CcUC10G186400.1 (mRNA) Watermelon (PI 537277) v1

Overview


Name	CcUC10G186400.1
Type	mRNA
Organism	Citrullus colocynthis (Watermelon (PI 537277) v1)
Description	bifunctional nuclease 2-like
Location	CicolChr10: 483821 .. 486948 (-)
Sequence length	1453
RNA-Seq Expression	CcUC10G186400.1
Synteny	CcUC10G186400.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTGAAAGTCATAATTTAAGCTTAAAAAAAATTATCTTTGAAAGAAAAGTTGGAATGTTATTAATAATGTTTTCAAAGTGGAGGGGGGTTTGTTTGTATTTTGCCTCATCTTCCTTTCTCTGTCCATTTCCTCCAAATCTTCCTCTCCCAAAATCGATTCAGCGGATTGAAACAAACTCTTTCTGTAACAGCGAAAAGGAAGCGCTTTCAGGCCAACCCCACTTCCTTTCCCCTTCAATCTCCATCTTCTTCTTCTTCTTCTTCTTCTTCTTCCATTTTTCTCTGTTTTTCCCTCATTACAATGCTGGGTTTAGCCCTTCACTTCTGTCTTCCCACCATTTTTCCCGGATCCGCCATAGCCATGGATCACTCCTCTGCTTCTCGCCCCTCCTCTTCCCTTCACTTCTCTCTTCTCCGATCGCGTCCCAATGCGGTTCGCTCTCGCCGTCCCAAGTCGATCGTCATTTCTTGCCACTCTTCTCGTGATAGATCCACCCACAGTGATGATGATTCCCATCAGGATTATTTCGAGGCTTCGTTTCTTGTTTCAGGTTCTTGGTTTTACTTTTTGGGTTCCTTTTTTAGGGCTTTGATGATTGTTATAATCGCTGATCAAATCGAATCGGGTTTGTGGGTGTTTCTGATTCTCGTATTTTTTTGGTTTTTTCTGACTATTAGAGACTATCTATCACTATCGAATGTGGAAGAAGAGGTTTCAAGAGGACGCGAGTTTCAACCAGAAAGAATCGCGTGTCAATTCTCATTCACTTGGACTGGGGTTTTTTAGACGTTTTCGAAGCCCCACTGTGTTTCTCAAAATTTCTTGCAATGGAGACTTTCTACTGCCAATTGTCGTAGGTATATTGGACGATAACTTTGTCGTGATGGGATTGTGTTTTCTTATGAATTTTGTTGAAAAGACGCTGTATATTTTGGACTTGGTCTGCAGGGGAATATGCTATTGAGAAACTTATTGATTGTCAGCTTGGGATTGAAAATGGGGTTGGTTTTCTTTGTATTTGTTATTCATATTTTTGGCTTCTCATACTTTGTTGTTGGTATTGATTGCCAAACAATCCTGTTACAGGAGGCCCCGGATCTCTTTCAGTTTATACAAGACCTAATTGTGAAAGTTGGTTATGAGGTATGGAACTACCTTCTAACTTAGCCAATAAATACATGTTCAACCTTTATTATTATTTCTGAGTTCAACAATGTGGGGGATCGAACTTACCAGTGCCCTATCCATTAGTTATGCTCAAGTTGGCCTAGATTTTATAATTGGTTCAGTATTCTCTTGGAGAGAGTTCGTGGCATGAATGACAAGGATGCTAGGATGGAGTTTTCAGCTTTTGATTATGCACTTGCTTGAAATATAAATTGTTGCTTCCTACTATTAGGCATGGGAGAACCAAGAAATATATACATGAACTTTTTAATTTTTTCTATACAAGAACCAAGAGGTGGAAAAGGTCTGCTAGTTTTGTTTGAAACTCCATGTACCTGATGAGACAAGGTTATGTTCAAGAATATTAGCCTATTTGTCTCTTATATTCCAAAGTTTTGTGTAATTCGAGACATAATTCTTCATCTTTTTTATTGTTAATAAATTATTTGCTGTAAGCTAGATTCTGGGTGGTCAGTGATTAGTGATGATTATAATTCGTTCCTTCCTTGATTGTTTCTTTAATGTAATTGAGTAGTAAATCTAGAAGCTTGCACGGCTTCTGAATAAATAATTTGTACTTCTAATGTACTTTTTTTTCTGCTAACTATGAAAAAAATTATGTTATTACGAGCATAGGCTACCACAGCGAGAATTACTGAAAGAGTGGTGAACACTTACTTTGCGCGGCTCTTCTTGAGAAAGGTTAGCTCTTACAAATTCAGTCACGATTAGCCAATACATTTTAAGAAGTTTTCAATACATGTCATTTTTTTTTCCGTCTCACAACTATTTTCATCTGCAGGAAGGGGAAAATGAGATGTTAAGTGTGGATGCACGCCCCTCAGATGCCCTAAATATTGCATATCGGTGTAAGGTAGATATTCTATCATTAACTGTCAAGATCATTGCAGAACTTATGCAAGTTGCTGGAATGACTTAGTTTCGTTTAAAATGAAGTACAGTAGTATATATGCTAATTTGGATGCTTTACTTTGCACCCTCTCACCCCCTTTCATGACAGATAATAATTTCTTACTTATACTGAAACTTTGCGGTCACGCCATTTTTCTGTTTAACGAATGTGCAGATACCAGTACTTGTTAGTAAGCAGATTGTCTATGAGGATGCGATAAGAGTTAGTTACGGGTTTGGAAGATTGCACGAGAGGAAGTCATGTTTTGATGTTTTACTTGACTGGTATAAGTGCAAGCACCGTTGGCTTATTTGATAGACATCTTATCTAAAAATGATAATTTCTTGTTCAGTGCTGCAGATGGCCCAGATTTCTTGTCCGAGGAACTCGATATGCTGAAGAATATGAAAATCGCTATTTATGAAGAACGGTACAAAGATGCAGGTATAAGTAGGGAGGGGCTAAGTGCTGACCAATTTATTTTTATTGCAACAATGTCTATCTAGACGTTTTGTTTCTGATCGATTTCATGCAAACGAGGGTATTTATTATTGATAATTGTTCCCTTCAGCCATGTGGAGGGATAAGTTAACAAAGCTTCGCAAGTCGGTACATGAGGCGTAACTTCGATATTCAAACTGCATTTATCTGCACTTGGACTTGTTCCTGTAAGTAACTCTCTGTAATTGGCTGCTGTACGGGGAGAGGTACCATACAACGAAAGGTGTTTGAATTTTTCTGATACTACACCCTAACAGGGCGACTCTTTTGTTGGGTAGGAATTGTTCGCTCGCAGGCATGTCTTTGAACATTCAATTCAGCATTGGATGGATTAACACTGAGTGGACTGTTACACATCATTGCAGAAAATTATCCATAGTGATGGTCGGTCTGTACAGTTACAATTAAGAGTATGTAAATATTACATTTGGGTAGTCAATGTGTTGTATCTCTAAGATATGAATGAGATGGCTGATATCTTTTTCAGCTGTAAGTTTGCAGAGAATGCTAACATGAAAAGGAAGCTTTTTGTATATTCTAACAAA

mRNA sequence

ATTTGAAAGTCATAATTTAAGCTTAAAAAAAATTATCTTTGAAAGAAAAGTTGGAATGTTATTAATAATGTTTTCAAAGTGGAGGGGGGTTTGTTTGTATTTTGCCTCATCTTCCTTTCTCTGTCCATTTCCTCCAAATCTTCCTCTCCCAAAATCGATTCAGCGGATTGAAACAAACTCTTTCTGTAACAGCGAAAAGGAAGCGCTTTCAGGCCAACCCCACTTCCTTTCCCCTTCAATCTCCATCTTCTTCTTCTTCTTCTTCTTCTTCTTCCATTTTTCTCTGTTTTTCCCTCATTACAATGCTGGGTTTAGCCCTTCACTTCTGTCTTCCCACCATTTTTCCCGGATCCGCCATAGCCATGGATCACTCCTCTGCTTCTCGCCCCTCCTCTTCCCTTCACTTCTCTCTTCTCCGATCGCGTCCCAATGCGGTTCGCTCTCGCCGTCCCAAGTCGATCGTCATTTCTTGCCACTCTTCTCGTGATAGATCCACCCACAGTGATGATGATTCCCATCAGGATTATTTCGAGGCTTCGTTTCTTGTTTCAGACGCTGTATATTTTGGACTTGGTCTGCAGGGGAATATGCTATTGAGAAACTTATTGATTGTCAGCTTGGGATTGAAAATGGGGTTGGTTTTCTTTGTATTTGTTATTCATATTTTTGGCTTCTCATACTTTGTTGTTGGTATTGATTGCCAAACAATCCTGTTACAGGAGGCCCCGGATCTCTTTCAGTTTATACAAGACCTAATTGTGAAAGTTGGTTATGAGGCTACCACAGCGAGAATTACTGAAAGAGTGGTGAACACTTACTTTGCGCGGCTCTTCTTGAGAAAGGAAGGGGAAAATGAGATGTTAAGTGTGGATGCACGCCCCTCAGATGCCCTAAATATTGCATATCGGTGTAAGATACCAGTACTTGTTAGTAAGCAGATTGTCTATGAGGATGCGATAAGAGTTAGTTACGGGTTTGGAAGATTGCACGAGAGGAAGTCATGTTTTGATGTTTTACTTGACTGTGCTGCAGATGGCCCAGATTTCTTGTCCGAGGAACTCGATATGCTGAAGAATATGAAAATCGCTATTTATGAAGAACGGTACAAAGATGCAGCCATGTGGAGGGATAAGTTAACAAAGCTTCGCAAGTCGGTACATGAGGCGGCGACTCTTTTGTTGGGTAGGAATTGTTCGCTCGCAGGCATGTCTTTGAACATTCAATTCAGCATTGGATGGATTAACACTGAGTGGACTGTTACACATCATTGCAGAAAATTATCCATAGTGATGGTCGGTCTGTACAGTTACAATTAAGAGTATGTAAATATTACATTTGGGTAGTCAATGTGTTGTATCTCTAAGATATGAATGAGATGGCTGATATCTTTTTCAGCTGTAAGTTTGCAGAGAATGCTAACATGAAAAGGAAGCTTTTTGTATATTCTAACAAA

Coding sequence (CDS)

ATGCTGGGTTTAGCCCTTCACTTCTGTCTTCCCACCATTTTTCCCGGATCCGCCATAGCCATGGATCACTCCTCTGCTTCTCGCCCCTCCTCTTCCCTTCACTTCTCTCTTCTCCGATCGCGTCCCAATGCGGTTCGCTCTCGCCGTCCCAAGTCGATCGTCATTTCTTGCCACTCTTCTCGTGATAGATCCACCCACAGTGATGATGATTCCCATCAGGATTATTTCGAGGCTTCGTTTCTTGTTTCAGACGCTGTATATTTTGGACTTGGTCTGCAGGGGAATATGCTATTGAGAAACTTATTGATTGTCAGCTTGGGATTGAAAATGGGGTTGGTTTTCTTTGTATTTGTTATTCATATTTTTGGCTTCTCATACTTTGTTGTTGGTATTGATTGCCAAACAATCCTGTTACAGGAGGCCCCGGATCTCTTTCAGTTTATACAAGACCTAATTGTGAAAGTTGGTTATGAGGCTACCACAGCGAGAATTACTGAAAGAGTGGTGAACACTTACTTTGCGCGGCTCTTCTTGAGAAAGGAAGGGGAAAATGAGATGTTAAGTGTGGATGCACGCCCCTCAGATGCCCTAAATATTGCATATCGGTGTAAGATACCAGTACTTGTTAGTAAGCAGATTGTCTATGAGGATGCGATAAGAGTTAGTTACGGGTTTGGAAGATTGCACGAGAGGAAGTCATGTTTTGATGTTTTACTTGACTGTGCTGCAGATGGCCCAGATTTCTTGTCCGAGGAACTCGATATGCTGAAGAATATGAAAATCGCTATTTATGAAGAACGGTACAAAGATGCAGCCATGTGGAGGGATAAGTTAACAAAGCTTCGCAAGTCGGTACATGAGGCGGCGACTCTTTTGTTGGGTAGGAATTGTTCGCTCGCAGGCATGTCTTTGAACATTCAATTCAGCATTGGATGGATTAACACTGAGTGGACTGTTACACATCATTGCAGAAAATTATCCATAGTGATGGTCGGTCTGTACAGTTACAATTAA

Protein sequence

MLGLALHFCLPTIFPGSAIAMDHSSASRPSSSLHFSLLRSRPNAVRSRRPKSIVISCHSSRDRSTHSDDDSHQDYFEASFLVSDAVYFGLGLQGNMLLRNLLIVSLGLKMGLVFFVFVIHIFGFSYFVVGIDCQTILLQEAPDLFQFIQDLIVKVGYEATTARITERVVNTYFARLFLRKEGENEMLSVDARPSDALNIAYRCKIPVLVSKQIVYEDAIRVSYGFGRLHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRDKLTKLRKSVHEAATLLLGRNCSLAGMSLNIQFSIGWINTEWTVTHHCRKLSIVMVGLYSYN

Homology

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BLAST of CcUC10G186400.1 vs. NCBI nr
Match: XP_011650273.1 (bifunctional nuclease 2 [Cucumis sativus] >KGN55629.1 hypothetical protein Csa_011518 [Cucumis sativus])

HSP 1 Score: 414.5 bits (1064), Expect = 9.0e-112
Identity = 238/312 (76.28%), Postives = 249/312 (79.81%), Query Frame = 0


Query: 1   MLGLALHFCLPTIFPGSAIAMDHSSASRPSSSLHFSLLRSRPNAVRSRRPKSIVISCHSS 60
           MLGLA HFCLPTIF GSAIAMDH SASRPS SLHFSL RSRPNAVRSRRPKSI+ISCHSS
Sbjct: 1   MLGLAPHFCLPTIFFGSAIAMDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSILISCHSS 60

Query: 61  RDRSTHSDDD--SHQDYFEASFLVSDAVY--------FGLGLQGNMLLRNLLIVSLGL-- 120
           RDRSTHSDDD  SHQDYFEASFLVS+ +Y        F      N         SLGL  
Sbjct: 61  RDRSTHSDDDDNSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQRESRANSNSLGLGF 120

Query: 121 ----KMGLVFFV------FVIHIFGFSYFVVG-IDCQT-ILLQEAPDLFQFIQDLIVKVG 180
               +   VF        F++ I    Y +   IDCQ  I   EAPD+FQFIQDLIVKVG
Sbjct: 121 FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKVG 180

Query: 181 YEATTARITERVVNTYFARLFLRKEGENEMLSVDARPSDALNIAYRCKIPVLVSKQIVYE 240
           YEA TARITERVVNTYFARLFLRKEGE+EMLSVDARPSDALNIAYRCKIPVLVSKQIV+E
Sbjct: 181 YEAITARITERVVNTYFARLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVFE 240

Query: 241 DAIRVSYGFGRLHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD 289
           DAIRVSYGFGR+HERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD
Sbjct: 241 DAIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD 300

[Close]

BLAST of CcUC10G186400.1 vs. NCBI nr
Match: XP_038905904.1 (bifunctional nuclease 2 [Benincasa hispida] >XP_038905905.1 bifunctional nuclease 2 [Benincasa hispida] >XP_038905906.1 bifunctional nuclease 2 [Benincasa hispida])

HSP 1 Score: 414.5 bits (1064), Expect = 9.0e-112
Identity = 233/317 (73.50%), Postives = 246/317 (77.60%), Query Frame = 0


Query: 1   MLGLALHFCLPTIFPGSAIAMDHSSASRPSSSLHFSLLRSRPNAVRSRRPKSIVISCHSS 60
           MLGLA HFC PTIFPGSAIAMDH SASRPSSSLHFS+LRSRP  VRSRRPKSI+ISCHSS
Sbjct: 1   MLGLAPHFCFPTIFPGSAIAMDHPSASRPSSSLHFSVLRSRPIPVRSRRPKSILISCHSS 60

Query: 61  RDRSTHS-DDDSHQDYFEASFLVSDAVY---------------------------FGLGL 120
           RDRSTHS DDDSHQDYFEASFLVS+ +Y                            GLG 
Sbjct: 61  RDRSTHSHDDDSHQDYFEASFLVSETIYHYRMWKKRFQEDATFNQRESSRVNSHSLGLG- 120

Query: 121 QGNMLLRNLLIVSLGLKMGLVFFVFVIHIFGFSYFVVGIDCQT-ILLQEAPDLFQFIQDL 180
                 R     ++ LK+       +  + G       IDCQ  I   EAPD+FQFIQDL
Sbjct: 121 ----FFRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDL 180

Query: 181 IVKVGYEATTARITERVVNTYFARLFLRKEGENEMLSVDARPSDALNIAYRCKIPVLVSK 240
           IVKVGYEATTARITERVVNTYFARLFLRKEGENEMLSVDARPSDALNIAYRCKIPVLVSK
Sbjct: 181 IVKVGYEATTARITERVVNTYFARLFLRKEGENEMLSVDARPSDALNIAYRCKIPVLVSK 240

Query: 241 QIVYEDAIRVSYGFGRLHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDA 289
           QIV+ED IRVSYGFGR+HERKSCFDVLLDCAADGPDFLSEELDMLKNMK+AIYEERYKDA
Sbjct: 241 QIVFEDGIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDA 300

[Close]

BLAST of CcUC10G186400.1 vs. NCBI nr
Match: XP_022965292.1 (bifunctional nuclease 2-like [Cucurbita maxima] >XP_022965293.1 bifunctional nuclease 2-like [Cucurbita maxima] >XP_022965294.1 bifunctional nuclease 2-like [Cucurbita maxima])

HSP 1 Score: 407.9 bits (1047), Expect = 8.4e-110
Identity = 226/310 (72.90%), Postives = 248/310 (80.00%), Query Frame = 0


Query: 1   MLGLALHFCLPTIFPGSAIAMDHSSASRPSSSLHFSLLRSRPNAVRSRRPKSIVISCHSS 60
           MLGL  HF L T+FPGSAIAMDH SASRPSSSLHFS+LRSRPNAVRSRRPKSI++SCHSS
Sbjct: 1   MLGLVPHFSLRTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAVRSRRPKSILVSCHSS 60

Query: 61  RDRSTHSDDDSHQDYFEASFLVSDAVY--------FGLGLQGNMLLRNLLIVSLGL---- 120
           RDRSTHSDDDSHQDYFEASFLVS+ +Y        F      N +   +   SLGL    
Sbjct: 61  RDRSTHSDDDSHQDYFEASFLVSETIYHYRMWKKRFQEDTNFNQIESRVNSPSLGLGFFR 120

Query: 121 --KMGLVFFV------FVIHIFGFSYFVVGIDCQTILLQ--EAPDLFQFIQDLIVKVGYE 180
             +   VF        F++ I    Y +  +    + ++  EAPDLFQFIQDL+VKVGYE
Sbjct: 121 RFQSPTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYE 180

Query: 181 ATTARITERVVNTYFARLFLRKEGENEMLSVDARPSDALNIAYRCKIPVLVSKQIVYEDA 240
            TTARITERVVNTYFARLFLRK+GENE LSVDARPSDALNIAYRCKIPVLVSKQIVYEDA
Sbjct: 181 VTTARITERVVNTYFARLFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDA 240

Query: 241 IRVSYGFGRLHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRDKL 289
           IRVS+GFGR+ +RKSCFDVLLDCAADGPDFLSEELDMLKNMK+AIYEERYKDAAMWRDKL
Sbjct: 241 IRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKL 300

[Close]

BLAST of CcUC10G186400.1 vs. NCBI nr
Match: XP_008448511.1 (PREDICTED: bifunctional nuclease 2 isoform X1 [Cucumis melo] >XP_008448512.1 PREDICTED: bifunctional nuclease 2 isoform X1 [Cucumis melo])

HSP 1 Score: 406.4 bits (1043), Expect = 2.5e-109
Identity = 235/312 (75.32%), Postives = 249/312 (79.81%), Query Frame = 0


Query: 1   MLGLALHFCLPTIFPGSAIAMDHSSASRPSSSLHFSLLRSRPNAVRSRRPKSIVISCHSS 60
           MLGLA HF LPTIF GSAIAMDH SASRPS SLHFSL RSRPNAVRSRRPKSI+ISCHSS
Sbjct: 1   MLGLAPHFRLPTIFFGSAIAMDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSILISCHSS 60

Query: 61  RDRSTHSDDD--SHQDYFEASFLVSDAVY--------FGLGLQGNMLLRNLLIVSLGL-- 120
           RDRST+SDDD  SHQDYFEASFLVS+ +Y        F   +  N     +   SLGL  
Sbjct: 61  RDRSTNSDDDDNSHQDYFEASFLVSETIYHYRMWKKRFQEDVNFNQRESRVNSPSLGLGF 120

Query: 121 ----KMGLVFFV------FVIHIFGFSYFVVG-IDCQT-ILLQEAPDLFQFIQDLIVKVG 180
               +   VF        F++ I    Y +   IDCQ  I   EAPD+FQFIQ LIVKVG
Sbjct: 121 FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQGLIVKVG 180

Query: 181 YEATTARITERVVNTYFARLFLRKEGENEMLSVDARPSDALNIAYRCKIPVLVSKQIVYE 240
           YEA TARITERVVNTYFARLFLRKEGE+EMLSVDARPSDALNIAYRCKIPVLVSKQIV+E
Sbjct: 181 YEAITARITERVVNTYFARLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVFE 240

Query: 241 DAIRVSYGFGRLHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD 289
           DAIRVSYGFGR+HERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD
Sbjct: 241 DAIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD 300

[Close]

BLAST of CcUC10G186400.1 vs. NCBI nr
Match: KAG7015839.1 (Bifunctional nuclease 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 405.2 bits (1040), Expect = 5.5e-109
Identity = 224/310 (72.26%), Postives = 248/310 (80.00%), Query Frame = 0


Query: 1   MLGLALHFCLPTIFPGSAIAMDHSSASRPSSSLHFSLLRSRPNAVRSRRPKSIVISCHSS 60
           MLGLA HF L T+FPGSAIAMDH SASRPSSSLHFS+LRSRPNAV SRRPKSI++SCHSS
Sbjct: 1   MLGLAPHFSLRTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAVSSRRPKSILVSCHSS 60

Query: 61  RDRSTHSDDDSHQDYFEASFLVSDAVY--------FGLGLQGNMLLRNLLIVSLGL---- 120
           RDRSTHSDDDSHQDYFEASFLVS+ +Y        F      N +   +   SLGL    
Sbjct: 61  RDRSTHSDDDSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQIESRVNSPSLGLGFFR 120

Query: 121 --KMGLVFFV------FVIHIFGFSYFVVGIDCQTILLQ--EAPDLFQFIQDLIVKVGYE 180
             +   VF        F++ I    Y +  +    + ++  EAPDLFQFIQDL+VKVGYE
Sbjct: 121 RFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYE 180

Query: 181 ATTARITERVVNTYFARLFLRKEGENEMLSVDARPSDALNIAYRCKIPVLVSKQIVYEDA 240
            TTARITERVVNTY+ARLFLRK+GENE LSVDARPSDALNIAYRCKIPVLVSKQIVYEDA
Sbjct: 181 VTTARITERVVNTYYARLFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDA 240

Query: 241 IRVSYGFGRLHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRDKL 289
           IRVS+GFGR+ +RKSCFDVLLDCAADGPDFLSEELDMLKNMK+AIYEERYKDAAMWRDKL
Sbjct: 241 IRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKL 300

[Close]

BLAST of CcUC10G186400.1 vs. ExPASy Swiss-Prot
Match: Q6YZM6 (Bifunctional nuclease 2 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD2 PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 5.1e-25
Identity = 67/168 (39.88%), Postives = 97/168 (57.74%), Query Frame = 0


Query: 128 VVGIDCQTILLQEA--------PDLFQFIQDLIVKVGYEATTARITERVVNTYFARLFLR 187
           ++ ++  ++LL  A        P ++Q ++++I K+GYE    RI +R+   Y A LFL 
Sbjct: 152 IIVLEMPSVLLMAAIRNVHIARPTIYQVVKEMIDKMGYEVKLVRINKRIQEAYCAELFLT 211

Query: 188 KEGEN-EMLSVDARPSDALNIAYRCKIPVLVSKQIVYEDAIRVSYGFGRLHERKSCFDVL 247
           K G++ E ++ D RPSDA+NIA RCK+P+ V + + Y D IR S    R+       D L
Sbjct: 212 KVGDHTESITFDLRPSDAINIAVRCKVPIQVHRSLAYSDGIR-SVEPARMAIAAGMSDGL 271

Query: 248 LDCAADGPD----FLSEELDMLKNMKIAIYEERYKDAAMWRDKLTKLR 283
           L    D PD      ++E  +++NM IA  EERYKDAA WRDKL  LR
Sbjct: 272 LFTELDRPDGQPCVEAQEFGLIRNMLIAAVEERYKDAATWRDKLMLLR 318

[Close]

BLAST of CcUC10G186400.1 vs. ExPASy Swiss-Prot
Match: Q9FWS6 (Bifunctional nuclease 1 OS=Arabidopsis thaliana OX=3702 GN=BBD1 PE=2 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 3.3e-24
Identity = 65/168 (38.69%), Postives = 99/168 (58.93%), Query Frame = 0


Query: 128 VVGIDCQTILLQEA--------PDLFQFIQDLIVKVGYEATTARITERVVNTYFARLFLR 187
           ++ ++  ++LL  A        P ++Q +++++ K+GYE    R+T+RV   YFA+LFL 
Sbjct: 150 IIVLEMPSVLLMAAMTNVQIARPTMYQVVKEMVDKMGYEVRLVRVTKRVHEAYFAQLFLS 209

Query: 188 KEGE-NEMLSVDARPSDALNIAYRCKIPVLVSKQIVYEDAIRVSYGFGRLHERKSCFDVL 247
           K G  +E +S D RPSDA+NIA RCKIP+ V+K + Y D +RV    G++       D L
Sbjct: 210 KVGNASECVSFDLRPSDAINIAVRCKIPIQVNKYLAYSDGMRVIES-GKISTPAPASDGL 269

Query: 248 LDCAADGPD----FLSEELDMLKNMKIAIYEERYKDAAMWRDKLTKLR 283
           L    D P+      ++E ++L  M  A+ EERY +AA WRDKL + R
Sbjct: 270 LFTEQDRPNGQACLDTKEFNILSKMMQAVDEERYDEAAEWRDKLGQFR 316

[Close]

BLAST of CcUC10G186400.1 vs. ExPASy Swiss-Prot
Match: Q93VH2 (Bifunctional nuclease 2 OS=Arabidopsis thaliana OX=3702 GN=BBD2 PE=2 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 9.6e-24
Identity = 100/301 (33.22%), Postives = 153/301 (50.83%), Query Frame = 0


Query: 14  FPGSAIAMDHSSASRPSSSLHFSLLRSRPNAVRSRRPKSI--VISCHSSRDRSTHSD-DD 73
           F G+    D S A+  +  LH             RR KSI  + S HS    ST  + ++
Sbjct: 39  FWGNQTKNDKSQAATVNLRLHL------------RRYKSIKCLFSSHSDGTGSTAENFNE 98

Query: 74  SHQDYFEASFLVSDAVYFGLGLQGNMLL----RNLLIV----------------SLGLKM 133
           + +DY ++S L  +AV    G  G M+     R L  V                ++ LKM
Sbjct: 99  NDEDYVKSSVL--EAVEVKSGPDGFMVKMKDGRQLRCVHNNPQGGNLPNYAPHSAIVLKM 158

Query: 134 ----GLVFFVFVIHIFGFSYFVVGIDCQTILLQEAPDLFQFIQDLIVKVGYEATTARITE 193
               GL+  + V+ +      V+ +   T +    P ++Q ++D++ K+GYE    R+T 
Sbjct: 159 EDGTGLLLPIIVLEMPS----VLLMAAMTNVQIARPTMYQVVKDMVDKMGYEVRLVRVTT 218

Query: 194 RVVNTYFARLFLRKEGE-NEMLSVDARPSDALNIAYRCKIPVLVSKQIVYEDAIRVSYGF 253
           RV   YFA L+L K G+ ++ +S D RPSDA+NIA RCK+P+ V+K + Y D +RV    
Sbjct: 219 RVHEAYFAELYLSKVGDKSDCVSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRV-IDS 278

Query: 254 GRLHERKSCFDVLLDCAADGPD----FLSEELDMLKNMKIAIYEERYKDAAMWRDKLTKL 283
           G+L ++    D LL    D P+    F ++E D+++NM  A+ EERY +AA WRDKL K 
Sbjct: 279 GKLSKQTPASDGLLFTELDRPNGQPCFDTKEFDLVRNMMQAVDEERYDEAAEWRDKLGKF 320

[Close]

BLAST of CcUC10G186400.1 vs. ExPASy Swiss-Prot
Match: B8A8D2 (Bifunctional nuclease 1 OS=Oryza sativa subsp. indica OX=39946 GN=BBD1 PE=3 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 8.1e-23
Identity = 58/149 (38.93%), Postives = 93/149 (62.42%), Query Frame = 0


Query: 142 PDLFQFIQDLIVKVGYEATTARITERVVNTYFARLFLRKEG-ENEMLSVDARPSDALNIA 201
           P ++  ++++  ++GY     RITE V + Y++RL+L K G E E +S+D +PSDA+NIA
Sbjct: 181 PTIYNVVKEMTERMGYAVRLVRITEMVHDAYYSRLYLAKIGNEEETISLDLKPSDAINIA 240

Query: 202 YRCKIPVLVSKQIVYEDAIRV-------SYGFGRLHERKSCFDVLLDCAADGPDFLSEEL 261
           +RCK+P+ V+++I Y + ++V       SY      ++  C    LD   D P F ++E 
Sbjct: 241 FRCKVPIQVNRRIAYNNGLKVVQPTPSESYVSS---DQFQC--TRLDRPDDQPCFEAQEF 300

Query: 262 DMLKNMKIAIYEERYKDAAMWRDKLTKLR 283
           D+++NM +A  EERYKDAA +RD+L   R
Sbjct: 301 DLVRNMLVAAVEERYKDAAQYRDQLFMFR 324

[Close]

BLAST of CcUC10G186400.1 vs. ExPASy Swiss-Prot
Match: Q5N8J3 (Bifunctional nuclease 1 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD1 PE=2 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 8.1e-23
Identity = 56/144 (38.89%), Postives = 90/144 (62.50%), Query Frame = 0


Query: 142 PDLFQFIQDLIVKVGYEATTARITERVVNTYFARLFLRKEG-ENEMLSVDARPSDALNIA 201
           P ++  ++++  ++GY     RITE V + Y++RL+L K G E E +S+D +PSDA+NIA
Sbjct: 181 PTIYNVVKEMTERMGYAVRLVRITEMVHDAYYSRLYLAKIGNEEETISLDLKPSDAINIA 240

Query: 202 YRCKIPVLVSKQIVYEDAIRVSYGF-GRLHERKSCFD-VLLDCAADGPDFLSEELDMLKN 261
           +RCK+P+ V+++I Y + ++V        +     F    LD   D P F ++E D+++N
Sbjct: 241 FRCKVPIQVNRRIAYNNGLKVVQPTPSESYVSSDQFQYTRLDRPDDQPCFEAQEFDLVRN 300

Query: 262 MKIAIYEERYKDAAMWRDKLTKLR 283
           M +A  EERYKDAA +RD+L   R
Sbjct: 301 MLVAAVEERYKDAAQYRDQLFMFR 324

[Close]

BLAST of CcUC10G186400.1 vs. ExPASy TrEMBL
Match: A0A0A0L3L8 (BFN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G002490 PE=3 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 4.4e-112
Identity = 238/312 (76.28%), Postives = 249/312 (79.81%), Query Frame = 0


Query: 1   MLGLALHFCLPTIFPGSAIAMDHSSASRPSSSLHFSLLRSRPNAVRSRRPKSIVISCHSS 60
           MLGLA HFCLPTIF GSAIAMDH SASRPS SLHFSL RSRPNAVRSRRPKSI+ISCHSS
Sbjct: 1   MLGLAPHFCLPTIFFGSAIAMDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSILISCHSS 60

Query: 61  RDRSTHSDDD--SHQDYFEASFLVSDAVY--------FGLGLQGNMLLRNLLIVSLGL-- 120
           RDRSTHSDDD  SHQDYFEASFLVS+ +Y        F      N         SLGL  
Sbjct: 61  RDRSTHSDDDDNSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQRESRANSNSLGLGF 120

Query: 121 ----KMGLVFFV------FVIHIFGFSYFVVG-IDCQT-ILLQEAPDLFQFIQDLIVKVG 180
               +   VF        F++ I    Y +   IDCQ  I   EAPD+FQFIQDLIVKVG
Sbjct: 121 FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKVG 180

Query: 181 YEATTARITERVVNTYFARLFLRKEGENEMLSVDARPSDALNIAYRCKIPVLVSKQIVYE 240
           YEA TARITERVVNTYFARLFLRKEGE+EMLSVDARPSDALNIAYRCKIPVLVSKQIV+E
Sbjct: 181 YEAITARITERVVNTYFARLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVFE 240

Query: 241 DAIRVSYGFGRLHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD 289
           DAIRVSYGFGR+HERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD
Sbjct: 241 DAIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD 300

[Close]

BLAST of CcUC10G186400.1 vs. ExPASy TrEMBL
Match: A0A6J1HJX9 (bifunctional nuclease 2-like OS=Cucurbita maxima OX=3661 GN=LOC111465202 PE=3 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 4.1e-110
Identity = 226/310 (72.90%), Postives = 248/310 (80.00%), Query Frame = 0


Query: 1   MLGLALHFCLPTIFPGSAIAMDHSSASRPSSSLHFSLLRSRPNAVRSRRPKSIVISCHSS 60
           MLGL  HF L T+FPGSAIAMDH SASRPSSSLHFS+LRSRPNAVRSRRPKSI++SCHSS
Sbjct: 1   MLGLVPHFSLRTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAVRSRRPKSILVSCHSS 60

Query: 61  RDRSTHSDDDSHQDYFEASFLVSDAVY--------FGLGLQGNMLLRNLLIVSLGL---- 120
           RDRSTHSDDDSHQDYFEASFLVS+ +Y        F      N +   +   SLGL    
Sbjct: 61  RDRSTHSDDDSHQDYFEASFLVSETIYHYRMWKKRFQEDTNFNQIESRVNSPSLGLGFFR 120

Query: 121 --KMGLVFFV------FVIHIFGFSYFVVGIDCQTILLQ--EAPDLFQFIQDLIVKVGYE 180
             +   VF        F++ I    Y +  +    + ++  EAPDLFQFIQDL+VKVGYE
Sbjct: 121 RFQSPTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYE 180

Query: 181 ATTARITERVVNTYFARLFLRKEGENEMLSVDARPSDALNIAYRCKIPVLVSKQIVYEDA 240
            TTARITERVVNTYFARLFLRK+GENE LSVDARPSDALNIAYRCKIPVLVSKQIVYEDA
Sbjct: 181 VTTARITERVVNTYFARLFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDA 240

Query: 241 IRVSYGFGRLHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRDKL 289
           IRVS+GFGR+ +RKSCFDVLLDCAADGPDFLSEELDMLKNMK+AIYEERYKDAAMWRDKL
Sbjct: 241 IRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKL 300

[Close]

BLAST of CcUC10G186400.1 vs. ExPASy TrEMBL
Match: A0A1S3BJU6 (bifunctional nuclease 2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490665 PE=3 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 1.2e-109
Identity = 235/312 (75.32%), Postives = 249/312 (79.81%), Query Frame = 0


Query: 1   MLGLALHFCLPTIFPGSAIAMDHSSASRPSSSLHFSLLRSRPNAVRSRRPKSIVISCHSS 60
           MLGLA HF LPTIF GSAIAMDH SASRPS SLHFSL RSRPNAVRSRRPKSI+ISCHSS
Sbjct: 1   MLGLAPHFRLPTIFFGSAIAMDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSILISCHSS 60

Query: 61  RDRSTHSDDD--SHQDYFEASFLVSDAVY--------FGLGLQGNMLLRNLLIVSLGL-- 120
           RDRST+SDDD  SHQDYFEASFLVS+ +Y        F   +  N     +   SLGL  
Sbjct: 61  RDRSTNSDDDDNSHQDYFEASFLVSETIYHYRMWKKRFQEDVNFNQRESRVNSPSLGLGF 120

Query: 121 ----KMGLVFFV------FVIHIFGFSYFVVG-IDCQT-ILLQEAPDLFQFIQDLIVKVG 180
               +   VF        F++ I    Y +   IDCQ  I   EAPD+FQFIQ LIVKVG
Sbjct: 121 FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQGLIVKVG 180

Query: 181 YEATTARITERVVNTYFARLFLRKEGENEMLSVDARPSDALNIAYRCKIPVLVSKQIVYE 240
           YEA TARITERVVNTYFARLFLRKEGE+EMLSVDARPSDALNIAYRCKIPVLVSKQIV+E
Sbjct: 181 YEAITARITERVVNTYFARLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVFE 240

Query: 241 DAIRVSYGFGRLHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD 289
           DAIRVSYGFGR+HERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD
Sbjct: 241 DAIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD 300

[Close]

BLAST of CcUC10G186400.1 vs. ExPASy TrEMBL
Match: A0A6J1E735 (bifunctional nuclease 2-like OS=Cucurbita moschata OX=3662 GN=LOC111431208 PE=3 SV=1)

HSP 1 Score: 404.1 bits (1037), Expect = 5.9e-109
Identity = 224/310 (72.26%), Postives = 247/310 (79.68%), Query Frame = 0


Query: 1   MLGLALHFCLPTIFPGSAIAMDHSSASRPSSSLHFSLLRSRPNAVRSRRPKSIVISCHSS 60
           MLGLA HF L T+FPGSAIAMDH SASRPSSSLHFS+LRSRPNAV SRRPKSI++SCHSS
Sbjct: 1   MLGLAPHFSLRTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAVSSRRPKSILVSCHSS 60

Query: 61  RDRSTHSDDDSHQDYFEASFLVSDAVY--------FGLGLQGNMLLRNLLIVSLGL---- 120
           RDRSTHSDDDSHQDYFEASFLVS+ +Y        F      N +   +    LGL    
Sbjct: 61  RDRSTHSDDDSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQIESRVNSPLLGLGFFR 120

Query: 121 --KMGLVFFV------FVIHIFGFSYFVVGIDCQTILLQ--EAPDLFQFIQDLIVKVGYE 180
             +   VF        F++ I    Y +  +    + ++  EAPDLFQFIQDL+VKVGYE
Sbjct: 121 RFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYE 180

Query: 181 ATTARITERVVNTYFARLFLRKEGENEMLSVDARPSDALNIAYRCKIPVLVSKQIVYEDA 240
            TTARITERVVNTYFARLFLRK+GENE LSVDARPSDALNIAYRCKIPVLVSKQIVYEDA
Sbjct: 181 VTTARITERVVNTYFARLFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDA 240

Query: 241 IRVSYGFGRLHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRDKL 289
           IRVS+GFGR+ +RKSCFDVLLDCAADGPDFLSEELDMLKNMK+AIYEERYKDAAMWRDKL
Sbjct: 241 IRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKL 300

[Close]

BLAST of CcUC10G186400.1 vs. ExPASy TrEMBL
Match: A0A5D3CJ41 (Bifunctional nuclease 2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G00420 PE=3 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 1.6e-98
Identity = 214/298 (71.81%), Postives = 230/298 (77.18%), Query Frame = 0


Query: 1   MLGLALHFCLPTIFPGSAIAMDHSSASRPSSSLHFSLLRSRPNAVRSRRPKSIVISCHSS 60
           MLGLA HF LPTIF GSAIAMDH SASRPS SLHFSL RSRPNAVRSRRPKSI+ISCHSS
Sbjct: 1   MLGLAPHFRLPTIFFGSAIAMDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSILISCHSS 60

Query: 61  RDRSTHSDDDSHQDY---------FEASFLVSDAVYFGLGLQGNMLLRNLLIVSLGLKMG 120
           RDRST+     ++ +         F       ++   GLG       R     ++ LK+ 
Sbjct: 61  RDRSTNKTIYHYRMWKKRFQEDVNFNQRESRVNSPSLGLG-----FFRRFRSPTVFLKIS 120

Query: 121 LVFFVFVIHIFGFSYFVVGIDCQT-ILLQEAPDLFQFIQDLIVKVGYEATTARITERVVN 180
                 +  + G       IDCQ  I   EAPD+FQFIQ LIVKVGYEA TARITERVVN
Sbjct: 121 CNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQGLIVKVGYEAITARITERVVN 180

Query: 181 TYFARLFLRKEGENEMLSVDARPSDALNIAYRCKIPVLVSKQIVYEDAIRVSYGFGRLHE 240
           TYFARLFLRKEGE+EMLSVDARPSDALNIAYRCKIPVLVSKQIV+EDAIRVSYGFGR+HE
Sbjct: 181 TYFARLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVFEDAIRVSYGFGRVHE 240

Query: 241 RKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRDKLTKLRKSVHEA 289
           RKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRDKLTKLRKSVHEA
Sbjct: 241 RKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRDKLTKLRKSVHEA 293

[Close]

BLAST of CcUC10G186400.1 vs. TAIR 10
Match: AT5G66050.2 (Wound-responsive family protein )

HSP 1 Score: 157.5 bits (397), Expect = 1.8e-38
Identity = 75/146 (51.37%), Postives = 104/146 (71.23%), Query Frame = 0


Query: 139 QEAPDLFQFIQDLIVKVGYEATTARITERVVNTYFARLFLRKEGENEMLSVDARPSDALN 198
           +E PD FQF+  ++ K+GYE    ++T R+VNTY+A L L K G+ E + +D+RPSDA+N
Sbjct: 86  EECPDQFQFVSAVVDKLGYEVKMVKLTGRIVNTYYASLCLGKPGDIEAICIDSRPSDAIN 145

Query: 199 IAYRCKIPVLVSKQIVYEDAIRVSYGFGRLHERKSCFDVLLDCAADGPDFLSEELDMLKN 258
           +A  C+ P+ V+K IV E+AI++ YG GR    K  F+V+LD A DGPD LSEEL +++N
Sbjct: 146 VARACQAPIYVNKAIVLEEAIKIGYG-GRPQSAKPVFNVILDSAPDGPDPLSEELKLVRN 205

Query: 259 MKIAIYEERYKDAAMWRDKLTKLRKS 285
           M +A  EERY DAAMWRD+L  L+ S
Sbjct: 206 MDLASKEERYIDAAMWRDRLKNLQNS 230

[Close]

BLAST of CcUC10G186400.1 vs. TAIR 10
Match: AT5G66050.1 (Wound-responsive family protein )

HSP 1 Score: 157.5 bits (397), Expect = 1.8e-38
Identity = 75/146 (51.37%), Postives = 104/146 (71.23%), Query Frame = 0


Query: 139 QEAPDLFQFIQDLIVKVGYEATTARITERVVNTYFARLFLRKEGENEMLSVDARPSDALN 198
           +E PD FQF+  ++ K+GYE    ++T R+VNTY+A L L K G+ E + +D+RPSDA+N
Sbjct: 180 EECPDQFQFVSAVVDKLGYEVKMVKLTGRIVNTYYASLCLGKPGDIEAICIDSRPSDAIN 239

Query: 199 IAYRCKIPVLVSKQIVYEDAIRVSYGFGRLHERKSCFDVLLDCAADGPDFLSEELDMLKN 258
           +A  C+ P+ V+K IV E+AI++ YG GR    K  F+V+LD A DGPD LSEEL +++N
Sbjct: 240 VARACQAPIYVNKAIVLEEAIKIGYG-GRPQSAKPVFNVILDSAPDGPDPLSEELKLVRN 299

Query: 259 MKIAIYEERYKDAAMWRDKLTKLRKS 285
           M +A  EERY DAAMWRD+L  L+ S
Sbjct: 300 MDLASKEERYIDAAMWRDRLKNLQNS 324

[Close]

BLAST of CcUC10G186400.1 vs. TAIR 10
Match: AT1G75380.1 (bifunctional nuclease in basal defense response 1 )

HSP 1 Score: 114.0 bits (284), Expect = 2.3e-25
Identity = 65/168 (38.69%), Postives = 99/168 (58.93%), Query Frame = 0


Query: 128 VVGIDCQTILLQEA--------PDLFQFIQDLIVKVGYEATTARITERVVNTYFARLFLR 187
           ++ ++  ++LL  A        P ++Q +++++ K+GYE    R+T+RV   YFA+LFL 
Sbjct: 150 IIVLEMPSVLLMAAMTNVQIARPTMYQVVKEMVDKMGYEVRLVRVTKRVHEAYFAQLFLS 209

Query: 188 KEGE-NEMLSVDARPSDALNIAYRCKIPVLVSKQIVYEDAIRVSYGFGRLHERKSCFDVL 247
           K G  +E +S D RPSDA+NIA RCKIP+ V+K + Y D +RV    G++       D L
Sbjct: 210 KVGNASECVSFDLRPSDAINIAVRCKIPIQVNKYLAYSDGMRVIES-GKISTPAPASDGL 269

Query: 248 LDCAADGPD----FLSEELDMLKNMKIAIYEERYKDAAMWRDKLTKLR 283
           L    D P+      ++E ++L  M  A+ EERY +AA WRDKL + R
Sbjct: 270 LFTEQDRPNGQACLDTKEFNILSKMMQAVDEERYDEAAEWRDKLGQFR 316

[Close]

BLAST of CcUC10G186400.1 vs. TAIR 10
Match: AT1G75380.2 (bifunctional nuclease in basal defense response 1 )

HSP 1 Score: 114.0 bits (284), Expect = 2.3e-25
Identity = 65/168 (38.69%), Postives = 99/168 (58.93%), Query Frame = 0


Query: 128 VVGIDCQTILLQEA--------PDLFQFIQDLIVKVGYEATTARITERVVNTYFARLFLR 187
           ++ ++  ++LL  A        P ++Q +++++ K+GYE    R+T+RV   YFA+LFL 
Sbjct: 150 IIVLEMPSVLLMAAMTNVQIARPTMYQVVKEMVDKMGYEVRLVRVTKRVHEAYFAQLFLS 209

Query: 188 KEGE-NEMLSVDARPSDALNIAYRCKIPVLVSKQIVYEDAIRVSYGFGRLHERKSCFDVL 247
           K G  +E +S D RPSDA+NIA RCKIP+ V+K + Y D +RV    G++       D L
Sbjct: 210 KVGNASECVSFDLRPSDAINIAVRCKIPIQVNKYLAYSDGMRVIES-GKISTPAPASDGL 269

Query: 248 LDCAADGPD----FLSEELDMLKNMKIAIYEERYKDAAMWRDKLTKLR 283
           L    D P+      ++E ++L  M  A+ EERY +AA WRDKL + R
Sbjct: 270 LFTEQDRPNGQACLDTKEFNILSKMMQAVDEERYDEAAEWRDKLGQFR 316

[Close]

BLAST of CcUC10G186400.1 vs. TAIR 10
Match: AT1G75380.3 (bifunctional nuclease in basal defense response 1 )

HSP 1 Score: 114.0 bits (284), Expect = 2.3e-25
Identity = 65/168 (38.69%), Postives = 99/168 (58.93%), Query Frame = 0


Query: 128 VVGIDCQTILLQEA--------PDLFQFIQDLIVKVGYEATTARITERVVNTYFARLFLR 187
           ++ ++  ++LL  A        P ++Q +++++ K+GYE    R+T+RV   YFA+LFL 
Sbjct: 150 IIVLEMPSVLLMAAMTNVQIARPTMYQVVKEMVDKMGYEVRLVRVTKRVHEAYFAQLFLS 209

Query: 188 KEGE-NEMLSVDARPSDALNIAYRCKIPVLVSKQIVYEDAIRVSYGFGRLHERKSCFDVL 247
           K G  +E +S D RPSDA+NIA RCKIP+ V+K + Y D +RV    G++       D L
Sbjct: 210 KVGNASECVSFDLRPSDAINIAVRCKIPIQVNKYLAYSDGMRVIES-GKISTPAPASDGL 269

Query: 248 LDCAADGPD----FLSEELDMLKNMKIAIYEERYKDAAMWRDKLTKLR 283
           L    D P+      ++E ++L  M  A+ EERY +AA WRDKL + R
Sbjct: 270 LFTEQDRPNGQACLDTKEFNILSKMMQAVDEERYDEAAEWRDKLGQFR 316

The following BLAST results are available for this feature:

BLAST of CcUC10G186400.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011650273.1	9.0e-112	76.28	bifunctional nuclease 2 [Cucumis sativus] >KGN55629.1 hypothetical protein Csa_0...	[more]
XP_038905904.1	9.0e-112	73.50	bifunctional nuclease 2 [Benincasa hispida] >XP_038905905.1 bifunctional nucleas...	[more]
XP_022965292.1	8.4e-110	72.90	bifunctional nuclease 2-like [Cucurbita maxima] >XP_022965293.1 bifunctional nuc...	[more]
XP_008448511.1	2.5e-109	75.32	PREDICTED: bifunctional nuclease 2 isoform X1 [Cucumis melo] >XP_008448512.1 PRE...	[more]
KAG7015839.1	5.5e-109	72.26	Bifunctional nuclease 2 [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of CcUC10G186400.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6YZM6	5.1e-25	39.88	Bifunctional nuclease 2 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD2 PE=2 SV...	[more]
Q9FWS6	3.3e-24	38.69	Bifunctional nuclease 1 OS=Arabidopsis thaliana OX=3702 GN=BBD1 PE=2 SV=1	[more]
Q93VH2	9.6e-24	33.22	Bifunctional nuclease 2 OS=Arabidopsis thaliana OX=3702 GN=BBD2 PE=2 SV=1	[more]
B8A8D2	8.1e-23	38.93	Bifunctional nuclease 1 OS=Oryza sativa subsp. indica OX=39946 GN=BBD1 PE=3 SV=1	[more]
Q5N8J3	8.1e-23	38.89	Bifunctional nuclease 1 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD1 PE=2 SV...	[more]

BLAST of CcUC10G186400.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L3L8	4.4e-112	76.28	BFN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G002490 PE=3 SV...	[more]
A0A6J1HJX9	4.1e-110	72.90	bifunctional nuclease 2-like OS=Cucurbita maxima OX=3661 GN=LOC111465202 PE=3 SV...	[more]
A0A1S3BJU6	1.2e-109	75.32	bifunctional nuclease 2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490665 PE=3 ...	[more]
A0A6J1E735	5.9e-109	72.26	bifunctional nuclease 2-like OS=Cucurbita moschata OX=3662 GN=LOC111431208 PE=3 ...	[more]
A0A5D3CJ41	1.6e-98	71.81	Bifunctional nuclease 2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]

BLAST of CcUC10G186400.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G66050.2	1.8e-38	51.37	Wound-responsive family protein	[more]
AT5G66050.1	1.8e-38	51.37	Wound-responsive family protein	[more]
AT1G75380.1	2.3e-25	38.69	bifunctional nuclease in basal defense response 1	[more]
AT1G75380.2	2.3e-25	38.69	bifunctional nuclease in basal defense response 1	[more]
AT1G75380.3	2.3e-25	38.69	bifunctional nuclease in basal defense response 1	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (PI 537277) v1
Date Performed: 2022-01-31

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036104	Bifunctional nuclease superfamily	GENE3D	3.10.690.10	Bifunctional nuclease domain	coord: 127..223 e-value: 7.7E-21 score: 76.1
IPR036104	Bifunctional nuclease superfamily	SUPERFAMILY	103256	Hypothetical protein TM0160	coord: 141..222
IPR003729	Bifunctional nuclease domain	PFAM	PF02577	DUF151	coord: 145..221 e-value: 6.8E-14 score: 51.6
IPR003729	Bifunctional nuclease domain	PROSITE	PS51658	BFN	coord: 75..221 score: 17.928368
None	No IPR available	PANTHER	PTHR15160	VON HIPPEL-LINDAU PROTEIN	coord: 141..282

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CcUC10G186400	CcUC10G186400	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CcUC10G186400.1-exon	CcUC10G186400.1-exon-CicolChr10:483821..484108	exon
CcUC10G186400.1-exon	CcUC10G186400.1-exon-CicolChr10:484247..484295	exon
CcUC10G186400.1-exon	CcUC10G186400.1-exon-CicolChr10:484423..484514	exon
CcUC10G186400.1-exon	CcUC10G186400.1-exon-CicolChr10:484583..484692	exon
CcUC10G186400.1-exon	CcUC10G186400.1-exon-CicolChr10:484906..484977	exon
CcUC10G186400.1-exon	CcUC10G186400.1-exon-CicolChr10:485078..485143	exon
CcUC10G186400.1-exon	CcUC10G186400.1-exon-CicolChr10:485804..486027	exon
CcUC10G186400.1-exon	CcUC10G186400.1-exon-CicolChr10:486397..486948	exon

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CcUC10G186400.1-three_prime_utr	CcUC10G186400.1-three_prime_utr-CicolChr10:483821..483957	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CcUC10G186400.1-cds	CcUC10G186400.1-cds-CicolChr10:483958..484108	CDS
CcUC10G186400.1-cds	CcUC10G186400.1-cds-CicolChr10:484247..484295	CDS
CcUC10G186400.1-cds	CcUC10G186400.1-cds-CicolChr10:484423..484514	CDS
CcUC10G186400.1-cds	CcUC10G186400.1-cds-CicolChr10:484583..484692	CDS
CcUC10G186400.1-cds	CcUC10G186400.1-cds-CicolChr10:484906..484977	CDS
CcUC10G186400.1-cds	CcUC10G186400.1-cds-CicolChr10:485078..485143	CDS
CcUC10G186400.1-cds	CcUC10G186400.1-cds-CicolChr10:485804..486027	CDS
CcUC10G186400.1-cds	CcUC10G186400.1-cds-CicolChr10:486397..486646	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CcUC10G186400.1-five_prime_utr	CcUC10G186400.1-five_prime_utr-CicolChr10:486647..486948	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CcUC10G186400.1	CcUC10G186400.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
cellular_component	GO:0005634	nucleus
cellular_component	GO:0030891	VCB complex
molecular_function	GO:0004518	nuclease activity