Carg17843-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg17843-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	serine/arginine-rich SC35-like splicing factor SCL30A
Location	Carg_Chr10: 4376932 .. 4379500 (-)
Sequence length	486
RNA-Seq Expression	Carg17843-RA
Synteny	Carg17843-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCATGTGAGGTTTATCAAGTTAGAGTTTCTTGTTATGTGGTAGCTGAGTAGGTTGCTTACTGTTCTCGATTTCTGTACAAGGAAACAGCTTTGGATCAATTCAGTTGCCTTAAGAATTTGTAATTACATGTTAAAATAGTTTTGAGTGGTTCAATCTTGTTCACTTTCACAGCTGTAGCGAGATTCTTAGACTGCGGGTCTCTGTCATTGGTTGCTGTGCAAATGAGGGGTAGGAGTTATACTCCATCACCGCCAAGGGGTTATGGAAGAAGGGGTCGGAGCCCTAGCCCTAGAGGGCGCTATGGGGGACGTGGAAGAGACCTCCCCACTAGCCTTCTAGTTCGTAATCTTCGCCGTGACTGCAGGTTTGTTCTGGATGAGTATGTTATGTAGTTATGCTTATGTTTCTTATTGCCCCAAATGGTTTTTCGACCAATTTTCCTTCTCTAGGAGAAAGGGGGTCGCTTTATTTTATTTTGTTTTGAATTGATAGTGGTCAGATCTTTGTGCTAGAAAGGAGCTTAGATAGGGGAATGGGAATAAGACATCACATAGTTTAAGGAAGTTGAAAAGTTCTATCTGTTTCTGTAACCGCCCAAGCCCACTGCTAGCATATATTGTCCTCTTTGAGTTTTCCCTTTCGGGCATACCTCAAGGTTTTTAAAACTCGTCTGGGAGAGGTTTCCACACCCTTATAAAACACACTCTCTCTCAAAGTATTAGAATTCTACAATAATCATGAAATCTCTTATTCCAACAGCTATGAAAGAAACAAAATGAAATGGACCAAATAACTCTTTATAGTTCCTTTCATTGTCTTATAGGAGCCTTCTATTTATTTGTTGCCTACGAAGTGTCTTAAGGCCGATCCCTTCACTTGCGGCAAGGCACTCACCCCTCAATAAGGCTTGCTGTGTTGTGCCTTGGGTTAATTGACTTGTTTTTCCCGTCGTTTTATGCCCTCCTTTGGAAAGCTATTGTTATATCTTTCAATAATCTCGTGCAAATGCTCCCATATTTGTAAAAGTTTTTTTAAATGAAATTATGTTCATGATTCTGTTTGATTTTTGTATAAAGTCGTTGCAGCCAAGCGTGCCTCGCACTTTAAAAACATTGTTCCACCCTAATACATAGTATGTATTCCACCACATTAGGTAGCCTTTTATTTCCTTAGACAGAAGCCAAGTTATAGTAATTCATTTGATCTTCTTTCATTTTTCTTCTCCCTTGCGGTGCATCCGTGATTCAGTTTTGACCTGCATCCATGAAATGAGATGAGTCTTTCGTGTCTGAATATAAGAATTTAATTTGGTTGCATTCTTGCAGGGTCTTGTTCGGAATTGCAAGTGCTTTGTAGAACTCTGTGTAGTTTGAAATTAGTAATAGATTGATATAGCACCATTCGTGCTCTGGTTCCAATTTTTTTAACTGCATATTGTTTTCTTAAAACACATGTACTGCATACTGCTATTCTTTCTTGCTTGCTGATCAAATATATTCAAGATGTTGCTTAAAAAGCAATTTATAGTAAAAATATGATATAACCTGGTGTACCACTTATTAAAATTTATTTTTTGACACAGACCTGAGGATCTTCGTAGACCATTTGGACAGTTTGGTGCCATTAAGGACATATACCTACCAAAGGATTATCATACTGGGTGAGCATATCTGAGCTGTATGGTAGGATTTGCAGATTCTTGGGAAGTGTTAATTTTTTCAATTAGAAAAAAATACAATTAAACTTTGTGCATATGGTTTTTAAGTAATTCTGTAAATTTTATACAACGTTTTTAGTCTCTGTGATATAATATATTTAGTGGTATCGGAGAGAACATGTTTTCAATGATTTCCTTGCTTTCGAACAAAATGGTTTTAGCACAATCCTTGAGAAACGTTTTTAGTATCTATTGTGTTTCATCTTTCAAATTGTGCATCTGATGATAGAGGCATTTCTTTCTGGTTCTTTTGCTTTTTTGTTTTGTTTTTCCTTAAATCACATATTAGGTCTGTTTATTGAACGTTTGAAGATTTAACAGTGAAAAATGTTTTGAAGAAGACATCAAGTAATTAGAGTTCTATTGAAGATGATGTTAATGGTAGTGAAGATTGGAAGGAAGTTGGATTTGAGTACATAGAAGATGTTCCCAAGTCTTTGAAGAATTTCAAGTCATCTTAAATCTGACACATGCAAGGTCAGCCAGCCAAGTGCTTGCTTATATTCCAAGGGATATGGTGGACAGTTTTATTAGAGAAGGTTTCTAGCAGGCCAAGTTCCTGACCTGATTCTGTGGCTTTACATTAACTTTTCTTTTATAACTTTCCCCTTTTTGCTTCTTTTGTGCAACAATTTTGCAGAGAACCACGTGGCTTCGGTTTCGTTCAATATGTAGATCCTGCTGATGCTGCAGATGCTAAGCATCAGATGGATGGCTGTGTTCTTCTCGGTCGGGAGCTGACTGTGGTCTATGCTGAGGAAAACAGGAAGAAGCCATCTGATATGAGGACGAGGGAGAGAGGAAGGTATGAAGCTGGCCTTGTATCAATTGTTTACAGTTCAGTATAG

mRNA sequence

ATGCCATCTGTAGCGAGATTCTTAGACTGCGGGTCTCTGTCATTGGTTGCTGTGCAAATGAGGGGTAGGAGTTATACTCCATCACCGCCAAGGGGTTATGGAAGAAGGGGTCGGAGCCCTAGCCCTAGAGGGCGCTATGGGGGACGTGGAAGAGACCTCCCCACTAGCCTTCTAGTTCGTAATCTTCGCCGTGACTGCAGACCTGAGGATCTTCGTAGACCATTTGGACAGTTTGGTGCCATTAAGGACATATACCTACCAAAGGATTATCATACTGGAGAACCACGTGGCTTCGGTTTCGTTCAATATGTAGATCCTGCTGATGCTGCAGATGCTAAGCATCAGATGGATGGCTGTGTTCTTCTCGGTCGGGAGCTGACTGTGGTCTATGCTGAGGAAAACAGGAAGAAGCCATCTGATATGAGGACGAGGGAGAGAGGAAGGTATGAAGCTGGCCTTGTATCAATTGTTTACAGTTCAGTATAG

Coding sequence (CDS)

ATGCCATCTGTAGCGAGATTCTTAGACTGCGGGTCTCTGTCATTGGTTGCTGTGCAAATGAGGGGTAGGAGTTATACTCCATCACCGCCAAGGGGTTATGGAAGAAGGGGTCGGAGCCCTAGCCCTAGAGGGCGCTATGGGGGACGTGGAAGAGACCTCCCCACTAGCCTTCTAGTTCGTAATCTTCGCCGTGACTGCAGACCTGAGGATCTTCGTAGACCATTTGGACAGTTTGGTGCCATTAAGGACATATACCTACCAAAGGATTATCATACTGGAGAACCACGTGGCTTCGGTTTCGTTCAATATGTAGATCCTGCTGATGCTGCAGATGCTAAGCATCAGATGGATGGCTGTGTTCTTCTCGGTCGGGAGCTGACTGTGGTCTATGCTGAGGAAAACAGGAAGAAGCCATCTGATATGAGGACGAGGGAGAGAGGAAGGTATGAAGCTGGCCTTGTATCAATTGTTTACAGTTCAGTATAG

Protein sequence

MPSVARFLDCGSLSLVAVQMRGRSYTPSPPRGYGRRGRSPSPRGRYGGRGRDLPTSLLVRNLRRDCRPEDLRRPFGQFGAIKDIYLPKDYHTGEPRGFGFVQYVDPADAADAKHQMDGCVLLGRELTVVYAEENRKKPSDMRTRERGRYEAGLVSIVYSSV

Homology

[Close]

BLAST of Carg17843-RA vs. NCBI nr
Match: KAG7023812.1 (Serine/arginine-rich SC35-like splicing factor SCL30A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 333.6 bits (854), Expect = 9.6e-88
Identity = 161/161 (100.00%), Postives = 161/161 (100.00%), Query Frame = 0


Query: 1   MPSVARFLDCGSLSLVAVQMRGRSYTPSPPRGYGRRGRSPSPRGRYGGRGRDLPTSLLVR 60
           MPSVARFLDCGSLSLVAVQMRGRSYTPSPPRGYGRRGRSPSPRGRYGGRGRDLPTSLLVR
Sbjct: 1   MPSVARFLDCGSLSLVAVQMRGRSYTPSPPRGYGRRGRSPSPRGRYGGRGRDLPTSLLVR 60

Query: 61  NLRRDCRPEDLRRPFGQFGAIKDIYLPKDYHTGEPRGFGFVQYVDPADAADAKHQMDGCV 120
           NLRRDCRPEDLRRPFGQFGAIKDIYLPKDYHTGEPRGFGFVQYVDPADAADAKHQMDGCV
Sbjct: 61  NLRRDCRPEDLRRPFGQFGAIKDIYLPKDYHTGEPRGFGFVQYVDPADAADAKHQMDGCV 120

Query: 121 LLGRELTVVYAEENRKKPSDMRTRERGRYEAGLVSIVYSSV 162
           LLGRELTVVYAEENRKKPSDMRTRERGRYEAGLVSIVYSSV
Sbjct: 121 LLGRELTVVYAEENRKKPSDMRTRERGRYEAGLVSIVYSSV 161

[Close]

BLAST of Carg17843-RA vs. NCBI nr
Match: KAA0055935.1 (serine/arginine-rich SC35-like splicing factor SCL30A isoform X1 [Cucumis melo var. makuwa] >TYK28141.1 serine/arginine-rich SC35-like splicing factor SCL30A isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 279.6 bits (714), Expect = 1.7e-71
Identity = 135/145 (93.10%), Postives = 137/145 (94.48%), Query Frame = 0


Query: 3   SVARFLDCGSLSLVAVQMRGRSYTPSPPRGYGRRGRSPSPRGRYGGRGRDLPTSLLVRNL 62
           S ARFLD G LSLVAVQMRGRSYTPSPPRGYGRRGRSPSPRGRY GRGRDLPTSLLVRNL
Sbjct: 15  SPARFLDSGPLSLVAVQMRGRSYTPSPPRGYGRRGRSPSPRGRYVGRGRDLPTSLLVRNL 74

Query: 63  RRDCRPEDLRRPFGQFGAIKDIYLPKDYHTGEPRGFGFVQYVDPADAADAKHQMDGCVLL 122
             DCRPEDLRRPFGQFGAIKDIYLPKDY+TGEPRGFGFVQYVDPADAADAKH MDGCVLL
Sbjct: 75  SHDCRPEDLRRPFGQFGAIKDIYLPKDYYTGEPRGFGFVQYVDPADAADAKHHMDGCVLL 134

Query: 123 GRELTVVYAEENRKKPSDMRTRERG 148
           GRELTVV+AEENRKKPSDMR RERG
Sbjct: 135 GRELTVVFAEENRKKPSDMRARERG 159

[Close]

BLAST of Carg17843-RA vs. NCBI nr
Match: XP_022988197.1 (serine/arginine-rich SC35-like splicing factor SCL33 isoform X1 [Cucurbita maxima] >XP_022988198.1 serine/arginine-rich SC35-like splicing factor SCL33 isoform X1 [Cucurbita maxima])

HSP 1 Score: 271.6 bits (693), Expect = 4.5e-69
Identity = 128/130 (98.46%), Postives = 129/130 (99.23%), Query Frame = 0


Query: 20  MRGRSYTPSPPRGYGRRGRSPSPRGRYGGRGRDLPTSLLVRNLRRDCRPEDLRRPFGQFG 79
           MRGRSYTPSPPRGYGRRGRSPSPRGRYGGRGRDLPTSLLVRNLRRDCRPEDLRRPFGQFG
Sbjct: 1   MRGRSYTPSPPRGYGRRGRSPSPRGRYGGRGRDLPTSLLVRNLRRDCRPEDLRRPFGQFG 60

Query: 80  AIKDIYLPKDYHTGEPRGFGFVQYVDPADAADAKHQMDGCVLLGRELTVVYAEENRKKPS 139
           AIKDIYLPKDYHTGEPRGFGFVQYVDPADAADAKHQMDGCVLLGRELTVVYAEENRKKPS
Sbjct: 61  AIKDIYLPKDYHTGEPRGFGFVQYVDPADAADAKHQMDGCVLLGRELTVVYAEENRKKPS 120

Query: 140 DMRTRERGRY 150
           DMRTRERG +
Sbjct: 121 DMRTRERGSF 130

[Close]

BLAST of Carg17843-RA vs. NCBI nr
Match: XP_022961137.1 (serine/arginine-rich SC35-like splicing factor SCL33 isoform X2 [Cucurbita moschata] >KAG6590145.1 Serine/arginine-rich SC35-like splicing factor SCL30A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 271.2 bits (692), Expect = 5.9e-69
Identity = 128/128 (100.00%), Postives = 128/128 (100.00%), Query Frame = 0


Query: 20  MRGRSYTPSPPRGYGRRGRSPSPRGRYGGRGRDLPTSLLVRNLRRDCRPEDLRRPFGQFG 79
           MRGRSYTPSPPRGYGRRGRSPSPRGRYGGRGRDLPTSLLVRNLRRDCRPEDLRRPFGQFG
Sbjct: 1   MRGRSYTPSPPRGYGRRGRSPSPRGRYGGRGRDLPTSLLVRNLRRDCRPEDLRRPFGQFG 60

Query: 80  AIKDIYLPKDYHTGEPRGFGFVQYVDPADAADAKHQMDGCVLLGRELTVVYAEENRKKPS 139
           AIKDIYLPKDYHTGEPRGFGFVQYVDPADAADAKHQMDGCVLLGRELTVVYAEENRKKPS
Sbjct: 61  AIKDIYLPKDYHTGEPRGFGFVQYVDPADAADAKHQMDGCVLLGRELTVVYAEENRKKPS 120

Query: 140 DMRTRERG 148
           DMRTRERG
Sbjct: 121 DMRTRERG 128

[Close]

BLAST of Carg17843-RA vs. NCBI nr
Match: XP_022988199.1 (serine/arginine-rich SC35-like splicing factor SCL33 isoform X2 [Cucurbita maxima])

HSP 1 Score: 271.2 bits (692), Expect = 5.9e-69
Identity = 128/128 (100.00%), Postives = 128/128 (100.00%), Query Frame = 0


Query: 20  MRGRSYTPSPPRGYGRRGRSPSPRGRYGGRGRDLPTSLLVRNLRRDCRPEDLRRPFGQFG 79
           MRGRSYTPSPPRGYGRRGRSPSPRGRYGGRGRDLPTSLLVRNLRRDCRPEDLRRPFGQFG
Sbjct: 1   MRGRSYTPSPPRGYGRRGRSPSPRGRYGGRGRDLPTSLLVRNLRRDCRPEDLRRPFGQFG 60

Query: 80  AIKDIYLPKDYHTGEPRGFGFVQYVDPADAADAKHQMDGCVLLGRELTVVYAEENRKKPS 139
           AIKDIYLPKDYHTGEPRGFGFVQYVDPADAADAKHQMDGCVLLGRELTVVYAEENRKKPS
Sbjct: 61  AIKDIYLPKDYHTGEPRGFGFVQYVDPADAADAKHQMDGCVLLGRELTVVYAEENRKKPS 120

Query: 140 DMRTRERG 148
           DMRTRERG
Sbjct: 121 DMRTRERG 128

[Close]

BLAST of Carg17843-RA vs. ExPASy Swiss-Prot
Match: Q9SEU4 (Serine/arginine-rich SC35-like splicing factor SCL33 OS=Arabidopsis thaliana OX=3702 GN=SCL33 PE=1 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 1.8e-60
Identity = 108/128 (84.38%), Postives = 119/128 (92.97%), Query Frame = 0


Query: 20  MRGRSYTPSPPRGYGRRGRSPSPRGRYGGRGRDLPTSLLVRNLRRDCRPEDLRRPFGQFG 79
           MRGRSYTPSPPRGYGRRGRSPSPRGRYGGR RDLPTSLLVRNLR DCR EDLR+ F QFG
Sbjct: 1   MRGRSYTPSPPRGYGRRGRSPSPRGRYGGRSRDLPTSLLVRNLRHDCRQEDLRKSFEQFG 60

Query: 80  AIKDIYLPKDYHTGEPRGFGFVQYVDPADAADAKHQMDGCVLLGRELTVVYAEENRKKPS 139
            +KDIYLP+DY+TG+PRGFGFVQ++DPADAADAKH MDG +LLGRELTVV+AEENRKKP+
Sbjct: 61  PVKDIYLPRDYYTGDPRGFGFVQFMDPADAADAKHHMDGYLLLGRELTVVFAEENRKKPT 120

Query: 140 DMRTRERG 148
           +MR RERG
Sbjct: 121 EMRARERG 128

[Close]

BLAST of Carg17843-RA vs. ExPASy Swiss-Prot
Match: Q9LHP2 (Serine/arginine-rich SC35-like splicing factor SCL30A OS=Arabidopsis thaliana OX=3702 GN=SCL30A PE=1 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 2.6e-59
Identity = 107/129 (82.95%), Postives = 121/129 (93.80%), Query Frame = 0


Query: 20  MRGRSYTPSPPRGYGRRGRSPSPRGRYGG-RGRDLPTSLLVRNLRRDCRPEDLRRPFGQF 79
           MRGRSYTPSPPRGYGRRGRSPSPRGR+GG R  DLPTSLLVRNLR DCR EDLRRPF QF
Sbjct: 1   MRGRSYTPSPPRGYGRRGRSPSPRGRFGGSRDSDLPTSLLVRNLRHDCRQEDLRRPFEQF 60

Query: 80  GAIKDIYLPKDYHTGEPRGFGFVQYVDPADAADAKHQMDGCVLLGRELTVVYAEENRKKP 139
           G +KDIYLP+DY+TG+PRGFGF+Q++DPADAA+AKHQMDG +LLGRELTVV+AEENRKKP
Sbjct: 61  GPVKDIYLPRDYYTGDPRGFGFIQFMDPADAAEAKHQMDGYLLLGRELTVVFAEENRKKP 120

Query: 140 SDMRTRERG 148
           ++MRTR+RG
Sbjct: 121 TEMRTRDRG 129

[Close]

BLAST of Carg17843-RA vs. ExPASy Swiss-Prot
Match: Q8L3X8 (Serine/arginine-rich SC35-like splicing factor SCL30 OS=Arabidopsis thaliana OX=3702 GN=SCL30 PE=1 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 8.6e-31
Identity = 73/145 (50.34%), Postives = 95/145 (65.52%), Query Frame = 0


Query: 23  RSYTP---SPP-RGYGRRGRSPSP---------RGRYGGRGRDLPTSLLVRNLRRDCRPE 82
           R Y+P   SPP RGYG RGRSP P          G  GGR      SLLVRN+  DCRPE
Sbjct: 2   RRYSPPYYSPPRRGYGGRGRSPPPPPPRRGYGGGGGGGGRRGSSHGSLLVRNIPLDCRPE 61

Query: 83  DLRRPFGQFGAIKDIYLPKDYHTGEPRGFGFVQYVDPADAADAKHQMDGCVLLGRELTVV 142
           +LR PF +FG ++D+Y+P+DY++G+PRGF FV++VD  DA +A+  M+     GRE+TVV
Sbjct: 62  ELREPFERFGPVRDVYIPRDYYSGQPRGFAFVEFVDAYDAGEAQRSMNRRSFAGREITVV 121

Query: 143 YAEENRKKPSDMR--TRERGRYEAG 153
            A E+RK+P +MR  TR R R  +G
Sbjct: 122 VASESRKRPEEMRVKTRTRSREPSG 146

[Close]

BLAST of Carg17843-RA vs. ExPASy Swiss-Prot
Match: Q1PDV2 (Serine/arginine-rich SC35-like splicing factor SCL28 OS=Arabidopsis thaliana OX=3702 GN=SCL28 PE=1 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 7.6e-27
Identity = 70/142 (49.30%), Postives = 90/142 (63.38%), Query Frame = 0


Query: 15  LVAVQMRGRSYTPSPPRGYGRRGRSPSPRGRYG---GRGRDLPTS----------LLVRN 74
           +   + R RSY+P P      R RSP PR R G    R R+ P+S          LL+RN
Sbjct: 1   MARARSRSRSYSPRP------RDRSP-PRERKGYDDNRLRERPSSRDHESSGPSGLLIRN 60

Query: 75  LRRDCRPEDLRRPFGQFGAIKDIYLPKDYHTGEPRGFGFVQYVDPADAADAKHQMDGCVL 134
           L  D RP DLR  F +FG +KDIYLP++Y+TGEPRGFGFV+Y    DAA+A  +M+  V+
Sbjct: 61  LPLDARPNDLRDSFERFGPLKDIYLPRNYYTGEPRGFGFVKYRYAEDAAEAMKRMNHKVI 120

Query: 135 LGRELTVVYAEENRKKPSDMRT 144
            GRE+ +V+AEENRK P +MRT
Sbjct: 121 GGREIAIVFAEENRKTPQEMRT 135

[Close]

BLAST of Carg17843-RA vs. ExPASy Swiss-Prot
Match: O75494 (Serine/arginine-rich splicing factor 10 OS=Homo sapiens OX=9606 GN=SRSF10 PE=1 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 3.1e-20
Identity = 43/91 (47.25%), Postives = 63/91 (69.23%), Query Frame = 0


Query: 55  TSLLVRNLRRDCRPEDLRRPFGQFGAIKDIYLPKDYHTGEPRGFGFVQYVDPADAADAKH 114
           TSL VRN+  D R EDLRR FG++G I D+Y+P D++T  PRGF +VQ+ D  DA DA H
Sbjct: 10  TSLFVRNVADDTRSEDLRREFGRYGPIVDVYVPLDFYTRRPRGFAYVQFEDVRDAEDALH 69

Query: 115 QMDGCVLLGRELTVVYAEENRKKPSDMRTRE 146
            +D   + GR++ + +A+ +RK P+ M+ +E
Sbjct: 70  NLDRKWICGRQIEIQFAQGDRKTPNQMKAKE 100

[Close]

BLAST of Carg17843-RA vs. ExPASy TrEMBL
Match: A0A5A7UQN8 (Serine/arginine-rich SC35-like splicing factor SCL30A isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold289G00520 PE=4 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 8.0e-72
Identity = 135/145 (93.10%), Postives = 137/145 (94.48%), Query Frame = 0


Query: 3   SVARFLDCGSLSLVAVQMRGRSYTPSPPRGYGRRGRSPSPRGRYGGRGRDLPTSLLVRNL 62
           S ARFLD G LSLVAVQMRGRSYTPSPPRGYGRRGRSPSPRGRY GRGRDLPTSLLVRNL
Sbjct: 15  SPARFLDSGPLSLVAVQMRGRSYTPSPPRGYGRRGRSPSPRGRYVGRGRDLPTSLLVRNL 74

Query: 63  RRDCRPEDLRRPFGQFGAIKDIYLPKDYHTGEPRGFGFVQYVDPADAADAKHQMDGCVLL 122
             DCRPEDLRRPFGQFGAIKDIYLPKDY+TGEPRGFGFVQYVDPADAADAKH MDGCVLL
Sbjct: 75  SHDCRPEDLRRPFGQFGAIKDIYLPKDYYTGEPRGFGFVQYVDPADAADAKHHMDGCVLL 134

Query: 123 GRELTVVYAEENRKKPSDMRTRERG 148
           GRELTVV+AEENRKKPSDMR RERG
Sbjct: 135 GRELTVVFAEENRKKPSDMRARERG 159

[Close]

BLAST of Carg17843-RA vs. ExPASy TrEMBL
Match: A0A0A0LZX6 (RRM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G630290 PE=4 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 4.0e-71
Identity = 134/147 (91.16%), Postives = 137/147 (93.20%), Query Frame = 0


Query: 1   MPSVARFLDCGSLSLVAVQMRGRSYTPSPPRGYGRRGRSPSPRGRYGGRGRDLPTSLLVR 60
           + S ARFLD G LSLVAV MRGRSYTPSPPRGYGRRGRSPSPRGRY GRGRDLPTSLLVR
Sbjct: 100 LTSPARFLDSGPLSLVAVLMRGRSYTPSPPRGYGRRGRSPSPRGRYVGRGRDLPTSLLVR 159

Query: 61  NLRRDCRPEDLRRPFGQFGAIKDIYLPKDYHTGEPRGFGFVQYVDPADAADAKHQMDGCV 120
           NL  DCRPEDLRRPFGQFGAIKDIYLPKDY+TGEPRGFGFVQYVDPADAADAKH MDGCV
Sbjct: 160 NLSHDCRPEDLRRPFGQFGAIKDIYLPKDYYTGEPRGFGFVQYVDPADAADAKHHMDGCV 219

Query: 121 LLGRELTVVYAEENRKKPSDMRTRERG 148
           LLGRELTVV+AEENRKKPSDMR RERG
Sbjct: 220 LLGRELTVVFAEENRKKPSDMRARERG 246

[Close]

BLAST of Carg17843-RA vs. ExPASy TrEMBL
Match: A0A6J1JLK2 (serine/arginine-rich SC35-like splicing factor SCL33 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485508 PE=4 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 2.2e-69
Identity = 128/130 (98.46%), Postives = 129/130 (99.23%), Query Frame = 0


Query: 20  MRGRSYTPSPPRGYGRRGRSPSPRGRYGGRGRDLPTSLLVRNLRRDCRPEDLRRPFGQFG 79
           MRGRSYTPSPPRGYGRRGRSPSPRGRYGGRGRDLPTSLLVRNLRRDCRPEDLRRPFGQFG
Sbjct: 1   MRGRSYTPSPPRGYGRRGRSPSPRGRYGGRGRDLPTSLLVRNLRRDCRPEDLRRPFGQFG 60

Query: 80  AIKDIYLPKDYHTGEPRGFGFVQYVDPADAADAKHQMDGCVLLGRELTVVYAEENRKKPS 139
           AIKDIYLPKDYHTGEPRGFGFVQYVDPADAADAKHQMDGCVLLGRELTVVYAEENRKKPS
Sbjct: 61  AIKDIYLPKDYHTGEPRGFGFVQYVDPADAADAKHQMDGCVLLGRELTVVYAEENRKKPS 120

Query: 140 DMRTRERGRY 150
           DMRTRERG +
Sbjct: 121 DMRTRERGSF 130

[Close]

BLAST of Carg17843-RA vs. ExPASy TrEMBL
Match: A0A6J1HB72 (serine/arginine-rich SC35-like splicing factor SCL33 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111461738 PE=4 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 2.8e-69
Identity = 128/128 (100.00%), Postives = 128/128 (100.00%), Query Frame = 0


Query: 20  MRGRSYTPSPPRGYGRRGRSPSPRGRYGGRGRDLPTSLLVRNLRRDCRPEDLRRPFGQFG 79
           MRGRSYTPSPPRGYGRRGRSPSPRGRYGGRGRDLPTSLLVRNLRRDCRPEDLRRPFGQFG
Sbjct: 1   MRGRSYTPSPPRGYGRRGRSPSPRGRYGGRGRDLPTSLLVRNLRRDCRPEDLRRPFGQFG 60

Query: 80  AIKDIYLPKDYHTGEPRGFGFVQYVDPADAADAKHQMDGCVLLGRELTVVYAEENRKKPS 139
           AIKDIYLPKDYHTGEPRGFGFVQYVDPADAADAKHQMDGCVLLGRELTVVYAEENRKKPS
Sbjct: 61  AIKDIYLPKDYHTGEPRGFGFVQYVDPADAADAKHQMDGCVLLGRELTVVYAEENRKKPS 120

Query: 140 DMRTRERG 148
           DMRTRERG
Sbjct: 121 DMRTRERG 128

[Close]

BLAST of Carg17843-RA vs. ExPASy TrEMBL
Match: A0A6J1JKW9 (serine/arginine-rich SC35-like splicing factor SCL33 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485508 PE=4 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 2.8e-69
Identity = 128/128 (100.00%), Postives = 128/128 (100.00%), Query Frame = 0


Query: 20  MRGRSYTPSPPRGYGRRGRSPSPRGRYGGRGRDLPTSLLVRNLRRDCRPEDLRRPFGQFG 79
           MRGRSYTPSPPRGYGRRGRSPSPRGRYGGRGRDLPTSLLVRNLRRDCRPEDLRRPFGQFG
Sbjct: 1   MRGRSYTPSPPRGYGRRGRSPSPRGRYGGRGRDLPTSLLVRNLRRDCRPEDLRRPFGQFG 60

Query: 80  AIKDIYLPKDYHTGEPRGFGFVQYVDPADAADAKHQMDGCVLLGRELTVVYAEENRKKPS 139
           AIKDIYLPKDYHTGEPRGFGFVQYVDPADAADAKHQMDGCVLLGRELTVVYAEENRKKPS
Sbjct: 61  AIKDIYLPKDYHTGEPRGFGFVQYVDPADAADAKHQMDGCVLLGRELTVVYAEENRKKPS 120

Query: 140 DMRTRERG 148
           DMRTRERG
Sbjct: 121 DMRTRERG 128

[Close]

BLAST of Carg17843-RA vs. TAIR 10
Match: AT1G55310.1 (SC35-like splicing factor 33 )

HSP 1 Score: 233.4 bits (594), Expect = 1.3e-61
Identity = 108/128 (84.38%), Postives = 119/128 (92.97%), Query Frame = 0


Query: 20  MRGRSYTPSPPRGYGRRGRSPSPRGRYGGRGRDLPTSLLVRNLRRDCRPEDLRRPFGQFG 79
           MRGRSYTPSPPRGYGRRGRSPSPRGRYGGR RDLPTSLLVRNLR DCR EDLR+ F QFG
Sbjct: 1   MRGRSYTPSPPRGYGRRGRSPSPRGRYGGRSRDLPTSLLVRNLRHDCRQEDLRKSFEQFG 60

Query: 80  AIKDIYLPKDYHTGEPRGFGFVQYVDPADAADAKHQMDGCVLLGRELTVVYAEENRKKPS 139
            +KDIYLP+DY+TG+PRGFGFVQ++DPADAADAKH MDG +LLGRELTVV+AEENRKKP+
Sbjct: 61  PVKDIYLPRDYYTGDPRGFGFVQFMDPADAADAKHHMDGYLLLGRELTVVFAEENRKKPT 120

Query: 140 DMRTRERG 148
           +MR RERG
Sbjct: 121 EMRARERG 128

[Close]

BLAST of Carg17843-RA vs. TAIR 10
Match: AT1G55310.2 (SC35-like splicing factor 33 )

HSP 1 Score: 233.4 bits (594), Expect = 1.3e-61
Identity = 108/128 (84.38%), Postives = 119/128 (92.97%), Query Frame = 0


Query: 20  MRGRSYTPSPPRGYGRRGRSPSPRGRYGGRGRDLPTSLLVRNLRRDCRPEDLRRPFGQFG 79
           MRGRSYTPSPPRGYGRRGRSPSPRGRYGGR RDLPTSLLVRNLR DCR EDLR+ F QFG
Sbjct: 1   MRGRSYTPSPPRGYGRRGRSPSPRGRYGGRSRDLPTSLLVRNLRHDCRQEDLRKSFEQFG 60

Query: 80  AIKDIYLPKDYHTGEPRGFGFVQYVDPADAADAKHQMDGCVLLGRELTVVYAEENRKKPS 139
            +KDIYLP+DY+TG+PRGFGFVQ++DPADAADAKH MDG +LLGRELTVV+AEENRKKP+
Sbjct: 61  PVKDIYLPRDYYTGDPRGFGFVQFMDPADAADAKHHMDGYLLLGRELTVVFAEENRKKPT 120

Query: 140 DMRTRERG 148
           +MR RERG
Sbjct: 121 EMRARERG 128

[Close]

BLAST of Carg17843-RA vs. TAIR 10
Match: AT3G13570.1 (SC35-like splicing factor 30A )

HSP 1 Score: 229.6 bits (584), Expect = 1.8e-60
Identity = 107/129 (82.95%), Postives = 121/129 (93.80%), Query Frame = 0


Query: 20  MRGRSYTPSPPRGYGRRGRSPSPRGRYGG-RGRDLPTSLLVRNLRRDCRPEDLRRPFGQF 79
           MRGRSYTPSPPRGYGRRGRSPSPRGR+GG R  DLPTSLLVRNLR DCR EDLRRPF QF
Sbjct: 1   MRGRSYTPSPPRGYGRRGRSPSPRGRFGGSRDSDLPTSLLVRNLRHDCRQEDLRRPFEQF 60

Query: 80  GAIKDIYLPKDYHTGEPRGFGFVQYVDPADAADAKHQMDGCVLLGRELTVVYAEENRKKP 139
           G +KDIYLP+DY+TG+PRGFGF+Q++DPADAA+AKHQMDG +LLGRELTVV+AEENRKKP
Sbjct: 61  GPVKDIYLPRDYYTGDPRGFGFIQFMDPADAAEAKHQMDGYLLLGRELTVVFAEENRKKP 120

Query: 140 SDMRTRERG 148
           ++MRTR+RG
Sbjct: 121 TEMRTRDRG 129

[Close]

BLAST of Carg17843-RA vs. TAIR 10
Match: AT1G55310.3 (SC35-like splicing factor 33 )

HSP 1 Score: 224.6 bits (571), Expect = 5.9e-59
Identity = 108/140 (77.14%), Postives = 119/140 (85.00%), Query Frame = 0


Query: 20  MRGRSYTPSPPRGYGRRGRSPSPRGRYGGRGRDLPTSLLVRNLRRDCRPEDLRRPFGQFG 79
           MRGRSYTPSPPRGYGRRGRSPSPRGRYGGR RDLPTSLLVRNLR DCR EDLR+ F QFG
Sbjct: 1   MRGRSYTPSPPRGYGRRGRSPSPRGRYGGRSRDLPTSLLVRNLRHDCRQEDLRKSFEQFG 60

Query: 80  AIKDIYLPKDYHTG------------EPRGFGFVQYVDPADAADAKHQMDGCVLLGRELT 139
            +KDIYLP+DY+TG            +PRGFGFVQ++DPADAADAKH MDG +LLGRELT
Sbjct: 61  PVKDIYLPRDYYTGSHLCTDTCKASRDPRGFGFVQFMDPADAADAKHHMDGYLLLGRELT 120

Query: 140 VVYAEENRKKPSDMRTRERG 148
           VV+AEENRKKP++MR RERG
Sbjct: 121 VVFAEENRKKPTEMRARERG 140

[Close]

BLAST of Carg17843-RA vs. TAIR 10
Match: AT3G55460.1 (SC35-like splicing factor 30 )

HSP 1 Score: 134.8 bits (338), Expect = 6.1e-32
Identity = 73/145 (50.34%), Postives = 95/145 (65.52%), Query Frame = 0


Query: 23  RSYTP---SPP-RGYGRRGRSPSP---------RGRYGGRGRDLPTSLLVRNLRRDCRPE 82
           R Y+P   SPP RGYG RGRSP P          G  GGR      SLLVRN+  DCRPE
Sbjct: 2   RRYSPPYYSPPRRGYGGRGRSPPPPPPRRGYGGGGGGGGRRGSSHGSLLVRNIPLDCRPE 61

Query: 83  DLRRPFGQFGAIKDIYLPKDYHTGEPRGFGFVQYVDPADAADAKHQMDGCVLLGRELTVV 142
           +LR PF +FG ++D+Y+P+DY++G+PRGF FV++VD  DA +A+  M+     GRE+TVV
Sbjct: 62  ELREPFERFGPVRDVYIPRDYYSGQPRGFAFVEFVDAYDAGEAQRSMNRRSFAGREITVV 121

Query: 143 YAEENRKKPSDMR--TRERGRYEAG 153
            A E+RK+P +MR  TR R R  +G
Sbjct: 122 VASESRKRPEEMRVKTRTRSREPSG 146

The following BLAST results are available for this feature:

BLAST of Carg17843-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7023812.1	9.6e-88	100.00	Serine/arginine-rich SC35-like splicing factor SCL30A, partial [Cucurbita argyro...	[more]
KAA0055935.1	1.7e-71	93.10	serine/arginine-rich SC35-like splicing factor SCL30A isoform X1 [Cucumis melo v...	[more]
XP_022988197.1	4.5e-69	98.46	serine/arginine-rich SC35-like splicing factor SCL33 isoform X1 [Cucurbita maxim...	[more]
XP_022961137.1	5.9e-69	100.00	serine/arginine-rich SC35-like splicing factor SCL33 isoform X2 [Cucurbita mosch...	[more]
XP_022988199.1	5.9e-69	100.00	serine/arginine-rich SC35-like splicing factor SCL33 isoform X2 [Cucurbita maxim...	[more]

BLAST of Carg17843-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SEU4	1.8e-60	84.38	Serine/arginine-rich SC35-like splicing factor SCL33 OS=Arabidopsis thaliana OX=...	[more]
Q9LHP2	2.6e-59	82.95	Serine/arginine-rich SC35-like splicing factor SCL30A OS=Arabidopsis thaliana OX...	[more]
Q8L3X8	8.6e-31	50.34	Serine/arginine-rich SC35-like splicing factor SCL30 OS=Arabidopsis thaliana OX=...	[more]
Q1PDV2	7.6e-27	49.30	Serine/arginine-rich SC35-like splicing factor SCL28 OS=Arabidopsis thaliana OX=...	[more]
O75494	3.1e-20	47.25	Serine/arginine-rich splicing factor 10 OS=Homo sapiens OX=9606 GN=SRSF10 PE=1 S...	[more]

BLAST of Carg17843-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UQN8	8.0e-72	93.10	Serine/arginine-rich SC35-like splicing factor SCL30A isoform X1 OS=Cucumis melo...	[more]
A0A0A0LZX6	4.0e-71	91.16	RRM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G630290 PE=4 SV...	[more]
A0A6J1JLK2	2.2e-69	98.46	serine/arginine-rich SC35-like splicing factor SCL33 isoform X1 OS=Cucurbita max...	[more]
A0A6J1HB72	2.8e-69	100.00	serine/arginine-rich SC35-like splicing factor SCL33 isoform X1 OS=Cucurbita mos...	[more]
A0A6J1JKW9	2.8e-69	100.00	serine/arginine-rich SC35-like splicing factor SCL33 isoform X2 OS=Cucurbita max...	[more]

BLAST of Carg17843-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G55310.1	1.3e-61	84.38	SC35-like splicing factor 33	[more]
AT1G55310.2	1.3e-61	84.38	SC35-like splicing factor 33	[more]
AT3G13570.1	1.8e-60	82.95	SC35-like splicing factor 30A	[more]
AT1G55310.3	5.9e-59	77.14	SC35-like splicing factor 33	[more]
AT3G55460.1	6.1e-32	50.34	SC35-like splicing factor 30	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000504	RNA recognition motif domain	SMART	SM00360	rrm1_1	coord: 56..129 e-value: 4.4E-20 score: 82.7
IPR000504	RNA recognition motif domain	PFAM	PF00076	RRM_1	coord: 57..126 e-value: 4.8E-19 score: 68.0
IPR000504	RNA recognition motif domain	PROSITE	PS50102	RRM	coord: 55..133 score: 17.080015
IPR012677	Nucleotide-binding alpha-beta plait domain superfamily	GENE3D	3.30.70.330		coord: 51..154 e-value: 7.7E-28 score: 99.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 21..52
None	No IPR available	PANTHER	PTHR23147:SF149	SERINE/ARGININE-RICH SC35-LIKE SPLICING FACTOR SCL30A	coord: 20..150
None	No IPR available	PANTHER	PTHR23147	SERINE/ARGININE RICH SPLICING FACTOR	coord: 20..150
IPR035979	RNA-binding domain superfamily	SUPERFAMILY	54928	RNA-binding domain, RBD	coord: 36..144

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg17843	Carg17843	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg17843-RA:1	Carg17843-RA:1	exon
Carg17843-RA:2	Carg17843-RA:2	exon
Carg17843-RA:3	Carg17843-RA:3	exon
Carg17843-RA:4	Carg17843-RA:4	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg17843-RA:cds	Carg17843-RA:cds_4	CDS
Carg17843-RA:cds	Carg17843-RA:cds_3	CDS
Carg17843-RA:cds	Carg17843-RA:cds_2	CDS
Carg17843-RA:cds	Carg17843-RA:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg17843-RA	Carg17843-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0003723	RNA binding