Carg23125-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg23125-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	ethylene-responsive transcription factor ERF084-like
Location	Carg_Chr08: 3232129 .. 3232707 (-)
Sequence length	579
RNA-Seq Expression	Carg23125-RA
Synteny	Carg23125-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATTTCCGATCAGCCAATTGACTCTCTCTACAGAGCCTTCTTCCAAACCCAATTCTCCCAATCCCCACCGCCGGACTCCCAGCTCGACGGAATCGCCGCCGTCGTCGGCCGGAAAGTCCTATTCGGCGACATTCCTCCCTCAACCAGCGGATCAAAGACGCCGGCCGTTACGGGGCAGAAGAGTTATAGAGGAGTACGAAAGCGGCCGTGGGGACGGTGGTCGGCAGAGATACGAGACCGAATCGGACGGTGCAGACACTGGCTGGGAACGTTCGATACAGCGGAAGAGGCGGCGCGTGCATACGACGCGGCGGCGAGGCGGTTGAGAGGATCCAAGGCGCGAACTAACTTCGAAATGCCGTTGGTTGTGCCGCTTGAGTCCACATCGTCATCATCCACGTCATCAGTGGAAGGGAAAAGGAATGGTAATAATAGTAACAAGTTGAAGGCGGTGAAGAAACCAAATGGACGGAAATGTGCGGTGGTTACGACGGCGGCGCATTTGTTCAGCCGTGGAATGGAGCTTGATTTGAAACTGGGTGTTGACTTTGTTAATGGTGGTCAAAGCGCATCATAA

mRNA sequence

ATGATTTCCGATCAGCCAATTGACTCTCTCTACAGAGCCTTCTTCCAAACCCAATTCTCCCAATCCCCACCGCCGGACTCCCAGCTCGACGGAATCGCCGCCGTCGTCGGCCGGAAAGTCCTATTCGGCGACATTCCTCCCTCAACCAGCGGATCAAAGACGCCGGCCGTTACGGGGCAGAAGAGTTATAGAGGAGTACGAAAGCGGCCGTGGGGACGGTGGTCGGCAGAGATACGAGACCGAATCGGACGGTGCAGACACTGGCTGGGAACGTTCGATACAGCGGAAGAGGCGGCGCGTGCATACGACGCGGCGGCGAGGCGGTTGAGAGGATCCAAGGCGCGAACTAACTTCGAAATGCCGTTGGTTGTGCCGCTTGAGTCCACATCGTCATCATCCACGTCATCAGTGGAAGGGAAAAGGAATGGTAATAATAGTAACAAGTTGAAGGCGGTGAAGAAACCAAATGGACGGAAATGTGCGGTGGTTACGACGGCGGCGCATTTGTTCAGCCGTGGAATGGAGCTTGATTTGAAACTGGGTGTTGACTTTGTTAATGGTGGTCAAAGCGCATCATAA

Coding sequence (CDS)

ATGATTTCCGATCAGCCAATTGACTCTCTCTACAGAGCCTTCTTCCAAACCCAATTCTCCCAATCCCCACCGCCGGACTCCCAGCTCGACGGAATCGCCGCCGTCGTCGGCCGGAAAGTCCTATTCGGCGACATTCCTCCCTCAACCAGCGGATCAAAGACGCCGGCCGTTACGGGGCAGAAGAGTTATAGAGGAGTACGAAAGCGGCCGTGGGGACGGTGGTCGGCAGAGATACGAGACCGAATCGGACGGTGCAGACACTGGCTGGGAACGTTCGATACAGCGGAAGAGGCGGCGCGTGCATACGACGCGGCGGCGAGGCGGTTGAGAGGATCCAAGGCGCGAACTAACTTCGAAATGCCGTTGGTTGTGCCGCTTGAGTCCACATCGTCATCATCCACGTCATCAGTGGAAGGGAAAAGGAATGGTAATAATAGTAACAAGTTGAAGGCGGTGAAGAAACCAAATGGACGGAAATGTGCGGTGGTTACGACGGCGGCGCATTTGTTCAGCCGTGGAATGGAGCTTGATTTGAAACTGGGTGTTGACTTTGTTAATGGTGGTCAAAGCGCATCATAA

Protein sequence

MISDQPIDSLYRAFFQTQFSQSPPPDSQLDGIAAVVGRKVLFGDIPPSTSGSKTPAVTGQKSYRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKARTNFEMPLVVPLESTSSSSTSSVEGKRNGNNSNKLKAVKKPNGRKCAVVTTAAHLFSRGMELDLKLGVDFVNGGQSAS

Homology

[Close]

BLAST of Carg23125-RA vs. NCBI nr
Match: KAG6593374.1 (Ethylene-responsive transcription factor, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025723.1 Ethylene-responsive transcription factor, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 381.7 bits (979), Expect = 3.7e-102
Identity = 192/192 (100.00%), Postives = 192/192 (100.00%), Query Frame = 0


Query: 1   MISDQPIDSLYRAFFQTQFSQSPPPDSQLDGIAAVVGRKVLFGDIPPSTSGSKTPAVTGQ 60
           MISDQPIDSLYRAFFQTQFSQSPPPDSQLDGIAAVVGRKVLFGDIPPSTSGSKTPAVTGQ
Sbjct: 1   MISDQPIDSLYRAFFQTQFSQSPPPDSQLDGIAAVVGRKVLFGDIPPSTSGSKTPAVTGQ 60

Query: 61  KSYRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKARTNFEM 120
           KSYRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKARTNFEM
Sbjct: 61  KSYRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKARTNFEM 120

Query: 121 PLVVPLESTSSSSTSSVEGKRNGNNSNKLKAVKKPNGRKCAVVTTAAHLFSRGMELDLKL 180
           PLVVPLESTSSSSTSSVEGKRNGNNSNKLKAVKKPNGRKCAVVTTAAHLFSRGMELDLKL
Sbjct: 121 PLVVPLESTSSSSTSSVEGKRNGNNSNKLKAVKKPNGRKCAVVTTAAHLFSRGMELDLKL 180

Query: 181 GVDFVNGGQSAS 193
           GVDFVNGGQSAS
Sbjct: 181 GVDFVNGGQSAS 192

[Close]

BLAST of Carg23125-RA vs. NCBI nr
Match: XP_022960429.1 (ethylene-responsive transcription factor ERF084-like [Cucurbita moschata])

HSP 1 Score: 378.3 bits (970), Expect = 4.1e-101
Identity = 190/192 (98.96%), Postives = 190/192 (98.96%), Query Frame = 0


Query: 1   MISDQPIDSLYRAFFQTQFSQSPPPDSQLDGIAAVVGRKVLFGDIPPSTSGSKTPAVTGQ 60
           MISDQPIDSLYRAFFQTQFSQSPPPDSQLDGIAAVVGRKVLFGDIPPSTSGSKTPAVTGQ
Sbjct: 1   MISDQPIDSLYRAFFQTQFSQSPPPDSQLDGIAAVVGRKVLFGDIPPSTSGSKTPAVTGQ 60

Query: 61  KSYRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKARTNFEM 120
           KSYRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKARTNFEM
Sbjct: 61  KSYRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKARTNFEM 120

Query: 121 PLVVPLESTSSSSTSSVEGKRNGNNSNKLKAVKKPNGRKCAVVTTAAHLFSRGMELDLKL 180
           PLVVPLESTSSSSTSSVEGKRNGNN NKLKAVKKPNGRKCAVVTTAAHLFSRGMELDLKL
Sbjct: 121 PLVVPLESTSSSSTSSVEGKRNGNNGNKLKAVKKPNGRKCAVVTTAAHLFSRGMELDLKL 180

Query: 181 GVDFVNGGQSAS 193
           G DFVNGGQSAS
Sbjct: 181 GFDFVNGGQSAS 192

[Close]

BLAST of Carg23125-RA vs. NCBI nr
Match: XP_023004864.1 (ethylene-responsive transcription factor ERF084 [Cucurbita maxima])

HSP 1 Score: 376.3 bits (965), Expect = 1.5e-100
Identity = 189/192 (98.44%), Postives = 190/192 (98.96%), Query Frame = 0


Query: 1   MISDQPIDSLYRAFFQTQFSQSPPPDSQLDGIAAVVGRKVLFGDIPPSTSGSKTPAVTGQ 60
           MISDQPIDSLYRAFFQTQFSQSPPPDSQLDGIAAVVGRKVLFGDIPPSTSGSK PAVTGQ
Sbjct: 1   MISDQPIDSLYRAFFQTQFSQSPPPDSQLDGIAAVVGRKVLFGDIPPSTSGSKAPAVTGQ 60

Query: 61  KSYRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKARTNFEM 120
           KSYRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKARTNFEM
Sbjct: 61  KSYRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKARTNFEM 120

Query: 121 PLVVPLESTSSSSTSSVEGKRNGNNSNKLKAVKKPNGRKCAVVTTAAHLFSRGMELDLKL 180
           PLVVPLESTSSSSTSSVEGKRNGNN NKLKAVKKP+GRKCAVVTTAAHLFSRGMELDLKL
Sbjct: 121 PLVVPLESTSSSSTSSVEGKRNGNNGNKLKAVKKPSGRKCAVVTTAAHLFSRGMELDLKL 180

Query: 181 GVDFVNGGQSAS 193
           GVDFVNGGQSAS
Sbjct: 181 GVDFVNGGQSAS 192

[Close]

BLAST of Carg23125-RA vs. NCBI nr
Match: XP_023514530.1 (ethylene-responsive transcription factor ERF084 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 375.2 bits (962), Expect = 3.4e-100
Identity = 189/192 (98.44%), Postives = 190/192 (98.96%), Query Frame = 0


Query: 1   MISDQPIDSLYRAFFQTQFSQSPPPDSQLDGIAAVVGRKVLFGDIPPSTSGSKTPAVTGQ 60
           MISDQPIDSLYRAFFQTQFSQSPPPDSQLDGIAAVVGRKVLFGDIPPSTS SKTPAVTGQ
Sbjct: 1   MISDQPIDSLYRAFFQTQFSQSPPPDSQLDGIAAVVGRKVLFGDIPPSTSRSKTPAVTGQ 60

Query: 61  KSYRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKARTNFEM 120
           KSYRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKARTNFEM
Sbjct: 61  KSYRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKARTNFEM 120

Query: 121 PLVVPLESTSSSSTSSVEGKRNGNNSNKLKAVKKPNGRKCAVVTTAAHLFSRGMELDLKL 180
           PLVVPLESTSSSSTSSVEGKRNGNN NKLKAVKKP+GRKCAVVTTAAHLFSRGMELDLKL
Sbjct: 121 PLVVPLESTSSSSTSSVEGKRNGNNGNKLKAVKKPSGRKCAVVTTAAHLFSRGMELDLKL 180

Query: 181 GVDFVNGGQSAS 193
           GVDFVNGGQSAS
Sbjct: 181 GVDFVNGGQSAS 192

[Close]

BLAST of Carg23125-RA vs. NCBI nr
Match: KAA0058723.1 (AP2 domain-containing protein [Cucumis melo var. makuwa] >TYK10531.1 AP2 domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 264.2 bits (674), Expect = 8.6e-67
Identity = 141/188 (75.00%), Postives = 154/188 (81.91%), Query Frame = 0


Query: 1   MISDQPIDSLYRAFFQTQFSQSPPPDSQLDGIAAVVGRKVLFGDI---PPSTSGSKTPAV 60
           MI+  PI  LY  FF+TQF  SPPP+SQLDGIAAVVGR+VLFG+     P+ S S T A+
Sbjct: 1   MINTHPIPPLYNTFFETQFPHSPPPESQLDGIAAVVGREVLFGEYNRPTPTKSSSSTSAM 60

Query: 61  ---TGQKSYRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKA 120
              TGQ+SYRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKA
Sbjct: 61  APTTGQRSYRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKA 120

Query: 121 RTNFEMPLVVPLESTSSSSTSSVEGKRNGNNSNKLKAVKKPNGRKCAVVTTAAHLFSRGM 180
           RTNFEMPLVVP+ESTS+ STSSVE KRNGN   K       N RKC+VVT+AAHLFS G+
Sbjct: 121 RTNFEMPLVVPVESTSAWSTSSVEVKRNGNKLKK-------NERKCSVVTSAAHLFSPGV 180

Query: 181 ELDLKLGV 183
           ELDLKLGV
Sbjct: 181 ELDLKLGV 181

[Close]

BLAST of Carg23125-RA vs. ExPASy Swiss-Prot
Match: Q9M8M5 (Ethylene-responsive transcription factor ERF084 OS=Arabidopsis thaliana OX=3702 GN=ERF084 PE=2 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 1.4e-35
Identity = 96/203 (47.29%), Postives = 113/203 (55.67%), Query Frame = 0


Query: 19  FSQSPPPDSQL------DGIAAVVGRKVLFGDIPPSTS----------GSKTPAVTGQKS 78
           FS +P P+ +       D IAAVVG  VLFGD    +           G K P  TG   
Sbjct: 58  FSFNPKPEPENTHRVMDDSIAAVVGENVLFGDKNKVSDHLTKEGGVKRGRKMPQKTG--G 117

Query: 79  YRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKARTNFEMPL 138
           + GVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRG+KA+TNF +P 
Sbjct: 118 FMGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGTKAKTNFVIPP 177

Query: 139 VVPLESTSSSSTSSVEGKRNGNNSNKLKAVKKPNGRKCAVVTTAAHLF------------ 183
           + P E   +   + +  K+      K K  KK + RKC  VT+ A LF            
Sbjct: 178 LFPKEIAQAQEDNRMRQKQ------KKKKKKKVSVRKCVKVTSVAQLFDDANFINSSSIK 237

[Close]

BLAST of Carg23125-RA vs. ExPASy Swiss-Prot
Match: Q9SSA8 (Ethylene-responsive transcription factor RAP2-12 OS=Arabidopsis thaliana OX=3702 GN=RAP2-12 PE=1 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 1.8e-19
Identity = 62/140 (44.29%), Postives = 81/140 (57.86%), Query Frame = 0


Query: 19  FSQSPPPDSQLDGIAAVVGRKV--LFGDIPPSTSGSKTPAVTGQKSYRGVRKRPWGRWSA 78
           F+ +P P        +V G+KV  L GD   S +  +      +  YRG+R+RPWG+W+A
Sbjct: 85  FTSTPKPAVSAAAEGSVFGKKVTGLDGDAEKSANRKR------KNQYRGIRQRPWGKWAA 144

Query: 79  EIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKARTNFEMPLVVPLESTSSSSTSS 138
           EIRD     R WLGTF TAEEAARAYDAAARR+RGSKA+ NF      P E+  ++S   
Sbjct: 145 EIRDPREGARIWLGTFKTAEEAARAYDAAARRIRGSKAKVNF------PEENMKANS--- 204

Query: 139 VEGKRNGNNSNKLKAVKKPN 157
              ++    +N  K V KPN
Sbjct: 205 ---QKRSVKANLQKPVAKPN 206

[Close]

BLAST of Carg23125-RA vs. ExPASy Swiss-Prot
Match: Q9LUM4 (Ethylene-responsive transcription factor RAP2-2 OS=Arabidopsis thaliana OX=3702 GN=RAP2-2 PE=1 SV=2)

HSP 1 Score: 95.1 bits (235), Expect = 9.0e-19
Identity = 56/111 (50.45%), Postives = 69/111 (62.16%), Query Frame = 0


Query: 60  QKSYRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKARTNFE 119
           +  YRG+R+RPWG+W+AEIRD     R WLGTFDTAEEAARAYDAAARR+RG+KA+ NF 
Sbjct: 125 KNQYRGIRQRPWGKWAAEIRDPRKGSREWLGTFDTAEEAARAYDAAARRIRGTKAKVNF- 184

Query: 120 MPLVVPLESTSSSSTSSVEGKRNGNNSNKL-KAVKKPNGRKCAVVTTAAHL 170
                        + S V  KR    +N L K+V KPN +   +V    HL
Sbjct: 185 ---------PEEKNPSVVSQKRPSAKTNNLQKSVAKPN-KSVTLVQQPTHL 224

[Close]

BLAST of Carg23125-RA vs. ExPASy Swiss-Prot
Match: Q9LW49 (Ethylene-responsive transcription factor 4 OS=Nicotiana sylvestris OX=4096 GN=ERF4 PE=2 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 2.0e-18
Identity = 59/108 (54.63%), Postives = 67/108 (62.04%), Query Frame = 0


Query: 36  VGRKVLFGDIPPSTSGSKTPAVTGQKSYRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTA 95
           V  KV  GD+       KT  V  +  YRGVRKRPWGR++AEIRD   + R WLGTFDTA
Sbjct: 3   VKNKVSNGDL--KGGNVKTNGVK-EVHYRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTA 62

Query: 96  EEAARAYDAAARRLRGSKARTNFEMPLVVPLESTSSSSTSSVEGKRNG 144
           EEAA+AYD AAR  RG KA+TNF  PL    +STS SST        G
Sbjct: 63  EEAAKAYDTAAREFRGPKAKTNF--PLPSENQSTSHSSTMESSSGETG 105

[Close]

BLAST of Carg23125-RA vs. ExPASy Swiss-Prot
Match: Q9SAD4 (Ethylene-responsive transcription factor ESR1 OS=Arabidopsis thaliana OX=3702 GN=ESR1 PE=1 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 5.9e-18
Identity = 43/62 (69.35%), Postives = 51/62 (82.26%), Query Frame = 0


Query: 63  YRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKARTNFEMPL 122
           YRGVR+RPWGR++AEIRD + + R WLGTFDTAE+AA AYD+AAR  RG+KARTNF  P 
Sbjct: 57  YRGVRRRPWGRYAAEIRDPMSKERRWLGTFDTAEQAACAYDSAARAFRGAKARTNFTYPT 116

Query: 123 VV 125
            V
Sbjct: 117 AV 118

[Close]

BLAST of Carg23125-RA vs. ExPASy TrEMBL
Match: A0A6J1HB06 (ethylene-responsive transcription factor ERF084-like OS=Cucurbita moschata OX=3662 GN=LOC111461162 PE=4 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 2.0e-101
Identity = 190/192 (98.96%), Postives = 190/192 (98.96%), Query Frame = 0


Query: 1   MISDQPIDSLYRAFFQTQFSQSPPPDSQLDGIAAVVGRKVLFGDIPPSTSGSKTPAVTGQ 60
           MISDQPIDSLYRAFFQTQFSQSPPPDSQLDGIAAVVGRKVLFGDIPPSTSGSKTPAVTGQ
Sbjct: 1   MISDQPIDSLYRAFFQTQFSQSPPPDSQLDGIAAVVGRKVLFGDIPPSTSGSKTPAVTGQ 60

Query: 61  KSYRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKARTNFEM 120
           KSYRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKARTNFEM
Sbjct: 61  KSYRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKARTNFEM 120

Query: 121 PLVVPLESTSSSSTSSVEGKRNGNNSNKLKAVKKPNGRKCAVVTTAAHLFSRGMELDLKL 180
           PLVVPLESTSSSSTSSVEGKRNGNN NKLKAVKKPNGRKCAVVTTAAHLFSRGMELDLKL
Sbjct: 121 PLVVPLESTSSSSTSSVEGKRNGNNGNKLKAVKKPNGRKCAVVTTAAHLFSRGMELDLKL 180

Query: 181 GVDFVNGGQSAS 193
           G DFVNGGQSAS
Sbjct: 181 GFDFVNGGQSAS 192

[Close]

BLAST of Carg23125-RA vs. ExPASy TrEMBL
Match: A0A6J1KXI6 (ethylene-responsive transcription factor ERF084 OS=Cucurbita maxima OX=3661 GN=LOC111498043 PE=4 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 7.5e-101
Identity = 189/192 (98.44%), Postives = 190/192 (98.96%), Query Frame = 0


Query: 1   MISDQPIDSLYRAFFQTQFSQSPPPDSQLDGIAAVVGRKVLFGDIPPSTSGSKTPAVTGQ 60
           MISDQPIDSLYRAFFQTQFSQSPPPDSQLDGIAAVVGRKVLFGDIPPSTSGSK PAVTGQ
Sbjct: 1   MISDQPIDSLYRAFFQTQFSQSPPPDSQLDGIAAVVGRKVLFGDIPPSTSGSKAPAVTGQ 60

Query: 61  KSYRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKARTNFEM 120
           KSYRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKARTNFEM
Sbjct: 61  KSYRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKARTNFEM 120

Query: 121 PLVVPLESTSSSSTSSVEGKRNGNNSNKLKAVKKPNGRKCAVVTTAAHLFSRGMELDLKL 180
           PLVVPLESTSSSSTSSVEGKRNGNN NKLKAVKKP+GRKCAVVTTAAHLFSRGMELDLKL
Sbjct: 121 PLVVPLESTSSSSTSSVEGKRNGNNGNKLKAVKKPSGRKCAVVTTAAHLFSRGMELDLKL 180

Query: 181 GVDFVNGGQSAS 193
           GVDFVNGGQSAS
Sbjct: 181 GVDFVNGGQSAS 192

[Close]

BLAST of Carg23125-RA vs. ExPASy TrEMBL
Match: A0A5A7US44 (AP2 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001820 PE=4 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 4.1e-67
Identity = 141/188 (75.00%), Postives = 154/188 (81.91%), Query Frame = 0


Query: 1   MISDQPIDSLYRAFFQTQFSQSPPPDSQLDGIAAVVGRKVLFGDI---PPSTSGSKTPAV 60
           MI+  PI  LY  FF+TQF  SPPP+SQLDGIAAVVGR+VLFG+     P+ S S T A+
Sbjct: 1   MINTHPIPPLYNTFFETQFPHSPPPESQLDGIAAVVGREVLFGEYNRPTPTKSSSSTSAM 60

Query: 61  ---TGQKSYRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKA 120
              TGQ+SYRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKA
Sbjct: 61  APTTGQRSYRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKA 120

Query: 121 RTNFEMPLVVPLESTSSSSTSSVEGKRNGNNSNKLKAVKKPNGRKCAVVTTAAHLFSRGM 180
           RTNFEMPLVVP+ESTS+ STSSVE KRNGN   K       N RKC+VVT+AAHLFS G+
Sbjct: 121 RTNFEMPLVVPVESTSAWSTSSVEVKRNGNKLKK-------NERKCSVVTSAAHLFSPGV 180

Query: 181 ELDLKLGV 183
           ELDLKLGV
Sbjct: 181 ELDLKLGV 181

[Close]

BLAST of Carg23125-RA vs. ExPASy TrEMBL
Match: A0A0A0KA16 (AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G432130 PE=4 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 5.1e-65
Identity = 140/187 (74.87%), Postives = 149/187 (79.68%), Query Frame = 0


Query: 1   MISDQPIDSLYRAFFQTQFSQSPPPDSQLDGIAAVVGRKVLFGDIPP-----STSGSKTP 60
           MI+  PI  LY  F    F  SPPPDSQLDGIAAVVGR+VLFGD  P     S+S S T 
Sbjct: 1   MINTHPIPPLYNTF----FPHSPPPDSQLDGIAAVVGRQVLFGDNRPTPTKSSSSTSATA 60

Query: 61  AVTGQKSYRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKAR 120
             TGQ+SYRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKAR
Sbjct: 61  PTTGQRSYRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKAR 120

Query: 121 TNFEMPLVVPLESTSSSSTSSVEGKRNGNNSNKLKAVKKPNGRKCAVVTTAAHLFSRGME 180
           TNFEMPLVVP+ESTS+ STSSVE KRNGN   K       N RKC+VVT+AAHLFS G+E
Sbjct: 121 TNFEMPLVVPMESTSAWSTSSVEVKRNGNKLKK-------NDRKCSVVTSAAHLFSPGVE 176

Query: 181 LDLKLGV 183
           LDLKLGV
Sbjct: 181 LDLKLGV 176

[Close]

BLAST of Carg23125-RA vs. ExPASy TrEMBL
Match: A0A6J1DQ41 (ethylene-responsive transcription factor ERF084 OS=Momordica charantia OX=3673 GN=LOC111022048 PE=4 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 3.0e-57
Identity = 133/185 (71.89%), Postives = 145/185 (78.38%), Query Frame = 0


Query: 1   MISDQPIDSLYRAFFQTQFSQSPPPDSQLDGIAAVVGRKVLFG----------DIPPSTS 60
           MI+D  I SLY AFF+TQF   PPP  QLDGIAAVVGR VLFG          D   + S
Sbjct: 14  MINDMAIQSLYEAFFETQFLPEPPP-VQLDGIAAVVGRHVLFGGGGAGAGKNDDASSAAS 73

Query: 61  GSKTPAV-TGQKSYRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRL 120
            SK  AV TGQ+SYRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRL
Sbjct: 74  ASKKAAVATGQRSYRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRL 133

Query: 121 RGSKARTNFEMPLVVPLESTSSSST--SSVEGKRNGN-NSNKLKAVKKPNGRKCAVVTTA 172
           RGSKARTNFE+PLVVPL+STSSSS+  S +    N N N+NKLK V+K NGRKC+VVT+ 
Sbjct: 134 RGSKARTNFEIPLVVPLQSTSSSSSTLSKMNPDINININNNKLKKVQK-NGRKCSVVTSV 193

[Close]

BLAST of Carg23125-RA vs. TAIR 10
Match: AT1G80580.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 151.0 bits (380), Expect = 9.8e-37
Identity = 96/203 (47.29%), Postives = 113/203 (55.67%), Query Frame = 0


Query: 19  FSQSPPPDSQL------DGIAAVVGRKVLFGDIPPSTS----------GSKTPAVTGQKS 78
           FS +P P+ +       D IAAVVG  VLFGD    +           G K P  TG   
Sbjct: 58  FSFNPKPEPENTHRVMDDSIAAVVGENVLFGDKNKVSDHLTKEGGVKRGRKMPQKTG--G 117

Query: 79  YRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKARTNFEMPL 138
           + GVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRG+KA+TNF +P 
Sbjct: 118 FMGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGTKAKTNFVIPP 177

Query: 139 VVPLESTSSSSTSSVEGKRNGNNSNKLKAVKKPNGRKCAVVTTAAHLF------------ 183
           + P E   +   + +  K+      K K  KK + RKC  VT+ A LF            
Sbjct: 178 LFPKEIAQAQEDNRMRQKQ------KKKKKKKVSVRKCVKVTSVAQLFDDANFINSSSIK 237

[Close]

BLAST of Carg23125-RA vs. TAIR 10
Match: AT1G53910.1 (related to AP2 12 )

HSP 1 Score: 97.4 bits (241), Expect = 1.3e-20
Identity = 62/140 (44.29%), Postives = 81/140 (57.86%), Query Frame = 0


Query: 19  FSQSPPPDSQLDGIAAVVGRKV--LFGDIPPSTSGSKTPAVTGQKSYRGVRKRPWGRWSA 78
           F+ +P P        +V G+KV  L GD   S +  +      +  YRG+R+RPWG+W+A
Sbjct: 85  FTSTPKPAVSAAAEGSVFGKKVTGLDGDAEKSANRKR------KNQYRGIRQRPWGKWAA 144

Query: 79  EIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKARTNFEMPLVVPLESTSSSSTSS 138
           EIRD     R WLGTF TAEEAARAYDAAARR+RGSKA+ NF      P E+  ++S   
Sbjct: 145 EIRDPREGARIWLGTFKTAEEAARAYDAAARRIRGSKAKVNF------PEENMKANS--- 204

Query: 139 VEGKRNGNNSNKLKAVKKPN 157
              ++    +N  K V KPN
Sbjct: 205 ---QKRSVKANLQKPVAKPN 206

[Close]

BLAST of Carg23125-RA vs. TAIR 10
Match: AT1G53910.2 (related to AP2 12 )

HSP 1 Score: 97.4 bits (241), Expect = 1.3e-20
Identity = 62/140 (44.29%), Postives = 81/140 (57.86%), Query Frame = 0


Query: 19  FSQSPPPDSQLDGIAAVVGRKV--LFGDIPPSTSGSKTPAVTGQKSYRGVRKRPWGRWSA 78
           F+ +P P        +V G+KV  L GD   S +  +      +  YRG+R+RPWG+W+A
Sbjct: 85  FTSTPKPAVSAAAEGSVFGKKVTGLDGDAEKSANRKR------KNQYRGIRQRPWGKWAA 144

Query: 79  EIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKARTNFEMPLVVPLESTSSSSTSS 138
           EIRD     R WLGTF TAEEAARAYDAAARR+RGSKA+ NF      P E+  ++S   
Sbjct: 145 EIRDPREGARIWLGTFKTAEEAARAYDAAARRIRGSKAKVNF------PEENMKANS--- 204

Query: 139 VEGKRNGNNSNKLKAVKKPN 157
              ++    +N  K V KPN
Sbjct: 205 ---QKRSVKANLQKPVAKPN 206

[Close]

BLAST of Carg23125-RA vs. TAIR 10
Match: AT1G53910.3 (related to AP2 12 )

HSP 1 Score: 96.7 bits (239), Expect = 2.2e-20
Identity = 63/140 (45.00%), Postives = 82/140 (58.57%), Query Frame = 0


Query: 19  FSQSPPPDSQLDGIAAVVGRKV--LFGDIPPSTSGSKTPAVTGQKSYRGVRKRPWGRWSA 78
           F+ +P P   +   A V G+KV  L GD   S +  +      +  YRG+R+RPWG+W+A
Sbjct: 85  FTSTPKP--AVSAAAEVFGKKVTGLDGDAEKSANRKR------KNQYRGIRQRPWGKWAA 144

Query: 79  EIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKARTNFEMPLVVPLESTSSSSTSS 138
           EIRD     R WLGTF TAEEAARAYDAAARR+RGSKA+ NF      P E+  ++S   
Sbjct: 145 EIRDPREGARIWLGTFKTAEEAARAYDAAARRIRGSKAKVNF------PEENMKANS--- 204

Query: 139 VEGKRNGNNSNKLKAVKKPN 157
              ++    +N  K V KPN
Sbjct: 205 ---QKRSVKANLQKPVAKPN 204

[Close]

BLAST of Carg23125-RA vs. TAIR 10
Match: AT3G14230.1 (related to AP2 2 )

HSP 1 Score: 95.1 bits (235), Expect = 6.4e-20
Identity = 56/111 (50.45%), Postives = 69/111 (62.16%), Query Frame = 0


Query: 60  QKSYRGVRKRPWGRWSAEIRDRIGRCRHWLGTFDTAEEAARAYDAAARRLRGSKARTNFE 119
           +  YRG+R+RPWG+W+AEIRD     R WLGTFDTAEEAARAYDAAARR+RG+KA+ NF 
Sbjct: 125 KNQYRGIRQRPWGKWAAEIRDPRKGSREWLGTFDTAEEAARAYDAAARRIRGTKAKVNF- 184

Query: 120 MPLVVPLESTSSSSTSSVEGKRNGNNSNKL-KAVKKPNGRKCAVVTTAAHL 170
                        + S V  KR    +N L K+V KPN +   +V    HL
Sbjct: 185 ---------PEEKNPSVVSQKRPSAKTNNLQKSVAKPN-KSVTLVQQPTHL 224

The following BLAST results are available for this feature:

BLAST of Carg23125-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6593374.1	3.7e-102	100.00	Ethylene-responsive transcription factor, partial [Cucurbita argyrosperma subsp....	[more]
XP_022960429.1	4.1e-101	98.96	ethylene-responsive transcription factor ERF084-like [Cucurbita moschata]	[more]
XP_023004864.1	1.5e-100	98.44	ethylene-responsive transcription factor ERF084 [Cucurbita maxima]	[more]
XP_023514530.1	3.4e-100	98.44	ethylene-responsive transcription factor ERF084 [Cucurbita pepo subsp. pepo]	[more]
KAA0058723.1	8.6e-67	75.00	AP2 domain-containing protein [Cucumis melo var. makuwa] >TYK10531.1 AP2 domain-...	[more]

BLAST of Carg23125-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M8M5	1.4e-35	47.29	Ethylene-responsive transcription factor ERF084 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9SSA8	1.8e-19	44.29	Ethylene-responsive transcription factor RAP2-12 OS=Arabidopsis thaliana OX=3702...	[more]
Q9LUM4	9.0e-19	50.45	Ethylene-responsive transcription factor RAP2-2 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9LW49	2.0e-18	54.63	Ethylene-responsive transcription factor 4 OS=Nicotiana sylvestris OX=4096 GN=ER...	[more]
Q9SAD4	5.9e-18	69.35	Ethylene-responsive transcription factor ESR1 OS=Arabidopsis thaliana OX=3702 GN...	[more]

BLAST of Carg23125-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HB06	2.0e-101	98.96	ethylene-responsive transcription factor ERF084-like OS=Cucurbita moschata OX=36...	[more]
A0A6J1KXI6	7.5e-101	98.44	ethylene-responsive transcription factor ERF084 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A5A7US44	4.1e-67	75.00	AP2 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A0A0KA16	5.1e-65	74.87	AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G432130 PE=...	[more]
A0A6J1DQ41	3.0e-57	71.89	ethylene-responsive transcription factor ERF084 OS=Momordica charantia OX=3673 G...	[more]

BLAST of Carg23125-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G80580.1	9.8e-37	47.29	Integrase-type DNA-binding superfamily protein	[more]
AT1G53910.1	1.3e-20	44.29	related to AP2 12	[more]
AT1G53910.2	1.3e-20	44.29	related to AP2 12	[more]
AT1G53910.3	2.2e-20	45.00	related to AP2 12	[more]
AT3G14230.1	6.4e-20	50.45	related to AP2 2	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001471	AP2/ERF domain	PRINTS	PR00367	ETHRSPELEMNT	coord: 85..101 score: 71.23 coord: 63..74 score: 70.15
IPR001471	AP2/ERF domain	SMART	SM00380	rav1_2	coord: 62..125 e-value: 6.3E-33 score: 125.4
IPR001471	AP2/ERF domain	PFAM	PF00847	AP2	coord: 62..111 e-value: 1.2E-15 score: 57.5
IPR001471	AP2/ERF domain	PROSITE	PS51032	AP2_ERF	coord: 62..119 score: 22.866192
IPR001471	AP2/ERF domain	CDD	cd00018	AP2	coord: 61..119 e-value: 5.47826E-16 score: 66.9115
IPR036955	AP2/ERF domain superfamily	GENE3D	3.30.730.10	AP2/ERF domain	coord: 61..120 e-value: 1.1E-26 score: 94.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 127..153
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 127..151
None	No IPR available	PANTHER	PTHR31677:SF75	ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF084	coord: 51..175
None	No IPR available	PANTHER	PTHR31677	AP2 DOMAIN CLASS TRANSCRIPTION FACTOR	coord: 51..175
IPR016177	DNA-binding domain superfamily	SUPERFAMILY	54171	DNA-binding domain	coord: 62..120

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg23125	Carg23125	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg23125-RA:1	Carg23125-RA:1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg23125-RA:cds	Carg23125-RA:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg23125-RA	Carg23125-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003700	DNA-binding transcription factor activity