Carg21320-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg21320-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	RING-H2 finger protein ATL46-like
Location	Carg_Chr15: 9310846 .. 9311355 (-)
Sequence length	510
RNA-Seq Expression	Carg21320-RA
Synteny	Carg21320-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGTTCTTCTGTTTCGCCATTAACGGCCACAGATGCTATCAAATCCTCCATTTCTTCACCAGAATCCTTCTCCATTTGAAGCTTCTCGCCGCCCGTTTCGGCCTCCTCAAGCCCTCGCCGGAGCACCATGCCGACTCTCCCTTCCAGCCTCCGACCAGTTACCTCTTCCTCACCGACGACCCCTGCCCTTCGCTGGTCACCGTTCCGGCGGCTGATTTGCAGGCCAGGATCAAGAACAGCCTCCCAATCGTCGAGTTTGGGGATTTTGCTGCAAAACAGGGGATTGAATTTGGGGGAAATGACGGAGAAGAAGAGAATCGGATGTGTACGATCTGTTTGAGTGAAATGGAGAGATCGGAGCCGATGAGAGAGCTCTGTAATTGCCGCCATGTCTTCCATCGGGAGTGTTTGGATACGTGGATTGATGAGTTTCAGATTACGTGCCCTGTTTGTAGATCTTTTCTTTTGCCCTCCGGTGACGGCGGTGGGTTTCGATTCCTCCGGTGA

mRNA sequence

ATGGGGTTCTTCTGTTTCGCCATTAACGGCCACAGATGCTATCAAATCCTCCATTTCTTCACCAGAATCCTTCTCCATTTGAAGCTTCTCGCCGCCCGTTTCGGCCTCCTCAAGCCCTCGCCGGAGCACCATGCCGACTCTCCCTTCCAGCCTCCGACCAGTTACCTCTTCCTCACCGACGACCCCTGCCCTTCGCTGGTCACCGTTCCGGCGGCTGATTTGCAGGCCAGGATCAAGAACAGCCTCCCAATCGTCGAGTTTGGGGATTTTGCTGCAAAACAGGGGATTGAATTTGGGGGAAATGACGGAGAAGAAGAGAATCGGATGTGTACGATCTGTTTGAGTGAAATGGAGAGATCGGAGCCGATGAGAGAGCTCTGTAATTGCCGCCATGTCTTCCATCGGGAGTGTTTGGATACGTGGATTGATGAGTTTCAGATTACGTGCCCTGTTTGTAGATCTTTTCTTTTGCCCTCCGGTGACGGCGGTGGGTTTCGATTCCTCCGGTGA

Coding sequence (CDS)

ATGGGGTTCTTCTGTTTCGCCATTAACGGCCACAGATGCTATCAAATCCTCCATTTCTTCACCAGAATCCTTCTCCATTTGAAGCTTCTCGCCGCCCGTTTCGGCCTCCTCAAGCCCTCGCCGGAGCACCATGCCGACTCTCCCTTCCAGCCTCCGACCAGTTACCTCTTCCTCACCGACGACCCCTGCCCTTCGCTGGTCACCGTTCCGGCGGCTGATTTGCAGGCCAGGATCAAGAACAGCCTCCCAATCGTCGAGTTTGGGGATTTTGCTGCAAAACAGGGGATTGAATTTGGGGGAAATGACGGAGAAGAAGAGAATCGGATGTGTACGATCTGTTTGAGTGAAATGGAGAGATCGGAGCCGATGAGAGAGCTCTGTAATTGCCGCCATGTCTTCCATCGGGAGTGTTTGGATACGTGGATTGATGAGTTTCAGATTACGTGCCCTGTTTGTAGATCTTTTCTTTTGCCCTCCGGTGACGGCGGTGGGTTTCGATTCCTCCGGTGA

Protein sequence

MGFFCFAINGHRCYQILHFFTRILLHLKLLAARFGLLKPSPEHHADSPFQPPTSYLFLTDDPCPSLVTVPAADLQARIKNSLPIVEFGDFAAKQGIEFGGNDGEEENRMCTICLSEMERSEPMRELCNCRHVFHRECLDTWIDEFQITCPVCRSFLLPSGDGGGFRFLR

Homology

[Close]

BLAST of Carg21320-RA vs. NCBI nr
Match: XP_023520502.1 (RING-H2 finger protein ATL2-like [Cucurbita pepo subsp. pepo] >KAG7017057.1 RING-H2 finger protein ATL2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 363.2 bits (931), Expect = 1.2e-96
Identity = 169/169 (100.00%), Postives = 169/169 (100.00%), Query Frame = 0


Query: 1   MGFFCFAINGHRCYQILHFFTRILLHLKLLAARFGLLKPSPEHHADSPFQPPTSYLFLTD 60
           MGFFCFAINGHRCYQILHFFTRILLHLKLLAARFGLLKPSPEHHADSPFQPPTSYLFLTD
Sbjct: 1   MGFFCFAINGHRCYQILHFFTRILLHLKLLAARFGLLKPSPEHHADSPFQPPTSYLFLTD 60

Query: 61  DPCPSLVTVPAADLQARIKNSLPIVEFGDFAAKQGIEFGGNDGEEENRMCTICLSEMERS 120
           DPCPSLVTVPAADLQARIKNSLPIVEFGDFAAKQGIEFGGNDGEEENRMCTICLSEMERS
Sbjct: 61  DPCPSLVTVPAADLQARIKNSLPIVEFGDFAAKQGIEFGGNDGEEENRMCTICLSEMERS 120

Query: 121 EPMRELCNCRHVFHRECLDTWIDEFQITCPVCRSFLLPSGDGGGFRFLR 170
           EPMRELCNCRHVFHRECLDTWIDEFQITCPVCRSFLLPSGDGGGFRFLR
Sbjct: 121 EPMRELCNCRHVFHRECLDTWIDEFQITCPVCRSFLLPSGDGGGFRFLR 169

[Close]

BLAST of Carg21320-RA vs. NCBI nr
Match: XP_038907243.1 (RING-H2 finger protein ATL2-like [Benincasa hispida])

HSP 1 Score: 267.3 bits (682), Expect = 8.9e-68
Identity = 127/169 (75.15%), Postives = 140/169 (82.84%), Query Frame = 0


Query: 1   MGFFCFAINGHRCYQILHFFTRILLHLKLLAARFGLLKPSPEHHADSPFQPPTSYLFLTD 60
           MGF  F+IN H C QILHFFT ILL LKLLAA F LLK SPEH+ADSPF  PTS++FLT+
Sbjct: 1   MGFLYFSINAHLCSQILHFFTTILLQLKLLAAHFRLLKLSPEHNADSPFDRPTSFVFLTE 60

Query: 61  DPCPSLVTVPAADLQARIKNSLPIVEFGDFAAKQGIEFGGNDGEEENRMCTICLSEMERS 120
            PC SLVTVPAADLQ RIK SLPIV F DF AKQG+EFG N+ +EENR+CT+CL EMERS
Sbjct: 61  GPCSSLVTVPAADLQGRIKKSLPIVAFRDFIAKQGMEFGENEEDEENRICTVCLCEMERS 120

Query: 121 EPMRELCNCRHVFHRECLDTWIDEFQITCPVCRSFLLPSGDGGGFRFLR 170
           E MREL NC HVFHREC D WIDEFQITCPVCRS LLP+G+GGGFRF +
Sbjct: 121 ESMRELPNCCHVFHRECFDAWIDEFQITCPVCRSLLLPAGEGGGFRFFQ 169

[Close]

BLAST of Carg21320-RA vs. NCBI nr
Match: XP_022996076.1 (RING-H2 finger protein ATL46-like [Cucurbita maxima])

HSP 1 Score: 239.2 bits (609), Expect = 2.6e-59
Identity = 119/170 (70.00%), Postives = 131/170 (77.06%), Query Frame = 0


Query: 1   MGFFCFAINGHRCYQILH-FFTRILLHLKLLAARFGLLKPSPEHHADSPFQPPTSYLFLT 60
           MGF  FAIN     QILH  FTRILL  KLLAA FGL+KPSPE  ADSPF+ PTSY+ LT
Sbjct: 1   MGFLHFAINAQTSSQILHQAFTRILLQFKLLAAHFGLIKPSPELVADSPFERPTSYVLLT 60

Query: 61  DDPCPSLVTVPAADLQARIKNSLPIVEFGDFAAKQGIEFGGNDGEEENRMCTICLSEMER 120
           +   PSLVTVPA DLQ RIK SLPI+E+GDF  KQG     N  +EENR CTICL EMER
Sbjct: 61  EGRYPSLVTVPAVDLQVRIKTSLPIIEYGDFVEKQGRGSPENKVDEENRKCTICLCEMER 120

Query: 121 SEPMRELCNCRHVFHRECLDTWIDEFQITCPVCRSFLLPSGDGGGFRFLR 170
           SE MRELCNC HVFHR+CLDTWIDEF+ITCPVCRS L P+G+G GF F+R
Sbjct: 121 SETMRELCNCCHVFHRDCLDTWIDEFRITCPVCRSMLFPAGEGDGFPFVR 170

[Close]

BLAST of Carg21320-RA vs. NCBI nr
Match: XP_022159502.1 (RING-H2 finger protein ATL2-like [Momordica charantia])

HSP 1 Score: 228.8 bits (582), Expect = 3.5e-56
Identity = 114/175 (65.14%), Postives = 130/175 (74.29%), Query Frame = 0


Query: 1   MGFFCFAINGHRCYQILHFFTRILLHLKLLAARFGLLKPSPE--HHADSPFQPPTSYLFL 60
           MGF  FAINGHRC QILHFFT I   LK L    G+LKP P+    AD     PTSY+F+
Sbjct: 1   MGFLYFAINGHRCSQILHFFTGIFRRLKHLGVYLGVLKPPPQQPEDADDRVDRPTSYVFV 60

Query: 61  TDDPCPSLVTVPAADLQARIKNSLPIVEFGDFAAKQGIEFGGN----DGEEENRMCTICL 120
           T+  CPSLVTVPA DLQARIK SLPIVEFGDFAAK  ++F  +    + EEE   CTICL
Sbjct: 61  TEGLCPSLVTVPATDLQARIKKSLPIVEFGDFAAKGELKFWEDWEEEEEEEEKGRCTICL 120

Query: 121 SEMERSEPMRELCNCRHVFHRECLDTWIDEFQITCPVCRSFLLPSGDGGGFRFLR 170
           S+ME+SE MREL NC HVFH+ECLDTWIDEFQITCP+CRS LLP+G+G G R+ R
Sbjct: 121 SKMEKSEKMRELSNCCHVFHKECLDTWIDEFQITCPICRSMLLPAGEGDGLRYFR 175

[Close]

BLAST of Carg21320-RA vs. NCBI nr
Match: XP_023533023.1 (RING-H2 finger protein ATL2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 225.3 bits (573), Expect = 3.9e-55
Identity = 111/154 (72.08%), Postives = 121/154 (78.57%), Query Frame = 0


Query: 1   MGFFCFAINGHRCYQILHFFTRILLHLKLLAARFGLLKPSPEHHADSPFQPPTSYLFLTD 60
           MGF  FAING R  QILH FTRILL  KLLAA FGL+KPSPEH A+SPF+ PTSY+ LT+
Sbjct: 1   MGFLHFAINGQRSSQILHLFTRILLQFKLLAAHFGLIKPSPEHVAESPFERPTSYVLLTE 60

Query: 61  DPCPSLVTVPAADLQARIKNSLPIVEFGDFAAKQGIEFGGNDGEEENRMCTICLSEMERS 120
              PSLVTVPA DLQARIK SLPI+E+GDF  KQG        +EENR CTICL EMERS
Sbjct: 61  GRYPSLVTVPAVDLQARIKTSLPIIEYGDFVEKQGRGSPEEKVDEENRKCTICLCEMERS 120

Query: 121 EPMRELCNCRHVFHRECLDTWIDEFQITCPVCRS 155
           E MREL NC HVFH +CLDTWIDEF+ITCPVCRS
Sbjct: 121 ETMRELSNCCHVFHPDCLDTWIDEFRITCPVCRS 154

[Close]

BLAST of Carg21320-RA vs. ExPASy Swiss-Prot
Match: Q9XF92 (Brassinosteroid-responsive RING protein 1 OS=Arabidopsis thaliana OX=3702 GN=BRH1 PE=2 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 3.8e-13
Identity = 33/81 (40.74%), Postives = 47/81 (58.02%), Query Frame = 0


Query: 78  IKNSLPIVEFGDFAAKQGIEFGGNDGEEENRMCTICLSEMERSEPMRELCNCRHVFHREC 137
           I+  LP+++F +           N GE+    C +CL E E  + +R L NCRH+FHR C
Sbjct: 71  IREILPVIKFEELT---------NSGEDLPENCAVCLYEFEGEQEIRWLRNCRHIFHRSC 130

Query: 138 LDTWIDEFQITCPVCRSFLLP 159
           LD W+D  Q TCP+CR+  +P
Sbjct: 131 LDRWMDHDQKTCPLCRTPFVP 142

[Close]

BLAST of Carg21320-RA vs. ExPASy Swiss-Prot
Match: Q9ZT50 (E3 ubiquitin-protein ligase RHA2A OS=Arabidopsis thaliana OX=3702 GN=RHA2A PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 3.0e-10
Identity = 27/59 (45.76%), Postives = 41/59 (69.49%), Query Frame = 0


Query: 99  GGNDGEEENRMCTICLSEMERSEPMRELCNCRHVFHRECLDTWIDEFQITCPVCRSFLL 158
           GG  G +    C +CLS+++  E +R+L  CRHVFH++CL+ W+ +F  TCP+CRS L+
Sbjct: 79  GGGGGSD----CVVCLSKLKEGEEVRKL-ECRHVFHKKCLEGWLHQFNFTCPLCRSALV 132

[Close]

BLAST of Carg21320-RA vs. ExPASy Swiss-Prot
Match: Q9SUS4 (Probable E3 ubiquitin-protein ligase RHA1A OS=Arabidopsis thaliana OX=3702 GN=RHA1A PE=2 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 8.8e-10
Identity = 36/94 (38.30%), Postives = 49/94 (52.13%), Query Frame = 0


Query: 67  VTVPAADLQARIKNSL-PIVEFGDFAAKQGIEFGGNDGEEENRMCTICLSEMERSEPMRE 126
           +T  A    A + N L P+V F D            D E+    CT+CLS+ E  + +R+
Sbjct: 54  LTRHALSTSASLANELIPVVRFSDLP---------TDPED---CCTVCLSDFESDDKVRQ 113

Query: 127 LCNCRHVFHRECLDTWIDEF-QITCPVCRSFLLP 159
           L  C HVFH  CLD WI ++ ++ CPVCR   LP
Sbjct: 114 LPKCGHVFHHYCLDRWIVDYNKMKCPVCRHRFLP 135

[Close]

BLAST of Carg21320-RA vs. ExPASy Swiss-Prot
Match: Q9ZU51 (E3 ubiquitin-protein ligase RHA2B OS=Arabidopsis thaliana OX=3702 GN=RHA2B PE=1 SV=2)

HSP 1 Score: 64.7 bits (156), Expect = 1.2e-09
Identity = 24/49 (48.98%), Postives = 36/49 (73.47%), Query Frame = 0


Query: 110 CTICLSEMERSEPMRELCNCRHVFHRECLDTWIDEFQITCPVCRSFLLP 159
           C +CLS+++  E +R+L +CRHVFH++CL+ W+      CP+CRS LLP
Sbjct: 74  CIVCLSKLKTGEEVRKL-DCRHVFHKQCLEGWLQHLNFNCPLCRSPLLP 121

[Close]

BLAST of Carg21320-RA vs. ExPASy Swiss-Prot
Match: Q9FL07 (RING-H2 finger protein ATL46 OS=Arabidopsis thaliana OX=3702 GN=ATL46 PE=2 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 1.5e-09
Identity = 43/142 (30.28%), Postives = 60/142 (42.25%), Query Frame = 0


Query: 19  FFTRILLHLKLLAARFGLLKPSPEHHADSPFQPPTSYLFLTDDPCPSLVTVPAADLQARI 78
           FF   LLH   L  RF +  PS    + S   P  S           L  +  + L    
Sbjct: 56  FFISGLLH---LLVRFLIKHPSATASSRSNRFPEISTSDALQRQLQQLFHLNDSGLDQAF 115

Query: 79  KNSLPIVEFGDFAAKQGIEFGGNDGEEENRMCTICLSEMERSEPMRELCNCRHVFHRECL 138
            ++LP+  + +     G   GGN   +E   C +CL E    + +R L  C H FH  C+
Sbjct: 116 IDALPVFHYKEIVGSAG-GGGGNGAAQEPFDCAVCLCEFSEKDKLRLLPMCSHAFHLNCI 175

Query: 139 DTWIDEFQITCPVCRSFLLPSG 161
           DTW+ +   TCP+CR  L   G
Sbjct: 176 DTWL-QSNSTCPLCRGTLFSPG 192

[Close]

BLAST of Carg21320-RA vs. ExPASy TrEMBL
Match: A0A6J1K3P6 (RING-H2 finger protein ATL46-like OS=Cucurbita maxima OX=3661 GN=LOC111491392 PE=4 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 1.3e-59
Identity = 119/170 (70.00%), Postives = 131/170 (77.06%), Query Frame = 0


Query: 1   MGFFCFAINGHRCYQILH-FFTRILLHLKLLAARFGLLKPSPEHHADSPFQPPTSYLFLT 60
           MGF  FAIN     QILH  FTRILL  KLLAA FGL+KPSPE  ADSPF+ PTSY+ LT
Sbjct: 1   MGFLHFAINAQTSSQILHQAFTRILLQFKLLAAHFGLIKPSPELVADSPFERPTSYVLLT 60

Query: 61  DDPCPSLVTVPAADLQARIKNSLPIVEFGDFAAKQGIEFGGNDGEEENRMCTICLSEMER 120
           +   PSLVTVPA DLQ RIK SLPI+E+GDF  KQG     N  +EENR CTICL EMER
Sbjct: 61  EGRYPSLVTVPAVDLQVRIKTSLPIIEYGDFVEKQGRGSPENKVDEENRKCTICLCEMER 120

Query: 121 SEPMRELCNCRHVFHRECLDTWIDEFQITCPVCRSFLLPSGDGGGFRFLR 170
           SE MRELCNC HVFHR+CLDTWIDEF+ITCPVCRS L P+G+G GF F+R
Sbjct: 121 SETMRELCNCCHVFHRDCLDTWIDEFRITCPVCRSMLFPAGEGDGFPFVR 170

[Close]

BLAST of Carg21320-RA vs. ExPASy TrEMBL
Match: A0A6J1E009 (RING-H2 finger protein ATL2-like OS=Momordica charantia OX=3673 GN=LOC111025900 PE=4 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 1.7e-56
Identity = 114/175 (65.14%), Postives = 130/175 (74.29%), Query Frame = 0


Query: 1   MGFFCFAINGHRCYQILHFFTRILLHLKLLAARFGLLKPSPE--HHADSPFQPPTSYLFL 60
           MGF  FAINGHRC QILHFFT I   LK L    G+LKP P+    AD     PTSY+F+
Sbjct: 1   MGFLYFAINGHRCSQILHFFTGIFRRLKHLGVYLGVLKPPPQQPEDADDRVDRPTSYVFV 60

Query: 61  TDDPCPSLVTVPAADLQARIKNSLPIVEFGDFAAKQGIEFGGN----DGEEENRMCTICL 120
           T+  CPSLVTVPA DLQARIK SLPIVEFGDFAAK  ++F  +    + EEE   CTICL
Sbjct: 61  TEGLCPSLVTVPATDLQARIKKSLPIVEFGDFAAKGELKFWEDWEEEEEEEEKGRCTICL 120

Query: 121 SEMERSEPMRELCNCRHVFHRECLDTWIDEFQITCPVCRSFLLPSGDGGGFRFLR 170
           S+ME+SE MREL NC HVFH+ECLDTWIDEFQITCP+CRS LLP+G+G G R+ R
Sbjct: 121 SKMEKSEKMRELSNCCHVFHKECLDTWIDEFQITCPICRSMLLPAGEGDGLRYFR 175

[Close]

BLAST of Carg21320-RA vs. ExPASy TrEMBL
Match: A0A2Z7BRP5 (RING-type domain-containing protein OS=Dorcoceras hygrometricum OX=472368 GN=F511_14687 PE=4 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 9.1e-26
Identity = 69/173 (39.88%), Postives = 96/173 (55.49%), Query Frame = 0


Query: 1   MGFFCFAINGHRCYQILHFFTR--ILLHLKLL----AARFGLLKPSPEHHADSPFQPPTS 60
           MGF   AI      ++   FT   IL HL+ L      R G+ KP PE   + P     +
Sbjct: 1   MGFLFHAIK----IKLPALFTSLDILTHLRFLFTIALTRLGIYKPPPEQQQEDPNSRENN 60

Query: 61  YLFLTDDPCPSLVTVPAADLQARIKNSLPIVEFGDFAAKQGIEFGGNDGEEENRMCTICL 120
           Y+F+ D   PSLV +P   + A IK  +PI+++ D A + G E G    + +  +CT+CL
Sbjct: 61  YIFILDGSSPSLVPIPVHTVTAAIKKKVPIIQYRDCAQRLG-EMG---SDSDQMVCTVCL 120

Query: 121 SEMERSEPMRELCNCRHVFHRECLDTWIDEFQITCPVCRSFLLPSGDGGGFRF 168
             +  S+ +REL NC HVFHR+CLD W+DE Q+TCP+CRS LLP      + F
Sbjct: 121 ECVGLSDEIRELSNCSHVFHRDCLDAWVDEGQVTCPMCRSILLPRPPNSKYAF 165

[Close]

BLAST of Carg21320-RA vs. ExPASy TrEMBL
Match: A0A2G9G363 (RING-type domain-containing protein OS=Handroanthus impetiginosus OX=429701 GN=CDL12_27874 PE=4 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 1.6e-25
Identity = 68/161 (42.24%), Postives = 91/161 (56.52%), Query Frame = 0


Query: 1   MGFFCFAINGHRCYQI---LHFFTRILLHLKLLAARFGLLKPSPEHHADSPFQPPTSYLF 60
           MGF  +AI   +   I   L+ FT             GL KP PE  ++S      +Y+ 
Sbjct: 1   MGFLYYAIKIPKAITITFFLNIFTNARFFFIGALTHLGLYKPPPEEESNSR-DNSNNYIL 60

Query: 61  LTDDPCPSLVTVPAADLQARIKNSLPIVEFGDFAAKQGIEFGGNDGEEENRMCTICLSEM 120
           + D   PSLV VP   +   IKN +PI+++ DF  +   E  G +G    ++C+ICL  +
Sbjct: 61  ILDGSSPSLVPVPVQVVTTAIKNRVPIIQYHDFLLRHK-EEEGEEGLCGEKVCSICLECI 120

Query: 121 ERSEPMRELCNCRHVFHRECLDTWIDEFQITCPVCRSFLLP 159
           E    +RELCNC H+FHRECLDTWIDE Q+TCP+CRS LLP
Sbjct: 121 EMKHEIRELCNCSHLFHRECLDTWIDEGQVTCPLCRSMLLP 159

[Close]

BLAST of Carg21320-RA vs. ExPASy TrEMBL
Match: A0A1R3K6P6 (Zinc finger, RING-type OS=Corchorus olitorius OX=93759 GN=COLO4_10837 PE=4 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 2.0e-25
Identity = 67/142 (47.18%), Postives = 84/142 (59.15%), Query Frame = 0


Query: 19  FFTRILLHLKLLAARFGLLKPSPEHHADSPFQPP-TSYLFLTDDPCPSLVTVPAADLQAR 78
           FF     HL       GLLKP      +  F  P    + LTD    SLV VP   + A 
Sbjct: 23  FFREAFTHL-------GLLKPPAAASEEEGFYSPFNGIILLTDSRSGSLVPVPTQLVTAM 82

Query: 79  IKNSLPIVEFGDFAAKQGIEFGGNDGEEEN-RMCTICLSEMERSEPMRELCNCRHVFHRE 138
           IK  +P+VE+G+F  + G E   +D +  N R+CT+CL  ME ++ MRELCNC HVFHRE
Sbjct: 83  IKKKVPVVEYGNFMERFGDEL--DDEQLANCRVCTVCLESMEENDKMRELCNCSHVFHRE 142

Query: 139 CLDTWIDEFQITCPVCRSFLLP 159
           CLDTW+DE Q+TCP+CRS L P
Sbjct: 143 CLDTWVDEGQVTCPLCRSTLYP 155

[Close]

BLAST of Carg21320-RA vs. TAIR 10
Match: AT3G61460.1 (brassinosteroid-responsive RING-H2 )

HSP 1 Score: 76.3 bits (186), Expect = 2.7e-14
Identity = 33/81 (40.74%), Postives = 47/81 (58.02%), Query Frame = 0


Query: 78  IKNSLPIVEFGDFAAKQGIEFGGNDGEEENRMCTICLSEMERSEPMRELCNCRHVFHREC 137
           I+  LP+++F +           N GE+    C +CL E E  + +R L NCRH+FHR C
Sbjct: 71  IREILPVIKFEELT---------NSGEDLPENCAVCLYEFEGEQEIRWLRNCRHIFHRSC 130

Query: 138 LDTWIDEFQITCPVCRSFLLP 159
           LD W+D  Q TCP+CR+  +P
Sbjct: 131 LDRWMDHDQKTCPLCRTPFVP 142

[Close]

BLAST of Carg21320-RA vs. TAIR 10
Match: AT1G63840.1 (RING/U-box superfamily protein )

HSP 1 Score: 72.0 bits (175), Expect = 5.1e-13
Identity = 36/99 (36.36%), Postives = 52/99 (52.53%), Query Frame = 0


Query: 61  DPCPSLVTVPAADLQARIKNSLPIVEFGDFAAKQGIEFGGNDGEEENRMCTICLSEMERS 120
           DP P+L    +A + A     LP+V F D                E+  C +CL + E  
Sbjct: 54  DPPPTLTKPDSAAILA--GEMLPVVRFSDI------------NRPESECCAVCLYDFEND 113

Query: 121 EPMRELCNCRHVFHRECLDTWIDEF-QITCPVCRSFLLP 159
           + +R L NCRH+FHR CLD W+  + Q+TCP+CR+  +P
Sbjct: 114 DEIRRLTNCRHIFHRGCLDRWMMGYNQMTCPLCRTQFIP 138

[Close]

BLAST of Carg21320-RA vs. TAIR 10
Match: AT3G43430.1 (RING/U-box superfamily protein )

HSP 1 Score: 71.6 bits (174), Expect = 6.7e-13
Identity = 35/90 (38.89%), Postives = 48/90 (53.33%), Query Frame = 0


Query: 78  IKNSLPIVEFGDFAAKQGIEFGGNDGEEENRMCTICLSEMERSEPMRELCNCRHVFHREC 137
           IK SL +  F D   +             N MC +CL ++E  + +REL NC HVFHR+C
Sbjct: 59  IKESLAVSAFRDAVERSPAAI--------NDMCAVCLGDLEDEDEIRELRNCTHVFHRDC 118

Query: 138 LDTWI--------DEFQITCPVCRSFLLPS 160
           +D W+        D+   TCP+CR+ LLPS
Sbjct: 119 IDRWLDYECCGGDDDNHRTCPLCRTPLLPS 140

[Close]

BLAST of Carg21320-RA vs. TAIR 10
Match: AT1G15100.1 (RING-H2 finger A2A )

HSP 1 Score: 66.6 bits (161), Expect = 2.2e-11
Identity = 27/59 (45.76%), Postives = 41/59 (69.49%), Query Frame = 0


Query: 99  GGNDGEEENRMCTICLSEMERSEPMRELCNCRHVFHRECLDTWIDEFQITCPVCRSFLL 158
           GG  G +    C +CLS+++  E +R+L  CRHVFH++CL+ W+ +F  TCP+CRS L+
Sbjct: 79  GGGGGSD----CVVCLSKLKEGEEVRKL-ECRHVFHKKCLEGWLHQFNFTCPLCRSALV 132

[Close]

BLAST of Carg21320-RA vs. TAIR 10
Match: AT5G20885.1 (RING/U-box superfamily protein )

HSP 1 Score: 65.1 bits (157), Expect = 6.3e-11
Identity = 36/102 (35.29%), Postives = 50/102 (49.02%), Query Frame = 0


Query: 70  PAADLQARIKNSLPIVEFGDFAAKQGIEFGGNDGEEENRMCTICLSEMERSEPMRELCNC 129
           P+   QA IK SL +  F D A +             N  C +CL ++E  + +REL NC
Sbjct: 51  PSISSQA-IKESLSVTTFRDAAERSPAMI--------NDTCAVCLGDLEDGDEVRELRNC 110

Query: 130 RHVFHRECLDTWID--------------EFQITCPVCRSFLL 158
            H+FHREC+D W+D              +   TCP+CR+ LL
Sbjct: 111 SHMFHRECIDRWLDYECCGGDENNEGEEDNHRTCPLCRTPLL 143

The following BLAST results are available for this feature:

BLAST of Carg21320-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023520502.1	1.2e-96	100.00	RING-H2 finger protein ATL2-like [Cucurbita pepo subsp. pepo] >KAG7017057.1 RING...	[more]
XP_038907243.1	8.9e-68	75.15	RING-H2 finger protein ATL2-like [Benincasa hispida]	[more]
XP_022996076.1	2.6e-59	70.00	RING-H2 finger protein ATL46-like [Cucurbita maxima]	[more]
XP_022159502.1	3.5e-56	65.14	RING-H2 finger protein ATL2-like [Momordica charantia]	[more]
XP_023533023.1	3.9e-55	72.08	RING-H2 finger protein ATL2-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg21320-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XF92	3.8e-13	40.74	Brassinosteroid-responsive RING protein 1 OS=Arabidopsis thaliana OX=3702 GN=BRH...	[more]
Q9ZT50	3.0e-10	45.76	E3 ubiquitin-protein ligase RHA2A OS=Arabidopsis thaliana OX=3702 GN=RHA2A PE=1 ...	[more]
Q9SUS4	8.8e-10	38.30	Probable E3 ubiquitin-protein ligase RHA1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q9ZU51	1.2e-09	48.98	E3 ubiquitin-protein ligase RHA2B OS=Arabidopsis thaliana OX=3702 GN=RHA2B PE=1 ...	[more]
Q9FL07	1.5e-09	30.28	RING-H2 finger protein ATL46 OS=Arabidopsis thaliana OX=3702 GN=ATL46 PE=2 SV=1	[more]

BLAST of Carg21320-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K3P6	1.3e-59	70.00	RING-H2 finger protein ATL46-like OS=Cucurbita maxima OX=3661 GN=LOC111491392 PE...	[more]
A0A6J1E009	1.7e-56	65.14	RING-H2 finger protein ATL2-like OS=Momordica charantia OX=3673 GN=LOC111025900 ...	[more]
A0A2Z7BRP5	9.1e-26	39.88	RING-type domain-containing protein OS=Dorcoceras hygrometricum OX=472368 GN=F51...	[more]
A0A2G9G363	1.6e-25	42.24	RING-type domain-containing protein OS=Handroanthus impetiginosus OX=429701 GN=C...	[more]
A0A1R3K6P6	2.0e-25	47.18	Zinc finger, RING-type OS=Corchorus olitorius OX=93759 GN=COLO4_10837 PE=4 SV=1	[more]

BLAST of Carg21320-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G61460.1	2.7e-14	40.74	brassinosteroid-responsive RING-H2	[more]
AT1G63840.1	5.1e-13	36.36	RING/U-box superfamily protein	[more]
AT3G43430.1	6.7e-13	38.89	RING/U-box superfamily protein	[more]
AT1G15100.1	2.2e-11	45.76	RING-H2 finger A2A	[more]
AT5G20885.1	6.3e-11	35.29	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 110..152 e-value: 3.4E-5 score: 33.3
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 109..153 e-value: 1.8E-11 score: 44.2
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 110..153 score: 12.754832
IPR011016	Zinc finger, RING-CH-type	SMART	SM00744	ringv_2	coord: 109..153 e-value: 1.1E-4 score: 24.1
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 73..157 e-value: 1.3E-16 score: 62.0
None	No IPR available	PANTHER	PTHR45969:SF35	RING-H2 ZINC FINGER PROTEIN RHA4A-LIKE	coord: 17..159
None	No IPR available	PANTHER	PTHR45969	RING ZINC FINGER PROTEIN-RELATED	coord: 17..159
None	No IPR available	CDD	cd16448	RING-H2	coord: 110..153 e-value: 5.09747E-11 score: 53.2247
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 105..158

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg21320	Carg21320	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg21320-RA:1	Carg21320-RA:1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg21320-RA:cds	Carg21320-RA:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg21320-RA	Carg21320-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0008270	zinc ion binding