Carg17521-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg17521-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Cytochrome P450
Location	Carg_Chr12: 11411729 .. 11413467 (-)
Sequence length	1011
RNA-Seq Expression	Carg17521-RA
Synteny	Carg17521-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTATAAACACAGGAAGTCCCAAAACCCAAAATCCTCGCAACATCAAGATGGTGGGTGGCGTTTTGGAGTGGATTAAAGTTTCGATTTTGGGCTGTTTTTTGGTGGTGGTGGCGCTGAGAGCGGCGGTGGTGCTGTGGTGGCGGCCGAGGAGAATCGAAGACCATTTTTTCCGGCAAGGAATTAGAGGACCTCCTTATCGTTTCTTCATCGGAAATGTTAAGGAACTTGTCGGAATGATGATTAAAGCTTCTTCTGATCATTCTTTTCCTAATTTTTCTCATAACATTCTTCCTCGTGTTCTTCCGTTTTATCATCACTGGAAGAAGATTTATGGTACTGTTTTTCTCTCTCTCTCTCTCTCTCTAGAATTTGCCACTTTTTTGGTGGATGTTTGGTTTTTTTTTTGTTGAAATTTTGGCTTCCATTTGTTTGTTTTGGCTCTTTTCAAGCTTCCCTCTCGTTTTCTCTTTAAAAGTTATCTTCTTCTTCATTGAAAAACACTGTCCAAAAGCACTTTTACCACCTAAAATTTGTTCCATACCTGACCGAATTTGATTTTGTGTCTAATAGGTTCGAAATTTTTGGTGTGGTTCGGGCCGACGGTGAGATTAGCGGTGTCTGATCCGGACCTCATTCGAGAAATCTTCACATCAAAATCAGAATTCTGTGAGAAAAATGAACCTCACCCACTGGTTAAACAGCTAGAAGGTGATGGCCTCCTCAGCCTCAAAGGCCAAAAATGGGCTCTTCATCGCAAGATCATCTCTCCTTCTTTCCACATGGACAATCTCAAAGTACCTATCTTATTCTATTTCTCTCTCCCTCTCTATCATTTTTCTCAAATGGGGTCATTAAAAAAATTCAATTTTCTCAAATGGGCAGTAAAAAAAATCGAATTTTCTTAAATGGGTCGTGAAGAAATTCAATTTTCTCATATGGGGTCGTAAAAAATTGAATTTTCTCAAATGGGGTCGTGCAAAAATTGAATTTTTTTCATATGGGGTCGTGAAAAAATTCAGTTTTCTCAAATGGGGTCGTGAAAAAATTCAGTTTTCTCAAATGGGATCGTGAAAAATTTGAATTTTCTCATATGGGGTCGTGAAAATTTGATTTTTCTCAAATGGGTATCGAAAGAAATGTCTTTTTTTTTACACATTTTTATGATTTTTTCTTCTCTTTTTTGGATTAGTTGTTGATTCCAGTGATGGCAAAAAGTGTCGTGGATATGTTGGAGAAATGGTCAGCAATGTCAGCAGCTTCCGGTGAGGTGGAAATTGAAGTTTCCGAATGGTTCCAAACCCTAACCGAAGACGTCATCACTAGGACTGCCTTTGGCTCCAGCTATGAAGATGGAAAAGCCATTTTTCGCTTACAAGCTCAACAAATGGTCCTTGCTTCCCAAGCCTTCGAGAAAGTTTTCATCCCTGGCTACAGGTACCCCCGCTACTCAACGGTTCGATTCATTTGTTAACAACGCTCGATAGTTTCGTTGATTAGTAATTTAACATGCACGATTCGGATGTATGTTAGATTCTTACCGACGAGAACAAACGTGAATTCGTGGAGACTAGATCGAGAAATAAGAAAATCGTTGTTGAAACTGATCGACCGACGTCGAGAGAGCTCGATCGAGAGCTCAAAGGATTTACTAGGGTTAATGATAAGGGCATCAAACACTTCTCCTTCATCAACTATCACTGTTAATGACATTGTTGAAGAGTGCAAAGGGTTCTTCTTT

mRNA sequence

ATTATAAACACAGGAAGTCCCAAAACCCAAAATCCTCGCAACATCAAGATGGTGGGTGGCGTTTTGGAGTGGATTAAAGTTTCGATTTTGGGCTGTTTTTTGGTGGTGGTGGCGCTGAGAGCGGCGGTGGTGCTGTGGTGGCGGCCGAGGAGAATCGAAGACCATTTTTTCCGGCAAGGAATTAGAGGACCTCCTTATCGTTTCTTCATCGGAAATGTTAAGGAACTTGTCGGAATGATGATTAAAGCTTCTTCTGATCATTCTTTTCCTAATTTTTCTCATAACATTCTTCCTCGTGTTCTTCCGTTTTATCATCACTGGAAGAAGATTTATGGTTCGAAATTTTTGGTGTGGTTCGGGCCGACGGTGAGATTAGCGGTGTCTGATCCGGACCTCATTCGAGAAATCTTCACATCAAAATCAGAATTCTGTGAGAAAAATGAACCTCACCCACTGGTTAAACAGCTAGAAGGTGATGGCCTCCTCAGCCTCAAAGGCCAAAAATGGGCTCTTCATCGCAAGATCATCTCTCCTTCTTTCCACATGGACAATCTCAAATTGTTGATTCCAGTGATGGCAAAAAGTGTCGTGGATATGTTGGAGAAATGGTCAGCAATGTCAGCAGCTTCCGGTGAGGTGGAAATTGAAGTTTCCGAATGGTTCCAAACCCTAACCGAAGACGTCATCACTAGGACTGCCTTTGGCTCCAGCTATGAAGATGGAAAAGCCATTTTTCGCTTACAAGCTCAACAAATGGTCCTTGCTTCCCAAGCCTTCGAGAAAGTTTTCATCCCTGGCTACAGATTCTTACCGACGAGAACAAACGTGAATTCGTGGAGACTAGATCGAGAAATAAGAAAATCGTTGTTGAAACTGATCGACCGACGTCGAGAGAGCTCGATCGAGAGCTCAAAGGATTTACTAGGGTTAATGATAAGGGCATCAAACACTTCTCCTTCATCAACTATCACTGTTAATGACATTGTTGAAGAGTGCAAAGGGTTCTTCTTT

Coding sequence (CDS)

ATGGTGGGTGGCGTTTTGGAGTGGATTAAAGTTTCGATTTTGGGCTGTTTTTTGGTGGTGGTGGCGCTGAGAGCGGCGGTGGTGCTGTGGTGGCGGCCGAGGAGAATCGAAGACCATTTTTTCCGGCAAGGAATTAGAGGACCTCCTTATCGTTTCTTCATCGGAAATGTTAAGGAACTTGTCGGAATGATGATTAAAGCTTCTTCTGATCATTCTTTTCCTAATTTTTCTCATAACATTCTTCCTCGTGTTCTTCCGTTTTATCATCACTGGAAGAAGATTTATGGTTCGAAATTTTTGGTGTGGTTCGGGCCGACGGTGAGATTAGCGGTGTCTGATCCGGACCTCATTCGAGAAATCTTCACATCAAAATCAGAATTCTGTGAGAAAAATGAACCTCACCCACTGGTTAAACAGCTAGAAGGTGATGGCCTCCTCAGCCTCAAAGGCCAAAAATGGGCTCTTCATCGCAAGATCATCTCTCCTTCTTTCCACATGGACAATCTCAAATTGTTGATTCCAGTGATGGCAAAAAGTGTCGTGGATATGTTGGAGAAATGGTCAGCAATGTCAGCAGCTTCCGGTGAGGTGGAAATTGAAGTTTCCGAATGGTTCCAAACCCTAACCGAAGACGTCATCACTAGGACTGCCTTTGGCTCCAGCTATGAAGATGGAAAAGCCATTTTTCGCTTACAAGCTCAACAAATGGTCCTTGCTTCCCAAGCCTTCGAGAAAGTTTTCATCCCTGGCTACAGATTCTTACCGACGAGAACAAACGTGAATTCGTGGAGACTAGATCGAGAAATAAGAAAATCGTTGTTGAAACTGATCGACCGACGTCGAGAGAGCTCGATCGAGAGCTCAAAGGATTTACTAGGGTTAATGATAAGGGCATCAAACACTTCTCCTTCATCAACTATCACTGTTAATGACATTGTTGAAGAGTGCAAAGGGTTCTTCTTT

Protein sequence

MVGGVLEWIKVSILGCFLVVVALRAAVVLWWRPRRIEDHFFRQGIRGPPYRFFIGNVKELVGMMIKASSDHSFPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMLEKWSAMSAASGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLASQAFEKVFIPGYRFLPTRTNVNSWRLDREIRKSLLKLIDRRRESSIESSKDLLGLMIRASNTSPSSTITVNDIVEECKGFFF

Homology

[Close]

BLAST of Carg17521-RA vs. NCBI nr
Match: XP_023537030.1 (cytochrome P450 734A1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 646.7 bits (1667), Expect = 1.0e-181
Identity = 321/321 (100.00%), Postives = 321/321 (100.00%), Query Frame = 0


Query: 1   MVGGVLEWIKVSILGCFLVVVALRAAVVLWWRPRRIEDHFFRQGIRGPPYRFFIGNVKEL 60
           MVGGVLEWIKVSILGCFLVVVALRAAVVLWWRPRRIEDHFFRQGIRGPPYRFFIGNVKEL
Sbjct: 1   MVGGVLEWIKVSILGCFLVVVALRAAVVLWWRPRRIEDHFFRQGIRGPPYRFFIGNVKEL 60

Query: 61  VGMMIKASSDHSFPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREI 120
           VGMMIKASSDHSFPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREI
Sbjct: 61  VGMMIKASSDHSFPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREI 120

Query: 121 FTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSV 180
           FTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSV
Sbjct: 121 FTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSV 180

Query: 181 VDMLEKWSAMSAASGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLAS 240
           VDMLEKWSAMSAASGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLAS
Sbjct: 181 VDMLEKWSAMSAASGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLAS 240

Query: 241 QAFEKVFIPGYRFLPTRTNVNSWRLDREIRKSLLKLIDRRRESSIESSKDLLGLMIRASN 300
           QAFEKVFIPGYRFLPTRTNVNSWRLDREIRKSLLKLIDRRRESSIESSKDLLGLMIRASN
Sbjct: 241 QAFEKVFIPGYRFLPTRTNVNSWRLDREIRKSLLKLIDRRRESSIESSKDLLGLMIRASN 300

Query: 301 TSPSSTITVNDIVEECKGFFF 322
           TSPSSTITVNDIVEECKGFFF
Sbjct: 301 TSPSSTITVNDIVEECKGFFF 321

[Close]

BLAST of Carg17521-RA vs. NCBI nr
Match: KAG7021263.1 (Cytochrome P450, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 646.7 bits (1667), Expect = 1.0e-181
Identity = 321/321 (100.00%), Postives = 321/321 (100.00%), Query Frame = 0


Query: 1   MVGGVLEWIKVSILGCFLVVVALRAAVVLWWRPRRIEDHFFRQGIRGPPYRFFIGNVKEL 60
           MVGGVLEWIKVSILGCFLVVVALRAAVVLWWRPRRIEDHFFRQGIRGPPYRFFIGNVKEL
Sbjct: 1   MVGGVLEWIKVSILGCFLVVVALRAAVVLWWRPRRIEDHFFRQGIRGPPYRFFIGNVKEL 60

Query: 61  VGMMIKASSDHSFPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREI 120
           VGMMIKASSDHSFPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREI
Sbjct: 61  VGMMIKASSDHSFPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREI 120

Query: 121 FTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSV 180
           FTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSV
Sbjct: 121 FTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSV 180

Query: 181 VDMLEKWSAMSAASGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLAS 240
           VDMLEKWSAMSAASGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLAS
Sbjct: 181 VDMLEKWSAMSAASGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLAS 240

Query: 241 QAFEKVFIPGYRFLPTRTNVNSWRLDREIRKSLLKLIDRRRESSIESSKDLLGLMIRASN 300
           QAFEKVFIPGYRFLPTRTNVNSWRLDREIRKSLLKLIDRRRESSIESSKDLLGLMIRASN
Sbjct: 241 QAFEKVFIPGYRFLPTRTNVNSWRLDREIRKSLLKLIDRRRESSIESSKDLLGLMIRASN 300

Query: 301 TSPSSTITVNDIVEECKGFFF 322
           TSPSSTITVNDIVEECKGFFF
Sbjct: 301 TSPSSTITVNDIVEECKGFFF 321

[Close]

BLAST of Carg17521-RA vs. NCBI nr
Match: XP_022937875.1 (cytochrome P450 734A1-like [Cucurbita moschata])

HSP 1 Score: 646.7 bits (1667), Expect = 1.0e-181
Identity = 321/321 (100.00%), Postives = 321/321 (100.00%), Query Frame = 0


Query: 1   MVGGVLEWIKVSILGCFLVVVALRAAVVLWWRPRRIEDHFFRQGIRGPPYRFFIGNVKEL 60
           MVGGVLEWIKVSILGCFLVVVALRAAVVLWWRPRRIEDHFFRQGIRGPPYRFFIGNVKEL
Sbjct: 1   MVGGVLEWIKVSILGCFLVVVALRAAVVLWWRPRRIEDHFFRQGIRGPPYRFFIGNVKEL 60

Query: 61  VGMMIKASSDHSFPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREI 120
           VGMMIKASSDHSFPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREI
Sbjct: 61  VGMMIKASSDHSFPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREI 120

Query: 121 FTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSV 180
           FTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSV
Sbjct: 121 FTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSV 180

Query: 181 VDMLEKWSAMSAASGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLAS 240
           VDMLEKWSAMSAASGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLAS
Sbjct: 181 VDMLEKWSAMSAASGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLAS 240

Query: 241 QAFEKVFIPGYRFLPTRTNVNSWRLDREIRKSLLKLIDRRRESSIESSKDLLGLMIRASN 300
           QAFEKVFIPGYRFLPTRTNVNSWRLDREIRKSLLKLIDRRRESSIESSKDLLGLMIRASN
Sbjct: 241 QAFEKVFIPGYRFLPTRTNVNSWRLDREIRKSLLKLIDRRRESSIESSKDLLGLMIRASN 300

Query: 301 TSPSSTITVNDIVEECKGFFF 322
           TSPSSTITVNDIVEECKGFFF
Sbjct: 301 TSPSSTITVNDIVEECKGFFF 321

[Close]

BLAST of Carg17521-RA vs. NCBI nr
Match: KAG6586408.1 (Cytochrome P450 734A1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 646.7 bits (1667), Expect = 1.0e-181
Identity = 321/321 (100.00%), Postives = 321/321 (100.00%), Query Frame = 0


Query: 1   MVGGVLEWIKVSILGCFLVVVALRAAVVLWWRPRRIEDHFFRQGIRGPPYRFFIGNVKEL 60
           MVGGVLEWIKVSILGCFLVVVALRAAVVLWWRPRRIEDHFFRQGIRGPPYRFFIGNVKEL
Sbjct: 1   MVGGVLEWIKVSILGCFLVVVALRAAVVLWWRPRRIEDHFFRQGIRGPPYRFFIGNVKEL 60

Query: 61  VGMMIKASSDHSFPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREI 120
           VGMMIKASSDHSFPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREI
Sbjct: 61  VGMMIKASSDHSFPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREI 120

Query: 121 FTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSV 180
           FTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSV
Sbjct: 121 FTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSV 180

Query: 181 VDMLEKWSAMSAASGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLAS 240
           VDMLEKWSAMSAASGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLAS
Sbjct: 181 VDMLEKWSAMSAASGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLAS 240

Query: 241 QAFEKVFIPGYRFLPTRTNVNSWRLDREIRKSLLKLIDRRRESSIESSKDLLGLMIRASN 300
           QAFEKVFIPGYRFLPTRTNVNSWRLDREIRKSLLKLIDRRRESSIESSKDLLGLMIRASN
Sbjct: 241 QAFEKVFIPGYRFLPTRTNVNSWRLDREIRKSLLKLIDRRRESSIESSKDLLGLMIRASN 300

Query: 301 TSPSSTITVNDIVEECKGFFF 322
           TSPSSTITVNDIVEECKGFFF
Sbjct: 301 TSPSSTITVNDIVEECKGFFF 321

[Close]

BLAST of Carg17521-RA vs. NCBI nr
Match: XP_022965676.1 (cytochrome P450 734A1-like [Cucurbita maxima])

HSP 1 Score: 644.0 bits (1660), Expect = 6.7e-181
Identity = 319/321 (99.38%), Postives = 321/321 (100.00%), Query Frame = 0


Query: 1   MVGGVLEWIKVSILGCFLVVVALRAAVVLWWRPRRIEDHFFRQGIRGPPYRFFIGNVKEL 60
           MVGGVLEWIKVSILGCFLVVVALRAAVVLWWRPR+IEDHFFRQGIRGPPYRFFIGNVKEL
Sbjct: 1   MVGGVLEWIKVSILGCFLVVVALRAAVVLWWRPRKIEDHFFRQGIRGPPYRFFIGNVKEL 60

Query: 61  VGMMIKASSDHSFPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREI 120
           VGMMIKASSDHSFPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREI
Sbjct: 61  VGMMIKASSDHSFPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREI 120

Query: 121 FTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSV 180
           FTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSV
Sbjct: 121 FTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSV 180

Query: 181 VDMLEKWSAMSAASGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLAS 240
           V+MLEKWSAMSAASGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLAS
Sbjct: 181 VEMLEKWSAMSAASGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLAS 240

Query: 241 QAFEKVFIPGYRFLPTRTNVNSWRLDREIRKSLLKLIDRRRESSIESSKDLLGLMIRASN 300
           QAFEKVFIPGYRFLPTRTNVNSWRLDREIRKSLLKLIDRRRESSIESSKDLLGLMIRASN
Sbjct: 241 QAFEKVFIPGYRFLPTRTNVNSWRLDREIRKSLLKLIDRRRESSIESSKDLLGLMIRASN 300

Query: 301 TSPSSTITVNDIVEECKGFFF 322
           TSPSSTITVNDIVEECKGFFF
Sbjct: 301 TSPSSTITVNDIVEECKGFFF 321

[Close]

BLAST of Carg17521-RA vs. ExPASy Swiss-Prot
Match: O48786 (Cytochrome P450 734A1 OS=Arabidopsis thaliana OX=3702 GN=CYP734A1 PE=2 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 2.6e-127
Identity = 226/321 (70.40%), Postives = 268/321 (83.49%), Query Frame = 0


Query: 10  KVSILGCFLVVVALRAAVVLWWRPRRIEDHFFRQGIRGPPYRFFIGNVKELVGMMIKASS 69
           KV +L   L +V ++   +LWWRPR+IE+HF +QGIRGPPY FFIGNVKELVGMM+KASS
Sbjct: 12  KVLVLSVILSLVIVKGMSLLWWRPRKIEEHFSKQGIRGPPYHFFIGNVKELVGMMLKASS 71

Query: 70  DHSFPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCE 129
            H  P FSHNILPRVL FYHHW+KIYG+ FLVWFGPT RL V+DPDLIREIF SKSEF E
Sbjct: 72  -HPMP-FSHNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDLIREIF-SKSEFYE 131

Query: 130 KNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMLEKWSA 189
           KNE HPLVKQLEGDGLLSLKG+KWA HRKIISP+FHM+NLKLL+PV+ KSV DM++KWS 
Sbjct: 132 KNEAHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVVLKSVTDMVDKWSD 191

Query: 190 MSAASGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLASQAFEKVFIP 249
             + +GEVE++V EWFQ LTEDVI+RTAFGSSYEDG+A+FRLQAQQM+L ++AF+KVFIP
Sbjct: 192 KLSENGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFIP 251

Query: 250 GYRFLPTRTNVNSWRLDREIRKSLLKLIDRRRESSIE---------SSKDLLGLMIRASN 309
           GYRF PTR N+ SW+LD+EIRKSLLKLI+RRR+++I+         ++KDLLGLMI+A N
Sbjct: 252 GYRFFPTRGNLKSWKLDKEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMIQAKN 311

Query: 310 TSPSSTITVNDIVEECKGFFF 322
                 +TV DIVEECK FFF
Sbjct: 312 ------VTVQDIVEECKSFFF 323

[Close]

BLAST of Carg17521-RA vs. ExPASy Swiss-Prot
Match: B9X287 (Cytochrome P450 734A6 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A6 PE=2 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 3.7e-110
Identity = 191/317 (60.25%), Postives = 249/317 (78.55%), Query Frame = 0


Query: 21  VALRAAVVLWWRPRRIEDHFFRQGIRGPPYRFFIGNVKELVGMMIKASSDHSFPNF-SHN 80
           VA++   VLWWRPRR+E+HF RQGI GP YRF +G V+E+V +M+ AS+    P + SHN
Sbjct: 17  VAVKVLEVLWWRPRRVEEHFARQGITGPRYRFLVGCVREMVALMVAASAKPMPPPYRSHN 76

Query: 81  ILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCEKNEPHPLVKQ 140
           +LPRVL FYHHWKKIYGS FL+WFGPT RLA++DP+LIRE+  ++++  ++ E HP+V+Q
Sbjct: 77  VLPRVLAFYHHWKKIYGSTFLIWFGPTPRLAIADPELIREVLLARADRFDRYESHPMVRQ 136

Query: 141 LEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMLEKWSAMSAA--SGEV 200
           LEG+GL+SL+G KWA HR++++P+FHMDNL+LL+P +  +V+DM +KW AM+ A  SGEV
Sbjct: 137 LEGEGLVSLRGDKWAHHRRVLTPAFHMDNLRLLLPCVGMTVLDMADKWRAMAEADKSGEV 196

Query: 201 EIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLASQAFEKVFIPGYRFLPTR 260
           EI+VS+WFQ +TED ITRTAFG SYEDGK +F+LQAQ M  AS+AF KVFIPGYRFLPT+
Sbjct: 197 EIDVSDWFQVVTEDAITRTAFGRSYEDGKVVFKLQAQLMAFASEAFRKVFIPGYRFLPTK 256

Query: 261 TNVNSWRLDREIRKSLLKLIDRRRESSIES-----SKDLLGLMIRASNTS--------PS 320
            N +SW+LD+EIRK+L+ LI RR+E+  +      +KDLLGLMI A+ +S        P 
Sbjct: 257 KNTSSWKLDKEIRKNLVTLIGRRQEAGDDEKLDGCAKDLLGLMINAAASSNGGKRSALPV 316

Query: 321 STITVNDIVEECKGFFF 322
           S ITVNDIVEECK FFF
Sbjct: 317 SPITVNDIVEECKTFFF 333

[Close]

BLAST of Carg17521-RA vs. ExPASy Swiss-Prot
Match: Q6Z6D6 (Cytochrome P450 734A2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A2 PE=2 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 4.6e-100
Identity = 176/322 (54.66%), Postives = 231/322 (71.74%), Query Frame = 0


Query: 21  VALRAAVVLWWRPRRIEDHFFRQGIRGPPYRFFIGNVKELVGMMIKASSDHSFPNFSHNI 80
           VA R A  LWWRPRR+E HF  QG+RGPPYRF +G+V+E+V +M +ASS    P  SHN 
Sbjct: 33  VAARMADALWWRPRRLEAHFAAQGVRGPPYRFLLGSVREMVALMAEASSKPMSPPTSHNA 92

Query: 81  LPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCEKNEPHPLVKQL 140
           LPRVL FYH+W+KIYG +FL+WFGPT RL V++P+LIREIF ++++  ++ E HP+V+QL
Sbjct: 93  LPRVLAFYHYWRKIYGHRFLIWFGPTPRLTVAEPELIREIFLTRADAFDRYEAHPVVRQL 152

Query: 141 EGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMLEKWSAMSAA--SGEVE 200
           EGDGL+SL G KWALHR++++ +F+ DNL  LIP + KSV  +  KW AM+ A  SGEVE
Sbjct: 153 EGDGLVSLHGDKWALHRRVLTDAFYPDNLNRLIPHVGKSVAALAAKWGAMAEAGGSGEVE 212

Query: 201 IEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLASQAFEKVFIPGYRFLPTRT 260
           ++V+EWFQ +TE+ ITR  FG SY+DG+ +F +Q Q M  AS+AF KV +PGYRFLPT+ 
Sbjct: 213 VDVAEWFQAVTEEAITRATFGRSYDDGRVVFAMQGQLMAFASEAFRKVLVPGYRFLPTKK 272

Query: 261 NVNSWRLDREIRKSLLKLIDRRRESSIESSK-------DLLGLMIRA------------S 320
           N  SWRLDREIR+SL++LI RR + + +  K       DLLGLMI A             
Sbjct: 273 NRLSWRLDREIRRSLMRLIGRRSDEAEQGEKADDGSFRDLLGLMINAGAAAATRGNAGGE 332

Query: 321 NTSPSSTITVNDIVEECKGFFF 322
             SP++ I V D++EECK FFF
Sbjct: 333 KNSPAAAIPVEDMLEECKTFFF 354

[Close]

BLAST of Carg17521-RA vs. ExPASy Swiss-Prot
Match: Q69XM6 (Cytochrome P450 734A4 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A4 PE=2 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 3.8e-94
Identity = 167/333 (50.15%), Postives = 228/333 (68.47%), Query Frame = 0


Query: 5   VLEWIKVSILGCFLVVVALRAAVVLWWRPRRIEDHFFRQGIRGPPYRFFIGNVKELVGMM 64
           ++E + V+     L+ VA R A  +WWRPRR+E HF  QG+RGPPYRF +G V+E+V +M
Sbjct: 1   MMEAVAVAAAVLLLLHVAARVADAVWWRPRRLEAHFAGQGVRGPPYRFLVGCVREMVALM 60

Query: 65  IKASSDHSFPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSK 124
            +A++    P   HN LPRVL FYH+W+KIYG  FL+WFGPT RL V++P+++REIF ++
Sbjct: 61  AEATAKPMPPAAPHNALPRVLAFYHYWRKIYGPTFLIWFGPTPRLTVAEPEMVREIFLTR 120

Query: 125 SEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDML 184
           +E  ++ E HP+V+QLEGDGL+SL G KWA HR++++P F+ DNL  L+P + +SV  + 
Sbjct: 121 AEAFDRYEAHPVVRQLEGDGLVSLHGDKWAHHRRVLTPGFYPDNLNRLVPHVGRSVAALA 180

Query: 185 EKWSAMS-AASGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLASQAF 244
           E+W AM+ A  GEVE++V+EWFQ + E+ ITR  FG SY+ G+ +FRLQA+ M  AS+AF
Sbjct: 181 ERWRAMACAGGGEVEVDVAEWFQAVAEEAITRATFGRSYDSGRVVFRLQARLMAFASEAF 240

Query: 245 EKVFIPGYRFLPTRTNVNSWRLDREIRKSLLKLIDRR---------------RESSIESS 304
            KV +PGYRFLPT+ N  SW LDREIR+ L++LI RR               ++      
Sbjct: 241 RKVLVPGYRFLPTKKNRMSWGLDREIRRGLVRLIGRRSGGDGGEEDETTTELKDKQDSGF 300

Query: 305 KDLLGLMIRASNTSPSSTITVNDIVEECKGFFF 322
            DLLGLMI   N     T+ V D+VEECK FFF
Sbjct: 301 NDLLGLMI---NAGVDRTMPVEDMVEECKTFFF 330

[Close]

BLAST of Carg17521-RA vs. ExPASy Swiss-Prot
Match: Q8LIF2 (Cytochrome P450 734A5 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A5 PE=2 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 3.8e-78
Identity = 143/308 (46.43%), Postives = 206/308 (66.88%), Query Frame = 0


Query: 22  ALRAAVVLWWRPRRIEDHFFRQGIRGPPYRFFIGNVKELVGMMIKASSDHSFPNFSHNIL 81
           A RAA  LWWRPRR+E HF  QG+RGP YRFF+G+  ELV +M+ A+S    P  SH+IL
Sbjct: 32  AARAAEALWWRPRRVERHFAAQGVRGPGYRFFVGSSIELVRLMVDAASRPMEPPTSHDIL 91

Query: 82  PRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCEKNEPHPLVKQLE 141
           PRVLPFYHHW+K+YG   L+WFG T RL VS+P+LIRE+  ++++  ++ E HP++ Q E
Sbjct: 92  PRVLPFYHHWRKLYGPMHLIWFGRTPRLVVSEPELIREVLLTRADHFDRYEAHPMICQFE 151

Query: 142 GDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMLEKWS--AMSAASGEVEI 201
           G GL +L G++WA  R++++P+FH +NL+++ P +A +V  ML++ +  A +  +GE E+
Sbjct: 152 GYGLSNLHGERWARRRRVLTPAFHTENLRMIAPFVAGTVTRMLDELAERARAGGAGEAEV 211

Query: 202 EVSEWFQTLTEDVITRTAFG-SSYEDGKAIFRLQAQQMVLASQAFEKVFIPGYRFLPTRT 261
           +V+EWFQ + ++ IT  AFG  +Y+DG A+FRLQ +    A++A  KV+IPGYRFLPTR 
Sbjct: 212 DVAEWFQRVPQEAITFAAFGRRNYDDGAAVFRLQDELAGYATEAHSKVYIPGYRFLPTRK 271

Query: 262 NVNSWRLDREIRKSLLKLIDRRRESSIESSKDL-----LGLMIRASNTSPSSTITVNDIV 321
           N   W+LDREIR  L K +   +  S     D       G  +R   +  +  +T  +I+
Sbjct: 272 NRRVWQLDREIRSHLAKFVTGLQSCSSSHGDDADDGGDGGGGMREFMSFMAPAMTAGEII 331

[Close]

BLAST of Carg17521-RA vs. ExPASy TrEMBL
Match: A0A6J1FI14 (cytochrome P450 734A1-like OS=Cucurbita moschata OX=3662 GN=LOC111444133 PE=3 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 5.0e-182
Identity = 321/321 (100.00%), Postives = 321/321 (100.00%), Query Frame = 0


Query: 1   MVGGVLEWIKVSILGCFLVVVALRAAVVLWWRPRRIEDHFFRQGIRGPPYRFFIGNVKEL 60
           MVGGVLEWIKVSILGCFLVVVALRAAVVLWWRPRRIEDHFFRQGIRGPPYRFFIGNVKEL
Sbjct: 1   MVGGVLEWIKVSILGCFLVVVALRAAVVLWWRPRRIEDHFFRQGIRGPPYRFFIGNVKEL 60

Query: 61  VGMMIKASSDHSFPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREI 120
           VGMMIKASSDHSFPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREI
Sbjct: 61  VGMMIKASSDHSFPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREI 120

Query: 121 FTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSV 180
           FTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSV
Sbjct: 121 FTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSV 180

Query: 181 VDMLEKWSAMSAASGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLAS 240
           VDMLEKWSAMSAASGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLAS
Sbjct: 181 VDMLEKWSAMSAASGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLAS 240

Query: 241 QAFEKVFIPGYRFLPTRTNVNSWRLDREIRKSLLKLIDRRRESSIESSKDLLGLMIRASN 300
           QAFEKVFIPGYRFLPTRTNVNSWRLDREIRKSLLKLIDRRRESSIESSKDLLGLMIRASN
Sbjct: 241 QAFEKVFIPGYRFLPTRTNVNSWRLDREIRKSLLKLIDRRRESSIESSKDLLGLMIRASN 300

Query: 301 TSPSSTITVNDIVEECKGFFF 322
           TSPSSTITVNDIVEECKGFFF
Sbjct: 301 TSPSSTITVNDIVEECKGFFF 321

[Close]

BLAST of Carg17521-RA vs. ExPASy TrEMBL
Match: A0A6J1HMB5 (cytochrome P450 734A1-like OS=Cucurbita maxima OX=3661 GN=LOC111465504 PE=3 SV=1)

HSP 1 Score: 644.0 bits (1660), Expect = 3.2e-181
Identity = 319/321 (99.38%), Postives = 321/321 (100.00%), Query Frame = 0


Query: 1   MVGGVLEWIKVSILGCFLVVVALRAAVVLWWRPRRIEDHFFRQGIRGPPYRFFIGNVKEL 60
           MVGGVLEWIKVSILGCFLVVVALRAAVVLWWRPR+IEDHFFRQGIRGPPYRFFIGNVKEL
Sbjct: 1   MVGGVLEWIKVSILGCFLVVVALRAAVVLWWRPRKIEDHFFRQGIRGPPYRFFIGNVKEL 60

Query: 61  VGMMIKASSDHSFPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREI 120
           VGMMIKASSDHSFPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREI
Sbjct: 61  VGMMIKASSDHSFPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREI 120

Query: 121 FTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSV 180
           FTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSV
Sbjct: 121 FTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSV 180

Query: 181 VDMLEKWSAMSAASGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLAS 240
           V+MLEKWSAMSAASGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLAS
Sbjct: 181 VEMLEKWSAMSAASGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLAS 240

Query: 241 QAFEKVFIPGYRFLPTRTNVNSWRLDREIRKSLLKLIDRRRESSIESSKDLLGLMIRASN 300
           QAFEKVFIPGYRFLPTRTNVNSWRLDREIRKSLLKLIDRRRESSIESSKDLLGLMIRASN
Sbjct: 241 QAFEKVFIPGYRFLPTRTNVNSWRLDREIRKSLLKLIDRRRESSIESSKDLLGLMIRASN 300

Query: 301 TSPSSTITVNDIVEECKGFFF 322
           TSPSSTITVNDIVEECKGFFF
Sbjct: 301 TSPSSTITVNDIVEECKGFFF 321

[Close]

BLAST of Carg17521-RA vs. ExPASy TrEMBL
Match: A0A0A0LIW8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G006030 PE=3 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 3.3e-162
Identity = 291/308 (94.48%), Postives = 297/308 (96.43%), Query Frame = 0


Query: 17  FLVVVALRAAVVLWWRPRRIEDHFFRQGIRGPPYRFFIGNVKELVGMMIKASSDHSFPNF 76
           FL V+ LRA VVLWWRPRRIE HFFRQGIRGPPYRFFIGNVKELVGMMIKASSDHSFPN 
Sbjct: 10  FLFVLVLRAVVVLWWRPRRIEQHFFRQGIRGPPYRFFIGNVKELVGMMIKASSDHSFPNS 69

Query: 77  SHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCEKNEPHPL 136
           SHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCEKNEPHPL
Sbjct: 70  SHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCEKNEPHPL 129

Query: 137 VKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMLEKWSAM--SAAS 196
           VKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMLEKWSA+  SA S
Sbjct: 130 VKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMLEKWSALMTSADS 189

Query: 197 GEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLASQAFEKVFIPGYRFL 256
            EVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLASQAFEKVFIPGYRFL
Sbjct: 190 DEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLASQAFEKVFIPGYRFL 249

Query: 257 PTRTNVNSWRLDREIRKSLLKLIDRRRESSIE-SSKDLLGLMIRASNTSPSSTITVNDIV 316
           PTRTNVNSWRLD+EIRKSL+KLIDRRRE+SIE SSKDLLGLMIRAS +SPSSTITVNDIV
Sbjct: 250 PTRTNVNSWRLDKEIRKSLMKLIDRRRENSIETSSKDLLGLMIRASKSSPSSTITVNDIV 309

Query: 317 EECKGFFF 322
           EECKGFFF
Sbjct: 310 EECKGFFF 317

[Close]

BLAST of Carg17521-RA vs. ExPASy TrEMBL
Match: A0A6J1G3R6 (cytochrome P450 734A1-like OS=Cucurbita moschata OX=3662 GN=LOC111450496 PE=3 SV=1)

HSP 1 Score: 578.9 bits (1491), Expect = 1.3e-161
Identity = 288/310 (92.90%), Postives = 298/310 (96.13%), Query Frame = 0


Query: 13  ILGCFLVVVALRAAVVLWWRPRRIEDHFFRQGIRGPPYRFFIGNVKELVGMMIKASSDHS 72
           IL  FL ++ LRAAVVLWWRPR+IE HF RQGIRGPPYRFFIGNVKE+VGMMIKASSDHS
Sbjct: 5   ILCSFLFLLGLRAAVVLWWRPRKIEHHFCRQGIRGPPYRFFIGNVKEIVGMMIKASSDHS 64

Query: 73  FPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCEKNE 132
           FPN SHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCEKNE
Sbjct: 65  FPNSSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCEKNE 124

Query: 133 PHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMLEKWSAMSA 192
           PHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVV+MLEKWSAM A
Sbjct: 125 PHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVEMLEKWSAMLA 184

Query: 193 ASGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLASQAFEKVFIPGYR 252
            SGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLASQAF+KV+IPGYR
Sbjct: 185 DSGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLASQAFDKVYIPGYR 244

Query: 253 FLPTRTNVNSWRLDREIRKSLLKLIDRRRESSIE-SSKDLLGLMIRASNTSPSSTITVND 312
           FLPTRTNVNSWRLDREIRKSL+KLIDRR E+SIE SSKDLLGLMIRAS +SPSSTITVND
Sbjct: 245 FLPTRTNVNSWRLDREIRKSLMKLIDRRHENSIESSSKDLLGLMIRASKSSPSSTITVND 304

Query: 313 IVEECKGFFF 322
           IVEECKGFFF
Sbjct: 305 IVEECKGFFF 314

[Close]

BLAST of Carg17521-RA vs. ExPASy TrEMBL
Match: A0A1S3C777 (cytochrome P450 734A1 OS=Cucumis melo OX=3656 GN=LOC103497465 PE=3 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 8.2e-161
Identity = 289/308 (93.83%), Postives = 296/308 (96.10%), Query Frame = 0


Query: 17  FLVVVALRAAVVLWWRPRRIEDHFFRQGIRGPPYRFFIGNVKELVGMMIKASSDHSFPNF 76
           FL V+ LRA VVLWWRPRRIE HFFRQGIRGPPYRFFIGNVKELVGMMIKASSDHSFPN 
Sbjct: 10  FLFVLLLRAVVVLWWRPRRIEQHFFRQGIRGPPYRFFIGNVKELVGMMIKASSDHSFPNS 69

Query: 77  SHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCEKNEPHPL 136
           SHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCEKNEPHPL
Sbjct: 70  SHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCEKNEPHPL 129

Query: 137 VKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMLEKWSAM--SAAS 196
           VKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMLEKWSA+  SA S
Sbjct: 130 VKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMLEKWSALMTSADS 189

Query: 197 GEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLASQAFEKVFIPGYRFL 256
            EVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLASQAFEKVFIPGYRFL
Sbjct: 190 DEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLASQAFEKVFIPGYRFL 249

Query: 257 PTRTNVNSWRLDREIRKSLLKLIDRRRESSIE-SSKDLLGLMIRASNTSPSSTITVNDIV 316
           PTRTNVNSW+L R+IRKSL+KLIDRRRE+SIE SSKDLLGLMIRAS +SPSSTITVNDIV
Sbjct: 250 PTRTNVNSWKLGRKIRKSLMKLIDRRRENSIETSSKDLLGLMIRASKSSPSSTITVNDIV 309

Query: 317 EECKGFFF 322
           EECKGFFF
Sbjct: 310 EECKGFFF 317

[Close]

BLAST of Carg17521-RA vs. TAIR 10
Match: AT2G26710.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 456.4 bits (1173), Expect = 1.8e-128
Identity = 226/321 (70.40%), Postives = 268/321 (83.49%), Query Frame = 0


Query: 10  KVSILGCFLVVVALRAAVVLWWRPRRIEDHFFRQGIRGPPYRFFIGNVKELVGMMIKASS 69
           KV +L   L +V ++   +LWWRPR+IE+HF +QGIRGPPY FFIGNVKELVGMM+KASS
Sbjct: 12  KVLVLSVILSLVIVKGMSLLWWRPRKIEEHFSKQGIRGPPYHFFIGNVKELVGMMLKASS 71

Query: 70  DHSFPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCE 129
            H  P FSHNILPRVL FYHHW+KIYG+ FLVWFGPT RL V+DPDLIREIF SKSEF E
Sbjct: 72  -HPMP-FSHNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDLIREIF-SKSEFYE 131

Query: 130 KNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMLEKWSA 189
           KNE HPLVKQLEGDGLLSLKG+KWA HRKIISP+FHM+NLKLL+PV+ KSV DM++KWS 
Sbjct: 132 KNEAHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVVLKSVTDMVDKWSD 191

Query: 190 MSAASGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLASQAFEKVFIP 249
             + +GEVE++V EWFQ LTEDVI+RTAFGSSYEDG+A+FRLQAQQM+L ++AF+KVFIP
Sbjct: 192 KLSENGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFIP 251

Query: 250 GYRFLPTRTNVNSWRLDREIRKSLLKLIDRRRESSIE---------SSKDLLGLMIRASN 309
           GYRF PTR N+ SW+LD+EIRKSLLKLI+RRR+++I+         ++KDLLGLMI+A N
Sbjct: 252 GYRFFPTRGNLKSWKLDKEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMIQAKN 311

Query: 310 TSPSSTITVNDIVEECKGFFF 322
                 +TV DIVEECK FFF
Sbjct: 312 ------VTVQDIVEECKSFFF 323

[Close]

BLAST of Carg17521-RA vs. TAIR 10
Match: AT1G75130.1 (cytochrome P450, family 721, subfamily A, polypeptide 1 )

HSP 1 Score: 215.3 bits (547), Expect = 7.1e-56
Identity = 113/295 (38.31%), Postives = 170/295 (57.63%), Query Frame = 0


Query: 31  WRPRRIEDHFFRQGIRGPPYRFFIGNVKELVGMMIKASSDHSFPN--FSHNILPRVLPFY 90
           W P RI+ HF +Q + GP YR F GN  E+  +  +A S    P+    H  + RV P Y
Sbjct: 24  WVPWRIQSHFKKQSVTGPSYRIFSGNSGEVSRLTAEAKS-KPIPSGRNPHEFVHRVAPHY 83

Query: 91  HHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCEKNEPHPLVKQLEGDGLLSL 150
           H W ++YG  FL WFG    +A SDP LIRE  T+   F ++   +PL K L   GL  L
Sbjct: 84  HEWSRVYGKTFLYWFGSKPVVATSDPRLIREALTTGGSF-DRIGHNPLSKLLYAQGLPGL 143

Query: 151 KGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMLEKWSAMSAASGEVEIEVSEWFQTL 210
           +G +WA HR+I   +F M+ LK  +P M  S + ++EKW  M     E+E+EV +    L
Sbjct: 144 RGDQWAFHRRIAKQAFTMEKLKRWVPQMVTSTMMLMEKWEDMRNGGEEIELEVHKEMHNL 203

Query: 211 TEDVITRTAFGSSYEDGKAIFRLQAQQMVLASQAFEKVFIPGYRFLPTRTNVNSWRLDRE 270
           + ++++RTAFG+S E+GK IF LQ + M L       V+IPG+RF P++TN   WR++++
Sbjct: 204 SAEMLSRTAFGNSVEEGKGIFELQERMMRLFYLVRWSVYIPGFRFFPSKTNREIWRIEKQ 263

Query: 271 IRKSLLKLIDRRRESSIESSKDLLGLMIR--ASNTSPSSTITVNDIVEECKGFFF 322
           IR S+LKLI+  + +++E S  LL   +    +       + + ++ +ECK F+F
Sbjct: 264 IRVSILKLIENNK-TAVEKSGTLLQAFMSPYTNQNGQEEKLGIEEVTDECKTFYF 315

[Close]

BLAST of Carg17521-RA vs. TAIR 10
Match: AT2G46950.1 (cytochrome P450, family 709, subfamily B, polypeptide 2 )

HSP 1 Score: 211.1 bits (536), Expect = 1.3e-54
Identity = 116/322 (36.02%), Postives = 185/322 (57.45%), Query Frame = 0


Query: 5   VLEWIKVSILGCFLVVV--ALRAAVVLWWRPRRIEDHFFRQGIRGPPYRFFIGNVKELVG 64
           +L  I +  +   L+VV     A  +L WRP  +   F +QGI GP YR   GN++E+  
Sbjct: 58  LLSTINLLAIALVLLVVPKIYGACRILVWRPWMLSRRFKKQGISGPKYRILYGNLREIRK 117

Query: 65  MMIKASSDHSFPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFT 124
           M  +A      PN S++I+PRVLP    WK  YG  FL W G   RL +SD +L ++I +
Sbjct: 118 MKNEAKLMVLDPN-SNDIVPRVLPHLQQWKSQYGETFLYWQGTDPRLCISDHELAKQILS 177

Query: 125 SKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVD 184
           +K  F  K++  P + +L G+GL+ + G  W  HR+I++P+F MD LKL+  +M      
Sbjct: 178 NKFVFFSKSKTKPEILKLSGNGLIFVNGLDWVRHRRILNPAFSMDKLKLMTQLMVDCTFR 237

Query: 185 MLEKWSAM-SAASGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLASQ 244
           M  +W    +    E  + +S  F+ LT D+I   AFGSSY +G  +F+ Q +     + 
Sbjct: 238 MFLEWKKQRNGVETEQFVLISREFKRLTADIIATAAFGSSYAEGIEVFKSQLELQKCCAA 297

Query: 245 AFEKVFIPGYRFLPTRTNVNSWRLDREIRKSLLKLIDRRRES-SIESSKDLLGLMIRASN 304
           A   ++ PG ++LPT +N+  W+LD ++  S+ ++ID R  S S +   DLLG+M+ A++
Sbjct: 298 ALTDLYFPGIQYLPTPSNLQIWKLDMKVNSSIKRIIDARLTSESKDYGNDLLGIMLTAAS 357

Query: 305 TSPS-STITVNDIVEECKGFFF 322
           ++ S   +++++I+EECK FFF
Sbjct: 358 SNESEKKMSIDEIIEECKTFFF 378

[Close]

BLAST of Carg17521-RA vs. TAIR 10
Match: AT3G14610.1 (cytochrome P450, family 72, subfamily A, polypeptide 7 )

HSP 1 Score: 210.7 bits (535), Expect = 1.8e-54
Identity = 120/322 (37.27%), Postives = 191/322 (59.32%), Query Frame = 0


Query: 17  FLVVVALR---AAVVLW-WR--------PRRIEDHFFRQGIRGPPYRFFIGNVKELVGMM 76
           F VV AL    A VVLW WR        P+ +E    RQG+ G PY   +G++K  V MM
Sbjct: 3   FSVVAALPVLVAVVVLWTWRIVKWVWIKPKMLESSLKRQGLTGTPYTPLVGDIKRNVDMM 62

Query: 77  IKASSDHSFPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSK 136
           ++A S     N + +I PR+LP        +G  F +W GP   + +++P+ I+E+F   
Sbjct: 63  MEARSKPI--NVTDDITPRLLPLALKMLNSHGKTFFIWIGPLPTIVITNPEQIKEVFNKV 122

Query: 137 SEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDML 196
           ++F EK    PL++ L G GL S KG KWA HR+II+P+FH++ +K +IP       +++
Sbjct: 123 NDF-EKASTFPLIRLLAG-GLASYKGDKWASHRRIINPAFHLEKIKNMIPAFYHCCSEVV 182

Query: 197 EKWSAM-SAASGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLASQAF 256
            +W  + +     +E++V  W   +T DVI+ TAFGSSY++G+ IF+LQ +   L +QAF
Sbjct: 183 CQWEKLFTDKESPLEVDVWPWLVNMTADVISHTAFGSSYKEGQRIFQLQGELAELIAQAF 242

Query: 257 EKVFIPGYRFLPTRTNVNSWRLDREIRKSLLKLIDRR---RESSIESSKDLLGLMIRA-S 316
           +K +IPG RF PT++N     +DRE+   L  ++ +R   RE+   ++ DLLG+++ + S
Sbjct: 243 KKSYIPGSRFYPTKSNRRMKAIDREVDVILRGIVSKREKAREAGEPANDDLLGILLESNS 302

Query: 317 NTSPSSTITVNDIVEECKGFFF 322
             S  + ++V D+++ECK F+F
Sbjct: 303 EESQGNGMSVEDVMKECKLFYF 320

[Close]

BLAST of Carg17521-RA vs. TAIR 10
Match: AT2G46960.2 (cytochrome P450, family 709, subfamily B, polypeptide 1 )

HSP 1 Score: 206.1 bits (523), Expect = 4.3e-53
Identity = 109/324 (33.64%), Postives = 187/324 (57.72%), Query Frame = 0


Query: 4   GVLEWIKVSILGCFLVVVALRAAVVLWWRPRRIEDHFFRQGIRGPPYRFFIGNVKELVGM 63
           G++ ++ + +L   + +   +A ++L W P  +      QGI GP YR F GN+ E+  M
Sbjct: 2   GLVIFLALIVLILIIGLRIFKAFMILVWHPFVLTRRLKNQGISGPNYRIFYGNLSEIKKM 61

Query: 64  MIKASSDHSFPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTS 123
             ++      P+ S++I PR+LP Y  W   YG  FL W G   R+ +SDP+L + + ++
Sbjct: 62  KRESHLSILDPS-SNDIFPRILPHYQKWMSQYGETFLYWNGTEPRICISDPELAKTMLSN 121

Query: 124 KSEFCEKNEPHP-LVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVD 183
           K  F  K++  P  VK +   GL+ ++G  W  HR+I++P+F +D LK++  VM    + 
Sbjct: 122 KLGFFVKSKARPEAVKLVGSKGLVFIEGADWVRHRRILNPAFSIDRLKIMTTVMVDCTLK 181

Query: 184 MLEKWSAMSAAS----GEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVL 243
           MLE+W   S        +++ E++E FQ LT D+I  +AFGSSY +G  +FR Q +    
Sbjct: 182 MLEEWRKESTKEETEHPKIKKEMNEEFQRLTADIIATSAFGSSYVEGIEVFRSQMELKRC 241

Query: 244 ASQAFEKVFIPGYRFLPTRTNVNSWRLDREIRKSLLKLIDRRRESSIESSKDLLGLMIRA 303
            + +  +V IPG ++LPT +N+  W+L+R++  S+ ++I  R +S  +   DLLG++++A
Sbjct: 242 YTTSLNQVSIPGTQYLPTPSNIRVWKLERKMDNSIKRIISSRLQSKSDYGDDLLGILLKA 301

Query: 304 SNT-SPSSTITVNDIVEECKGFFF 322
            NT      +++ +I+ EC+ FFF
Sbjct: 302 YNTEGKERKMSIEEIIHECRTFFF 324

The following BLAST results are available for this feature:

BLAST of Carg17521-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023537030.1	1.0e-181	100.00	cytochrome P450 734A1-like [Cucurbita pepo subsp. pepo]	[more]
KAG7021263.1	1.0e-181	100.00	Cytochrome P450, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022937875.1	1.0e-181	100.00	cytochrome P450 734A1-like [Cucurbita moschata]	[more]
KAG6586408.1	1.0e-181	100.00	Cytochrome P450 734A1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022965676.1	6.7e-181	99.38	cytochrome P450 734A1-like [Cucurbita maxima]	[more]

BLAST of Carg17521-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O48786	2.6e-127	70.40	Cytochrome P450 734A1 OS=Arabidopsis thaliana OX=3702 GN=CYP734A1 PE=2 SV=1	[more]
B9X287	3.7e-110	60.25	Cytochrome P450 734A6 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A6 PE=2 ...	[more]
Q6Z6D6	4.6e-100	54.66	Cytochrome P450 734A2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A2 PE=2 ...	[more]
Q69XM6	3.8e-94	50.15	Cytochrome P450 734A4 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A4 PE=2 ...	[more]
Q8LIF2	3.8e-78	46.43	Cytochrome P450 734A5 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A5 PE=2 ...	[more]

BLAST of Carg17521-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FI14	5.0e-182	100.00	cytochrome P450 734A1-like OS=Cucurbita moschata OX=3662 GN=LOC111444133 PE=3 SV...	[more]
A0A6J1HMB5	3.2e-181	99.38	cytochrome P450 734A1-like OS=Cucurbita maxima OX=3661 GN=LOC111465504 PE=3 SV=1	[more]
A0A0A0LIW8	3.3e-162	94.48	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G006030 PE=3 SV=1	[more]
A0A6J1G3R6	1.3e-161	92.90	cytochrome P450 734A1-like OS=Cucurbita moschata OX=3662 GN=LOC111450496 PE=3 SV...	[more]
A0A1S3C777	8.2e-161	93.83	cytochrome P450 734A1 OS=Cucumis melo OX=3656 GN=LOC103497465 PE=3 SV=1	[more]

BLAST of Carg17521-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G26710.1	1.8e-128	70.40	Cytochrome P450 superfamily protein	[more]
AT1G75130.1	7.1e-56	38.31	cytochrome P450, family 721, subfamily A, polypeptide 1	[more]
AT2G46950.1	1.3e-54	36.02	cytochrome P450, family 709, subfamily B, polypeptide 2	[more]
AT3G14610.1	1.8e-54	37.27	cytochrome P450, family 72, subfamily A, polypeptide 7	[more]
AT2G46960.2	4.3e-53	33.64	cytochrome P450, family 709, subfamily B, polypeptide 1	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002402	Cytochrome P450, E-class, group II	PRINTS	PR00464	EP450II	coord: 150..170 score: 36.21 coord: 207..225 score: 32.64
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 30..321 e-value: 9.8E-46 score: 158.2
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 45..321
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 87..321 e-value: 6.6E-24 score: 84.5
None	No IPR available	PANTHER	PTHR24282:SF63	CYTOCHROMEA2	coord: 7..321
None	No IPR available	PANTHER	PTHR24282	CYTOCHROME P450 FAMILY MEMBER	coord: 7..321

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg17521	Carg17521	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg17521-RA:4	Carg17521-RA:4	exon
Carg17521-RA:3	Carg17521-RA:3	exon
Carg17521-RA:2	Carg17521-RA:2	exon
Carg17521-RA:1	Carg17521-RA:1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg17521-RA:cds	Carg17521-RA:cds_4	CDS
Carg17521-RA:cds	Carg17521-RA:cds_3	CDS
Carg17521-RA:cds	Carg17521-RA:cds_2	CDS
Carg17521-RA:cds	Carg17521-RA:cds	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg17521-RA:five_prime_utr	Carg17521-RA:five_prime_utr	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg17521-RA	Carg17521-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010268	brassinosteroid homeostasis
biological_process	GO:0016131	brassinosteroid metabolic process
biological_process	GO:0009741	response to brassinosteroid
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function	GO:0008395	steroid hydroxylase activity