CcUC06G123190.1 (mRNA) Watermelon (PI 537277) v1

Overview
NameCcUC06G123190.1
TypemRNA
OrganismCitrullus colocynthis (Watermelon (PI 537277) v1)
Description4-coumarate--CoA ligase
LocationCicolChr06: 25564221 .. 25570766 (-)
Sequence length2243
RNA-Seq ExpressionCcUC06G123190.1
SyntenyCcUC06G123190.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTTTTTTTAATCTAGCATTACAGAAAAATTGTGTAGGAAGAATTCTCTCTATAATCTCTCATTCTCTCACCAAAACTAGAGAATCCCACTATTCTCTTGATTATTCCTGAGAATTGCAATGAAGACTTTCTTGGTGGTGTCGCAAGAAGCTCTCAGTATATTCTGTTCAAAGGAGGCGTTAGTGTTCGAGAGCCCGTGAGAAGTAGGAAAATTTACCAAGAATTCAAAGGTAAAGTATTTCCTACTTAATACCTCTTCAAAGCATGCTAGTTTTCAATTGTTTATCTATATGATTATTCTCTAAATTAAAAATTTTTCAGAGGCATAACGCATTCATTCACTTTCGCCGACTCAATTCCTTCAACTAAAATTTTTTTCCTTATATTTTTTTCTTGCTTGCATTGGTAATTAAGCCACGTTGGTCCGAGTGGCACATCGAGTTGATTTTATTGTTAATTTGTTGTCGAGAAATTGGTCCTTTTTTTCTTTTTTTTGGGAGACAGTTGAGAAATTGGTTCTAAATTTTTTAAGGAAAAAAATACAATTTTATATACATTAAAGTGCTATATTATATTAAATTTAATTTACGCATAAATTTAAGCTTTTGGATCATCAATAATTTAACATGTATCAAAGTAGAAGGTAAGAAATGTACTGTTTTTATTTTTTCTTTGAACAATTTACATGTAGTATAGGGATCAGTAGAGACACTCGAGTATCTCAATTAGGTTGCACACCCTTAGTGTCCTTATCACATCCTAAATCTAAAAGCGCTTCATTGACCAAAGACCAATACAAAAAGAGCTAGCTAGAGATGATCTCATACACAAAAGTTCAATAATACAAATAGAGATGGGTTAGAGGAAAGTTTTCCAATTAAGTGCGAGATACAAGCTTCACAATATCTATACAAGAGAGATTTAGTATTGTGTTCAAAACTACTTAAGTTTTCAAGTCAATTCGTAATTTAACAATTATAGGTAAAAACAAAAATATATTTATTATTTTTTTTTGTAGATCAAGAAGTCGCTGAATACAACGACGTTGAAAATATAGTGACATCCTCTCACGTCATAAGCGTAGAATGTTGCTATATACAAATAATTTGCCCCATTATACACTATGCCTTTTGTGATGAACTGTGAAGAATGACAATTTAATTCTAAAAGTGTTCTTCACCTTGAATTAAGTCTAATCAAATAATGAAGAAGTTGTTTTTATGTCTAATTTAAGACATTTTCTTATTCTTGTAGACGAAATTATTAATAATGATGGATCCAAACAGTGTCACTTCTAATTAAATGGACAAAAACATAATCAATAGACAAATTTATGATAATTTTAGGAAGTAAACCTTTACACTGTTAAGAGGATAAAGGGTTTGGAATTTGCAAATATTATGGAGGGATTGAAAATGGGCCATAGAAATACAAAAATTCCATGAAAAGAGTCATCTAAAGACAAAAGAATTGAGTAAGTCGTTTCTACATAGTGTCAGATTGAAACTGTATTCATTCAACCTTTCTACCAAAGACAAAATCTGAACTAATTATTCCAACCCTTGAATTGATGTACTCTGCCATTTCTTACGCATATCTCTATTTGCATTAATTGTGTATAATTAATTTAGGATTAATTTTTGCGTATAAATTTTCATATACACACATGGCACATGTATATGAAAACAAATAAGACTCTCAACTATTATTATTATTATTATTTTACAATACAAAAATTAAACAATCAACCATCCATCTTTATTTGAAGCCTTTTTTTTATCAACATATATAATTCTGTACCTTTGAGGACATTAAATAAAATTGAAATGATAAAGATCATGTACACAAACAATATATAATTTTTTAAAATGAAAGGTCATACCAATTACTACTAAATTAAACTCGCTTTAGTATAACTCATTTGTTATTAATAATAACAATACTTACTTCATCAACAAGTTGCTAAGTGTCAATTTTTTTTTAATAAAAAAATATTTTTTTCTTTGATATACTAAAATGAGTGTAGCTTGTTAATGTTTTAAGAGCCTAAATTAGAAAACATAAATTAGTAGATGAAAACAAGGGTTGAAATGGAAATTGTTGTAATTTGTGTTAATTCCAATGCATGGTTGATTTATTTATTTAGTTTATTTGTAACAACTCTATAGTTTCACCAACCCTACAACTAGTAGCTATCTGATTTCTGCTCTCGTTCCACTCCACTCACCCAAATTAAATCCAACTTCTAACCTTATTCATTCATTCCTTTAATTCCTTCCATTTCAACCTCCCCAACTCCACCAATGGCGGCCGACGACGATTCCTCACCGGAGTTCATATTCCGGTCAAAACTCCCCGACATCCCCATCCCCACCCACCTCCCCTTGCACTCATATTGCTTCGAAAAAATCTCCGAATTCAAATACCGTCCATGTCTAATCAACGCCGCCACGGGCAACATCTATACCTACGACGAAGTCCACATAACATCCCGCCGAGTGGCCGCCGGTCTTCACAAGTTAGGCATCGGAAAAGGCGACGTCATCATGCTCCTCCTCCAAAACACCCCACAGTTCGTTTTAGCCTTCCTCGGCGCCTCCTACCTGGGCGCCGCCACCACGCCGGCGAACCCATTCTTCACGCCGGCGGAAATTGCAAAACAAGCGACGTCATCCGGCGCCAAACTTATCATAACCCAAGCCGCGTTTGCAGAAAAAGTGAAAAAACTATCTCAAGAAAACAAGGCGATCATCAAAGTAATCTTCATCGACGACGACCCTCCGCCGGACGGAGGATATCATTTTTCGTCGTTGACTGATGACGTGGCAAAAGAGGAGGAAAGGGAAATGGGAGATGTGAAAATCAGCCCAGACGACGTCGTTGCATTGCCTTACTCGTCTGGGACCACCGGTCTTCCAAAAGGAGTTATGCTGACTCATAAAGCTCTAGTGACCAGCGTGGCACAACAGGTGGACGGTGAGAACCCACACCTATATATCCGAAGCGACGACGTCGTTCTGTGCGTGCTTCCTTTGTTTCACATATATTCCCTAAACTCAATCATGATGTGTTCGTTACGAGTAGGAGCTGCGATTTTAATCGTACAAAAATTCGACGTTAATTCGCTTCTGGAATTGATTCCTAAATATAGAGTCACCATTGCACCCATTGTTCCTCCTATTGTACTTGCAATTGCCAAGAGTCCGGCTCTGGACCATTACGACATGTCGTCCATACGTATTGTGATGTCGGGAGCCGCGCCCTTGGGGAAAACTCTTGAAGATGCCTTTAGAGCCAAGCTTCCTCATGTCATTCTCGGCCAGGTAAATTTACACTAATTTTATCGTCAATCAAAATTGTCAGTCACATTTGTAACAAGTATGAAATAATTTAAAGGGATACGGAATGACGGAGTCGGGGTCCGCGATGACAATGTCGTTGGCGTTTGCAAAGGAGAAGTTCGAGATGAAATCGGGAGGTTGTGGGACGTTAATGAGAAATTCAGAGATGAAGATTGTAAACCCTCAAACGGGAGCGTCTCTTCCAAGGAATCAAGCTGGGGAGATTTGTATTAGAAGTCCTCAGATTATGAAAGGTACACAATGCATTGTTTATTTGTCTATGTTCTAATTTAATGTGGTATGATTGGAATTTACTTCGGCGGGATAGATTATTTTATCTTTAGTACGAAACGTATATAGTTTTTTTTTAATTTAATTTTTGGCCATATATATGAGTTGGAGAAATCCTTTTTTTAGGTTGAGTTGATATTACAACATAGTTCTGAAAGGAGGGTATGAGTGGGATTTAGGAGAATTTGAACCAATGACCTTTTGATTATTGACATATACTATGTTGGTAGAGCTATATATGTTCGCTTTGCTCCTCTATAGTTCGTTGATAGTGTTGTAGTCAATCTCATTATGTTAGTATTAATATTAATATATCTAAAAAGTTAAAATCTCGATATCGATATTGACATCAATATTTTAATTATTGATTTTAAATGATCATCAATCTTGATGTTGAACCCGTTAATATTTTAAGAAAAATGTTGGTGATTGCACATGTCTTTGTGCAACCATTATTCAATAAAATTTTGCTATATGTCAATATATTTTTTTCTTTTTAAAAATTTGCTATATGTCAATATATTTTTTTCTTTTTAAAAATTGACTTGTGTTGTTTACTACTATATATATGTACAAAAGAAAGATGCATGTAATTAGGTGCTACTGATACTTGCCCATATTTTAATCTTATAATCTCAACGGAACTTGATATCAACTTTATCTTAAATTTTAAATACTTTTTAAATTAGAAAATTTTAGTTTTATTTTATAGTCTAGTTCGATTCTTTTGGTCAAATTTCTTTATGAATTTAAAAGTTTATTAGGCATGCCCAAAGCGAGCATGACATGTATTTATCTAAATGAAGTTTAGTTGAACTTTCAATTCACGTTTTTAAAAAATTTTATTATCTACTTTTTAATATACATTCATGTTTATTATCGTTGTAAATACATGTGAGTGACAAATATGTTATCTAACTAATAAGTCACATCACTCGTTACGTTTTCTTTTCCTTAAACATTGAAATTAAACCTGAAAATGTGAAAAAAAATCAAGGCAAATATCAATAATTGAATTAAAACTAGTTGCTTCAATTTACATCTAAATTAAATGTCTAAGATATGAATATCATTTCATAAATTAAAATAAAAACTGGTAAAAAAAATGGAAACTAAAACTAGTATTTGATTAAGCAATCTAGTTATTAGTTCAATTTAATAATTCTTCATATATATTAAAGTACAACATATAGGGTATAGAAATTTAAACTTCTAACTTTAAGAAAAAGAGTTCATTTCAATTGAAGTCGAGCGATATGTTCATTTGACCAATATTAACATTTTTTTAGTAGAACAATTACATAAATGGAGAATCAAACTTTTGACTACTTAAATTAAACCCACTTTAACAAGTAATAACATTAATATGTTACCATTTGGGCATTCACAATCCATCAACATAGTCAAAATAAATAATGTACCTATTACCTTATTTGAAGTGAAGTGTTGATGCCGCTAGTTTTACTATTGATGTACTAAAGAAGAAACAAAATTATCTTTTGACGGAAAGATTTAATTAATGAACCACATGTAATGGTGGAGTTGGCAGCTGATGTCACTAATTAATGCCACTAAATGCCGCCTAGTCATTCCAACAAAAAAATGGTACTTTGCCAATTAATACCGCCTAGCCAAATAGCGGAGTTAGCCAAGCAACAATTTGGGCCCAACCCATAGTTATTGGGCCTCAATTACCCAAGACCAAACCAAGCCCAATAGAATCGACAGAGTCCATTACATTGCTGTGTTCCGGTTTTGGTTGGTTGAATTGAACAGGATACCTCAACGACGAAGAGGCCACGAGGGGCATAATTGATAAAGATGGATGGCTGCACACCGGCGATATAGGTTTCGTCGATGACGACGACGAGGTCTTCATCGTCGATCGTCTCAAGGAGCTGATCAAATACAAAGGCTTCCAAGTGGCGCCGGCGGAGTTGGAAGCCCTGCTTATATCCCACGGTCGCATCGCTGATGTCGCGGTTATACCGTACGTCAGCCTTCTCTTTCCGATTCCTTCACTCGTGCTTTGTTCGATTTAACTGCTGCGTTTTATTTCACGCTAATTTGATAACATTTCAGTATGAAAGATGAAGTTGCTGGAGAGGTTCCGGTTGCGTTCATTGTCCGATCCGACGGTTCCAAAATCACAGAGGATGAAATTAAGCAATACATCTCCAAACAGGTAAAACCTCCGATTTTGGCATTAATTTCAAGCTTTCAGGTTTGATATCTCATTAGCTTTTGTATATTGATGGAGATTTTTGCATCGATTTCACAGTAAAAATTAGGTAACCTTCCTTTGACATAACTCATCGTAGGCCCCTGCTTTTTCGATTCCTTTCCTTATTTTCTATAATTTCTTAACCTTTCACCATGTCTATGCCTAACTTGAAAAGTTGTGATCAATACACTTTACATAATTCTAATAATTGGTTGGTTGAACATTTAATTTTGACATTGATTTTAACTTTTTAGGCACAATATTGAAGGTTTGAAGATCTATAGAAAACTTCTGAGATTCAAAAGCCTTATAAAAAACCAAATTAATAGTTCAATCTTAATTGAAATGTGGAAGATGATTGTAACTATTGAGGATTGTGGGATGCAGGTTGTGTTCTATAAGAGGATTAATCGTGTCTTCTTCGTGGATTCTATTCCTAAAAGTCAATCTGGCAAAATCTTACGGAGACAACTTAGAGCTTTACTCGCAGCTAGTATTCCTAATTAATTAGACAACAAACCTTACAACTCCCAATAATTCAACTCTCTCTCTCTCTCTTTCCTAGATATTTGTACTGTATGTAAAATAGAGAGAATGAACTTCTTTCTGGATTATGTTTATATGAAGTTCTGTTCAAGGGCTGCGTGGAAGTCGTTCTCTATCTAGCAAAAAAAAAAAAAAAGAACTAA

mRNA sequence

TTTTTTTTTTAATCTAGCATTACAGAAAAATTGTGTAGGAAGAATTCTCTCTATAATCTCTCATTCTCTCACCAAAACTAGAGAATCCCACTATTCTCTTGATTATTCCTGAGAATTGCAATGAAGACTTTCTTGGTGGTGTCGCAAGAAGCTCTCAGTATATTCTGTTCAAAGGAGGCGTTAGTGTTCGAGAGCCCGTGAGAAGTAGGAAAATTTACCAAGAATTCAAAGTTTCACCAACCCTACAACTAGTAGCTATCTGATTTCTGCTCTCGTTCCACTCCACTCACCCAAATTAAATCCAACTTCTAACCTTATTCATTCATTCCTTTAATTCCTTCCATTTCAACCTCCCCAACTCCACCAATGGCGGCCGACGACGATTCCTCACCGGAGTTCATATTCCGGTCAAAACTCCCCGACATCCCCATCCCCACCCACCTCCCCTTGCACTCATATTGCTTCGAAAAAATCTCCGAATTCAAATACCGTCCATGTCTAATCAACGCCGCCACGGGCAACATCTATACCTACGACGAAGTCCACATAACATCCCGCCGAGTGGCCGCCGGTCTTCACAAGTTAGGCATCGGAAAAGGCGACGTCATCATGCTCCTCCTCCAAAACACCCCACAGTTCGTTTTAGCCTTCCTCGGCGCCTCCTACCTGGGCGCCGCCACCACGCCGGCGAACCCATTCTTCACGCCGGCGGAAATTGCAAAACAAGCGACGTCATCCGGCGCCAAACTTATCATAACCCAAGCCGCGTTTGCAGAAAAAGTGAAAAAACTATCTCAAGAAAACAAGGCGATCATCAAAGTAATCTTCATCGACGACGACCCTCCGCCGGACGGAGGATATCATTTTTCGTCGTTGACTGATGACGTGGCAAAAGAGGAGGAAAGGGAAATGGGAGATGTGAAAATCAGCCCAGACGACGTCGTTGCATTGCCTTACTCGTCTGGGACCACCGGTCTTCCAAAAGGAGTTATGCTGACTCATAAAGCTCTAGTGACCAGCGTGGCACAACAGGTGGACGGTGAGAACCCACACCTATATATCCGAAGCGACGACGTCGTTCTGTGCGTGCTTCCTTTGTTTCACATATATTCCCTAAACTCAATCATGATGTGTTCGTTACGAGTAGGAGCTGCGATTTTAATCGTACAAAAATTCGACGTTAATTCGCTTCTGGAATTGATTCCTAAATATAGAGTCACCATTGCACCCATTGTTCCTCCTATTGTACTTGCAATTGCCAAGAGTCCGGCTCTGGACCATTACGACATGTCGTCCATACGTATTGTGATGTCGGGAGCCGCGCCCTTGGGGAAAACTCTTGAAGATGCCTTTAGAGCCAAGCTTCCTCATGTCATTCTCGGCCAGGGATACGGAATGACGGAGTCGGGGTCCGCGATGACAATGTCGTTGGCGTTTGCAAAGGAGAAGTTCGAGATGAAATCGGGAGGTTGTGGGACGTTAATGAGAAATTCAGAGATGAAGATTGTAAACCCTCAAACGGGAGCGTCTCTTCCAAGGAATCAAGCTGGGGAGATTTGTATTAGAAGTCCTCAGATTATGAAAGGATACCTCAACGACGAAGAGGCCACGAGGGGCATAATTGATAAAGATGGATGGCTGCACACCGGCGATATAGGTTTCGTCGATGACGACGACGAGGTCTTCATCGTCGATCGTCTCAAGGAGCTGATCAAATACAAAGGCTTCCAAGTGGCGCCGGCGGAGTTGGAAGCCCTGCTTATATCCCACGGTCGCATCGCTGATGTCGCGGTTATACCTATGAAAGATGAAGTTGCTGGAGAGGTTCCGGTTGCGTTCATTGTCCGATCCGACGGTTCCAAAATCACAGAGGATGAAATTAAGCAATACATCTCCAAACAGGTAAAACCTCCGATTTTGGCATTAATTTCAAGCTTTCAGGTTGTGTTCTATAAGAGGATTAATCGTGTCTTCTTCGTGGATTCTATTCCTAAAAGTCAATCTGGCAAAATCTTACGGAGACAACTTAGAGCTTTACTCGCAGCTAGTATTCCTAATTAATTAGACAACAAACCTTACAACTCCCAATAATTCAACTCTCTCTCTCTCTCTTTCCTAGATATTTGTACTGTATGTAAAATAGAGAGAATGAACTTCTTTCTGGATTATGTTTATATGAAGTTCTGTTCAAGGGCTGCGTGGAAGTCGTTCTCTATCTAGCAAAAAAAAAAAAAAAGAACTAA

Coding sequence (CDS)

ATGGCGGCCGACGACGATTCCTCACCGGAGTTCATATTCCGGTCAAAACTCCCCGACATCCCCATCCCCACCCACCTCCCCTTGCACTCATATTGCTTCGAAAAAATCTCCGAATTCAAATACCGTCCATGTCTAATCAACGCCGCCACGGGCAACATCTATACCTACGACGAAGTCCACATAACATCCCGCCGAGTGGCCGCCGGTCTTCACAAGTTAGGCATCGGAAAAGGCGACGTCATCATGCTCCTCCTCCAAAACACCCCACAGTTCGTTTTAGCCTTCCTCGGCGCCTCCTACCTGGGCGCCGCCACCACGCCGGCGAACCCATTCTTCACGCCGGCGGAAATTGCAAAACAAGCGACGTCATCCGGCGCCAAACTTATCATAACCCAAGCCGCGTTTGCAGAAAAAGTGAAAAAACTATCTCAAGAAAACAAGGCGATCATCAAAGTAATCTTCATCGACGACGACCCTCCGCCGGACGGAGGATATCATTTTTCGTCGTTGACTGATGACGTGGCAAAAGAGGAGGAAAGGGAAATGGGAGATGTGAAAATCAGCCCAGACGACGTCGTTGCATTGCCTTACTCGTCTGGGACCACCGGTCTTCCAAAAGGAGTTATGCTGACTCATAAAGCTCTAGTGACCAGCGTGGCACAACAGGTGGACGGTGAGAACCCACACCTATATATCCGAAGCGACGACGTCGTTCTGTGCGTGCTTCCTTTGTTTCACATATATTCCCTAAACTCAATCATGATGTGTTCGTTACGAGTAGGAGCTGCGATTTTAATCGTACAAAAATTCGACGTTAATTCGCTTCTGGAATTGATTCCTAAATATAGAGTCACCATTGCACCCATTGTTCCTCCTATTGTACTTGCAATTGCCAAGAGTCCGGCTCTGGACCATTACGACATGTCGTCCATACGTATTGTGATGTCGGGAGCCGCGCCCTTGGGGAAAACTCTTGAAGATGCCTTTAGAGCCAAGCTTCCTCATGTCATTCTCGGCCAGGGATACGGAATGACGGAGTCGGGGTCCGCGATGACAATGTCGTTGGCGTTTGCAAAGGAGAAGTTCGAGATGAAATCGGGAGGTTGTGGGACGTTAATGAGAAATTCAGAGATGAAGATTGTAAACCCTCAAACGGGAGCGTCTCTTCCAAGGAATCAAGCTGGGGAGATTTGTATTAGAAGTCCTCAGATTATGAAAGGATACCTCAACGACGAAGAGGCCACGAGGGGCATAATTGATAAAGATGGATGGCTGCACACCGGCGATATAGGTTTCGTCGATGACGACGACGAGGTCTTCATCGTCGATCGTCTCAAGGAGCTGATCAAATACAAAGGCTTCCAAGTGGCGCCGGCGGAGTTGGAAGCCCTGCTTATATCCCACGGTCGCATCGCTGATGTCGCGGTTATACCTATGAAAGATGAAGTTGCTGGAGAGGTTCCGGTTGCGTTCATTGTCCGATCCGACGGTTCCAAAATCACAGAGGATGAAATTAAGCAATACATCTCCAAACAGGTAAAACCTCCGATTTTGGCATTAATTTCAAGCTTTCAGGTTGTGTTCTATAAGAGGATTAATCGTGTCTTCTTCGTGGATTCTATTCCTAAAAGTCAATCTGGCAAAATCTTACGGAGACAACTTAGAGCTTTACTCGCAGCTAGTATTCCTAATTAA

Protein sequence

MAADDDSSPEFIFRSKLPDIPIPTHLPLHSYCFEKISEFKYRPCLINAATGNIYTYDEVHITSRRVAAGLHKLGIGKGDVIMLLLQNTPQFVLAFLGASYLGAATTPANPFFTPAEIAKQATSSGAKLIITQAAFAEKVKKLSQENKAIIKVIFIDDDPPPDGGYHFSSLTDDVAKEEEREMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKALVTSVAQQVDGENPHLYIRSDDVVLCVLPLFHIYSLNSIMMCSLRVGAAILIVQKFDVNSLLELIPKYRVTIAPIVPPIVLAIAKSPALDHYDMSSIRIVMSGAAPLGKTLEDAFRAKLPHVILGQGYGMTESGSAMTMSLAFAKEKFEMKSGGCGTLMRNSEMKIVNPQTGASLPRNQAGEICIRSPQIMKGYLNDEEATRGIIDKDGWLHTGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGRIADVAVIPMKDEVAGEVPVAFIVRSDGSKITEDEIKQYISKQVKPPILALISSFQVVFYKRINRVFFVDSIPKSQSGKILRRQLRALLAASIPN
Homology
BLAST of CcUC06G123190.1 vs. NCBI nr
Match: XP_038878634.1 (4-coumarate--CoA ligase 1-like [Benincasa hispida])

HSP 1 Score: 955.7 bits (2469), Expect = 1.8e-274
Identity = 495/568 (87.15%), Postives = 513/568 (90.32%), Query Frame = 0

Query: 1   MAADDDSSPEFIFRSKLPDIPIPTHLPLHSYCFEKISEFKYRPCLINAATGNIYTYDEVH 60
           MAA +DSSPEFIFRSKLP+I IPTHLPLH+Y FEK+SEF  RPCLINA TG  +TY EVH
Sbjct: 1   MAATEDSSPEFIFRSKLPEISIPTHLPLHTYLFEKVSEFTNRPCLINATTGETFTYGEVH 60

Query: 61  ITSRRVAAGLHKLGIGKGDVIMLLLQNTPQFVLAFLGASYLGAATTPANPFFTPAEIAKQ 120
            TSRRVAAGLHKLGI KGDVIMLLLQNTPQFV AFLGASYLGAA T ANPFFTPAEI KQ
Sbjct: 61  ETSRRVAAGLHKLGIRKGDVIMLLLQNTPQFVFAFLGASYLGAAATTANPFFTPAEITKQ 120

Query: 121 ATSSGAKLIITQAAFAEKVKKLSQENKAIIKVIFI----DDDPPPDGGYHFSSLTDDVAK 180
           A SS  KLIITQAAFAEKVKKLSQENKAIIKVIFI    DDDPPPDGGYHFSSLTDDVAK
Sbjct: 121 AASSSIKLIITQAAFAEKVKKLSQENKAIIKVIFIDDDDDDDPPPDGGYHFSSLTDDVAK 180

Query: 181 EEEREMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKALVTSVAQQVDGENPHLYIRSDD 240
           EEE EMGD K+SPDDVVALPYSSGTTGLPKGVMLTHK LV  VAQQVDGENPH  I+SDD
Sbjct: 181 EEEMEMGDAKVSPDDVVALPYSSGTTGLPKGVMLTHKGLVAIVAQQVDGENPHFNIQSDD 240

Query: 241 VVLCVLPLFHIYSLNSIMMCSLRVGAAILIVQKFDVNSLLELIPKYRVTIAPIVPPIVLA 300
           VVLCVLPLFHIYSLNSIMMCSLRVGAAILIVQKFDVN LL LIPKY+VTIAPIVPPIVLA
Sbjct: 241 VVLCVLPLFHIYSLNSIMMCSLRVGAAILIVQKFDVNLLLGLIPKYKVTIAPIVPPIVLA 300

Query: 301 IAKSPALDHYDMSSIRIVMSGAAPLGKTLEDAFRAKLPHVILGQGYGMTESGSAMTMSLA 360
           I KSPA+DH DMSS+RIVMSGAAPLGK LEDAFR KLPHVILGQGYGMTESGSAMTMSLA
Sbjct: 301 IVKSPAVDHSDMSSLRIVMSGAAPLGKNLEDAFRTKLPHVILGQGYGMTESGSAMTMSLA 360

Query: 361 FAKEKFEMKSGGCGTLMRNSEMKIVNPQTGASLPRNQAGEICIRSPQIMKGYLNDEEATR 420
           FAKE FE+KSGGCGT+MRN+EMKI+NPQTGASLPRNQAGEICI+S QIMKGYLNDEEAT+
Sbjct: 361 FAKEGFEIKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIKSLQIMKGYLNDEEATK 420

Query: 421 GIIDKDGWLHTGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGRIADVAV 480
           GIIDKDGWLHTGD+GFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHG IAD AV
Sbjct: 421 GIIDKDGWLHTGDVGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAV 480

Query: 481 IPMKDEVAGEVPVAFIVRSDGSKITEDEIKQYISKQVKPPILALISSFQVVFYKRINRVF 540
           IPMKDEVAGEVPVAFI RSDG+ ITEDEIKQYISK             QVVFYKRINRVF
Sbjct: 481 IPMKDEVAGEVPVAFIARSDGTNITEDEIKQYISK-------------QVVFYKRINRVF 540

Query: 541 FVDSIPKSQSGKILRRQLRALLAASIPN 565
           FVDSIPKS SGKILRRQLRALLAASIPN
Sbjct: 541 FVDSIPKSPSGKILRRQLRALLAASIPN 555

BLAST of CcUC06G123190.1 vs. NCBI nr
Match: XP_008453615.1 (PREDICTED: 4-coumarate--CoA ligase 1-like [Cucumis melo] >TYK11846.1 4-coumarate--CoA ligase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 931.8 bits (2407), Expect = 2.8e-267
Identity = 482/563 (85.61%), Postives = 509/563 (90.41%), Query Frame = 0

Query: 1   MAADDDSSPEFIFRSKLPDIPIPTHLPLHSYCFEKISEFKYRPCLINAATGNIYTYDEVH 60
           MAA D SSPEF+FRSKLP+IPI THLPLH+YCFE ISEFK RPCLINAATG+ YTY EV 
Sbjct: 1   MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ 60

Query: 61  ITSRRVAAGLHKLGIGKGDVIMLLLQNTPQFVLAFLGASYLGAATTPANPFFTPAEIAKQ 120
            TSRRVAAGLHKLGIGKGDVIMLLLQNTP+FVLAFL ASYLGAA T ANPFF PAEIAKQ
Sbjct: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ 120

Query: 121 ATSSGAKLIITQAAFAEKVKKLSQENKAIIKVIFI-DDDPPPDGGYHFSSLTDDVAKEEE 180
           A SSGAK+IITQ+AFAEKVKKLSQ ++ IIKVIFI DDDPP  GGY FSSL DDVAKEEE
Sbjct: 121 AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEE 180

Query: 181 REMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKALVTSVAQQVDGENPHLYIRSDDVVL 240
            EMGDVKISPDDVVALPYSSGTTGLPKGVMLTHK  VTSVAQQVDGENPHLYIRSDDVVL
Sbjct: 181 LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL 240

Query: 241 CVLPLFHIYSLNSIMMCSLRVGAAILIVQKFDVNSLLELIPKYRVTIAPIVPPIVLAIAK 300
           C+LPLFHIYSLNSIMMCSLRVGAAILIVQKFD+NSLLEL+PKY+VT AP+VPPIVLAIAK
Sbjct: 241 CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK 300

Query: 301 SPALDHYDMSSIRIVMSGAAPLGKTLEDAFRAKLPHVILGQGYGMTESGSAMTMSLAFAK 360
           SPA+DH+DMSS+R+V+SGAAPLGK LEDAFRAKLPHVILGQGYGMTE+GS MTMSLAFAK
Sbjct: 301 SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK 360

Query: 361 EKFEMKSGGCGTLMRNSEMKIVNPQTGASLPRNQAGEICIRSPQIMKGYLNDEEATRGII 420
           E F +KSGGCGT+MRNSEMKIV PQTG SLPRN+ GEICIRS Q+MKGYLNDEEAT+ II
Sbjct: 361 EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII 420

Query: 421 DKDGWLHTGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGRIADVAVIPM 480
           DKDGWLHTGDIG+VDDDDE+FIVDRLKELIKYKGFQVAPAELEALLISHG IAD AVIPM
Sbjct: 421 DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 480

Query: 481 KDEVAGEVPVAFIVRSDGSKITEDEIKQYISKQVKPPILALISSFQVVFYKRINRVFFVD 540
            DEVAGEVPV FIVR DGS ITEDEIKQYISK             QVVFYKRINRVFFVD
Sbjct: 481 NDEVAGEVPVTFIVRFDGSNITEDEIKQYISK-------------QVVFYKRINRVFFVD 540

Query: 541 SIPKSQSGKILRRQLRALLAASI 563
           SIPKS SGKILRRQLRALLAASI
Sbjct: 541 SIPKSPSGKILRRQLRALLAASI 550

BLAST of CcUC06G123190.1 vs. NCBI nr
Match: KAA0058290.1 (4-coumarate--CoA ligase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 931.0 bits (2405), Expect = 4.8e-267
Identity = 482/565 (85.31%), Postives = 509/565 (90.09%), Query Frame = 0

Query: 1   MAADDDSSPEFIFRSKLPDIPIPTHLPLHSYCFEKISEFKYRPCLINAATGNIYTYDEVH 60
           MAA D SSPEF+FRSKLP+IPI THLPLH+YCFE ISEFK RPCLINAATG+ YTY EV 
Sbjct: 1   MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ 60

Query: 61  ITSRRVAAGLHKLGIGKGDVIMLLLQNTPQFVLAFLGASYLGAATTPANPFFTPAEIAKQ 120
             SRRVAAGLHKLGIGKGDVIMLLLQNTP+FVLAFL ASYLGAA T ANPFF PAEIAKQ
Sbjct: 61  AISRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ 120

Query: 121 ATSSGAKLIITQAAFAEKVKKLSQENKAIIKVIFI-DDDPPPDGGYHFSSLTDDVAKEEE 180
           A SSGAK+IITQ+AFAEKVKKLSQ ++ IIKVIFI DDDPP  GGY FSSL DDVAKEEE
Sbjct: 121 AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEE 180

Query: 181 REMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKALVTSVAQQVDGENPHLYIRSDDVVL 240
            EMGDVKISPDDVVALPYSSGTTGLPKGVMLTHK  VTSVAQQVDGENPHLYIRSDDVVL
Sbjct: 181 LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL 240

Query: 241 CVLPLFHIYSLNSIMMCSLRVGAAILIVQKFDVNSLLELIPKYRVTIAPIVPPIVLAIAK 300
           C+LPLFHIYSLNSIMMCSLRVGAAILIVQKFD+NSLLEL+PKY+VT AP+VPPIVLAIAK
Sbjct: 241 CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK 300

Query: 301 SPALDHYDMSSIRIVMSGAAPLGKTLEDAFRAKLPHVILGQGYGMTESGSAMTMSLAFAK 360
           SPA+DH+DMSS+R+V+SGAAPLGK LEDAFRAKLPHVILGQGYGMTE+GS MTMSLAFAK
Sbjct: 301 SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK 360

Query: 361 EKFEMKSGGCGTLMRNSEMKIVNPQTGASLPRNQAGEICIRSPQIMKGYLNDEEATRGII 420
           E F +KSGGCGT+MRNSEMKIV PQTG SLPRN+ GEICIRS Q+MKGYLNDEEAT+ II
Sbjct: 361 EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII 420

Query: 421 DKDGWLHTGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGRIADVAVIPM 480
           DKDGWLHTGDIG+VDDDDE+FIVDRLKELIKYKGFQVAPAELEALLISHG IAD AVIPM
Sbjct: 421 DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 480

Query: 481 KDEVAGEVPVAFIVRSDGSKITEDEIKQYISKQVKPPILALISSFQVVFYKRINRVFFVD 540
            DEVAGEVPV FIVR DGS ITEDEIKQYISK             QVVFYKRINRVFFVD
Sbjct: 481 NDEVAGEVPVTFIVRFDGSNITEDEIKQYISK-------------QVVFYKRINRVFFVD 540

Query: 541 SIPKSQSGKILRRQLRALLAASIPN 565
           SIPKS SGKILRRQLRALLAASI N
Sbjct: 541 SIPKSPSGKILRRQLRALLAASILN 552

BLAST of CcUC06G123190.1 vs. NCBI nr
Match: XP_004146311.1 (4-coumarate--CoA ligase 1 [Cucumis sativus] >KGN65444.1 hypothetical protein Csa_019996 [Cucumis sativus])

HSP 1 Score: 914.8 bits (2363), Expect = 3.5e-262
Identity = 474/562 (84.34%), Postives = 506/562 (90.04%), Query Frame = 0

Query: 1   MAADDDSSPEFIFRSKLPDIPIPTHLPLHSYCFEKISEFKYRPCLINAATGNIYTYDEVH 60
           MAA D SSPEFIFRSKLP+IPI THLPLH+YCFE ISEFK+RPCLINAATG+IYTY EV 
Sbjct: 1   MAAADASSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKHRPCLINAATGHIYTYGEVQ 60

Query: 61  ITSRRVAAGLHKLGIGKGDVIMLLLQNTPQFVLAFLGASYLGAATTPANPFFTPAEIAKQ 120
            TSRRVAAGLHKLGIGKGDVIMLLLQNTP+FVLAFLGASYLGAA T ANPFF  +EIAKQ
Sbjct: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLGASYLGAAATMANPFFMQSEIAKQ 120

Query: 121 ATSSGAKLIITQAAFAEKVKKLSQENKAIIKVIFIDDDPPPDGGYHFSSLTDDVAKEEER 180
           A SSGAK+IITQAAFAEKVK LSQEN+ IIKVIFIDD+ PP     FSSL +DVAKEEE 
Sbjct: 121 AVSSGAKVIITQAAFAEKVKNLSQENEMIIKVIFIDDNDPP----QFSSLIEDVAKEEEL 180

Query: 181 EMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKALVTSVAQQVDGENPHLYIRSDDVVLC 240
           EMGDVKISP+DVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPHL+IRSDDVVLC
Sbjct: 181 EMGDVKISPEDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLC 240

Query: 241 VLPLFHIYSLNSIMMCSLRVGAAILIVQKFDVNSLLELIPKYRVTIAPIVPPIVLAIAKS 300
           +LPLFHIYSLNSIMMC+LRVGAAIL+VQKFDVNSL+EL+ KY+VT AP VPPIVLAIAKS
Sbjct: 241 LLPLFHIYSLNSIMMCALRVGAAILLVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKS 300

Query: 301 PALDHYDMSSIRIVMSGAAPLGKTLEDAFRAKLPHVILGQGYGMTESGSAMTMSLAFAKE 360
           PA+DH+DMSS+RIV+SGAAPLGK LEDAFRAKLPHVILGQGYGMTE+GS MTMSLAF KE
Sbjct: 301 PAVDHFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKE 360

Query: 361 KFEMKSGGCGTLMRNSEMKIVNPQTGASLPRNQAGEICIRSPQIMKGYLNDEEATRGIID 420
            F +KSGGCGT+MRNSEMKI+N QTG SLPRNQ GEICIRS Q+MKGYLNDE+AT+ IID
Sbjct: 361 GFGIKSGGCGTIMRNSEMKILNLQTGESLPRNQTGEICIRSSQMMKGYLNDEDATKAIID 420

Query: 421 KDGWLHTGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGRIADVAVIPMK 480
           +DGWLHTGDIGFVDDDDE+FIVDRLKELIKYKGFQVAPAELEALLISH  IAD AVIPM 
Sbjct: 421 EDGWLHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMN 480

Query: 481 DEVAGEVPVAFIVRSDGSKITEDEIKQYISKQVKPPILALISSFQVVFYKRINRVFFVDS 540
           DEVAGEVPVAFIVR DGS ITEDEIKQ+ISK             QVVFYKRINRVFFVDS
Sbjct: 481 DEVAGEVPVAFIVRFDGSNITEDEIKQFISK-------------QVVFYKRINRVFFVDS 540

Query: 541 IPKSQSGKILRRQLRALLAASI 563
           IPKS SGKILRRQLRALLAASI
Sbjct: 541 IPKSPSGKILRRQLRALLAASI 545

BLAST of CcUC06G123190.1 vs. NCBI nr
Match: KAG6589713.1 (4-coumarate--CoA ligase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 885.9 bits (2288), Expect = 1.8e-253
Identity = 460/559 (82.29%), Postives = 496/559 (88.73%), Query Frame = 0

Query: 6   DSSPEFIFRSKLPDIPIPTHLPLHSYCFEKISEFKYRPCLINAATGNIYTYDEVHITSRR 65
           DSSPEFIFRSKLPD+ IP HLPLH+YCFE+IS+FK+ PCLINAATG+IYTY EVH+T+RR
Sbjct: 5   DSSPEFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHVTARR 64

Query: 66  VAAGLHKLGIGKGDVIMLLLQNTPQFVLAFLGASYLGAATTPANPFFTPAEIAKQATSSG 125
           VAAG HKLGIGKGDVIMLLLQNTPQFV AFLGAS+  A  T ANPFFTPAEIAKQA SSG
Sbjct: 65  VAAGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQAASSG 124

Query: 126 AKLIITQAAFAEKVKKLSQENKAIIKVIFIDDDPPPDGGYHFSSLTDDVAKEEEREMGDV 185
           AKLIITQAAFAEKVKKL QEN+ I+KVIFID   PP+GG HF+SLTD+ AKEE  EM DV
Sbjct: 125 AKLIITQAAFAEKVKKLPQENQGILKVIFIDS--PPEGGCHFTSLTDE-AKEE--EMADV 184

Query: 186 KISPDDVVALPYSSGTTGLPKGVMLTHKALVTSVAQQVDGENPHLYIRSDDVVLCVLPLF 245
           K SPDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPH +IRS+DVVLCVLPLF
Sbjct: 185 KFSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLF 244

Query: 246 HIYSLNSIMMCSLRVGAAILIVQKFDVNSLLELIPKYRVTIAPIVPPIVLAIAKSPALDH 305
           HIYSL+SI+M SLRVGAAILIVQKFD+NSL+ L+PKYRVTIA +VPPIVLAI KSP +D 
Sbjct: 245 HIYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAIVKSPVVDQ 304

Query: 306 YDMSSIRIVMSGAAPLGKTLEDAFRAKLPHVILGQGYGMTESGSAMTMSLAFAKEKFEMK 365
            DMSSIRIV+SGAAPLGK LEDAFRAKLPH ILGQGYGMTESGS +TMSLA A+EKF +K
Sbjct: 305 SDMSSIRIVLSGAAPLGKNLEDAFRAKLPHAILGQGYGMTESGSVLTMSLALAREKFAIK 364

Query: 366 SGGCGTLMRNSEMKIVNPQTGASLPRNQAGEICIRSPQIMKGYLNDEEATRGIIDKDGWL 425
           SG CGTLMRN+EMKIVNP+TG SL RNQ GEI +RSPQ+MKGYLNDEEAT+GIID+DGWL
Sbjct: 365 SGACGTLMRNAEMKIVNPETGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGIIDEDGWL 424

Query: 426 HTGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGRIADVAVIPMKDEVAG 485
           HTGD+G VDDDDEVFIVDRLKELIKYKGFQVAPAELE LLIS+  I DVAVIPMKDEVAG
Sbjct: 425 HTGDMGLVDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAG 484

Query: 486 EVPVAFIVRSDGSKITEDEIKQYISKQVKPPILALISSFQVVFYKRINRVFFVDSIPKSQ 545
           EVPVAFIVRSDGS ITEDEIKQYISK             QVVFYKRINRVFFVDSIPKS 
Sbjct: 485 EVPVAFIVRSDGSNITEDEIKQYISK-------------QVVFYKRINRVFFVDSIPKSP 544

Query: 546 SGKILRRQLRALLAASIPN 565
           SGKILRRQLRALL A+IPN
Sbjct: 545 SGKILRRQLRALLPANIPN 545

BLAST of CcUC06G123190.1 vs. ExPASy Swiss-Prot
Match: M4ISH0 (4-coumarate--CoA ligase CCL1 OS=Humulus lupulus OX=3486 GN=CCL1 PE=1 SV=1)

HSP 1 Score: 755.4 bits (1949), Expect = 4.7e-217
Identity = 384/554 (69.31%), Postives = 452/554 (81.59%), Query Frame = 0

Query: 5   DDSSPEFIFRSKLPDIPIPTHLPLHSYCFEKISEFKYRPCLINAATGNIYTYDEVHITSR 64
           DD   EFIFRSKLPDI IP HLPLHSYCFE IS+FK RPCLIN ATG I TY +V +TSR
Sbjct: 7   DDHQEEFIFRSKLPDIYIPNHLPLHSYCFENISQFKDRPCLINGATGEIITYADVDLTSR 66

Query: 65  RVAAGLHKLGIGKGDVIMLLLQNTPQFVLAFLGASYLGAATTPANPFFTPAEIAKQATSS 124
           +VAAGL KLGI +GDVIMLLLQN+P+FV AFL ASY+GA  T ANPF+TPAE+AKQA +S
Sbjct: 67  KVAAGLDKLGIKQGDVIMLLLQNSPEFVYAFLAASYIGAIITTANPFYTPAEVAKQAAAS 126

Query: 125 GAKLIITQAAFAEKVKKLSQENKAIIKVIFIDDDPPPDGGYHFSSLTDDVAKEEEREMGD 184
             KL+IT A + +KVK+ +   ++ +KV+ +D  PP     HFS LT    + +E E+  
Sbjct: 127 KTKLVITLAGYIDKVKEFT-GGESGVKVMCVDAPPPESECLHFSELT----QADETEIPA 186

Query: 185 VKISPDDVVALPYSSGTTGLPKGVMLTHKALVTSVAQQVDGENPHLYIRSDDVVLCVLPL 244
           VKI PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDG+NP+LY   +DV+LCVLPL
Sbjct: 187 VKIHPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFHQNDVILCVLPL 246

Query: 245 FHIYSLNSIMMCSLRVGAAILIVQKFDVNSLLELIPKYRVTIAPIVPPIVLAIAKSPALD 304
           FHIYSLNSI++C LRVGAAILI+QKF+++ LLELI K++VTIAP VPPIVL++AK P L 
Sbjct: 247 FHIYSLNSILLCGLRVGAAILIMQKFEISKLLELIEKFKVTIAPFVPPIVLSVAKCPDLH 306

Query: 305 HYDMSSIRIVMSGAAPLGKTLEDAFRAKLPHVILGQGYGMTESGSAMTMSLAFAKEKFEM 364
            YD+SSIR VMSG AP+GK LEDA + KLPH  LGQGYGMTE+G  ++M LAFAKE F +
Sbjct: 307 RYDLSSIRTVMSGGAPMGKELEDAVKEKLPHAKLGQGYGMTEAGPVLSMCLAFAKEPFPI 366

Query: 365 KSGGCGTLMRNSEMKIVNPQTGASLPRNQAGEICIRSPQIMKGYLNDEEATRGIIDKDGW 424
           KSG CGT++RN+EMKIV+P TGASLPRNQ+GEICIR  QIMKGY+ND EAT+G ID+ GW
Sbjct: 367 KSGACGTVVRNAEMKIVDPDTGASLPRNQSGEICIRGKQIMKGYINDAEATKGTIDEGGW 426

Query: 425 LHTGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGRIADVAVIPMKDEVA 484
           LHTGDIGF+D+DDE+FIVDRLKELIKYKGFQVAPAELE++LISH  I D AV+PMKDE A
Sbjct: 427 LHTGDIGFIDNDDELFIVDRLKELIKYKGFQVAPAELESMLISHPNITDAAVVPMKDEAA 486

Query: 485 GEVPVAFIVRSDGSKITEDEIKQYISKQVKPPILALISSFQVVFYKRINRVFFVDSIPKS 544
           GEVPVAF+VRS+GSKITE++IKQYISK             QVVFYKRIN+ FF++ IPK+
Sbjct: 487 GEVPVAFVVRSNGSKITEEDIKQYISK-------------QVVFYKRINKAFFIEEIPKN 542

Query: 545 QSGKILRRQLRALL 559
            SGKILR+ LRA L
Sbjct: 547 PSGKILRKILRAKL 542

BLAST of CcUC06G123190.1 vs. ExPASy Swiss-Prot
Match: O24146 (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1)

HSP 1 Score: 754.2 bits (1946), Expect = 1.1e-216
Identity = 387/560 (69.11%), Postives = 449/560 (80.18%), Query Frame = 0

Query: 5   DDSSPEFIFRSKLPDIPIPTHLPLHSYCFEKISEFKYRPCLINAATGNIYTYDEVHITSR 64
           D    + IFRSKLPDI IP HLPLHSYCFE ISEF  RPCLIN A   IYTY +V + SR
Sbjct: 4   DTKQVDIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVELNSR 63

Query: 65  RVAAGLHKLGIGKGDVIMLLLQNTPQFVLAFLGASYLGAATTPANPFFTPAEIAKQATSS 124
           +VAAGLHK GI   D IM+LL N+P+FV AF+GASYLGA +T ANP FTPAE+ KQA +S
Sbjct: 64  KVAAGLHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKAS 123

Query: 125 GAKLIITQAAFAEKVKKLSQENKAIIKVIFIDDDPPPDGGYHFSSLTDDVAKEEEREMGD 184
            AK+I+TQA    KVK  + EN   +K+I ID    P+G  HFS LT    +  E ++ +
Sbjct: 124 SAKIIVTQACHVNKVKDYAFEND--VKIICIDS--APEGCLHFSVLT----QANEHDIPE 183

Query: 185 VKISPDDVVALPYSSGTTGLPKGVMLTHKALVTSVAQQVDGENPHLYIRSDDVVLCVLPL 244
           V+I PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENP+LYI S+DV+LCVLPL
Sbjct: 184 VEIQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPL 243

Query: 245 FHIYSLNSIMMCSLRVGAAILIVQKFDVNSLLELIPKYRVTIAPIVPPIVLAIAKSPALD 304
           FHIYSLNS+++C LRVGAAILI+QKFD+ S LELI +Y+VTI P VPPIVLAIAKSP +D
Sbjct: 244 FHIYSLNSVLLCGLRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVD 303

Query: 305 HYDMSSIRIVMSGAAPLGKTLEDAFRAKLPHVILGQGYGMTESGSAMTMSLAFAKEKFEM 364
            YD+SS+R VMSGAAPLGK LED  RAK P+  LGQGYGMTE+G  + M LAFAKE FE+
Sbjct: 304 DYDLSSVRTVMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEI 363

Query: 365 KSGGCGTLMRNSEMKIVNPQTGASLPRNQAGEICIRSPQIMKGYLNDEEATRGIIDKDGW 424
           KSG CGT++RN+EMKIV+P+TG SLPRNQ+GEICIR  QIMKGYLND EAT   IDK+GW
Sbjct: 364 KSGACGTVVRNAEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGW 423

Query: 425 LHTGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGRIADVAVIPMKDEVA 484
           L+TGDIG++DDDDE+FIVDRLKELIKYKGFQVAPAELEALL++H  I+D AV+PMKDE A
Sbjct: 424 LYTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQA 483

Query: 485 GEVPVAFIVRSDGSKITEDEIKQYISKQVKPPILALISSFQVVFYKRINRVFFVDSIPKS 544
           GEVPVAF+VRS+GS ITEDE+K +ISK             QV+FYKRI RVFFVD+IPKS
Sbjct: 484 GEVPVAFVVRSNGSTITEDEVKDFISK-------------QVIFYKRIKRVFFVDAIPKS 542

Query: 545 QSGKILRRQLRALLAASIPN 565
            SGKILR+ LRA LAA +PN
Sbjct: 544 PSGKILRKDLRAKLAAGLPN 542

BLAST of CcUC06G123190.1 vs. ExPASy Swiss-Prot
Match: P31684 (4-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1)

HSP 1 Score: 753.4 bits (1944), Expect = 1.8e-216
Identity = 388/564 (68.79%), Postives = 452/564 (80.14%), Query Frame = 0

Query: 1   MAADDDSSPEFIFRSKLPDIPIPTHLPLHSYCFEKISEFKYRPCLINAATGNIYTYDEVH 60
           M  +   S + IFRSKLPDI IP HLPLHSYCFE +SEF  RPCLI+ A   IYTY EV 
Sbjct: 3   MDTETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVE 62

Query: 61  ITSRRVAAGLHKLGIGKGDVIMLLLQNTPQFVLAFLGASYLGAATTPANPFFTPAEIAKQ 120
           +TSR+VA GL+KLGI + D IM+LL N P+FV AF+GASYLGA +T ANP FTPAE+ KQ
Sbjct: 63  LTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 ATSSGAKLIITQAAFAEKVKKLSQENKAIIKVIFIDDDPPPDGGYHFSSLTDDVAKEEER 180
           A +S AK++ITQA FA KVK  + EN   +KVI +D    P+G  HFS L     + +E 
Sbjct: 123 AKASSAKIVITQACFAGKVKDYAIEND--LKVICVDS--VPEGCVHFSEL----IQSDEH 182

Query: 181 EMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKALVTSVAQQVDGENPHLYIRSDDVVLC 240
           E+ DVKI PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGEN +LY+ SDDV++C
Sbjct: 183 EIPDVKIQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMC 242

Query: 241 VLPLFHIYSLNSIMMCSLRVGAAILIVQKFDVNSLLELIPKYRVTIAPIVPPIVLAIAKS 300
           VLPLFHIYSLNS+++C+LRVGAAILI+QKFD+   LELIPK++VTI P VPPIVLAIAKS
Sbjct: 243 VLPLFHIYSLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKS 302

Query: 301 PALDHYDMSSIRIVMSGAAPLGKTLEDAFRAKLPHVILGQGYGMTESGSAMTMSLAFAKE 360
           P +D+YD+SS+R VMSGAAPLGK LEDA RAK P+  LGQGYGMTE+G  + M LAFAKE
Sbjct: 303 PLVDNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKE 362

Query: 361 KFEMKSGGCGTLMRNSEMKIVNPQTGASLPRNQAGEICIRSPQIMKGYLNDEEATRGIID 420
            F++KSG CGT++RN+EMKIV+P TG SLPRNQ GEICIR  QIMKGYLND EAT   I+
Sbjct: 363 PFDIKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIE 422

Query: 421 KDGWLHTGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGRIADVAVIPMK 480
           K+GWLHTGDIGF+DDDDE+FIVDRLKELIKYKGFQVAPAELEALLI+H  I+D AV+PM 
Sbjct: 423 KEGWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMI 482

Query: 481 DEVAGEVPVAFIVRSDGSKITEDEIKQYISKQVKPPILALISSFQVVFYKRINRVFFVDS 540
           DE AGEVPVAF+VRS+GS ITEDE+K +ISK             QV+FYKRI RVFFV++
Sbjct: 483 DEQAGEVPVAFVVRSNGSTITEDEVKDFISK-------------QVIFYKRIKRVFFVET 542

Query: 541 IPKSQSGKILRRQLRALLAASIPN 565
           +PKS SGKILR+ LRA LAA I N
Sbjct: 543 VPKSPSGKILRKDLRARLAAGISN 545

BLAST of CcUC06G123190.1 vs. ExPASy Swiss-Prot
Match: O24145 (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 752.7 bits (1942), Expect = 3.1e-216
Identity = 384/557 (68.94%), Postives = 448/557 (80.43%), Query Frame = 0

Query: 8   SPEFIFRSKLPDIPIPTHLPLHSYCFEKISEFKYRPCLINAATGNIYTYDEVHITSRRVA 67
           S + IFRSKLPDI IP HLPLHSYCFE ISEF  RPCLIN A   IYTY EV +T R+VA
Sbjct: 12  SGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVELTCRKVA 71

Query: 68  AGLHKLGIGKGDVIMLLLQNTPQFVLAFLGASYLGAATTPANPFFTPAEIAKQATSSGAK 127
            GL+KLGI + D IM+LL N+P+FV AF+GASYLGA +T ANP FTPAE+ KQA +S AK
Sbjct: 72  VGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKASSAK 131

Query: 128 LIITQAAFAEKVKKLSQENKAIIKVIFIDDDPPPDGGYHFSSLTDDVAKEEEREMGDVKI 187
           +IITQ+ F  KVK  + EN   +KVI ID    P+G  HFS LT    + +E E+ +VKI
Sbjct: 132 IIITQSCFVGKVKDYASEND--VKVICIDS--APEGCLHFSELT----QSDEHEIPEVKI 191

Query: 188 SPDDVVALPYSSGTTGLPKGVMLTHKALVTSVAQQVDGENPHLYIRSDDVVLCVLPLFHI 247
            PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGEN +LY+ S+DV++CVLPLFHI
Sbjct: 192 QPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHI 251

Query: 248 YSLNSIMMCSLRVGAAILIVQKFDVNSLLELIPKYRVTIAPIVPPIVLAIAKSPALDHYD 307
           YSLNSI++C LRVGAAILI+QKFD+   LELI KY+V+I P VPPIVLAIAKSP +D YD
Sbjct: 252 YSLNSILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYD 311

Query: 308 MSSIRIVMSGAAPLGKTLEDAFRAKLPHVILGQGYGMTESGSAMTMSLAFAKEKFEMKSG 367
           +SS+R VMSGAAPLGK LEDA R K P+  LGQGYGMTE+G  + M LAFAKE F++KSG
Sbjct: 312 LSSVRTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSG 371

Query: 368 GCGTLMRNSEMKIVNPQTGASLPRNQAGEICIRSPQIMKGYLNDEEATRGIIDKDGWLHT 427
            CGT++RN+EMKIV+P TG SLPRNQ GEICIR  QIMKGYLND EAT   IDK+GWLHT
Sbjct: 372 ACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHT 431

Query: 428 GDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGRIADVAVIPMKDEVAGEV 487
           GDIGF+D+DDE+FIVDRLKELIKYKGFQVAPAE+EALL++H  I+D AV+PMKDE AGEV
Sbjct: 432 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEV 491

Query: 488 PVAFIVRSDGSKITEDEIKQYISKQVKPPILALISSFQVVFYKRINRVFFVDSIPKSQSG 547
           PVAF+VRS+GS ITEDE+K +ISK             QV+FYKR+ RVFFV+++PKS SG
Sbjct: 492 PVAFVVRSNGSAITEDEVKDFISK-------------QVIFYKRVKRVFFVETVPKSPSG 547

Query: 548 KILRRQLRALLAASIPN 565
           KILR+ LRA LAA +PN
Sbjct: 552 KILRKDLRARLAAGVPN 547

BLAST of CcUC06G123190.1 vs. ExPASy Swiss-Prot
Match: P31685 (4-coumarate--CoA ligase 2 OS=Solanum tuberosum OX=4113 GN=4CL2 PE=3 SV=1)

HSP 1 Score: 750.7 bits (1937), Expect = 1.2e-215
Identity = 387/564 (68.62%), Postives = 451/564 (79.96%), Query Frame = 0

Query: 1   MAADDDSSPEFIFRSKLPDIPIPTHLPLHSYCFEKISEFKYRPCLINAATGNIYTYDEVH 60
           M  +   S + IFRSKLPDI IP HLPLHSYCFE +SEF  RPCLI+ A   IYTY EV 
Sbjct: 3   MDIETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVE 62

Query: 61  ITSRRVAAGLHKLGIGKGDVIMLLLQNTPQFVLAFLGASYLGAATTPANPFFTPAEIAKQ 120
           +TSR+VA GL+KLGI + D IM+LL N P+FV AF+GASYLGA +T ANP FTPAE+ KQ
Sbjct: 63  LTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 ATSSGAKLIITQAAFAEKVKKLSQENKAIIKVIFIDDDPPPDGGYHFSSLTDDVAKEEER 180
           A +S AK++ITQA FA KVK  + EN   +KVI +D    P+G  HFS L     + +E 
Sbjct: 123 AKASSAKIVITQACFAGKVKDYAIEND--LKVICVDS--APEGCVHFSEL----IQSDEH 182

Query: 181 EMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKALVTSVAQQVDGENPHLYIRSDDVVLC 240
           E+ DVKI PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGEN +LY+ SDDV++C
Sbjct: 183 EIPDVKIQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMC 242

Query: 241 VLPLFHIYSLNSIMMCSLRVGAAILIVQKFDVNSLLELIPKYRVTIAPIVPPIVLAIAKS 300
           VLPLFHIYSLNS+++C+LRVGAAILI+QKFD+   LELIPK++VTI P VPPIVLAIAKS
Sbjct: 243 VLPLFHIYSLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKS 302

Query: 301 PALDHYDMSSIRIVMSGAAPLGKTLEDAFRAKLPHVILGQGYGMTESGSAMTMSLAFAKE 360
           P + +YD+SS+R VMSGAAPLGK LEDA RAK P+  LGQGYGMTE+G  + M LAFAKE
Sbjct: 303 PLVHNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKE 362

Query: 361 KFEMKSGGCGTLMRNSEMKIVNPQTGASLPRNQAGEICIRSPQIMKGYLNDEEATRGIID 420
            F++KSG CGT++RN+EMKIV+P TG SLPRNQ GEICIR  QIMKGYLND EAT   I+
Sbjct: 363 PFDIKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIE 422

Query: 421 KDGWLHTGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGRIADVAVIPMK 480
           K+GWLHTGDIGF+DDDDE+FIVDRLKELIKYKGFQVAPAELEALLI+H  I+D AV+PM 
Sbjct: 423 KEGWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMI 482

Query: 481 DEVAGEVPVAFIVRSDGSKITEDEIKQYISKQVKPPILALISSFQVVFYKRINRVFFVDS 540
           DE AGEVPVAF+VRS+GS ITEDE+K +ISK             QV+FYKRI RVFFV++
Sbjct: 483 DEQAGEVPVAFVVRSNGSTITEDEVKDFISK-------------QVIFYKRIKRVFFVET 542

Query: 541 IPKSQSGKILRRQLRALLAASIPN 565
           +PKS SGKILR+ LRA LAA I N
Sbjct: 543 VPKSPSGKILRKDLRARLAAGISN 545

BLAST of CcUC06G123190.1 vs. ExPASy TrEMBL
Match: A0A5D3CN41 (4-coumarate--CoA ligase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold152G00670 PE=4 SV=1)

HSP 1 Score: 931.8 bits (2407), Expect = 1.4e-267
Identity = 482/563 (85.61%), Postives = 509/563 (90.41%), Query Frame = 0

Query: 1   MAADDDSSPEFIFRSKLPDIPIPTHLPLHSYCFEKISEFKYRPCLINAATGNIYTYDEVH 60
           MAA D SSPEF+FRSKLP+IPI THLPLH+YCFE ISEFK RPCLINAATG+ YTY EV 
Sbjct: 1   MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ 60

Query: 61  ITSRRVAAGLHKLGIGKGDVIMLLLQNTPQFVLAFLGASYLGAATTPANPFFTPAEIAKQ 120
            TSRRVAAGLHKLGIGKGDVIMLLLQNTP+FVLAFL ASYLGAA T ANPFF PAEIAKQ
Sbjct: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ 120

Query: 121 ATSSGAKLIITQAAFAEKVKKLSQENKAIIKVIFI-DDDPPPDGGYHFSSLTDDVAKEEE 180
           A SSGAK+IITQ+AFAEKVKKLSQ ++ IIKVIFI DDDPP  GGY FSSL DDVAKEEE
Sbjct: 121 AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEE 180

Query: 181 REMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKALVTSVAQQVDGENPHLYIRSDDVVL 240
            EMGDVKISPDDVVALPYSSGTTGLPKGVMLTHK  VTSVAQQVDGENPHLYIRSDDVVL
Sbjct: 181 LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL 240

Query: 241 CVLPLFHIYSLNSIMMCSLRVGAAILIVQKFDVNSLLELIPKYRVTIAPIVPPIVLAIAK 300
           C+LPLFHIYSLNSIMMCSLRVGAAILIVQKFD+NSLLEL+PKY+VT AP+VPPIVLAIAK
Sbjct: 241 CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK 300

Query: 301 SPALDHYDMSSIRIVMSGAAPLGKTLEDAFRAKLPHVILGQGYGMTESGSAMTMSLAFAK 360
           SPA+DH+DMSS+R+V+SGAAPLGK LEDAFRAKLPHVILGQGYGMTE+GS MTMSLAFAK
Sbjct: 301 SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK 360

Query: 361 EKFEMKSGGCGTLMRNSEMKIVNPQTGASLPRNQAGEICIRSPQIMKGYLNDEEATRGII 420
           E F +KSGGCGT+MRNSEMKIV PQTG SLPRN+ GEICIRS Q+MKGYLNDEEAT+ II
Sbjct: 361 EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII 420

Query: 421 DKDGWLHTGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGRIADVAVIPM 480
           DKDGWLHTGDIG+VDDDDE+FIVDRLKELIKYKGFQVAPAELEALLISHG IAD AVIPM
Sbjct: 421 DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 480

Query: 481 KDEVAGEVPVAFIVRSDGSKITEDEIKQYISKQVKPPILALISSFQVVFYKRINRVFFVD 540
            DEVAGEVPV FIVR DGS ITEDEIKQYISK             QVVFYKRINRVFFVD
Sbjct: 481 NDEVAGEVPVTFIVRFDGSNITEDEIKQYISK-------------QVVFYKRINRVFFVD 540

Query: 541 SIPKSQSGKILRRQLRALLAASI 563
           SIPKS SGKILRRQLRALLAASI
Sbjct: 541 SIPKSPSGKILRRQLRALLAASI 550

BLAST of CcUC06G123190.1 vs. ExPASy TrEMBL
Match: A0A1S3BWP0 (4-coumarate--CoA ligase 1-like OS=Cucumis melo OX=3656 GN=LOC103494274 PE=4 SV=1)

HSP 1 Score: 931.8 bits (2407), Expect = 1.4e-267
Identity = 482/563 (85.61%), Postives = 509/563 (90.41%), Query Frame = 0

Query: 1   MAADDDSSPEFIFRSKLPDIPIPTHLPLHSYCFEKISEFKYRPCLINAATGNIYTYDEVH 60
           MAA D SSPEF+FRSKLP+IPI THLPLH+YCFE ISEFK RPCLINAATG+ YTY EV 
Sbjct: 1   MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ 60

Query: 61  ITSRRVAAGLHKLGIGKGDVIMLLLQNTPQFVLAFLGASYLGAATTPANPFFTPAEIAKQ 120
            TSRRVAAGLHKLGIGKGDVIMLLLQNTP+FVLAFL ASYLGAA T ANPFF PAEIAKQ
Sbjct: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ 120

Query: 121 ATSSGAKLIITQAAFAEKVKKLSQENKAIIKVIFI-DDDPPPDGGYHFSSLTDDVAKEEE 180
           A SSGAK+IITQ+AFAEKVKKLSQ ++ IIKVIFI DDDPP  GGY FSSL DDVAKEEE
Sbjct: 121 AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEE 180

Query: 181 REMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKALVTSVAQQVDGENPHLYIRSDDVVL 240
            EMGDVKISPDDVVALPYSSGTTGLPKGVMLTHK  VTSVAQQVDGENPHLYIRSDDVVL
Sbjct: 181 LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL 240

Query: 241 CVLPLFHIYSLNSIMMCSLRVGAAILIVQKFDVNSLLELIPKYRVTIAPIVPPIVLAIAK 300
           C+LPLFHIYSLNSIMMCSLRVGAAILIVQKFD+NSLLEL+PKY+VT AP+VPPIVLAIAK
Sbjct: 241 CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK 300

Query: 301 SPALDHYDMSSIRIVMSGAAPLGKTLEDAFRAKLPHVILGQGYGMTESGSAMTMSLAFAK 360
           SPA+DH+DMSS+R+V+SGAAPLGK LEDAFRAKLPHVILGQGYGMTE+GS MTMSLAFAK
Sbjct: 301 SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK 360

Query: 361 EKFEMKSGGCGTLMRNSEMKIVNPQTGASLPRNQAGEICIRSPQIMKGYLNDEEATRGII 420
           E F +KSGGCGT+MRNSEMKIV PQTG SLPRN+ GEICIRS Q+MKGYLNDEEAT+ II
Sbjct: 361 EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII 420

Query: 421 DKDGWLHTGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGRIADVAVIPM 480
           DKDGWLHTGDIG+VDDDDE+FIVDRLKELIKYKGFQVAPAELEALLISHG IAD AVIPM
Sbjct: 421 DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 480

Query: 481 KDEVAGEVPVAFIVRSDGSKITEDEIKQYISKQVKPPILALISSFQVVFYKRINRVFFVD 540
            DEVAGEVPV FIVR DGS ITEDEIKQYISK             QVVFYKRINRVFFVD
Sbjct: 481 NDEVAGEVPVTFIVRFDGSNITEDEIKQYISK-------------QVVFYKRINRVFFVD 540

Query: 541 SIPKSQSGKILRRQLRALLAASI 563
           SIPKS SGKILRRQLRALLAASI
Sbjct: 541 SIPKSPSGKILRRQLRALLAASI 550

BLAST of CcUC06G123190.1 vs. ExPASy TrEMBL
Match: A0A5A7UT30 (4-coumarate--CoA ligase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G006230 PE=4 SV=1)

HSP 1 Score: 931.0 bits (2405), Expect = 2.3e-267
Identity = 482/565 (85.31%), Postives = 509/565 (90.09%), Query Frame = 0

Query: 1   MAADDDSSPEFIFRSKLPDIPIPTHLPLHSYCFEKISEFKYRPCLINAATGNIYTYDEVH 60
           MAA D SSPEF+FRSKLP+IPI THLPLH+YCFE ISEFK RPCLINAATG+ YTY EV 
Sbjct: 1   MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ 60

Query: 61  ITSRRVAAGLHKLGIGKGDVIMLLLQNTPQFVLAFLGASYLGAATTPANPFFTPAEIAKQ 120
             SRRVAAGLHKLGIGKGDVIMLLLQNTP+FVLAFL ASYLGAA T ANPFF PAEIAKQ
Sbjct: 61  AISRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ 120

Query: 121 ATSSGAKLIITQAAFAEKVKKLSQENKAIIKVIFI-DDDPPPDGGYHFSSLTDDVAKEEE 180
           A SSGAK+IITQ+AFAEKVKKLSQ ++ IIKVIFI DDDPP  GGY FSSL DDVAKEEE
Sbjct: 121 AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEE 180

Query: 181 REMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKALVTSVAQQVDGENPHLYIRSDDVVL 240
            EMGDVKISPDDVVALPYSSGTTGLPKGVMLTHK  VTSVAQQVDGENPHLYIRSDDVVL
Sbjct: 181 LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL 240

Query: 241 CVLPLFHIYSLNSIMMCSLRVGAAILIVQKFDVNSLLELIPKYRVTIAPIVPPIVLAIAK 300
           C+LPLFHIYSLNSIMMCSLRVGAAILIVQKFD+NSLLEL+PKY+VT AP+VPPIVLAIAK
Sbjct: 241 CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK 300

Query: 301 SPALDHYDMSSIRIVMSGAAPLGKTLEDAFRAKLPHVILGQGYGMTESGSAMTMSLAFAK 360
           SPA+DH+DMSS+R+V+SGAAPLGK LEDAFRAKLPHVILGQGYGMTE+GS MTMSLAFAK
Sbjct: 301 SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK 360

Query: 361 EKFEMKSGGCGTLMRNSEMKIVNPQTGASLPRNQAGEICIRSPQIMKGYLNDEEATRGII 420
           E F +KSGGCGT+MRNSEMKIV PQTG SLPRN+ GEICIRS Q+MKGYLNDEEAT+ II
Sbjct: 361 EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII 420

Query: 421 DKDGWLHTGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGRIADVAVIPM 480
           DKDGWLHTGDIG+VDDDDE+FIVDRLKELIKYKGFQVAPAELEALLISHG IAD AVIPM
Sbjct: 421 DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 480

Query: 481 KDEVAGEVPVAFIVRSDGSKITEDEIKQYISKQVKPPILALISSFQVVFYKRINRVFFVD 540
            DEVAGEVPV FIVR DGS ITEDEIKQYISK             QVVFYKRINRVFFVD
Sbjct: 481 NDEVAGEVPVTFIVRFDGSNITEDEIKQYISK-------------QVVFYKRINRVFFVD 540

Query: 541 SIPKSQSGKILRRQLRALLAASIPN 565
           SIPKS SGKILRRQLRALLAASI N
Sbjct: 541 SIPKSPSGKILRRQLRALLAASILN 552

BLAST of CcUC06G123190.1 vs. ExPASy TrEMBL
Match: A0A0A0LWT6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G423020 PE=4 SV=1)

HSP 1 Score: 914.8 bits (2363), Expect = 1.7e-262
Identity = 474/562 (84.34%), Postives = 506/562 (90.04%), Query Frame = 0

Query: 1   MAADDDSSPEFIFRSKLPDIPIPTHLPLHSYCFEKISEFKYRPCLINAATGNIYTYDEVH 60
           MAA D SSPEFIFRSKLP+IPI THLPLH+YCFE ISEFK+RPCLINAATG+IYTY EV 
Sbjct: 1   MAAADASSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKHRPCLINAATGHIYTYGEVQ 60

Query: 61  ITSRRVAAGLHKLGIGKGDVIMLLLQNTPQFVLAFLGASYLGAATTPANPFFTPAEIAKQ 120
            TSRRVAAGLHKLGIGKGDVIMLLLQNTP+FVLAFLGASYLGAA T ANPFF  +EIAKQ
Sbjct: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLGASYLGAAATMANPFFMQSEIAKQ 120

Query: 121 ATSSGAKLIITQAAFAEKVKKLSQENKAIIKVIFIDDDPPPDGGYHFSSLTDDVAKEEER 180
           A SSGAK+IITQAAFAEKVK LSQEN+ IIKVIFIDD+ PP     FSSL +DVAKEEE 
Sbjct: 121 AVSSGAKVIITQAAFAEKVKNLSQENEMIIKVIFIDDNDPP----QFSSLIEDVAKEEEL 180

Query: 181 EMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKALVTSVAQQVDGENPHLYIRSDDVVLC 240
           EMGDVKISP+DVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPHL+IRSDDVVLC
Sbjct: 181 EMGDVKISPEDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLC 240

Query: 241 VLPLFHIYSLNSIMMCSLRVGAAILIVQKFDVNSLLELIPKYRVTIAPIVPPIVLAIAKS 300
           +LPLFHIYSLNSIMMC+LRVGAAIL+VQKFDVNSL+EL+ KY+VT AP VPPIVLAIAKS
Sbjct: 241 LLPLFHIYSLNSIMMCALRVGAAILLVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKS 300

Query: 301 PALDHYDMSSIRIVMSGAAPLGKTLEDAFRAKLPHVILGQGYGMTESGSAMTMSLAFAKE 360
           PA+DH+DMSS+RIV+SGAAPLGK LEDAFRAKLPHVILGQGYGMTE+GS MTMSLAF KE
Sbjct: 301 PAVDHFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKE 360

Query: 361 KFEMKSGGCGTLMRNSEMKIVNPQTGASLPRNQAGEICIRSPQIMKGYLNDEEATRGIID 420
            F +KSGGCGT+MRNSEMKI+N QTG SLPRNQ GEICIRS Q+MKGYLNDE+AT+ IID
Sbjct: 361 GFGIKSGGCGTIMRNSEMKILNLQTGESLPRNQTGEICIRSSQMMKGYLNDEDATKAIID 420

Query: 421 KDGWLHTGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGRIADVAVIPMK 480
           +DGWLHTGDIGFVDDDDE+FIVDRLKELIKYKGFQVAPAELEALLISH  IAD AVIPM 
Sbjct: 421 EDGWLHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMN 480

Query: 481 DEVAGEVPVAFIVRSDGSKITEDEIKQYISKQVKPPILALISSFQVVFYKRINRVFFVDS 540
           DEVAGEVPVAFIVR DGS ITEDEIKQ+ISK             QVVFYKRINRVFFVDS
Sbjct: 481 DEVAGEVPVAFIVRFDGSNITEDEIKQFISK-------------QVVFYKRINRVFFVDS 540

Query: 541 IPKSQSGKILRRQLRALLAASI 563
           IPKS SGKILRRQLRALLAASI
Sbjct: 541 IPKSPSGKILRRQLRALLAASI 545

BLAST of CcUC06G123190.1 vs. ExPASy TrEMBL
Match: A0A6J1E5C5 (4-coumarate--CoA ligase 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429639 PE=4 SV=1)

HSP 1 Score: 880.2 bits (2273), Expect = 4.7e-252
Identity = 456/559 (81.57%), Postives = 494/559 (88.37%), Query Frame = 0

Query: 6   DSSPEFIFRSKLPDIPIPTHLPLHSYCFEKISEFKYRPCLINAATGNIYTYDEVHITSRR 65
           DSSPEFIFRSKLPD+ IP HLPLH+YCFE+IS+FK+ PCLINAATG+IYTY EVH+T+RR
Sbjct: 5   DSSPEFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHVTARR 64

Query: 66  VAAGLHKLGIGKGDVIMLLLQNTPQFVLAFLGASYLGAATTPANPFFTPAEIAKQATSSG 125
           VAAG HKLGIGKGDVIMLLLQNTPQFV AFLGAS+  A  T ANPFFTPAEIAKQA SSG
Sbjct: 65  VAAGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQAASSG 124

Query: 126 AKLIITQAAFAEKVKKLSQENKAIIKVIFIDDDPPPDGGYHFSSLTDDVAKEEEREMGDV 185
           AKLIITQAAFAEKVKKL QEN+ I+KVIFID   PP+GG HF+SLTD+ AKEE  EM DV
Sbjct: 125 AKLIITQAAFAEKVKKLPQENQGILKVIFIDS--PPEGGCHFTSLTDE-AKEE--EMADV 184

Query: 186 KISPDDVVALPYSSGTTGLPKGVMLTHKALVTSVAQQVDGENPHLYIRSDDVVLCVLPLF 245
           K SPDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPH +IRS+DVVLCVLPLF
Sbjct: 185 KFSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLF 244

Query: 246 HIYSLNSIMMCSLRVGAAILIVQKFDVNSLLELIPKYRVTIAPIVPPIVLAIAKSPALDH 305
           HIYSL+SI+M SLRVGAAILIVQKFD+NSL+ L+PKYRVTIA +VPPIVLA+ KSP +DH
Sbjct: 245 HIYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAMVKSPVVDH 304

Query: 306 YDMSSIRIVMSGAAPLGKTLEDAFRAKLPHVILGQGYGMTESGSAMTMSLAFAKEKFEMK 365
            DMSSIRIV+SGAAPLGK LEDAFRAKLPH IL QGYGMTESGS +TMSLA A+EKF +K
Sbjct: 305 SDMSSIRIVLSGAAPLGKNLEDAFRAKLPHAILAQGYGMTESGSVLTMSLALAREKFAIK 364

Query: 366 SGGCGTLMRNSEMKIVNPQTGASLPRNQAGEICIRSPQIMKGYLNDEEATRGIIDKDGWL 425
           SG CGTLMRN+EMKIVNP+ G SL RNQ GEI +RSPQ+MKGYLNDEEAT+G+ID+ GWL
Sbjct: 365 SGACGTLMRNAEMKIVNPENGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGMIDESGWL 424

Query: 426 HTGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGRIADVAVIPMKDEVAG 485
           HTGD+G VDDDDE+FIVDRLKELIKYKGFQVAPAELE LLIS+  I DVAVIPMKDEVAG
Sbjct: 425 HTGDMGLVDDDDELFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAG 484

Query: 486 EVPVAFIVRSDGSKITEDEIKQYISKQVKPPILALISSFQVVFYKRINRVFFVDSIPKSQ 545
           EVPVAFIVRSDGS ITEDEIKQYISK             QVVFYKRINRVFFVDSIPKS 
Sbjct: 485 EVPVAFIVRSDGSNITEDEIKQYISK-------------QVVFYKRINRVFFVDSIPKSP 544

Query: 546 SGKILRRQLRALLAASIPN 565
           SGKILRRQLRALL ASIPN
Sbjct: 545 SGKILRRQLRALLPASIPN 545

BLAST of CcUC06G123190.1 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 712.6 bits (1838), Expect = 2.5e-205
Identity = 364/558 (65.23%), Postives = 439/558 (78.67%), Query Frame = 0

Query: 5   DDSSPEFIFRSKLPDIPIPTHLPLHSYCFEKISEFKYRPCLINAATGNIYTYDEVHITSR 64
           ++++ + IFRSKLPDI IP HL LH Y F+ ISEF  +PCLIN  TG++YTY +VH+ SR
Sbjct: 17  NNNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISR 76

Query: 65  RVAAGLHKLGIGKGDVIMLLLQNTPQFVLAFLGASYLGAATTPANPFFTPAEIAKQATSS 124
           ++AA  HKLG+ + DV+MLLL N P+FVL+FL AS+ GA  T ANPFFTPAEIAKQA +S
Sbjct: 77  QIAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKAS 136

Query: 125 GAKLIITQAAFAEKVKKLSQENKAIIKVIFIDDD---PPPDGGYHFSSLTDDVAKEEERE 184
             KLIIT+A + +K+K L  ++  +I  + IDD+   P P+G   F+ LT    +  E  
Sbjct: 137 NTKLIITEARYVDKIKPLQNDDGVVI--VCIDDNESVPIPEGCLRFTELTQSTTEASE-V 196

Query: 185 MGDVKISPDDVVALPYSSGTTGLPKGVMLTHKALVTSVAQQVDGENPHLYIRSDDVVLCV 244
           +  V+ISPDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENP+LY  SDDV+LCV
Sbjct: 197 IDSVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCV 256

Query: 245 LPLFHIYSLNSIMMCSLRVGAAILIVQKFDVNSLLELIPKYRVTIAPIVPPIVLAIAKSP 304
           LP+FHIY+LNSIM+C LRVGAAILI+ KF++N LLELI + +VT+AP+VPPIVLAIAKS 
Sbjct: 257 LPMFHIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSS 316

Query: 305 ALDHYDMSSIRIVMSGAAPLGKTLEDAFRAKLPHVILGQGYGMTESGSAMTMSLAFAKEK 364
             + YD+SSIR+V SGAAPLGK LEDA  AK P+  LGQGYGMTE+G  + MSL FAKE 
Sbjct: 317 ETEKYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEP 376

Query: 365 FEMKSGGCGTLMRNSEMKIVNPQTGASLPRNQAGEICIRSPQIMKGYLNDEEATRGIIDK 424
           F +KSG CGT++RN+EMKIV+P TG SL RNQ GEICIR  QIMKGYLN+  AT   IDK
Sbjct: 377 FPVKSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDK 436

Query: 425 DGWLHTGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGRIADVAVIPMKD 484
           DGWLHTGDIG +DDDDE+FIVDRLKELIKYKGFQVAPAELEALLI H  I DVAV+ MK+
Sbjct: 437 DGWLHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKE 496

Query: 485 EVAGEVPVAFIVRSDGSKITEDEIKQYISKQVKPPILALISSFQVVFYKRINRVFFVDSI 544
           E AGEVPVAF+V+S  S+++ED++KQ++SK             QVVFYKRIN+VFF +SI
Sbjct: 497 EAAGEVPVAFVVKSKDSELSEDDVKQFVSK-------------QVVFYKRINKVFFTESI 556

Query: 545 PKSQSGKILRRQLRALLA 560
           PK+ SGKILR+ LRA LA
Sbjct: 557 PKAPSGKILRKDLRAKLA 558

BLAST of CcUC06G123190.1 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 710.7 bits (1833), Expect = 9.5e-205
Identity = 362/557 (64.99%), Postives = 435/557 (78.10%), Query Frame = 0

Query: 8   SPEFIFRSKLPDIPIPTHLPLHSYCFEKISEFKYRPCLINAATGNIYTYDEVHITSRRVA 67
           S + IFRS+LPDI IP HLPLH Y FE ISEF  +PCLIN  TG +YTY +VH+TSR++A
Sbjct: 18  SNDVIFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 77

Query: 68  AGLHKLGIGKGDVIMLLLQNTPQFVLAFLGASYLGAATTPANPFFTPAEIAKQATSSGAK 127
           AGLH LG+ + DV+M+LL N+P+ VL FL AS++GA TT ANPFFTPAEI+KQA +S AK
Sbjct: 78  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 137

Query: 128 LIITQAAFAEKVKKLSQENKAIIKVIFIDDDPPPDGGYHFSSLTDDVAKEEEREMGDVKI 187
           LI+TQ+ + +K+K L  +    + ++  D D  P+    FS LT   ++E   +    KI
Sbjct: 138 LIVTQSRYVDKIKNLQNDG---VLIVTTDSDAIPENCLRFSELTQ--SEEPRVDSIPEKI 197

Query: 188 SPDDVVALPYSSGTTGLPKGVMLTHKALVTSVAQQVDGENPHLYIRSDDVVLCVLPLFHI 247
           SP+DVVALP+SSGTTGLPKGVMLTHK LVTSVAQQVDGENP+LY   DDV+LCVLP+FHI
Sbjct: 198 SPEDVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHI 257

Query: 248 YSLNSIMMCSLRVGAAILIVQKFDVNSLLELIPKYRVTIAPIVPPIVLAIAKSPALDHYD 307
           Y+LNSIM+CSLRVGA ILI+ KF++  LLE I + +VT+A +VPPIVLAIAKSP  + YD
Sbjct: 258 YALNSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYD 317

Query: 308 MSSIRIVMSGAAPLGKTLEDAFRAKLPHVILGQGYGMTESGSAMTMSLAFAKEKFEMKSG 367
           +SS+R+V SGAAPLGK LEDA  AK P+  LGQGYGMTE+G  + MSL FAKE F +KSG
Sbjct: 318 LSSVRMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSG 377

Query: 368 GCGTLMRNSEMKIVNPQTGASLPRNQAGEICIRSPQIMKGYLNDEEATRGIIDKDGWLHT 427
            CGT++RN+EMKI++P TG SLPRN+ GEICIR  QIMKGYLND  AT   IDKDGWLHT
Sbjct: 378 ACGTVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHT 437

Query: 428 GDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGRIADVAVIPMKDEVAGEV 487
           GD+GF+DDDDE+FIVDRLKELIKYKGFQVAPAELE+LLI H  I DVAV+ MK+E AGEV
Sbjct: 438 GDVGFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEV 497

Query: 488 PVAFIVRSDGSKITEDEIKQYISKQVKPPILALISSFQVVFYKRINRVFFVDSIPKSQSG 547
           PVAF+VRS  S I+EDEIKQ++SK             QVVFYKRIN+VFF DSIPK+ SG
Sbjct: 498 PVAFVVRSKDSNISEDEIKQFVSK-------------QVVFYKRINKVFFTDSIPKAPSG 556

Query: 548 KILRRQLRALLAASIPN 565
           KILR+ LRA LA  + N
Sbjct: 558 KILRKDLRARLANGLMN 556

BLAST of CcUC06G123190.1 vs. TAIR 10
Match: AT1G51680.3 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 676.8 bits (1745), Expect = 1.5e-194
Identity = 341/517 (65.96%), Postives = 413/517 (79.88%), Query Frame = 0

Query: 5   DDSSPEFIFRSKLPDIPIPTHLPLHSYCFEKISEFKYRPCLINAATGNIYTYDEVHITSR 64
           ++++ + IFRSKLPDI IP HL LH Y F+ ISEF  +PCLIN  TG++YTY +VH+ SR
Sbjct: 17  NNNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISR 76

Query: 65  RVAAGLHKLGIGKGDVIMLLLQNTPQFVLAFLGASYLGAATTPANPFFTPAEIAKQATSS 124
           ++AA  HKLG+ + DV+MLLL N P+FVL+FL AS+ GA  T ANPFFTPAEIAKQA +S
Sbjct: 77  QIAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKAS 136

Query: 125 GAKLIITQAAFAEKVKKLSQENKAIIKVIFIDDD---PPPDGGYHFSSLTDDVAKEEERE 184
             KLIIT+A + +K+K L  ++  +I  + IDD+   P P+G   F+ LT    +  E  
Sbjct: 137 NTKLIITEARYVDKIKPLQNDDGVVI--VCIDDNESVPIPEGCLRFTELTQSTTEASE-V 196

Query: 185 MGDVKISPDDVVALPYSSGTTGLPKGVMLTHKALVTSVAQQVDGENPHLYIRSDDVVLCV 244
           +  V+ISPDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENP+LY  SDDV+LCV
Sbjct: 197 IDSVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCV 256

Query: 245 LPLFHIYSLNSIMMCSLRVGAAILIVQKFDVNSLLELIPKYRVTIAPIVPPIVLAIAKSP 304
           LP+FHIY+LNSIM+C LRVGAAILI+ KF++N LLELI + +VT+AP+VPPIVLAIAKS 
Sbjct: 257 LPMFHIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSS 316

Query: 305 ALDHYDMSSIRIVMSGAAPLGKTLEDAFRAKLPHVILGQGYGMTESGSAMTMSLAFAKEK 364
             + YD+SSIR+V SGAAPLGK LEDA  AK P+  LGQGYGMTE+G  + MSL FAKE 
Sbjct: 317 ETEKYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEP 376

Query: 365 FEMKSGGCGTLMRNSEMKIVNPQTGASLPRNQAGEICIRSPQIMKGYLNDEEATRGIIDK 424
           F +KSG CGT++RN+EMKIV+P TG SL RNQ GEICIR  QIMKGYLN+  AT   IDK
Sbjct: 377 FPVKSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDK 436

Query: 425 DGWLHTGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGRIADVAVIPMKD 484
           DGWLHTGDIG +DDDDE+FIVDRLKELIKYKGFQVAPAELEALLI H  I DVAV+ MK+
Sbjct: 437 DGWLHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKE 496

Query: 485 EVAGEVPVAFIVRSDGSKITEDEIKQYISKQVKPPIL 519
           E AGEVPVAF+V+S  S+++ED++KQ++SKQVK  +L
Sbjct: 497 EAAGEVPVAFVVKSKDSELSEDDVKQFVSKQVKSCVL 530

BLAST of CcUC06G123190.1 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 661.4 bits (1705), Expect = 6.6e-190
Identity = 335/548 (61.13%), Postives = 428/548 (78.10%), Query Frame = 0

Query: 12  IFRSKLPDIPIPTHLPLHSYCFEKISEFKYRPCLINAATGNIYTYDEVHITSRRVAAGLH 71
           IFRSKLPDI IP HLPLH+YCFEK+S    +PCLI  +TG  YTY E H+  RRVA+GL+
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 72  KLGIGKGDVIMLLLQNTPQFVLAFLGASYLGAATTPANPFFTPAEIAKQATSSGAKLIIT 131
           KLGI KGDVIM+LLQN+ +FV +F+GAS +GA +T ANPF+T  E+ KQ  SSGAKLIIT
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 132 QAAFAEKVKKLSQENKAIIKVIFIDDDPPPDGGYHFSSL-TDDVAKEEEREMGDVKISPD 191
            + + +K+K L  EN  +I      D+P P+    FS+L TDD   E       V I  D
Sbjct: 154 HSQYVDKLKNLG-ENLTLITT----DEPTPENCLPFSTLITDD---ETNPFQETVDIGGD 213

Query: 192 DVVALPYSSGTTGLPKGVMLTHKALVTSVAQQVDGENPHLYIRSDDVVLCVLPLFHIYSL 251
           D  ALP+SSGTTGLPKGV+LTHK+L+TSVAQQVDG+NP+LY++S+DV+LCVLPLFHIYSL
Sbjct: 214 DAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSL 273

Query: 252 NSIMMCSLRVGAAILIVQKFDVNSLLELIPKYRVTIAPIVPPIVLAIAKSPALDHYDMSS 311
           NS+++ SLR GA +L++ KF++ +LL+LI ++RVTIA +VPP+V+A+AK+P ++ YD+SS
Sbjct: 274 NSVLLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSS 333

Query: 312 IRIVMSGAAPLGKTLEDAFRAKLPHVILGQGYGMTESGSAMTMSLAFAKEKFEMKSGGCG 371
           +R V+SGAAPLGK L+D+ R +LP  ILGQGYGMTE+G  ++MSL FAKE    KSG CG
Sbjct: 334 VRFVLSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCG 393

Query: 372 TLMRNSEMKIVNPQTGASLPRNQAGEICIRSPQIMKGYLNDEEATRGIIDKDGWLHTGDI 431
           T++RN+E+K+V+ +T  SL  NQ GEICIR  QIMK YLND EAT   ID++GWLHTGDI
Sbjct: 394 TVVRNAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDI 453

Query: 432 GFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGRIADVAVIPMKDEVAGEVPVA 491
           G+VD+DDE+FIVDRLKE+IK+KGFQV PAELE+LLI+H  IAD AV+P  DEVAGEVPVA
Sbjct: 454 GYVDEDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVA 513

Query: 492 FIVRSDGSKITEDEIKQYISKQVKPPILALISSFQVVFYKRINRVFFVDSIPKSQSGKIL 551
           F+VRS+G+ ITE+++K+Y++K             QVVFYKR+++VFFV SIPKS SGKIL
Sbjct: 514 FVVRSNGNDITEEDVKEYVAK-------------QVVFYKRLHKVFFVASIPKSPSGKIL 560

Query: 552 RRQLRALL 559
           R+ L+A L
Sbjct: 574 RKDLKAKL 560

BLAST of CcUC06G123190.1 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 636.3 bits (1640), Expect = 2.3e-182
Identity = 326/566 (57.60%), Postives = 421/566 (74.38%), Query Frame = 0

Query: 4   DDDSSPEFIFRSKLPDIPIPTHLPLHSYCFEKIS----EFKYRPCLINAATGNIYTYDEV 63
           +++ S +FIFRSKLPDI IP HLPL  Y F++ S          C+I+ ATG I TY +V
Sbjct: 20  EEEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADV 79

Query: 64  HITSRRVAAGLHKLGIGKGDVIMLLLQNTPQFVLAFLGASYLGAATTPANPFFTPAEIAK 123
               RR+AAG+H+LGI  GDV+MLLL N+P+F L+FL  +YLGA +T ANPF+T  EIAK
Sbjct: 80  QTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAK 139

Query: 124 QATSSGAKLIITQAAFAEKVKKLSQENKAIIKVIFIDDD-------PPPDGGYHFSSLTD 183
           QA +S AK+IIT+    +K+  L  +    + ++ +DDD          DG   F+ LT 
Sbjct: 140 QAKASAAKMIITKKCLVDKLTNLKNDG---VLIVCLDDDGDNGVVSSSDDGCVSFTELT- 199

Query: 184 DVAKEEEREMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKALVTSVAQQVDGENPHLYI 243
              + +E E+   KISP+D VA+PYSSGTTGLPKGVM+THK LVTS+AQ+VDGENP+L  
Sbjct: 200 ---QADETELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNF 259

Query: 244 RSDDVVLCVLPLFHIYSLNSIMMCSLRVGAAILIVQKFDVNSLLELIPKYRVTIAPIVPP 303
            ++DV+LC LP+FHIY+L+++M+ ++R GAA+LIV +F++N ++ELI +Y+VT+ P+ PP
Sbjct: 260 TANDVILCFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPP 319

Query: 304 IVLAIAKSPALDHYDMSSIRIVMSGAAPLGKTLEDAFRAKLPHVILGQGYGMTESGSAMT 363
           +VLA  KSP  + YD+SS+RI++SGAA L K LEDA R K P+ I GQGYGMTESG+ + 
Sbjct: 320 VVLAFIKSPETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGT-VA 379

Query: 364 MSLAFAKEKFEMKSGGCGTLMRNSEMKIVNPQTGASLPRNQAGEICIRSPQIMKGYLNDE 423
            SLAFAK  F+ KSG CGT++RN+EMK+V+ +TG SLPRN++GEIC+R  Q+MKGYLND 
Sbjct: 380 KSLAFAKNPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDP 439

Query: 424 EATRGIIDKDGWLHTGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGRIA 483
           EAT   IDKDGWLHTGDIGFVDDDDE+FIVDRLKELIK+KG+QVAPAELEALLISH  I 
Sbjct: 440 EATARTIDKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSID 499

Query: 484 DVAVIPMKDEVAGEVPVAFIVRSDGSKITEDEIKQYISKQVKPPILALISSFQVVFYKRI 543
           D AV+ MKDEVA EVPVAF+ RS GS++TED++K Y++K             QVV YKRI
Sbjct: 500 DAAVVAMKDEVADEVPVAFVARSQGSQLTEDDVKSYVNK-------------QVVHYKRI 559

Query: 544 NRVFFVDSIPKSQSGKILRRQLRALL 559
             VFF++ IPK+ SGKILR+ LRA L
Sbjct: 560 KMVFFIEVIPKAVSGKILRKDLRAKL 564

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038878634.11.8e-27487.154-coumarate--CoA ligase 1-like [Benincasa hispida][more]
XP_008453615.12.8e-26785.61PREDICTED: 4-coumarate--CoA ligase 1-like [Cucumis melo] >TYK11846.1 4-coumarate... [more]
KAA0058290.14.8e-26785.314-coumarate--CoA ligase 1-like [Cucumis melo var. makuwa][more]
XP_004146311.13.5e-26284.344-coumarate--CoA ligase 1 [Cucumis sativus] >KGN65444.1 hypothetical protein Csa... [more]
KAG6589713.11.8e-25382.294-coumarate--CoA ligase 1, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
M4ISH04.7e-21769.314-coumarate--CoA ligase CCL1 OS=Humulus lupulus OX=3486 GN=CCL1 PE=1 SV=1[more]
O241461.1e-21669.114-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1[more]
P316841.8e-21668.794-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1[more]
O241453.1e-21668.944-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1[more]
P316851.2e-21568.624-coumarate--CoA ligase 2 OS=Solanum tuberosum OX=4113 GN=4CL2 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3CN411.4e-26785.614-coumarate--CoA ligase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3BWP01.4e-26785.614-coumarate--CoA ligase 1-like OS=Cucumis melo OX=3656 GN=LOC103494274 PE=4 SV=1[more]
A0A5A7UT302.3e-26785.314-coumarate--CoA ligase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s... [more]
A0A0A0LWT61.7e-26284.34Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G423020 PE=4 SV=1[more]
A0A6J1E5C54.7e-25281.574-coumarate--CoA ligase 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT1G51680.12.5e-20565.234-coumarate:CoA ligase 1 [more]
AT3G21240.19.5e-20564.994-coumarate:CoA ligase 2 [more]
AT1G51680.31.5e-19465.964-coumarate:CoA ligase 1 [more]
AT1G65060.16.6e-19061.134-coumarate:CoA ligase 3 [more]
AT3G21230.12.3e-18257.604-coumarate:CoA ligase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (PI 537277) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 38..451
e-value: 1.3E-110
score: 369.9
NoneNo IPR availableGENE3D3.30.300.30coord: 447..561
e-value: 4.0E-29
score: 103.3
NoneNo IPR availablePANTHERPTHR24096LONG-CHAIN-FATTY-ACID--COA LIGASEcoord: 7..557
NoneNo IPR availablePANTHERPTHR24096:SF3344-COUMARATE:COA LIGASE-LIKE PROTEINcoord: 7..557
NoneNo IPR availableCDDcd059044CLcoord: 22..554
e-value: 0.0
score: 757.537
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 15..560
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 460..548
e-value: 1.0E-13
score: 52.1
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 10..446
e-value: 6.2E-162
score: 541.2
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 195..206

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CcUC06G123190CcUC06G123190gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CcUC06G123190.1-exonCcUC06G123190.1-exon-CicolChr06:25564221..25564522exon
CcUC06G123190.1-exonCcUC06G123190.1-exon-CicolChr06:25564910..25565051exon
CcUC06G123190.1-exonCcUC06G123190.1-exon-CicolChr06:25565144..25565357exon
CcUC06G123190.1-exonCcUC06G123190.1-exon-CicolChr06:25567171..25567369exon
CcUC06G123190.1-exonCcUC06G123190.1-exon-CicolChr06:25567443..25568597exon
CcUC06G123190.1-exonCcUC06G123190.1-exon-CicolChr06:25570536..25570766exon


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CcUC06G123190.1-three_prime_utrCcUC06G123190.1-three_prime_utr-CicolChr06:25564221..25564402three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CcUC06G123190.1-cdsCcUC06G123190.1-cds-CicolChr06:25564403..25564522CDS
CcUC06G123190.1-cdsCcUC06G123190.1-cds-CicolChr06:25564910..25565051CDS
CcUC06G123190.1-cdsCcUC06G123190.1-cds-CicolChr06:25565144..25565357CDS
CcUC06G123190.1-cdsCcUC06G123190.1-cds-CicolChr06:25567171..25567369CDS
CcUC06G123190.1-cdsCcUC06G123190.1-cds-CicolChr06:25567443..25568462CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CcUC06G123190.1-five_prime_utrCcUC06G123190.1-five_prime_utr-CicolChr06:25568463..25568597five_prime_UTR
CcUC06G123190.1-five_prime_utrCcUC06G123190.1-five_prime_utr-CicolChr06:25570536..25570766five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CcUC06G123190.1CcUC06G123190.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016405 CoA-ligase activity