Carg19325-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg19325-RA vs. NCBI nr
Match: KAG6581484.1 (Berberine bridge enzyme-like 15, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034774.1 Berberine bridge enzyme-like 15, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1120.9 bits (2898), Expect = 0.0e+00
Identity = 553/553 (100.00%), Postives = 553/553 (100.00%), Query Frame = 0

Query: 1   MRVLFINPKYKSFVSSLFLVFLLSSSTWVASASLQDSFLQCLNENSQFSVPYSTFCAPNN 60
           MRVLFINPKYKSFVSSLFLVFLLSSSTWVASASLQDSFLQCLNENSQFSVPYSTFCAPNN
Sbjct: 1   MRVLFINPKYKSFVSSLFLVFLLSSSTWVASASLQDSFLQCLNENSQFSVPYSTFCAPNN 60

Query: 61  ATFNTLLQSTAQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYE 120
           ATFNTLLQSTAQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYE
Sbjct: 61  ATFNTLLQSTAQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYE 120

Query: 121 GLSYVSQIESPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGL 180
           GLSYVSQIESPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGL
Sbjct: 121 GLSYVSQIESPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGL 180

Query: 181 CTSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGG 240
           CTSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGG
Sbjct: 181 CTSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGG 240

Query: 241 GSFGIILWWKLNLVPVPSIVTVFTVAKTLEQDATKILYKWQQVADKLDEDLFIRVIINVA 300
           GSFGIILWWKLNLVPVPSIVTVFTVAKTLEQDATKILYKWQQVADKLDEDLFIRVIINVA
Sbjct: 241 GSFGIILWWKLNLVPVPSIVTVFTVAKTLEQDATKILYKWQQVADKLDEDLFIRVIINVA 300

Query: 301 ANTDKQGQKTVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYP 360
           ANTDKQGQKTVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYP
Sbjct: 301 ANTDKQGQKTVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYP 360

Query: 361 SGTPPEVLLQGKSTFKNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMA 420
           SGTPPEVLLQGKSTFKNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMA
Sbjct: 361 SGTPPEVLLQGKSTFKNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMA 420

Query: 421 KISETQIPFPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVN 480
           KISETQIPFPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVN
Sbjct: 421 KISETQIPFPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVN 480

Query: 481 YRDLDLGINEKNSNTSYIEAIGWGTRYFKQNFGRLLRVKTKVDPDNFFRHEQSIPPIPTS 540
           YRDLDLGINEKNSNTSYIEAIGWGTRYFKQNFGRLLRVKTKVDPDNFFRHEQSIPPIPTS
Sbjct: 481 YRDLDLGINEKNSNTSYIEAIGWGTRYFKQNFGRLLRVKTKVDPDNFFRHEQSIPPIPTS 540

Query: 541 ESLRFGSSERGFD 554
           ESLRFGSSERGFD
Sbjct: 541 ESLRFGSSERGFD 553

BLAST of Carg19325-RA vs. NCBI nr
Match: XP_022925679.1 (berberine bridge enzyme-like 15 [Cucurbita moschata])

HSP 1 Score: 1119.4 bits (2894), Expect = 0.0e+00
Identity = 552/553 (99.82%), Postives = 552/553 (99.82%), Query Frame = 0

Query: 1   MRVLFINPKYKSFVSSLFLVFLLSSSTWVASASLQDSFLQCLNENSQFSVPYSTFCAPNN 60
           MRVLFINPKYKSFVSSLFLVFLLSSSTWVASASLQDSFLQCLNENSQFSVPYSTFCAPNN
Sbjct: 1   MRVLFINPKYKSFVSSLFLVFLLSSSTWVASASLQDSFLQCLNENSQFSVPYSTFCAPNN 60

Query: 61  ATFNTLLQSTAQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYE 120
           ATFNTLLQSTAQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYE
Sbjct: 61  ATFNTLLQSTAQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYE 120

Query: 121 GLSYVSQIESPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGL 180
           GLSYVSQIESPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRI EKSKVHGFPAGL
Sbjct: 121 GLSYVSQIESPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRITEKSKVHGFPAGL 180

Query: 181 CTSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGG 240
           CTSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGG
Sbjct: 181 CTSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGG 240

Query: 241 GSFGIILWWKLNLVPVPSIVTVFTVAKTLEQDATKILYKWQQVADKLDEDLFIRVIINVA 300
           GSFGIILWWKLNLVPVPSIVTVFTVAKTLEQDATKILYKWQQVADKLDEDLFIRVIINVA
Sbjct: 241 GSFGIILWWKLNLVPVPSIVTVFTVAKTLEQDATKILYKWQQVADKLDEDLFIRVIINVA 300

Query: 301 ANTDKQGQKTVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYP 360
           ANTDKQGQKTVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYP
Sbjct: 301 ANTDKQGQKTVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYP 360

Query: 361 SGTPPEVLLQGKSTFKNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMA 420
           SGTPPEVLLQGKSTFKNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMA
Sbjct: 361 SGTPPEVLLQGKSTFKNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMA 420

Query: 421 KISETQIPFPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVN 480
           KISETQIPFPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVN
Sbjct: 421 KISETQIPFPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVN 480

Query: 481 YRDLDLGINEKNSNTSYIEAIGWGTRYFKQNFGRLLRVKTKVDPDNFFRHEQSIPPIPTS 540
           YRDLDLGINEKNSNTSYIEAIGWGTRYFKQNFGRLLRVKTKVDPDNFFRHEQSIPPIPTS
Sbjct: 481 YRDLDLGINEKNSNTSYIEAIGWGTRYFKQNFGRLLRVKTKVDPDNFFRHEQSIPPIPTS 540

Query: 541 ESLRFGSSERGFD 554
           ESLRFGSSERGFD
Sbjct: 541 ESLRFGSSERGFD 553

BLAST of Carg19325-RA vs. NCBI nr
Match: XP_022977473.1 (berberine bridge enzyme-like 15 [Cucurbita maxima])

HSP 1 Score: 1115.1 bits (2883), Expect = 0.0e+00
Identity = 549/553 (99.28%), Postives = 553/553 (100.00%), Query Frame = 0

Query: 1   MRVLFINPKYKSFVSSLFLVFLLSSSTWVASASLQDSFLQCLNENSQFSVPYSTFCAPNN 60
           MRVLFINPKYKSFVSSLFLVFLLSSSTWVASASLQ+SFLQCLNENSQFSVPYSTFCAPNN
Sbjct: 1   MRVLFINPKYKSFVSSLFLVFLLSSSTWVASASLQESFLQCLNENSQFSVPYSTFCAPNN 60

Query: 61  ATFNTLLQSTAQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYE 120
           ATFNTLLQS+AQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYE
Sbjct: 61  ATFNTLLQSSAQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYE 120

Query: 121 GLSYVSQIESPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGL 180
           GLSYVSQIESPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGL
Sbjct: 121 GLSYVSQIESPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGL 180

Query: 181 CTSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGG 240
           CTSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGG
Sbjct: 181 CTSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGG 240

Query: 241 GSFGIILWWKLNLVPVPSIVTVFTVAKTLEQDATKILYKWQQVADKLDEDLFIRVIINVA 300
           GSFGIILWWKLNLVPVPSIVTVFTVAKTLEQDATKILYKWQQVADKLDEDLFIRVIINVA
Sbjct: 241 GSFGIILWWKLNLVPVPSIVTVFTVAKTLEQDATKILYKWQQVADKLDEDLFIRVIINVA 300

Query: 301 ANTDKQGQKTVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYP 360
           ANTDK+GQKTVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYP
Sbjct: 301 ANTDKKGQKTVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYP 360

Query: 361 SGTPPEVLLQGKSTFKNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMA 420
           SGTPPEVLLQGKSTFKNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMA
Sbjct: 361 SGTPPEVLLQGKSTFKNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMA 420

Query: 421 KISETQIPFPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVN 480
           KISETQIPFPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVN
Sbjct: 421 KISETQIPFPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVN 480

Query: 481 YRDLDLGINEKNSNTSYIEAIGWGTRYFKQNFGRLLRVKTKVDPDNFFRHEQSIPPIPTS 540
           YRDLDLGINEKNSNTSYIEAIGWGTRYF+QNFGRLLRVKTKVDPDNFFRHEQSIPPIPTS
Sbjct: 481 YRDLDLGINEKNSNTSYIEAIGWGTRYFRQNFGRLLRVKTKVDPDNFFRHEQSIPPIPTS 540

Query: 541 ESLRFGSSERGFD 554
           ESLRFGSSERGFD
Sbjct: 541 ESLRFGSSERGFD 553

BLAST of Carg19325-RA vs. NCBI nr
Match: XP_023544580.1 (berberine bridge enzyme-like 15 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1107.4 bits (2863), Expect = 0.0e+00
Identity = 548/553 (99.10%), Postives = 550/553 (99.46%), Query Frame = 0

Query: 1   MRVLFINPKYKSFVSSLFLVFLLSSSTWVASASLQDSFLQCLNENSQFSVPYSTFCAPNN 60
           MRVLFINPKYKSFVSSLFLVFLLSSSTWVASASLQ+SFLQCLNENSQFSVPYSTFCAPNN
Sbjct: 1   MRVLFINPKYKSFVSSLFLVFLLSSSTWVASASLQESFLQCLNENSQFSVPYSTFCAPNN 60

Query: 61  ATFNTLLQSTAQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYE 120
           ATFNTLLQSTAQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYE
Sbjct: 61  ATFNTLLQSTAQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYE 120

Query: 121 GLSYVSQIESPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGL 180
           GLSYVSQIESPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGL
Sbjct: 121 GLSYVSQIESPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGL 180

Query: 181 CTSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGG 240
           CTSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGG
Sbjct: 181 CTSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGG 240

Query: 241 GSFGIILWWKLNLVPVPSIVTVFTVAKTLEQDATKILYKWQQVADKLDEDLFIRVIINVA 300
           GSFGIILWWKLNLVPVPSIVTVFTVAKTLEQD TKILYKWQQVADKLDEDLFIRVIINVA
Sbjct: 241 GSFGIILWWKLNLVPVPSIVTVFTVAKTLEQDGTKILYKWQQVADKLDEDLFIRVIINVA 300

Query: 301 ANTDKQGQKTVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYP 360
           ANTDKQGQKTVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYP
Sbjct: 301 ANTDKQGQKTVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYP 360

Query: 361 SGTPPEVLLQGKSTFKNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMA 420
           SGTPPEVLLQGKSTFKNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMA
Sbjct: 361 SGTPPEVLLQGKSTFKNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMA 420

Query: 421 KISETQIPFPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVN 480
           KISETQIPFPHRRGVL+KIQYLTTWQK EGNEDRHLQWIKKLYNYMAPYVSQLPREAYVN
Sbjct: 421 KISETQIPFPHRRGVLFKIQYLTTWQK-EGNEDRHLQWIKKLYNYMAPYVSQLPREAYVN 480

Query: 481 YRDLDLGINEKNSNTSYIEAIGWGTRYFKQNFGRLLRVKTKVDPDNFFRHEQSIPPIPTS 540
           YRDLDLGINEKNSNTSYIEAIGWGTRYFKQNFGRLLRVKTKVDPDNFFRHEQSIPPIPTS
Sbjct: 481 YRDLDLGINEKNSNTSYIEAIGWGTRYFKQNFGRLLRVKTKVDPDNFFRHEQSIPPIPTS 540

Query: 541 ESLRFGSSERGFD 554
           ESLRFG SERGFD
Sbjct: 541 ESLRFGYSERGFD 552

BLAST of Carg19325-RA vs. NCBI nr
Match: XP_038882764.1 (berberine bridge enzyme-like 15 [Benincasa hispida])

HSP 1 Score: 1038.9 bits (2685), Expect = 1.6e-299
Identity = 508/552 (92.03%), Postives = 535/552 (96.92%), Query Frame = 0

Query: 3   VLFINPKYKSFVSSLFLVFLLSSSTWVASASLQDSFLQCLNENSQFSVPYSTFCAPNNAT 62
           ++F NPKYKSFV+SLFLVFLLSSS+WV SASL++SFLQCLNENSQFSVPYS+FCAPNNAT
Sbjct: 2   MVFTNPKYKSFVASLFLVFLLSSSSWVDSASLEESFLQCLNENSQFSVPYSSFCAPNNAT 61

Query: 63  FNTLLQSTAQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYEGL 122
           FN LLQSTAQNLRYLE SVPKPLFIFTPL DS+VQ+AVICSKQLKIHLRVRSGGHDYEGL
Sbjct: 62  FNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLKIHLRVRSGGHDYEGL 121

Query: 123 SYVSQIESPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCT 182
           SYVS+IE+PFIILDLAKLRSVKVDID NSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCT
Sbjct: 122 SYVSEIETPFIILDLAKLRSVKVDIDGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCT 181

Query: 183 SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGGGS 242
           SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRIL+REAMGEDLFWAIRGGGGGS
Sbjct: 182 SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGS 241

Query: 243 FGIILWWKLNLVPVPSIVTVFTVAKTLEQDATKILYKWQQVADKLDEDLFIRVIINVAAN 302
           FGIILWWKLNLVPVPS VTVFTVAKTLEQ ATKILYKWQQVADKLDEDLFIRVIINVAAN
Sbjct: 242 FGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN 301

Query: 303 TDKQGQKTVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYPSG 362
            DK+GQKTVSTAYNA FLG+A+RLLK+MGESFPELGLTRKDCIETSWIKSVLYIAGYPSG
Sbjct: 302 ADKKGQKTVSTAYNALFLGDASRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSG 361

Query: 363 TPPEVLLQGKSTFKNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMAKI 422
           TPPEVLLQGKSTFKN+FKAKSDFV+DPIPETGLEGLWKRL+E+ESPLMIWNPYGGMM KI
Sbjct: 362 TPPEVLLQGKSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKI 421

Query: 423 SETQIPFPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVNYR 482
           SET+IPFPHRRGVL+KIQYLTTWQK E N+D+HLQWIKKLYNYM PYVSQLPRE YVNYR
Sbjct: 422 SETEIPFPHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPRETYVNYR 481

Query: 483 DLDLGINEKNSNTSYIEAIGWGTRYFKQNFGRLLRVKTKVDPDNFFRHEQSIPPIPTSES 542
           DLDLGIN KNSNTSYIE+IGWGTRYFK+NFGRLLRVKTKVDPDNFFRHEQSIPPIPTSES
Sbjct: 482 DLDLGIN-KNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSES 541

Query: 543 LR-FGSSERGFD 554
           LR FGSS+RGFD
Sbjct: 542 LRSFGSSKRGFD 552

BLAST of Carg19325-RA vs. ExPASy Swiss-Prot
Match: O64743 (Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV=1)

HSP 1 Score: 736.5 bits (1900), Expect = 2.2e-211
Identity = 349/524 (66.60%), Postives = 437/524 (83.40%), Query Frame = 0

Query: 15  SSLFLVFLLSSSTWVASASLQDSFLQCLNENSQFSVPY--STFCAPNNAT-FNTLLQSTA 74
           ++LFLV LL  S  ++S++LQ  F++CL +NS  S P   S F    NAT F   L+STA
Sbjct: 10  ATLFLVTLLLISVPLSSSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELESTA 69

Query: 75  QNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYEGLSYVSQIESP 134
           QNLRYL  S PKP+FIF PL +++VQAAV+C+K+L++HLR+RSGGHDYEGLS+V++ E+P
Sbjct: 70  QNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETP 129

Query: 135 FIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVGGHIT 194
           F+I+DL+KLR V VD+DSNSAW  AGATIGEVYYRI EKS+ HGFPAGLC+SLG+GGH+ 
Sbjct: 130 FVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLV 189

Query: 195 GGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGGGSFGIILWWKL 254
           GGAYGSMMRK+GLGADNV+DARIVDANG+ILDR AMGED+FWAIRGGGGGSFG+IL WK+
Sbjct: 190 GGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKI 249

Query: 255 NLVPVPSIVTVFTVAKTLEQDATKILYKWQQVADKLDEDLFIRVIINVAANTDKQGQKTV 314
            LVPVP+ VTVFTV KTLEQD TK+LYKW+Q+ADKLD+DLFIRVII+ A+ T K G +T+
Sbjct: 250 KLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNRTI 309

Query: 315 STAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYPSGTPPEVLLQG 374
           S +Y AQFLG++NRLL++M +SFPELGLT+KDC E SWIKSV+YIAG+P+   PE LL G
Sbjct: 310 SMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEALLAG 369

Query: 375 KSTFKNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMAKISETQIPFPH 434
           KS FKN FKAKSDFV++PIP  GLEGLW+R  EE+SPL IWNPYGGMM++ISE++IPFPH
Sbjct: 370 KSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLTIWNPYGGMMSRISESEIPFPH 429

Query: 435 RRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVNYRDLDLGINEK 494
           R G L+KIQ+L+TWQ  + +E+RH++WI+++Y+YM  YVS+ PR+AYVNYRDLDLG NE 
Sbjct: 430 RNGTLFKIQWLSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTNEG 489

Query: 495 NSNTSYIEAIGWGTRYFKQNFGRLLRVKTKVDPDNFFRHEQSIP 536
            +     +A  WG +Y+K NF RL+++K + DPDNFFRHEQS+P
Sbjct: 490 ET-----DAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVP 528

BLAST of Carg19325-RA vs. ExPASy Swiss-Prot
Match: Q93ZA3 (Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=1 SV=1)

HSP 1 Score: 723.4 bits (1866), Expect = 1.9e-207
Identity = 349/527 (66.22%), Postives = 430/527 (81.59%), Query Frame = 0

Query: 15  SSLFLVFLLSSSTW---VASASLQDSFLQCLNENSQFSVPY-STFCAP--NNATFNTLLQ 74
           ++ FLV LL  S     ++ +++Q  F+ CL +NS  S P  S+F     N ++F   L+
Sbjct: 9   TNAFLVTLLLLSLSYIPLSFSTIQQDFVMCLVDNSDASFPMDSSFFTHDLNASSFKLALE 68

Query: 75  STAQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYEGLSYVSQI 134
           ++AQNLRYL  S PKP FIF PL +++VQAAV+C+K+LK+HLR+RSGGHDYEGLSYVS++
Sbjct: 69  TSAQNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEM 128

Query: 135 ESPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVGG 194
           E+ F+I+DL+KLR + VDI+SNSAWV AGA+IGEVYYRI EKSK+HGFPAGLCTSLG+GG
Sbjct: 129 ETAFVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGG 188

Query: 195 HITGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGGGSFGIILW 254
           HI GGAYGSMMRK+GLGADNV+DARIVDA+G+IL+R AMGED+FWAIRGGGGGSFG+IL 
Sbjct: 189 HIIGGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILA 248

Query: 255 WKLNLVPVPSIVTVFTVAKTLEQDATKILYKWQQVADKLDEDLFIRVIINVAANTDKQGQ 314
           WK+ LVPVP IVTVFTV +TLEQD TK+LYKWQQVADKLDEDLFIRVII   + T K  +
Sbjct: 249 WKIKLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPKSKE 308

Query: 315 KTVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYPSGTPPEVL 374
           +T+ST+Y  QFLG+ANRLL++M  SFP+LGLT+KDC+ETSWIKSV+YIAG+PS  P E L
Sbjct: 309 RTISTSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEAL 368

Query: 375 LQGKSTFKNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMAKISETQIP 434
           L GKS FKN+FKAKSD+V +PIP  GLEGLW++L EE+SPL IWNPYGGMMAKI ET+ P
Sbjct: 369 LDGKSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLTIWNPYGGMMAKIPETETP 428

Query: 435 FPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVNYRDLDLGI 494
           FPHR G L+KIQ+LT WQ  + +E +H+ W++++Y+YM  YVS+ PR AYVNYRDLDLG+
Sbjct: 429 FPHRSGTLFKIQWLTLWQDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGM 488

Query: 495 NEKNSNTSYIEAIGWGTRYFKQNFGRLLRVKTKVDPDNFFRHEQSIP 536
           N K S     +A  WG RYFK NF RL+ +K K DP+NFFRHEQSIP
Sbjct: 489 NGKGS-----DAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIP 530

BLAST of Carg19325-RA vs. ExPASy Swiss-Prot
Match: Q9FKU8 (Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=2 SV=1)

HSP 1 Score: 711.1 bits (1834), Expect = 1.0e-203
Identity = 348/529 (65.78%), Postives = 426/529 (80.53%), Query Frame = 0

Query: 15  SSLFLVFLLSSSTWVAS-ASLQDSFLQCLNENSQFSVPY-STFCAP--NNATFNTLLQST 74
           S L L F L + T  +S ASLQD F+ C+  N+    P   TF AP  N + F+ +L+ST
Sbjct: 11  SILVLYFSLYTITPTSSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLEST 70

Query: 75  AQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYEGLSYVSQIES 134
           AQNLR+L+ S+PKP FIF+P+ +S+VQA++ICSK+L++HLRVRSGGHDYEGLSYVSQI+ 
Sbjct: 71  AQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDK 130

Query: 135 PFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVGGHI 194
           PFI++DL+K+R V ++I  NSAWVQ+GAT+GE+YYRIAEKSKVHGFPAGLC+SLG+GGHI
Sbjct: 131 PFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHI 190

Query: 195 TGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGGGSFGIILWWK 254
           TGGAYGSMMRKYGLGADNV+DA+IVDANG++LDR AMGED FWAIRGG GGSFGIIL WK
Sbjct: 191 TGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWK 250

Query: 255 LNLVPVPSIVTVFTVAKTLEQD-ATKILYKWQQVADKLDEDLFIRVIINVAANTDKQGQK 314
           + LVPVP  VTVFTV KTL+QD   KI+ KWQ+VADKL E+LFIRV+ NVA      G K
Sbjct: 251 IKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGT---GGNK 310

Query: 315 TVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYPSGTPPEVLL 374
           TV+T+YNA FLG    L+ +M +SFPELGLT KDCIE SW++S+ YI+G+P+ TP  VLL
Sbjct: 311 TVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNVLL 370

Query: 375 QGKSTF-KNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMAKISETQIP 434
           QGKS F K  FKAKSDFV+ PIPE+GL+G++K+L +E+ PLMIWNPYGGMMAKI E+QIP
Sbjct: 371 QGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPLMIWNPYGGMMAKIPESQIP 430

Query: 435 FPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVNYRDLDLGI 494
           FPHR+GVL+K+QY+T+W   +    RH+ WI+ LY+YM PYVS  PREAYVNYRDLDLG 
Sbjct: 431 FPHRKGVLFKVQYVTSWLDSDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGR 490

Query: 495 NEKNSNTSYIEAIGWGTRYFKQNFGRLLRVKTKVDPDNFFRHEQSIPPI 538
           N K+  T   +A  WG  YFK NF RL+ +K KVDP+NFFRHEQSIPP+
Sbjct: 491 NTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPPM 536

BLAST of Carg19325-RA vs. ExPASy Swiss-Prot
Match: Q9FKU9 (Berberine bridge enzyme-like 25 OS=Arabidopsis thaliana OX=3702 GN=At5g44390 PE=2 SV=1)

HSP 1 Score: 662.9 bits (1709), Expect = 3.1e-189
Identity = 324/534 (60.67%), Postives = 408/534 (76.40%), Query Frame = 0

Query: 12  SFVSSLFLVFLLSSSTWVASASLQDSFLQCL--NENSQFSVPYSTFCAPNNAT-FNTLLQ 71
           S +S   L F   + T  +S SLQD F++CL  N N +F++  + F    NA+ F  +L+
Sbjct: 11  SCISVFALYFSFYTITLTSSTSLQDDFIKCLYRNTNVRFTLDKTFFTPERNASIFTEVLE 70

Query: 72  STAQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYEGLSYVSQI 131
           STAQN RYL  ++PKP FIF P+ +S+VQA+VICSK+L+IH RVRSGGHDYEG+SYVSQI
Sbjct: 71  STAQNQRYLTKTMPKPGFIFKPVHESHVQASVICSKKLEIHFRVRSGGHDYEGVSYVSQI 130

Query: 132 ESPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVGG 191
           E PF+++DL+KLR + VDI   SAWV+AGAT+GE+YYRIAEKSK HGFPAG+  SLG+GG
Sbjct: 131 EKPFVLIDLSKLRQINVDIKDTSAWVEAGATVGELYYRIAEKSKFHGFPAGVYPSLGIGG 190

Query: 192 HITGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGGGSFGIILW 251
           HITGGAYGS+MRKYGL ADNV+DA+IVDANG++LDR +MGEDLFWAIRGG GGSFGIIL 
Sbjct: 191 HITGGAYGSLMRKYGLAADNVLDAKIVDANGKLLDRASMGEDLFWAIRGGSGGSFGIILS 250

Query: 252 WKLNLVPVPSIVTVFTVAKTLEQDAT-KILYKWQQVADKLDEDLFIRVIINVAANTDKQG 311
           WK+ LVPVP  +TVFTV KT EQD + KIL KWQ++AD L ++LF+RV   V+ N   + 
Sbjct: 251 WKIKLVPVPETLTVFTVTKTFEQDRSFKILSKWQEIADNLVDELFLRVFFTVSGN---KA 310

Query: 312 QKTVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYPSGTPP-- 371
            KTV+ AY  QFLG    L+++M + FPELGLT+KDCIE SWI S++Y +G+P+  PP  
Sbjct: 311 NKTVTMAYIGQFLGEKGTLMEVMKKDFPELGLTQKDCIEMSWIDSIIYNSGFPTNPPPPI 370

Query: 372 EVLLQGKSTF-KNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMAKISE 431
           E+LLQ KS   K +FK KSDF + PIP  GLEG++K+L EE++ L+IW PYGG M KI E
Sbjct: 371 EILLQAKSPIGKVYFKGKSDFAKKPIPVLGLEGMFKKLLEEDAALVIWTPYGGKMDKIPE 430

Query: 432 TQIPFPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVNYRDL 491
           ++IPFPHR G  + IQY  +W   E   +R  +WI++LY YM PYVS  PR+AYVNYRDL
Sbjct: 431 SEIPFPHRNGTNFMIQYYRSWSDSEKRPNRRTKWIRELYGYMTPYVSSNPRQAYVNYRDL 490

Query: 492 DLGINEKNSNTSYIEAIGWGTRYFKQNFGRLLRVKTKVDPDNFFRHEQSIPPIP 539
           DLG N+ NS +++IEA  WG  YFK NF RL+R+K+KVDPDNFFRHEQSIP +P
Sbjct: 491 DLGQNKDNSKSNFIEAKIWGANYFKDNFNRLVRIKSKVDPDNFFRHEQSIPTLP 541

BLAST of Carg19325-RA vs. ExPASy Swiss-Prot
Match: Q9FKV0 (Berberine bridge enzyme-like 24 OS=Arabidopsis thaliana OX=3702 GN=At5g44380 PE=2 SV=1)

HSP 1 Score: 661.4 bits (1705), Expect = 9.1e-189
Identity = 317/530 (59.81%), Postives = 405/530 (76.42%), Query Frame = 0

Query: 14  VSSLFLVFLLSSSTWVASASLQDSFLQCLNENSQFSVPYSTFC---APNNATFNTLLQST 73
           VS L+  F   + T  +SAS+QD F+ C+  N+  S P        A N + FN +L+ST
Sbjct: 15  VSVLYFSFYCITPT-SSSASIQDQFINCVKRNTHVSFPLEKTLFTPAKNVSLFNQVLEST 74

Query: 74  AQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYEGLSYVSQIES 133
           AQNL++L  S+PKP FIF P+  S VQA++ICSK+L IH RVRSGGHD+E LSYVS+IE 
Sbjct: 75  AQNLQFLAKSMPKPGFIFRPIHQSQVQASIICSKKLGIHFRVRSGGHDFEALSYVSRIEK 134

Query: 134 PFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVGGHI 193
           PFI+LDL+KL+ + VDI+SNSAWVQ GAT+GE+YYRIAEKSK+HGFPAGLCTS+G+GG++
Sbjct: 135 PFILLDLSKLKQINVDIESNSAWVQPGATLGELYYRIAEKSKIHGFPAGLCTSVGIGGYM 194

Query: 194 TGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGGGSFGIILWWK 253
           TGG YG++MRKYGL  DNV+D ++VDANG++LDR AMGEDLFWAIRGGGG SFGI+L WK
Sbjct: 195 TGGGYGTLMRKYGLAGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGGASFGIVLAWK 254

Query: 254 LNLVPVPSIVTVFTVAKTLEQDA-TKILYKWQQVADKLDEDLFIRVIINVAANTDKQGQK 313
           + LVPVP  VTVFTV KTLEQDA  K + KWQQ++ K+ E++ IRV++  A N    G K
Sbjct: 255 IKLVPVPKTVTVFTVTKTLEQDARLKTISKWQQISSKIIEEIHIRVVLRAAGN---DGNK 314

Query: 314 TVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYPSGTPPEVLL 373
           TV+  Y  QFLG    LLK+M ++FPELGLT+KDC E SWI++ L+  G+P+G+P E+LL
Sbjct: 315 TVTMTYLGQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHGGFPTGSPIEILL 374

Query: 374 QGKSTF-KNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMAKISETQIP 433
           Q KS   K++FKA SDFV++PIP  GL+G++KRL E  +  + W PYGGMM+KI E+ IP
Sbjct: 375 QLKSPLGKDYFKATSDFVKEPIPVIGLKGIFKRLIEGNTTFLNWTPYGGMMSKIPESAIP 434

Query: 434 FPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVNYRDLDLGI 493
           FPHR G L+KI Y   W + +    R + WIK++YNYMAPYVS  PR+AYVNYRDLD G 
Sbjct: 435 FPHRNGTLFKILYYANWLENDKTSSRKINWIKEIYNYMAPYVSSNPRQAYVNYRDLDFGQ 494

Query: 494 NEKNSNTSYIEAIGWGTRYFKQNFGRLLRVKTKVDPDNFFRHEQSIPPIP 539
           N+ N+  ++IEA  WG +YFK NF RL+++KTKVDP+NFFRHEQSIPP+P
Sbjct: 495 NKNNAKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIPPMP 540

BLAST of Carg19325-RA vs. ExPASy TrEMBL
Match: A0A6J1ECW5 (berberine bridge enzyme-like 15 OS=Cucurbita moschata OX=3662 GN=LOC111433029 PE=3 SV=1)

HSP 1 Score: 1119.4 bits (2894), Expect = 0.0e+00
Identity = 552/553 (99.82%), Postives = 552/553 (99.82%), Query Frame = 0

Query: 1   MRVLFINPKYKSFVSSLFLVFLLSSSTWVASASLQDSFLQCLNENSQFSVPYSTFCAPNN 60
           MRVLFINPKYKSFVSSLFLVFLLSSSTWVASASLQDSFLQCLNENSQFSVPYSTFCAPNN
Sbjct: 1   MRVLFINPKYKSFVSSLFLVFLLSSSTWVASASLQDSFLQCLNENSQFSVPYSTFCAPNN 60

Query: 61  ATFNTLLQSTAQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYE 120
           ATFNTLLQSTAQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYE
Sbjct: 61  ATFNTLLQSTAQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYE 120

Query: 121 GLSYVSQIESPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGL 180
           GLSYVSQIESPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRI EKSKVHGFPAGL
Sbjct: 121 GLSYVSQIESPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRITEKSKVHGFPAGL 180

Query: 181 CTSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGG 240
           CTSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGG
Sbjct: 181 CTSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGG 240

Query: 241 GSFGIILWWKLNLVPVPSIVTVFTVAKTLEQDATKILYKWQQVADKLDEDLFIRVIINVA 300
           GSFGIILWWKLNLVPVPSIVTVFTVAKTLEQDATKILYKWQQVADKLDEDLFIRVIINVA
Sbjct: 241 GSFGIILWWKLNLVPVPSIVTVFTVAKTLEQDATKILYKWQQVADKLDEDLFIRVIINVA 300

Query: 301 ANTDKQGQKTVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYP 360
           ANTDKQGQKTVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYP
Sbjct: 301 ANTDKQGQKTVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYP 360

Query: 361 SGTPPEVLLQGKSTFKNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMA 420
           SGTPPEVLLQGKSTFKNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMA
Sbjct: 361 SGTPPEVLLQGKSTFKNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMA 420

Query: 421 KISETQIPFPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVN 480
           KISETQIPFPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVN
Sbjct: 421 KISETQIPFPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVN 480

Query: 481 YRDLDLGINEKNSNTSYIEAIGWGTRYFKQNFGRLLRVKTKVDPDNFFRHEQSIPPIPTS 540
           YRDLDLGINEKNSNTSYIEAIGWGTRYFKQNFGRLLRVKTKVDPDNFFRHEQSIPPIPTS
Sbjct: 481 YRDLDLGINEKNSNTSYIEAIGWGTRYFKQNFGRLLRVKTKVDPDNFFRHEQSIPPIPTS 540

Query: 541 ESLRFGSSERGFD 554
           ESLRFGSSERGFD
Sbjct: 541 ESLRFGSSERGFD 553

BLAST of Carg19325-RA vs. ExPASy TrEMBL
Match: A0A6J1IQ34 (berberine bridge enzyme-like 15 OS=Cucurbita maxima OX=3661 GN=LOC111477806 PE=3 SV=1)

HSP 1 Score: 1115.1 bits (2883), Expect = 0.0e+00
Identity = 549/553 (99.28%), Postives = 553/553 (100.00%), Query Frame = 0

Query: 1   MRVLFINPKYKSFVSSLFLVFLLSSSTWVASASLQDSFLQCLNENSQFSVPYSTFCAPNN 60
           MRVLFINPKYKSFVSSLFLVFLLSSSTWVASASLQ+SFLQCLNENSQFSVPYSTFCAPNN
Sbjct: 1   MRVLFINPKYKSFVSSLFLVFLLSSSTWVASASLQESFLQCLNENSQFSVPYSTFCAPNN 60

Query: 61  ATFNTLLQSTAQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYE 120
           ATFNTLLQS+AQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYE
Sbjct: 61  ATFNTLLQSSAQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYE 120

Query: 121 GLSYVSQIESPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGL 180
           GLSYVSQIESPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGL
Sbjct: 121 GLSYVSQIESPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGL 180

Query: 181 CTSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGG 240
           CTSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGG
Sbjct: 181 CTSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGG 240

Query: 241 GSFGIILWWKLNLVPVPSIVTVFTVAKTLEQDATKILYKWQQVADKLDEDLFIRVIINVA 300
           GSFGIILWWKLNLVPVPSIVTVFTVAKTLEQDATKILYKWQQVADKLDEDLFIRVIINVA
Sbjct: 241 GSFGIILWWKLNLVPVPSIVTVFTVAKTLEQDATKILYKWQQVADKLDEDLFIRVIINVA 300

Query: 301 ANTDKQGQKTVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYP 360
           ANTDK+GQKTVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYP
Sbjct: 301 ANTDKKGQKTVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYP 360

Query: 361 SGTPPEVLLQGKSTFKNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMA 420
           SGTPPEVLLQGKSTFKNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMA
Sbjct: 361 SGTPPEVLLQGKSTFKNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMA 420

Query: 421 KISETQIPFPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVN 480
           KISETQIPFPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVN
Sbjct: 421 KISETQIPFPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVN 480

Query: 481 YRDLDLGINEKNSNTSYIEAIGWGTRYFKQNFGRLLRVKTKVDPDNFFRHEQSIPPIPTS 540
           YRDLDLGINEKNSNTSYIEAIGWGTRYF+QNFGRLLRVKTKVDPDNFFRHEQSIPPIPTS
Sbjct: 481 YRDLDLGINEKNSNTSYIEAIGWGTRYFRQNFGRLLRVKTKVDPDNFFRHEQSIPPIPTS 540

Query: 541 ESLRFGSSERGFD 554
           ESLRFGSSERGFD
Sbjct: 541 ESLRFGSSERGFD 553

BLAST of Carg19325-RA vs. ExPASy TrEMBL
Match: A0A5A7UG10 (Flavin-dependent oxidoreductase FOX2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G009760 PE=3 SV=1)

HSP 1 Score: 1016.5 bits (2627), Expect = 4.1e-293
Identity = 497/548 (90.69%), Postives = 528/548 (96.35%), Query Frame = 0

Query: 7   NPKYKSFVSSLFLVFLLSSSTWVASASLQDSFLQCLNENSQFSVPYSTFCAPNNATFNTL 66
           NPKYKSFV+ LFLVFLLSSS+WV S+SL++SFLQCLNENSQFSVPYS+FCAPNN+TFN L
Sbjct: 6   NPKYKSFVAPLFLVFLLSSSSWVDSSSLEESFLQCLNENSQFSVPYSSFCAPNNSTFNAL 65

Query: 67  LQSTAQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYEGLSYVS 126
           LQSTAQNLRYLE SVPKPLFIFTPL DS+VQ+AVICSKQL+IHLRVRSGGHDYEGLSY S
Sbjct: 66  LQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLRIHLRVRSGGHDYEGLSYAS 125

Query: 127 QIESPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGV 186
           ++E+PFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGV
Sbjct: 126 EMETPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGV 185

Query: 187 GGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGGGSFGII 246
           GGHITGGAYGSMMRKYGLGADNVVDARIVDANGRIL+REAMGEDLFWAIRGGGGGSFGII
Sbjct: 186 GGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGSFGII 245

Query: 247 LWWKLNLVPVPSIVTVFTVAKTLEQDATKILYKWQQVADKLDEDLFIRVIINVAANTDKQ 306
           LWWKLNLVPVPS VTVFTVAKTLEQ ATKILYKWQQVADKLDEDLFIRVIINVAAN    
Sbjct: 246 LWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN---G 305

Query: 307 GQKTVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYPSGTPPE 366
           GQKTVSTAYNA FLG+A+RLLK+MGE+FPELGLTRKDCIETSWIKSVLYIAGYPSGTPPE
Sbjct: 306 GQKTVSTAYNALFLGDASRLLKVMGENFPELGLTRKDCIETSWIKSVLYIAGYPSGTPPE 365

Query: 367 VLLQGKSTFKNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMAKISETQ 426
           VLLQGKSTFKN+FKAKSDFV+ PIPETGLEGLWKRL+E+ESPLMIWNPYGGMM KISET+
Sbjct: 366 VLLQGKSTFKNYFKAKSDFVKTPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKISETE 425

Query: 427 IPFPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVNYRDLDL 486
           IPFPHRRGVL+KIQYLTTWQK E N+D+HLQWIKKLYNYM PYVSQLPREAYVNYRDLDL
Sbjct: 426 IPFPHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPREAYVNYRDLDL 485

Query: 487 GINEKNSNTSYIEAIGWGTRYFKQNFGRLLRVKTKVDPDNFFRHEQSIPPIPTSESLR-F 546
           GIN KNSNTSYIE+IGWGTRYFK+NFGRLLRVKTKVDPDNFFRHEQS+PPIPTSE+LR F
Sbjct: 486 GIN-KNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSVPPIPTSEALRSF 545

Query: 547 GSSERGFD 554
           GSS+RGFD
Sbjct: 546 GSSKRGFD 549

BLAST of Carg19325-RA vs. ExPASy TrEMBL
Match: A0A1S3AYY2 (flavin-dependent oxidoreductase FOX2-like OS=Cucumis melo OX=3656 GN=LOC103484298 PE=3 SV=1)

HSP 1 Score: 1015.8 bits (2625), Expect = 7.0e-293
Identity = 498/548 (90.88%), Postives = 527/548 (96.17%), Query Frame = 0

Query: 7   NPKYKSFVSSLFLVFLLSSSTWVASASLQDSFLQCLNENSQFSVPYSTFCAPNNATFNTL 66
           NPKYKSFV+ LFLVFLLSSS+WV S SL++SFLQCLNENSQFSVPYS+FCAPNN+TFN L
Sbjct: 6   NPKYKSFVAPLFLVFLLSSSSWVDSFSLEESFLQCLNENSQFSVPYSSFCAPNNSTFNAL 65

Query: 67  LQSTAQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYEGLSYVS 126
           LQSTAQNLRYLE SVPKPLFIFTPL DS+VQ+AVICSKQL+IHLRVRSGGHDYEGLSY S
Sbjct: 66  LQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLRIHLRVRSGGHDYEGLSYAS 125

Query: 127 QIESPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGV 186
           ++E+PFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGV
Sbjct: 126 EMETPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGV 185

Query: 187 GGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGGGSFGII 246
           GGHITGGAYGSMMRKYGLGADNVVDARIVDANGRIL+REAMGEDLFWAIRGGGGGSFGII
Sbjct: 186 GGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGSFGII 245

Query: 247 LWWKLNLVPVPSIVTVFTVAKTLEQDATKILYKWQQVADKLDEDLFIRVIINVAANTDKQ 306
           LWWKLNLVPVPS VTVFTVAKTLEQ ATKILYKWQQVADKLDEDLFIRVIINVAAN    
Sbjct: 246 LWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN---G 305

Query: 307 GQKTVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYPSGTPPE 366
           GQKTVSTAYNA FLG+A+RLLK+MGE+FPELGLTRKDCIETSWIKSVLYIAGYPSGTPPE
Sbjct: 306 GQKTVSTAYNALFLGDASRLLKVMGENFPELGLTRKDCIETSWIKSVLYIAGYPSGTPPE 365

Query: 367 VLLQGKSTFKNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMAKISETQ 426
           VLLQGKSTFKN+FKAKSDFV+ PIPETGLEGLWKRL+E+ESPLMIWNPYGGMM KISET+
Sbjct: 366 VLLQGKSTFKNYFKAKSDFVKTPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKISETE 425

Query: 427 IPFPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVNYRDLDL 486
           IPFPHRRGVL+KIQYLTTWQK E N+D+HLQWIKKLYNYM PYVSQLPREAYVNYRDLDL
Sbjct: 426 IPFPHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPREAYVNYRDLDL 485

Query: 487 GINEKNSNTSYIEAIGWGTRYFKQNFGRLLRVKTKVDPDNFFRHEQSIPPIPTSESLR-F 546
           GIN KNSNTSYIE+IGWGTRYFK+NFGRLLRVKTKVDPDNFFRHEQSIPPIPTSE+LR F
Sbjct: 486 GIN-KNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSEALRSF 545

Query: 547 GSSERGFD 554
           GSS+RGFD
Sbjct: 546 GSSKRGFD 549

BLAST of Carg19325-RA vs. ExPASy TrEMBL
Match: A0A6J1CYZ1 (berberine bridge enzyme-like 15 OS=Momordica charantia OX=3673 GN=LOC111015758 PE=3 SV=1)

HSP 1 Score: 986.5 bits (2549), Expect = 4.6e-284
Identity = 483/547 (88.30%), Postives = 513/547 (93.78%), Query Frame = 0

Query: 8   PKYKSFVSSLFLVFLLSSSTWVASASLQDSFLQCLNENSQFSVPYSTFCAPNNATFNTLL 67
           PKYKS V SLFLVFLLSSS WV SASL++SFLQCLNENSQFSVP+S+FCAPNNATFN LL
Sbjct: 4   PKYKSVVGSLFLVFLLSSSCWVDSASLEESFLQCLNENSQFSVPFSSFCAPNNATFNALL 63

Query: 68  QSTAQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYEGLSYVSQ 127
           QSTAQNLRYLE SVPKPLFIFTP  DS+VQAAVICSKQLKIHLRVRSGGHDYEGLSY S+
Sbjct: 64  QSTAQNLRYLETSVPKPLFIFTPAYDSHVQAAVICSKQLKIHLRVRSGGHDYEGLSYASE 123

Query: 128 IESPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVG 187
            E+ F+ILDLAKLRS+KVDI+ NSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVG
Sbjct: 124 TETSFMILDLAKLRSIKVDIEGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVG 183

Query: 188 GHITGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGGGSFGIIL 247
           GHITGGAYG MMRKYGLGADNVVDARIVDANGRILDREAMGED+FWAIRGGGGGSFGIIL
Sbjct: 184 GHITGGAYGPMMRKYGLGADNVVDARIVDANGRILDREAMGEDVFWAIRGGGGGSFGIIL 243

Query: 248 WWKLNLVPVPSIVTVFTVAKTLEQDATKILYKWQQVADKLDEDLFIRVIINVAANTDKQG 307
           WWKLNLVPVPS VTVFTVAKTLEQ ATKILYKWQQVADKLDEDLFIRVIINVAAN DK  
Sbjct: 244 WWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAANADK-- 303

Query: 308 QKTVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYPSGTPPEV 367
           +KTVSTAYNA FLG+ANRLLK+MGESFPELGLTRKDCIE SWIKSVLYIAG+PSGTPPEV
Sbjct: 304 KKTVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIEMSWIKSVLYIAGFPSGTPPEV 363

Query: 368 LLQGKSTFKNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMAKISETQI 427
           LLQGKSTFKN+FKAKSDFV++PI ETGLEGLWKRL+EEESPL IWNPYGGMM KISET+I
Sbjct: 364 LLQGKSTFKNYFKAKSDFVKEPISETGLEGLWKRLFEEESPLTIWNPYGGMMGKISETEI 423

Query: 428 PFPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVNYRDLDLG 487
           PFPHRRG+L+KIQYLT WQK + N+++HL+WIKKLYNYM PYVSQLPREAYVNYRDLDLG
Sbjct: 424 PFPHRRGILFKIQYLTLWQKGDSNQEKHLEWIKKLYNYMTPYVSQLPREAYVNYRDLDLG 483

Query: 488 INEKNSNTSYIEAIGWGTRYFKQNFGRLLRVKTKVDPDNFFRHEQSIPPIPTSESLR-FG 547
           IN+K  NTSYI+AIGWGTRYFK+NFGRLLRVKTKVDPDNFFRHEQSIPPIPTS S R FG
Sbjct: 484 INKK-GNTSYIDAIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSASFRSFG 543

Query: 548 SSERGFD 554
           SS R FD
Sbjct: 544 SSSREFD 547

BLAST of Carg19325-RA vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 736.5 bits (1900), Expect = 1.6e-212
Identity = 349/524 (66.60%), Postives = 437/524 (83.40%), Query Frame = 0

Query: 15  SSLFLVFLLSSSTWVASASLQDSFLQCLNENSQFSVPY--STFCAPNNAT-FNTLLQSTA 74
           ++LFLV LL  S  ++S++LQ  F++CL +NS  S P   S F    NAT F   L+STA
Sbjct: 10  ATLFLVTLLLISVPLSSSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELESTA 69

Query: 75  QNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYEGLSYVSQIESP 134
           QNLRYL  S PKP+FIF PL +++VQAAV+C+K+L++HLR+RSGGHDYEGLS+V++ E+P
Sbjct: 70  QNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETP 129

Query: 135 FIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVGGHIT 194
           F+I+DL+KLR V VD+DSNSAW  AGATIGEVYYRI EKS+ HGFPAGLC+SLG+GGH+ 
Sbjct: 130 FVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLV 189

Query: 195 GGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGGGSFGIILWWKL 254
           GGAYGSMMRK+GLGADNV+DARIVDANG+ILDR AMGED+FWAIRGGGGGSFG+IL WK+
Sbjct: 190 GGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKI 249

Query: 255 NLVPVPSIVTVFTVAKTLEQDATKILYKWQQVADKLDEDLFIRVIINVAANTDKQGQKTV 314
            LVPVP+ VTVFTV KTLEQD TK+LYKW+Q+ADKLD+DLFIRVII+ A+ T K G +T+
Sbjct: 250 KLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNRTI 309

Query: 315 STAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYPSGTPPEVLLQG 374
           S +Y AQFLG++NRLL++M +SFPELGLT+KDC E SWIKSV+YIAG+P+   PE LL G
Sbjct: 310 SMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEALLAG 369

Query: 375 KSTFKNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMAKISETQIPFPH 434
           KS FKN FKAKSDFV++PIP  GLEGLW+R  EE+SPL IWNPYGGMM++ISE++IPFPH
Sbjct: 370 KSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLTIWNPYGGMMSRISESEIPFPH 429

Query: 435 RRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVNYRDLDLGINEK 494
           R G L+KIQ+L+TWQ  + +E+RH++WI+++Y+YM  YVS+ PR+AYVNYRDLDLG NE 
Sbjct: 430 RNGTLFKIQWLSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTNEG 489

Query: 495 NSNTSYIEAIGWGTRYFKQNFGRLLRVKTKVDPDNFFRHEQSIP 536
            +     +A  WG +Y+K NF RL+++K + DPDNFFRHEQS+P
Sbjct: 490 ET-----DAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVP 528

BLAST of Carg19325-RA vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 723.4 bits (1866), Expect = 1.4e-208
Identity = 349/527 (66.22%), Postives = 430/527 (81.59%), Query Frame = 0

Query: 15  SSLFLVFLLSSSTW---VASASLQDSFLQCLNENSQFSVPY-STFCAP--NNATFNTLLQ 74
           ++ FLV LL  S     ++ +++Q  F+ CL +NS  S P  S+F     N ++F   L+
Sbjct: 9   TNAFLVTLLLLSLSYIPLSFSTIQQDFVMCLVDNSDASFPMDSSFFTHDLNASSFKLALE 68

Query: 75  STAQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYEGLSYVSQI 134
           ++AQNLRYL  S PKP FIF PL +++VQAAV+C+K+LK+HLR+RSGGHDYEGLSYVS++
Sbjct: 69  TSAQNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEM 128

Query: 135 ESPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVGG 194
           E+ F+I+DL+KLR + VDI+SNSAWV AGA+IGEVYYRI EKSK+HGFPAGLCTSLG+GG
Sbjct: 129 ETAFVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGG 188

Query: 195 HITGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGGGSFGIILW 254
           HI GGAYGSMMRK+GLGADNV+DARIVDA+G+IL+R AMGED+FWAIRGGGGGSFG+IL 
Sbjct: 189 HIIGGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILA 248

Query: 255 WKLNLVPVPSIVTVFTVAKTLEQDATKILYKWQQVADKLDEDLFIRVIINVAANTDKQGQ 314
           WK+ LVPVP IVTVFTV +TLEQD TK+LYKWQQVADKLDEDLFIRVII   + T K  +
Sbjct: 249 WKIKLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPKSKE 308

Query: 315 KTVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYPSGTPPEVL 374
           +T+ST+Y  QFLG+ANRLL++M  SFP+LGLT+KDC+ETSWIKSV+YIAG+PS  P E L
Sbjct: 309 RTISTSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEAL 368

Query: 375 LQGKSTFKNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMAKISETQIP 434
           L GKS FKN+FKAKSD+V +PIP  GLEGLW++L EE+SPL IWNPYGGMMAKI ET+ P
Sbjct: 369 LDGKSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLTIWNPYGGMMAKIPETETP 428

Query: 435 FPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVNYRDLDLGI 494
           FPHR G L+KIQ+LT WQ  + +E +H+ W++++Y+YM  YVS+ PR AYVNYRDLDLG+
Sbjct: 429 FPHRSGTLFKIQWLTLWQDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGM 488

Query: 495 NEKNSNTSYIEAIGWGTRYFKQNFGRLLRVKTKVDPDNFFRHEQSIP 536
           N K S     +A  WG RYFK NF RL+ +K K DP+NFFRHEQSIP
Sbjct: 489 NGKGS-----DAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIP 530

BLAST of Carg19325-RA vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 711.1 bits (1834), Expect = 7.1e-205
Identity = 348/529 (65.78%), Postives = 426/529 (80.53%), Query Frame = 0

Query: 15  SSLFLVFLLSSSTWVAS-ASLQDSFLQCLNENSQFSVPY-STFCAP--NNATFNTLLQST 74
           S L L F L + T  +S ASLQD F+ C+  N+    P   TF AP  N + F+ +L+ST
Sbjct: 11  SILVLYFSLYTITPTSSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLEST 70

Query: 75  AQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYEGLSYVSQIES 134
           AQNLR+L+ S+PKP FIF+P+ +S+VQA++ICSK+L++HLRVRSGGHDYEGLSYVSQI+ 
Sbjct: 71  AQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDK 130

Query: 135 PFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVGGHI 194
           PFI++DL+K+R V ++I  NSAWVQ+GAT+GE+YYRIAEKSKVHGFPAGLC+SLG+GGHI
Sbjct: 131 PFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHI 190

Query: 195 TGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGGGSFGIILWWK 254
           TGGAYGSMMRKYGLGADNV+DA+IVDANG++LDR AMGED FWAIRGG GGSFGIIL WK
Sbjct: 191 TGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWK 250

Query: 255 LNLVPVPSIVTVFTVAKTLEQD-ATKILYKWQQVADKLDEDLFIRVIINVAANTDKQGQK 314
           + LVPVP  VTVFTV KTL+QD   KI+ KWQ+VADKL E+LFIRV+ NVA      G K
Sbjct: 251 IKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGT---GGNK 310

Query: 315 TVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYPSGTPPEVLL 374
           TV+T+YNA FLG    L+ +M +SFPELGLT KDCIE SW++S+ YI+G+P+ TP  VLL
Sbjct: 311 TVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNVLL 370

Query: 375 QGKSTF-KNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMAKISETQIP 434
           QGKS F K  FKAKSDFV+ PIPE+GL+G++K+L +E+ PLMIWNPYGGMMAKI E+QIP
Sbjct: 371 QGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPLMIWNPYGGMMAKIPESQIP 430

Query: 435 FPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVNYRDLDLGI 494
           FPHR+GVL+K+QY+T+W   +    RH+ WI+ LY+YM PYVS  PREAYVNYRDLDLG 
Sbjct: 431 FPHRKGVLFKVQYVTSWLDSDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGR 490

Query: 495 NEKNSNTSYIEAIGWGTRYFKQNFGRLLRVKTKVDPDNFFRHEQSIPPI 538
           N K+  T   +A  WG  YFK NF RL+ +K KVDP+NFFRHEQSIPP+
Sbjct: 491 NTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPPM 536

BLAST of Carg19325-RA vs. TAIR 10
Match: AT5G44390.1 (FAD-binding Berberine family protein )

HSP 1 Score: 662.9 bits (1709), Expect = 2.2e-190
Identity = 324/534 (60.67%), Postives = 408/534 (76.40%), Query Frame = 0

Query: 12  SFVSSLFLVFLLSSSTWVASASLQDSFLQCL--NENSQFSVPYSTFCAPNNAT-FNTLLQ 71
           S +S   L F   + T  +S SLQD F++CL  N N +F++  + F    NA+ F  +L+
Sbjct: 11  SCISVFALYFSFYTITLTSSTSLQDDFIKCLYRNTNVRFTLDKTFFTPERNASIFTEVLE 70

Query: 72  STAQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYEGLSYVSQI 131
           STAQN RYL  ++PKP FIF P+ +S+VQA+VICSK+L+IH RVRSGGHDYEG+SYVSQI
Sbjct: 71  STAQNQRYLTKTMPKPGFIFKPVHESHVQASVICSKKLEIHFRVRSGGHDYEGVSYVSQI 130

Query: 132 ESPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVGG 191
           E PF+++DL+KLR + VDI   SAWV+AGAT+GE+YYRIAEKSK HGFPAG+  SLG+GG
Sbjct: 131 EKPFVLIDLSKLRQINVDIKDTSAWVEAGATVGELYYRIAEKSKFHGFPAGVYPSLGIGG 190

Query: 192 HITGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGGGSFGIILW 251
           HITGGAYGS+MRKYGL ADNV+DA+IVDANG++LDR +MGEDLFWAIRGG GGSFGIIL 
Sbjct: 191 HITGGAYGSLMRKYGLAADNVLDAKIVDANGKLLDRASMGEDLFWAIRGGSGGSFGIILS 250

Query: 252 WKLNLVPVPSIVTVFTVAKTLEQDAT-KILYKWQQVADKLDEDLFIRVIINVAANTDKQG 311
           WK+ LVPVP  +TVFTV KT EQD + KIL KWQ++AD L ++LF+RV   V+ N   + 
Sbjct: 251 WKIKLVPVPETLTVFTVTKTFEQDRSFKILSKWQEIADNLVDELFLRVFFTVSGN---KA 310

Query: 312 QKTVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYPSGTPP-- 371
            KTV+ AY  QFLG    L+++M + FPELGLT+KDCIE SWI S++Y +G+P+  PP  
Sbjct: 311 NKTVTMAYIGQFLGEKGTLMEVMKKDFPELGLTQKDCIEMSWIDSIIYNSGFPTNPPPPI 370

Query: 372 EVLLQGKSTF-KNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMAKISE 431
           E+LLQ KS   K +FK KSDF + PIP  GLEG++K+L EE++ L+IW PYGG M KI E
Sbjct: 371 EILLQAKSPIGKVYFKGKSDFAKKPIPVLGLEGMFKKLLEEDAALVIWTPYGGKMDKIPE 430

Query: 432 TQIPFPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVNYRDL 491
           ++IPFPHR G  + IQY  +W   E   +R  +WI++LY YM PYVS  PR+AYVNYRDL
Sbjct: 431 SEIPFPHRNGTNFMIQYYRSWSDSEKRPNRRTKWIRELYGYMTPYVSSNPRQAYVNYRDL 490

Query: 492 DLGINEKNSNTSYIEAIGWGTRYFKQNFGRLLRVKTKVDPDNFFRHEQSIPPIP 539
           DLG N+ NS +++IEA  WG  YFK NF RL+R+K+KVDPDNFFRHEQSIP +P
Sbjct: 491 DLGQNKDNSKSNFIEAKIWGANYFKDNFNRLVRIKSKVDPDNFFRHEQSIPTLP 541

BLAST of Carg19325-RA vs. TAIR 10
Match: AT5G44380.1 (FAD-binding Berberine family protein )

HSP 1 Score: 661.4 bits (1705), Expect = 6.5e-190
Identity = 317/530 (59.81%), Postives = 405/530 (76.42%), Query Frame = 0

Query: 14  VSSLFLVFLLSSSTWVASASLQDSFLQCLNENSQFSVPYSTFC---APNNATFNTLLQST 73
           VS L+  F   + T  +SAS+QD F+ C+  N+  S P        A N + FN +L+ST
Sbjct: 15  VSVLYFSFYCITPT-SSSASIQDQFINCVKRNTHVSFPLEKTLFTPAKNVSLFNQVLEST 74

Query: 74  AQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYEGLSYVSQIES 133
           AQNL++L  S+PKP FIF P+  S VQA++ICSK+L IH RVRSGGHD+E LSYVS+IE 
Sbjct: 75  AQNLQFLAKSMPKPGFIFRPIHQSQVQASIICSKKLGIHFRVRSGGHDFEALSYVSRIEK 134

Query: 134 PFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVGGHI 193
           PFI+LDL+KL+ + VDI+SNSAWVQ GAT+GE+YYRIAEKSK+HGFPAGLCTS+G+GG++
Sbjct: 135 PFILLDLSKLKQINVDIESNSAWVQPGATLGELYYRIAEKSKIHGFPAGLCTSVGIGGYM 194

Query: 194 TGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGGGSFGIILWWK 253
           TGG YG++MRKYGL  DNV+D ++VDANG++LDR AMGEDLFWAIRGGGG SFGI+L WK
Sbjct: 195 TGGGYGTLMRKYGLAGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGGASFGIVLAWK 254

Query: 254 LNLVPVPSIVTVFTVAKTLEQDA-TKILYKWQQVADKLDEDLFIRVIINVAANTDKQGQK 313
           + LVPVP  VTVFTV KTLEQDA  K + KWQQ++ K+ E++ IRV++  A N    G K
Sbjct: 255 IKLVPVPKTVTVFTVTKTLEQDARLKTISKWQQISSKIIEEIHIRVVLRAAGN---DGNK 314

Query: 314 TVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYPSGTPPEVLL 373
           TV+  Y  QFLG    LLK+M ++FPELGLT+KDC E SWI++ L+  G+P+G+P E+LL
Sbjct: 315 TVTMTYLGQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHGGFPTGSPIEILL 374

Query: 374 QGKSTF-KNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMAKISETQIP 433
           Q KS   K++FKA SDFV++PIP  GL+G++KRL E  +  + W PYGGMM+KI E+ IP
Sbjct: 375 QLKSPLGKDYFKATSDFVKEPIPVIGLKGIFKRLIEGNTTFLNWTPYGGMMSKIPESAIP 434

Query: 434 FPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVNYRDLDLGI 493
           FPHR G L+KI Y   W + +    R + WIK++YNYMAPYVS  PR+AYVNYRDLD G 
Sbjct: 435 FPHRNGTLFKILYYANWLENDKTSSRKINWIKEIYNYMAPYVSSNPRQAYVNYRDLDFGQ 494

Query: 494 NEKNSNTSYIEAIGWGTRYFKQNFGRLLRVKTKVDPDNFFRHEQSIPPIP 539
           N+ N+  ++IEA  WG +YFK NF RL+++KTKVDP+NFFRHEQSIPP+P
Sbjct: 495 NKNNAKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIPPMP 540