Chy12G208620.1 (mRNA) Cucumber (hystrix) v1

Overview


Name	Chy12G208620.1
Type	mRNA
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	ervatamin-B-like
Location	chrH12: 2975630 .. 2977090 (+)
Sequence length	1116
RNA-Seq Expression	Chy12G208620.1
Synteny	Chy12G208620.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AATTCATCCTATTATTCAAATCTTTCCACCATGACCATGATGAAATTTCTTATTGTTTCCCTTGTTTTGATTGCTTTCACCTCTCACCTATGTAAGAGCTTTGAGCTAGAAAGAAAGGATTTTGAATCTGAAAAAAGTTTGATGCAACTCTACAAGAGATGGAGTAGCCACCATAGAATCTCAAGGAATGCACATGAGACGGACAAACGTTTCAAGGTTTTCAAAGATAATGCAAAACATGTGTTCAAAGTGAACCACATGGGAAAATCATTAAAATTGAAGCTTAACCAATTTGCTGATATGTCTGATGATGAGTTTAGTAAGACATATGGTTCCAACATTACTTACTACAAAAACTTACATGCCAAGGCTGGTGGTCGTGTTGGTGGATTTATGTATGAACGAGCAACAAATATTCCATCTTCAATCGACTGGAGAAAAAAAGGAGCTGTGAATGCCATAAAAAATCAAGGCAGATGTGGTAATTAATTTAATTTAATTTATAATTCTTTCTAAACTCTAATGCCTTCTAACTAATAATGGTAATTAATTTAATTTAATTTATAATTCTTTCTAAACTCTAATGCCTTCTAACTAATAATTATGTGTTATGTGTTCCGCAGGAAGTTGTTGGGCGTTTGCAGCTGTGGCTGCCGTGGAATCTATTCACCAAATAAAAACAAATGAGTTAATATCTCTATCAGAGCAAGAAGTGGTGGATTGTGATTATAAAGTCGGTGGTTGTCGTGGAGGAGATTATAACTCTGCATTTGAGTTCATAATGGAAAATGGTGGAGTCACAATTGAGGATAACTATCCGTATTATGCCGGAGATGGATATTGTCGTAGACCAGGAGTGAGTTTAAATCAATATAATTCTAATTAAACTATCTAAATTTATTATATAAGTGAGTTATTGATCATAATATAATTATGTGTATTGTGTTTGAATTATACTTCACTAATAGCCTAATAATGAGAGAGTGACAATTGATGGATACGAGAACGTACCTCGAAACAATGAGTATGCTTTGATGAAAGCAGTTGCACACCAACCGGTAGCAGTGTCTATAGCGTCAAGTGGAAGTGACTTTAGATTTTATGGAGAGGCAAGTGTAGTTAATTTTGTTGTTATAATTTAATTATAATTTAATTATAATTTGGTGCAACATAATGAAAAGAAAAAATTGATAATTATTTGCAGGGAATGTTTACGGAAGAGAACTTTTGCGGAATTAGAATTGACCACACGGTAGTGGTAGTTGGGTACGGAAGTGATGAAGAGGGAGATTATTGGATAATAAGGAACCAATATGGAACTCAATGGGGAATGAATGGTTATATGAAGATGCAACGAGGAGCACAAAGCCCACAAGGTGTATGTGGAATGGCAATGCAACCTGCCTTTCCCGTCAAGTATTGATTTCCTATCTCTTTTTTATTAGAACTGGGTAGCTAGCT

mRNA sequence

AATTCATCCTATTATTCAAATCTTTCCACCATGACCATGATGAAATTTCTTATTGTTTCCCTTGTTTTGATTGCTTTCACCTCTCACCTATGTAAGAGCTTTGAGCTAGAAAGAAAGGATTTTGAATCTGAAAAAAGTTTGATGCAACTCTACAAGAGATGGAGTAGCCACCATAGAATCTCAAGGAATGCACATGAGACGGACAAACGTTTCAAGGTTTTCAAAGATAATGCAAAACATGTGTTCAAAGTGAACCACATGGGAAAATCATTAAAATTGAAGCTTAACCAATTTGCTGATATGTCTGATGATGAGTTTAGTAAGACATATGGTTCCAACATTACTTACTACAAAAACTTACATGCCAAGGCTGGTGGTCGTGTTGGTGGATTTATGTATGAACGAGCAACAAATATTCCATCTTCAATCGACTGGAGAAAAAAAGGAGCTGTGAATGCCATAAAAAATCAAGGCAGATGTGGAAGTTGTTGGGCGTTTGCAGCTGTGGCTGCCGTGGAATCTATTCACCAAATAAAAACAAATGAGTTAATATCTCTATCAGAGCAAGAAGTGGTGGATTGTGATTATAAAGTCGGTGGTTGTCGTGGAGGAGATTATAACTCTGCATTTGAGTTCATAATGGAAAATGGTGGAGTCACAATTGAGGATAACTATCCGTATTATGCCGGAGATGGATATTGTCGTAGACCAGGACCTAATAATGAGAGAGTGACAATTGATGGATACGAGAACGTACCTCGAAACAATGAGTATGCTTTGATGAAAGCAGTTGCACACCAACCGGTAGCAGTGTCTATAGCGTCAAGTGGAAGTGACTTTAGATTTTATGGAGAGGCAAGGAATGTTTACGGAAGAGAACTTTTGCGGAATTAGAATTGACCACACGGTAGTGGTAGTTGGGTACGGAAGTGATGAAGAGGGAGATTATTGGATAATAAGGAACCAATATGGAACTCAATGGGGAATGAATGGTTATATGAAGATGCAACGAGGAGCACAAAGCCCACAAGGTGTATGTGGAATGGCAATGCAACCTGCCTTTCCCGTCAAGTATTGATTTCCTATCTCTTTTTTATTAGAACTGGGTAGCTAGCT

Coding sequence (CDS)

AATTCATCCTATTATTCAAATCTTTCCACCATGACCATGATGAAATTTCTTATTGTTTCCCTTGTTTTGATTGCTTTCACCTCTCACCTATGTAAGAGCTTTGAGCTAGAAAGAAAGGATTTTGAATCTGAAAAAAGTTTGATGCAACTCTACAAGAGATGGAGTAGCCACCATAGAATCTCAAGGAATGCACATGAGACGGACAAACGTTTCAAGGTTTTCAAAGATAATGCAAAACATGTGTTCAAAGTGAACCACATGGGAAAATCATTAAAATTGAAGCTTAACCAATTTGCTGATATGTCTGATGATGAGTTTAGTAAGACATATGGTTCCAACATTACTTACTACAAAAACTTACATGCCAAGGCTGGTGGTCGTGTTGGTGGATTTATGTATGAACGAGCAACAAATATTCCATCTTCAATCGACTGGAGAAAAAAAGGAGCTGTGAATGCCATAAAAAATCAAGGCAGATGTGGAAGTTGTTGGGCGTTTGCAGCTGTGGCTGCCGTGGAATCTATTCACCAAATAAAAACAAATGAGTTAATATCTCTATCAGAGCAAGAAGTGGTGGATTGTGATTATAAAGTCGGTGGTTGTCGTGGAGGAGATTATAACTCTGCATTTGAGTTCATAATGGAAAATGGTGGAGTCACAATTGAGGATAACTATCCGTATTATGCCGGAGATGGATATTGTCGTAGACCAGGACCTAATAATGAGAGAGTGACAATTGATGGATACGAGAACGTACCTCGAAACAATGAGTATGCTTTGATGAAAGCAGTTGCACACCAACCGGTAGCAGTGTCTATAGCGTCAAGTGGAAGTGACTTTAGATTTTATGGAGAGGCAAGGAATGTTTACGGAAGAGAACTTTTGCGGAATTAGAATTGACCACACGGTAGTGGTAGTTGGGTACGGAAGTGATGAAGAGGGAGATTATTGGATAATAAGGAACCAATATGGAACTCAATGGGGAATGAATGGTTATATGAAGATGCAACGAGGAGCACAAAGCCCACAAGGTGTATGTGGAATGGCAATGCAACCTGCCTTTCCCGTCAAGTATTGATTTCCTATCTCTTTTTTATTAGAACTGGGTAGCTAGCT

Protein sequence

NSSYYSNLSTMTMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKRFKVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGGFMYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQEVVDCDYKVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYENVPRNNEYALMKAVAHQPVAVSIASSGSDFRFYGEARNVYGRELLRN*N*PHGSGSWVRK**RGRLLDNKEPIWNSMGNEWLYEDATRSTKPTRCMWNGNATCLSRQVLISYLFFIRTG*LA

Homology

[Close]

BLAST of Chy12G208620.1 vs. ExPASy Swiss-Prot
Match: O65039 (Vignain OS=Ricinus communis OX=3988 GN=CYSEP PE=1 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 7.5e-78
Identity = 149/275 (54.18%), Postives = 194/275 (70.55%), Query Frame = 0


Query: 13  MMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKRFK 72
           M KF++++L L A    + +SF+   K+ ESE+SL  LY+RW SHH +SR+ HE  KRF 
Sbjct: 1   MQKFILLALSL-ALVLAITESFDFHEKELESEESLWGLYERWRSHHTVSRSLHEKQKRFN 60

Query: 73  VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTY-GSNITYYKNLHAKAGGRVGGF 132
           VFK NA HV   N M K  KLKLN+FADM++ EF  TY GS + +++       G  G F
Sbjct: 61  VFKHNAMHVHNANKMDKPYKLKLNKFADMTNHEFRNTYSGSKVKHHRMFRGGPRGN-GTF 120

Query: 133 MYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQEV 192
           MYE+   +P+S+DWRKKGAV ++K+QG+CGSCWAF+ + AVE I+QIKTN+L+SLSEQE+
Sbjct: 121 MYEKVDTVPASVDWRKKGAVTSVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSEQEL 180

Query: 193 VDCDYKVG-GCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYE 252
           VDCD     GC GG  + AFEFI + GG+T E NYPY A DG C     N   V+IDG+E
Sbjct: 181 VDCDTDQNQGCNGGLMDYAFEFIKQRGGITTEANYPYEAYDGTCDVSKENAPAVSIDGHE 240

Query: 253 NVPRNNEYALMKAVAHQPVAVSIASSGSDFRFYGE 286
           NVP N+E AL+KAVA+QPV+V+I + GSDF+FY E
Sbjct: 241 NVPENDENALLKAVANQPVSVAIDAGGSDFQFYSE 273

[Close]

BLAST of Chy12G208620.1 vs. ExPASy Swiss-Prot
Match: P12412 (Vignain OS=Vigna mungo OX=3915 PE=1 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 1.8e-76
Identity = 150/277 (54.15%), Postives = 191/277 (68.95%), Query Frame = 0


Query: 11  MTMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKR 70
           M M K L V L L +    +  SF+   KD ESE+SL  LY+RW SHH +SR+  E  KR
Sbjct: 1   MAMKKLLWVVLSL-SLVLGVANSFDFHEKDLESEESLWDLYERWRSHHTVSRSLGEKHKR 60

Query: 71  FKVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTY-GSNITYYKNLHAKAGGRVG 130
           F VFK N  HV   N M K  KLKLN+FADM++ EF  TY GS + ++K       G  G
Sbjct: 61  FNVFKANVMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHHKMFRGSQHGS-G 120

Query: 131 GFMYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQ 190
            FMYE+  ++P+S+DWRKKGAV  +K+QG+CGSCWAF+ + AVE I+QIKTN+L+SLSEQ
Sbjct: 121 TFMYEKVGSVPASVDWRKKGAVTDVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSEQ 180

Query: 191 EVVDCDYKVG-GCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDG 250
           E+VDCD +   GC GG   SAFEFI + GG+T E NYPY A +G C     N+  V+IDG
Sbjct: 181 ELVDCDKEENQGCNGGLMESAFEFIKQKGGITTESNYPYTAQEGTCDESKVNDLAVSIDG 240

Query: 251 YENVPRNNEYALMKAVAHQPVAVSIASSGSDFRFYGE 286
           +ENVP N+E AL+KAVA+QPV+V+I + GSDF+FY E
Sbjct: 241 HENVPVNDENALLKAVANQPVSVAIDAGGSDFQFYSE 275

[Close]

BLAST of Chy12G208620.1 vs. ExPASy Swiss-Prot
Match: P25803 (Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2)

HSP 1 Score: 280.0 bits (715), Expect = 3.8e-74
Identity = 138/257 (53.70%), Postives = 177/257 (68.87%), Query Frame = 0


Query: 30  LCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKRFKVFKDNAKHVFKVNHMGK 89
           +  SF+   KD  SE+SL  LY+RW SHH +SR+  E  KRF VFK N  HV   N M K
Sbjct: 19  VANSFDFHDKDLASEESLWDLYERWRSHHTVSRSLGEKHKRFNVFKANLMHVHNTNKMDK 78

Query: 90  SLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGGFMYERATNIPSSIDWRKKG 149
             KLKLN+FADM++ EF  TY  +   +  +        G FMYE+  ++P S+DWRKKG
Sbjct: 79  PYKLKLNKFADMTNHEFRSTYAGSKVNHPRMFRGTPHENGAFMYEKVVSVPPSVDWRKKG 138

Query: 150 AVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQEVVDCDYKVG-GCRGGDYNS 209
           AV  +K+QG+CGSCWAF+ V AVE I+QIKTN+L++LSEQE+VDCD +   GC GG   S
Sbjct: 139 AVTDVKDQGQCGSCWAFSTVVAVEGINQIKTNKLVALSEQELVDCDKEENQGCNGGLMES 198

Query: 210 AFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYENVPRNNEYALMKAVAHQP 269
           AFEFI + GG+T E NYPY A +G C     N+  V+IDG+ENVP N+E AL+KAVA+QP
Sbjct: 199 AFEFIKQKGGITTESNYPYKAQEGTCDASKVNDLAVSIDGHENVPANDEDALLKAVANQP 258

Query: 270 VAVSIASSGSDFRFYGE 286
           V+V+I + GSDF+FY E
Sbjct: 259 VSVAIDAGGSDFQFYSE 275

[Close]

BLAST of Chy12G208620.1 vs. ExPASy Swiss-Prot
Match: Q9STL4 (KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana OX=3702 GN=CEP2 PE=1 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 3.2e-73
Identity = 143/276 (51.81%), Postives = 188/276 (68.12%), Query Frame = 0


Query: 13  MMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKRFK 72
           M K L++ L  +      C  F+ + K+ ESE+ L  LY RW SHH + R+ +E +KRF 
Sbjct: 1   MKKLLLIFLFSLVILQTAC-GFDYDDKEIESEEGLSTLYDRWRSHHSVPRSLNEREKRFN 60

Query: 73  VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTY-GSNITYYKNLHA-KAGGRVGG 132
           VF+ N  HV   N   +S KLKLN+FAD++ +EF   Y GSNI +++ L   K G +   
Sbjct: 61  VFRHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPKRGSKQFM 120

Query: 133 FMYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQE 192
           + +E  + +PSS+DWRKKGAV  IKNQG+CGSCWAF+ VAAVE I++IKTN+L+SLSEQE
Sbjct: 121 YDHENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLSEQE 180

Query: 193 VVDCDYKVG-GCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGY 252
           +VDCD K   GC GG    AFEFI +NGG+T ED+YPY   DG C     N   VTIDG+
Sbjct: 181 LVDCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTIDGH 240

Query: 253 ENVPRNNEYALMKAVAHQPVAVSIASSGSDFRFYGE 286
           E+VP N+E AL+KAVA+QPV+V+I +  SDF+FY E
Sbjct: 241 EDVPENDENALLKAVANQPVSVAIDAGSSDFQFYSE 275

[Close]

BLAST of Chy12G208620.1 vs. ExPASy Swiss-Prot
Match: Q9STL5 (KDEL-tailed cysteine endopeptidase CEP3 OS=Arabidopsis thaliana OX=3702 GN=CEP3 PE=2 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 1.2e-72
Identity = 139/270 (51.48%), Postives = 187/270 (69.26%), Query Frame = 0


Query: 21  LVLIAFTSHL--CKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKRFKVFKDNA 80
           +VLI+F S L   K F+ + K+ E+E+++ +LY+RW  HH +SR +HE  KRF VF+ N 
Sbjct: 6   IVLISFLSLLQASKGFDFDEKELETEENVWKLYERWRGHHSVSRASHEAIKRFNVFRHNV 65

Query: 81  KHVFKVNHMGKSLKLKLNQFADMSDDEFSKTY-GSNITYYKNLHAKAGGRVGGFMYERAT 140
            HV + N   K  KLK+N+FAD++  EF  +Y GSN+ +++ L     G  GGFMYE  T
Sbjct: 66  LHVHRTNKKNKPYKLKINRFADITHHEFRSSYAGSNVKHHRMLRGPKRGS-GGFMYENVT 125

Query: 141 NIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQEVVDCDYK 200
            +PSS+DWR+KGAV  +KNQ  CGSCWAF+ VAAVE I++I+TN+L+SLSEQE+VDCD +
Sbjct: 126 RVPSSVDWREKGAVTEVKNQQDCGSCWAFSTVAAVEGINKIRTNKLVSLSEQELVDCDTE 185

Query: 201 VG-GCRGGDYNSAFEFIMENGGVTIEDNYPYYAGD-GYCRRPGPNNERVTIDGYENVPRN 260
              GC GG    AFEFI  NGG+  E+ YPY + D  +CR      E VTIDG+E+VP N
Sbjct: 186 ENQGCAGGLMEPAFEFIKNNGGIKTEETYPYDSSDVQFCRANSIGGETVTIDGHEHVPEN 245

Query: 261 NEYALMKAVAHQPVAVSIASSGSDFRFYGE 286
           +E  L+KAVAHQPV+V+I +  SDF+ Y E
Sbjct: 246 DEEELLKAVAHQPVSVAIDAGSSDFQLYSE 274

[Close]

BLAST of Chy12G208620.1 vs. ExPASy TrEMBL
Match: A0A1S3BYQ3 (ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103494879 PE=3 SV=1)

HSP 1 Score: 487.6 bits (1254), Expect = 4.5e-134
Identity = 239/274 (87.23%), Postives = 253/274 (92.34%), Query Frame = 0


Query: 12  TMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKRF 71
           T+MKFLIV LVLIA TSHLC+SFELERKDFESEKSLMQLYKRWSSHHRISRNA+E  KRF
Sbjct: 3   TVMKFLIVPLVLIALTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKRF 62

Query: 72  KVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGGF 131
           KVFKDNAKHVFK NHMG+SLKL+LNQFADMSDDEFS  +GSNITYYKNLHAK  G VGGF
Sbjct: 63  KVFKDNAKHVFKKNHMGRSLKLQLNQFADMSDDEFSSIHGSNITYYKNLHAKT-GHVGGF 122

Query: 132 MYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQEV 191
           MYE A  IPSSIDWRKKGAVNAIKNQG CGSCWAFAAVAAVESIHQIKTNEL+SLSEQEV
Sbjct: 123 MYEHAKEIPSSIDWRKKGAVNAIKNQGGCGSCWAFAAVAAVESIHQIKTNELVSLSEQEV 182

Query: 192 VDCDYKVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYEN 251
           VDCDY+ GGCRGG YNSAFEF+MENGG+T+EDNYPYY GDGYCRR G  NERV IDGYEN
Sbjct: 183 VDCDYRDGGCRGGHYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVKIDGYEN 242

Query: 252 VPRNNEYALMKAVAHQPVAVSIASSGSDFRFYGE 286
           VPRNNE+ALMKAVAHQPVAV+IASSGSDFRFYG+
Sbjct: 243 VPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQ 275

[Close]

BLAST of Chy12G208620.1 vs. ExPASy TrEMBL
Match: A0A1S3BYU0 (ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103494878 PE=3 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 1.0e-133
Identity = 239/275 (86.91%), Postives = 255/275 (92.73%), Query Frame = 0


Query: 11  MTMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKR 70
           M +MKFLIV LVLIAFT HLC+SFELERKDFESEKSLMQLYKRWSSHHRISRNA+E  KR
Sbjct: 1   MAVMKFLIVPLVLIAFTFHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKR 60

Query: 71  FKVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGG 130
           FKVFKDNAK+VFK NHMG+SLKL+LNQFADMSDDEFS  +GSNITYYKNLHAK  GRVGG
Sbjct: 61  FKVFKDNAKYVFKKNHMGRSLKLQLNQFADMSDDEFSSIHGSNITYYKNLHAK-NGRVGG 120

Query: 131 FMYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQE 190
           FMYE A +IPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNEL+SLSEQE
Sbjct: 121 FMYEHANDIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVSLSEQE 180

Query: 191 VVDCDYKVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYE 250
           VVDCDY+  GC GG YNSAFEF+MENGG+T+EDNYPYY GDGYCRR G  NERVTIDGYE
Sbjct: 181 VVDCDYRDSGCLGGFYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVTIDGYE 240

Query: 251 NVPRNNEYALMKAVAHQPVAVSIASSGSDFRFYGE 286
           NVPRNNE+ALMKAVAHQPVAV+IASSGSDFRFYG+
Sbjct: 241 NVPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQ 274

[Close]

BLAST of Chy12G208620.1 vs. ExPASy TrEMBL
Match: A0A5A7TM64 (Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003430 PE=3 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 1.3e-133
Identity = 238/274 (86.86%), Postives = 252/274 (91.97%), Query Frame = 0


Query: 12  TMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKRF 71
           T+MKFLIV  VLIA TSHLC+SFELERKDFESEKSLMQLYKRWSSHHRISRNA+E  KRF
Sbjct: 3   TVMKFLIVPFVLIALTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKRF 62

Query: 72  KVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGGF 131
           KVFKDNAKHVFK NHMG+SLKL+LNQFADMSDDEFS  +GSNITYYKNLHAK  G VGGF
Sbjct: 63  KVFKDNAKHVFKKNHMGRSLKLQLNQFADMSDDEFSSIHGSNITYYKNLHAKT-GHVGGF 122

Query: 132 MYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQEV 191
           MYE A  IPSSIDWRKKGAVNAIKNQG CGSCWAFAAVAAVESIHQIKTNEL+SLSEQEV
Sbjct: 123 MYEHAKEIPSSIDWRKKGAVNAIKNQGGCGSCWAFAAVAAVESIHQIKTNELVSLSEQEV 182

Query: 192 VDCDYKVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYEN 251
           VDCDY+ GGCRGG YNSAFEF+MENGG+T+EDNYPYY GDGYCRR G  NERV IDGYEN
Sbjct: 183 VDCDYRDGGCRGGHYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVKIDGYEN 242

Query: 252 VPRNNEYALMKAVAHQPVAVSIASSGSDFRFYGE 286
           VPRNNE+ALMKAVAHQPVAV+IASSGSDFRFYG+
Sbjct: 243 VPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQ 275

[Close]

BLAST of Chy12G208620.1 vs. ExPASy TrEMBL
Match: A0A5A7U0D8 (Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00020 PE=3 SV=1)

HSP 1 Score: 478.4 bits (1230), Expect = 2.7e-131
Identity = 237/284 (83.45%), Postives = 256/284 (90.14%), Query Frame = 0


Query: 11  MTMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKR 70
           MT+MKF IV+LVLIAF SHLC+SF+LERKDF SEKSLMQLYKRWSSHHRISRNA+E  KR
Sbjct: 1   MTVMKFFIVALVLIAFMSHLCESFKLERKDFASEKSLMQLYKRWSSHHRISRNANEMHKR 60

Query: 71  FKVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGG 130
           FKVFKDNAKHVFKVN MG+SLKL+LNQFADMSDDEFS  + SNITYYK+LHAK  G  G 
Sbjct: 61  FKVFKDNAKHVFKVNQMGRSLKLRLNQFADMSDDEFSSIHSSNITYYKHLHAKTVG--GS 120

Query: 131 FMYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQE 190
           FMYE A  IPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNEL+SLSEQE
Sbjct: 121 FMYEHAKEIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVSLSEQE 180

Query: 191 VVDCDYKVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYE 250
           VVDCDY+ GGCRGG YNSAFEF+MENGG+T+EDNYPYY GDGYCRR G  NERVTIDGYE
Sbjct: 181 VVDCDYRDGGCRGGFYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVTIDGYE 240

Query: 251 NVPRNNEYALMKAVAHQPVAVSIASSGSDFRFYGEARNV-YGRE 294
           NVPRNNE+ALMKAVAHQPVAV+IAS GSDF+FYG+A  V YG +
Sbjct: 241 NVPRNNEHALMKAVAHQPVAVAIASGGSDFKFYGQAMVVGYGTD 282

[Close]

BLAST of Chy12G208620.1 vs. ExPASy TrEMBL
Match: A0A0A0KGB1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G490180 PE=3 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 5.1e-130
Identity = 232/278 (83.45%), Postives = 252/278 (90.65%), Query Frame = 0


Query: 11  MTMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKR 70
           MT+MKFLIV LVL+AF+ ++C+SFELERKDFESEKSLMQLYKRWSSHHRISRNA+E   R
Sbjct: 1   MTVMKFLIVPLVLVAFSCNICESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHNR 60

Query: 71  FKVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAK----AGG 130
           FKVFK+NAKHVFKVN MGKSLKLKLNQFADMSDDEF   Y SNITYYK+LHAK     GG
Sbjct: 61  FKVFKNNAKHVFKVNLMGKSLKLKLNQFADMSDDEFRNMYSSNITYYKDLHAKKIEATGG 120

Query: 131 RVGGFMYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISL 190
           R+GGFMYE A NIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNEL+SL
Sbjct: 121 RIGGFMYEHANNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVSL 180

Query: 191 SEQEVVDCDYKVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTI 250
           SE+EV+DCDY+ GGCRGG YNSAFEF+M+N GVTIEDNYPYY G+GYCRR G  N+RV I
Sbjct: 181 SEEEVLDCDYRDGGCRGGFYNSAFEFMMDNDGVTIEDNYPYYEGNGYCRRRGGRNKRVRI 240

Query: 251 DGYENVPRNNEYALMKAVAHQPVAVSIASSGSDFRFYG 285
           DGYENVPRNNEYALMKAVAHQPVAV+IAS GSDF+FYG
Sbjct: 241 DGYENVPRNNEYALMKAVAHQPVAVAIASGGSDFKFYG 278

[Close]

BLAST of Chy12G208620.1 vs. NCBI nr
Match: XP_031739597.1 (ervatamin-B-like [Cucumis sativus] >KAE8649107.1 hypothetical protein Csa_014529 [Cucumis sativus])

HSP 1 Score: 508 bits (1308), Expect = 5.72e-178
Identity = 247/277 (89.17%), Postives = 264/277 (95.31%), Query Frame = 0


Query: 11  MTMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKR 70
           MTMMKFLIV +VLIAFTSHLC+SFELERKDFESEKSLMQLYKRWSSHHRISRNAHE  KR
Sbjct: 1   MTMMKFLIVFVVLIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKR 60

Query: 71  FKVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGG--RV 130
           FK+F+DNAKHVF+VNHMGKSLKL+LNQFAD+SDDEFS  YGSNIT+Y NLHAKAGG  RV
Sbjct: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYDNLHAKAGGGGRV 120

Query: 131 GGFMYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSE 190
           GGFMYERA  IPSSIDWR+KGAVNAIKNQG CGSCWAFAAVAAVESIHQIKTNEL+SLSE
Sbjct: 121 GGFMYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSE 180

Query: 191 QEVVDCDYKVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDG 250
           QEVVDCDYKVGGCRGGDYNSAFEFIM+NGG+TIE+NYPY+AG+GYCRR GPNNERVTIDG
Sbjct: 181 QEVVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDG 240

Query: 251 YENVPRNNEYALMKAVAHQPVAVSIASSGSDFRFYGE 285
           YE VPRNNEYALMKAVAHQPVAV++ASSGSDFRFYGE
Sbjct: 241 YERVPRNNEYALMKAVAHQPVAVAVASSGSDFRFYGE 277

[Close]

BLAST of Chy12G208620.1 vs. NCBI nr
Match: XP_031740503.1 (ervatamin-B [Cucumis sativus])

HSP 1 Score: 505 bits (1301), Expect = 6.18e-177
Identity = 243/275 (88.36%), Postives = 261/275 (94.91%), Query Frame = 0


Query: 11  MTMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKR 70
           MTMMKFLIV +VLIAF SHLC+ F+LERKDFESEKSLMQLYKRWSSHHRISRNAHE  KR
Sbjct: 1   MTMMKFLIVFVVLIAFASHLCEGFDLERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKR 60

Query: 71  FKVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGG 130
           FK+F+DNAK VFKVNHMGKSLKL+LNQFAD+SDDEFS  YGSNIT+Y NLHAKAGGRVGG
Sbjct: 61  FKIFQDNAKRVFKVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNNLHAKAGGRVGG 120

Query: 131 FMYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQE 190
           FMYERA NIP SIDWR+KGAVNAIKNQG CGSCWAFAAVAAVESIHQIKTNEL+SLSEQE
Sbjct: 121 FMYERAMNIPFSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSEQE 180

Query: 191 VVDCDYKVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYE 250
           VVDCDYKVGGCRGGDYNSAFEFIM+NGG+TIE+NYPY+AG+GYCRR GPN+ERVTIDGYE
Sbjct: 181 VVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYE 240

Query: 251 NVPRNNEYALMKAVAHQPVAVSIASSGSDFRFYGE 285
            VP+NNEYALMKAVAHQPVAVS+ASSGSDFRFYGE
Sbjct: 241 RVPQNNEYALMKAVAHQPVAVSVASSGSDFRFYGE 275

[Close]

BLAST of Chy12G208620.1 vs. NCBI nr
Match: XP_031740474.1 (ervatamin-B [Cucumis sativus] >KAE8649110.1 hypothetical protein Csa_014565 [Cucumis sativus])

HSP 1 Score: 490 bits (1262), Expect = 4.74e-171
Identity = 237/275 (86.18%), Postives = 261/275 (94.91%), Query Frame = 0


Query: 11  MTMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKR 70
           MTMMKFLIV +VLIAFTSHLC+SFELE KDFESE+SLMQLYKRWSSHHRISRNAHE  KR
Sbjct: 1   MTMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKR 60

Query: 71  FKVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGG 130
           FK+F+DNAKHVF+VNHMGKSLKL+LNQFAD+SDDEFS  YGSNIT+Y +LHA    RVGG
Sbjct: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNSLHAN---RVGG 120

Query: 131 FMYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQE 190
           FMYERA NIPSSIDWR++GAVNAIKNQGRCGSCWAFAAVAAVESIHQI+TNEL+SLSEQE
Sbjct: 121 FMYERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQE 180

Query: 191 VVDCDYKVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYE 250
           VVDCDYKVGGCRGG+Y+SAFEFIM+NGG+TIE+NYPY+AG+GYCRR GPN+ERVTIDGYE
Sbjct: 181 VVDCDYKVGGCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYE 240

Query: 251 NVPRNNEYALMKAVAHQPVAVSIASSGSDFRFYGE 285
            VP+NNEYALMKAVAHQPVAVS+ASSGSDFRFYGE
Sbjct: 241 CVPQNNEYALMKAVAHQPVAVSVASSGSDFRFYGE 272

[Close]

BLAST of Chy12G208620.1 vs. NCBI nr
Match: XP_008454483.1 (PREDICTED: ervatamin-B-like [Cucumis melo])

HSP 1 Score: 486 bits (1252), Expect = 1.82e-169
Identity = 239/274 (87.23%), Postives = 253/274 (92.34%), Query Frame = 0


Query: 12  TMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKRF 71
           T+MKFLIV LVLIA TSHLC+SFELERKDFESEKSLMQLYKRWSSHHRISRNA+E  KRF
Sbjct: 3   TVMKFLIVPLVLIALTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKRF 62

Query: 72  KVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGGF 131
           KVFKDNAKHVFK NHMG+SLKL+LNQFADMSDDEFS  +GSNITYYKNLHAK G  VGGF
Sbjct: 63  KVFKDNAKHVFKKNHMGRSLKLQLNQFADMSDDEFSSIHGSNITYYKNLHAKTG-HVGGF 122

Query: 132 MYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQEV 191
           MYE A  IPSSIDWRKKGAVNAIKNQG CGSCWAFAAVAAVESIHQIKTNEL+SLSEQEV
Sbjct: 123 MYEHAKEIPSSIDWRKKGAVNAIKNQGGCGSCWAFAAVAAVESIHQIKTNELVSLSEQEV 182

Query: 192 VDCDYKVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYEN 251
           VDCDY+ GGCRGG YNSAFEF+MENGG+T+EDNYPYY GDGYCRR G  NERV IDGYEN
Sbjct: 183 VDCDYRDGGCRGGHYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVKIDGYEN 242

Query: 252 VPRNNEYALMKAVAHQPVAVSIASSGSDFRFYGE 285
           VPRNNE+ALMKAVAHQPVAV+IASSGSDFRFYG+
Sbjct: 243 VPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQ 275

[Close]

BLAST of Chy12G208620.1 vs. NCBI nr
Match: XP_008454482.1 (PREDICTED: ervatamin-B-like [Cucumis melo])

HSP 1 Score: 486 bits (1250), Expect = 3.53e-169
Identity = 239/275 (86.91%), Postives = 255/275 (92.73%), Query Frame = 0


Query: 11  MTMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKR 70
           M +MKFLIV LVLIAFT HLC+SFELERKDFESEKSLMQLYKRWSSHHRISRNA+E  KR
Sbjct: 1   MAVMKFLIVPLVLIAFTFHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKR 60

Query: 71  FKVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGG 130
           FKVFKDNAK+VFK NHMG+SLKL+LNQFADMSDDEFS  +GSNITYYKNLHAK  GRVGG
Sbjct: 61  FKVFKDNAKYVFKKNHMGRSLKLQLNQFADMSDDEFSSIHGSNITYYKNLHAK-NGRVGG 120

Query: 131 FMYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQE 190
           FMYE A +IPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNEL+SLSEQE
Sbjct: 121 FMYEHANDIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVSLSEQE 180

Query: 191 VVDCDYKVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYE 250
           VVDCDY+  GC GG YNSAFEF+MENGG+T+EDNYPYY GDGYCRR G  NERVTIDGYE
Sbjct: 181 VVDCDYRDSGCLGGFYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVTIDGYE 240

Query: 251 NVPRNNEYALMKAVAHQPVAVSIASSGSDFRFYGE 285
           NVPRNNE+ALMKAVAHQPVAV+IASSGSDFRFYG+
Sbjct: 241 NVPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQ 274

[Close]

BLAST of Chy12G208620.1 vs. TAIR 10
Match: AT3G48340.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 276.9 bits (707), Expect = 2.3e-74
Identity = 143/276 (51.81%), Postives = 188/276 (68.12%), Query Frame = 0


Query: 13  MMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKRFK 72
           M K L++ L  +      C  F+ + K+ ESE+ L  LY RW SHH + R+ +E +KRF 
Sbjct: 1   MKKLLLIFLFSLVILQTAC-GFDYDDKEIESEEGLSTLYDRWRSHHSVPRSLNEREKRFN 60

Query: 73  VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTY-GSNITYYKNLHA-KAGGRVGG 132
           VF+ N  HV   N   +S KLKLN+FAD++ +EF   Y GSNI +++ L   K G +   
Sbjct: 61  VFRHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPKRGSKQFM 120

Query: 133 FMYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQE 192
           + +E  + +PSS+DWRKKGAV  IKNQG+CGSCWAF+ VAAVE I++IKTN+L+SLSEQE
Sbjct: 121 YDHENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLSEQE 180

Query: 193 VVDCDYKVG-GCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGY 252
           +VDCD K   GC GG    AFEFI +NGG+T ED+YPY   DG C     N   VTIDG+
Sbjct: 181 LVDCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTIDGH 240

Query: 253 ENVPRNNEYALMKAVAHQPVAVSIASSGSDFRFYGE 286
           E+VP N+E AL+KAVA+QPV+V+I +  SDF+FY E
Sbjct: 241 EDVPENDENALLKAVANQPVSVAIDAGSSDFQFYSE 275

[Close]

BLAST of Chy12G208620.1 vs. TAIR 10
Match: AT3G48350.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 275.0 bits (702), Expect = 8.8e-74
Identity = 139/270 (51.48%), Postives = 187/270 (69.26%), Query Frame = 0


Query: 21  LVLIAFTSHL--CKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKRFKVFKDNA 80
           +VLI+F S L   K F+ + K+ E+E+++ +LY+RW  HH +SR +HE  KRF VF+ N 
Sbjct: 6   IVLISFLSLLQASKGFDFDEKELETEENVWKLYERWRGHHSVSRASHEAIKRFNVFRHNV 65

Query: 81  KHVFKVNHMGKSLKLKLNQFADMSDDEFSKTY-GSNITYYKNLHAKAGGRVGGFMYERAT 140
            HV + N   K  KLK+N+FAD++  EF  +Y GSN+ +++ L     G  GGFMYE  T
Sbjct: 66  LHVHRTNKKNKPYKLKINRFADITHHEFRSSYAGSNVKHHRMLRGPKRGS-GGFMYENVT 125

Query: 141 NIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQEVVDCDYK 200
            +PSS+DWR+KGAV  +KNQ  CGSCWAF+ VAAVE I++I+TN+L+SLSEQE+VDCD +
Sbjct: 126 RVPSSVDWREKGAVTEVKNQQDCGSCWAFSTVAAVEGINKIRTNKLVSLSEQELVDCDTE 185

Query: 201 VG-GCRGGDYNSAFEFIMENGGVTIEDNYPYYAGD-GYCRRPGPNNERVTIDGYENVPRN 260
              GC GG    AFEFI  NGG+  E+ YPY + D  +CR      E VTIDG+E+VP N
Sbjct: 186 ENQGCAGGLMEPAFEFIKNNGGIKTEETYPYDSSDVQFCRANSIGGETVTIDGHEHVPEN 245

Query: 261 NEYALMKAVAHQPVAVSIASSGSDFRFYGE 286
           +E  L+KAVAHQPV+V+I +  SDF+ Y E
Sbjct: 246 DEEELLKAVAHQPVSVAIDAGSSDFQLYSE 274

[Close]

BLAST of Chy12G208620.1 vs. TAIR 10
Match: AT5G50260.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 266.9 bits (681), Expect = 2.4e-71
Identity = 138/275 (50.18%), Postives = 183/275 (66.55%), Query Frame = 0


Query: 13  MMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKRFK 72
           M +F++++L ++       K  +   KD ESE SL +LY+RW SHH ++R+  E  KRF 
Sbjct: 1   MKRFIVLALCML-MVLETTKGLDFHNKDVESENSLWELYERWRSHHTVARSLEEKAKRFN 60

Query: 73  VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTY-GSNITYYKNLHAKAGGRVGGF 132
           VFK N KH+ + N   KS KLKLN+F DM+ +EF +TY GSNI +++    +       F
Sbjct: 61  VFKHNVKHIHETNKKDKSYKLKLNKFGDMTSEEFRRTYAGSNIKHHRMFQGEKKA-TKSF 120

Query: 133 MYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQEV 192
           MY     +P+S+DWRK GAV  +KNQG+CGSCWAF+ V AVE I+QI+T +L SLSEQE+
Sbjct: 121 MYANVNTLPTSVDWRKNGAVTPVKNQGQCGSCWAFSTVVAVEGINQIRTKKLTSLSEQEL 180

Query: 193 VDCDYKVG-GCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYE 252
           VDCD     GC GG  + AFEFI E GG+T E  YPY A D  C     N   V+IDG+E
Sbjct: 181 VDCDTNQNQGCNGGLMDLAFEFIKEKGGLTSELVYPYKASDETCDTNKENAPVVSIDGHE 240

Query: 253 NVPRNNEYALMKAVAHQPVAVSIASSGSDFRFYGE 286
           +VP+N+E  LMKAVA+QPV+V+I + GSDF+FY E
Sbjct: 241 DVPKNSEDDLMKAVANQPVSVAIDAGGSDFQFYSE 273

[Close]

BLAST of Chy12G208620.1 vs. TAIR 10
Match: AT1G20850.1 (xylem cysteine peptidase 2 )

HSP 1 Score: 219.9 bits (559), Expect = 3.3e-57
Identity = 118/267 (44.19%), Postives = 167/267 (62.55%), Query Frame = 0


Query: 20  SLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRW-SSHHRISRNAHETDKRFKVFKDNA 79
           SL L   +SH         +D ES   L++L++ W S+  +      E   RF+VFKDN 
Sbjct: 20  SLSLSFASSHDYSIVGYSPEDLESHDKLIELFENWISNFEKAYETVEEKFLRFEVFKDNL 79

Query: 80  KHVFKVNHMGKSLKLKLNQFADMSDDEFSKTY-GSNITYYKNLHAKAGGRVGGFMYERAT 139
           KH+ + N  GKS  L LN+FAD+S +EF K Y G      +    ++      F Y    
Sbjct: 80  KHIDETNKKGKSYWLGLNEFADLSHEEFKKMYLGLKTDIVRRDEERS---YAEFAYRDVE 139

Query: 140 NIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQEVVDCDYK 199
            +P S+DWRKKGAV  +KNQG CGSCWAF+ VAAVE I++I T  L +LSEQE++DCD  
Sbjct: 140 AVPKSVDWRKKGAVAEVKNQGSCGSCWAFSTVAAVEGINKIVTGNLTTLSEQELIDCDTT 199

Query: 200 V-GGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYENVPRNN 259
              GC GG  + AFE+I++NGG+  E++YPY   +G C      +E VTI+G+++VP N+
Sbjct: 200 YNNGCNGGLMDYAFEYIVKNGGLRKEEDYPYSMEEGTCEMQKDESETVTINGHQDVPTND 259

Query: 260 EYALMKAVAHQPVAVSIASSGSDFRFY 284
           E +L+KA+AHQP++V+I +SG +F+FY
Sbjct: 260 EKSLLKALAHQPLSVAIDASGREFQFY 283

[Close]

BLAST of Chy12G208620.1 vs. TAIR 10
Match: AT5G45890.1 (senescence-associated gene 12 )

HSP 1 Score: 216.9 bits (551), Expect = 2.8e-56
Identity = 117/267 (43.82%), Postives = 165/267 (61.80%), Query Frame = 0


Query: 22  VLIAFTSHLCKSFELERKDFESEKSLMQLYKRW-SSHHRISRNAHETDKRFKVFKDNAKH 81
           + +A  S  C S  L R   ++E  + + +  W + H R+  +  E + R+ VFK+N + 
Sbjct: 10  LFVAIFSSFCFSITLSR-PLDNELIMQKRHIEWMTKHGRVYADVKEENNRYVVFKNNVER 69

Query: 82  VFKVNHM--GKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGGFMYERATN 141
           +  +N +  G++ KL +NQFAD+++DEF   Y +       L +++  ++  F Y+  ++
Sbjct: 70  IEHLNSIPAGRTFKLAVNQFADLTNDEFRSMY-TGFKGVSALSSQSQTKMSPFRYQNVSS 129

Query: 142 --IPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQEVVDCDY 201
             +P S+DWRKKGAV  IKNQG CG CWAF+AVAA+E   QIK  +LISLSEQ++VDCD 
Sbjct: 130 GALPVSVDWRKKGAVTPIKNQGSCGCCWAFSAVAAIEGATQIKKGKLISLSEQQLVDCDT 189

Query: 202 KVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYENVPRNN 261
              GC GG  ++AFE I   GG+T E NYPY   D  C     N +  +I GYE+VP N+
Sbjct: 190 NDFGCEGGLMDTAFEHIKATGGLTTESNYPYKGEDATCNSKKTNPKATSITGYEDVPVND 249

Query: 262 EYALMKAVAHQPVAVSIASSGSDFRFY 284
           E ALMKAVAHQPV+V I   G DF+FY
Sbjct: 250 EQALMKAVAHQPVSVGIEGGGFDFQFY 274

The following BLAST results are available for this feature:

BLAST of Chy12G208620.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O65039	7.5e-78	54.18	Vignain OS=Ricinus communis OX=3988 GN=CYSEP PE=1 SV=1	[more]
P12412	1.8e-76	54.15	Vignain OS=Vigna mungo OX=3915 PE=1 SV=1	[more]
P25803	3.8e-74	53.70	Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2	[more]
Q9STL4	3.2e-73	51.81	KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana OX=3702 GN=CEP2 ...	[more]
Q9STL5	1.2e-72	51.48	KDEL-tailed cysteine endopeptidase CEP3 OS=Arabidopsis thaliana OX=3702 GN=CEP3 ...	[more]

BLAST of Chy12G208620.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BYQ3	4.5e-134	87.23	ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103494879 PE=3 SV=1	[more]
A0A1S3BYU0	1.0e-133	86.91	ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103494878 PE=3 SV=1	[more]
A0A5A7TM64	1.3e-133	86.86	Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G0034...	[more]
A0A5A7U0D8	2.7e-131	83.45	Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00...	[more]
A0A0A0KGB1	5.1e-130	83.45	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G490180 PE=3 SV=1	[more]

BLAST of Chy12G208620.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031739597.1	5.72e-178	89.17	ervatamin-B-like [Cucumis sativus] >KAE8649107.1 hypothetical protein Csa_014529...	[more]
XP_031740503.1	6.18e-177	88.36	ervatamin-B [Cucumis sativus]	[more]
XP_031740474.1	4.74e-171	86.18	ervatamin-B [Cucumis sativus] >KAE8649110.1 hypothetical protein Csa_014565 [Cuc...	[more]
XP_008454483.1	1.82e-169	87.23	PREDICTED: ervatamin-B-like [Cucumis melo]	[more]
XP_008454482.1	3.53e-169	86.91	PREDICTED: ervatamin-B-like [Cucumis melo]	[more]

BLAST of Chy12G208620.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G48340.1	2.3e-74	51.81	Cysteine proteinases superfamily protein	[more]
AT3G48350.1	8.8e-74	51.48	Cysteine proteinases superfamily protein	[more]
AT5G50260.1	2.4e-71	50.18	Cysteine proteinases superfamily protein	[more]
AT1G20850.1	3.3e-57	44.19	xylem cysteine peptidase 2	[more]
AT5G45890.1	2.8e-56	43.82	senescence-associated gene 12	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000668	Peptidase C1A, papain C-terminal	SMART	SM00645	pept_c1	coord: 139..339 e-value: 4.3E-48 score: 175.8
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 139..286 e-value: 1.3E-46 score: 159.4
IPR013201	Cathepsin propeptide inhibitor domain (I29)	SMART	SM00848	Inhibitor_I29_2	coord: 51..106 e-value: 1.0E-8 score: 45.0
IPR013201	Cathepsin propeptide inhibitor domain (I29)	PFAM	PF08246	Inhibitor_I29	coord: 51..106 e-value: 1.4E-9 score: 38.3
None	No IPR available	GENE3D	3.90.70.10	Cysteine proteinases	coord: 24..327 e-value: 1.1E-77 score: 263.9
None	No IPR available	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 31..302
None	No IPR available	PANTHER	PTHR12411:SF710	THIOL PROTEASE SEN102-LIKE	coord: 31..302
IPR000169	Cysteine peptidase, cysteine active site	PROSITE	PS00139	THIOL_PROTEASE_CYS	coord: 157..168
IPR039417	Papain-like cysteine endopeptidase	CDD	cd02248	Peptidase_C1A	coord: 140..283 e-value: 2.06263E-63 score: 199.387
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 44..285

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Chy12G208620	Chy12G208620	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Chy12G208620.1.exon1	Chy12G208620.1.exon1	exon
Chy12G208620.1.exon2	Chy12G208620.1.exon2	exon
Chy12G208620.1.exon3	Chy12G208620.1.exon3	exon
Chy12G208620.1.exon4	Chy12G208620.1.exon4	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Chy12G208620.1.cds1	Chy12G208620.1.cds1	CDS
Chy12G208620.1.cds2	Chy12G208620.1.cds2	CDS
Chy12G208620.1.cds3	Chy12G208620.1.cds3	CDS
Chy12G208620.1.cds4	Chy12G208620.1.cds4	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Chy12G208620.1	Chy12G208620.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
biological_process	GO:0018108	peptidyl-tyrosine phosphorylation
biological_process	GO:0051603	proteolysis involved in cellular protein catabolic process
cellular_component	GO:0005615	extracellular space
cellular_component	GO:0005764	lysosome
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0008234	cysteine-type peptidase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004197	cysteine-type endopeptidase activity
molecular_function	GO:0004713	protein tyrosine kinase activity