Carg17713-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg17713-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Pectinesterase
Location	Carg_Chr04: 17173079 .. 17174418 (+)
Sequence length	1233
RNA-Seq Expression	Carg17713-RA
Synteny	Carg17713-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATCGGGAAGGTGGTCGTTTCGGGTGTATCCTTTATTCTCGTCGTAGGTGTAGCTCTTGCCGTCGTTGCAGTCGTTAACAAGAGAAATGACACGGCAGCTGAGATGCAGACTCTGTCCCCAAAGATGAAGGCAGTCGCAGGCATTTGTTCGGGTACTGATTATCCGAAGGAATGCCAATCTACTCTTTCCACAACTGCACAAAATTCCACTTCAGATGATCCTAAACAATATCTTAAAGCGGCCATTGTTGCCACTGTTGAAGAGATTACAAAAGGCTTGAATTTAACAGATACCCTTCGAGTGGAGGCTGCTGATAATACCACCATCCAAATGTCTGTTGATGATTGCAAGGACTTATTGCAGTCTGCTATTGACTCGCTTCATGCATCTCAGTCCACTGTTGGGGACCCTGATATGCACACTAATGCAGACCGTGTCGGTGATGTTAAGACATGGCTCAGCGCTGTCATTTCTTTTCAGCAATCTTGTCTCGATGGGCTGGAGGAAACTGATCCCGATCTCAGACAGAAGATGCAAGGCGAGCTTGATTCTGCTGGCAAGCTTACCAGCAATGCTCTTGCTATCCTCGATGAAGTTGTGGGGATATTGGAGAAGCTTGGCTTGCAATTGAAGGTTCAACCAAGTGGGCGTAGGCTTCTTGGGACGACAGAAGTAGGAAGCGATGGGTTCCCGACATGGTTGGTGGGGGAAGACAGGAAGCTGCTGGCAGCTAGAGGAGGCGCCGGGCGAGTGAAGCCCAACGCCGTGGTTGCTAAGGATGGTAGTGGGCAGTTCAAGACCATTGCCGCTGCATTGGCTGCATACCCAAAGGTTCCAACGGGAAGATATGTGATCTATGTGAAAGCAGGGGTGTATGATGAGTACATCATTGTGACTAAAAACATGGAGAACATTTTCATGTATGGAGATGGCCCTAGATCAACTATTGTCACTGGCCGTAAGAGCAACCGCGATGGCGTTACCACCCAAGACAGTGCCTCCTTCTGTAAGTAATTCGAGAAACCCTAAATCCCCAAATCGCTAAATCCCTAAAACCCTAAATCTCTAGATTGTTGAGAAACTAACAAATTGAACCGTAAAATTACCTCGCAGCGGCCATAGGAAATGGATTCCTCTGCAAATCCATGGGATTCAGCAACACAGCCGGCCCCGAAGGCCACCAGGCCGTAGCCCTAAGAGTCCAATCCGACAAGTCCGCGTTCTTCAACTGCAGAATGGACGGGCACCAAGACACCCTGTACGTTCAAGCCAACCGTCAATTCTACAGGAACTGCGTGATCTCTGGCACCGTGGACTTCATCTTCGGCGATTCGTAA

mRNA sequence

ATGATCGGGAAGGTGGTCGTTTCGGGTGTATCCTTTATTCTCGTCGTAGGTGTAGCTCTTGCCGTCGTTGCAGTCGTTAACAAGAGAAATGACACGGCAGCTGAGATGCAGACTCTGTCCCCAAAGATGAAGGCAGTCGCAGGCATTTGTTCGGGTACTGATTATCCGAAGGAATGCCAATCTACTCTTTCCACAACTGCACAAAATTCCACTTCAGATGATCCTAAACAATATCTTAAAGCGGCCATTGTTGCCACTGTTGAAGAGATTACAAAAGGCTTGAATTTAACAGATACCCTTCGAGTGGAGGCTGCTGATAATACCACCATCCAAATGTCTGTTGATGATTGCAAGGACTTATTGCAGTCTGCTATTGACTCGCTTCATGCATCTCAGTCCACTGTTGGGGACCCTGATATGCACACTAATGCAGACCGTGTCGGTGATGTTAAGACATGGCTCAGCGCTGTCATTTCTTTTCAGCAATCTTGTCTCGATGGGCTGGAGGAAACTGATCCCGATCTCAGACAGAAGATGCAAGGCGAGCTTGATTCTGCTGGCAAGCTTACCAGCAATGCTCTTGCTATCCTCGATGAAGTTGTGGGGATATTGGAGAAGCTTGGCTTGCAATTGAAGGTTCAACCAAGTGGGCGTAGGCTTCTTGGGACGACAGAAGTAGGAAGCGATGGGTTCCCGACATGGTTGGTGGGGGAAGACAGGAAGCTGCTGGCAGCTAGAGGAGGCGCCGGGCGAGTGAAGCCCAACGCCGTGGTTGCTAAGGATGGTAGTGGGCAGTTCAAGACCATTGCCGCTGCATTGGCTGCATACCCAAAGGTTCCAACGGGAAGATATGTGATCTATGTGAAAGCAGGGGTGTATGATGAGTACATCATTGTGACTAAAAACATGGAGAACATTTTCATGTATGGAGATGGCCCTAGATCAACTATTGTCACTGGCCGTAAGAGCAACCGCGATGGCGTTACCACCCAAGACAGTGCCTCCTTCTCGGCCATAGGAAATGGATTCCTCTGCAAATCCATGGGATTCAGCAACACAGCCGGCCCCGAAGGCCACCAGGCCGTAGCCCTAAGAGTCCAATCCGACAAGTCCGCGTTCTTCAACTGCAGAATGGACGGGCACCAAGACACCCTGTACGTTCAAGCCAACCGTCAATTCTACAGGAACTGCGTGATCTCTGGCACCGTGGACTTCATCTTCGGCGATTCGTAA

Coding sequence (CDS)

ATGATCGGGAAGGTGGTCGTTTCGGGTGTATCCTTTATTCTCGTCGTAGGTGTAGCTCTTGCCGTCGTTGCAGTCGTTAACAAGAGAAATGACACGGCAGCTGAGATGCAGACTCTGTCCCCAAAGATGAAGGCAGTCGCAGGCATTTGTTCGGGTACTGATTATCCGAAGGAATGCCAATCTACTCTTTCCACAACTGCACAAAATTCCACTTCAGATGATCCTAAACAATATCTTAAAGCGGCCATTGTTGCCACTGTTGAAGAGATTACAAAAGGCTTGAATTTAACAGATACCCTTCGAGTGGAGGCTGCTGATAATACCACCATCCAAATGTCTGTTGATGATTGCAAGGACTTATTGCAGTCTGCTATTGACTCGCTTCATGCATCTCAGTCCACTGTTGGGGACCCTGATATGCACACTAATGCAGACCGTGTCGGTGATGTTAAGACATGGCTCAGCGCTGTCATTTCTTTTCAGCAATCTTGTCTCGATGGGCTGGAGGAAACTGATCCCGATCTCAGACAGAAGATGCAAGGCGAGCTTGATTCTGCTGGCAAGCTTACCAGCAATGCTCTTGCTATCCTCGATGAAGTTGTGGGGATATTGGAGAAGCTTGGCTTGCAATTGAAGGTTCAACCAAGTGGGCGTAGGCTTCTTGGGACGACAGAAGTAGGAAGCGATGGGTTCCCGACATGGTTGGTGGGGGAAGACAGGAAGCTGCTGGCAGCTAGAGGAGGCGCCGGGCGAGTGAAGCCCAACGCCGTGGTTGCTAAGGATGGTAGTGGGCAGTTCAAGACCATTGCCGCTGCATTGGCTGCATACCCAAAGGTTCCAACGGGAAGATATGTGATCTATGTGAAAGCAGGGGTGTATGATGAGTACATCATTGTGACTAAAAACATGGAGAACATTTTCATGTATGGAGATGGCCCTAGATCAACTATTGTCACTGGCCGTAAGAGCAACCGCGATGGCGTTACCACCCAAGACAGTGCCTCCTTCTCGGCCATAGGAAATGGATTCCTCTGCAAATCCATGGGATTCAGCAACACAGCCGGCCCCGAAGGCCACCAGGCCGTAGCCCTAAGAGTCCAATCCGACAAGTCCGCGTTCTTCAACTGCAGAATGGACGGGCACCAAGACACCCTGTACGTTCAAGCCAACCGTCAATTCTACAGGAACTGCGTGATCTCTGGCACCGTGGACTTCATCTTCGGCGATTCGTAA

Protein sequence

MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQSTLSTTAQNSTSDDPKQYLKAAIVATVEEITKGLNLTDTLRVEAADNTTIQMSVDDCKDLLQSAIDSLHASQSTVGDPDMHTNADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQGELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGEDRKLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVPTGRYVIYVKAGVYDEYIIVTKNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGNGFLCKSMGFSNTAGPEGHQAVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDS

Homology

[Close]

BLAST of Carg17713-RA vs. NCBI nr
Match: KAG7032617.1 (putative pectinesterase/pectinesterase inhibitor 21, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 794.3 bits (2050), Expect = 5.1e-226
Identity = 410/410 (100.00%), Postives = 410/410 (100.00%), Query Frame = 0


Query: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ 60
           MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ
Sbjct: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ 60

Query: 61  STLSTTAQNSTSDDPKQYLKAAIVATVEEITKGLNLTDTLRVEAADNTTIQMSVDDCKDL 120
           STLSTTAQNSTSDDPKQYLKAAIVATVEEITKGLNLTDTLRVEAADNTTIQMSVDDCKDL
Sbjct: 61  STLSTTAQNSTSDDPKQYLKAAIVATVEEITKGLNLTDTLRVEAADNTTIQMSVDDCKDL 120

Query: 121 LQSAIDSLHASQSTVGDPDMHTNADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ 180
           LQSAIDSLHASQSTVGDPDMHTNADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ
Sbjct: 121 LQSAIDSLHASQSTVGDPDMHTNADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ 180

Query: 181 GELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGEDR 240
           GELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGEDR
Sbjct: 181 GELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGEDR 240

Query: 241 KLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVPTGRYVIYVKAGVYDEYIIVT 300
           KLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVPTGRYVIYVKAGVYDEYIIVT
Sbjct: 241 KLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVPTGRYVIYVKAGVYDEYIIVT 300

Query: 301 KNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGNGFLCKSMGFSNTAGPEGHQ 360
           KNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGNGFLCKSMGFSNTAGPEGHQ
Sbjct: 301 KNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGNGFLCKSMGFSNTAGPEGHQ 360

Query: 361 AVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDS 411
           AVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDS
Sbjct: 361 AVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDS 410

[Close]

BLAST of Carg17713-RA vs. NCBI nr
Match: KAG6601922.1 (Proline--tRNA ligase, cytoplasmic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 791.2 bits (2042), Expect = 4.3e-225
Identity = 408/410 (99.51%), Postives = 410/410 (100.00%), Query Frame = 0


Query: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ 60
           MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTL+PKMKAVAGICSGTDYPKECQ
Sbjct: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLAPKMKAVAGICSGTDYPKECQ 60

Query: 61  STLSTTAQNSTSDDPKQYLKAAIVATVEEITKGLNLTDTLRVEAADNTTIQMSVDDCKDL 120
           STLSTTAQNSTSDDPKQYLKAAIVATVEEITKGLNLTDTLRVEAADNTTIQMSVDDCKDL
Sbjct: 61  STLSTTAQNSTSDDPKQYLKAAIVATVEEITKGLNLTDTLRVEAADNTTIQMSVDDCKDL 120

Query: 121 LQSAIDSLHASQSTVGDPDMHTNADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ 180
           LQSAIDSLHASQSTVGDPDMHT+ADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ
Sbjct: 121 LQSAIDSLHASQSTVGDPDMHTDADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ 180

Query: 181 GELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGEDR 240
           GELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGEDR
Sbjct: 181 GELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGEDR 240

Query: 241 KLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVPTGRYVIYVKAGVYDEYIIVT 300
           KLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVPTGRYVIYVKAGVYDEYIIVT
Sbjct: 241 KLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVPTGRYVIYVKAGVYDEYIIVT 300

Query: 301 KNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGNGFLCKSMGFSNTAGPEGHQ 360
           KNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGNGFLCKSMGFSNTAGPEGHQ
Sbjct: 301 KNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGNGFLCKSMGFSNTAGPEGHQ 360

Query: 361 AVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDS 411
           AVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDS
Sbjct: 361 AVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDS 410

[Close]

BLAST of Carg17713-RA vs. NCBI nr
Match: XP_022956875.1 (pectinesterase-like [Cucurbita moschata])

HSP 1 Score: 785.8 bits (2028), Expect = 1.8e-223
Identity = 406/410 (99.02%), Postives = 408/410 (99.51%), Query Frame = 0


Query: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ 60
           MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ
Sbjct: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ 60

Query: 61  STLSTTAQNSTSDDPKQYLKAAIVATVEEITKGLNLTDTLRVEAADNTTIQMSVDDCKDL 120
           STLSTTAQNSTSDDPKQYLKAAIVAT+EEITKGLNLTDTLRVEAADNTTIQMSVDDCKDL
Sbjct: 61  STLSTTAQNSTSDDPKQYLKAAIVATIEEITKGLNLTDTLRVEAADNTTIQMSVDDCKDL 120

Query: 121 LQSAIDSLHASQSTVGDPDMHTNADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ 180
           LQSAIDSLHASQSTVGDPDMHT+ADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ
Sbjct: 121 LQSAIDSLHASQSTVGDPDMHTDADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ 180

Query: 181 GELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGEDR 240
           GELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGEDR
Sbjct: 181 GELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGEDR 240

Query: 241 KLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVPTGRYVIYVKAGVYDEYIIVT 300
           KLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKV TGRYVIYVKAGVYDEYIIVT
Sbjct: 241 KLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVLTGRYVIYVKAGVYDEYIIVT 300

Query: 301 KNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGNGFLCKSMGFSNTAGPEGHQ 360
           KNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIG GFLCKSMGFSNTAGPEGHQ
Sbjct: 301 KNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGKGFLCKSMGFSNTAGPEGHQ 360

Query: 361 AVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDS 411
           AVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDS
Sbjct: 361 AVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDS 410

[Close]

BLAST of Carg17713-RA vs. NCBI nr
Match: XP_023527159.1 (pectinesterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 769.6 bits (1986), Expect = 1.3e-218
Identity = 396/410 (96.59%), Postives = 402/410 (98.05%), Query Frame = 0


Query: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ 60
           M+GKVVVSGVSFILVVGVALAVVAVVNKRND A EMQTLSPKMKAVA ICSGTDYPKECQ
Sbjct: 1   MVGKVVVSGVSFILVVGVALAVVAVVNKRNDMATEMQTLSPKMKAVASICSGTDYPKECQ 60

Query: 61  STLSTTAQNSTSDDPKQYLKAAIVATVEEITKGLNLTDTLRVEAADNTTIQMSVDDCKDL 120
           STLS TAQNSTSDDPKQY+KAAIVAT+EEITKGLNLTDTLRVEAADN+TIQMSVDDCKDL
Sbjct: 61  STLSATAQNSTSDDPKQYIKAAIVATIEEITKGLNLTDTLRVEAADNSTIQMSVDDCKDL 120

Query: 121 LQSAIDSLHASQSTVGDPDMHTNADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ 180
           LQSAIDSLHASQSTVGDPDMHT+ADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ
Sbjct: 121 LQSAIDSLHASQSTVGDPDMHTDADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ 180

Query: 181 GELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGEDR 240
           G LDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWL+GEDR
Sbjct: 181 GGLDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLMGEDR 240

Query: 241 KLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVPTGRYVIYVKAGVYDEYIIVT 300
           KLLAARGG GRVKPNAVVAKDGSGQFKTIAAALAAYPKV TGRYVIYVKAGVYDEYIIVT
Sbjct: 241 KLLAARGGTGRVKPNAVVAKDGSGQFKTIAAALAAYPKVLTGRYVIYVKAGVYDEYIIVT 300

Query: 301 KNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGNGFLCKSMGFSNTAGPEGHQ 360
           KNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIG GFLCKSMGFSNTAGPEGHQ
Sbjct: 301 KNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGKGFLCKSMGFSNTAGPEGHQ 360

Query: 361 AVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDS 411
           AVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDS
Sbjct: 361 AVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDS 410

[Close]

BLAST of Carg17713-RA vs. NCBI nr
Match: XP_022990080.1 (pectinesterase-like [Cucurbita maxima])

HSP 1 Score: 763.1 bits (1969), Expect = 1.3e-216
Identity = 392/410 (95.61%), Postives = 402/410 (98.05%), Query Frame = 0


Query: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ 60
           M+GKVVVSGVSFILVVGVALAVVAVVNKRND A EMQTLSPKMKAVA ICSGT+YPKECQ
Sbjct: 1   MVGKVVVSGVSFILVVGVALAVVAVVNKRNDMATEMQTLSPKMKAVASICSGTNYPKECQ 60

Query: 61  STLSTTAQNSTSDDPKQYLKAAIVATVEEITKGLNLTDTLRVEAADNTTIQMSVDDCKDL 120
           STLSTTAQNSTSDDPKQYLKAAIVAT+EEITKGLNLTDTLRVEA DN+TIQMSVDDCKDL
Sbjct: 61  STLSTTAQNSTSDDPKQYLKAAIVATIEEITKGLNLTDTLRVEAVDNSTIQMSVDDCKDL 120

Query: 121 LQSAIDSLHASQSTVGDPDMHTNADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ 180
           LQSAIDSLHASQSTVGDPDMHT+ADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ
Sbjct: 121 LQSAIDSLHASQSTVGDPDMHTDADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ 180

Query: 181 GELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGEDR 240
           GELD+AGKLTSNALAILDEVVG+LEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWL+GEDR
Sbjct: 181 GELDTAGKLTSNALAILDEVVGMLEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLMGEDR 240

Query: 241 KLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVPTGRYVIYVKAGVYDEYIIVT 300
           KLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKV  GRYVIYVKAGVY+EYIIVT
Sbjct: 241 KLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVLRGRYVIYVKAGVYNEYIIVT 300

Query: 301 KNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGNGFLCKSMGFSNTAGPEGHQ 360
           KNMENIFMYGDGPR TIVTGRKSNRDGVTTQDSASFSAIG GFLCKSMGFSNTAGPEGHQ
Sbjct: 301 KNMENIFMYGDGPRLTIVTGRKSNRDGVTTQDSASFSAIGKGFLCKSMGFSNTAGPEGHQ 360

Query: 361 AVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDS 411
           AVALRVQSD+SAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDS
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDS 410

[Close]

BLAST of Carg17713-RA vs. ExPASy Swiss-Prot
Match: O80722 (Pectinesterase 4 OS=Arabidopsis thaliana OX=3702 GN=PME4 PE=2 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 1.8e-80
Identity = 187/447 (41.83%), Postives = 251/447 (56.15%), Query Frame = 0


Query: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ 60
           MIGKVVVS  S +L+VGVA+ VVA +NK  D       LSP+MKAV GIC  T     C 
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFINKNGDA-----NLSPQMKAVQGICQSTSDKASCV 60

Query: 61  STLSTTAQNSTSDDPKQYLKAAIVATVEEITKGLNLTDTLRVEAADNTT--IQMSVDDCK 120
            TL        S+DP + +KA ++AT +E+TK  N T    V    + +   +  +D CK
Sbjct: 61  KTLEPV----KSEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCK 120

Query: 121 DLLQSAIDSLHASQSTVGDPDMHTNADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQK 180
            +   A++ L      +G+ D+     ++  +K WL  V ++Q  CLD +EE   DLR+ 
Sbjct: 121 RVFMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEE--DDLRKA 180

Query: 181 MQGELDSAGKLTSNALAILDEVVGILEKLGLQLK-----------------------VQP 240
           +   + ++  LT+NA+ I   VV  + K+  ++                          P
Sbjct: 181 IGEGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADP 240

Query: 241 SG--RRLLGTTEVGSDGFPTWLVGEDRKLLA--------ARGGAGRVKPNAVVAKDGSGQ 300
            G  RRLL   ++   G PTW+ G DRKL+A         RGG  RV+ N VVAKDGSGQ
Sbjct: 241 DGPARRLL--EDIDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSGQ 300

Query: 301 FKTIAAALAAYPKVPTGRYVIYVKAGVYDEYIIVTKNMENIFMYGDGPRSTIVTGRKS-- 360
           FKT+  A+ A P+   GR +IY+KAG+Y E +I+ K   NIFM+GDG R T+++  +S  
Sbjct: 301 FKTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSVA 360

Query: 361 NRDGVTTQDSASFSAIGNGFLCKSMGFSNTAGPEGHQAVALRVQSDKSAFFNCRMDGHQD 411
              G TT  SA+      GF+ K MGF NTAGP GHQA A+RV  D++  FNCR DG+QD
Sbjct: 361 LSRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQD 420

[Close]

BLAST of Carg17713-RA vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 298.1 bits (762), Expect = 1.5e-79
Identity = 166/412 (40.29%), Postives = 259/412 (62.86%), Query Frame = 0


Query: 4   KVVVSGVSFILVVGVALAVVAVV--NKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQS 63
           + +V  +S +L++ + +AV   V  NK +  +     ++  +KAV  +C+ TDY K C+ 
Sbjct: 14  RYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCED 73

Query: 64  TLSTTAQNSTSDDPKQYLKAAIVATVEEITKGLNLTDTLRVEAADNTTIQMSVDDCKDLL 123
           TL    +N+T  DP + +K A   T+++IT     + T+ +E   ++  +M++D CK+L+
Sbjct: 74  TLIKNGKNTT--DPMELVKTAFNVTMKQITDAAKKSQTI-MELQKDSRTRMALDQCKELM 133

Query: 124 QSAIDSLHASQSTVGDPDMHTNADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQG 183
             A+D L  S   +G  + H   + + +++ WLSA IS +++CL+G + T  +  + M+ 
Sbjct: 134 DYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKK 193

Query: 184 ELDSAGKLTSNALAILDEV---VGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGE 243
            L +A +LT N LAI+ E+   VG ++  GL      + RRLL      ++GFP+W+   
Sbjct: 194 ALKTAIELTHNGLAIISEMSNFVGQMQIPGL------NSRRLL------AEGFPSWVDQR 253

Query: 244 DRKLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVPTGRYVIYVKAGVYDEYII 303
            RKLL A      VKP+ VVA+DGSGQ+KTI  AL   PK     +V+++KAG+Y EY+ 
Sbjct: 254 GRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQ 313

Query: 304 VTKNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGNGFLCKSMGFSNTAGPEG 363
           V K M ++   GDGP  TI++G K+ +DG+TT  +A+ + +GN F+ K++GF NTAG   
Sbjct: 314 VNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIK 373

Query: 364 HQAVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDS 411
           HQAVA+RVQSD+S FFNCR DG+QDTLY  ++RQF+R+C ISGT+DF+FGD+
Sbjct: 374 HQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDA 410

[Close]

BLAST of Carg17713-RA vs. ExPASy Swiss-Prot
Match: Q5MFV8 (Pectinesterase 5 OS=Arabidopsis thaliana OX=3702 GN=PME5 PE=1 SV=2)

HSP 1 Score: 292.0 bits (746), Expect = 1.1e-77
Identity = 185/454 (40.75%), Postives = 253/454 (55.73%), Query Frame = 0


Query: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ 60
           MIGKVVVS  S +L+VGVA+ VVA +NK  D       LSP+MKAV GIC  T     C 
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAYINKNGDA-----NLSPQMKAVRGICEATSDKASCV 60

Query: 61  STLSTTAQNSTSDDPKQYLKAAIVATVEEITKGLNLTDTL--RVEAADNTTIQMSVDDCK 120
            TL        SDDP + +KA ++AT + IT+  N T      + +  +   +  +D CK
Sbjct: 61  KTLEPV----KSDDPNKLIKAFMLATRDAITQSSNFTGKTEGNLGSGISPNNKAVLDYCK 120

Query: 121 DLLQSAIDSLHASQSTVGDPDMHTNADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQK 180
            +   A++ L      +G+ D++    ++  +K WL+ V ++Q  CLD +EE   DLR+ 
Sbjct: 121 KVFMYALEDLSTIVEEMGE-DLNQIGSKIDQLKQWLTGVYNYQTDCLDDIEE--DDLRKT 180

Query: 181 MQGELDSAGKLTSNALAILDEVVGILEKLGLQLK-------------------------- 240
           +   + S+  LTSNA+ I   VV  + KL L+++                          
Sbjct: 181 IGEGIASSKILTSNAIDIFHTVVSAMAKLNLKVEDFKNMTGGIFAPSDKGAAPVNKGTPP 240

Query: 241 -------VQPSG--RRLLGTTEVGSDGFPTWLVGEDRKLL--AARG---GAGRVKPNAVV 300
                    P G  RRLL   ++   G PTW+ G DRKL+  A RG   G  R++   VV
Sbjct: 241 VADDSPVADPDGPARRLL--EDIDETGIPTWVSGADRKLMTKAGRGSNDGGARIRATFVV 300

Query: 301 AKDGSGQFKTIAAALAAYPKVPTGRYVIYVKAGVYDEYIIVTKNMENIFMYGDGPRSTIV 360
           AKDGSGQFKT+  A+ A P+   GR +I++KAG+Y E +I+ K   NIFM+GDG R T++
Sbjct: 301 AKDGSGQFKTVQQAVNACPEKNPGRCIIHIKAGIYREQVIIPKKKNNIFMFGDGARKTVI 360

Query: 361 TGRKSNR--DGVTTQDSASFSAIGNGFLCKSMGFSNTAGPEGHQAVALRVQSDKSAFFNC 411
           +  +S +   G TT  S +      GF+ K +GF NTAGP GHQAVA+RV  D++  FNC
Sbjct: 361 SYNRSVKLSPGTTTSLSGTVQVESEGFMAKWIGFKNTAGPMGHQAVAIRVNGDRAVIFNC 420

[Close]

BLAST of Carg17713-RA vs. ExPASy Swiss-Prot
Match: Q5MFV6 (Probable pectinesterase/pectinesterase inhibitor VGDH2 OS=Arabidopsis thaliana OX=3702 GN=VGDH2 PE=2 SV=2)

HSP 1 Score: 282.3 bits (721), Expect = 8.5e-75
Identity = 177/440 (40.23%), Postives = 246/440 (55.91%), Query Frame = 0


Query: 2   IGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQ-TLSPKMKAVAGICSGTDYPKECQ 61
           +GKVVVS  S +LVVGVA+ V+  VNK      +    ++   KAV  IC  T     C 
Sbjct: 3   VGKVVVSVASLLLVVGVAIGVITFVNKGGGANGDSNGPINSHQKAVQTICQSTTDQGSCA 62

Query: 62  STLSTTAQNSTSDDPKQYLKAAIVATVEEITKGLNLTDTLR--VEAADNTTIQMSVDDCK 121
            TL        SDDP + +KA ++AT + ITK  N T +    +    N T +  +D CK
Sbjct: 63  KTLDPV----KSDDPSKLVKAFLMATKDAITKSSNFTASTEGGMGTNMNATSKAVLDYCK 122

Query: 122 DLLQSAIDSLHASQSTVGDPDMHTNADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQK 181
            +L  A++ L      +G+ D+  +  ++  +K WL+ V ++Q  CLD +EE   +L++ 
Sbjct: 123 RVLMYALEDLETIVEEMGE-DLQQSGTKLDQLKQWLTGVFNYQTDCLDDIEEV--ELKKI 182

Query: 182 MQGELDSAGKLTSNALAILDEVVGILEKLGLQLK----------VQPSGRRLLGTTEVGS 241
           M   + ++  LTSNA+ I   VV  + ++G+++              + RRLL   +  S
Sbjct: 183 MGEGISNSKVLTSNAIDIFHSVVTAMAQMGVKVDDMKNITMGAGAGGAARRLLEDND--S 242

Query: 242 DGFPTWLVGEDRKLLA----------------ARGGAGRVKPNAVVAKDGSGQFKTIAAA 301
            G P W  G+DRKL+A                  GG G++K   VVAKDGSGQFKTI+ A
Sbjct: 243 KGLPKWFSGKDRKLMAKAGRGAPAGGDDGIGEGGGGGGKIKATHVVAKDGSGQFKTISEA 302

Query: 302 LAAYPKVPTGRYVIYVKAGVYDEYIIVTKNMENIFMYGDGPRSTIVTGRKSNR--DGVTT 361
           + A P    GR +I++KAG+Y+E + + K   NIFM+GDG   TI+T  +S +   G TT
Sbjct: 303 VMACPDKNPGRCIIHIKAGIYNEQVRIPKKKNNIFMFGDGATQTIITFDRSVKLSPGTTT 362

Query: 362 QDSASFSAIGNGFLCKSMGFSNTAGPEGHQAVALRVQSDKSAFFNCRMDGHQDTLYVQAN 411
             S +      GF+ K +GF NTAGP GHQAVALRV  D++  FNCR DG+QDTLYV   
Sbjct: 363 SLSGTVQVESEGFMAKWIGFKNTAGPLGHQAVALRVNGDRAVIFNCRFDGYQDTLYVNNG 422

[Close]

BLAST of Carg17713-RA vs. ExPASy Swiss-Prot
Match: Q7Y201 (Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana OX=3702 GN=PME13 PE=2 SV=2)

HSP 1 Score: 280.4 bits (716), Expect = 3.2e-74
Identity = 162/368 (44.02%), Postives = 235/368 (63.86%), Query Frame = 0


Query: 44  KAVAGICSGTDYPKECQSTL-STTAQNSTSDDPKQYLKAAIVATVEEITKGLNLTDTLRV 103
           K +  +CS T Y + C+ TL + T +    D+P  +LK+AI A  E++   L    +L+ 
Sbjct: 107 KIIQTLCSSTLYMQICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDLVLEKVLSLKT 166

Query: 104 EAADNTTIQMSVDDCKDLLQSAIDSLHASQSTVGDPDMHTNADRVGDVKTWLSAVISFQQ 163
           E  D+   + +++ CK L++ A +   AS + +   ++++    V D+++WLSAV+S+Q+
Sbjct: 167 ENQDD---KDAIEQCKLLVEDAKEETVASLNKINVTEVNSFEKVVPDLESWLSAVMSYQE 226

Query: 164 SCLDGLEETDPDLRQKMQGELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLG 223
           +CLDG EE   +L+ +++  ++S+  LTSN+LA++       E L   +KV    R LL 
Sbjct: 227 TCLDGFEE--GNLKSEVKTSVNSSQVLTSNSLALIKT---FTENLSPVMKVVE--RHLL- 286

Query: 224 TTEVGSDGFPTWLVGEDRKLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVPTG 283
                 D  P+W+  +DR++L A      +KPNA VAKDGSG F TI  AL A P+   G
Sbjct: 287 ------DDIPSWVSNDDRRMLRA-VDVKALKPNATVAKDGSGDFTTINDALRAMPEKYEG 346

Query: 284 RYVIYVKAGVYDEYIIVTKNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGNG 343
           RY+IYVK G+YDEY+ V K   N+ M GDG + TIVTG KS+   + T  +A+F A G G
Sbjct: 347 RYIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEG 406

Query: 344 FLCKSMGFSNTAGPEGHQAVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGT 403
           F+ +SMGF NTAGPEGHQAVA+RVQSD+S F NCR +G+QDTLY   +RQ+YR+CVI GT
Sbjct: 407 FMAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGT 456

Query: 404 VDFIFGDS 411
           +DFIFGD+
Sbjct: 467 IDFIFGDA 456

[Close]

BLAST of Carg17713-RA vs. ExPASy TrEMBL
Match: A0A6J1GYZ3 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111458430 PE=3 SV=1)

HSP 1 Score: 785.8 bits (2028), Expect = 8.8e-224
Identity = 406/410 (99.02%), Postives = 408/410 (99.51%), Query Frame = 0


Query: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ 60
           MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ
Sbjct: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ 60

Query: 61  STLSTTAQNSTSDDPKQYLKAAIVATVEEITKGLNLTDTLRVEAADNTTIQMSVDDCKDL 120
           STLSTTAQNSTSDDPKQYLKAAIVAT+EEITKGLNLTDTLRVEAADNTTIQMSVDDCKDL
Sbjct: 61  STLSTTAQNSTSDDPKQYLKAAIVATIEEITKGLNLTDTLRVEAADNTTIQMSVDDCKDL 120

Query: 121 LQSAIDSLHASQSTVGDPDMHTNADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ 180
           LQSAIDSLHASQSTVGDPDMHT+ADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ
Sbjct: 121 LQSAIDSLHASQSTVGDPDMHTDADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ 180

Query: 181 GELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGEDR 240
           GELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGEDR
Sbjct: 181 GELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGEDR 240

Query: 241 KLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVPTGRYVIYVKAGVYDEYIIVT 300
           KLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKV TGRYVIYVKAGVYDEYIIVT
Sbjct: 241 KLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVLTGRYVIYVKAGVYDEYIIVT 300

Query: 301 KNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGNGFLCKSMGFSNTAGPEGHQ 360
           KNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIG GFLCKSMGFSNTAGPEGHQ
Sbjct: 301 KNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGKGFLCKSMGFSNTAGPEGHQ 360

Query: 361 AVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDS 411
           AVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDS
Sbjct: 361 AVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDS 410

[Close]

BLAST of Carg17713-RA vs. ExPASy TrEMBL
Match: A0A6J1JP54 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487084 PE=3 SV=1)

HSP 1 Score: 763.1 bits (1969), Expect = 6.1e-217
Identity = 392/410 (95.61%), Postives = 402/410 (98.05%), Query Frame = 0


Query: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ 60
           M+GKVVVSGVSFILVVGVALAVVAVVNKRND A EMQTLSPKMKAVA ICSGT+YPKECQ
Sbjct: 1   MVGKVVVSGVSFILVVGVALAVVAVVNKRNDMATEMQTLSPKMKAVASICSGTNYPKECQ 60

Query: 61  STLSTTAQNSTSDDPKQYLKAAIVATVEEITKGLNLTDTLRVEAADNTTIQMSVDDCKDL 120
           STLSTTAQNSTSDDPKQYLKAAIVAT+EEITKGLNLTDTLRVEA DN+TIQMSVDDCKDL
Sbjct: 61  STLSTTAQNSTSDDPKQYLKAAIVATIEEITKGLNLTDTLRVEAVDNSTIQMSVDDCKDL 120

Query: 121 LQSAIDSLHASQSTVGDPDMHTNADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ 180
           LQSAIDSLHASQSTVGDPDMHT+ADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ
Sbjct: 121 LQSAIDSLHASQSTVGDPDMHTDADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ 180

Query: 181 GELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGEDR 240
           GELD+AGKLTSNALAILDEVVG+LEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWL+GEDR
Sbjct: 181 GELDTAGKLTSNALAILDEVVGMLEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLMGEDR 240

Query: 241 KLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVPTGRYVIYVKAGVYDEYIIVT 300
           KLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKV  GRYVIYVKAGVY+EYIIVT
Sbjct: 241 KLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVLRGRYVIYVKAGVYNEYIIVT 300

Query: 301 KNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGNGFLCKSMGFSNTAGPEGHQ 360
           KNMENIFMYGDGPR TIVTGRKSNRDGVTTQDSASFSAIG GFLCKSMGFSNTAGPEGHQ
Sbjct: 301 KNMENIFMYGDGPRLTIVTGRKSNRDGVTTQDSASFSAIGKGFLCKSMGFSNTAGPEGHQ 360

Query: 361 AVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDS 411
           AVALRVQSD+SAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDS
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDS 410

[Close]

BLAST of Carg17713-RA vs. ExPASy TrEMBL
Match: A0A0A0KLN2 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G119700 PE=3 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 7.5e-175
Identity = 317/410 (77.32%), Postives = 358/410 (87.32%), Query Frame = 0


Query: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ 60
           M+GKVV+SG+S ILVVGVALAVVA+VNK +D++ E   LSPKMKAVA ICS  DY  ECQ
Sbjct: 1   MVGKVVMSGISLILVVGVALAVVAIVNK-SDSSDETANLSPKMKAVASICSNADYQPECQ 60

Query: 61  STLSTTAQNSTSDDPKQYLKAAIVATVEEITKGLNLTDTLRVEAADNTTIQMSVDDCKDL 120
           +TL +   NS+ +DPK+++KAAI +T+EE+ KG NLTD L VEAA+N TI+MSVDDCKDL
Sbjct: 61  TTLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDL 120

Query: 121 LQSAIDSLHASQSTVGDPDMHTNADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ 180
           LQSAID LHAS STVGDPD+HTN DR+ D+K WL+AVIS+QQSCLDGLEE DP L+QKMQ
Sbjct: 121 LQSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 GELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGEDR 240
             LD AGKLTSNALAI+  V  IL++LGLQLKVQPSGRRLLGTTEV SD FPTWL G DR
Sbjct: 181 DGLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDR 240

Query: 241 KLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVPTGRYVIYVKAGVYDEYIIVT 300
           KLLAA+ G  RVKPN VVAKDGSGQ+KTI AALAAYPK   GRYVIYVKAGVY+E IIVT
Sbjct: 241 KLLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVT 300

Query: 301 KNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGNGFLCKSMGFSNTAGPEGHQ 360
           K+M+NIFMYGDGPR TIVTGRKSNRDG+TTQ++ASF+AIG GFLCKSMGF+NTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDS 411
           AVALRVQSD+SAFFNCRMDG+QDTLYVQ +RQFYRNCVISGTVDFIFGDS
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDS 409

[Close]

BLAST of Carg17713-RA vs. ExPASy TrEMBL
Match: A0A6J1JYH0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111489438 PE=3 SV=1)

HSP 1 Score: 613.2 bits (1580), Expect = 7.8e-172
Identity = 305/411 (74.21%), Postives = 356/411 (86.62%), Query Frame = 0


Query: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ 60
           M+ KVVV+G+S +LVVGVA+AV+AVVNK   + + ++ LSPKMK V+ +CS T+Y  ECQ
Sbjct: 1   MVEKVVVTGMSLMLVVGVAMAVIAVVNKNKSSDSMVENLSPKMKVVSSLCSSTNYQNECQ 60

Query: 61  STLSTTAQNSTSDDPKQYLKAAIVATVEEITKGLNLTDTLRVEAADNTTIQMSVDDCKDL 120
           +TL +TA N+++ DPK Y+KAAI+ATVEEI KG NL++ L VEAA+N TI+MS++DCKDL
Sbjct: 61  NTLGSTAHNASASDPKAYVKAAILATVEEIKKGFNLSNNLMVEAANNATIKMSLEDCKDL 120

Query: 121 LQSAIDSLHASQSTVGDPDMHTNADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ 180
           LQ AID LHAS STVGDP++HT  DR+ D+K WL+AVIS+QQSCLDGLEE DP LR+KMQ
Sbjct: 121 LQYAIDELHASYSTVGDPELHTKEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLRKKMQ 180

Query: 181 GELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGEDR 240
             LD AG+LTSNALA++D V  ILE+ GLQ K++PSGRRLLG TE+GSDGFPTWL   DR
Sbjct: 181 DTLDFAGRLTSNALALVDAVSSILERYGLQFKIKPSGRRLLGATEIGSDGFPTWLSRTDR 240

Query: 241 KLLAAR-GGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVPTGRYVIYVKAGVYDEYIIV 300
           KLLAAR GG GR KPNAVVAKDGSGQFK+IAAALAAYPK   GRYVIYVKAG+Y+EYIIV
Sbjct: 241 KLLAARGGGGGRAKPNAVVAKDGSGQFKSIAAALAAYPKGSKGRYVIYVKAGIYNEYIIV 300

Query: 301 TKNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGNGFLCKSMGFSNTAGPEGH 360
           TK M N+FMYGDGPR T+VTGRK NRDG+TTQD+ASFSA+GNGFLCKSMGF NTAGP+GH
Sbjct: 301 TKEMRNVFMYGDGPRKTLVTGRKCNRDGITTQDTASFSALGNGFLCKSMGFQNTAGPQGH 360

Query: 361 QAVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDS 411
           QAVALRVQSDKSAFFNCRMDG+QDTLYVQ +RQFYRNCVISGTVDFIFGDS
Sbjct: 361 QAVALRVQSDKSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDS 411

[Close]

BLAST of Carg17713-RA vs. ExPASy TrEMBL
Match: A0A5A7U7W4 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00090 PE=3 SV=1)

HSP 1 Score: 610.1 bits (1572), Expect = 6.6e-171
Identity = 309/410 (75.37%), Postives = 357/410 (87.07%), Query Frame = 0


Query: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ 60
           M+ KVV++ V+ +LVVGV LAV A+VNK N ++ E   +SPKMKAV+ ICS TDY +ECQ
Sbjct: 1   MVAKVVMA-VTSLLVVGVVLAVFAMVNK-NHSSDETADMSPKMKAVSSICSNTDYQQECQ 60

Query: 61  STLSTTAQNSTSDDPKQYLKAAIVATVEEITKGLNLTDTLRVEAADNTTIQMSVDDCKDL 120
           +TL     NS+ DDPK++LKAAI AT+EE+ KG NLTD L VEAA+N +I+M+VDDC+DL
Sbjct: 61  TTLGNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDL 120

Query: 121 LQSAIDSLHASQSTVGDPDMHTNADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ 180
           LQSAID LHAS STVGDPD+HTN DRV D+K WL+AVIS+QQSCLDGLEE DP L+QKMQ
Sbjct: 121 LQSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 GELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGEDR 240
           G LD A KLTSNALAI+D V  IL+ LGLQLKVQPSGRRLLGTTEV +DG+PTWL G DR
Sbjct: 181 GGLDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGYPTWLTGADR 240

Query: 241 KLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVPTGRYVIYVKAGVYDEYIIVT 300
           KLLAA+ G  RVKPNAVVAKDGSGQ+KTIAAALAAYPK   GRYVIYVKAG+Y+EYIIVT
Sbjct: 241 KLLAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVT 300

Query: 301 KNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGNGFLCKSMGFSNTAGPEGHQ 360
           K+M+N+FMYGDGPR TIVTGRKSNRDG+ TQ++A+F+AIG GFLCKSMGF+NTAGPEGHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDS 411
           AVALRVQSD+SAFFNCRMDG+QDTLYVQ +RQFYRNCV+SGTVDFIFGDS
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDS 408

[Close]

BLAST of Carg17713-RA vs. TAIR 10
Match: AT2G47030.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 301.2 bits (770), Expect = 1.3e-81
Identity = 187/447 (41.83%), Postives = 251/447 (56.15%), Query Frame = 0


Query: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ 60
           MIGKVVVS  S +L+VGVA+ VVA +NK  D       LSP+MKAV GIC  T     C 
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFINKNGDA-----NLSPQMKAVQGICQSTSDKASCV 60

Query: 61  STLSTTAQNSTSDDPKQYLKAAIVATVEEITKGLNLTDTLRVEAADNTT--IQMSVDDCK 120
            TL        S+DP + +KA ++AT +E+TK  N T    V    + +   +  +D CK
Sbjct: 61  KTLEPV----KSEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCK 120

Query: 121 DLLQSAIDSLHASQSTVGDPDMHTNADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQK 180
            +   A++ L      +G+ D+     ++  +K WL  V ++Q  CLD +EE   DLR+ 
Sbjct: 121 RVFMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEE--DDLRKA 180

Query: 181 MQGELDSAGKLTSNALAILDEVVGILEKLGLQLK-----------------------VQP 240
           +   + ++  LT+NA+ I   VV  + K+  ++                          P
Sbjct: 181 IGEGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADP 240

Query: 241 SG--RRLLGTTEVGSDGFPTWLVGEDRKLLA--------ARGGAGRVKPNAVVAKDGSGQ 300
            G  RRLL   ++   G PTW+ G DRKL+A         RGG  RV+ N VVAKDGSGQ
Sbjct: 241 DGPARRLL--EDIDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSGQ 300

Query: 301 FKTIAAALAAYPKVPTGRYVIYVKAGVYDEYIIVTKNMENIFMYGDGPRSTIVTGRKS-- 360
           FKT+  A+ A P+   GR +IY+KAG+Y E +I+ K   NIFM+GDG R T+++  +S  
Sbjct: 301 FKTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSVA 360

Query: 361 NRDGVTTQDSASFSAIGNGFLCKSMGFSNTAGPEGHQAVALRVQSDKSAFFNCRMDGHQD 411
              G TT  SA+      GF+ K MGF NTAGP GHQA A+RV  D++  FNCR DG+QD
Sbjct: 361 LSRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQD 420

[Close]

BLAST of Carg17713-RA vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 298.1 bits (762), Expect = 1.1e-80
Identity = 166/412 (40.29%), Postives = 259/412 (62.86%), Query Frame = 0


Query: 4   KVVVSGVSFILVVGVALAVVAVV--NKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQS 63
           + +V  +S +L++ + +AV   V  NK +  +     ++  +KAV  +C+ TDY K C+ 
Sbjct: 14  RYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCED 73

Query: 64  TLSTTAQNSTSDDPKQYLKAAIVATVEEITKGLNLTDTLRVEAADNTTIQMSVDDCKDLL 123
           TL    +N+T  DP + +K A   T+++IT     + T+ +E   ++  +M++D CK+L+
Sbjct: 74  TLIKNGKNTT--DPMELVKTAFNVTMKQITDAAKKSQTI-MELQKDSRTRMALDQCKELM 133

Query: 124 QSAIDSLHASQSTVGDPDMHTNADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQG 183
             A+D L  S   +G  + H   + + +++ WLSA IS +++CL+G + T  +  + M+ 
Sbjct: 134 DYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKK 193

Query: 184 ELDSAGKLTSNALAILDEV---VGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGE 243
            L +A +LT N LAI+ E+   VG ++  GL      + RRLL      ++GFP+W+   
Sbjct: 194 ALKTAIELTHNGLAIISEMSNFVGQMQIPGL------NSRRLL------AEGFPSWVDQR 253

Query: 244 DRKLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVPTGRYVIYVKAGVYDEYII 303
            RKLL A      VKP+ VVA+DGSGQ+KTI  AL   PK     +V+++KAG+Y EY+ 
Sbjct: 254 GRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQ 313

Query: 304 VTKNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGNGFLCKSMGFSNTAGPEG 363
           V K M ++   GDGP  TI++G K+ +DG+TT  +A+ + +GN F+ K++GF NTAG   
Sbjct: 314 VNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIK 373

Query: 364 HQAVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDS 411
           HQAVA+RVQSD+S FFNCR DG+QDTLY  ++RQF+R+C ISGT+DF+FGD+
Sbjct: 374 HQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDA 410

[Close]

BLAST of Carg17713-RA vs. TAIR 10
Match: AT2G47040.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 292.0 bits (746), Expect = 7.6e-79
Identity = 185/454 (40.75%), Postives = 253/454 (55.73%), Query Frame = 0


Query: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ 60
           MIGKVVVS  S +L+VGVA+ VVA +NK  D       LSP+MKAV GIC  T     C 
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAYINKNGDA-----NLSPQMKAVRGICEATSDKASCV 60

Query: 61  STLSTTAQNSTSDDPKQYLKAAIVATVEEITKGLNLTDTL--RVEAADNTTIQMSVDDCK 120
            TL        SDDP + +KA ++AT + IT+  N T      + +  +   +  +D CK
Sbjct: 61  KTLEPV----KSDDPNKLIKAFMLATRDAITQSSNFTGKTEGNLGSGISPNNKAVLDYCK 120

Query: 121 DLLQSAIDSLHASQSTVGDPDMHTNADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQK 180
            +   A++ L      +G+ D++    ++  +K WL+ V ++Q  CLD +EE   DLR+ 
Sbjct: 121 KVFMYALEDLSTIVEEMGE-DLNQIGSKIDQLKQWLTGVYNYQTDCLDDIEE--DDLRKT 180

Query: 181 MQGELDSAGKLTSNALAILDEVVGILEKLGLQLK-------------------------- 240
           +   + S+  LTSNA+ I   VV  + KL L+++                          
Sbjct: 181 IGEGIASSKILTSNAIDIFHTVVSAMAKLNLKVEDFKNMTGGIFAPSDKGAAPVNKGTPP 240

Query: 241 -------VQPSG--RRLLGTTEVGSDGFPTWLVGEDRKLL--AARG---GAGRVKPNAVV 300
                    P G  RRLL   ++   G PTW+ G DRKL+  A RG   G  R++   VV
Sbjct: 241 VADDSPVADPDGPARRLL--EDIDETGIPTWVSGADRKLMTKAGRGSNDGGARIRATFVV 300

Query: 301 AKDGSGQFKTIAAALAAYPKVPTGRYVIYVKAGVYDEYIIVTKNMENIFMYGDGPRSTIV 360
           AKDGSGQFKT+  A+ A P+   GR +I++KAG+Y E +I+ K   NIFM+GDG R T++
Sbjct: 301 AKDGSGQFKTVQQAVNACPEKNPGRCIIHIKAGIYREQVIIPKKKNNIFMFGDGARKTVI 360

Query: 361 TGRKSNR--DGVTTQDSASFSAIGNGFLCKSMGFSNTAGPEGHQAVALRVQSDKSAFFNC 411
           +  +S +   G TT  S +      GF+ K +GF NTAGP GHQAVA+RV  D++  FNC
Sbjct: 361 SYNRSVKLSPGTTTSLSGTVQVESEGFMAKWIGFKNTAGPMGHQAVAIRVNGDRAVIFNC 420

[Close]

BLAST of Carg17713-RA vs. TAIR 10
Match: AT3G62170.1 (VANGUARD 1 homolog 2 )

HSP 1 Score: 282.3 bits (721), Expect = 6.0e-76
Identity = 177/440 (40.23%), Postives = 246/440 (55.91%), Query Frame = 0


Query: 2   IGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQ-TLSPKMKAVAGICSGTDYPKECQ 61
           +GKVVVS  S +LVVGVA+ V+  VNK      +    ++   KAV  IC  T     C 
Sbjct: 3   VGKVVVSVASLLLVVGVAIGVITFVNKGGGANGDSNGPINSHQKAVQTICQSTTDQGSCA 62

Query: 62  STLSTTAQNSTSDDPKQYLKAAIVATVEEITKGLNLTDTLR--VEAADNTTIQMSVDDCK 121
            TL        SDDP + +KA ++AT + ITK  N T +    +    N T +  +D CK
Sbjct: 63  KTLDPV----KSDDPSKLVKAFLMATKDAITKSSNFTASTEGGMGTNMNATSKAVLDYCK 122

Query: 122 DLLQSAIDSLHASQSTVGDPDMHTNADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQK 181
            +L  A++ L      +G+ D+  +  ++  +K WL+ V ++Q  CLD +EE   +L++ 
Sbjct: 123 RVLMYALEDLETIVEEMGE-DLQQSGTKLDQLKQWLTGVFNYQTDCLDDIEEV--ELKKI 182

Query: 182 MQGELDSAGKLTSNALAILDEVVGILEKLGLQLK----------VQPSGRRLLGTTEVGS 241
           M   + ++  LTSNA+ I   VV  + ++G+++              + RRLL   +  S
Sbjct: 183 MGEGISNSKVLTSNAIDIFHSVVTAMAQMGVKVDDMKNITMGAGAGGAARRLLEDND--S 242

Query: 242 DGFPTWLVGEDRKLLA----------------ARGGAGRVKPNAVVAKDGSGQFKTIAAA 301
            G P W  G+DRKL+A                  GG G++K   VVAKDGSGQFKTI+ A
Sbjct: 243 KGLPKWFSGKDRKLMAKAGRGAPAGGDDGIGEGGGGGGKIKATHVVAKDGSGQFKTISEA 302

Query: 302 LAAYPKVPTGRYVIYVKAGVYDEYIIVTKNMENIFMYGDGPRSTIVTGRKSNR--DGVTT 361
           + A P    GR +I++KAG+Y+E + + K   NIFM+GDG   TI+T  +S +   G TT
Sbjct: 303 VMACPDKNPGRCIIHIKAGIYNEQVRIPKKKNNIFMFGDGATQTIITFDRSVKLSPGTTT 362

Query: 362 QDSASFSAIGNGFLCKSMGFSNTAGPEGHQAVALRVQSDKSAFFNCRMDGHQDTLYVQAN 411
             S +      GF+ K +GF NTAGP GHQAVALRV  D++  FNCR DG+QDTLYV   
Sbjct: 363 SLSGTVQVESEGFMAKWIGFKNTAGPLGHQAVALRVNGDRAVIFNCRFDGYQDTLYVNNG 422

[Close]

BLAST of Carg17713-RA vs. TAIR 10
Match: AT2G26450.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 280.4 bits (716), Expect = 2.3e-75
Identity = 162/368 (44.02%), Postives = 235/368 (63.86%), Query Frame = 0


Query: 44  KAVAGICSGTDYPKECQSTL-STTAQNSTSDDPKQYLKAAIVATVEEITKGLNLTDTLRV 103
           K +  +CS T Y + C+ TL + T +    D+P  +LK+AI A  E++   L    +L+ 
Sbjct: 107 KIIQTLCSSTLYMQICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDLVLEKVLSLKT 166

Query: 104 EAADNTTIQMSVDDCKDLLQSAIDSLHASQSTVGDPDMHTNADRVGDVKTWLSAVISFQQ 163
           E  D+   + +++ CK L++ A +   AS + +   ++++    V D+++WLSAV+S+Q+
Sbjct: 167 ENQDD---KDAIEQCKLLVEDAKEETVASLNKINVTEVNSFEKVVPDLESWLSAVMSYQE 226

Query: 164 SCLDGLEETDPDLRQKMQGELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLG 223
           +CLDG EE   +L+ +++  ++S+  LTSN+LA++       E L   +KV    R LL 
Sbjct: 227 TCLDGFEE--GNLKSEVKTSVNSSQVLTSNSLALIKT---FTENLSPVMKVVE--RHLL- 286

Query: 224 TTEVGSDGFPTWLVGEDRKLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVPTG 283
                 D  P+W+  +DR++L A      +KPNA VAKDGSG F TI  AL A P+   G
Sbjct: 287 ------DDIPSWVSNDDRRMLRA-VDVKALKPNATVAKDGSGDFTTINDALRAMPEKYEG 346

Query: 284 RYVIYVKAGVYDEYIIVTKNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGNG 343
           RY+IYVK G+YDEY+ V K   N+ M GDG + TIVTG KS+   + T  +A+F A G G
Sbjct: 347 RYIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEG 406

Query: 344 FLCKSMGFSNTAGPEGHQAVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGT 403
           F+ +SMGF NTAGPEGHQAVA+RVQSD+S F NCR +G+QDTLY   +RQ+YR+CVI GT
Sbjct: 407 FMAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGT 456

Query: 404 VDFIFGDS 411
           +DFIFGD+
Sbjct: 467 IDFIFGDA 456

The following BLAST results are available for this feature:

BLAST of Carg17713-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7032617.1	5.1e-226	100.00	putative pectinesterase/pectinesterase inhibitor 21, partial [Cucurbita argyrosp...	[more]
KAG6601922.1	4.3e-225	99.51	Proline--tRNA ligase, cytoplasmic, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022956875.1	1.8e-223	99.02	pectinesterase-like [Cucurbita moschata]	[more]
XP_023527159.1	1.3e-218	96.59	pectinesterase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022990080.1	1.3e-216	95.61	pectinesterase-like [Cucurbita maxima]	[more]

BLAST of Carg17713-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O80722	1.8e-80	41.83	Pectinesterase 4 OS=Arabidopsis thaliana OX=3702 GN=PME4 PE=2 SV=1	[more]
Q8GX86	1.5e-79	40.29	Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3...	[more]
Q5MFV8	1.1e-77	40.75	Pectinesterase 5 OS=Arabidopsis thaliana OX=3702 GN=PME5 PE=1 SV=2	[more]
Q5MFV6	8.5e-75	40.23	Probable pectinesterase/pectinesterase inhibitor VGDH2 OS=Arabidopsis thaliana O...	[more]
Q7Y201	3.2e-74	44.02	Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Carg17713-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GYZ3	8.8e-224	99.02	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111458430 PE=3 SV=1	[more]
A0A6J1JP54	6.1e-217	95.61	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487084 PE=3 SV=1	[more]
A0A0A0KLN2	7.5e-175	77.32	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G119700 PE=3 SV=1	[more]
A0A6J1JYH0	7.8e-172	74.21	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111489438 PE=3 SV=1	[more]
A0A5A7U7W4	6.6e-171	75.37	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00090...	[more]

BLAST of Carg17713-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G47030.1	1.3e-81	41.83	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G05610.1	1.1e-80	40.29	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT2G47040.1	7.6e-79	40.75	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G62170.1	6.0e-76	40.23	VANGUARD 1 homolog 2	[more]
AT2G26450.1	2.3e-75	44.02	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 41..196 e-value: 1.5E-34 score: 130.8
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 36..199 e-value: 2.5E-21 score: 73.8
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 45..196 e-value: 7.4E-27 score: 94.5
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 249..410 e-value: 3.0E-59 score: 203.0
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 43..200 e-value: 2.1E-31 score: 110.9
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 45..198
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 255..410 e-value: 3.5E-67 score: 226.2
None	No IPR available	PANTHER	PTHR31707:SF164	PECTINESTERASE 4-RELATED	coord: 5..410
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 5..410
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 49..199 e-value: 1.07792E-45 score: 152.978
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 399..408
IPR018040	Pectinesterase, Tyr active site	PROSITE	PS00800	PECTINESTERASE_1	coord: 280..299
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 253..409

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg17713	Carg17713	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg17713-RA:1	Carg17713-RA:1	exon
Carg17713-RA:2	Carg17713-RA:2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg17713-RA:cds	Carg17713-RA:cds	CDS
Carg17713-RA:cds	Carg17713-RA:cds_2	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg17713-RA	Carg17713-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity