Carg21658-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg21658-RA vs. NCBI nr
Match: KAG7028315.1 (Chloride channel protein CLC-f, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1054.3 bits (2725), Expect = 3.6e-304
Identity = 548/548 (100.00%), Postives = 548/548 (100.00%), Query Frame = 0

Query: 1   MAEAEFSDQNRLLRSMEDHKGEDHDLESPDGNGIQPLRNNSGKRGVILDMFHHLNRGGSF 60
           MAEAEFSDQNRLLRSMEDHKGEDHDLESPDGNGIQPLRNNSGKRGVILDMFHHLNRGGSF
Sbjct: 1   MAEAEFSDQNRLLRSMEDHKGEDHDLESPDGNGIQPLRNNSGKRGVILDMFHHLNRGGSF 60

Query: 61  SGRRLSYKRHDMDNHAATFSPTSLNIVGRARTASSSSSSSSDRHNNFNSYPLHAPTGSDT 120
           SGRRLSYKRHDMDNHAATFSPTSLNIVGRARTASSSSSSSSDRHNNFNSYPLHAPTGSDT
Sbjct: 61  SGRRLSYKRHDMDNHAATFSPTSLNIVGRARTASSSSSSSSDRHNNFNSYPLHAPTGSDT 120

Query: 121 EIDENVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRLQ 180
           EIDENVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRLQ
Sbjct: 121 EIDENVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRLQ 180

Query: 181 RMADTWHRILLIPVTGGVIVGMMHGLLEILGQIKQSSTSQRQGFDLLSGVFPTVKAIQAA 240
           RMADTWHRILLIPVTGGVIVGMMHGLLEILGQIKQSSTSQRQGFDLLSGVFPTVKAIQAA
Sbjct: 181 RMADTWHRILLIPVTGGVIVGMMHGLLEILGQIKQSSTSQRQGFDLLSGVFPTVKAIQAA 240

Query: 241 ITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAG 300
           ITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAG
Sbjct: 241 ITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAG 300

Query: 301 SFFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAELP 360
           SFFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAELP
Sbjct: 301 SFFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAELP 360

Query: 361 LYLILGMLCGVVSVAVTRLVAWFSKLFDFIKERFGFPPVVCPALGGLGAGIIALKYPGIL 420
           LYLILGMLCGVVSVAVTRLVAWFSKLFDFIKERFGFPPVVCPALGGLGAGIIALKYPGIL
Sbjct: 361 LYLILGMLCGVVSVAVTRLVAWFSKLFDFIKERFGFPPVVCPALGGLGAGIIALKYPGIL 420

Query: 421 YWGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVG 480
           YWGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVG
Sbjct: 421 YWGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVG 480

Query: 481 AVFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRIL 540
           AVFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRIL
Sbjct: 481 AVFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRIL 540

Query: 541 LPLMASIP 549
           LPLMASIP
Sbjct: 541 LPLMASIP 548

BLAST of Carg21658-RA vs. NCBI nr
Match: KAG6596788.1 (Chloride channel protein CLC-f, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1045.0 bits (2701), Expect = 2.2e-301
Identity = 545/548 (99.45%), Postives = 547/548 (99.82%), Query Frame = 0

Query: 1   MAEAEFSDQNRLLRSMEDHKGEDHDLESPDGNGIQPLRNNSGKRGVILDMFHHLNRGGSF 60
           MAEAEFSDQNRLLRSMEDHKGEDHDLESPDGNGIQPLRNNSGKRGVILDMFHHLNRGGSF
Sbjct: 1   MAEAEFSDQNRLLRSMEDHKGEDHDLESPDGNGIQPLRNNSGKRGVILDMFHHLNRGGSF 60

Query: 61  SGRRLSYKRHDMDNHAATFSPTSLNIVGRARTA-SSSSSSSSDRHNNFNSYPLHAPTGSD 120
           SGRRLSYKRHDMDNHAATFSPTSLNIVGRARTA SSSSSSSSDRHNNFNSYPLHAPTGSD
Sbjct: 61  SGRRLSYKRHDMDNHAATFSPTSLNIVGRARTASSSSSSSSSDRHNNFNSYPLHAPTGSD 120

Query: 121 TEIDENVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRL 180
           TEIDENVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRL
Sbjct: 121 TEIDENVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRL 180

Query: 181 QRMADTWHRILLIPVTGGVIVGMMHGLLEILGQIKQSSTSQRQGFDLLSGVFPTVKAIQA 240
           QRMADTWHRILLIPVTGGVIVGMMHGLLEILGQIKQSSTSQRQGFDLLSGVFPTVKAIQA
Sbjct: 181 QRMADTWHRILLIPVTGGVIVGMMHGLLEILGQIKQSSTSQRQGFDLLSGVFPTVKAIQA 240

Query: 241 AITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVA 300
           AITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVA
Sbjct: 241 AITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVA 300

Query: 301 GSFFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAEL 360
           GSFFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAEL
Sbjct: 301 GSFFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAEL 360

Query: 361 PLYLILGMLCGVVSVAVTRLVAWFSKLFDFIKERFGFPPVVCPALGGLGAGIIALKYPGI 420
           PLYLILGMLCGVVSVAVTRLVAWFSKLFDFIKERFGFPPVVCPALGGLGAGIIALKYPGI
Sbjct: 361 PLYLILGMLCGVVSVAVTRLVAWFSKLFDFIKERFGFPPVVCPALGGLGAGIIALKYPGI 420

Query: 421 LYWGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAV 480
           LYWGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAV
Sbjct: 421 LYWGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAV 480

Query: 481 GAVFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRI 540
           GAVFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRI
Sbjct: 481 GAVFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRI 540

Query: 541 LLPLMASI 548
           LLPLMA++
Sbjct: 541 LLPLMATV 548

BLAST of Carg21658-RA vs. NCBI nr
Match: XP_022949742.1 (chloride channel protein CLC-f-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 1040.4 bits (2689), Expect = 5.4e-300
Identity = 543/548 (99.09%), Postives = 545/548 (99.45%), Query Frame = 0

Query: 1   MAEAEFSDQNRLLRSMEDHKGEDHDLESPDGNGIQPLRNNSGKRGVILDMFHHLNRGGSF 60
           MAEAEFSDQNRLLRSMEDHKGEDHDLES DGNGIQPLRNNSGKRGVILDMFHHLNRGGSF
Sbjct: 1   MAEAEFSDQNRLLRSMEDHKGEDHDLESQDGNGIQPLRNNSGKRGVILDMFHHLNRGGSF 60

Query: 61  SGRRLSYKRHDMDNHAATFSPTSLNIVGRARTA-SSSSSSSSDRHNNFNSYPLHAPTGSD 120
           SGRRLSYKRHDMDNHAATFSPTSLNIVGRARTA SSSSSSSSDRHNNFNSYPLHAPTGSD
Sbjct: 61  SGRRLSYKRHDMDNHAATFSPTSLNIVGRARTASSSSSSSSSDRHNNFNSYPLHAPTGSD 120

Query: 121 TEIDENVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRL 180
           TEIDENVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRL
Sbjct: 121 TEIDENVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRL 180

Query: 181 QRMADTWHRILLIPVTGGVIVGMMHGLLEILGQIKQSSTSQRQGFDLLSGVFPTVKAIQA 240
           QRMADTWHRILLIPVTGGVIVGMMHGLLEILGQIKQSSTSQRQGFDLLSGVFPTVKAIQA
Sbjct: 181 QRMADTWHRILLIPVTGGVIVGMMHGLLEILGQIKQSSTSQRQGFDLLSGVFPTVKAIQA 240

Query: 241 AITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVA 300
           AITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVA
Sbjct: 241 AITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVA 300

Query: 301 GSFFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAEL 360
           GSFFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAEL
Sbjct: 301 GSFFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAEL 360

Query: 361 PLYLILGMLCGVVSVAVTRLVAWFSKLFDFIKERFGFPPVVCPALGGLGAGIIALKYPGI 420
           PLYLILGMLCGVVSVAVTRLVAWFSKLFDFIKERFGFPPVVCPALGGLGAGIIALKYPGI
Sbjct: 361 PLYLILGMLCGVVSVAVTRLVAWFSKLFDFIKERFGFPPVVCPALGGLGAGIIALKYPGI 420

Query: 421 LYWGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAV 480
           LYWGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAV
Sbjct: 421 LYWGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAV 480

Query: 481 GAVFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRI 540
           GAVFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRI
Sbjct: 481 GAVFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRI 540

Query: 541 LLPLMASI 548
           LLPLM ++
Sbjct: 541 LLPLMGAV 548

BLAST of Carg21658-RA vs. NCBI nr
Match: XP_022949733.1 (chloride channel protein CLC-f-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1040.4 bits (2689), Expect = 5.4e-300
Identity = 543/548 (99.09%), Postives = 545/548 (99.45%), Query Frame = 0

Query: 1   MAEAEFSDQNRLLRSMEDHKGEDHDLESPDGNGIQPLRNNSGKRGVILDMFHHLNRGGSF 60
           MAEAEFSDQNRLLRSMEDHKGEDHDLES DGNGIQPLRNNSGKRGVILDMFHHLNRGGSF
Sbjct: 1   MAEAEFSDQNRLLRSMEDHKGEDHDLESQDGNGIQPLRNNSGKRGVILDMFHHLNRGGSF 60

Query: 61  SGRRLSYKRHDMDNHAATFSPTSLNIVGRARTA-SSSSSSSSDRHNNFNSYPLHAPTGSD 120
           SGRRLSYKRHDMDNHAATFSPTSLNIVGRARTA SSSSSSSSDRHNNFNSYPLHAPTGSD
Sbjct: 61  SGRRLSYKRHDMDNHAATFSPTSLNIVGRARTASSSSSSSSSDRHNNFNSYPLHAPTGSD 120

Query: 121 TEIDENVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRL 180
           TEIDENVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRL
Sbjct: 121 TEIDENVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRL 180

Query: 181 QRMADTWHRILLIPVTGGVIVGMMHGLLEILGQIKQSSTSQRQGFDLLSGVFPTVKAIQA 240
           QRMADTWHRILLIPVTGGVIVGMMHGLLEILGQIKQSSTSQRQGFDLLSGVFPTVKAIQA
Sbjct: 181 QRMADTWHRILLIPVTGGVIVGMMHGLLEILGQIKQSSTSQRQGFDLLSGVFPTVKAIQA 240

Query: 241 AITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVA 300
           AITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVA
Sbjct: 241 AITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVA 300

Query: 301 GSFFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAEL 360
           GSFFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAEL
Sbjct: 301 GSFFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAEL 360

Query: 361 PLYLILGMLCGVVSVAVTRLVAWFSKLFDFIKERFGFPPVVCPALGGLGAGIIALKYPGI 420
           PLYLILGMLCGVVSVAVTRLVAWFSKLFDFIKERFGFPPVVCPALGGLGAGIIALKYPGI
Sbjct: 361 PLYLILGMLCGVVSVAVTRLVAWFSKLFDFIKERFGFPPVVCPALGGLGAGIIALKYPGI 420

Query: 421 LYWGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAV 480
           LYWGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAV
Sbjct: 421 LYWGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAV 480

Query: 481 GAVFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRI 540
           GAVFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRI
Sbjct: 481 GAVFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRI 540

Query: 541 LLPLMASI 548
           LLPLM ++
Sbjct: 541 LLPLMGAV 548

BLAST of Carg21658-RA vs. NCBI nr
Match: XP_022949722.1 (chloride channel protein CLC-f-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1040.4 bits (2689), Expect = 5.4e-300
Identity = 543/548 (99.09%), Postives = 545/548 (99.45%), Query Frame = 0

Query: 1   MAEAEFSDQNRLLRSMEDHKGEDHDLESPDGNGIQPLRNNSGKRGVILDMFHHLNRGGSF 60
           MAEAEFSDQNRLLRSMEDHKGEDHDLES DGNGIQPLRNNSGKRGVILDMFHHLNRGGSF
Sbjct: 1   MAEAEFSDQNRLLRSMEDHKGEDHDLESQDGNGIQPLRNNSGKRGVILDMFHHLNRGGSF 60

Query: 61  SGRRLSYKRHDMDNHAATFSPTSLNIVGRARTA-SSSSSSSSDRHNNFNSYPLHAPTGSD 120
           SGRRLSYKRHDMDNHAATFSPTSLNIVGRARTA SSSSSSSSDRHNNFNSYPLHAPTGSD
Sbjct: 61  SGRRLSYKRHDMDNHAATFSPTSLNIVGRARTASSSSSSSSSDRHNNFNSYPLHAPTGSD 120

Query: 121 TEIDENVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRL 180
           TEIDENVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRL
Sbjct: 121 TEIDENVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRL 180

Query: 181 QRMADTWHRILLIPVTGGVIVGMMHGLLEILGQIKQSSTSQRQGFDLLSGVFPTVKAIQA 240
           QRMADTWHRILLIPVTGGVIVGMMHGLLEILGQIKQSSTSQRQGFDLLSGVFPTVKAIQA
Sbjct: 181 QRMADTWHRILLIPVTGGVIVGMMHGLLEILGQIKQSSTSQRQGFDLLSGVFPTVKAIQA 240

Query: 241 AITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVA 300
           AITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVA
Sbjct: 241 AITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVA 300

Query: 301 GSFFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAEL 360
           GSFFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAEL
Sbjct: 301 GSFFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAEL 360

Query: 361 PLYLILGMLCGVVSVAVTRLVAWFSKLFDFIKERFGFPPVVCPALGGLGAGIIALKYPGI 420
           PLYLILGMLCGVVSVAVTRLVAWFSKLFDFIKERFGFPPVVCPALGGLGAGIIALKYPGI
Sbjct: 361 PLYLILGMLCGVVSVAVTRLVAWFSKLFDFIKERFGFPPVVCPALGGLGAGIIALKYPGI 420

Query: 421 LYWGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAV 480
           LYWGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAV
Sbjct: 421 LYWGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAV 480

Query: 481 GAVFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRI 540
           GAVFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRI
Sbjct: 481 GAVFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRI 540

Query: 541 LLPLMASI 548
           LLPLM ++
Sbjct: 541 LLPLMGAV 548

BLAST of Carg21658-RA vs. ExPASy Swiss-Prot
Match: Q8RXR2 (Chloride channel protein CLC-f OS=Arabidopsis thaliana OX=3702 GN=CLC-F PE=2 SV=2)

HSP 1 Score: 724.5 bits (1869), Expect = 8.7e-208
Identity = 400/552 (72.46%), Postives = 449/552 (81.34%), Query Frame = 0

Query: 5   EFSDQNRLLRSMEDHK-----GE-DHDLESPDGNGIQPLRNNSGKRGVILDMFHHLNRGG 64
           E+++   LLRS +  +     GE D D+ES      Q     SG  GV  D+F H++R  
Sbjct: 8   EYNEDRHLLRSTDGDEVGIGGGEGDLDVES------QSPAIRSGAGGV-RDLFKHIDRRF 67

Query: 65  SFSGRRLSYKRHDMDNHAATFSPTSLNIVGRARTASSSSSSSSDRHNNFNSYPLHAPTGS 124
           S SGRRLS+KR  M+N              R   +SSS+ S++   +      LH+    
Sbjct: 68  SLSGRRLSFKR--MENIRVDRE--------RHNPSSSSAFSAAGEEDGGGISNLHSVDDR 127

Query: 125 DTEI---DENVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAA 184
           + E    +E + D+A PEWALLLI CL+G+A G+CVA FN GVHVIHEWAWAGTPNEGAA
Sbjct: 128 NDEYGFDEEVLGDSAPPEWALLLIGCLIGVAAGICVAGFNKGVHVIHEWAWAGTPNEGAA 187

Query: 185 WLRLQRMADTWHRILLIPVTGGVIVGMMHGLLEILGQIKQSSTSQRQGFDLLSGVFPTVK 244
           WLRLQR+ADTWHRILLIPVTGGVIVGMMHGLLEIL QI+QS++SQRQG D L+G++P +K
Sbjct: 188 WLRLQRLADTWHRILLIPVTGGVIVGMMHGLLEILDQIRQSNSSQRQGLDFLAGIYPVIK 247

Query: 245 AIQAAITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFN 304
           AIQAA+TLGTGCSLGPEGPSVDIGKSCANGF LMMENN E+ +IA  AAGAA+GIASGFN
Sbjct: 248 AIQAAVTLGTGCSLGPEGPSVDIGKSCANGFALMMENNRER-RIALTAAGAASGIASGFN 307

Query: 305 AAVAGSFFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKS 364
           AAVAG FFAIETVLRPLRAENSPPFTTAMIIL+SVISSTVS  LLGTQSAFTVP+YDLKS
Sbjct: 308 AAVAGCFFAIETVLRPLRAENSPPFTTAMIILASVISSTVSNALLGTQSAFTVPSYDLKS 367

Query: 365 AAELPLYLILGMLCGVVSVAVTRLVAWFSKLFDFIKERFGFPPVVCPALGGLGAGIIALK 424
           AAELPLYLILGMLCG VSV  +RLV WF+K FDFIK++FG P +VCPALGGLGAGIIALK
Sbjct: 368 AAELPLYLILGMLCGAVSVVFSRLVTWFTKSFDFIKDKFGLPAIVCPALGGLGAGIIALK 427

Query: 425 YPGILYWGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMI 484
           YPGILYWGFTNVEEILHTG   SAPG+WLL Q+AAAKVVATALCKGSGLVGGLYAPSLMI
Sbjct: 428 YPGILYWGFTNVEEILHTGKSASAPGIWLLAQLAAAKVVATALCKGSGLVGGLYAPSLMI 487

Query: 485 GAAVGAVFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTK 544
           GAAVGAVFGGSA E+IN AIPGNAAVAQPQAYALVGMAATLAS+CSVPLTSVLLLFELTK
Sbjct: 488 GAAVGAVFGGSAAEIINRAIPGNAAVAQPQAYALVGMAATLASMCSVPLTSVLLLFELTK 541

Query: 545 DYRILLPLMASI 548
           DYRILLPLM ++
Sbjct: 548 DYRILLPLMGAV 541

BLAST of Carg21658-RA vs. ExPASy Swiss-Prot
Match: Q8GX93 (Chloride channel protein CLC-e OS=Arabidopsis thaliana OX=3702 GN=CLC-E PE=2 SV=2)

HSP 1 Score: 353.6 bits (906), Expect = 4.0e-96
Identity = 210/451 (46.56%), Postives = 285/451 (63.19%), Query Frame = 0

Query: 110 YPLHAPTGSDTEIDENVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGT 169
           +P       +T+ DE   D    +   +  ACL+G+ TG+ V  FN  VH++ +++W G 
Sbjct: 50  FPRLPAAKQETDQDEVGFDQQPSQELAIASACLVGVLTGVSVVLFNNCVHLLRDFSWDGI 109

Query: 170 PNEGAAWLRLQRMADTWHRILLIPVTGGVIVGMMHGLLEILGQIKQSSTSQRQGFDLLSG 229
           P+ GA+WLR   +   W R++L+P  GG++V +++ L E  G   +S+       D +  
Sbjct: 110 PDRGASWLREAPIGSNWLRVILVPTIGGLVVSILNQLRESAG---KSTGDSHSSLDRVKA 169

Query: 230 VF-PTVKAIQAAITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAA 289
           V  P +K + A +TLGTG SLGPEGPSV+IG S A G   +  N S +   + +AAG+AA
Sbjct: 170 VLRPFLKTVAACVTLGTGNSLGPEGPSVEIGASIAKGVNSLF-NKSPQTGFSLLAAGSAA 229

Query: 290 GIASGFNAAVAGSFFAIETVLRPLRAENSP---PFTTAMIILSSVISSTVSTVLLGTQSA 349
           GI+SGFNAAVAG FFA+E+VL P  + +S    P TT+M+ILS+V +S VS + LG++ A
Sbjct: 230 GISSGFNAAVAGCFFAVESVLWPSSSTDSSTSLPNTTSMVILSAVTASVVSEIGLGSEPA 289

Query: 350 FTVPAYDLKSAAELPLYLILGMLCGVVSVAVTRLVAWFSKLFDFIKERFGFPPVVCPALG 409
           F VP YD +S  ELPLYL+LG LCG+VS+A++R  +  +   D + +  G P  V P +G
Sbjct: 290 FKVPDYDFRSPGELPLYLLLGALCGLVSLALSRCTSSMTSAVDSLNKDAGIPKAVFPVMG 349

Query: 410 GLGAGIIALKYPGILYWGFTNVEEILHTGNRPSAPGVW--LLTQIAAAKVVATALCKGSG 469
           GL  GIIAL YP +LYWGF NV+ +L    RP   G+   LL Q+ A K+ ATA C+ SG
Sbjct: 350 GLSVGIIALVYPEVLYWGFQNVDILLE--KRPFVKGLSADLLLQLVAVKIAATAWCRASG 409

Query: 470 LVGGLYAPSLMIGAAVGAVFGGSAVEVINAAIPGN-------AAVAQPQAYALVGMAATL 529
           LVGG YAPSL IG A G  +G    + I  A+  N         VA PQAY LVGMAATL
Sbjct: 410 LVGGYYAPSLFIGGAAGMAYG----KFIGLALAQNPDFNLSILEVASPQAYGLVGMAATL 469

Query: 530 ASVCSVPLTSVLLLFELTKDYRILLPLMASI 548
           A VC VPLT+VLLLFELT+DYRI+LPL+ ++
Sbjct: 470 AGVCQVPLTAVLLLFELTQDYRIVLPLLGAV 490

BLAST of Carg21658-RA vs. ExPASy Swiss-Prot
Match: Q8XTT4 (Putative chloride channel protein ClcB-like OS=Ralstonia solanacearum (strain GMI1000) OX=267608 GN=RSp0020 PE=3 SV=2)

HSP 1 Score: 146.4 bits (368), Expect = 9.7e-34
Identity = 132/417 (31.65%), Postives = 208/417 (49.88%), Query Frame = 0

Query: 136 LLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRLQRMADTWHRILLIPVT 195
           +L IA L+G A  L   AF   +  + +W  AG      A  R    A  W   LL+P  
Sbjct: 1   MLAIAGLIGCAGALATIAFRECLRQL-QWWLAGADQGLVATAR----ALPWWARLLVPTA 60

Query: 196 GGVIVG--MMHGLLEILGQ-IKQSSTSQRQGFDLLSGVFPTVKAIQAAITLGTGCSLGPE 255
           GG++ G  + +GL  I  +  +    +   G  +LS     V++  +  ++ +G S+G E
Sbjct: 61  GGLLAGLTLQYGLKWIPRKGAEDYMEAIAVGDGVLSARQSLVRSASSLCSVASGASIGRE 120

Query: 256 GPSVDIGKSCAN--GFYL--MMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSFFAIETV 315
           GP V +   C +  G  L   M  + E +++  VA GAAAGI S +NA +AG+ F  E V
Sbjct: 121 GPMVQLAAMCGSLLGRVLRHAMPVSVEHMRL-LVACGAAAGITSAYNAPIAGAVFVCEIV 180

Query: 316 LRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAELPLYLILGML 375
              +      P     +++S+V +  +     G  + + +P +D  S  E+  YL LG+ 
Sbjct: 181 FGAITTATLGP-----LLVSAVTADIIVRQFFGYGAVYAMPHFDFVSGWEVLTYLGLGLA 240

Query: 376 CGVVSVAVTRLVAWFSKLFDFIKERFGFPPVVCPALGGLGAGIIALKYPGILYWGFTNVE 435
            G+    +  L+      F     R   P  +  ALGGL  G ++++ P +   G++ V 
Sbjct: 241 AGMAGPLLLGLIDRARGAF----ARTRLPQALRLALGGLIVGALSIRVPEVWGNGYSVVN 300

Query: 436 EILHTGNRPSAPGVW-LLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAVFGGSA 495
             LH      AP +W  +  +   KV ATA   GSG VGG++ P+L  GAA+G ++G   
Sbjct: 301 GFLH------APWLWQTVALVLVCKVGATAASAGSGAVGGVFTPTLFCGAALGLLYGTG- 360

Query: 496 VEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILLPLM 545
              ++A +PG A V  P +YA+VGM A LA+    PL S+L++FE+T  Y+++LPLM
Sbjct: 361 ---MHALLPGAAPV--PVSYAVVGMGALLAATTHAPLMSILMIFEMTLSYQVVLPLM 390

BLAST of Carg21658-RA vs. ExPASy Swiss-Prot
Match: A5F0D5 (H(+)/Cl(-) exchange transporter ClcA OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395) OX=345073 GN=clcA PE=3 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 2.4e-24
Identity = 112/418 (26.79%), Postives = 188/418 (44.98%), Query Frame = 0

Query: 134 WALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRLQRMADTWHRILLIP 193
           +++L ++ L+G+  GL    F   VH++ E       +E  ++L L      W    LI 
Sbjct: 31  FSVLFLSLLVGILAGLVGTYFEQAVHLVSETRTDWLKSEIGSFLPL------WLAAFLIS 90

Query: 194 VTGGVIVGMMHGLLEILGQIKQSSTSQRQGFDLLSGVFPT-------VKAIQAAITLGTG 253
                I   +  +     +   S   + +G   + G+ P        VK       LG+G
Sbjct: 91  AFLAFIGYFL--VHRFAPEAAGSGIPEIEG--AMDGMRPVRWWRVLPVKFFGGMGALGSG 150

Query: 254 CSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSFFAIE 313
             LG EGP+V +G +       +    +E  + + +AAGAA G+A+ FNA +AG  F IE
Sbjct: 151 MVLGREGPTVQMGGAVGRMISDIFRVKNEDTRHSLLAAGAAGGLAAAFNAPLAGIMFVIE 210

Query: 314 TVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAELPLYLILG 373
            +    R       +   +I+S+V ++ V  V+ G  +  T+P YD    + L L+L+LG
Sbjct: 211 EMRPQFR---YTLISVRAVIISAVAANIVFRVINGQDAVITMPQYDAPELSTLGLFLLLG 270

Query: 374 MLCGVVSVAVTRLVAWFSKLFDFIKERFGFPPVVCPALGGLGAGIIALKYPGILYWGFTN 433
            L GV  V    L+     LF           ++  ++ G   G++ L  P +   G + 
Sbjct: 271 ALFGVFGVLFNYLITLAQDLFVKFHRNDRKRYLLTGSMIGGCFGLLLLYVPELTGGGISL 330

Query: 434 VEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAVFGGS 493
           +  I + G      G  +L  +   ++  T LC GSG  GG++AP L +G   G  FG  
Sbjct: 331 IPTITNGGY-----GAGILLLLFVGRIFTTLLCFGSGAPGGIFAPMLALGTLFGYAFG-- 390

Query: 494 AVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILLPLM 545
               + A +       +P  +A+ GM A  A+    P+T +LL+ E+T +Y ++LPL+
Sbjct: 391 ----LIAKVWFPELNIEPGMFAIAGMGALFAATVRAPITGILLVIEMTNNYHLILPLI 424

BLAST of Carg21658-RA vs. ExPASy Swiss-Prot
Match: Q9KM62 (H(+)/Cl(-) exchange transporter ClcA OS=Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) OX=243277 GN=clcA PE=3 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 4.1e-24
Identity = 112/418 (26.79%), Postives = 188/418 (44.98%), Query Frame = 0

Query: 134 WALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRLQRMADTWHRILLIP 193
           +++L ++ L+G+  GL    F   VH++ E       +E  ++L L      W    LI 
Sbjct: 31  FSVLFLSLLVGILAGLVGTYFEQAVHLVSETRTDWLKSEIGSFLPL------WLAAFLIS 90

Query: 194 VTGGVIVGMMHGLLEILGQIKQSSTSQRQGFDLLSGVFPT-------VKAIQAAITLGTG 253
                I   +  +     +   S   + +G   + G+ P        VK       LG+G
Sbjct: 91  AFLAFIGYFL--VHRFAPEAAGSGIPEIEG--AMDGMRPVRWWRVLPVKFFGGMGALGSG 150

Query: 254 CSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSFFAIE 313
             LG EGP+V +G +       +    +E  + + +AAGAA G+A+ FNA +AG  F IE
Sbjct: 151 MVLGREGPTVQMGGAVGRMISDIFRVKNEDTRHSLLAAGAAGGLAAAFNAPLAGIMFVIE 210

Query: 314 TVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAELPLYLILG 373
            +    R       +   +I+S+V ++ V  V+ G  +  T+P YD    + L L+L+LG
Sbjct: 211 EMRPQFR---YTLISVRAVIISAVAANIVFRVINGQDAVITMPQYDAPELSTLGLFLLLG 270

Query: 374 MLCGVVSVAVTRLVAWFSKLFDFIKERFGFPPVVCPALGGLGAGIIALKYPGILYWGFTN 433
            L GV  V    L+     LF           ++  ++ G   G++ L  P +   G + 
Sbjct: 271 ALFGVFGVLFNYLITLAQDLFVKFHRNDRKRYLLTGSMIGGCFGLLLLYVPELTGGGISL 330

Query: 434 VEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAVFGGS 493
           +  I + G      G  +L  +   ++  T LC GSG  GG++AP L +G   G  FG  
Sbjct: 331 IPTITNGGY-----GAGILLLLFVGRIFTTLLCFGSGAPGGIFAPMLALGTLFGYAFG-- 390

Query: 494 AVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILLPLM 545
               + A +       +P  +A+ GM A  A+    P+T +LL+ E+T +Y ++LPL+
Sbjct: 391 ----LIAKMWFPELNIEPGMFAIAGMGALFAATVRAPITGILLVIEMTNNYHLILPLI 424

BLAST of Carg21658-RA vs. ExPASy TrEMBL
Match: A0A6J1GCX9 (Chloride channel protein OS=Cucurbita moschata OX=3662 GN=LOC111453029 PE=3 SV=1)

HSP 1 Score: 1040.4 bits (2689), Expect = 2.6e-300
Identity = 543/548 (99.09%), Postives = 545/548 (99.45%), Query Frame = 0

Query: 1   MAEAEFSDQNRLLRSMEDHKGEDHDLESPDGNGIQPLRNNSGKRGVILDMFHHLNRGGSF 60
           MAEAEFSDQNRLLRSMEDHKGEDHDLES DGNGIQPLRNNSGKRGVILDMFHHLNRGGSF
Sbjct: 1   MAEAEFSDQNRLLRSMEDHKGEDHDLESQDGNGIQPLRNNSGKRGVILDMFHHLNRGGSF 60

Query: 61  SGRRLSYKRHDMDNHAATFSPTSLNIVGRARTA-SSSSSSSSDRHNNFNSYPLHAPTGSD 120
           SGRRLSYKRHDMDNHAATFSPTSLNIVGRARTA SSSSSSSSDRHNNFNSYPLHAPTGSD
Sbjct: 61  SGRRLSYKRHDMDNHAATFSPTSLNIVGRARTASSSSSSSSSDRHNNFNSYPLHAPTGSD 120

Query: 121 TEIDENVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRL 180
           TEIDENVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRL
Sbjct: 121 TEIDENVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRL 180

Query: 181 QRMADTWHRILLIPVTGGVIVGMMHGLLEILGQIKQSSTSQRQGFDLLSGVFPTVKAIQA 240
           QRMADTWHRILLIPVTGGVIVGMMHGLLEILGQIKQSSTSQRQGFDLLSGVFPTVKAIQA
Sbjct: 181 QRMADTWHRILLIPVTGGVIVGMMHGLLEILGQIKQSSTSQRQGFDLLSGVFPTVKAIQA 240

Query: 241 AITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVA 300
           AITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVA
Sbjct: 241 AITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVA 300

Query: 301 GSFFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAEL 360
           GSFFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAEL
Sbjct: 301 GSFFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAEL 360

Query: 361 PLYLILGMLCGVVSVAVTRLVAWFSKLFDFIKERFGFPPVVCPALGGLGAGIIALKYPGI 420
           PLYLILGMLCGVVSVAVTRLVAWFSKLFDFIKERFGFPPVVCPALGGLGAGIIALKYPGI
Sbjct: 361 PLYLILGMLCGVVSVAVTRLVAWFSKLFDFIKERFGFPPVVCPALGGLGAGIIALKYPGI 420

Query: 421 LYWGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAV 480
           LYWGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAV
Sbjct: 421 LYWGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAV 480

Query: 481 GAVFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRI 540
           GAVFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRI
Sbjct: 481 GAVFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRI 540

Query: 541 LLPLMASI 548
           LLPLM ++
Sbjct: 541 LLPLMGAV 548

BLAST of Carg21658-RA vs. ExPASy TrEMBL
Match: A0A6J1GCW3 (Chloride channel protein OS=Cucurbita moschata OX=3662 GN=LOC111453029 PE=3 SV=1)

HSP 1 Score: 1040.4 bits (2689), Expect = 2.6e-300
Identity = 543/548 (99.09%), Postives = 545/548 (99.45%), Query Frame = 0

Query: 1   MAEAEFSDQNRLLRSMEDHKGEDHDLESPDGNGIQPLRNNSGKRGVILDMFHHLNRGGSF 60
           MAEAEFSDQNRLLRSMEDHKGEDHDLES DGNGIQPLRNNSGKRGVILDMFHHLNRGGSF
Sbjct: 1   MAEAEFSDQNRLLRSMEDHKGEDHDLESQDGNGIQPLRNNSGKRGVILDMFHHLNRGGSF 60

Query: 61  SGRRLSYKRHDMDNHAATFSPTSLNIVGRARTA-SSSSSSSSDRHNNFNSYPLHAPTGSD 120
           SGRRLSYKRHDMDNHAATFSPTSLNIVGRARTA SSSSSSSSDRHNNFNSYPLHAPTGSD
Sbjct: 61  SGRRLSYKRHDMDNHAATFSPTSLNIVGRARTASSSSSSSSSDRHNNFNSYPLHAPTGSD 120

Query: 121 TEIDENVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRL 180
           TEIDENVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRL
Sbjct: 121 TEIDENVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRL 180

Query: 181 QRMADTWHRILLIPVTGGVIVGMMHGLLEILGQIKQSSTSQRQGFDLLSGVFPTVKAIQA 240
           QRMADTWHRILLIPVTGGVIVGMMHGLLEILGQIKQSSTSQRQGFDLLSGVFPTVKAIQA
Sbjct: 181 QRMADTWHRILLIPVTGGVIVGMMHGLLEILGQIKQSSTSQRQGFDLLSGVFPTVKAIQA 240

Query: 241 AITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVA 300
           AITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVA
Sbjct: 241 AITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVA 300

Query: 301 GSFFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAEL 360
           GSFFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAEL
Sbjct: 301 GSFFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAEL 360

Query: 361 PLYLILGMLCGVVSVAVTRLVAWFSKLFDFIKERFGFPPVVCPALGGLGAGIIALKYPGI 420
           PLYLILGMLCGVVSVAVTRLVAWFSKLFDFIKERFGFPPVVCPALGGLGAGIIALKYPGI
Sbjct: 361 PLYLILGMLCGVVSVAVTRLVAWFSKLFDFIKERFGFPPVVCPALGGLGAGIIALKYPGI 420

Query: 421 LYWGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAV 480
           LYWGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAV
Sbjct: 421 LYWGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAV 480

Query: 481 GAVFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRI 540
           GAVFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRI
Sbjct: 481 GAVFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRI 540

Query: 541 LLPLMASI 548
           LLPLM ++
Sbjct: 541 LLPLMGAV 548

BLAST of Carg21658-RA vs. ExPASy TrEMBL
Match: A0A6J1GCZ1 (Chloride channel protein OS=Cucurbita moschata OX=3662 GN=LOC111453029 PE=3 SV=1)

HSP 1 Score: 1040.4 bits (2689), Expect = 2.6e-300
Identity = 543/548 (99.09%), Postives = 545/548 (99.45%), Query Frame = 0

Query: 1   MAEAEFSDQNRLLRSMEDHKGEDHDLESPDGNGIQPLRNNSGKRGVILDMFHHLNRGGSF 60
           MAEAEFSDQNRLLRSMEDHKGEDHDLES DGNGIQPLRNNSGKRGVILDMFHHLNRGGSF
Sbjct: 1   MAEAEFSDQNRLLRSMEDHKGEDHDLESQDGNGIQPLRNNSGKRGVILDMFHHLNRGGSF 60

Query: 61  SGRRLSYKRHDMDNHAATFSPTSLNIVGRARTA-SSSSSSSSDRHNNFNSYPLHAPTGSD 120
           SGRRLSYKRHDMDNHAATFSPTSLNIVGRARTA SSSSSSSSDRHNNFNSYPLHAPTGSD
Sbjct: 61  SGRRLSYKRHDMDNHAATFSPTSLNIVGRARTASSSSSSSSSDRHNNFNSYPLHAPTGSD 120

Query: 121 TEIDENVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRL 180
           TEIDENVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRL
Sbjct: 121 TEIDENVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRL 180

Query: 181 QRMADTWHRILLIPVTGGVIVGMMHGLLEILGQIKQSSTSQRQGFDLLSGVFPTVKAIQA 240
           QRMADTWHRILLIPVTGGVIVGMMHGLLEILGQIKQSSTSQRQGFDLLSGVFPTVKAIQA
Sbjct: 181 QRMADTWHRILLIPVTGGVIVGMMHGLLEILGQIKQSSTSQRQGFDLLSGVFPTVKAIQA 240

Query: 241 AITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVA 300
           AITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVA
Sbjct: 241 AITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVA 300

Query: 301 GSFFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAEL 360
           GSFFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAEL
Sbjct: 301 GSFFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAEL 360

Query: 361 PLYLILGMLCGVVSVAVTRLVAWFSKLFDFIKERFGFPPVVCPALGGLGAGIIALKYPGI 420
           PLYLILGMLCGVVSVAVTRLVAWFSKLFDFIKERFGFPPVVCPALGGLGAGIIALKYPGI
Sbjct: 361 PLYLILGMLCGVVSVAVTRLVAWFSKLFDFIKERFGFPPVVCPALGGLGAGIIALKYPGI 420

Query: 421 LYWGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAV 480
           LYWGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAV
Sbjct: 421 LYWGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAV 480

Query: 481 GAVFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRI 540
           GAVFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRI
Sbjct: 481 GAVFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRI 540

Query: 541 LLPLMASI 548
           LLPLM ++
Sbjct: 541 LLPLMGAV 548

BLAST of Carg21658-RA vs. ExPASy TrEMBL
Match: A0A6J1KX22 (Chloride channel protein OS=Cucurbita maxima OX=3661 GN=LOC111498341 PE=3 SV=1)

HSP 1 Score: 1017.3 bits (2629), Expect = 2.4e-293
Identity = 533/547 (97.44%), Postives = 537/547 (98.17%), Query Frame = 0

Query: 1   MAEAEFSDQNRLLRSMEDHKGEDHDLESPDGNGIQPLRNNSGKRGVILDMFHHLNRGGSF 60
           MAEAEFSDQNRLLRSMEDHKGEDHDLES DGNGIQPLRNNSGKRG ILDMFHHLNRGGSF
Sbjct: 1   MAEAEFSDQNRLLRSMEDHKGEDHDLESQDGNGIQPLRNNSGKRGGILDMFHHLNRGGSF 60

Query: 61  SGRRLSYKRHDMDNHAATFSPTSLNIVGRARTASSSSSSSSDRHNNFNSYPLHAPTGSDT 120
           SGRRLS KRHDMDNHAATFSPTSLNIVGR RTA SSSSSSSDRHNNFNSY LHAPT SDT
Sbjct: 61  SGRRLSSKRHDMDNHAATFSPTSLNIVGRTRTA-SSSSSSSDRHNNFNSYSLHAPTVSDT 120

Query: 121 EIDENVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRLQ 180
           EID+NVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRLQ
Sbjct: 121 EIDDNVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRLQ 180

Query: 181 RMADTWHRILLIPVTGGVIVGMMHGLLEILGQIKQSSTSQRQGFDLLSGVFPTVKAIQAA 240
           RMADTWHRILLIPVTGGVIVGMMHGLLEIL QIKQSSTSQRQGFDLLSGVFPTVKAIQAA
Sbjct: 181 RMADTWHRILLIPVTGGVIVGMMHGLLEILDQIKQSSTSQRQGFDLLSGVFPTVKAIQAA 240

Query: 241 ITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAG 300
           ITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAG
Sbjct: 241 ITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAG 300

Query: 301 SFFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAELP 360
           SFFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAELP
Sbjct: 301 SFFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAELP 360

Query: 361 LYLILGMLCGVVSVAVTRLVAWFSKLFDFIKERFGFPPVVCPALGGLGAGIIALKYPGIL 420
           LYLILGMLCGVVSVAVTRLVAWFSKLF+FIKERFG PPVVCPALGGLGAGIIALKYPGIL
Sbjct: 361 LYLILGMLCGVVSVAVTRLVAWFSKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGIL 420

Query: 421 YWGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVG 480
           YWGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVG
Sbjct: 421 YWGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVG 480

Query: 481 AVFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRIL 540
           AVFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRIL
Sbjct: 481 AVFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRIL 540

Query: 541 LPLMASI 548
           LPLM ++
Sbjct: 541 LPLMGAV 546

BLAST of Carg21658-RA vs. ExPASy TrEMBL
Match: A0A6J1KYS2 (Chloride channel protein OS=Cucurbita maxima OX=3661 GN=LOC111498341 PE=3 SV=1)

HSP 1 Score: 1017.3 bits (2629), Expect = 2.4e-293
Identity = 533/547 (97.44%), Postives = 537/547 (98.17%), Query Frame = 0

Query: 1   MAEAEFSDQNRLLRSMEDHKGEDHDLESPDGNGIQPLRNNSGKRGVILDMFHHLNRGGSF 60
           MAEAEFSDQNRLLRSMEDHKGEDHDLES DGNGIQPLRNNSGKRG ILDMFHHLNRGGSF
Sbjct: 1   MAEAEFSDQNRLLRSMEDHKGEDHDLESQDGNGIQPLRNNSGKRGGILDMFHHLNRGGSF 60

Query: 61  SGRRLSYKRHDMDNHAATFSPTSLNIVGRARTASSSSSSSSDRHNNFNSYPLHAPTGSDT 120
           SGRRLS KRHDMDNHAATFSPTSLNIVGR RTA SSSSSSSDRHNNFNSY LHAPT SDT
Sbjct: 61  SGRRLSSKRHDMDNHAATFSPTSLNIVGRTRTA-SSSSSSSDRHNNFNSYSLHAPTVSDT 120

Query: 121 EIDENVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRLQ 180
           EID+NVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRLQ
Sbjct: 121 EIDDNVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRLQ 180

Query: 181 RMADTWHRILLIPVTGGVIVGMMHGLLEILGQIKQSSTSQRQGFDLLSGVFPTVKAIQAA 240
           RMADTWHRILLIPVTGGVIVGMMHGLLEIL QIKQSSTSQRQGFDLLSGVFPTVKAIQAA
Sbjct: 181 RMADTWHRILLIPVTGGVIVGMMHGLLEILDQIKQSSTSQRQGFDLLSGVFPTVKAIQAA 240

Query: 241 ITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAG 300
           ITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAG
Sbjct: 241 ITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAG 300

Query: 301 SFFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAELP 360
           SFFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAELP
Sbjct: 301 SFFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAELP 360

Query: 361 LYLILGMLCGVVSVAVTRLVAWFSKLFDFIKERFGFPPVVCPALGGLGAGIIALKYPGIL 420
           LYLILGMLCGVVSVAVTRLVAWFSKLF+FIKERFG PPVVCPALGGLGAGIIALKYPGIL
Sbjct: 361 LYLILGMLCGVVSVAVTRLVAWFSKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGIL 420

Query: 421 YWGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVG 480
           YWGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVG
Sbjct: 421 YWGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVG 480

Query: 481 AVFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRIL 540
           AVFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRIL
Sbjct: 481 AVFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRIL 540

Query: 541 LPLMASI 548
           LPLM ++
Sbjct: 541 LPLMGAV 546

BLAST of Carg21658-RA vs. TAIR 10
Match: AT1G55620.2 (chloride channel F )

HSP 1 Score: 724.5 bits (1869), Expect = 6.2e-209
Identity = 400/552 (72.46%), Postives = 449/552 (81.34%), Query Frame = 0

Query: 5   EFSDQNRLLRSMEDHK-----GE-DHDLESPDGNGIQPLRNNSGKRGVILDMFHHLNRGG 64
           E+++   LLRS +  +     GE D D+ES      Q     SG  GV  D+F H++R  
Sbjct: 8   EYNEDRHLLRSTDGDEVGIGGGEGDLDVES------QSPAIRSGAGGV-RDLFKHIDRRF 67

Query: 65  SFSGRRLSYKRHDMDNHAATFSPTSLNIVGRARTASSSSSSSSDRHNNFNSYPLHAPTGS 124
           S SGRRLS+KR  M+N              R   +SSS+ S++   +      LH+    
Sbjct: 68  SLSGRRLSFKR--MENIRVDRE--------RHNPSSSSAFSAAGEEDGGGISNLHSVDDR 127

Query: 125 DTEI---DENVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAA 184
           + E    +E + D+A PEWALLLI CL+G+A G+CVA FN GVHVIHEWAWAGTPNEGAA
Sbjct: 128 NDEYGFDEEVLGDSAPPEWALLLIGCLIGVAAGICVAGFNKGVHVIHEWAWAGTPNEGAA 187

Query: 185 WLRLQRMADTWHRILLIPVTGGVIVGMMHGLLEILGQIKQSSTSQRQGFDLLSGVFPTVK 244
           WLRLQR+ADTWHRILLIPVTGGVIVGMMHGLLEIL QI+QS++SQRQG D L+G++P +K
Sbjct: 188 WLRLQRLADTWHRILLIPVTGGVIVGMMHGLLEILDQIRQSNSSQRQGLDFLAGIYPVIK 247

Query: 245 AIQAAITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFN 304
           AIQAA+TLGTGCSLGPEGPSVDIGKSCANGF LMMENN E+ +IA  AAGAA+GIASGFN
Sbjct: 248 AIQAAVTLGTGCSLGPEGPSVDIGKSCANGFALMMENNRER-RIALTAAGAASGIASGFN 307

Query: 305 AAVAGSFFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKS 364
           AAVAG FFAIETVLRPLRAENSPPFTTAMIIL+SVISSTVS  LLGTQSAFTVP+YDLKS
Sbjct: 308 AAVAGCFFAIETVLRPLRAENSPPFTTAMIILASVISSTVSNALLGTQSAFTVPSYDLKS 367

Query: 365 AAELPLYLILGMLCGVVSVAVTRLVAWFSKLFDFIKERFGFPPVVCPALGGLGAGIIALK 424
           AAELPLYLILGMLCG VSV  +RLV WF+K FDFIK++FG P +VCPALGGLGAGIIALK
Sbjct: 368 AAELPLYLILGMLCGAVSVVFSRLVTWFTKSFDFIKDKFGLPAIVCPALGGLGAGIIALK 427

Query: 425 YPGILYWGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMI 484
           YPGILYWGFTNVEEILHTG   SAPG+WLL Q+AAAKVVATALCKGSGLVGGLYAPSLMI
Sbjct: 428 YPGILYWGFTNVEEILHTGKSASAPGIWLLAQLAAAKVVATALCKGSGLVGGLYAPSLMI 487

Query: 485 GAAVGAVFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTK 544
           GAAVGAVFGGSA E+IN AIPGNAAVAQPQAYALVGMAATLAS+CSVPLTSVLLLFELTK
Sbjct: 488 GAAVGAVFGGSAAEIINRAIPGNAAVAQPQAYALVGMAATLASMCSVPLTSVLLLFELTK 541

Query: 545 DYRILLPLMASI 548
           DYRILLPLM ++
Sbjct: 548 DYRILLPLMGAV 541

BLAST of Carg21658-RA vs. TAIR 10
Match: AT1G55620.1 (chloride channel F )

HSP 1 Score: 567.8 bits (1462), Expect = 9.6e-162
Identity = 296/346 (85.55%), Postives = 320/346 (92.49%), Query Frame = 0

Query: 202 MMHGLLEILGQIKQSSTSQRQGFDLLSGVFPTVKAIQAAITLGTGCSLGPEGPSVDIGKS 261
           MMHGLLEIL QI+QS++SQRQG D L+G++P +KAIQAA+TLGTGCSLGPEGPSVDIGKS
Sbjct: 1   MMHGLLEILDQIRQSNSSQRQGLDFLAGIYPVIKAIQAAVTLGTGCSLGPEGPSVDIGKS 60

Query: 262 CANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSFFAIETVLRPLRAENSPPFT 321
           CANGF LMMENN E+ +IA  AAGAA+GIASGFNAAVAG FFAIETVLRPLRAENSPPFT
Sbjct: 61  CANGFALMMENNRER-RIALTAAGAASGIASGFNAAVAGCFFAIETVLRPLRAENSPPFT 120

Query: 322 TAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAELPLYLILGMLCGVVSVAVTRLVA 381
           TAMIIL+SVISSTVS  LLGTQSAFTVP+YDLKSAAELPLYLILGMLCG VSV  +RLV 
Sbjct: 121 TAMIILASVISSTVSNALLGTQSAFTVPSYDLKSAAELPLYLILGMLCGAVSVVFSRLVT 180

Query: 382 WFSKLFDFIKERFGFPPVVCPALGGLGAGIIALKYPGILYWGFTNVEEILHTGNRPSAPG 441
           WF+K FDFIK++FG P +VCPALGGLGAGIIALKYPGILYWGFTNVEEILHTG   SAPG
Sbjct: 181 WFTKSFDFIKDKFGLPAIVCPALGGLGAGIIALKYPGILYWGFTNVEEILHTGKSASAPG 240

Query: 442 VWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAVFGGSAVEVINAAIPGNAAV 501
           +WLL Q+AAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAVFGGSA E+IN AIPGNAAV
Sbjct: 241 IWLLAQLAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAVFGGSAAEIINRAIPGNAAV 300

Query: 502 AQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILLPLMASI 548
           AQPQAYALVGMAATLAS+CSVPLTSVLLLFELTKDYRILLPLM ++
Sbjct: 301 AQPQAYALVGMAATLASMCSVPLTSVLLLFELTKDYRILLPLMGAV 345

BLAST of Carg21658-RA vs. TAIR 10
Match: AT4G35440.1 (chloride channel E )

HSP 1 Score: 353.6 bits (906), Expect = 2.9e-97
Identity = 210/451 (46.56%), Postives = 285/451 (63.19%), Query Frame = 0

Query: 110 YPLHAPTGSDTEIDENVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGT 169
           +P       +T+ DE   D    +   +  ACL+G+ TG+ V  FN  VH++ +++W G 
Sbjct: 50  FPRLPAAKQETDQDEVGFDQQPSQELAIASACLVGVLTGVSVVLFNNCVHLLRDFSWDGI 109

Query: 170 PNEGAAWLRLQRMADTWHRILLIPVTGGVIVGMMHGLLEILGQIKQSSTSQRQGFDLLSG 229
           P+ GA+WLR   +   W R++L+P  GG++V +++ L E  G   +S+       D +  
Sbjct: 110 PDRGASWLREAPIGSNWLRVILVPTIGGLVVSILNQLRESAG---KSTGDSHSSLDRVKA 169

Query: 230 VF-PTVKAIQAAITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAA 289
           V  P +K + A +TLGTG SLGPEGPSV+IG S A G   +  N S +   + +AAG+AA
Sbjct: 170 VLRPFLKTVAACVTLGTGNSLGPEGPSVEIGASIAKGVNSLF-NKSPQTGFSLLAAGSAA 229

Query: 290 GIASGFNAAVAGSFFAIETVLRPLRAENSP---PFTTAMIILSSVISSTVSTVLLGTQSA 349
           GI+SGFNAAVAG FFA+E+VL P  + +S    P TT+M+ILS+V +S VS + LG++ A
Sbjct: 230 GISSGFNAAVAGCFFAVESVLWPSSSTDSSTSLPNTTSMVILSAVTASVVSEIGLGSEPA 289

Query: 350 FTVPAYDLKSAAELPLYLILGMLCGVVSVAVTRLVAWFSKLFDFIKERFGFPPVVCPALG 409
           F VP YD +S  ELPLYL+LG LCG+VS+A++R  +  +   D + +  G P  V P +G
Sbjct: 290 FKVPDYDFRSPGELPLYLLLGALCGLVSLALSRCTSSMTSAVDSLNKDAGIPKAVFPVMG 349

Query: 410 GLGAGIIALKYPGILYWGFTNVEEILHTGNRPSAPGVW--LLTQIAAAKVVATALCKGSG 469
           GL  GIIAL YP +LYWGF NV+ +L    RP   G+   LL Q+ A K+ ATA C+ SG
Sbjct: 350 GLSVGIIALVYPEVLYWGFQNVDILLE--KRPFVKGLSADLLLQLVAVKIAATAWCRASG 409

Query: 470 LVGGLYAPSLMIGAAVGAVFGGSAVEVINAAIPGN-------AAVAQPQAYALVGMAATL 529
           LVGG YAPSL IG A G  +G    + I  A+  N         VA PQAY LVGMAATL
Sbjct: 410 LVGGYYAPSLFIGGAAGMAYG----KFIGLALAQNPDFNLSILEVASPQAYGLVGMAATL 469

Query: 530 ASVCSVPLTSVLLLFELTKDYRILLPLMASI 548
           A VC VPLT+VLLLFELT+DYRI+LPL+ ++
Sbjct: 470 AGVCQVPLTAVLLLFELTQDYRIVLPLLGAV 490

BLAST of Carg21658-RA vs. TAIR 10
Match: AT4G35440.2 (chloride channel E )

HSP 1 Score: 353.6 bits (906), Expect = 2.9e-97
Identity = 210/451 (46.56%), Postives = 285/451 (63.19%), Query Frame = 0

Query: 110 YPLHAPTGSDTEIDENVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGT 169
           +P       +T+ DE   D    +   +  ACL+G+ TG+ V  FN  VH++ +++W G 
Sbjct: 50  FPRLPAAKQETDQDEVGFDQQPSQELAIASACLVGVLTGVSVVLFNNCVHLLRDFSWDGI 109

Query: 170 PNEGAAWLRLQRMADTWHRILLIPVTGGVIVGMMHGLLEILGQIKQSSTSQRQGFDLLSG 229
           P+ GA+WLR   +   W R++L+P  GG++V +++ L E  G   +S+       D +  
Sbjct: 110 PDRGASWLREAPIGSNWLRVILVPTIGGLVVSILNQLRESAG---KSTGDSHSSLDRVKA 169

Query: 230 VF-PTVKAIQAAITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAA 289
           V  P +K + A +TLGTG SLGPEGPSV+IG S A G   +  N S +   + +AAG+AA
Sbjct: 170 VLRPFLKTVAACVTLGTGNSLGPEGPSVEIGASIAKGVNSLF-NKSPQTGFSLLAAGSAA 229

Query: 290 GIASGFNAAVAGSFFAIETVLRPLRAENSP---PFTTAMIILSSVISSTVSTVLLGTQSA 349
           GI+SGFNAAVAG FFA+E+VL P  + +S    P TT+M+ILS+V +S VS + LG++ A
Sbjct: 230 GISSGFNAAVAGCFFAVESVLWPSSSTDSSTSLPNTTSMVILSAVTASVVSEIGLGSEPA 289

Query: 350 FTVPAYDLKSAAELPLYLILGMLCGVVSVAVTRLVAWFSKLFDFIKERFGFPPVVCPALG 409
           F VP YD +S  ELPLYL+LG LCG+VS+A++R  +  +   D + +  G P  V P +G
Sbjct: 290 FKVPDYDFRSPGELPLYLLLGALCGLVSLALSRCTSSMTSAVDSLNKDAGIPKAVFPVMG 349

Query: 410 GLGAGIIALKYPGILYWGFTNVEEILHTGNRPSAPGVW--LLTQIAAAKVVATALCKGSG 469
           GL  GIIAL YP +LYWGF NV+ +L    RP   G+   LL Q+ A K+ ATA C+ SG
Sbjct: 350 GLSVGIIALVYPEVLYWGFQNVDILLE--KRPFVKGLSADLLLQLVAVKIAATAWCRASG 409

Query: 470 LVGGLYAPSLMIGAAVGAVFGGSAVEVINAAIPGN-------AAVAQPQAYALVGMAATL 529
           LVGG YAPSL IG A G  +G    + I  A+  N         VA PQAY LVGMAATL
Sbjct: 410 LVGGYYAPSLFIGGAAGMAYG----KFIGLALAQNPDFNLSILEVASPQAYGLVGMAATL 469

Query: 530 ASVCSVPLTSVLLLFELTKDYRILLPLMASI 548
           A VC VPLT+VLLLFELT+DYRI+LPL+ ++
Sbjct: 470 AGVCQVPLTAVLLLFELTQDYRIVLPLLGAV 490