Chy12G207220.1 (mRNA) Cucumber (hystrix) v1

Overview


Name	Chy12G207220.1
Type	mRNA
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Mitogen-activated protein kinase
Location	chrH12: 1329932 .. 1332968 (+)
Sequence length	924
RNA-Seq Expression	Chy12G207220.1
Synteny	Chy12G207220.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTGCGGTTAAGAAGATTGCTAACGCGTTTGATAACCATATGGATGCGAAGAGGACGCTCCGTGAGATTAAGCTTCTACGCCATTTGGATCATGAAAATGTAAGGGACTTTATCCTTGTAGATTTGCTAAAACTGTTGTTTCTGTTAAATCATTGCGAATTTTGATTTCCTCTGTTTGTGTTTGTGATTTCTATTTTGATGAGTAAATTGGTGGCTGGGGGTAATGATGATAGATTTTTATGAAATTGACAAATAAAGATAGCAAGAAAACGTAAATGATCGGCACGGATTTACTTAGTTCACTGACATCGTTTTAGCTACGTTTACCAGTAGAGGGAACGATCGGTTTGGTTTATTTTTTGAGAGAACTATTAGATAATACCCATTGTAAATGTGCCAGATTTAAAGAGTTCATATATAGTGCATCCCTCTAGCCAAAACGTAAATAACATAAATCTAGATACAACTTAGGTTTGGTCATTGCGTTAGATGATTGATTCAAGGCATTCCAACATCCTAGAAGGTTCATTTTCATTGAATACAATTTTGATGTTGGAATTCCAACAACATTACTGATGAACCAACCCACTTATTGATGAACCAACCCAATATCATATGGAATACATTACTTTTGGTGTCTTGGTTCATCAATAAGTGGGTTGGACTTTGTCAGTACTTAGCCGCAGCAATGGTTGGGTGATTTGGCCATATACTAGTTGAATAACAGCTGTAGTAGCATGTGGTCTTAATTATTTTGTTGCTGATGGAAAGTTTATGGGATTTTGACTTTGTTACCTTATAATAATTGGACGATGAATTGGAATTGCTTTGTACAATGGTTATCTTGCTTTCTCTGTACTTTCTGATTGTATATTTAAAATCTTGGAGAAAGATTCTTAATTTTGTCCTTTAGGGTGAGAAGAACAATCTTATATAGATTGTGATTCATTGAATGTAGGTAATAGGCATAAGAGATGTGATTCCTCCACCTTTACGGAGAGAATTCAATGATGTCTACATTTCGACTGAACTAATGGATACTGATCTTCACCAAATAATCCGCTCCAACCAAAGTTTATCAGAAGAGCATTGTCAGGTACGATGGGGTCTTGACTTTTCATCATTCATTATGTTCCATTGCACCTTTGGTTTTTACTTTCAATTGTTCCACTTGTAATAATATAAGCTTTGGCATGCTTTGCGCAGTATTTCCTTTATCAGATTCTCAGAGGACTGAAATACATACATTCGGCAAATGTCATTCATCGAGACTTGAAACCGAGCAATCTATTGCTTAATGCAAACTGTGATCTTAAAATATGTGACTTTGGTCTTGCTCGACCAACTTCTGAAAATGAATGTATGACGGAATATGTTGTGACAAGATGGTACAGAGCACCTGAGCTTCTTTTGAACTCTGATTATACAGCTGCAATCGATATATGGTCTGTTGGTTGCATCTTTCTGGAGCTTATGAATAGAAGGCCTTTATTTCCAGGCAGGGATCATGTGCATCAGATGAGATTATTGACTGAGGTAAAACAATCTACAGGGAGCTCATGCAGTTTATCAAACTTAGAGTATCTAATTTTGACCATTAAAGATCTAGCTGATGAGAAAATAAACCCGAGAATTATTGATGTTCTAGATAATTTCTACTATCAGTTCTGTTGAGGATGCATCAGTTCATTACATGTGATATCTTTTGAAAAACTATACGATATATAATCCTTAATTTTGATAAGTATGGCACTCATTCAAAAGCAAGTCCTTGGTTCTGTTTTGATAGTTCTTATCAGTTTCCATTTGAAATATTGGTTTTGGCCAATGATTTTCAAGTTCTATTTTTATACAGACAAATGGCTCATTCTCTTTCCTTTTTCCTTCTATTGATTAGCATTGACTGTAAATTATACGCCGTCTCATGTTTTTTCATTTGAATTCTCAGCTTCTTGGCACACCAAGCGAGTCAGATCTTGGTTTTATCCGAAATGAAGACTCGAAAAGATATCTTCGACAGCTACCTCCACATCCTCGTCAGCCATTAGCAACGGTTTTTCCACATGTTCATCCGTTAGCAATTGATCTTGTGGATAAAATGTTGACATTTGACCCAACAAAGAGAATTACCGGTAAACATTTATTGAATGTCCATTACTTTGTCTTTTGATGGTTTGGATGAAATAATTGCTTGTTAGCTGCAATGTTAATGGTAGTTTAGTGATTGGATTGTTTAGAGTTGAATACTTTTAGTTATGACCTATTCATTTTCTTACAATATAATTAGGTTCAAGGTGCAAACTATCATAAATTTGCACGTGAAATAGCTTTATGTGCCTGCCGTGAGTTCAAACCGTAGTATTGGGTTATAAAGATTCAACAAATAAAGAAAAGGGAGAAATTGTAAACAGAAAAGATTGAGATTGAAATTTACGTGATTCACTGACAATGTGTTAGAAAATGTAAATATAGTAAAGATCAAACTCGAAATTTACGTGATTCACTAACAGTGTGCTAGTTATTTTCACAGATTGTGGAATAGATCAACTTATTATTAGAGAGTAATATTAGATGATATACAGATTGATGAGGTACCTCTAAGGTTAGAGAGTTTATATATAATGCACATCTTTAAACCTTTGGGCCAAACTTGTAATAAATGCATATATTGACAATAGATGCAACGCATTCCAACACATAGGGTGTCCATTCTCATGACATTTCAGCATCGTAGCAAGTTATATGATTATCCTATTGTTGGTCATAATCTTCATGTGTGCATTTGTCTTTTGAACACGGTTGAAACAATTTGTTATCTTCATTTTATTCCTTGATTTAACTTTATGAATTTGTCTTTTGAACAGTTGAAGAAGCGTTAGCACATCCATACCTAGAAAGATTGCATGACATAGCCGATGAGCCGGTTTGCTCAGAGCCATTCTCGTTTGAGTTTGAGCAACAATACTTAGACGAAGAACAGATGAAGGAGATGATTTACAGAGAATCATTGGCACTCAATCCAGAATTTGCATGA

mRNA sequence

ATGGTTGCGGTTAAGAAGATTGCTAACGCGTTTGATAACCATATGGATGCGAAGAGGACGCTCCGTGAGATTAAGCTTCTACGCCATTTGGATCATGAAAATGTAATAGGCATAAGAGATGTGATTCCTCCACCTTTACGGAGAGAATTCAATGATGTCTACATTTCGACTGAACTAATGGATACTGATCTTCACCAAATAATCCGCTCCAACCAAAGTTTATCAGAAGAGCATTGTCAGTATTTCCTTTATCAGATTCTCAGAGGACTGAAATACATACATTCGGCAAATGTCATTCATCGAGACTTGAAACCGAGCAATCTATTGCTTAATGCAAACTGTGATCTTAAAATATGTGACTTTGGTCTTGCTCGACCAACTTCTGAAAATGAATGTATGACGGAATATGTTGTGACAAGATGGTACAGAGCACCTGAGCTTCTTTTGAACTCTGATTATACAGCTGCAATCGATATATGGTCTGTTGGTTGCATCTTTCTGGAGCTTATGAATAGAAGGCCTTTATTTCCAGGCAGGGATCATGTGCATCAGATGAGATTATTGACTGAGCTTCTTGGCACACCAAGCGAGTCAGATCTTGGTTTTATCCGAAATGAAGACTCGAAAAGATATCTTCGACAGCTACCTCCACATCCTCGTCAGCCATTAGCAACGGTTTTTCCACATGTTCATCCGTTAGCAATTGATCTTGTGGATAAAATGTTGACATTTGACCCAACAAAGAGAATTACCGTTGAAGAAGCGTTAGCACATCCATACCTAGAAAGATTGCATGACATAGCCGATGAGCCGGTTTGCTCAGAGCCATTCTCGTTTGAGTTTGAGCAACAATACTTAGACGAAGAACAGATGAAGGAGATGATTTACAGAGAATCATTGGCACTCAATCCAGAATTTGCATGA

Coding sequence (CDS)

ATGGTTGCGGTTAAGAAGATTGCTAACGCGTTTGATAACCATATGGATGCGAAGAGGACGCTCCGTGAGATTAAGCTTCTACGCCATTTGGATCATGAAAATGTAATAGGCATAAGAGATGTGATTCCTCCACCTTTACGGAGAGAATTCAATGATGTCTACATTTCGACTGAACTAATGGATACTGATCTTCACCAAATAATCCGCTCCAACCAAAGTTTATCAGAAGAGCATTGTCAGTATTTCCTTTATCAGATTCTCAGAGGACTGAAATACATACATTCGGCAAATGTCATTCATCGAGACTTGAAACCGAGCAATCTATTGCTTAATGCAAACTGTGATCTTAAAATATGTGACTTTGGTCTTGCTCGACCAACTTCTGAAAATGAATGTATGACGGAATATGTTGTGACAAGATGGTACAGAGCACCTGAGCTTCTTTTGAACTCTGATTATACAGCTGCAATCGATATATGGTCTGTTGGTTGCATCTTTCTGGAGCTTATGAATAGAAGGCCTTTATTTCCAGGCAGGGATCATGTGCATCAGATGAGATTATTGACTGAGCTTCTTGGCACACCAAGCGAGTCAGATCTTGGTTTTATCCGAAATGAAGACTCGAAAAGATATCTTCGACAGCTACCTCCACATCCTCGTCAGCCATTAGCAACGGTTTTTCCACATGTTCATCCGTTAGCAATTGATCTTGTGGATAAAATGTTGACATTTGACCCAACAAAGAGAATTACCGTTGAAGAAGCGTTAGCACATCCATACCTAGAAAGATTGCATGACATAGCCGATGAGCCGGTTTGCTCAGAGCCATTCTCGTTTGAGTTTGAGCAACAATACTTAGACGAAGAACAGATGAAGGAGATGATTTACAGAGAATCATTGGCACTCAATCCAGAATTTGCATGA

Protein sequence

MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELMDTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICDFGLARPTSENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFPGRDHVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDKMLTFDPTKRITVEEALAHPYLERLHDIADEPVCSEPFSFEFEQQYLDEEQMKEMIYRESLALNPEFA*

Homology

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BLAST of Chy12G207220.1 vs. ExPASy Swiss-Prot
Match: Q39023 (Mitogen-activated protein kinase 3 OS=Arabidopsis thaliana OX=3702 GN=MPK3 PE=1 SV=2)

HSP 1 Score: 548.1 bits (1411), Expect = 6.2e-155
Identity = 259/307 (84.36%), Postives = 289/307 (94.14%), Query Frame = 0


Query: 1   MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELM 60
           +VA+KKIANAFDNHMDAKRTLREIKLLRHLDHEN+I IRDV+PPPLRR+F+DVYISTELM
Sbjct: 63  LVAMKKIANAFDNHMDAKRTLREIKLLRHLDHENIIAIRDVVPPPLRRQFSDVYISTELM 122

Query: 61  DTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD 120
           DTDLHQIIRSNQSLSEEHCQYFLYQ+LRGLKYIHSAN+IHRDLKPSNLLLNANCDLKICD
Sbjct: 123 DTDLHQIIRSNQSLSEEHCQYFLYQLLRGLKYIHSANIIHRDLKPSNLLLNANCDLKICD 182

Query: 121 FGLARPTSENECMTEYVVTRWYRAPELLLN-SDYTAAIDIWSVGCIFLELMNRRPLFPGR 180
           FGLARPTSEN+ MTEYVVTRWYRAPELLLN SDYTAAID+WSVGCIF+ELMNR+PLFPG+
Sbjct: 183 FGLARPTSENDFMTEYVVTRWYRAPELLLNSSDYTAAIDVWSVGCIFMELMNRKPLFPGK 242

Query: 181 DHVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVD 240
           DHVHQMRLLTELLGTP+ESDLGF  NED+KRY+RQLP  PRQPLA +F HV+P+AIDLVD
Sbjct: 243 DHVHQMRLLTELLGTPTESDLGFTHNEDAKRYIRQLPNFPRQPLAKLFSHVNPMAIDLVD 302

Query: 241 KMLTFDPTKRITVEEALAHPYLERLHDIADEPVCSEPFSFEFEQQYLDEEQMKEMIYRES 300
           +MLTFDP +RITVE+AL H YL +LHD  DEP+C +PFSFEFEQQ LDEEQ+KEMIY+E+
Sbjct: 303 RMLTFDPNRRITVEQALNHQYLAKLHDPNDEPICQKPFSFEFEQQPLDEEQIKEMIYQEA 362

Query: 301 LALNPEF 307
           +ALNP +
Sbjct: 363 IALNPTY 369

[Close]

BLAST of Chy12G207220.1 vs. ExPASy Swiss-Prot
Match: Q07176 (Mitogen-activated protein kinase homolog MMK1 OS=Medicago sativa OX=3879 GN=MMK1 PE=1 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 1.1e-146
Identity = 250/306 (81.70%), Postives = 277/306 (90.52%), Query Frame = 0


Query: 2   VAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELMD 61
           VAVKKIANAFDN +DAKRTLREIKLLRH+DHENV+ IRD++PPP R  FNDVYI+ ELMD
Sbjct: 81  VAVKKIANAFDNKIDAKRTLREIKLLRHMDHENVVAIRDIVPPPQREVFNDVYIAYELMD 140

Query: 62  TDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICDF 121
           TDLHQIIRSNQ+LSEEHCQYFLYQILRGLKYIHSANV+HRDLKPSNLLLNANCDLKICDF
Sbjct: 141 TDLHQIIRSNQALSEEHCQYFLYQILRGLKYIHSANVLHRDLKPSNLLLNANCDLKICDF 200

Query: 122 GLARPTSENECMTEYVVTRWYRAPELLLN-SDYTAAIDIWSVGCIFLELMNRRPLFPGRD 181
           GLAR TSE + MTEYVVTRWYRAPELLLN SDYTAAID+WSVGCIF+ELM+R+PLFPGRD
Sbjct: 201 GLARVTSETDFMTEYVVTRWYRAPELLLNSSDYTAAIDVWSVGCIFMELMDRKPLFPGRD 260

Query: 182 HVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK 241
           HVHQ+RLL EL+GTPSE DLGF+ NE++KRY+RQLPP+ RQ     FPHVHP AIDLV+K
Sbjct: 261 HVHQLRLLMELIGTPSEDDLGFL-NENAKRYIRQLPPYRRQSFQEKFPHVHPEAIDLVEK 320

Query: 242 MLTFDPTKRITVEEALAHPYLERLHDIADEPVCSEPFSFEFEQQYLDEEQMKEMIYRESL 301
           MLTFDP KRITVE+ALAHPYL  LHDI+DEPVC  PFSF+FEQ  L EEQMKE+IYRE+L
Sbjct: 321 MLTFDPRKRITVEDALAHPYLTSLHDISDEPVCMTPFSFDFEQHALTEEQMKELIYREAL 380

Query: 302 ALNPEF 307
           A NPE+
Sbjct: 381 AFNPEY 385

[Close]

BLAST of Chy12G207220.1 vs. ExPASy Swiss-Prot
Match: A2XFC8 (Mitogen-activated protein kinase 5 OS=Oryza sativa subsp. indica OX=39946 GN=MPK5 PE=1 SV=2)

HSP 1 Score: 517.3 bits (1331), Expect = 1.2e-145
Identity = 238/305 (78.03%), Postives = 284/305 (93.11%), Query Frame = 0


Query: 1   MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELM 60
           MVA+KKIANAF+N MDAKRTLREIKLLRHLDHEN+IGIRDVIPPP+ + FNDVYI+TELM
Sbjct: 61  MVAIKKIANAFNNDMDAKRTLREIKLLRHLDHENIIGIRDVIPPPIPQAFNDVYIATELM 120

Query: 61  DTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD 120
           DTDLH IIRSNQ LSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD
Sbjct: 121 DTDLHHIIRSNQELSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD 180

Query: 121 FGLARPTSENECMTEYVVTRWYRAPELLLNS-DYTAAIDIWSVGCIFLELMNRRPLFPGR 180
           FGLARP+SE++ MTEYVVTRWYRAPELLLNS DY+AAID+WSVGCIF+EL+NR+PLFPGR
Sbjct: 181 FGLARPSSESDMMTEYVVTRWYRAPELLLNSTDYSAAIDVWSVGCIFMELINRQPLFPGR 240

Query: 181 DHVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVD 240
           DH+HQMRL+TE++GTP++ +LGFIRNED+++Y+R LP +PR+  A++FP V P A+DL++
Sbjct: 241 DHMHQMRLITEVIGTPTDDELGFIRNEDARKYMRHLPQYPRRTFASMFPRVQPAALDLIE 300

Query: 241 KMLTFDPTKRITVEEALAHPYLERLHDIADEPVCSEPFSFEFEQQYLDEEQMKEMIYRES 300
           +MLTF+P +RITVEEAL HPYLERLHDIADEP+C EPFSF+FEQ+ L+E+QMK++I+ E+
Sbjct: 301 RMLTFNPLQRITVEEALDHPYLERLHDIADEPICLEPFSFDFEQKALNEDQMKQLIFNEA 360

Query: 301 LALNP 305
           + +NP
Sbjct: 361 IEMNP 365

[Close]

BLAST of Chy12G207220.1 vs. ExPASy Swiss-Prot
Match: Q10N20 (Mitogen-activated protein kinase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=MPK5 PE=1 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 1.2e-145
Identity = 238/305 (78.03%), Postives = 284/305 (93.11%), Query Frame = 0


Query: 1   MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELM 60
           MVA+KKIANAF+N MDAKRTLREIKLLRHLDHEN+IGIRDVIPPP+ + FNDVYI+TELM
Sbjct: 61  MVAIKKIANAFNNDMDAKRTLREIKLLRHLDHENIIGIRDVIPPPIPQAFNDVYIATELM 120

Query: 61  DTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD 120
           DTDLH IIRSNQ LSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD
Sbjct: 121 DTDLHHIIRSNQELSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD 180

Query: 121 FGLARPTSENECMTEYVVTRWYRAPELLLNS-DYTAAIDIWSVGCIFLELMNRRPLFPGR 180
           FGLARP+SE++ MTEYVVTRWYRAPELLLNS DY+AAID+WSVGCIF+EL+NR+PLFPGR
Sbjct: 181 FGLARPSSESDMMTEYVVTRWYRAPELLLNSTDYSAAIDVWSVGCIFMELINRQPLFPGR 240

Query: 181 DHVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVD 240
           DH+HQMRL+TE++GTP++ +LGFIRNED+++Y+R LP +PR+  A++FP V P A+DL++
Sbjct: 241 DHMHQMRLITEVIGTPTDDELGFIRNEDARKYMRHLPQYPRRTFASMFPRVQPAALDLIE 300

Query: 241 KMLTFDPTKRITVEEALAHPYLERLHDIADEPVCSEPFSFEFEQQYLDEEQMKEMIYRES 300
           +MLTF+P +RITVEEAL HPYLERLHDIADEP+C EPFSF+FEQ+ L+E+QMK++I+ E+
Sbjct: 301 RMLTFNPLQRITVEEALDHPYLERLHDIADEPICLEPFSFDFEQKALNEDQMKQLIFNEA 360

Query: 301 LALNP 305
           + +NP
Sbjct: 361 IEMNP 365

[Close]

BLAST of Chy12G207220.1 vs. ExPASy Swiss-Prot
Match: Q06060 (Mitogen-activated protein kinase homolog D5 OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 7.7e-145
Identity = 247/306 (80.72%), Postives = 277/306 (90.52%), Query Frame = 0


Query: 2   VAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELMD 61
           VAVKKIANAFDN +DAKRTLREIKL+RH+DHENV+ IRD++PPP R  FNDVYI+ ELMD
Sbjct: 88  VAVKKIANAFDNKIDAKRTLREIKLVRHMDHENVVAIRDIVPPPQREVFNDVYIAYELMD 147

Query: 62  TDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICDF 121
           TDLHQIIRSNQ+LSEEHCQYFLYQILRGLKYIHSANV+HRDLKPSNLLLNANCDLKICDF
Sbjct: 148 TDLHQIIRSNQALSEEHCQYFLYQILRGLKYIHSANVLHRDLKPSNLLLNANCDLKICDF 207

Query: 122 GLARPTSENECMTEYVVTRWYRAPELLLN-SDYTAAIDIWSVGCIFLELMNRRPLFPGRD 181
           GLAR TSE + MTEYVVTRWYRAPELLLN SDYTAAID+WSVGCIF+ELM+R+PLFPGRD
Sbjct: 208 GLARVTSETDFMTEYVVTRWYRAPELLLNSSDYTAAIDVWSVGCIFMELMDRKPLFPGRD 267

Query: 182 HVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK 241
           HVHQ+RLL EL+GTPSE+DLGF+ NE++KRY+RQLP + RQ     FPHVHP AIDLV+K
Sbjct: 268 HVHQLRLLMELIGTPSEADLGFL-NENAKRYIRQLPLYRRQSFQEKFPHVHPEAIDLVEK 327

Query: 242 MLTFDPTKRITVEEALAHPYLERLHDIADEPVCSEPFSFEFEQQYLDEEQMKEMIYRESL 301
           MLTFDP +RITVE ALAHPYL  LHDI+DEPVC+ PFSF+FEQ  L EEQMKE+IYRE+L
Sbjct: 328 MLTFDPRQRITVENALAHPYLTSLHDISDEPVCTTPFSFDFEQHALTEEQMKELIYREAL 387

Query: 302 ALNPEF 307
           A NPE+
Sbjct: 388 AFNPEY 392

[Close]

BLAST of Chy12G207220.1 vs. ExPASy TrEMBL
Match: Q0R4I2 (Mitogen-activated protein kinase OS=Cucumis sativus OX=3659 GN=TIPK PE=2 SV=1)

HSP 1 Score: 630.9 bits (1626), Expect = 2.7e-177
Identity = 306/307 (99.67%), Postives = 307/307 (100.00%), Query Frame = 0


Query: 1   MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELM 60
           MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELM
Sbjct: 64  MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELM 123

Query: 61  DTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD 120
           DTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD
Sbjct: 124 DTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD 183

Query: 121 FGLARPTSENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFPGRD 180
           FGLARPTSENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFPGRD
Sbjct: 184 FGLARPTSENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFPGRD 243

Query: 181 HVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK 240
           HVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK
Sbjct: 244 HVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK 303

Query: 241 MLTFDPTKRITVEEALAHPYLERLHDIADEPVCSEPFSFEFEQQYLDEEQMKEMIYRESL 300
           MLTFDPTKRITVEEALAHPYLERLHDIADEPVCSEPFSFEFEQQYLDEEQMKEMIYRE+L
Sbjct: 304 MLTFDPTKRITVEEALAHPYLERLHDIADEPVCSEPFSFEFEQQYLDEEQMKEMIYREAL 363

Query: 301 ALNPEFA 308
           ALNPEFA
Sbjct: 364 ALNPEFA 370

[Close]

BLAST of Chy12G207220.1 vs. ExPASy TrEMBL
Match: A0A5A7T8I9 (Mitogen-activated protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold918G00050 PE=3 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 2.3e-176
Identity = 304/307 (99.02%), Postives = 306/307 (99.67%), Query Frame = 0


Query: 1   MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELM 60
           MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELM
Sbjct: 100 MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELM 159

Query: 61  DTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD 120
           DTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD
Sbjct: 160 DTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD 219

Query: 121 FGLARPTSENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFPGRD 180
           FGLARPTSENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFPGRD
Sbjct: 220 FGLARPTSENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFPGRD 279

Query: 181 HVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK 240
           HVHQMRLLTELLGTP+ESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK
Sbjct: 280 HVHQMRLLTELLGTPTESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK 339

Query: 241 MLTFDPTKRITVEEALAHPYLERLHDIADEPVCSEPFSFEFEQQYLDEEQMKEMIYRESL 300
           MLTFDPTKRITVEEALAHPYLERLHD ADEPVCSEPFSFEFEQQYLDEEQMKEMIYRE+L
Sbjct: 340 MLTFDPTKRITVEEALAHPYLERLHDTADEPVCSEPFSFEFEQQYLDEEQMKEMIYREAL 399

Query: 301 ALNPEFA 308
           ALNPEFA
Sbjct: 400 ALNPEFA 406

[Close]

BLAST of Chy12G207220.1 vs. ExPASy TrEMBL
Match: A0A5D3BXD1 (Mitogen-activated protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold968G00210 PE=3 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 2.3e-176
Identity = 304/307 (99.02%), Postives = 306/307 (99.67%), Query Frame = 0


Query: 1   MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELM 60
           MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELM
Sbjct: 86  MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELM 145

Query: 61  DTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD 120
           DTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD
Sbjct: 146 DTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD 205

Query: 121 FGLARPTSENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFPGRD 180
           FGLARPTSENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFPGRD
Sbjct: 206 FGLARPTSENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFPGRD 265

Query: 181 HVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK 240
           HVHQMRLLTELLGTP+ESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK
Sbjct: 266 HVHQMRLLTELLGTPTESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK 325

Query: 241 MLTFDPTKRITVEEALAHPYLERLHDIADEPVCSEPFSFEFEQQYLDEEQMKEMIYRESL 300
           MLTFDPTKRITVEEALAHPYLERLHD ADEPVCSEPFSFEFEQQYLDEEQMKEMIYRE+L
Sbjct: 326 MLTFDPTKRITVEEALAHPYLERLHDTADEPVCSEPFSFEFEQQYLDEEQMKEMIYREAL 385

Query: 301 ALNPEFA 308
           ALNPEFA
Sbjct: 386 ALNPEFA 392

[Close]

BLAST of Chy12G207220.1 vs. ExPASy TrEMBL
Match: F8ULD9 (Mitogen-activated protein kinase OS=Cucumis melo OX=3656 GN=LOC103497219 PE=2 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 1.1e-175
Identity = 303/307 (98.70%), Postives = 305/307 (99.35%), Query Frame = 0


Query: 1   MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELM 60
           MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYI TELM
Sbjct: 64  MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYILTELM 123

Query: 61  DTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD 120
           DTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD
Sbjct: 124 DTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD 183

Query: 121 FGLARPTSENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFPGRD 180
           FGLARPTSENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFPGRD
Sbjct: 184 FGLARPTSENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFPGRD 243

Query: 181 HVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK 240
           HVHQMRLLTELLGTP+ESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK
Sbjct: 244 HVHQMRLLTELLGTPTESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK 303

Query: 241 MLTFDPTKRITVEEALAHPYLERLHDIADEPVCSEPFSFEFEQQYLDEEQMKEMIYRESL 300
           MLTFDPTKRITVEEALAHPYLERLHD ADEPVCSEPFSFEFEQQYLDEEQMKEMIYRE+L
Sbjct: 304 MLTFDPTKRITVEEALAHPYLERLHDTADEPVCSEPFSFEFEQQYLDEEQMKEMIYREAL 363

Query: 301 ALNPEFA 308
           ALNPEFA
Sbjct: 364 ALNPEFA 370

[Close]

BLAST of Chy12G207220.1 vs. ExPASy TrEMBL
Match: A0A6J1KFE1 (Mitogen-activated protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111492741 PE=3 SV=1)

HSP 1 Score: 618.2 bits (1593), Expect = 1.8e-173
Identity = 298/307 (97.07%), Postives = 305/307 (99.35%), Query Frame = 0


Query: 1   MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELM 60
           MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREF+DVYISTELM
Sbjct: 64  MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFSDVYISTELM 123

Query: 61  DTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD 120
           DTDLHQIIRSNQ+LSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD
Sbjct: 124 DTDLHQIIRSNQTLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD 183

Query: 121 FGLARPTSENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFPGRD 180
           FGLARPTSENE MTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCI+LELMNRRPLFPGRD
Sbjct: 184 FGLARPTSENESMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIYLELMNRRPLFPGRD 243

Query: 181 HVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK 240
           HVHQMRLLTELLGTP+ESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK
Sbjct: 244 HVHQMRLLTELLGTPTESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK 303

Query: 241 MLTFDPTKRITVEEALAHPYLERLHDIADEPVCSEPFSFEFEQQYLDEEQMKEMIYRESL 300
           MLTFDPT+RITVEEALAHPYLERLHD+ADEPVC EPFSFEFEQQYLDEEQMKEMIYRE+L
Sbjct: 304 MLTFDPTRRITVEEALAHPYLERLHDVADEPVCPEPFSFEFEQQYLDEEQMKEMIYREAL 363

Query: 301 ALNPEFA 308
           ALNPEFA
Sbjct: 364 ALNPEFA 370

[Close]

BLAST of Chy12G207220.1 vs. NCBI nr
Match: NP_001267653.1 (mitogen-activated protein kinase 3-like [Cucumis sativus] >AAZ57337.1 Trichoderma-induced protein kinase [Cucumis sativus] >ABG89857.1 NO3-stress-induced mitogen activated protein kinase [Cucumis sativus] >KGN65657.1 hypothetical protein Csa_020125 [Cucumis sativus])

HSP 1 Score: 630 bits (1624), Expect = 7.40e-227
Identity = 306/307 (99.67%), Postives = 307/307 (100.00%), Query Frame = 0


Query: 1   MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELM 60
           MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELM
Sbjct: 64  MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELM 123

Query: 61  DTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD 120
           DTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD
Sbjct: 124 DTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD 183

Query: 121 FGLARPTSENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFPGRD 180
           FGLARPTSENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFPGRD
Sbjct: 184 FGLARPTSENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFPGRD 243

Query: 181 HVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK 240
           HVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK
Sbjct: 244 HVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK 303

Query: 241 MLTFDPTKRITVEEALAHPYLERLHDIADEPVCSEPFSFEFEQQYLDEEQMKEMIYRESL 300
           MLTFDPTKRITVEEALAHPYLERLHDIADEPVCSEPFSFEFEQQYLDEEQMKEMIYRE+L
Sbjct: 304 MLTFDPTKRITVEEALAHPYLERLHDIADEPVCSEPFSFEFEQQYLDEEQMKEMIYREAL 363

Query: 301 ALNPEFA 307
           ALNPEFA
Sbjct: 364 ALNPEFA 370

[Close]

BLAST of Chy12G207220.1 vs. NCBI nr
Match: XP_038895412.1 (mitogen-activated protein kinase 3 [Benincasa hispida])

HSP 1 Score: 627 bits (1616), Expect = 1.23e-225
Identity = 304/307 (99.02%), Postives = 306/307 (99.67%), Query Frame = 0


Query: 1   MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELM 60
           MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELM
Sbjct: 64  MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELM 123

Query: 61  DTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD 120
           DTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD
Sbjct: 124 DTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD 183

Query: 121 FGLARPTSENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFPGRD 180
           FGLARPTSENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFPGRD
Sbjct: 184 FGLARPTSENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFPGRD 243

Query: 181 HVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK 240
           HVHQMRLLTELLGTP+ESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK
Sbjct: 244 HVHQMRLLTELLGTPTESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK 303

Query: 241 MLTFDPTKRITVEEALAHPYLERLHDIADEPVCSEPFSFEFEQQYLDEEQMKEMIYRESL 300
           MLTFDPTKRITVEEALAHPYLERLHDIADEPVC EPFSFEFEQQYLDEEQMKEMIYRE+L
Sbjct: 304 MLTFDPTKRITVEEALAHPYLERLHDIADEPVCPEPFSFEFEQQYLDEEQMKEMIYREAL 363

Query: 301 ALNPEFA 307
           ALNPEFA
Sbjct: 364 ALNPEFA 370

[Close]

BLAST of Chy12G207220.1 vs. NCBI nr
Match: TYK02886.1 (mitogen-activated protein kinase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 627 bits (1616), Expect = 2.76e-225
Identity = 304/307 (99.02%), Postives = 306/307 (99.67%), Query Frame = 0


Query: 1   MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELM 60
           MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELM
Sbjct: 86  MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELM 145

Query: 61  DTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD 120
           DTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD
Sbjct: 146 DTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD 205

Query: 121 FGLARPTSENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFPGRD 180
           FGLARPTSENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFPGRD
Sbjct: 206 FGLARPTSENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFPGRD 265

Query: 181 HVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK 240
           HVHQMRLLTELLGTP+ESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK
Sbjct: 266 HVHQMRLLTELLGTPTESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK 325

Query: 241 MLTFDPTKRITVEEALAHPYLERLHDIADEPVCSEPFSFEFEQQYLDEEQMKEMIYRESL 300
           MLTFDPTKRITVEEALAHPYLERLHD ADEPVCSEPFSFEFEQQYLDEEQMKEMIYRE+L
Sbjct: 326 MLTFDPTKRITVEEALAHPYLERLHDTADEPVCSEPFSFEFEQQYLDEEQMKEMIYREAL 385

Query: 301 ALNPEFA 307
           ALNPEFA
Sbjct: 386 ALNPEFA 392

[Close]

BLAST of Chy12G207220.1 vs. NCBI nr
Match: KAA0037795.1 (mitogen-activated protein kinase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 627 bits (1616), Expect = 4.62e-225
Identity = 304/307 (99.02%), Postives = 306/307 (99.67%), Query Frame = 0


Query: 1   MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELM 60
           MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELM
Sbjct: 100 MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELM 159

Query: 61  DTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD 120
           DTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD
Sbjct: 160 DTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD 219

Query: 121 FGLARPTSENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFPGRD 180
           FGLARPTSENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFPGRD
Sbjct: 220 FGLARPTSENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFPGRD 279

Query: 181 HVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK 240
           HVHQMRLLTELLGTP+ESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK
Sbjct: 280 HVHQMRLLTELLGTPTESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK 339

Query: 241 MLTFDPTKRITVEEALAHPYLERLHDIADEPVCSEPFSFEFEQQYLDEEQMKEMIYRESL 300
           MLTFDPTKRITVEEALAHPYLERLHD ADEPVCSEPFSFEFEQQYLDEEQMKEMIYRE+L
Sbjct: 340 MLTFDPTKRITVEEALAHPYLERLHDTADEPVCSEPFSFEFEQQYLDEEQMKEMIYREAL 399

Query: 301 ALNPEFA 307
           ALNPEFA
Sbjct: 400 ALNPEFA 406

[Close]

BLAST of Chy12G207220.1 vs. NCBI nr
Match: NP_001284376.1 (mitogen-activated protein kinase 3 [Cucumis melo] >AEG89601.1 mitogen-activated protein [Cucumis melo])

HSP 1 Score: 624 bits (1610), Expect = 1.01e-224
Identity = 303/307 (98.70%), Postives = 305/307 (99.35%), Query Frame = 0


Query: 1   MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELM 60
           MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYI TELM
Sbjct: 64  MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYILTELM 123

Query: 61  DTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD 120
           DTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD
Sbjct: 124 DTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD 183

Query: 121 FGLARPTSENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFPGRD 180
           FGLARPTSENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFPGRD
Sbjct: 184 FGLARPTSENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFPGRD 243

Query: 181 HVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK 240
           HVHQMRLLTELLGTP+ESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK
Sbjct: 244 HVHQMRLLTELLGTPTESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK 303

Query: 241 MLTFDPTKRITVEEALAHPYLERLHDIADEPVCSEPFSFEFEQQYLDEEQMKEMIYRESL 300
           MLTFDPTKRITVEEALAHPYLERLHD ADEPVCSEPFSFEFEQQYLDEEQMKEMIYRE+L
Sbjct: 304 MLTFDPTKRITVEEALAHPYLERLHDTADEPVCSEPFSFEFEQQYLDEEQMKEMIYREAL 363

Query: 301 ALNPEFA 307
           ALNPEFA
Sbjct: 364 ALNPEFA 370

[Close]

BLAST of Chy12G207220.1 vs. TAIR 10
Match: AT3G45640.1 (mitogen-activated protein kinase 3 )

HSP 1 Score: 548.1 bits (1411), Expect = 4.4e-156
Identity = 259/307 (84.36%), Postives = 289/307 (94.14%), Query Frame = 0


Query: 1   MVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELM 60
           +VA+KKIANAFDNHMDAKRTLREIKLLRHLDHEN+I IRDV+PPPLRR+F+DVYISTELM
Sbjct: 63  LVAMKKIANAFDNHMDAKRTLREIKLLRHLDHENIIAIRDVVPPPLRRQFSDVYISTELM 122

Query: 61  DTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICD 120
           DTDLHQIIRSNQSLSEEHCQYFLYQ+LRGLKYIHSAN+IHRDLKPSNLLLNANCDLKICD
Sbjct: 123 DTDLHQIIRSNQSLSEEHCQYFLYQLLRGLKYIHSANIIHRDLKPSNLLLNANCDLKICD 182

Query: 121 FGLARPTSENECMTEYVVTRWYRAPELLLN-SDYTAAIDIWSVGCIFLELMNRRPLFPGR 180
           FGLARPTSEN+ MTEYVVTRWYRAPELLLN SDYTAAID+WSVGCIF+ELMNR+PLFPG+
Sbjct: 183 FGLARPTSENDFMTEYVVTRWYRAPELLLNSSDYTAAIDVWSVGCIFMELMNRKPLFPGK 242

Query: 181 DHVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVD 240
           DHVHQMRLLTELLGTP+ESDLGF  NED+KRY+RQLP  PRQPLA +F HV+P+AIDLVD
Sbjct: 243 DHVHQMRLLTELLGTPTESDLGFTHNEDAKRYIRQLPNFPRQPLAKLFSHVNPMAIDLVD 302

Query: 241 KMLTFDPTKRITVEEALAHPYLERLHDIADEPVCSEPFSFEFEQQYLDEEQMKEMIYRES 300
           +MLTFDP +RITVE+AL H YL +LHD  DEP+C +PFSFEFEQQ LDEEQ+KEMIY+E+
Sbjct: 303 RMLTFDPNRRITVEQALNHQYLAKLHDPNDEPICQKPFSFEFEQQPLDEEQIKEMIYQEA 362

Query: 301 LALNPEF 307
           +ALNP +
Sbjct: 363 IALNPTY 369

[Close]

BLAST of Chy12G207220.1 vs. TAIR 10
Match: AT2G43790.1 (MAP kinase 6 )

HSP 1 Score: 513.1 bits (1320), Expect = 1.6e-145
Identity = 243/306 (79.41%), Postives = 278/306 (90.85%), Query Frame = 0


Query: 2   VAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELMD 61
           VA+KKIANAFDN +DAKRTLREIKLLRH+DHEN++ IRD+IPPPLR  FNDVYI+ ELMD
Sbjct: 89  VAIKKIANAFDNKIDAKRTLREIKLLRHMDHENIVAIRDIIPPPLRNAFNDVYIAYELMD 148

Query: 62  TDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICDF 121
           TDLHQIIRSNQ+LSEEHCQYFLYQILRGLKYIHSANV+HRDLKPSNLLLNANCDLKICDF
Sbjct: 149 TDLHQIIRSNQALSEEHCQYFLYQILRGLKYIHSANVLHRDLKPSNLLLNANCDLKICDF 208

Query: 122 GLARPTSENECMTEYVVTRWYRAPELLLN-SDYTAAIDIWSVGCIFLELMNRRPLFPGRD 181
           GLAR TSE++ MTEYVVTRWYRAPELLLN SDYTAAID+WSVGCIF+ELM+R+PLFPGRD
Sbjct: 209 GLARVTSESDFMTEYVVTRWYRAPELLLNSSDYTAAIDVWSVGCIFMELMDRKPLFPGRD 268

Query: 182 HVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK 241
           HVHQ+RLL EL+GTPSE +L F+ NE++KRY+RQLPP+PRQ +   FP VHPLAIDL++K
Sbjct: 269 HVHQLRLLMELIGTPSEEELEFL-NENAKRYIRQLPPYPRQSITDKFPTVHPLAIDLIEK 328

Query: 242 MLTFDPTKRITVEEALAHPYLERLHDIADEPVCSEPFSFEFEQQYLDEEQMKEMIYRESL 301
           MLTFDP +RITV +ALAHPYL  LHDI+DEP C+ PF+F+FE   L EEQMKE+IYRE+L
Sbjct: 329 MLTFDPRRRITVLDALAHPYLNSLHDISDEPECTIPFNFDFENHALSEEQMKELIYREAL 388

Query: 302 ALNPEF 307
           A NPE+
Sbjct: 389 AFNPEY 393

[Close]

BLAST of Chy12G207220.1 vs. TAIR 10
Match: AT4G01370.1 (MAP kinase 4 )

HSP 1 Score: 454.9 bits (1169), Expect = 5.1e-128
Identity = 212/305 (69.51%), Postives = 261/305 (85.57%), Query Frame = 0


Query: 2   VAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELMD 61
           VA+KKI NAFDN +DAKRTLREIKLL+H+DHENVI ++D+I PP R  FNDVYI  ELMD
Sbjct: 69  VAIKKIGNAFDNIIDAKRTLREIKLLKHMDHENVIAVKDIIKPPQRENFNDVYIVYELMD 128

Query: 62  TDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICDF 121
           TDLHQIIRSNQ L+++HC++FLYQ+LRGLKY+HSANV+HRDLKPSNLLLNANCDLK+ DF
Sbjct: 129 TDLHQIIRSNQPLTDDHCRFFLYQLLRGLKYVHSANVLHRDLKPSNLLLNANCDLKLGDF 188

Query: 122 GLARPTSENECMTEYVVTRWYRAPELLLN-SDYTAAIDIWSVGCIFLELMNRRPLFPGRD 181
           GLAR  SE + MTEYVVTRWYRAPELLLN S+YTAAIDIWSVGCI  E M R PLFPG+D
Sbjct: 189 GLARTKSETDFMTEYVVTRWYRAPELLLNCSEYTAAIDIWSVGCILGETMTREPLFPGKD 248

Query: 182 HVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK 241
           +VHQ+RL+TEL+G+P +S LGF+R+++++RY+RQLP +PRQ  A  FP++   A+DL++K
Sbjct: 249 YVHQLRLITELIGSPDDSSLGFLRSDNARRYVRQLPQYPRQNFAARFPNMSAGAVDLLEK 308

Query: 242 MLTFDPTKRITVEEALAHPYLERLHDIADEPVCSEPFSFEFEQQYLDEEQMKEMIYRESL 301
           ML FDP++RITV+EAL HPYL  LHDI +EPVC  PF+F+FEQ  L EE +KE+IYRE++
Sbjct: 309 MLVFDPSRRITVDEALCHPYLAPLHDINEEPVCVRPFNFDFEQPTLTEENIKELIYRETV 368

Query: 302 ALNPE 306
             NP+
Sbjct: 369 KFNPQ 373

[Close]

BLAST of Chy12G207220.1 vs. TAIR 10
Match: AT4G11330.1 (MAP kinase 5 )

HSP 1 Score: 441.4 bits (1134), Expect = 5.9e-124
Identity = 207/304 (68.09%), Postives = 255/304 (83.88%), Query Frame = 0


Query: 2   VAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELMD 61
           +A+KKI  AFDN +DAKRTLREIKLLRHL+HENV+ I+D+I PP + +F DVYI  ELMD
Sbjct: 69  IAIKKIGKAFDNKVDAKRTLREIKLLRHLEHENVVVIKDIIRPPKKEDFVDVYIVFELMD 128

Query: 62  TDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICDF 121
           TDLHQIIRSNQSL+++HCQYFLYQILRGLKYIHSANV+HRDLKPSNLLLN+NCDLKI DF
Sbjct: 129 TDLHQIIRSNQSLNDDHCQYFLYQILRGLKYIHSANVLHRDLKPSNLLLNSNCDLKITDF 188

Query: 122 GLARPTSENECMTEYVVTRWYRAPELLLN-SDYTAAIDIWSVGCIFLELMNRRPLFPGRD 181
           GLAR TSE E MTEYVVTRWYRAPELLLN S+YT+AID+WSVGCIF E+M R PLFPG+D
Sbjct: 189 GLARTTSETEYMTEYVVTRWYRAPELLLNSSEYTSAIDVWSVGCIFAEIMTREPLFPGKD 248

Query: 182 HVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK 241
           +VHQ++L+TEL+G+P  + L F+R+ ++++Y+++LP  PRQ  +  FP ++  AIDL++K
Sbjct: 249 YVHQLKLITELIGSPDGASLEFLRSANARKYVKELPKFPRQNFSARFPSMNSTAIDLLEK 308

Query: 242 MLTFDPTKRITVEEALAHPYLERLHDIADEPVCSEPFSFEFEQQYLDEEQMKEMIYRESL 301
           ML FDP KRITVEEAL +PYL  LHD+ DEPVCS  FSF FE     EE++KE+++ ES+
Sbjct: 309 MLVFDPVKRITVEEALCYPYLSALHDLNDEPVCSNHFSFHFEDPSSTEEEIKELVWLESV 368

Query: 302 ALNP 305
             NP
Sbjct: 369 KFNP 372

[Close]

BLAST of Chy12G207220.1 vs. TAIR 10
Match: AT1G07880.2 (Protein kinase superfamily protein )

HSP 1 Score: 435.6 bits (1119), Expect = 3.2e-122
Identity = 202/300 (67.33%), Postives = 254/300 (84.67%), Query Frame = 0


Query: 2   VAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELMD 61
           VA+KKIANAFDN +DAKRTLREIKLL H+DH+NVI I+D+I  P +  F DVYI  ELMD
Sbjct: 59  VAIKKIANAFDNRVDAKRTLREIKLLSHMDHDNVIKIKDIIELPEKERFEDVYIVYELMD 118

Query: 62  TDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICDF 121
           TDLHQIIRS Q+L+++HCQYFLYQILRGLKYIHSANV+HRDLKPSNL+LN NCDLKICDF
Sbjct: 119 TDLHQIIRSTQTLTDDHCQYFLYQILRGLKYIHSANVLHRDLKPSNLVLNTNCDLKICDF 178

Query: 122 GLARPTSENECMTEYVVTRWYRAPELLLN-SDYTAAIDIWSVGCIFLELMNRRPLFPGRD 181
           GLAR ++E E MTEYVVTRWYRAPELLLN S+YT AIDIWSVGCIF+E++ R  LFPG+D
Sbjct: 179 GLARTSNETEIMTEYVVTRWYRAPELLLNSSEYTGAIDIWSVGCIFMEILRRETLFPGKD 238

Query: 182 HVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDK 241
           +V Q++L+TELLG+P +SDL F+R++++++Y++QLP   +Q     FP++ P+A+DL +K
Sbjct: 239 YVQQLKLITELLGSPDDSDLDFLRSDNARKYVKQLPHVQKQSFREKFPNISPMALDLAEK 298

Query: 242 MLTFDPTKRITVEEALAHPYLERLHDIADEPVCSEPFSFEFEQQYLDEEQMKEMIYRESL 301
           ML FDP+KRITV+EAL  PYL  LH+I +EP C  PFSF+FE+  LDE+ +KE+++RESL
Sbjct: 299 MLVFDPSKRITVDEALKQPYLASLHEINEEPTCPTPFSFDFEETALDEQDIKELVWRESL 358

The following BLAST results are available for this feature:

BLAST of Chy12G207220.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39023	6.2e-155	84.36	Mitogen-activated protein kinase 3 OS=Arabidopsis thaliana OX=3702 GN=MPK3 PE=1 ...	[more]
Q07176	1.1e-146	81.70	Mitogen-activated protein kinase homolog MMK1 OS=Medicago sativa OX=3879 GN=MMK1...	[more]
A2XFC8	1.2e-145	78.03	Mitogen-activated protein kinase 5 OS=Oryza sativa subsp. indica OX=39946 GN=MPK...	[more]
Q10N20	1.2e-145	78.03	Mitogen-activated protein kinase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=M...	[more]
Q06060	7.7e-145	80.72	Mitogen-activated protein kinase homolog D5 OS=Pisum sativum OX=3888 PE=2 SV=1	[more]

BLAST of Chy12G207220.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
Q0R4I2	2.7e-177	99.67	Mitogen-activated protein kinase OS=Cucumis sativus OX=3659 GN=TIPK PE=2 SV=1	[more]
A0A5A7T8I9	2.3e-176	99.02	Mitogen-activated protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A5D3BXD1	2.3e-176	99.02	Mitogen-activated protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
F8ULD9	1.1e-175	98.70	Mitogen-activated protein kinase OS=Cucumis melo OX=3656 GN=LOC103497219 PE=2 SV...	[more]
A0A6J1KFE1	1.8e-173	97.07	Mitogen-activated protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111492741 PE=...	[more]

BLAST of Chy12G207220.1 vs. NCBI nr


Match Name	E-value	Identity	Description
NP_001267653.1	7.40e-227	99.67	mitogen-activated protein kinase 3-like [Cucumis sativus] >AAZ57337.1 Trichoderm...	[more]
XP_038895412.1	1.23e-225	99.02	mitogen-activated protein kinase 3 [Benincasa hispida]	[more]
TYK02886.1	2.76e-225	99.02	mitogen-activated protein kinase 3 [Cucumis melo var. makuwa]	[more]
KAA0037795.1	4.62e-225	99.02	mitogen-activated protein kinase 3 [Cucumis melo var. makuwa]	[more]
NP_001284376.1	1.01e-224	98.70	mitogen-activated protein kinase 3 [Cucumis melo] >AEG89601.1 mitogen-activated ...	[more]

BLAST of Chy12G207220.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G45640.1	4.4e-156	84.36	mitogen-activated protein kinase 3	[more]
AT2G43790.1	1.6e-145	79.41	MAP kinase 6	[more]
AT4G01370.1	5.1e-128	69.51	MAP kinase 4	[more]
AT4G11330.1	5.9e-124	68.09	MAP kinase 5	[more]
AT1G07880.2	3.2e-122	67.33	Protein kinase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 1..261 e-value: 1.0E-73 score: 260.9
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 2..261 e-value: 7.7E-67 score: 225.4
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 1..261 score: 42.322155
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 60..288 e-value: 3.1E-146 score: 488.4
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 2..298 e-value: 3.1E-146 score: 488.4
None	No IPR available	PIRSR	PIRSR037014-2	PIRSR037014-2	coord: 2..167 e-value: 5.5E-22 score: 75.7
None	No IPR available	PIRSR	PIRSR000556-2	PIRSR000556-2	coord: 2..171 e-value: 1.2E-13 score: 48.4
None	No IPR available	PANTHER	PTHR24055	MITOGEN-ACTIVATED PROTEIN KINASE	coord: 1..306
None	No IPR available	PANTHER	PTHR24055:SF502	MITOGEN-ACTIVATED PROTEIN KINASE	coord: 1..306
IPR003527	Mitogen-activated protein (MAP) kinase, conserved site	PROSITE	PS01351	MAPK	coord: 11..114
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 98..110
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 1..268

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Chy12G207220	Chy12G207220	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Chy12G207220.1.exon1	Chy12G207220.1.exon1	exon
Chy12G207220.1.exon2	Chy12G207220.1.exon2	exon
Chy12G207220.1.exon3	Chy12G207220.1.exon3	exon
Chy12G207220.1.exon4	Chy12G207220.1.exon4	exon
Chy12G207220.1.exon5	Chy12G207220.1.exon5	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Chy12G207220.1.cds1	Chy12G207220.1.cds1	CDS
Chy12G207220.1.cds2	Chy12G207220.1.cds2	CDS
Chy12G207220.1.cds3	Chy12G207220.1.cds3	CDS
Chy12G207220.1.cds4	Chy12G207220.1.cds4	CDS
Chy12G207220.1.cds5	Chy12G207220.1.cds5	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Chy12G207220.1	Chy12G207220.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
biological_process	GO:1901002	positive regulation of response to salt stress
biological_process	GO:0009611	response to wounding
biological_process	GO:0009414	response to water deprivation
biological_process	GO:0010224	response to UV-B
biological_process	GO:0006979	response to oxidative stress
biological_process	GO:1902065	response to L-glutamate
biological_process	GO:0050826	response to freezing
biological_process	GO:0010200	response to chitin
biological_process	GO:0080136	priming of cellular response to stress
biological_process	GO:0010183	pollen tube guidance
biological_process	GO:0009875	pollen-pistil interaction
biological_process	GO:0009555	pollen development
biological_process	GO:0048481	plant ovule development
biological_process	GO:0000165	MAPK cascade
biological_process	GO:0010229	inflorescence development
biological_process	GO:0050832	defense response to fungus
biological_process	GO:0042742	defense response to bacterium
biological_process	GO:0010120	camalexin biosynthetic process
cellular_component	GO:0005938	cell cortex
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity
molecular_function	GO:0004707	MAP kinase activity
molecular_function	GO:0004712	protein serine/threonine/tyrosine kinase activity