMFDLLLGESAYV 856

BLAST of Chy12G209840.1 vs. NCBI nr
Match: XP_004135552.1 (sec1 family domain-containing protein MIP3 [Cucumis sativus] >KGN65927.1 hypothetical protein Csa_023176 [Cucumis sativus])

HSP 1 Score: 1644 bits (4257), Expect = 0.0
Identity = 836/856 (97.66%), Postives = 844/856 (98.60%), Query Frame = 0

Query: 1   MALLDVTRSCLDSISQIADHLKGSILYLDAGCVESFQILGGFPLLLDHGVNVVCSLENMA 60
           MALLDVTRSCLDSI+QIADHLKGSILYLDAGCVESFQILGGFPLLLDHGVNVVCSLENMA
Sbjct: 1   MALLDVTRSCLDSITQIADHLKGSILYLDAGCVESFQILGGFPLLLDHGVNVVCSLENMA 60

Query: 61  SLDAVIDWNPASATKLVVITSRLLSDAHRYILRCLTTHQDVRHCTIFTSISEIAQSVYPD 120
           +LDAVIDWNPASATKLVVITSRLLSDAHRYILRCLTTHQDVRHCTIFTSISEIA SVYPD
Sbjct: 61  ALDAVIDWNPASATKLVVITSRLLSDAHRYILRCLTTHQDVRHCTIFTSISEIAHSVYPD 120

Query: 121 SPLGPDAFHEYESLLVQDYEELVKKDEKRALPSEDKNLEKCISSEDEGWSRLTSSEEDIT 180
           SPLGPDAFHEYESLLVQDYEELVKKDEK+ALPSED+ LEKCISSEDEGWSRLTSSEEDIT
Sbjct: 121 SPLGPDAFHEYESLLVQDYEELVKKDEKKALPSEDRILEKCISSEDEGWSRLTSSEEDIT 180

Query: 181 QVEASSSGRDSYEDVLTSHRQDVGKKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS 240
           Q+EASSSGRDSYEDVLTSHR+DVG+KLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS
Sbjct: 181 QLEASSSGRDSYEDVLTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS 240

Query: 241 AENVDSLSPGLPPLYTGMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDLSKTVG 300
            ENVDSLSPGLPPLYTGMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDLSKTVG
Sbjct: 241 PENVDSLSPGLPPLYTGMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDLSKTVG 300

Query: 301 KILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRTSPVTHV 360
           KILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRTSPVTHV
Sbjct: 301 KILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRTSPVTHV 360

Query: 361 KGPETSLKKGPRICRRAPLDVRIPFAEILTEDEGKADKFRLGERIEAFLSGWNSGNSTSQ 420
           KGPETSLKKGPRICRRAPLDVRIPFAEILTEDEGKADKFRLGERIEAFLSGWNS NSTSQ
Sbjct: 361 KGPETSLKKGPRICRRAPLDVRIPFAEILTEDEGKADKFRLGERIEAFLSGWNSRNSTSQ 420

Query: 421 NFNKGGESNRDQTLQSPIYDPELLSGCFVSSENFRGTPYMEAILDRKTKDGTVLIKKWLQ 480
           NFN  GESNRDQTLQSPIYDPELLSGCFVSSENFRG PYMEAILDRKTKDGTVLIKKWLQ
Sbjct: 421 NFNNSGESNRDQTLQSPIYDPELLSGCFVSSENFRGIPYMEAILDRKTKDGTVLIKKWLQ 480

Query: 481 ETMRKENVAVNGKIRPGFPTKLELESMIKALAKSQTCLLRNKGVLQLAAAATVAIEELNS 540
           ETMRKENV VNGKIRPGFPTK+ELESMIKALAKSQTC LRNKGVLQLAAAATVAIEELNS
Sbjct: 481 ETMRKENVVVNGKIRPGFPTKVELESMIKALAKSQTCWLRNKGVLQLAAAATVAIEELNS 540

Query: 541 TRWDAFLSAEKILRASAEDTSQGLAAQIVDLINKSVLVVKSEASKGVLSFEDALLLTITG 600
           TRWDAFLSAEKILRASAEDTSQGLAAQIVDLINKSVLVVKSEASKGVLSFEDALLLTITG
Sbjct: 541 TRWDAFLSAEKILRASAEDTSQGLAAQIVDLINKSVLVVKSEASKGVLSFEDALLLTITG 600

Query: 601 YILAGENFPTSGSDGPFSWQEEHFIKEAIIDAILENPVDGKLKFLHGLIEELQTNRDRMI 660
           YILAGENFPTSGSDGPFSWQEEHFIKEAIIDAILENPVDGKLKFLHGLIEELQTNRDRM 
Sbjct: 601 YILAGENFPTSGSDGPFSWQEEHFIKEAIIDAILENPVDGKLKFLHGLIEELQTNRDRMK 660

Query: 661 SKGTKEMGSSQIKDDDFDDQWESWGDDDTDINTTNEEVYDDMQLKLELRDRVDSLFKTLH 720
           SKGTKEMGSSQIKDDDFDDQWESWGDDD DINTTNEEVYDDMQLKLELRDRVDSLFKTLH
Sbjct: 661 SKGTKEMGSSQIKDDDFDDQWESWGDDDADINTTNEEVYDDMQLKLELRDRVDSLFKTLH 720

Query: 721 KLSGTKKINLLLKETLNSENILNGDQYANKGVLYKLLARILNKHDLPNLEYHSSTMGRLF 780
           KLSGTKKINLLLKETLNSENILNGDQYANKGVLYKLL RILNKHDLPNLEYHSSTMGRLF
Sbjct: 721 KLSGTKKINLLLKETLNSENILNGDQYANKGVLYKLLTRILNKHDLPNLEYHSSTMGRLF 780

Query: 781 KSGFGRFGLGQAKPSLADQNVILVFVIGGINGLEVREAQEALSESGRPDIELIVGGTTFL 840
           KSGFGRFGLGQAKPSLADQNVILVFVIGGINGLEVREAQEALSESGRPDIELIVGGTTFL
Sbjct: 781 KSGFGRFGLGQAKPSLADQNVILVFVIGGINGLEVREAQEALSESGRPDIELIVGGTTFL 840

Query: 841 TPHDMFDLLLGDSAYV 856
           TPHDMFDLLLGDSAYV
Sbjct: 841 TPHDMFDLLLGDSAYV 856

BLAST of Chy12G209840.1 vs. NCBI nr
Match: KAA0036925.1 (sec1 family domain-containing protein MIP3 [Cucumis melo var. makuwa] >TYK05547.1 sec1 family domain-containing protein MIP3 [Cucumis melo var. makuwa])

HSP 1 Score: 1618 bits (4190), Expect = 0.0
Identity = 819/856 (95.68%), Postives = 841/856 (98.25%), Query Frame = 0

Query: 1   MALLDVTRSCLDSISQIADHLKGSILYLDAGCVESFQILGGFPLLLDHGVNVVCSLENMA 60
           MALLDVTRSCLDSISQIADHLKGSILYLDAGCVESFQILGGFPLLLDHGVNVVCSLENMA
Sbjct: 1   MALLDVTRSCLDSISQIADHLKGSILYLDAGCVESFQILGGFPLLLDHGVNVVCSLENMA 60

Query: 61  SLDAVIDWNPASATKLVVITSRLLSDAHRYILRCLTTHQDVRHCTIFTSISEIAQSVYPD 120
           SLDAVIDWNPASATKLVVITSRLLSDAHRYILRCLTTHQ VRHCTIFTSISE+A S YPD
Sbjct: 61  SLDAVIDWNPASATKLVVITSRLLSDAHRYILRCLTTHQGVRHCTIFTSISELAHSAYPD 120

Query: 121 SPLGPDAFHEYESLLVQDYEELVKKDEKRALPSEDKNLEKCISSEDEGWSRLTSSEEDIT 180
           SPLGPDAFHEYESLLVQDYEELVKKDEK+ALPSED+NLEK ISSEDEGWSRLTSSEEDIT
Sbjct: 121 SPLGPDAFHEYESLLVQDYEELVKKDEKKALPSEDRNLEKYISSEDEGWSRLTSSEEDIT 180

Query: 181 QVEASSSGRDSYEDVLTSHRQDVGKKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS 240
           Q+EASSSGR+SYED+LT+H++DVG+KLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS
Sbjct: 181 QLEASSSGRESYEDILTNHQEDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS 240

Query: 241 AENVDSLSPGLPPLYTGMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDLSKTVG 300
           AE+VDSLSPGLPPLYTGMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDLSKTVG
Sbjct: 241 AESVDSLSPGLPPLYTGMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDLSKTVG 300

Query: 301 KILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRTSPVTHV 360
           K+LTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRTSPVTHV
Sbjct: 301 KMLTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRTSPVTHV 360

Query: 361 KGPETSLKKGPRICRRAPLDVRIPFAEILTEDEGKADKFRLGERIEAFLSGWNSGNSTSQ 420
           K PETSLKKGPRICRRAP+DVRIPFAEILTED GKADKFRLGERIEAFLSGWNSGNS SQ
Sbjct: 361 KSPETSLKKGPRICRRAPVDVRIPFAEILTEDGGKADKFRLGERIEAFLSGWNSGNSISQ 420

Query: 421 NFNKGGESNRDQTLQSPIYDPELLSGCFVSSENFRGTPYMEAILDRKTKDGTVLIKKWLQ 480
           NFNK GESNRDQ LQSPIYDPELLSGCFVSSENFRGTPYMEAILDRKTKDGTVLIKKWLQ
Sbjct: 421 NFNKSGESNRDQALQSPIYDPELLSGCFVSSENFRGTPYMEAILDRKTKDGTVLIKKWLQ 480

Query: 481 ETMRKENVAVNGKIRPGFPTKLELESMIKALAKSQTCLLRNKGVLQLAAAATVAIEELNS 540
           ETMRKENV VNGKIRPGFPTKLELESMIKALA+SQTCLL+NKGVLQLAAAATVAIEELN+
Sbjct: 481 ETMRKENVVVNGKIRPGFPTKLELESMIKALARSQTCLLKNKGVLQLAAAATVAIEELNT 540

Query: 541 TRWDAFLSAEKILRASAEDTSQGLAAQIVDLINKSVLVVKSEASKGVLSFEDALLLTITG 600
           TRWDAFLSAEKILRASAEDTSQGLAAQIVDLINKSVLVVKSE+SKG+LSFEDALLLTITG
Sbjct: 541 TRWDAFLSAEKILRASAEDTSQGLAAQIVDLINKSVLVVKSESSKGILSFEDALLLTITG 600

Query: 601 YILAGENFPTSGSDGPFSWQEEHFIKEAIIDAILENPVDGKLKFLHGLIEELQTNRDRMI 660
           YILAGENFPTSGSDGPFSWQEEHFIKEAIIDAILENPV GKLKFLHGLIEELQTNRDR+ 
Sbjct: 601 YILAGENFPTSGSDGPFSWQEEHFIKEAIIDAILENPVGGKLKFLHGLIEELQTNRDRIK 660

Query: 661 SKGTKEMGSSQIKDDDFDDQWESWGDDDTDINTTNEEVYDDMQLKLELRDRVDSLFKTLH 720
           SKGTK +GSSQIKDDDFDDQW+SWGDDD DINTTNEEVYDDMQLKLELRDRVDSLFKTLH
Sbjct: 661 SKGTKGIGSSQIKDDDFDDQWDSWGDDDADINTTNEEVYDDMQLKLELRDRVDSLFKTLH 720

Query: 721 KLSGTKKINLLLKETLNSENILNGDQYANKGVLYKLLARILNKHDLPNLEYHSSTMGRLF 780
           KLSGTKKINLLLKETLNSENILNGDQYANKGVLYKLLARILNKHDLPNLEYHSSTMGRLF
Sbjct: 721 KLSGTKKINLLLKETLNSENILNGDQYANKGVLYKLLARILNKHDLPNLEYHSSTMGRLF 780

Query: 781 KSGFGRFGLGQAKPSLADQNVILVFVIGGINGLEVREAQEALSESGRPDIELIVGGTTFL 840
           KSGFGRFGLGQAKPSLADQNVILVFVIGG+NGLEVREAQEALSESGRPDIELIVGGTTFL
Sbjct: 781 KSGFGRFGLGQAKPSLADQNVILVFVIGGVNGLEVREAQEALSESGRPDIELIVGGTTFL 840

Query: 841 TPHDMFDLLLGDSAYV 856
           TP DMFDLLLGDSAYV
Sbjct: 841 TPDDMFDLLLGDSAYV 856

BLAST of Chy12G209840.1 vs. NCBI nr
Match: XP_016898959.1 (PREDICTED: sec1 family domain-containing protein MIP3 [Cucumis melo])

HSP 1 Score: 1572 bits (4070), Expect = 0.0
Identity = 799/856 (93.34%), Postives = 822/856 (96.03%), Query Frame = 0

Query: 1   MALLDVTRSCLDSISQIADHLKGSILYLDAGCVESFQILGGFPLLLDHGVNVVCSLENMA 60
           MALLDVTRSCLDSISQIADHLKGSILYLDAGCVESFQILGGFPLLLDHGVNVVCSLENMA
Sbjct: 1   MALLDVTRSCLDSISQIADHLKGSILYLDAGCVESFQILGGFPLLLDHGVNVVCSLENMA 60

Query: 61  SLDAVIDWNPASATKLVVITSRLLSDAHRYILRCLTTHQDVRHCTIFTSISEIAQSVYPD 120
           SLDAVIDWNPASATKLVVITSRLLSDAHRYILRCLTTHQ VRHCTIFTSISE+A S YPD
Sbjct: 61  SLDAVIDWNPASATKLVVITSRLLSDAHRYILRCLTTHQGVRHCTIFTSISELAHSAYPD 120

Query: 121 SPLGPDAFHEYESLLVQDYEELVKKDEKRALPSEDKNLEKCISSEDEGWSRLTSSEEDIT 180
           SPLGPDAFHE                  +ALPSED+NLEK ISSEDEGWSRLTSSEEDIT
Sbjct: 121 SPLGPDAFHE------------------KALPSEDRNLEKYISSEDEGWSRLTSSEEDIT 180

Query: 181 QVEASSSGRDSYEDVLTSHRQDVGKKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS 240
           Q+EASSSGR+SYED+LT+H++DVG+KLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS
Sbjct: 181 QLEASSSGRESYEDILTNHQEDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS 240

Query: 241 AENVDSLSPGLPPLYTGMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDLSKTVG 300
           AE+VDSLSPGLPPLYTGMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDLSKTVG
Sbjct: 241 AESVDSLSPGLPPLYTGMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDLSKTVG 300

Query: 301 KILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRTSPVTHV 360
           K+LTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRTSPVTHV
Sbjct: 301 KMLTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRTSPVTHV 360

Query: 361 KGPETSLKKGPRICRRAPLDVRIPFAEILTEDEGKADKFRLGERIEAFLSGWNSGNSTSQ 420
           K PETS KKGPRICRRAPLDVRIPFAEILTED GKADKFRLGERIEAFLSGWNSGNS SQ
Sbjct: 361 KSPETSQKKGPRICRRAPLDVRIPFAEILTEDGGKADKFRLGERIEAFLSGWNSGNSISQ 420

Query: 421 NFNKGGESNRDQTLQSPIYDPELLSGCFVSSENFRGTPYMEAILDRKTKDGTVLIKKWLQ 480
           NFN+ GESNRDQ LQSPIYDPELLSGCFVSSENFRGTPYMEAILDRKTKDGTVLIKKWLQ
Sbjct: 421 NFNRSGESNRDQALQSPIYDPELLSGCFVSSENFRGTPYMEAILDRKTKDGTVLIKKWLQ 480

Query: 481 ETMRKENVAVNGKIRPGFPTKLELESMIKALAKSQTCLLRNKGVLQLAAAATVAIEELNS 540
           ETMRKENV VNGKIRPGFPTKLELESMIKALA+SQTCLL+NKGVLQLAAAATVAIEELN+
Sbjct: 481 ETMRKENVVVNGKIRPGFPTKLELESMIKALARSQTCLLKNKGVLQLAAAATVAIEELNT 540

Query: 541 TRWDAFLSAEKILRASAEDTSQGLAAQIVDLINKSVLVVKSEASKGVLSFEDALLLTITG 600
           TRWDAFLSAEKILRASAEDTSQGLAAQIVDLINKSVLVVKSE+SKG+LSFEDALLLTITG
Sbjct: 541 TRWDAFLSAEKILRASAEDTSQGLAAQIVDLINKSVLVVKSESSKGILSFEDALLLTITG 600

Query: 601 YILAGENFPTSGSDGPFSWQEEHFIKEAIIDAILENPVDGKLKFLHGLIEELQTNRDRMI 660
           Y+LAGENFPTSGSDGPFSWQEEHFIKEAIIDAILENPV GKLKFLHGLIEELQTNRDR+ 
Sbjct: 601 YMLAGENFPTSGSDGPFSWQEEHFIKEAIIDAILENPVGGKLKFLHGLIEELQTNRDRIK 660

Query: 661 SKGTKEMGSSQIKDDDFDDQWESWGDDDTDINTTNEEVYDDMQLKLELRDRVDSLFKTLH 720
           SKGTK +GSSQIKDDDFDDQW+SWGDDD DINTTNEEVYDDMQLKLELRDRVDSLFKTLH
Sbjct: 661 SKGTKGIGSSQIKDDDFDDQWDSWGDDDADINTTNEEVYDDMQLKLELRDRVDSLFKTLH 720

Query: 721 KLSGTKKINLLLKETLNSENILNGDQYANKGVLYKLLARILNKHDLPNLEYHSSTMGRLF 780
           KLSGTKKINLLLKETLNSENILNGDQYANKGVLYKLLARILNKHDLPNLEYHSSTMGRLF
Sbjct: 721 KLSGTKKINLLLKETLNSENILNGDQYANKGVLYKLLARILNKHDLPNLEYHSSTMGRLF 780

Query: 781 KSGFGRFGLGQAKPSLADQNVILVFVIGGINGLEVREAQEALSESGRPDIELIVGGTTFL 840
           KSGFGRFGLGQAKPSLADQNVILVFVIGG+NGLEVREAQEALSESGRPDIELIVGGTTFL
Sbjct: 781 KSGFGRFGLGQAKPSLADQNVILVFVIGGVNGLEVREAQEALSESGRPDIELIVGGTTFL 838

Query: 841 TPHDMFDLLLGDSAYV 856
           TP DMFDLLLGDSAYV
Sbjct: 841 TPDDMFDLLLGDSAYV 838

BLAST of Chy12G209840.1 vs. NCBI nr
Match: XP_038888123.1 (sec1 family domain-containing protein MIP3 [Benincasa hispida] >XP_038888124.1 sec1 family domain-containing protein MIP3 [Benincasa hispida])

HSP 1 Score: 1571 bits (4067), Expect = 0.0
Identity = 796/856 (92.99%), Postives = 826/856 (96.50%), Query Frame = 0

Query: 1   MALLDVTRSCLDSISQIADHLKGSILYLDAGCVESFQILGGFPLLLDHGVNVVCSLENMA 60
           MALLDVTRSCLDSISQI+DHL+GS+LYLDAGCVESFQILGGFPLLLD GV+VVCSLENM 
Sbjct: 1   MALLDVTRSCLDSISQISDHLEGSVLYLDAGCVESFQILGGFPLLLDLGVHVVCSLENMT 60

Query: 61  SLDAVIDWNPASATKLVVITSRLLSDAHRYILRCLTTHQDVRHCTIFTSISEIAQSVYPD 120
           SLDAVIDWNPASA KLVVITSRLLSDAHRYILRCLTTHQ VRHC IFTSISEIA S YPD
Sbjct: 61  SLDAVIDWNPASAKKLVVITSRLLSDAHRYILRCLTTHQGVRHCRIFTSISEIAHSAYPD 120

Query: 121 SPLGPDAFHEYESLLVQDYEELVKKDEKRALPSEDKNLEKCISSEDEGWSRLTSSEEDIT 180
           SPLGPDAFHEYESLLVQDYEELVKK  K+A+PSED+NLEK ISSEDEGWSRLTSSEEDIT
Sbjct: 121 SPLGPDAFHEYESLLVQDYEELVKKGGKKAVPSEDRNLEKYISSEDEGWSRLTSSEEDIT 180

Query: 181 QVEASSSGRDSYEDVLTSHRQDVGKKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS 240
           Q+EAS SGRDSYED+LTSHR+DVG+KLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS
Sbjct: 181 QLEASPSGRDSYEDILTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS 240

Query: 241 AENVDSLSPGLPPLYTGMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDLSKTVG 300
           AE+VDSLSPGLPPLY GMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDLSKTVG
Sbjct: 241 AESVDSLSPGLPPLYIGMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDLSKTVG 300

Query: 301 KILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRTSPVTHV 360
           KILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRTSPV+HV
Sbjct: 301 KILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRTSPVSHV 360

Query: 361 KGPETSLKKGPRICRRAPLDVRIPFAEILTEDEGKADKFRLGERIEAFLSGWNSGNSTSQ 420
           KGPETSL+ GPRICRRAPLDVRIP AEILTED GKADKFRLGERIE FLSGWNSG+ST Q
Sbjct: 361 KGPETSLRHGPRICRRAPLDVRIPLAEILTEDGGKADKFRLGERIEVFLSGWNSGSSTPQ 420

Query: 421 NFNKGGESNRDQTLQSPIYDPELLSGCFVSSENFRGTPYMEAILDRKTKDGTVLIKKWLQ 480
           NF+KGGESNRDQ LQSPIYDPELLSGCFVSSENFRGT Y+EAILDRKTKDGTVLIKKWLQ
Sbjct: 421 NFDKGGESNRDQNLQSPIYDPELLSGCFVSSENFRGTSYLEAILDRKTKDGTVLIKKWLQ 480

Query: 481 ETMRKENVAVNGKIRPGFPTKLELESMIKALAKSQTCLLRNKGVLQLAAAATVAIEELNS 540
           ETMRKE+V VNGKIRPGFPTKLELESMIKAL+KSQTCLLRNKGVLQLAAAATVAIEELNS
Sbjct: 481 ETMRKESVVVNGKIRPGFPTKLELESMIKALSKSQTCLLRNKGVLQLAAAATVAIEELNS 540

Query: 541 TRWDAFLSAEKILRASAEDTSQGLAAQIVDLINKSVLVVKSEASKGVLSFEDALLLTITG 600
           TRWDAFLSAEKILRASAEDTSQGLAAQIVDLINKSVL VKS++SKG+LSF+DALLLTITG
Sbjct: 541 TRWDAFLSAEKILRASAEDTSQGLAAQIVDLINKSVLAVKSDSSKGILSFQDALLLTITG 600

Query: 601 YILAGENFPTSGSDGPFSWQEEHFIKEAIIDAILENPVDGKLKFLHGLIEELQTNRDRMI 660
           YILAGENFPTSGSDGPFSWQEEHF+KEAI DAILENPVDGKL FLHGLIEELQTNRDR+ 
Sbjct: 601 YILAGENFPTSGSDGPFSWQEEHFMKEAITDAILENPVDGKLNFLHGLIEELQTNRDRIK 660

Query: 661 SKGTKEMGSSQIKDDDFDDQWESWGDDDTDINTTNEEVYDDMQLKLELRDRVDSLFKTLH 720
            KGTK MGS +IKDDDFDDQWESWGD+D DINTT+EEVYDDMQLKLELRDRVD+LFKTLH
Sbjct: 661 LKGTKVMGSMEIKDDDFDDQWESWGDEDADINTTSEEVYDDMQLKLELRDRVDNLFKTLH 720

Query: 721 KLSGTKKINLLLKETLNSENILNGDQYANKGVLYKLLARILNKHDLPNLEYHSSTMGRLF 780
           KLSGTKK NLLLKETLNSENILNGDQYANKGVLYKLLARILNKHDLPNLEYHSSTMGRLF
Sbjct: 721 KLSGTKKRNLLLKETLNSENILNGDQYANKGVLYKLLARILNKHDLPNLEYHSSTMGRLF 780

Query: 781 KSGFGRFGLGQAKPSLADQNVILVFVIGGINGLEVREAQEALSESGRPDIELIVGGTTFL 840
           KSGFGRFGLGQAKP+LADQNVILVFVIGGINGLEVREAQEALSESGRPDIELIVGGTTFL
Sbjct: 781 KSGFGRFGLGQAKPTLADQNVILVFVIGGINGLEVREAQEALSESGRPDIELIVGGTTFL 840

Query: 841 TPHDMFDLLLGDSAYV 856
           TP DMFDLLLGDSAYV
Sbjct: 841 TPDDMFDLLLGDSAYV 856

BLAST of Chy12G209840.1 vs. NCBI nr
Match: XP_022952322.1 (sec1 family domain-containing protein MIP3 [Cucurbita moschata] >XP_022952323.1 sec1 family domain-containing protein MIP3 [Cucurbita moschata] >XP_022952324.1 sec1 family domain-containing protein MIP3 [Cucurbita moschata])

HSP 1 Score: 1520 bits (3936), Expect = 0.0
Identity = 772/856 (90.19%), Postives = 806/856 (94.16%), Query Frame = 0

Query: 1   MALLDVTRSCLDSISQIADHLKGSILYLDAGCVESFQILGGFPLLLDHGVNVVCSLENMA 60
           MALLDVTRSCLDSISQI+DHL+GSILYLDAGCVESFQILGGFPLLLDHGV VVCSLENM 
Sbjct: 1   MALLDVTRSCLDSISQISDHLEGSILYLDAGCVESFQILGGFPLLLDHGVQVVCSLENMT 60

Query: 61  SLDAVIDWNPASATKLVVITSRLLSDAHRYILRCLTTHQDVRHCTIFTSISEIAQSVYPD 120
           SLDAVI WN AS  KLVV TSRLLSDAHRYILRCLT HQ VRHCTIFTSISE+A S YPD
Sbjct: 61  SLDAVIGWNLASTKKLVVFTSRLLSDAHRYILRCLTAHQGVRHCTIFTSISEVAHSAYPD 120

Query: 121 SPLGPDAFHEYESLLVQDYEELVKKDEKRALPSEDKNLEKCISSEDEGWSRLTSSEEDIT 180
           SPLGPDAFHEYESLLVQDYEELVKK EK+ + SED+N EK ISSEDEGWSRLTSSEEDIT
Sbjct: 121 SPLGPDAFHEYESLLVQDYEELVKKGEKKVVLSEDRNSEKYISSEDEGWSRLTSSEEDIT 180

Query: 181 QVEASSSGRDSYEDVLTSHRQDVGKKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS 240
            VEASSSGR+SYE +LTSHR+DVG+KLVVSVHHFPMILCPFSPRVFVLPSEGL+AEACLS
Sbjct: 181 HVEASSSGRNSYEGILTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLVAEACLS 240

Query: 241 AENVDSLSPGLPPLYTGMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDLSKTVG 300
           AE+VDSLSPGLPPLYTGMPPD DDIPPGATLTAHFLYHFAAKMDLKMEIFSIGD+SKTVG
Sbjct: 241 AESVDSLSPGLPPLYTGMPPDADDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDMSKTVG 300

Query: 301 KILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRTSPVTHV 360
           K+LTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVD MFLSLPRRKR +PVTH 
Sbjct: 301 KLLTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDSMFLSLPRRKRMAPVTHA 360

Query: 361 KGPETSLKKGPRICRRAPLDVRIPFAEILTEDEGKADKFRLGERIEAFLSGWNSGNSTSQ 420
           KGPE SLK  PRICRRAPLDVRIPFAEILTED GKADKFR+G RIEAFLSGWNSGNS+SQ
Sbjct: 361 KGPEASLKHEPRICRRAPLDVRIPFAEILTEDGGKADKFRVGARIEAFLSGWNSGNSSSQ 420

Query: 421 NFNKGGESNRDQTLQSPIYDPELLSGCFVSSENFRGTPYMEAILDRKTKDGTVLIKKWLQ 480
           NF+K GESNRDQ LQSPIYDPELLSGCFVSSENFRGTP++EAILDRK KD TVLIKKWLQ
Sbjct: 421 NFDKSGESNRDQNLQSPIYDPELLSGCFVSSENFRGTPFLEAILDRKIKDATVLIKKWLQ 480

Query: 481 ETMRKENVAVNGKIRPGFPTKLELESMIKALAKSQTCLLRNKGVLQLAAAATVAIEELNS 540
           ETMRKE+V VNGKIR GFPTKLELESMIKALAKSQ+CLLRNKGVLQLAAAATVAIEE N 
Sbjct: 481 ETMRKESVVVNGKIRAGFPTKLELESMIKALAKSQSCLLRNKGVLQLAAAATVAIEESNR 540

Query: 541 TRWDAFLSAEKILRASAEDTSQGLAAQIVDLINKSVLVVKSEASKGVLSFEDALLLTITG 600
           TRWDAF SAEK+LRASAEDT QGLAAQIVDLINKSVLV KSE+SKGVLSF+DALLLTITG
Sbjct: 541 TRWDAFRSAEKVLRASAEDTIQGLAAQIVDLINKSVLVGKSESSKGVLSFQDALLLTITG 600

Query: 601 YILAGENFPTSGSDGPFSWQEEHFIKEAIIDAILENPVDGKLKFLHGLIEELQTNRDRMI 660
           YILAGENFPTSGSDGPFSWQEEHF+KEAI DAILENPV G+LKFLHGL EELQTNRDR+ 
Sbjct: 601 YILAGENFPTSGSDGPFSWQEEHFMKEAITDAILENPVGGELKFLHGLTEELQTNRDRIK 660

Query: 661 SKGTKEMGSSQIKDDDFDDQWESWGDDDTDINTTNEEVYDDMQLKLELRDRVDSLFKTLH 720
           SKGTKE   S+IKDDDFDDQWESWGD+D D NTTNEEVYDDMQLKLELRDRVDSLFKTLH
Sbjct: 661 SKGTKETSLSEIKDDDFDDQWESWGDEDADNNTTNEEVYDDMQLKLELRDRVDSLFKTLH 720

Query: 721 KLSGTKKINLLLKETLNSENILNGDQYANKGVLYKLLARILNKHDLPNLEYHSSTMGRLF 780
           KLSGTK  NLLLKETLNSENILNGDQ+ANKGVLYKLLARILNKHDLPNLEYHSSTMGRLF
Sbjct: 721 KLSGTKTRNLLLKETLNSENILNGDQFANKGVLYKLLARILNKHDLPNLEYHSSTMGRLF 780

Query: 781 KSGFGRFGLGQAKPSLADQNVILVFVIGGINGLEVREAQEALSESGRPDIELIVGGTTFL 840
           KSGFGRFGLGQAKPSLADQNVILVFV+GGINGLEVREAQEALS+SGRPDIELIVGGTTFL
Sbjct: 781 KSGFGRFGLGQAKPSLADQNVILVFVLGGINGLEVREAQEALSDSGRPDIELIVGGTTFL 840

Query: 841 TPHDMFDLLLGDSAYV 856
           TP DMFDLLLG+SAYV
Sbjct: 841 TPDDMFDLLLGESAYV 856

BLAST of Chy12G209840.1 vs. TAIR 10
Match: AT2G42700.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: vesicle-mediated transport, vesicle docking involved in exocytosis; LOCATED IN: cellular_component unknown; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Sec1-like protein (InterPro:IPR001619); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 950.7 bits (2456), Expect = 8.3e-277
Identity = 509/870 (58.51%), Postives = 637/870 (73.22%), Query Frame = 0

Query: 1   MALLDVTRSCLDSISQIADHLKGSILYLDAGCVESFQILGGFPLLLDHGVNVVCSLENMA 60
           MAL+DV  SCL+SI +I + +K +I+Y+DAGC ESFQ +G FPL L+ G   VCSLENM 
Sbjct: 1   MALIDVAISCLNSIREIEEDVKDAIVYIDAGCTESFQFVGAFPLFLELGARAVCSLENMT 60

Query: 61  SLDAVIDWNPAS--ATKLVVITSRLLSDAHRYILRCLTTHQDVRHCTIFTSISEIAQSVY 120
           SLDAV DWN  S  A ++V++TSRLL+DAHRY+LRCL+TH+ V+ CT+FTSISE + S  
Sbjct: 61  SLDAVADWNSKSDCAKRIVIMTSRLLNDAHRYMLRCLSTHEGVQRCTVFTSISEGSHSAI 120

Query: 121 PDSPLGPDAFHEYESLLVQDYEELVKKDEKRALPSEDKNLEKCISSEDEGWSRLTSSEED 180
           PDSPLGPDA+ EYE+LLVQDY E  KK +K               S+D+G S+ +S+ E 
Sbjct: 121 PDSPLGPDAYREYETLLVQDYNEHTKKSDK--------------ISKDKGVSKFSSALES 180

Query: 181 ITQVEASSSGRDSYEDVLTSHRQDVGKKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEAC 240
           +T     S       D+ +   Q     LVVSVHHFP+I+CPF+PR FVLPS+G +AEA 
Sbjct: 181 LTMEPIESEN----VDISSGGAQG----LVVSVHHFPLIICPFTPRAFVLPSQGSVAEAS 240

Query: 241 LSAENVDSLSPGLPPLYTGMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDLSKT 300
           LS ++ DSLS GLPP+ TG   D DD+PPGATLTAHFLY  A KM+LK+EIFS+GD SK 
Sbjct: 241 LSRQHEDSLSFGLPPISTGSMSDTDDVPPGATLTAHFLYQLALKMELKLEIFSLGDQSKN 300

Query: 301 VGKILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRTSPVT 360
           VGKILTDMSS+YDV RRK+SAGLLLVDRTLDL+TPCCHGDSL DR+F SLPR +R S   
Sbjct: 301 VGKILTDMSSVYDVARRKRSAGLLLVDRTLDLITPCCHGDSLFDRIFSSLPRAERFS--- 360

Query: 361 HVKGPETSLKKGPRICRRAPLDVRIPFAEILTEDEGKADKFRLGERIEAFLSGWNSGNST 420
                +  LK+G     R  LDV++P  E+L E+  K     L E IEAFL GW+S  S 
Sbjct: 361 ----SQAQLKQGVPSINRPSLDVQVPLGELLNEEPSKIRDSGLPEGIEAFLRGWDSYTSA 420

Query: 421 SQNFNKGGESNRDQTLQSPIYDPELLSGCFVSSENFRGTPYMEAILDRKTKDGTVLIKKW 480
            QN     E ++  T        ELL+G  V++E FRGTPY+EA++DRKTKDG+VL+KKW
Sbjct: 421 PQNVGLFNECDKKSTTNW----TELLNGSLVATECFRGTPYLEAMIDRKTKDGSVLVKKW 480

Query: 481 LQETMRKENVAVNGKIRPGFPTKLELESMIKALAKSQTCLLRNKGVLQLAAAATVAIEEL 540
           LQE +R+EN++VN + RPG+ TK EL++MIKAL++SQ+ LL+NKG++QL AA   A++E 
Sbjct: 481 LQEALRRENISVNVRARPGYATKPELQAMIKALSQSQSSLLKNKGIIQLGAATAAALDES 540

Query: 541 NSTRWDAFLSAEKILRASAEDTSQGLAAQIVDLINKSVL----VVKSE----ASKGVLSF 600
            S +WD F SAE +L  SA DTSQGLAAQI DLINKS +      K+E    +S+G+LSF
Sbjct: 541 QSAKWDTFSSAEMMLNVSAGDTSQGLAAQISDLINKSAVAELQAKKNEKPDSSSRGLLSF 600

Query: 601 EDALLLTITGYILAGENFPTSGSDGPFSWQEEHFIKEAIIDAILENPVDGKLKFLHGLIE 660
            DALLLTI GYILAGENFPTSGS GPFSWQEEHF+KEAI+DA+LENP  G LKFL+GL E
Sbjct: 601 RDALLLTIVGYILAGENFPTSGSGGPFSWQEEHFLKEAIVDAVLENPSAGNLKFLNGLTE 660

Query: 661 ELQTNRDRMISKGTKEMGSSQIKDDDF--DDQWESWGDDDTD--INTTNEEVYDDMQLKL 720
           EL+   +R+ S+ TKE+ S    D D   DD W  WGD++ +   N+  +E YDDMQLKL
Sbjct: 661 ELEGRLNRLKSEETKEIPSDDQLDIDALDDDPWGKWGDEEEEEVDNSKADESYDDMQLKL 720

Query: 721 ELRDRVDSLFKTLHKLSGTKKINLLLKE-TLNSENILNGDQYANKGVLYKLLARILNKHD 780
           +LRDRVDSLF+ LHKLS  +  NL L+E +L SE+   G+   NKG++Y+L+ ++L+K +
Sbjct: 721 DLRDRVDSLFRFLHKLSSLRTRNLPLREGSLASESSFPGEPSGNKGLVYRLITKVLSKQE 780

Query: 781 LPNLEYHSSTMGRLFKSGFGRFGLGQAKPSLADQNVILVFVIGGINGLEVREAQEALSES 840
           +P LEYHSST+GR  KSGFGRFGLGQAKPSLADQ+VILVFVIGGING+EV EAQEA+SES
Sbjct: 781 IPGLEYHSSTVGRFIKSGFGRFGLGQAKPSLADQSVILVFVIGGINGIEVLEAQEAVSES 837

Query: 841 GRPDIELIVGGTTFLTPHDMFDLLLGDSAY 856
           GRPDI L++GGTT LTP DMF+LLLG  ++
Sbjct: 841 GRPDINLVIGGTTLLTPDDMFELLLGQFSH 837

BLAST of Chy12G209840.1 vs. TAIR 10
Match: AT2G42700.2 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: vesicle-mediated transport, vesicle docking involved in exocytosis; LOCATED IN: cellular_component unknown; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Sec1-like protein (InterPro:IPR001619). )

HSP 1 Score: 935.6 bits (2417), Expect = 2.8e-272
Identity = 509/899 (56.62%), Postives = 637/899 (70.86%), Query Frame = 0

Query: 1   MALLDVTRSCLDSISQIADHLKGSILYLDAGCVESFQILGGFPLLLDHGVNVVCSLENMA 60
           MAL+DV  SCL+SI +I + +K +I+Y+DAGC ESFQ +G FPL L+ G   VCSLENM 
Sbjct: 1   MALIDVAISCLNSIREIEEDVKDAIVYIDAGCTESFQFVGAFPLFLELGARAVCSLENMT 60

Query: 61  SLDAVIDWNPAS--ATKLVVITSRLLSDAHRYILRCLTTHQDVRHCTIFTSISEIAQSVY 120
           SLDAV DWN  S  A ++V++TSRLL+DAHRY+LRCL+TH+ V+ CT+FTSISE + S  
Sbjct: 61  SLDAVADWNSKSDCAKRIVIMTSRLLNDAHRYMLRCLSTHEGVQRCTVFTSISEGSHSAI 120

Query: 121 PDSPLGPDAFHEYESLLVQDYEELVKKDEKRALPSEDKNLEKCISSEDEGWSRLTSSEED 180
           PDSPLGPDA+ EYE+LLVQDY E  KK +K               S+D+G S+ +S+ E 
Sbjct: 121 PDSPLGPDAYREYETLLVQDYNEHTKKSDK--------------ISKDKGVSKFSSALES 180

Query: 181 ITQVEASSSGRDSYEDVLTSHRQDVGKKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEAC 240
           +T     S       D+ +   Q     LVVSVHHFP+I+CPF+PR FVLPS+G +AEA 
Sbjct: 181 LTMEPIESEN----VDISSGGAQG----LVVSVHHFPLIICPFTPRAFVLPSQGSVAEAS 240

Query: 241 LSAENVDSLSPGLPPLYTGMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDLSKT 300
           LS ++ DSLS GLPP+ TG   D DD+PPGATLTAHFLY  A KM+LK+EIFS+GD SK 
Sbjct: 241 LSRQHEDSLSFGLPPISTGSMSDTDDVPPGATLTAHFLYQLALKMELKLEIFSLGDQSKN 300

Query: 301 VGKILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRTSPVT 360
           VGKILTDMSS+YDV RRK+SAGLLLVDRTLDL+TPCCHGDSL DR+F SLPR +R S   
Sbjct: 301 VGKILTDMSSVYDVARRKRSAGLLLVDRTLDLITPCCHGDSLFDRIFSSLPRAERFS--- 360

Query: 361 HVKGPETSLKKGPRICRRAPLDVRIPFAEILTEDEGKADKFRLGERIEAFLSGWNSGNST 420
                +  LK+G     R  LDV++P  E+L E+  K     L E IEAFL GW+S  S 
Sbjct: 361 ----SQAQLKQGVPSINRPSLDVQVPLGELLNEEPSKIRDSGLPEGIEAFLRGWDSYTSA 420

Query: 421 SQNFNKGGESNRDQTLQSPIYDPELLSGCFVSSENFRGTPYMEAILDRKTKDGTVLIKKW 480
            QN     E ++  T        ELL+G  V++E FRGTPY+EA++DRKTKDG+VL+KKW
Sbjct: 421 PQNVGLFNECDKKSTTNW----TELLNGSLVATECFRGTPYLEAMIDRKTKDGSVLVKKW 480

Query: 481 LQETMRKENVAVNGKIRPGFPTKLELESMIKALAKSQTCLLRNKGVLQLAAAATVAIEEL 540
           LQE +R+EN++VN + RPG+ TK EL++MIKAL++SQ+ LL+NKG++QL AA   A++E 
Sbjct: 481 LQEALRRENISVNVRARPGYATKPELQAMIKALSQSQSSLLKNKGIIQLGAATAAALDES 540

Query: 541 NSTRWDAFLSAEKILRASAEDTSQGLAAQIVDLINKSVL----VVKSE----ASKGVLSF 600
            S +WD F SAE +L  SA DTSQGLAAQI DLINKS +      K+E    +S+G+LSF
Sbjct: 541 QSAKWDTFSSAEMMLNVSAGDTSQGLAAQISDLINKSAVAELQAKKNEKPDSSSRGLLSF 600

Query: 601 EDALLLTITGYILAGENFPTSGSDGPFSWQEEHFIKEAIIDAILENPVDGKLKFLHGLIE 660
            DALLLTI GYILAGENFPTSGS GPFSWQEEHF+KEAI+DA+LENP  G LKFL+GL E
Sbjct: 601 RDALLLTIVGYILAGENFPTSGSGGPFSWQEEHFLKEAIVDAVLENPSAGNLKFLNGLTE 660

Query: 661 ELQTNRDRMISKGTKEMGSSQIKDDDF--DDQWESWGDDDTD--INTTNEEVYDDMQLKL 720
           EL+   +R+ S+ TKE+ S    D D   DD W  WGD++ +   N+  +E YDDMQLKL
Sbjct: 661 ELEGRLNRLKSEETKEIPSDDQLDIDALDDDPWGKWGDEEEEEVDNSKADESYDDMQLKL 720

Query: 721 ELRDRVDSLFKTLHKLSGTKKINLLLKE-TLNSENILNGDQYANKGVLYKLLARILNKHD 780
           +LRDRVDSLF+ LHKLS  +  NL L+E +L SE+   G+   NKG++Y+L+ ++L+K +
Sbjct: 721 DLRDRVDSLFRFLHKLSSLRTRNLPLREGSLASESSFPGEPSGNKGLVYRLITKVLSKQE 780

Query: 781 LPNLEYHSSTMGRLFKSGFGRFGLGQAKPSLADQNVILVFVIGGINGLEVR--------- 840
           +P LEYHSST+GR  KSGFGRFGLGQAKPSLADQ+VILVFVIGGING+EV          
Sbjct: 781 IPGLEYHSSTVGRFIKSGFGRFGLGQAKPSLADQSVILVFVIGGINGIEVSFFITKVTSH 840

Query: 841 --------------------EAQEALSESGRPDIELIVGGTTFLTPHDMFDLLLGDSAY 856
                               EAQEA+SESGRPDI L++GGTT LTP DMF+LLLG  ++
Sbjct: 841 SRCRAIFFITVYVSSIVKVLEAQEAVSESGRPDINLVIGGTTLLTPDDMFELLLGQFSH 866